Rroxscaffold_4G00307360

Phospholipase A(1) DAD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014224
Physical Location & Seq
Forward (+)
28623868 .. 28628590
4723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00307360.1

Sequence Viewer

Length: 1530 bp
ATGAACAAATCAGATTTTAATCTCTTTTTAATGTACTTTATTCCACCTCTTGATTTTTACAAGTTAAATATAGATGGTGCTAAACAGGAGGTTAGCGGTGTGCGCGGTGTTGTAGTAGTTGTGTACGCAGGATCATGCAGGATTGTTATAGGTACTGTAGAAGCAAGGGAGATCTCTGTCCAAGCTACAAAACTCCTAGCTCCTAAAACTGGTTTTGATGAGAGGGTCTCTGTGTCCTTTCCTTCCCTCTCTTTCCCAAAAGTGAGAATGAGGCTCTCCATGATCAAACCCATAAGTGCTACTCCCTGCACACAGCTCTCTACTCCAAAGCTCAAGTCAATGATCGGCTGCTGCACCCTCACTCAATCACCAGCAAAGCCCACCCAGCAAATCAAGTTCACTGATGACTTCCCCAGCTGGGAACAATTGCTCAACCCAGTCCCAGTTGATCTCAACTCCCCAATGAGTGCCTCCACCTCCACATCGACCTCCACCTCCACCTCCACTACTAAAAGACCAATTATTCGGTCGGTCCGAAAGAATTGGATGGAGTACCAAGGAGTCCAGAACTGGGAAGGACTGCTCGACCCGCTGGACGGAAATCTGCGCCGGGAAATTATCCGGTACGGCAATTTCGTTGATGCAGCCTACAAGGCATTTGACTTTGACCCGGATTCACCTACATACGCAACCTGCAAGTATCCCAAAAGTACGGTTTTTGACAACTCCGGACTTCCGGGGACAGGTTACCGGATCACCAAGAATCTACGTGCCACGTCAGGCATTAACCTGCCACGCTGGATCGAAAGGGCACCGAGCTGGGTTGGCACTCAATCCAGCTGGATTGGGTACGTAGCGGTGTGTAAAGACAAAGACGAAATCGCCAGACTTGGACGACGCGATGTCGTCATTGCCTTAAGAGGCACTGCCACATGTTTGGAGTGGCTCGAAAATCTACGAGTCACTCTAACTCATCTCCCGTGTGGGTCTAAAGCTGCTCACACAGGAGGTGCTGAGGGGTCCGAACCAATGGTCGAGAGTGGGTTTTTGAGCCTTTACACTTCGGGAACCGACCTAGTCCCAAGCTTGCAACAAATGATACGTGAAGAAATTGGGAGGTTGCTTCAAACCTACGGCGACGAGCCGCTGAGCCTAACCATCACTGGCCACAGCCTCGGCGCCGCACTCGCAACTCTGGCGGCGTACGACATCAAATCCACATTCAACCGCGCACCACTCGCCACGGTCATATCCTTTGGCGGTCCAAGAGTTGGCAACCGTAGCTTCCGCCACAATCTCGAGAAAAAAGGCGCCAAAGTCTTACGCATTGTGAACGAGGAAGATTTGATTACCAAGGTGCCGGGCTTCGTGGTCGACGACAACCGAGCCCGGCACGTTGCGGGAGTCCCCTCGTCCGTATGGCTGCTGAATTCCAGAATTGGATTCAGAAGCGGGTCGAGGAGACACAGTGGGTTTATTCCGAGGTGGGGAAAGAGCTCAGACTCAGTAGCAAGAACTCGCCGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

509

Amino Acids

56.1

Weight (kDa)

9.62

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 303 - 456 3e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44810 AT2G44810
fragaria_vesca FvH4_7g10210
malus_domestica MD02G1213100.v1.1 MD07G1109300.v1.1
prunus_persica Prupe.2G132000_v2.0.a1 Prupe.2G132100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0347941
rosa_laevigata RLG00000028702
rosa_multiflora Rmu_ssc0000342.1_g000017
rosa_roxburghii Rroxscaffold_4G00307360
rosa_rugosa Rorug01G0190400
rosa_samantha Rh1AG209000 Rh1BG173600 Rh1CG193200 Rh1DG203600
rosa_wichuraiana Rw1G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 701, 798
AccB1I GGYRCC 4 cut(s) 811, 1178, 1310, 1357
AccB7I CCANNNNNTGG 1 cut(s) 1271
AccI GTMKAC 1 cut(s) 1374
AccII CGCG 3 cut(s) 105, 900, 1230
AccIII TCCGGA 1 cut(s) 728
AclWI GGATC 3 cut(s) 139, 761, 809
AcoI YGGCCR 1 cut(s) 1165
AcsI RAATTY 1 cut(s) 1429
AcyI GRCGYC 2 cut(s) 1179, 1311
AfaI GTAC 8 cut(s) 35, 125, 154, 554, 626, 712, 851, 1205
AfiI CCNNNNNNNGG 7 cut(s) 209, 418, 571, 596, 743, 1271, 1487
AflII CTTAAG 1 cut(s) 916
AflIII ACRYGT 1 cut(s) 932
AgsI TTSAA 2 cut(s) 1127, 1225
AhdI GACNNNNNGTC 1 cut(s) 902
AjiI CACGTC 1 cut(s) 777
Alw21I GWGCWC 1 cut(s) 1499
Alw26I GTCTC 2 cut(s) 232, 1456
AlwI GGATC 3 cut(s) 139, 761, 809
Ama87I CYCGRG 1 cut(s) 1298
Aor13HI TCCGGA 1 cut(s) 728
AoxI GGCC 1 cut(s) 1165
ApeKI GCWGC 5 cut(s) 348, 351, 644, 995, 1423
ApoI RAATTY 1 cut(s) 1429
ArsI GACNNNNNNTTYG 6 cut(s) 320, 352, 507, 539, 1017, 1049
AspLEI GCGC 5 cut(s) 105, 609, 1181, 1232, 1313
AspS9I GGNCC 4 cut(s) 532, 1020, 1262, 1524
AsuC2I CCSGG 5 cut(s) 611, 671, 738, 1362, 1390
AsuHPI GGTGA 3 cut(s) 360, 669, 748
AvaI CYCGRG 1 cut(s) 1298
AvaII GGWCC 4 cut(s) 532, 1020, 1262, 1524
BaeGI GKGCMC 1 cut(s) 814
BalI TGGCCA 1 cut(s) 1167
BanI GGYRCC 4 cut(s) 811, 1178, 1310, 1357
BanII GRGCYC 2 cut(s) 1390, 1499
Bbv12I GWGCWC 1 cut(s) 1499
BbvCI CCTCAGC 1 cut(s) 1014
BbvI GCAGC 5 cut(s) 335, 338, 656, 982, 1410
BccI CCATC 3 cut(s) 68, 541, 1166
BceAI ACGGC 2 cut(s) 643, 1150
BciVI GTATCC 1 cut(s) 711
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 5 cut(s) 611, 671, 738, 1362, 1390
BcoDI GTCTC 2 cut(s) 232, 1456
BfaI CTAG 2 cut(s) 197, 1076
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 2 cut(s) 1182, 1314
BfrI CTTAAG 1 cut(s) 916
BfuAI ACCTGC 2 cut(s) 701, 798
BfuI GTATCC 1 cut(s) 711
BglI GCCNNNNNGGC 1 cut(s) 653
BglII AGATCT 1 cut(s) 171
BisI GCNGC 8 cut(s) 349, 352, 645, 996, 1145, 1182, 1200, 1424
BlpI GCTNAGC 1 cut(s) 1148
BlsI GCNGC 8 cut(s) 350, 353, 646, 997, 1146, 1183, 1201, 1425
Bme1390I CCNGG 5 cut(s) 611, 671, 738, 1362, 1390
Bme18I GGWCC 4 cut(s) 532, 1020, 1262, 1524
BmeRI GACNNNNNGTC 1 cut(s) 902
BmeT110I CYCGRG 1 cut(s) 1298
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 4 cut(s) 532, 1020, 1262, 1524
BmiI GGNNCC 6 cut(s) 813, 1021, 1069, 1180, 1312, 1359
BmrFI CCNGG 5 cut(s) 611, 671, 738, 1362, 1390
BmrI ACTGGG 3 cut(s) 431, 437, 580
BmsI GCATC 1 cut(s) 631
BmuI ACTGGG 3 cut(s) 431, 437, 580
BplI GAGNNNNNCTC 2 cut(s) 212, 244
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu1102I GCTNAGC 1 cut(s) 1148
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 5 cut(s) 611, 671, 738, 1362, 1390
BsaAI YACGTR 3 cut(s) 770, 853, 1103
BsaBI GATNNNNATC 1 cut(s) 18
BsaHI GRCGYC 2 cut(s) 1179, 1311
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 6 cut(s) 556, 737, 1174, 1242, 1353, 1481
BsaWI WCCGGW 3 cut(s) 621, 728, 750
BsaXI ACNNNNNCTCC 2 cut(s) 1453, 1483
Bsc4I CCNNNNNNNGG 7 cut(s) 209, 418, 571, 596, 743, 1271, 1487
Bse1I ACTGG 5 cut(s) 214, 437, 443, 575, 1168
Bse3DI GCAATG 1 cut(s) 909
Bse8I GATNNNNATC 1 cut(s) 18
BseAI TCCGGA 1 cut(s) 728
BseDI CCNNGG 6 cut(s) 556, 737, 1174, 1242, 1353, 1481
BseGI GGATG 1 cut(s) 552
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 7 cut(s) 209, 418, 571, 596, 743, 1271, 1487
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 4 cut(s) 1005, 1139, 1512, 1518
BseNI ACTGG 5 cut(s) 214, 437, 443, 575, 1168
BseRI GAGGAG 1 cut(s) 1474
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 5 cut(s) 335, 338, 656, 982, 1410
BseYI CCCAGC 4 cut(s) 384, 413, 417, 819
BsgI GTGCAG 2 cut(s) 292, 337
Bsh1236I CGCG 3 cut(s) 105, 900, 1230
Bsh1285I CGRYCG 1 cut(s) 530
BshFI GGCC 1 cut(s) 1167
BshNI GGYRCC 4 cut(s) 811, 1178, 1310, 1357
BsiEI CGRYCG 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 1499
BsiHKCI CYCGRG 1 cut(s) 1298
BsiSI CCGG 9 cut(s) 610, 622, 671, 729, 737, 751, 1361, 1390, 1522
BsiWI CGTACG 1 cut(s) 1203
BslFI GGGAC 4 cut(s) 425, 754, 1064, 1391
BslI CCNNNNNNNGG 7 cut(s) 209, 418, 571, 596, 743, 1271, 1487
BsmAI GTCTC 2 cut(s) 232, 1456
BsmFI GGGAC 4 cut(s) 425, 754, 1064, 1391
BsnI GGCC 1 cut(s) 1167
Bso31I GGTCTC 1 cut(s) 232
BsoBI CYCGRG 1 cut(s) 1298
Bsp1286I GDGCHC 3 cut(s) 814, 1390, 1499
Bsp13I TCCGGA 1 cut(s) 728
Bsp143I GATC 7 cut(s) 131, 171, 282, 342, 448, 753, 801
Bsp1720I GCTNAGC 1 cut(s) 1148
BspANI GGCC 1 cut(s) 1167
BspCNI CTCAG 4 cut(s) 1006, 1140, 1511, 1517
BspEI TCCGGA 1 cut(s) 728
BspFNI CGCG 3 cut(s) 105, 900, 1230
BspLI GGNNCC 6 cut(s) 813, 1021, 1069, 1180, 1312, 1359
BspMI ACCTGC 2 cut(s) 701, 798
BspPI GGATC 3 cut(s) 139, 761, 809
BspT107I GGYRCC 4 cut(s) 811, 1178, 1310, 1357
BspTI CTTAAG 1 cut(s) 916
BspTNI GGTCTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 909
BsrI ACTGG 5 cut(s) 214, 437, 443, 575, 1168
BssECI CCNNGG 6 cut(s) 556, 737, 1174, 1242, 1353, 1481
BssMI GATC 7 cut(s) 131, 171, 282, 342, 448, 753, 801
BssNI GRCGYC 2 cut(s) 1179, 1311
BssT1I CCWWGG 2 cut(s) 556, 1353
Bst4CI ACNGT 5 cut(s) 157, 715, 1246, 1280, 1469
BstACI GRCGYC 2 cut(s) 1179, 1311
BstAFI CTTAAG 1 cut(s) 916
BstBAI YACGTR 3 cut(s) 770, 853, 1103
BstC8I GCNNGC 1 cut(s) 1088
BstDEI CTNAG 4 cut(s) 1014, 1148, 1498, 1504
BstDSI CCRYGG 1 cut(s) 1242
BstEII GGTNACC 1 cut(s) 746
BstF5I GGATG 1 cut(s) 552
BstFNI CGCG 3 cut(s) 105, 900, 1230
BstH2I RGCGCY 2 cut(s) 1182, 1314
BstHHI GCGC 5 cut(s) 105, 609, 1181, 1232, 1313
BstKTI GATC 7 cut(s) 134, 174, 285, 345, 451, 756, 804
BstMAI GTCTC 2 cut(s) 232, 1456
BstMBI GATC 7 cut(s) 131, 171, 282, 342, 448, 753, 801
BstMCI CGRYCG 1 cut(s) 530
BstMWI GCNNNNNNNGC 9 cut(s) 102, 385, 589, 653, 825, 1187, 1196, 1238, 1281
BstNSI RCATGY 1 cut(s) 936
BstPI GGTNACC 1 cut(s) 746
BstSCI CCNGG 5 cut(s) 609, 669, 736, 1360, 1388
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 814
BstSNI TACGTA 1 cut(s) 853
BstUI CGCG 3 cut(s) 105, 900, 1230
BstV1I GCAGC 5 cut(s) 335, 338, 656, 982, 1410
BstX2I RGATCY 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 937
BstYI RGATCY 1 cut(s) 171
BsuI GTATCC 1 cut(s) 711
BsuRI GGCC 1 cut(s) 1167
BtgI CCRYGG 1 cut(s) 1242
BtgZI GCGATG 1 cut(s) 915
BtrI CACGTC 1 cut(s) 777
BtsCI GGATG 1 cut(s) 552
BtsI GCAGTG 1 cut(s) 924
BtsIMutI CAGTG 4 cut(s) 399, 924, 1161, 1474
BveI ACCTGC 2 cut(s) 701, 798
Cac8I GCNNGC 1 cut(s) 1088
CfoI GCGC 5 cut(s) 105, 609, 1181, 1232, 1313
Cfr13I GGNCC 4 cut(s) 532, 1020, 1262, 1524
CpoI CGGWCCG 1 cut(s) 532
CseI GACGC 1 cut(s) 906
Csp6I GTAC 8 cut(s) 34, 124, 153, 553, 625, 711, 850, 1204
CspI CGGWCCG 1 cut(s) 532
CviAII CATG 3 cut(s) 135, 280, 933
CviQI GTAC 8 cut(s) 34, 124, 153, 553, 625, 711, 850, 1204
DdeI CTNAG 4 cut(s) 1014, 1148, 1498, 1504
DinI GGCGCC 2 cut(s) 1180, 1312
DpnI GATC 7 cut(s) 133, 173, 284, 344, 450, 755, 803
DpnII GATC 7 cut(s) 131, 171, 282, 342, 448, 753, 801
DriI GACNNNNNGTC 1 cut(s) 902
EaeI YGGCCR 1 cut(s) 1165
Eam1105I GACNNNNNGTC 1 cut(s) 902
EciI GGCGGA 1 cut(s) 1277
Ecl136II GAGCTC 1 cut(s) 1497
Eco105I TACGTA 1 cut(s) 853
Eco130I CCWWGG 2 cut(s) 556, 1353
Eco24I GRGCYC 2 cut(s) 1390, 1499
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 4 cut(s) 532, 1020, 1262, 1524
Eco53kI GAGCTC 1 cut(s) 1497
Eco88I CYCGRG 1 cut(s) 1298
Eco91I GGTNACC 1 cut(s) 746
EcoICRI GAGCTC 1 cut(s) 1497
EcoO65I GGTNACC 1 cut(s) 746
EcoRI GAATTC 1 cut(s) 1429
EcoT14I CCWWGG 2 cut(s) 556, 1353
EcoT38I GRGCYC 2 cut(s) 1390, 1499
EgeI GGCGCC 2 cut(s) 1180, 1312
EheI GGCGCC 2 cut(s) 1180, 1312
ErhI CCWWGG 2 cut(s) 556, 1353
FaeI CATG 3 cut(s) 138, 283, 936
FaiI YATR 9 cut(s) 71, 136, 149, 281, 293, 685, 934, 1250, 1420
FaqI GGGAC 4 cut(s) 425, 754, 1064, 1391
FatI CATG 3 cut(s) 134, 279, 932
FauI CCCGC 3 cut(s) 597, 1393, 1445
FbaI TGATCA 1 cut(s) 282
FblI GTMKAC 1 cut(s) 1374
Fnu4HI GCNGC 8 cut(s) 349, 352, 645, 996, 1145, 1182, 1200, 1424
FokI GGATG 1 cut(s) 559
FriOI GRGCYC 2 cut(s) 1390, 1499
Fsp4HI GCNGC 8 cut(s) 349, 352, 645, 996, 1145, 1182, 1200, 1424
FspBI CTAG 2 cut(s) 197, 1076
GlaI GCGC 5 cut(s) 104, 608, 1180, 1231, 1312
GluI GCNGC 8 cut(s) 349, 352, 645, 996, 1145, 1182, 1200, 1424
GsaI CCCAGC 4 cut(s) 388, 417, 421, 823
HaeII RGCGCY 2 cut(s) 1182, 1314
HaeIII GGCC 1 cut(s) 1167
HapII CCGG 9 cut(s) 610, 622, 671, 729, 737, 751, 1361, 1390, 1522
HgaI GACGC 1 cut(s) 906
HhaI GCGC 5 cut(s) 105, 609, 1181, 1232, 1313
Hin1I GRCGYC 2 cut(s) 1179, 1311
Hin1II CATG 3 cut(s) 138, 283, 936
Hin6I GCGC 5 cut(s) 103, 607, 1179, 1230, 1311
HinP1I GCGC 5 cut(s) 103, 607, 1179, 1230, 1311
HincII GTYRAC 1 cut(s) 1375
HindII GTYRAC 1 cut(s) 1375
HindIII AAGCTT 1 cut(s) 1084
HinfI GANTC 7 cut(s) 561, 674, 763, 960, 1404, 1443, 1502
HpaII CCGG 9 cut(s) 610, 622, 671, 729, 737, 751, 1361, 1390, 1522
HphI GGTGA 3 cut(s) 360, 669, 748
Hpy166II GTNNAC 4 cut(s) 124, 399, 1333, 1375
Hpy188I TCNGA 6 cut(s) 13, 536, 1024, 1448, 1482, 1501
Hpy188III TCNNGA 8 cut(s) 50, 565, 729, 1036, 1065, 1298, 1300, 1434
Hpy8I GTNNAC 4 cut(s) 124, 399, 1333, 1375
Hpy99I CGWCG 3 cut(s) 900, 1142, 1379
HpyAV CCTTC 2 cut(s) 252, 569
HpyCH4III ACNGT 5 cut(s) 157, 715, 1246, 1280, 1469
HpyCH4IV ACGT 5 cut(s) 769, 776, 852, 1102, 1395
HpyCH4V TGCA 6 cut(s) 138, 309, 354, 644, 696, 1090
HpyF10VI GCNNNNNNNGC 9 cut(s) 102, 385, 589, 653, 825, 1187, 1196, 1238, 1281
HpyF3I CTNAG 4 cut(s) 1014, 1148, 1498, 1504
HpySE526I ACGT 5 cut(s) 769, 776, 852, 1102, 1395
Hsp92I GRCGYC 2 cut(s) 1179, 1311
Hsp92II CATG 3 cut(s) 138, 283, 936
HspAI GCGC 5 cut(s) 103, 607, 1179, 1230, 1311
KasI GGCGCC 2 cut(s) 1178, 1310
Kpn2I TCCGGA 1 cut(s) 728
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 7 cut(s) 131, 171, 282, 342, 448, 753, 801
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 5 cut(s) 335, 338, 656, 982, 1410
LweI GCATC 1 cut(s) 631
MaeI CTAG 2 cut(s) 197, 1076
MaeII ACGT 5 cut(s) 769, 776, 852, 1102, 1395
MaeIII GTNAC 2 cut(s) 746, 961
MalI GATC 7 cut(s) 133, 173, 284, 344, 450, 755, 803
MboI GATC 7 cut(s) 131, 171, 282, 342, 448, 753, 801
MboII GAAGA 2 cut(s) 1118, 1352
MfeI CAATTG 1 cut(s) 425
MflI RGATCY 1 cut(s) 171
MhlI GDGCHC 3 cut(s) 814, 1390, 1499
MlsI TGGCCA 1 cut(s) 1167
MluCI AATT 8 cut(s) 425, 519, 541, 615, 631, 1110, 1429, 1437
MluNI TGGCCA 1 cut(s) 1167
Mly113I GGCGCC 2 cut(s) 1179, 1311
MlyI GAGTC 4 cut(s) 570, 969, 1413, 1496
Mox20I TGGCCA 1 cut(s) 1167
MroI TCCGGA 1 cut(s) 728
MscI TGGCCA 1 cut(s) 1167
MseI TTAA 5 cut(s) 18, 29, 65, 786, 917
Msp20I TGGCCA 1 cut(s) 1167
MspA1I CMGCKG 4 cut(s) 417, 592, 840, 1147
MspCI CTTAAG 1 cut(s) 916
MspI CCGG 9 cut(s) 610, 622, 671, 729, 737, 751, 1361, 1390, 1522
MspR9I CCNGG 5 cut(s) 611, 671, 738, 1362, 1390
MunI CAATTG 1 cut(s) 425
MvnI CGCG 3 cut(s) 105, 900, 1230
MwoI GCNNNNNNNGC 9 cut(s) 102, 385, 589, 653, 825, 1187, 1196, 1238, 1281
NarI GGCGCC 2 cut(s) 1179, 1311
NciI CCSGG 5 cut(s) 611, 671, 738, 1362, 1390
NdeII GATC 7 cut(s) 131, 171, 282, 342, 448, 753, 801
NlaIII CATG 3 cut(s) 138, 283, 936
NlaIV GGNNCC 6 cut(s) 813, 1021, 1069, 1180, 1312, 1359
NmeAIII GCCGAG 1 cut(s) 1155
NmuCI GTSAC 1 cut(s) 961
NspI RCATGY 1 cut(s) 936
PaeR7I CTCGAG 1 cut(s) 1298
PciI ACATGT 1 cut(s) 932
PcsI WCGNNNNNNNCGW 3 cut(s) 532, 633, 1374
PfeI GAWTC 3 cut(s) 674, 763, 1443
Pfl23II CGTACG 1 cut(s) 1203
PflFI GACNNNGTC 1 cut(s) 1076
PflMI CCANNNNNTGG 1 cut(s) 1271
PkrI GCNGC 8 cut(s) 350, 353, 646, 997, 1146, 1183, 1201, 1425
PleI GAGTC 4 cut(s) 569, 968, 1412, 1496
PluTI GGCGCC 2 cut(s) 1182, 1314
PpsI GAGTC 4 cut(s) 569, 968, 1412, 1496
Ppu21I YACGTR 3 cut(s) 770, 853, 1103
PscI ACATGT 1 cut(s) 932
Psp124BI GAGCTC 1 cut(s) 1499
PspEI GGTNACC 1 cut(s) 746
PspFI CCCAGC 4 cut(s) 384, 413, 417, 819
PspLI CGTACG 1 cut(s) 1203
PspN4I GGNNCC 6 cut(s) 813, 1021, 1069, 1180, 1312, 1359
PspPI GGNCC 4 cut(s) 532, 1020, 1262, 1524
PsuI RGATCY 1 cut(s) 171
PsyI GACNNNGTC 1 cut(s) 1076
PvuII CAGCTG 2 cut(s) 417, 840
RsaI GTAC 8 cut(s) 35, 125, 154, 554, 626, 712, 851, 1205
RsaNI GTAC 8 cut(s) 34, 124, 153, 553, 625, 711, 850, 1204
Rsr2I CGGWCCG 1 cut(s) 532
RsrII CGGWCCG 1 cut(s) 532
SacI GAGCTC 1 cut(s) 1499
SalI GTCGAC 1 cut(s) 1373
SaqAI TTAA 5 cut(s) 18, 29, 65, 786, 917
SatI GCNGC 8 cut(s) 349, 352, 645, 996, 1145, 1182, 1200, 1424
Sau3AI GATC 7 cut(s) 131, 171, 282, 342, 448, 753, 801
Sau96I GGNCC 4 cut(s) 532, 1020, 1262, 1524
SchI GAGTC 4 cut(s) 570, 969, 1413, 1496
ScrFI CCNGG 5 cut(s) 611, 671, 738, 1362, 1390
SduI GDGCHC 3 cut(s) 814, 1390, 1499
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 1 cut(s) 156
SfoI GGCGCC 2 cut(s) 1180, 1312
Sfr274I CTCGAG 1 cut(s) 1298
SinI GGWCC 4 cut(s) 532, 1020, 1262, 1524
SlaI CTCGAG 1 cut(s) 1298
SmlI CTYRAG 3 cut(s) 332, 916, 1298
SmoI CTYRAG 3 cut(s) 332, 916, 1298
SnaBI TACGTA 1 cut(s) 853
Sse9I AATT 8 cut(s) 425, 519, 541, 615, 631, 1110, 1429, 1437
SspDI GGCGCC 2 cut(s) 1178, 1310
SspMI CTAG 2 cut(s) 197, 1076
SstI GAGCTC 1 cut(s) 1499
StyD4I CCNGG 5 cut(s) 609, 669, 736, 1360, 1388
StyI CCWWGG 2 cut(s) 556, 1353
TaaI ACNGT 5 cut(s) 157, 715, 1246, 1280, 1469
TaiI ACGT 5 cut(s) 772, 779, 855, 1105, 1398
TaqI TCGA 8 cut(s) 485, 585, 804, 948, 1035, 1299, 1374, 1457
TaqII GACCGA 2 cut(s) 516, 520
TasI AATT 8 cut(s) 425, 519, 541, 615, 631, 1110, 1429, 1437
TatI WGTACW 1 cut(s) 33
TauI GCSGC 3 cut(s) 1147, 1184, 1202
TfiI GAWTC 3 cut(s) 674, 763, 1443
Tru1I TTAA 5 cut(s) 18, 29, 65, 786, 917
Tru9I TTAA 5 cut(s) 18, 29, 65, 786, 917
TscAI CASTG 4 cut(s) 406, 931, 1168, 1474
TseFI GTSAC 1 cut(s) 961
TseI GCWGC 5 cut(s) 348, 351, 644, 995, 1423
Tsp45I GTSAC 1 cut(s) 961
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 612, 1405
TspRI CASTG 4 cut(s) 406, 931, 1168, 1474
Tth111I GACNNNGTC 1 cut(s) 1076
Van91I CCANNNNNTGG 1 cut(s) 1271
Vha464I CTTAAG 1 cut(s) 916
VpaK11BI GGWCC 4 cut(s) 532, 1020, 1262, 1524
XapI RAATTY 1 cut(s) 1429
XceI RCATGY 1 cut(s) 936
XhoI CTCGAG 1 cut(s) 1298
XmiI GTMKAC 1 cut(s) 1374
XspI CTAG 2 cut(s) 197, 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.