AT3G04360

CONTAINS InterPro DOMAIN s C2 membrane targeting protein (InterPro IPR018029), C2 calcium lipid-binding region, CaLB (InterPro IPR008973), C2 calcium-dependent membrane targeting (InterPro IPR000008)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011068
Physical Location & Seq
Reverse (-)
1156908 .. 1158470
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04360.1

Sequence Viewer

Length: 1176 bp
ATGGGTCGGCTCAAGTTCGCATGTCCGGTTCTTGAGATCAATTTAATCTCAGCTCAAGACTTAGCCCCTGTCTCAAGAAACATGAAAACGTATTCCGTCGCTTGGATCAACACCGATCCTATGAGAAAACTCACCACGCGTGTTGATCAATCCAACAGAGCTAACCCTATATGGAACGAGAAGTTTGTGTTTCGTGTCAACGACAAGATTCTCTACGTTGACGCATCAGCGATTGTGATTGAGATCTACGCAGCGGCTTGGGCTAAAGATGCTCTTGTTGGTACTGTGAATGTTCTTCTCAGCGATCTTTTTGCTCCTTGGTCTGGTTTTGGTGATGGTAACGACGGTGGTGGTGGGAATAACAACATGAGGCTTGTGACTCTTCAGATTCGTCGTCCTTCAGGGAGGCTACAAGGGTTTTTACGTCTCGGTGTTGCACTTCTTGACGGTGGTCAACGGAGTATGCCTTTATCTATTGAGGTTTTCGATGGAAGTCGAAAAGGAGAGAGGAATAGTGTTAAAAGAGACCAAGAGGCTTCAAGAATGATGCATCGCCGTACGAATAGCGATCAAACGGATTTAACCACGTCTACGAATGATTACGGTGTGAAAACAGGGGTTGTTACTGGCAATGGAGGAGGAGGTGGTGGTGGTGGCGGTATTGTTGTTGGTGCGGATAGTATGGTTAATGGTTCGCTTTGTAGCTCTGATATTGGACCATCAGCGTCTGTGGTTGCGGCTGCGATTGCGCAAGGGCTTTACAATAGACAGAAAACAGCGGTTAAAGCGGCTAACAAAGAGGACGCAAGTTCGATACTTGAAGGGAAAACAGAGGGAATTGAGCATAGAGTTGAACGGTGGAGAGCAGAGAAAAATGGCACGGGTGGAGCGGGTGGAGCGGCTGGATCAAGCGATGATTCTAGCGGGAAAGGAGGAGCGGGAAGGAGAAGAAGGAGGAGGAGAAGAAAGGAGAAACAACAAGGGAGGAGAAACGGTGGAGGAGAGGGTAAGAAAGGGTTGTTTTCATGTTTTGGAAACGTGTTTGGATGTGAGATTTCGATAACATGTGGCGGTGGAAGCGGCGGAGAAGGAGACAGCACAAAGAAGAGATATAATAACAATAAAGTAGTAAATCTTAGTGCTGTAGACGAAACGTTTAGTCATTCTGCGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

391

Amino Acids

41.46

Weight (kDa)

9.78

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 11 - 103 3e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13350 AT3G04360
fragaria_vesca FvH4_6g01610
malus_domestica MD00G1149000.v1.1 MD04G1232000.v1.1
prunus_persica Prupe.6G351400_v2.0.a1
pyrus_communis pycom04g20540 pycom12g22960
rosa_chinensis RchiOBHm_Chr3g0449421
rosa_laevigata RLG00000025832
rosa_multiflora Rmu_sc0019012.1_g000008 Rmu_sc0019478.1_g000001 Rmu_sc0020017.1_g000003
rosa_roxburghii Rroxscaffold_6G00427380
rosa_rugosa Rorug02G0620500
rosa_samantha Rh3AG018300 Rh3BG018600 Rh3CG017500 Rh3DG019000
rosa_wichuraiana Rw3G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 750
AccBSI CCGCTC 3 cut(s) 890, 899, 938
AccI GTMKAC 2 cut(s) 590, 1146
AccII CGCG 1 cut(s) 139
AclI AACGTT 1 cut(s) 1154
AclWI GGATC 3 cut(s) 110, 113, 913
AcuI CTGAAG 2 cut(s) 368, 384
AfaI GTAC 2 cut(s) 283, 559
AfiI CCNNNNNNNGG 2 cut(s) 102, 323
AflIII ACRYGT 3 cut(s) 137, 1038, 1064
AgsI TTSAA 3 cut(s) 540, 821, 854
AjiI CACGTC 1 cut(s) 588
AluBI AGCT 3 cut(s) 53, 161, 705
AluI AGCT 3 cut(s) 53, 161, 705
Alw26I GTCTC 4 cut(s) 76, 431, 519, 1086
AlwI GGATC 3 cut(s) 110, 113, 913
AlwNI CAGNNNCTG 1 cut(s) 728
ApeKI GCWGC 2 cut(s) 251, 740
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 716
AsuHPI GGTGA 2 cut(s) 124, 344
AvaII GGWCC 1 cut(s) 716
BbvI GCAGC 2 cut(s) 263, 727
BccI CCATC 3 cut(s) 329, 482, 727
BceAI ACGGC 1 cut(s) 540
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 4 cut(s) 76, 431, 519, 1086
BfaI CTAG 1 cut(s) 921
BfmI CTRYAG 1 cut(s) 1143
BglII AGATCT 1 cut(s) 243
BisI GCNGC 7 cut(s) 252, 255, 738, 741, 789, 900, 1081
BlsI GCNGC 7 cut(s) 253, 256, 739, 742, 790, 901, 1082
Bme18I GGWCC 1 cut(s) 716
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 716
BmsI GCATC 4 cut(s) 233, 259, 537, 559
BoxI GACNNNNGTC 1 cut(s) 450
BpuEI CTTGAG 3 cut(s) 39, 53, 58
BsaBI GATNNNNATC 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 519
BsaJI CCNNGG 1 cut(s) 317
BsaWI WCCGGW 1 cut(s) 25
BsaXI ACNNNNNCTCC 2 cut(s) 979, 1009
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 323
Bse1I ACTGG 1 cut(s) 631
Bse3DI GCAATG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 317
BseGI GGATG 1 cut(s) 1052
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 102, 323
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 2 cut(s) 63, 313
BseNI ACTGG 1 cut(s) 631
BseRI GAGGAG 7 cut(s) 651, 654, 948, 970, 973, 1000, 1014
BseXI GCAGC 2 cut(s) 263, 727
Bsh1236I CGCG 1 cut(s) 139
BsiSI CCGG 1 cut(s) 26
BsiWI CGTACG 1 cut(s) 557
BslI CCNNNNNNNGG 2 cut(s) 102, 323
BsmAI GTCTC 4 cut(s) 76, 431, 519, 1086
BsmBI CGTCTC 1 cut(s) 431
Bso31I GGTCTC 1 cut(s) 519
Bsp143I GATC 8 cut(s) 36, 105, 115, 145, 243, 304, 568, 905
BspCNI CTCAG 2 cut(s) 62, 312
BspFNI CGCG 1 cut(s) 139
BspPI GGATC 3 cut(s) 110, 113, 913
BspTNI GGTCTC 1 cut(s) 519
BsrBI CCGCTC 3 cut(s) 890, 899, 938
BsrDI GCAATG 1 cut(s) 637
BsrI ACTGG 1 cut(s) 631
BssECI CCNNGG 1 cut(s) 317
BssMI GATC 8 cut(s) 36, 105, 115, 145, 243, 304, 568, 905
BssT1I CCWWGG 1 cut(s) 317
Bst4CI ACNGT 6 cut(s) 286, 347, 449, 605, 858, 995
Bst6I CTCTTC 2 cut(s) 387, 1100
BstDEI CTNAG 4 cut(s) 49, 61, 299, 1136
BstF5I GGATG 1 cut(s) 1052
BstFNI CGCG 1 cut(s) 139
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 8 cut(s) 39, 108, 118, 148, 246, 307, 571, 908
BstMAI GTCTC 4 cut(s) 76, 431, 519, 1086
BstMBI GATC 8 cut(s) 36, 105, 115, 145, 243, 304, 568, 905
BstMWI GCNNNNNNNGC 6 cut(s) 260, 269, 746, 785, 896, 1077
BstNSI RCATGY 2 cut(s) 24, 1068
BstPAI GACNNNNGTC 1 cut(s) 450
BstSFI CTRYAG 1 cut(s) 1143
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 2 cut(s) 263, 727
BstX2I RGATCY 1 cut(s) 243
BstYI RGATCY 1 cut(s) 243
BtgZI GCGATG 2 cut(s) 536, 927
BtrI CACGTC 1 cut(s) 588
BtsCI GGATG 1 cut(s) 1052
CaiI CAGNNNCTG 1 cut(s) 728
CfoI GCGC 1 cut(s) 751
Cfr13I GGNCC 1 cut(s) 716
CseI GACGC 3 cut(s) 230, 714, 812
Csp6I GTAC 2 cut(s) 282, 558
CspCI CAANNNNNGTGG 2 cut(s) 124, 159
CviAII CATG 5 cut(s) 21, 82, 367, 1026, 1065
CviQI GTAC 2 cut(s) 282, 558
DdeI CTNAG 4 cut(s) 49, 61, 299, 1136
DpnI GATC 8 cut(s) 38, 107, 117, 147, 245, 306, 570, 907
DpnII GATC 8 cut(s) 36, 105, 115, 145, 243, 304, 568, 905
Eam1104I CTCTTC 2 cut(s) 387, 1100
EarI CTCTTC 2 cut(s) 387, 1100
EciI GGCGGA 1 cut(s) 1098
Eco130I CCWWGG 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 519
Eco47I GGWCC 1 cut(s) 716
Eco57I CTGAAG 2 cut(s) 368, 384
EcoT14I CCWWGG 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 317
Esp3I CGTCTC 1 cut(s) 431
FaeI CATG 5 cut(s) 24, 85, 370, 1029, 1068
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FatI CATG 5 cut(s) 20, 81, 366, 1025, 1064
FauI CCCGC 3 cut(s) 883, 917, 931
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 2 cut(s) 590, 1146
Fnu4HI GCNGC 7 cut(s) 252, 255, 738, 741, 789, 900, 1081
FokI GGATG 1 cut(s) 1059
Fsp4HI GCNGC 7 cut(s) 252, 255, 738, 741, 789, 900, 1081
FspBI CTAG 1 cut(s) 921
FspI TGCGCA 1 cut(s) 750
GlaI GCGC 1 cut(s) 750
GluI GCNGC 7 cut(s) 252, 255, 738, 741, 789, 900, 1081
HapII CCGG 1 cut(s) 26
HgaI GACGC 3 cut(s) 230, 714, 812
HhaI GCGC 1 cut(s) 751
Hin1II CATG 5 cut(s) 24, 85, 370, 1029, 1068
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HincII GTYRAC 3 cut(s) 199, 220, 455
HindII GTYRAC 3 cut(s) 199, 220, 455
HinfI GANTC 4 cut(s) 208, 379, 388, 917
HpaII CCGG 1 cut(s) 26
HphI GGTGA 2 cut(s) 124, 344
Hpy166II GTNNAC 5 cut(s) 199, 220, 455, 591, 1147
Hpy188I TCNGA 2 cut(s) 387, 709
Hpy188III TCNNGA 5 cut(s) 32, 56, 75, 443, 540
Hpy8I GTNNAC 5 cut(s) 199, 220, 455, 591, 1147
Hpy99I CGWCG 3 cut(s) 101, 347, 396
HpyAV CCTTC 5 cut(s) 408, 815, 936, 945, 1082
HpyCH4III ACNGT 6 cut(s) 286, 347, 449, 605, 858, 995
HpyCH4IV ACGT 6 cut(s) 89, 216, 424, 587, 1038, 1154
HpyCH4V TGCA 2 cut(s) 437, 550
HpyF10VI GCNNNNNNNGC 6 cut(s) 260, 269, 746, 785, 896, 1077
HpyF3I CTNAG 4 cut(s) 49, 61, 299, 1136
HpySE526I ACGT 6 cut(s) 89, 216, 424, 587, 1038, 1154
Hsp92II CATG 5 cut(s) 24, 85, 370, 1029, 1068
HspAI GCGC 1 cut(s) 749
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 8 cut(s) 36, 105, 115, 145, 243, 304, 568, 905
LmnI GCTCC 4 cut(s) 319, 887, 896, 935
LpnPI CCDG 7 cut(s) 39, 81, 309, 387, 600, 612, 888
Lsp1109I GCAGC 2 cut(s) 263, 727
LweI GCATC 4 cut(s) 233, 259, 537, 559
MaeI CTAG 1 cut(s) 921
MaeII ACGT 6 cut(s) 89, 216, 424, 587, 1038, 1154
MaeIII GTNAC 3 cut(s) 338, 376, 622
MalI GATC 8 cut(s) 38, 107, 117, 147, 245, 306, 570, 907
MbiI CCGCTC 3 cut(s) 890, 899, 938
MboI GATC 8 cut(s) 36, 105, 115, 145, 243, 304, 568, 905
MboII GAAGA 5 cut(s) 287, 374, 960, 975, 1117
MflI RGATCY 1 cut(s) 243
MluCI AATT 2 cut(s) 40, 837
MluI ACGCGT 1 cut(s) 137
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 1 cut(s) 177
Mph1103I ATGCAT 1 cut(s) 552
MseI TTAA 5 cut(s) 44, 519, 581, 687, 783
MspA1I CMGCKG 2 cut(s) 254, 779
MspI CCGG 1 cut(s) 26
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 6 cut(s) 260, 269, 746, 785, 896, 1077
NdeII GATC 8 cut(s) 36, 105, 115, 145, 243, 304, 568, 905
NlaIII CATG 5 cut(s) 24, 85, 370, 1029, 1068
NmuCI GTSAC 1 cut(s) 376
NsbI TGCGCA 1 cut(s) 750
NsiI ATGCAT 1 cut(s) 552
NspI RCATGY 2 cut(s) 24, 1068
PciI ACATGT 1 cut(s) 1064
PfeI GAWTC 3 cut(s) 208, 388, 917
Pfl23II CGTACG 1 cut(s) 557
PkrI GCNGC 7 cut(s) 253, 256, 739, 742, 790, 901, 1082
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
PscI ACATGT 1 cut(s) 1064
PshAI GACNNNNGTC 1 cut(s) 450
Psp1406I AACGTT 1 cut(s) 1154
PspLI CGTACG 1 cut(s) 557
PspPI GGNCC 1 cut(s) 716
PstNI CAGNNNCTG 1 cut(s) 728
PsuI RGATCY 1 cut(s) 243
RsaI GTAC 2 cut(s) 283, 559
RsaNI GTAC 2 cut(s) 282, 558
SaqAI TTAA 5 cut(s) 44, 519, 581, 687, 783
SatI GCNGC 7 cut(s) 252, 255, 738, 741, 789, 900, 1081
Sau3AI GATC 8 cut(s) 36, 105, 115, 145, 243, 304, 568, 905
Sau96I GGNCC 1 cut(s) 716
SchI GAGTC 1 cut(s) 373
SfaNI GCATC 4 cut(s) 233, 259, 537, 559
SfcI CTRYAG 1 cut(s) 1143
SinI GGWCC 1 cut(s) 716
SmlI CTYRAG 4 cut(s) 11, 32, 54, 73
SmoI CTYRAG 4 cut(s) 11, 32, 54, 73
Sse9I AATT 2 cut(s) 40, 837
SspMI CTAG 1 cut(s) 921
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 6 cut(s) 286, 347, 449, 605, 858, 995
TaiI ACGT 6 cut(s) 92, 219, 427, 590, 1041, 1157
TaqI TCGA 4 cut(s) 486, 496, 812, 1058
TasI AATT 2 cut(s) 40, 837
TauI GCSGC 5 cut(s) 257, 740, 791, 902, 1083
TfiI GAWTC 3 cut(s) 208, 388, 917
Tru1I TTAA 5 cut(s) 44, 519, 581, 687, 783
Tru9I TTAA 5 cut(s) 44, 519, 581, 687, 783
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 2 cut(s) 251, 740
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 2 cut(s) 98, 1014
TspGWI ACGGA 3 cut(s) 85, 472, 590
VpaK11BI GGWCC 1 cut(s) 716
XceI RCATGY 2 cut(s) 24, 1068
XmiI GTMKAC 2 cut(s) 590, 1146
XspI CTAG 1 cut(s) 921
Zsp2I ATGCAT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.