Rh1BG173600

Phospholipase A(1) DAD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0014224
Physical Location & Seq
Reverse (-)
27673728 .. 27676468
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG173600.1

Sequence Viewer

Length: 1374 bp
ATGAAGCTCTCCATGATCAAACCCATAAGTGCTACTCCCTGCACACAGCTCTCTACTCCAAAGCTCAAGTCAATGATCAGCTGCTGCACCCTCACTCAATCACCAGTAAAGCCCACCCAGCAAATCAAGTTCACTGATAACTTCCCCAGGTGGGAACAATTGCTCAACCCAGTCCCAGTTGATCTCAACTCCCCAATGAGTGCCTCCACCTCCACCTCCACCTCCACTACTAAAAGACCAACTATTCGGTCGGTCCGAAAGAATTGGATGGAGTACCAAGGAGTCAAGAACTGGGAAGGACTGCTCGACCCACTGGACGGAAATCTGCGCAGGGAAATTATCCGGTACGGCAATTTCGTCGATGCAGCCTACAAGGCATTTGACTTTGACCCGGATTCACCTACATACGCAACCTGCAAGTATCCCAAAAGTACGGTTTTTGACAACTCCGGACTTCCGGGGACAGGTTACCGGATCACCAAGAATCTACGTGCCACGTCAGGCATTAACCTGCCACGCTGGATCGAAAGGGCACCGAGCTGGGTTGGCACTCAATCCAGCTGGATTGGATACGTGGCGGTGTGTAAAGACAAAGATGAAATCGCCAGACTTGGACGACGCGATGTCGTCATTGCCTTAAGAGGCACCGCCACATGTTTGGAGTGGCTCGAAAATCTCCGAGTCACTCTTACTCATCTCCCGTGTGGGTCTAAAGCTGCTCACACAGGAGGAGCTGAGGGGTCCGAACCAATGGTCGAGAGTGGGTTTTTGAGCCTTTACACTTCGGGAACCGACCTAGTCCCAAGCTTGCAACAAATGATACGTGAAGAAATTGGGAGGTTGCTTCAAACCTACGGCGACGAGCCGCTGAGCCTAACCATCACTGGCCACAGCCTCGGCGCCGCACTCGCAACTCTGGCGGCGTACGACATCAAATCCACATTCAACCGCGCACCACTCGCCACGGTCATATCCTTTGGCGGTCCAAGAGTTGGCAACCGTAGCTTCCGCCACAATCTCGAGAAAAAAGGCGCCAAAGTCTTGCGCATTGTGAACGAGGAAGATTTGATTACCAAAGTGCCGGGCTTCGTGGTCGACGACAACCGAGCCCGGCACGTTGCGGGGAGTCCCCTCGTCCGTATGGCTGCTGAATTCCAGAATTGGATTCAGAAGCGGGTCGAGGAGACACAGTGGGTTTATTCCGAGGTGGGGAAAGAGCTCAGACTCAGTAGCAAGAACTCGCCGGACCTGAGCGCTATGAACATGTCAACATGTCACGATCTCGGAACTTACCTCCACCTCGTGGAAAACTTCGTCAGCGAAAAGTGCCCTTGCAAATCCACCGCAAAGAAGTTGTTGAGTAGAGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

457

Amino Acids

50.72

Weight (kDa)

9.22

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 210 - 363 2.4e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44810 AT2G44810
fragaria_vesca FvH4_7g10210
malus_domestica MD02G1213100.v1.1 MD07G1109300.v1.1
prunus_persica Prupe.2G132000_v2.0.a1 Prupe.2G132100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0347941
rosa_laevigata RLG00000028702
rosa_multiflora Rmu_ssc0000342.1_g000017
rosa_roxburghii Rroxscaffold_4G00307360
rosa_rugosa Rorug01G0190400
rosa_samantha Rh1AG209000 Rh1BG173600 Rh1CG193200 Rh1DG203600
rosa_wichuraiana Rw1G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 329, 1046
Acc36I ACCTGC 2 cut(s) 422, 519
AccB1I GGYRCC 4 cut(s) 532, 644, 899, 1031
AccB7I CCANNNNNTGG 2 cut(s) 992, 1303
AccI GTMKAC 1 cut(s) 1095
AccII CGCG 2 cut(s) 621, 951
AccIII TCCGGA 1 cut(s) 449
AclWI GGATC 2 cut(s) 482, 530
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 1 cut(s) 1151
AcyI GRCGYC 2 cut(s) 900, 1032
AdeI CACNNNGTG 1 cut(s) 1303
AfaI GTAC 5 cut(s) 275, 347, 433, 926, 1368
AfeI AGCGCT 1 cut(s) 1255
AfiI CCNNNNNNNGG 6 cut(s) 151, 317, 464, 992, 1209, 1303
AflII CTTAAG 1 cut(s) 637
AflIII ACRYGT 3 cut(s) 653, 1263, 1271
AgsI TTSAA 2 cut(s) 848, 946
AhdI GACNNNNNGTC 1 cut(s) 623
AjiI CACGTC 1 cut(s) 498
AjnI CCWGG 1 cut(s) 146
Alw21I GWGCWC 1 cut(s) 1221
Alw26I GTCTC 1 cut(s) 1178
AlwI GGATC 2 cut(s) 482, 530
AlwNI CAGNNNCTG 1 cut(s) 84
Ama87I CYCGRG 1 cut(s) 1019
Aor13HI TCCGGA 1 cut(s) 449
Aor51HI AGCGCT 1 cut(s) 1255
AoxI GGCC 1 cut(s) 886
ApeKI GCWGC 5 cut(s) 81, 84, 365, 716, 1145
ApoI RAATTY 1 cut(s) 1151
ArsI GACNNNNNNTTYG 6 cut(s) 53, 85, 228, 260, 738, 770
AspLEI GCGC 6 cut(s) 330, 902, 953, 1034, 1047, 1256
AspS9I GGNCC 4 cut(s) 253, 741, 983, 1246
AsuC2I CCSGG 4 cut(s) 392, 459, 1083, 1111
AsuHPI GGTGA 3 cut(s) 93, 390, 469
AvaI CYCGRG 1 cut(s) 1019
AvaII GGWCC 4 cut(s) 253, 741, 983, 1246
BaeGI GKGCMC 2 cut(s) 535, 1331
BalI TGGCCA 1 cut(s) 888
BanI GGYRCC 4 cut(s) 532, 644, 899, 1031
BanII GRGCYC 2 cut(s) 1111, 1221
BauI CACGAG 1 cut(s) 1301
Bbv12I GWGCWC 1 cut(s) 1221
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 5 cut(s) 68, 71, 377, 703, 1132
BccI CCATC 2 cut(s) 262, 887
BceAI ACGGC 2 cut(s) 364, 871
BcgI CGANNNNNNTGC 2 cut(s) 340, 374
BciT130I CCWGG 1 cut(s) 148
BciVI GTATCC 2 cut(s) 432, 563
BclI TGATCA 2 cut(s) 15, 75
BcnI CCSGG 4 cut(s) 392, 459, 1083, 1111
BcoDI GTCTC 1 cut(s) 1178
BfaI CTAG 1 cut(s) 797
BfoI RGCGCY 3 cut(s) 903, 1035, 1257
BfrI CTTAAG 1 cut(s) 637
BfuAI ACCTGC 2 cut(s) 422, 519
BfuI GTATCC 2 cut(s) 432, 563
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 8 cut(s) 82, 85, 366, 717, 866, 903, 921, 1146
BlpI GCTNAGC 1 cut(s) 869
BlsI GCNGC 8 cut(s) 83, 86, 367, 718, 867, 904, 922, 1147
Bme1390I CCNGG 5 cut(s) 148, 392, 459, 1083, 1111
Bme18I GGWCC 4 cut(s) 253, 741, 983, 1246
BmeRI GACNNNNNGTC 1 cut(s) 623
BmeT110I CYCGRG 1 cut(s) 1019
BmgBI CACGTC 1 cut(s) 498
BmgT120I GGNCC 4 cut(s) 253, 741, 983, 1246
BmiI GGNNCC 6 cut(s) 534, 646, 742, 790, 901, 1033
BmrFI CCNGG 5 cut(s) 148, 392, 459, 1083, 1111
BmrI ACTGGG 3 cut(s) 164, 170, 301
BmsI GCATC 1 cut(s) 352
BmuI ACTGGG 3 cut(s) 164, 170, 301
Bpu10I CCTNAGC 2 cut(s) 735, 1250
Bpu1102I GCTNAGC 1 cut(s) 869
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 4 cut(s) 392, 459, 1083, 1111
BsaAI YACGTR 3 cut(s) 491, 574, 824
BsaHI GRCGYC 2 cut(s) 900, 1032
BsaJI CCNNGG 6 cut(s) 146, 277, 458, 895, 963, 1203
BsaWI WCCGGW 3 cut(s) 342, 449, 471
BsaXI ACNNNNNCTCC 2 cut(s) 1175, 1205
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 317, 464, 992, 1209, 1303
Bse1I ACTGG 6 cut(s) 104, 170, 176, 296, 318, 889
Bse3DI GCAATG 1 cut(s) 630
BseAI TCCGGA 1 cut(s) 449
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 6 cut(s) 146, 277, 458, 895, 963, 1203
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 6 cut(s) 151, 317, 464, 992, 1209, 1303
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 5 cut(s) 726, 860, 1234, 1240, 1241
BseNI ACTGG 6 cut(s) 104, 170, 176, 296, 318, 889
BseRI GAGGAG 2 cut(s) 744, 1196
BseSI GKGCMC 2 cut(s) 535, 1331
BseXI GCAGC 5 cut(s) 68, 71, 377, 703, 1132
BseYI CCCAGC 2 cut(s) 117, 540
BsgI GTGCAG 2 cut(s) 25, 70
Bsh1236I CGCG 2 cut(s) 621, 951
Bsh1285I CGRYCG 1 cut(s) 251
BshFI GGCC 1 cut(s) 888
BshNI GGYRCC 4 cut(s) 532, 644, 899, 1031
BsiEI CGRYCG 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 1221
BsiHKCI CYCGRG 1 cut(s) 1019
BsiSI CCGG 8 cut(s) 343, 392, 450, 458, 472, 1082, 1111, 1244
BsiWI CGTACG 1 cut(s) 924
BslFI GGGAC 4 cut(s) 158, 475, 785, 1113
BslI CCNNNNNNNGG 6 cut(s) 151, 317, 464, 992, 1209, 1303
BsmAI GTCTC 1 cut(s) 1178
BsmFI GGGAC 4 cut(s) 158, 475, 785, 1113
BsnI GGCC 1 cut(s) 888
BsoBI CYCGRG 1 cut(s) 1019
Bsp1286I GDGCHC 4 cut(s) 535, 1111, 1221, 1331
Bsp13I TCCGGA 1 cut(s) 449
Bsp143I GATC 6 cut(s) 15, 75, 181, 474, 522, 1279
Bsp1720I GCTNAGC 1 cut(s) 869
BspANI GGCC 1 cut(s) 888
BspCNI CTCAG 5 cut(s) 727, 861, 1233, 1239, 1242
BspEI TCCGGA 1 cut(s) 449
BspFNI CGCG 2 cut(s) 621, 951
BspLI GGNNCC 6 cut(s) 534, 646, 742, 790, 901, 1033
BspMI ACCTGC 2 cut(s) 422, 519
BspPI GGATC 2 cut(s) 482, 530
BspT107I GGYRCC 4 cut(s) 532, 644, 899, 1031
BspTI CTTAAG 1 cut(s) 637
BsrDI GCAATG 1 cut(s) 630
BsrI ACTGG 6 cut(s) 104, 170, 176, 296, 318, 889
BssECI CCNNGG 6 cut(s) 146, 277, 458, 895, 963, 1203
BssMI GATC 6 cut(s) 15, 75, 181, 474, 522, 1279
BssNI GRCGYC 2 cut(s) 900, 1032
BssSI CACGAG 1 cut(s) 1301
BssT1I CCWWGG 1 cut(s) 277
Bst2BI CACGAG 1 cut(s) 1301
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 4 cut(s) 436, 967, 1001, 1191
BstACI GRCGYC 2 cut(s) 900, 1032
BstAFI CTTAAG 1 cut(s) 637
BstBAI YACGTR 3 cut(s) 491, 574, 824
BstC8I GCNNGC 1 cut(s) 809
BstDEI CTNAG 5 cut(s) 735, 869, 1220, 1226, 1250
BstDSI CCRYGG 1 cut(s) 963
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 2 cut(s) 621, 951
BstH2I RGCGCY 3 cut(s) 903, 1035, 1257
BstHHI GCGC 6 cut(s) 330, 902, 953, 1034, 1047, 1256
BstKTI GATC 6 cut(s) 18, 78, 184, 477, 525, 1282
BstMAI GTCTC 1 cut(s) 1178
BstMBI GATC 6 cut(s) 15, 75, 181, 474, 522, 1279
BstMCI CGRYCG 1 cut(s) 251
BstMWI GCNNNNNNNGC 8 cut(s) 118, 374, 546, 908, 917, 959, 1002, 1326
BstNI CCWGG 1 cut(s) 148
BstNSI RCATGY 3 cut(s) 657, 1267, 1275
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 5 cut(s) 146, 390, 457, 1081, 1109
BstSLI GKGCMC 2 cut(s) 535, 1331
BstUI CGCG 2 cut(s) 621, 951
BstV1I GCAGC 5 cut(s) 68, 71, 377, 703, 1132
BstXI CCANNNNNNTGG 1 cut(s) 658
BsuI GTATCC 2 cut(s) 432, 563
BsuRI GGCC 1 cut(s) 888
BtgI CCRYGG 1 cut(s) 963
BtgZI GCGATG 1 cut(s) 636
BtrI CACGTC 1 cut(s) 498
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 4 cut(s) 132, 311, 882, 1196
BveI ACCTGC 2 cut(s) 422, 519
Cac8I GCNNGC 1 cut(s) 809
CaiI CAGNNNCTG 1 cut(s) 84
CfoI GCGC 6 cut(s) 330, 902, 953, 1034, 1047, 1256
Cfr13I GGNCC 4 cut(s) 253, 741, 983, 1246
CpoI CGGWCCG 1 cut(s) 253
CseI GACGC 1 cut(s) 627
Csp6I GTAC 5 cut(s) 274, 346, 432, 925, 1367
CspI CGGWCCG 1 cut(s) 253
CviAII CATG 4 cut(s) 13, 654, 1264, 1272
CviQI GTAC 5 cut(s) 274, 346, 432, 925, 1367
DdeI CTNAG 5 cut(s) 735, 869, 1220, 1226, 1250
DinI GGCGCC 2 cut(s) 901, 1033
DpnI GATC 6 cut(s) 17, 77, 183, 476, 524, 1281
DpnII GATC 6 cut(s) 15, 75, 181, 474, 522, 1279
DraIII CACNNNGTG 1 cut(s) 1303
DriI GACNNNNNGTC 1 cut(s) 623
EaeI YGGCCR 1 cut(s) 886
Eam1105I GACNNNNNGTC 1 cut(s) 623
EciI GGCGGA 1 cut(s) 998
Ecl136II GAGCTC 1 cut(s) 1219
Eco130I CCWWGG 1 cut(s) 277
Eco24I GRGCYC 2 cut(s) 1111, 1221
Eco47I GGWCC 4 cut(s) 253, 741, 983, 1246
Eco47III AGCGCT 1 cut(s) 1255
Eco53kI GAGCTC 1 cut(s) 1219
Eco88I CYCGRG 1 cut(s) 1019
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 1219
EcoO65I GGTNACC 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 1151
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 277
EcoT38I GRGCYC 2 cut(s) 1111, 1221
EgeI GGCGCC 2 cut(s) 901, 1033
EheI GGCGCC 2 cut(s) 901, 1033
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 4 cut(s) 16, 657, 1267, 1275
FaiI YATR 9 cut(s) 14, 26, 406, 655, 971, 1142, 1259, 1265, 1273
FaqI GGGAC 4 cut(s) 158, 475, 785, 1113
FatI CATG 4 cut(s) 12, 653, 1263, 1271
FauI CCCGC 2 cut(s) 1114, 1167
FbaI TGATCA 2 cut(s) 15, 75
FblI GTMKAC 1 cut(s) 1095
Fnu4HI GCNGC 8 cut(s) 82, 85, 366, 717, 866, 903, 921, 1146
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 2 cut(s) 1111, 1221
Fsp4HI GCNGC 8 cut(s) 82, 85, 366, 717, 866, 903, 921, 1146
FspBI CTAG 1 cut(s) 797
FspI TGCGCA 2 cut(s) 329, 1046
GlaI GCGC 6 cut(s) 329, 901, 952, 1033, 1046, 1255
GluI GCNGC 8 cut(s) 82, 85, 366, 717, 866, 903, 921, 1146
GsaI CCCAGC 2 cut(s) 121, 544
HaeII RGCGCY 3 cut(s) 903, 1035, 1257
HaeIII GGCC 1 cut(s) 888
HapII CCGG 8 cut(s) 343, 392, 450, 458, 472, 1082, 1111, 1244
HgaI GACGC 1 cut(s) 627
HhaI GCGC 6 cut(s) 330, 902, 953, 1034, 1047, 1256
Hin1I GRCGYC 2 cut(s) 900, 1032
Hin1II CATG 4 cut(s) 16, 657, 1267, 1275
Hin6I GCGC 6 cut(s) 328, 900, 951, 1032, 1045, 1254
HinP1I GCGC 6 cut(s) 328, 900, 951, 1032, 1045, 1254
HincII GTYRAC 2 cut(s) 1096, 1269
HindII GTYRAC 2 cut(s) 1096, 1269
HindIII AAGCTT 1 cut(s) 805
HinfI GANTC 7 cut(s) 282, 395, 484, 681, 1126, 1165, 1224
HpaII CCGG 8 cut(s) 343, 392, 450, 458, 472, 1082, 1111, 1244
HphI GGTGA 3 cut(s) 93, 390, 469
Hpy166II GTNNAC 5 cut(s) 132, 1054, 1096, 1269, 1369
Hpy188I TCNGA 7 cut(s) 257, 680, 745, 1170, 1204, 1223, 1286
Hpy188III TCNNGA 8 cut(s) 286, 450, 757, 786, 1019, 1021, 1156, 1277
Hpy8I GTNNAC 5 cut(s) 132, 1054, 1096, 1269, 1369
Hpy99I CGWCG 4 cut(s) 362, 621, 863, 1100
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 4 cut(s) 436, 967, 1001, 1191
HpyCH4IV ACGT 5 cut(s) 490, 497, 573, 823, 1116
HpyCH4V TGCA 6 cut(s) 42, 87, 365, 417, 811, 1335
HpyF10VI GCNNNNNNNGC 8 cut(s) 118, 374, 546, 908, 917, 959, 1002, 1326
HpyF3I CTNAG 5 cut(s) 735, 869, 1220, 1226, 1250
HpySE526I ACGT 5 cut(s) 490, 497, 573, 823, 1116
Hsp92I GRCGYC 2 cut(s) 900, 1032
Hsp92II CATG 4 cut(s) 16, 657, 1267, 1275
HspAI GCGC 6 cut(s) 328, 900, 951, 1032, 1045, 1254
KasI GGCGCC 2 cut(s) 899, 1031
Kpn2I TCCGGA 1 cut(s) 449
Ksp22I TGATCA 2 cut(s) 15, 75
Kzo9I GATC 6 cut(s) 15, 75, 181, 474, 522, 1279
LmnI GCTCC 1 cut(s) 731
Lsp1109I GCAGC 5 cut(s) 68, 71, 377, 703, 1132
LweI GCATC 1 cut(s) 352
MaeI CTAG 1 cut(s) 797
MaeII ACGT 5 cut(s) 490, 497, 573, 823, 1116
MaeIII GTNAC 3 cut(s) 467, 682, 1274
MalI GATC 6 cut(s) 17, 77, 183, 476, 524, 1281
MboI GATC 6 cut(s) 15, 75, 181, 474, 522, 1279
MboII GAAGA 2 cut(s) 839, 1073
MfeI CAATTG 1 cut(s) 158
MhlI GDGCHC 4 cut(s) 535, 1111, 1221, 1331
MlsI TGGCCA 1 cut(s) 888
MluCI AATT 7 cut(s) 158, 262, 336, 352, 831, 1151, 1159
MluNI TGGCCA 1 cut(s) 888
Mly113I GGCGCC 2 cut(s) 900, 1032
MlyI GAGTC 4 cut(s) 291, 690, 1135, 1218
Mox20I TGGCCA 1 cut(s) 888
MroI TCCGGA 1 cut(s) 449
MscI TGGCCA 1 cut(s) 888
MseI TTAA 2 cut(s) 507, 638
Msp20I TGGCCA 1 cut(s) 888
MspA1I CMGCKG 3 cut(s) 81, 561, 868
MspCI CTTAAG 1 cut(s) 637
MspI CCGG 8 cut(s) 343, 392, 450, 458, 472, 1082, 1111, 1244
MspR9I CCNGG 5 cut(s) 148, 392, 459, 1083, 1111
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 1 cut(s) 148
MvnI CGCG 2 cut(s) 621, 951
MwoI GCNNNNNNNGC 8 cut(s) 118, 374, 546, 908, 917, 959, 1002, 1326
NarI GGCGCC 2 cut(s) 900, 1032
NciI CCSGG 4 cut(s) 392, 459, 1083, 1111
NdeII GATC 6 cut(s) 15, 75, 181, 474, 522, 1279
NlaIII CATG 4 cut(s) 16, 657, 1267, 1275
NlaIV GGNNCC 6 cut(s) 534, 646, 742, 790, 901, 1033
NmeAIII GCCGAG 1 cut(s) 876
NmuCI GTSAC 2 cut(s) 682, 1274
NsbI TGCGCA 2 cut(s) 329, 1046
NspI RCATGY 3 cut(s) 657, 1267, 1275
PaeR7I CTCGAG 1 cut(s) 1019
PciI ACATGT 3 cut(s) 653, 1263, 1271
PcsI WCGNNNNNNNCGW 3 cut(s) 253, 354, 1095
PfeI GAWTC 3 cut(s) 395, 484, 1165
Pfl23II CGTACG 1 cut(s) 924
PflFI GACNNNGTC 1 cut(s) 797
PflMI CCANNNNNTGG 2 cut(s) 992, 1303
PkrI GCNGC 8 cut(s) 83, 86, 367, 718, 867, 904, 922, 1147
PleI GAGTC 4 cut(s) 290, 689, 1134, 1218
PluTI GGCGCC 2 cut(s) 903, 1035
PpsI GAGTC 4 cut(s) 290, 689, 1134, 1218
Ppu21I YACGTR 3 cut(s) 491, 574, 824
PscI ACATGT 3 cut(s) 653, 1263, 1271
Psp124BI GAGCTC 1 cut(s) 1221
Psp6I CCWGG 1 cut(s) 146
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 2 cut(s) 117, 540
PspGI CCWGG 1 cut(s) 146
PspLI CGTACG 1 cut(s) 924
PspN4I GGNNCC 6 cut(s) 534, 646, 742, 790, 901, 1033
PspPI GGNCC 4 cut(s) 253, 741, 983, 1246
PstNI CAGNNNCTG 1 cut(s) 84
PsyI GACNNNGTC 1 cut(s) 797
PvuII CAGCTG 2 cut(s) 81, 561
RsaI GTAC 5 cut(s) 275, 347, 433, 926, 1368
RsaNI GTAC 5 cut(s) 274, 346, 432, 925, 1367
Rsr2I CGGWCCG 1 cut(s) 253
RsrII CGGWCCG 1 cut(s) 253
SacI GAGCTC 1 cut(s) 1221
SalI GTCGAC 1 cut(s) 1094
SaqAI TTAA 2 cut(s) 507, 638
SatI GCNGC 8 cut(s) 82, 85, 366, 717, 866, 903, 921, 1146
Sau3AI GATC 6 cut(s) 15, 75, 181, 474, 522, 1279
Sau96I GGNCC 4 cut(s) 253, 741, 983, 1246
SchI GAGTC 4 cut(s) 291, 690, 1135, 1218
ScrFI CCNGG 5 cut(s) 148, 392, 459, 1083, 1111
SduI GDGCHC 4 cut(s) 535, 1111, 1221, 1331
SfaNI GCATC 1 cut(s) 352
SfoI GGCGCC 2 cut(s) 901, 1033
Sfr274I CTCGAG 1 cut(s) 1019
SinI GGWCC 4 cut(s) 253, 741, 983, 1246
SlaI CTCGAG 1 cut(s) 1019
SmlI CTYRAG 3 cut(s) 65, 637, 1019
SmoI CTYRAG 3 cut(s) 65, 637, 1019
Sse9I AATT 7 cut(s) 158, 262, 336, 352, 831, 1151, 1159
SspDI GGCGCC 2 cut(s) 899, 1031
SspMI CTAG 1 cut(s) 797
SstI GAGCTC 1 cut(s) 1221
StyD4I CCNGG 5 cut(s) 146, 390, 457, 1081, 1109
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 4 cut(s) 436, 967, 1001, 1191
TaiI ACGT 5 cut(s) 493, 500, 576, 826, 1119
TaqI TCGA 8 cut(s) 306, 360, 525, 669, 756, 1020, 1095, 1179
TaqII GACCGA 2 cut(s) 237, 241
TasI AATT 7 cut(s) 158, 262, 336, 352, 831, 1151, 1159
TatI WGTACW 1 cut(s) 1366
TauI GCSGC 3 cut(s) 868, 905, 923
TfiI GAWTC 3 cut(s) 395, 484, 1165
Tru1I TTAA 2 cut(s) 507, 638
Tru9I TTAA 2 cut(s) 507, 638
TscAI CASTG 4 cut(s) 139, 318, 889, 1196
TseFI GTSAC 2 cut(s) 682, 1274
TseI GCWGC 5 cut(s) 81, 84, 365, 716, 1145
Tsp45I GTSAC 2 cut(s) 682, 1274
TspDTI ATGAA 3 cut(s) 17, 612, 1274
TspGWI ACGGA 2 cut(s) 333, 1127
TspRI CASTG 4 cut(s) 139, 318, 889, 1196
Tth111I GACNNNGTC 1 cut(s) 797
Van91I CCANNNNNTGG 2 cut(s) 992, 1303
Vha464I CTTAAG 1 cut(s) 637
VpaK11BI GGWCC 4 cut(s) 253, 741, 983, 1246
XapI RAATTY 1 cut(s) 1151
XceI RCATGY 3 cut(s) 657, 1267, 1275
XhoI CTCGAG 1 cut(s) 1019
XmiI GTMKAC 1 cut(s) 1095
XspI CTAG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.