MD02G1213100.v1.1

Phospholipase A(1) DAD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014224
Physical Location & Seq
Reverse (-)
23064872 .. 23066236
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1213100.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGAAGCTTACTATCAGACCCTCCTCACTAGTCCCTACTCCAAAACACAAGGCAACATTCACCTGCTACACCCTCACTCAATTACCAATTGTAAACCCCAATCAGATGACCTTCACTCACAACCTTCCCAACTGGCAACAATTGCTCAATCCAGTTGACATTAGATCCCTAGCACTCACCCCTATCACCTCCATAACCACCACCACCACAACATCTACTGCTGCCTCCTCCGCCGCAGCTGCCAAACAACCCATTCGCAAAAATTGGATGGAGTACCAAGGCTTCAACAACTGGGAAGGTCTTCTCGACCCTCTAGACGCCAATTTACGTGGCGAGATTATACGGTATGGACAATTTGTCGATGCTGCATACAAGGCGTTTGACTTTGATCCGGACTCTAATTCATACGCAACATGCAAGTACTCGAAAGCCTCCTTGTTTGAAAACTCCGGGCTTCCGGGAACTGGCTACCGGATTACAAAAAATTTACGTGCCACGTCCGGCATCAAGCTTCCTAGCTGGATAGAAAAGGCTCCGAGTTGGGTCGCGACACAGTCCAGCTGGATTGGCTACATGGCGGTATGTCAAGACAAGGAGGAAATTGCAAGGCTTGGAAGACGGGACGTTGTGATTGCCTTTAGAGGTACAGCAACGTGCTTGGAGTGGCTCGAAAATCTTCGAGCAACTCTCACCCAACTCCCGTCCGTACTTAATAACTCAGGCCTGGACAACTGTGAACCAATGGTCGAAAGTGGATTTTTGAGCTTATACACTTCAGGAACCGACTTAATCCCGAGCTTGCAACAAATGGTACGCCAAGAGATTGCACGGTTGCTTCAATCCTACGGCGACGAGCCTCTCAGCATAACATTCACTGGGCACAGCCTTGGCGCCGCGCTAGCAACGCTCGCAGCGTGCGACATTAAAACTACGTTTAACCCGTCACCACTCGTCACCGTCATGTCGTTTGCCGGGCCACGTGTCGGAAACAGTAGCTTCCGCCGCAACCTGGATAAGAAGGGCGCAAAAGTTTTACGCATTGTGAACTCCGACGATGTAATAACTAAAATACCGGGTTTCGTAATCGACGACAATGAAAGGCGCCACAAGGCGTGCGATGGTGGGTTCCGAAAGGCGGTAGAGTTTCAGAACTGGATCCAGAAGAAGGTGGAGGACACGCAGTTGTCGTACGCGGAGGTTGGGAAGGAGCTCAGGTTAAGTAGCAAGGACTCGCCGTATCTGATGGGCACCAACGTGTGCACGTGTCACGAGCTCAGCACGTACCTCCACCTTGTCGACAATTTCCAGAGCTCAAAGTGTCCGTTCAAAGCCACGGCCAAGAGATTATTCAGCAAAAAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

455

Amino Acids

50.45

Weight (kDa)

9.02

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 210 - 360 2.3e-36 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44810 AT2G44810
fragaria_vesca FvH4_7g10210
malus_domestica MD02G1213100.v1.1 MD07G1109300.v1.1
prunus_persica Prupe.2G132000_v2.0.a1 Prupe.2G132100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0347941
rosa_laevigata RLG00000028702
rosa_multiflora Rmu_ssc0000342.1_g000017
rosa_roxburghii Rroxscaffold_4G00307360
rosa_rugosa Rorug01G0190400
rosa_samantha Rh1AG209000 Rh1BG173600 Rh1CG193200 Rh1DG203600
rosa_wichuraiana Rw1G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 71
AccB1I GGYRCC 3 cut(s) 890, 1101, 1247
AccI GTMKAC 1 cut(s) 1296
AccII CGCG 3 cut(s) 548, 896, 1193
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 8 cut(s) 231, 234, 578, 894, 1000, 1003, 1136, 1193
AclWI GGATC 4 cut(s) 159, 383, 1150, 1163
AcoI YGGCCR 1 cut(s) 1335
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 1 cut(s) 759
AcvI CACGTG 2 cut(s) 980, 1263
AcyI GRCGYC 3 cut(s) 318, 891, 1102
AfaI GTAC 7 cut(s) 275, 422, 646, 708, 813, 1190, 1283
AfiI CCNNNNNNNGG 5 cut(s) 464, 983, 1009, 1135, 1165
AflIII ACRYGT 3 cut(s) 979, 1254, 1262
AgsI TTSAA 4 cut(s) 286, 443, 839, 1327
AhdI GACNNNNNGTC 1 cut(s) 356
AhlI ACTAGT 1 cut(s) 28
AjiI CACGTC 1 cut(s) 498
AjnI CCWGG 2 cut(s) 723, 1008
AjuI GAANNNNNNNTTGG 2 cut(s) 1331, 1363
AleI CACNNNNGTG 1 cut(s) 1253
Alw21I GWGCWC 4 cut(s) 1212, 1262, 1275, 1313
Alw44I GTGCAC 1 cut(s) 1258
AlwI GGATC 4 cut(s) 159, 383, 1150, 1163
Ama87I CYCGRG 1 cut(s) 793
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 3 cut(s) 721, 974, 1335
ApaLI GTGCAC 1 cut(s) 1258
ApeKI GCWGC 5 cut(s) 221, 236, 239, 365, 911
ApoI RAATTY 1 cut(s) 484
Asp700I GAANNNNTTC 2 cut(s) 300, 675
AspLEI GCGC 4 cut(s) 893, 898, 1025, 1104
AspS9I GGNCC 1 cut(s) 974
AsuC2I CCSGG 4 cut(s) 451, 459, 973, 1074
AsuHPI GGTGA 6 cut(s) 52, 169, 178, 682, 936, 946
AsuNHI GCTAGC 1 cut(s) 898
AvaI CYCGRG 1 cut(s) 793
BaeGI GKGCMC 3 cut(s) 882, 1250, 1262
BamHI GGATCC 1 cut(s) 1155
BanI GGYRCC 3 cut(s) 890, 1101, 1247
BanII GRGCYC 3 cut(s) 1212, 1275, 1313
BarI GAAGNNNNNNTAC 2 cut(s) 266, 298
BauI CACGAG 1 cut(s) 1268
BbrPI CACGTG 2 cut(s) 980, 1263
BbsI GAAGAC 2 cut(s) 293, 622
Bbv12I GWGCWC 4 cut(s) 1212, 1262, 1275, 1313
BbvI GCAGC 5 cut(s) 208, 226, 248, 352, 923
BccI CCATC 3 cut(s) 262, 1112, 1237
BceAI ACGGC 3 cut(s) 862, 1219, 1350
BciT130I CCWGG 2 cut(s) 725, 1010
BcnI CCSGG 4 cut(s) 451, 459, 973, 1074
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 5 cut(s) 29, 170, 314, 516, 899
BfoI RGCGCY 2 cut(s) 894, 1105
BfuAI ACCTGC 1 cut(s) 71
BisI GCNGC 8 cut(s) 222, 234, 237, 240, 366, 894, 912, 1003
BlpI GCTNAGC 1 cut(s) 1274
BlsI GCNGC 8 cut(s) 223, 235, 238, 241, 367, 895, 913, 1004
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 6 cut(s) 451, 459, 725, 973, 1010, 1074
BmeRI GACNNNNNGTC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 793
BmgBI CACGTC 1 cut(s) 498
BmgT120I GGNCC 1 cut(s) 974
BmiI GGNNCC 7 cut(s) 534, 781, 892, 1103, 1127, 1157, 1249
BmrFI CCNGG 6 cut(s) 451, 459, 725, 973, 1010, 1074
BmrI ACTGGG 2 cut(s) 301, 885
BmsI GCATC 2 cut(s) 352, 513
BmtI GCTAGC 1 cut(s) 902
BmuI ACTGGG 2 cut(s) 301, 885
BpiI GAAGAC 2 cut(s) 293, 622
BplI GAGNNNNNCTC 2 cut(s) 672, 704
Bpu10I CCTNAGC 1 cut(s) 1211
Bpu1102I GCTNAGC 1 cut(s) 1274
BpuMI CCSGG 4 cut(s) 451, 459, 973, 1074
BsaAI YACGTR 5 cut(s) 329, 491, 980, 1263, 1281
BsaHI GRCGYC 3 cut(s) 318, 891, 1102
BsaJI CCNNGG 3 cut(s) 277, 886, 1332
BsaWI WCCGGW 2 cut(s) 391, 471
BsaXI ACNNNNNCTCC 2 cut(s) 1199, 1229
Bsc4I CCNNNNNNNGG 5 cut(s) 464, 983, 1009, 1135, 1165
Bse1I ACTGG 6 cut(s) 137, 152, 296, 469, 880, 1157
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 2 cut(s) 725, 1010
BseDI CCNNGG 3 cut(s) 277, 886, 1332
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 5 cut(s) 464, 983, 1009, 1135, 1165
BseMII CTCAG 4 cut(s) 732, 874, 1225, 1288
BseNI ACTGG 6 cut(s) 137, 152, 296, 469, 880, 1157
BseRI GAGGAG 2 cut(s) 13, 217
BseSI GKGCMC 3 cut(s) 882, 1250, 1262
BseXI GCAGC 5 cut(s) 208, 226, 248, 352, 923
Bsh1236I CGCG 3 cut(s) 548, 896, 1193
BshFI GGCC 3 cut(s) 723, 976, 1337
BshNI GGYRCC 3 cut(s) 890, 1101, 1247
BsiHKAI GWGCWC 4 cut(s) 1212, 1262, 1275, 1313
BsiHKCI CYCGRG 1 cut(s) 793
BsiSI CCGG 7 cut(s) 392, 450, 458, 472, 501, 972, 1073
BsiWI CGTACG 1 cut(s) 1188
BslFI GGGAC 2 cut(s) 17, 635
BslI CCNNNNNNNGG 5 cut(s) 464, 983, 1009, 1135, 1165
BsmFI GGGAC 2 cut(s) 17, 635
BsnI GGCC 3 cut(s) 723, 976, 1337
BsoBI CYCGRG 1 cut(s) 793
Bsp1286I GDGCHC 6 cut(s) 882, 1212, 1250, 1262, 1275, 1313
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 3 cut(s) 164, 388, 1155
Bsp1720I GCTNAGC 1 cut(s) 1274
Bsp68I TCGCGA 1 cut(s) 548
BspACI CCGC 8 cut(s) 231, 234, 578, 894, 1000, 1003, 1136, 1193
BspANI GGCC 3 cut(s) 723, 976, 1337
BspCNI CTCAG 4 cut(s) 731, 873, 1224, 1287
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 3 cut(s) 548, 896, 1193
BspLI GGNNCC 7 cut(s) 534, 781, 892, 1103, 1127, 1157, 1249
BspMI ACCTGC 1 cut(s) 71
BspOI GCTAGC 1 cut(s) 902
BspPI GGATC 4 cut(s) 159, 383, 1150, 1163
BspT107I GGYRCC 3 cut(s) 890, 1101, 1247
BsrI ACTGG 6 cut(s) 137, 152, 296, 469, 880, 1157
BssECI CCNNGG 3 cut(s) 277, 886, 1332
BssMI GATC 3 cut(s) 164, 388, 1155
BssNI GRCGYC 3 cut(s) 318, 891, 1102
BssSI CACGAG 1 cut(s) 1268
BssT1I CCWWGG 2 cut(s) 277, 886
Bst2BI CACGAG 1 cut(s) 1268
Bst2UI CCWGG 2 cut(s) 725, 1010
Bst4CI ACNGT 6 cut(s) 345, 555, 734, 831, 958, 992
BstACI GRCGYC 3 cut(s) 318, 891, 1102
BstAPI GCANNNNNTGC 1 cut(s) 142
BstBAI YACGTR 5 cut(s) 329, 491, 980, 1263, 1281
BstC8I GCNNGC 5 cut(s) 800, 900, 909, 916, 1114
BstDEI CTNAG 4 cut(s) 718, 860, 1211, 1274
BstDSI CCRYGG 1 cut(s) 1332
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 3 cut(s) 548, 896, 1193
BstH2I RGCGCY 2 cut(s) 894, 1105
BstHHI GCGC 4 cut(s) 893, 898, 1025, 1104
BstKTI GATC 3 cut(s) 167, 391, 1158
BstMBI GATC 3 cut(s) 164, 388, 1155
BstMWI GCNNNNNNNGC 9 cut(s) 142, 230, 239, 374, 567, 899, 904, 908, 1002
BstNI CCWGG 2 cut(s) 725, 1010
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 6 cut(s) 449, 457, 723, 971, 1008, 1072
BstSLI GKGCMC 3 cut(s) 882, 1250, 1262
BstUI CGCG 3 cut(s) 548, 896, 1193
BstV1I GCAGC 5 cut(s) 208, 226, 248, 352, 923
BstV2I GAAGAC 2 cut(s) 293, 622
BstX2I RGATCY 2 cut(s) 164, 1155
BstYI RGATCY 2 cut(s) 164, 1155
BsuRI GGCC 3 cut(s) 723, 976, 1337
BtgI CCRYGG 1 cut(s) 1332
BtgZI GCGATG 1 cut(s) 1131
BtrI CACGTC 1 cut(s) 498
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 873
BtuMI TCGCGA 1 cut(s) 548
BveI ACCTGC 1 cut(s) 71
Cac8I GCNNGC 5 cut(s) 800, 900, 909, 916, 1114
CfoI GCGC 4 cut(s) 893, 898, 1025, 1104
Cfr13I GGNCC 1 cut(s) 974
CseI GACGC 1 cut(s) 326
Csp6I GTAC 7 cut(s) 274, 421, 645, 707, 812, 1189, 1282
CspCI CAANNNNNGTGG 2 cut(s) 310, 345
CviAII CATG 3 cut(s) 414, 574, 961
CviQI GTAC 7 cut(s) 274, 421, 645, 707, 812, 1189, 1282
DdeI CTNAG 4 cut(s) 718, 860, 1211, 1274
DinI GGCGCC 2 cut(s) 892, 1103
DpnI GATC 3 cut(s) 166, 390, 1157
DpnII GATC 3 cut(s) 164, 388, 1155
DriI GACNNNNNGTC 1 cut(s) 356
EaeI YGGCCR 1 cut(s) 1335
Eam1105I GACNNNNNGTC 1 cut(s) 356
EciI GGCGGA 2 cut(s) 220, 989
Ecl136II GAGCTC 3 cut(s) 1210, 1273, 1311
Eco130I CCWWGG 2 cut(s) 277, 886
Eco147I AGGCCT 1 cut(s) 723
Eco24I GRGCYC 3 cut(s) 1212, 1275, 1313
Eco53kI GAGCTC 3 cut(s) 1210, 1273, 1311
Eco57I CTGAAG 1 cut(s) 759
Eco72I CACGTG 2 cut(s) 980, 1263
Eco88I CYCGRG 1 cut(s) 793
EcoICRI GAGCTC 3 cut(s) 1210, 1273, 1311
EcoRII CCWGG 2 cut(s) 723, 1008
EcoT14I CCWWGG 2 cut(s) 277, 886
EcoT38I GRGCYC 3 cut(s) 1212, 1275, 1313
EgeI GGCGCC 2 cut(s) 892, 1103
EheI GGCGCC 2 cut(s) 892, 1103
ErhI CCWWGG 2 cut(s) 277, 886
FaeI CATG 3 cut(s) 417, 577, 964
FaqI GGGAC 2 cut(s) 17, 635
FatI CATG 3 cut(s) 413, 573, 960
FblI GTMKAC 1 cut(s) 1296
Fnu4HI GCNGC 8 cut(s) 222, 234, 237, 240, 366, 894, 912, 1003
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 3 cut(s) 1212, 1275, 1313
Fsp4HI GCNGC 8 cut(s) 222, 234, 237, 240, 366, 894, 912, 1003
FspBI CTAG 5 cut(s) 29, 170, 314, 516, 899
GlaI GCGC 4 cut(s) 892, 897, 1024, 1103
GluI GCNGC 8 cut(s) 222, 234, 237, 240, 366, 894, 912, 1003
HaeII RGCGCY 2 cut(s) 894, 1105
HaeIII GGCC 3 cut(s) 723, 976, 1337
HapII CCGG 7 cut(s) 392, 450, 458, 472, 501, 972, 1073
HgaI GACGC 1 cut(s) 326
HhaI GCGC 4 cut(s) 893, 898, 1025, 1104
Hin1I GRCGYC 3 cut(s) 318, 891, 1102
Hin1II CATG 3 cut(s) 417, 577, 964
Hin6I GCGC 4 cut(s) 891, 896, 1023, 1102
HinP1I GCGC 4 cut(s) 891, 896, 1023, 1102
HincII GTYRAC 2 cut(s) 157, 1297
HindII GTYRAC 2 cut(s) 157, 1297
HindIII AAGCTT 2 cut(s) 5, 509
HinfI GANTC 2 cut(s) 395, 1229
HpaII CCGG 7 cut(s) 392, 450, 458, 472, 501, 972, 1073
HphI GGTGA 6 cut(s) 52, 169, 178, 682, 936, 946
Hpy166II GTNNAC 6 cut(s) 94, 157, 737, 1045, 1260, 1297
Hpy188I TCNGA 8 cut(s) 17, 105, 537, 986, 1051, 1130, 1149, 1242
Hpy8I GTNNAC 6 cut(s) 94, 157, 737, 1045, 1260, 1297
Hpy99I CGWCG 3 cut(s) 854, 1055, 1091
HpyAV CCTTC 6 cut(s) 121, 134, 290, 1012, 1159, 1198
HpyCH4III ACNGT 6 cut(s) 345, 555, 734, 831, 958, 992
HpyCH4V TGCA 6 cut(s) 368, 417, 605, 802, 827, 1260
HpyF10VI GCNNNNNNNGC 9 cut(s) 142, 230, 239, 374, 567, 899, 904, 908, 1002
HpyF3I CTNAG 4 cut(s) 718, 860, 1211, 1274
Hsp92I GRCGYC 3 cut(s) 318, 891, 1102
Hsp92II CATG 3 cut(s) 417, 577, 964
HspAI GCGC 4 cut(s) 891, 896, 1023, 1102
KasI GGCGCC 2 cut(s) 890, 1101
Kpn2I TCCGGA 1 cut(s) 391
Kzo9I GATC 3 cut(s) 164, 388, 1155
LmnI GCTCC 2 cut(s) 538, 1207
Lsp1109I GCAGC 5 cut(s) 208, 226, 248, 352, 923
LweI GCATC 2 cut(s) 352, 513
MaeI CTAG 5 cut(s) 29, 170, 314, 516, 899
MaeIII GTNAC 3 cut(s) 942, 952, 1265
MalI GATC 3 cut(s) 166, 390, 1157
MboI GATC 3 cut(s) 164, 388, 1155
MboII GAAGA 4 cut(s) 293, 627, 668, 1174
MfeI CAATTG 2 cut(s) 87, 140
MflI RGATCY 2 cut(s) 164, 1155
MhlI GDGCHC 6 cut(s) 882, 1212, 1250, 1262, 1275, 1313
Mly113I GGCGCC 2 cut(s) 891, 1102
MlyI GAGTC 2 cut(s) 389, 1223
MmeI TCCRAC 2 cut(s) 964, 1074
MroI TCCGGA 1 cut(s) 391
MroXI GAANNNNTTC 2 cut(s) 300, 675
MseI TTAA 5 cut(s) 711, 788, 924, 936, 1217
MslI CAYNNNNRTG 2 cut(s) 959, 1253
MspA1I CMGCKG 2 cut(s) 239, 561
MspI CCGG 7 cut(s) 392, 450, 458, 472, 501, 972, 1073
MspR9I CCNGG 6 cut(s) 451, 459, 725, 973, 1010, 1074
MteI GCGCNGCGC 1 cut(s) 894
MunI CAATTG 2 cut(s) 87, 140
MvaI CCWGG 2 cut(s) 725, 1010
MvnI CGCG 3 cut(s) 548, 896, 1193
MwoI GCNNNNNNNGC 9 cut(s) 142, 230, 239, 374, 567, 899, 904, 908, 1002
NarI GGCGCC 2 cut(s) 891, 1102
NciI CCSGG 4 cut(s) 451, 459, 973, 1074
NdeII GATC 3 cut(s) 164, 388, 1155
NheI GCTAGC 1 cut(s) 898
NlaIII CATG 3 cut(s) 417, 577, 964
NlaIV GGNNCC 7 cut(s) 534, 781, 892, 1103, 1127, 1157, 1249
NmuCI GTSAC 3 cut(s) 942, 952, 1265
NruI TCGCGA 1 cut(s) 548
NspI RCATGY 1 cut(s) 417
OliI CACNNNNGTG 1 cut(s) 1253
PaqCI CACCTGC 1 cut(s) 71
PceI AGGCCT 1 cut(s) 723
PcsI WCGNNNNNNNCGW 5 cut(s) 911, 915, 938, 1086, 1184
PdmI GAANNNNTTC 2 cut(s) 300, 675
Pfl23II CGTACG 1 cut(s) 1188
PflFI GACNNNGTC 1 cut(s) 553
PfoI TCCNGGA 1 cut(s) 457
PkrI GCNGC 8 cut(s) 223, 235, 238, 241, 367, 895, 913, 1004
PleI GAGTC 2 cut(s) 389, 1223
PluTI GGCGCC 2 cut(s) 894, 1105
PmaCI CACGTG 2 cut(s) 980, 1263
PmlI CACGTG 2 cut(s) 980, 1263
PpsI GAGTC 2 cut(s) 389, 1223
Ppu21I YACGTR 5 cut(s) 329, 491, 980, 1263, 1281
Psp124BI GAGCTC 3 cut(s) 1212, 1275, 1313
Psp6I CCWGG 2 cut(s) 723, 1008
PspCI CACGTG 2 cut(s) 980, 1263
PspGI CCWGG 2 cut(s) 723, 1008
PspLI CGTACG 1 cut(s) 1188
PspN4I GGNNCC 7 cut(s) 534, 781, 892, 1103, 1127, 1157, 1249
PspPI GGNCC 1 cut(s) 974
PsuI RGATCY 2 cut(s) 164, 1155
PsyI GACNNNGTC 1 cut(s) 553
PvuII CAGCTG 2 cut(s) 239, 561
RruI TCGCGA 1 cut(s) 548
RsaI GTAC 7 cut(s) 275, 422, 646, 708, 813, 1190, 1283
RsaNI GTAC 7 cut(s) 274, 421, 645, 707, 812, 1189, 1282
RseI CAYNNNNRTG 2 cut(s) 959, 1253
SacI GAGCTC 3 cut(s) 1212, 1275, 1313
SalI GTCGAC 1 cut(s) 1295
SaqAI TTAA 5 cut(s) 711, 788, 924, 936, 1217
SatI GCNGC 8 cut(s) 222, 234, 237, 240, 366, 894, 912, 1003
Sau3AI GATC 3 cut(s) 164, 388, 1155
Sau96I GGNCC 1 cut(s) 974
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 2 cut(s) 389, 1223
ScrFI CCNGG 6 cut(s) 451, 459, 725, 973, 1010, 1074
SduI GDGCHC 6 cut(s) 882, 1212, 1250, 1262, 1275, 1313
SfaNI GCATC 2 cut(s) 352, 513
SfoI GGCGCC 2 cut(s) 892, 1103
SmiMI CAYNNNNRTG 2 cut(s) 959, 1253
SpeI ACTAGT 1 cut(s) 28
SseBI AGGCCT 1 cut(s) 723
SsiI CCGC 8 cut(s) 231, 234, 578, 894, 1000, 1003, 1136, 1193
SspDI GGCGCC 2 cut(s) 890, 1101
SspMI CTAG 5 cut(s) 29, 170, 314, 516, 899
SstI GAGCTC 3 cut(s) 1212, 1275, 1313
StuI AGGCCT 1 cut(s) 723
StyD4I CCNGG 6 cut(s) 449, 457, 723, 971, 1008, 1072
StyI CCWWGG 2 cut(s) 277, 886
TaaI ACNGT 6 cut(s) 345, 555, 734, 831, 958, 992
TaqI TCGA 8 cut(s) 306, 360, 425, 669, 679, 747, 1086, 1296
TatI WGTACW 1 cut(s) 420
TauI GCSGC 3 cut(s) 236, 896, 1005
Tru1I TTAA 5 cut(s) 711, 788, 924, 936, 1217
Tru9I TTAA 5 cut(s) 711, 788, 924, 936, 1217
TscAI CASTG 1 cut(s) 880
TseFI GTSAC 3 cut(s) 942, 952, 1265
TseI GCWGC 5 cut(s) 221, 236, 239, 365, 911
Tsp45I GTSAC 3 cut(s) 942, 952, 1265
TspDTI ATGAA 3 cut(s) 17, 393, 1110
TspGWI ACGGA 2 cut(s) 694, 1311
TspRI CASTG 1 cut(s) 880
Tth111I GACNNNGTC 1 cut(s) 553
VneI GTGCAC 1 cut(s) 1258
XapI RAATTY 1 cut(s) 484
XbaI TCTAGA 1 cut(s) 313
XceI RCATGY 1 cut(s) 417
XmiI GTMKAC 1 cut(s) 1296
XmnI GAANNNNTTC 2 cut(s) 300, 675
XspI CTAG 5 cut(s) 29, 170, 314, 516, 899
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.