pycom04g20540

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011068
Physical Location & Seq
Reverse (-)
22017925 .. 22019133
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20540.1

Sequence Viewer

Length: 1209 bp
ATGCCACGGCCGTTCGGCTCCTCCTTCCAGCTGCTGGAGATCAACCTGATCTCGGCGCAGGACCTGACGCCGGCGTCGAAGGCGATGCGGACGTTCGCCGTCGCGTGGGTCAACCCCAAGCGGAAGCTGACGACGCGTTTCGACCAGACCGGCCACACCAACCCGACGTGGAACGAGAAGTTCGTCTTCCGAGTTGACGACGACTTCCTCCGCGACGACACGTCGAAGATCATGATCGAGATCTACGCCTCCGCATGGCTCCGAGACGTCCTGATCGGAACCGCCGCGGTCGTGGTGAACAATTTGCAGAACAAGTCCAAGATGCGGTTCATGGCGATCCAGCTCCGCCGCCCGTCCGGCCGCCCGCAGGGGATTCTGAACATCGGGCTCGGCCTCCTCGACAGCACAATGCGGAGCATGCCGCTCTACAGCGAGCTCAGCTCCTCCGCCGTCGGGTACTGGGATCTGATGGAGGGAAAAACCGGTAACCAGAAGAGCAGCGGCCTCAATTCCAAGGATCAAGACAAATTCGTCATCTTCCAACGATCTCAGAGCGATCGGACGGGTAGCGATTCCGGATTCTCGAAATCCAGGCCCGCGTCCTCCATCTGCGATGGCGTCACAAAAATCAGGCCGTCGAACTCCACCTGCAATGCCTCCGCCTACAAGCCCAAAACTCAAGCCTCCTTCTGCAACAGCTCGATTGTGAACGGATCTGAGCTCAGCACAGAGCAAAAGAAAGGAAACGTGAACGGGTCCCTCTGCTCTGACGTTGGTCCTTCGCCCTCCGTGGTTGCGGCCGCAATCGCCAAGGGAATCTACCCGTTGCCGTACACCGGAGCCCGAGCGCCGTCTCCCGATGATGCCGGAAGCTCGTTGCTGGACGAGTGGGCGGACCAGGACAGCGTGGAAGGGTTAAAGACCAAGATCGAACGGTGGCGGACGGAGCTGCCGCCGGTGTACGACTGCGCCAAGAACAACGCAAAGAGTTCCCATATTCAGAAGCCACTCCAATCGTCCCACCGCCGTCCCAGCAGTCAGAAGACGCGGCGGAGCCGCAGCAGCGGCAAAGGATTGTTCTCCTGCTTCGCGTTGGGTTGCGAGATTTCGATCTCTTGCGGCAGCAAACCGAAGATTAACAAGAAGAGCGGGAAAGTGCACCTCAGCCCGTCAGATTTGACTTTCGACGACGACTCGTACTTACGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

403

Amino Acids

44.03

Weight (kDa)

9.54

Isoelectric Point (pI)

56.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 11 - 107 1.3e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13350 AT3G04360
fragaria_vesca FvH4_6g01610
malus_domestica MD00G1149000.v1.1 MD04G1232000.v1.1
prunus_persica Prupe.6G351400_v2.0.a1
pyrus_communis pycom04g20540 pycom12g22960
rosa_chinensis RchiOBHm_Chr3g0449421
rosa_laevigata RLG00000025832
rosa_multiflora Rmu_sc0019012.1_g000008 Rmu_sc0019478.1_g000001 Rmu_sc0020017.1_g000003
rosa_roxburghii Rroxscaffold_6G00427380
rosa_rugosa Rorug02G0620500
rosa_samantha Rh3AG018300 Rh3BG018600 Rh3CG017500 Rh3DG019000
rosa_wichuraiana Rw3G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 656
AasI GACNNNNNNGTC 2 cut(s) 73, 530
AatII GACGTC 1 cut(s) 270
Acc36I ACCTGC 1 cut(s) 656
AccB7I CCANNNNNTGG 1 cut(s) 34
AccBSI CCGCTC 2 cut(s) 424, 1149
AccII CGCG 7 cut(s) 104, 136, 213, 287, 599, 1048, 1091
AccIII TCCGGA 1 cut(s) 575
AclWI GGATC 4 cut(s) 331, 471, 525, 721
AcoI YGGCCR 4 cut(s) 8, 151, 358, 798
AcsI RAATTY 1 cut(s) 527
AcyI GRCGYC 4 cut(s) 68, 74, 267, 618
AfaI GTAC 4 cut(s) 458, 833, 962, 1199
AfiI CCNNNNNNNGG 9 cut(s) 34, 52, 70, 105, 255, 324, 367, 453, 836
AflIII ACRYGT 2 cut(s) 134, 219
AgeI ACCGGT 1 cut(s) 482
AhdI GACNNNNNGTC 1 cut(s) 220
AjiI CACGTC 2 cut(s) 168, 222
AjnI CCWGG 2 cut(s) 590, 897
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 9 cut(s) 31, 127, 343, 436, 441, 699, 721, 873, 949
AluI AGCT 9 cut(s) 31, 127, 343, 436, 441, 699, 721, 873, 949
Alw21I GWGCWC 3 cut(s) 438, 723, 1161
Alw26I GTCTC 2 cut(s) 258, 858
Alw44I GTGCAC 1 cut(s) 1157
AlwI GGATC 4 cut(s) 331, 471, 525, 721
AlwNI CAGNNNCTG 2 cut(s) 34, 64
Ama87I CYCGRG 1 cut(s) 843
Aor13HI TCCGGA 1 cut(s) 575
AoxI GGCC 8 cut(s) 8, 151, 358, 391, 502, 593, 632, 798
ApaLI GTGCAC 1 cut(s) 1157
ApeKI GCWGC 6 cut(s) 31, 498, 949, 1059, 1062, 1122
ApoI RAATTY 1 cut(s) 527
AsiGI ACCGGT 1 cut(s) 482
AspLEI GCGC 3 cut(s) 58, 850, 971
AspS9I GGNCC 5 cut(s) 61, 594, 756, 776, 895
AsuHPI GGTGA 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 843
AvaII GGWCC 4 cut(s) 61, 756, 776, 895
BaeGI GKGCMC 1 cut(s) 1161
BanII GRGCYC 4 cut(s) 390, 438, 723, 844
BbsI GAAGAC 2 cut(s) 178, 1049
Bbv12I GWGCWC 3 cut(s) 438, 723, 1161
BbvCI CCTCAGC 1 cut(s) 1163
BbvI GCAGC 6 cut(s) 18, 510, 936, 1071, 1074, 1134
BccI CCATC 3 cut(s) 463, 608, 614
BceAI ACGGC 7 cut(s) 23, 83, 434, 619, 814, 835, 1011
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 592, 899
BcoDI GTCTC 2 cut(s) 258, 858
BfmI CTRYAG 1 cut(s) 427
BfoI RGCGCY 1 cut(s) 851
BfuAI ACCTGC 1 cut(s) 656
BglI GCCNNNNNGGC 1 cut(s) 357
BglII AGATCT 1 cut(s) 240
BlpI GCTNAGC 2 cut(s) 437, 722
Bme1390I CCNGG 2 cut(s) 592, 899
Bme18I GGWCC 4 cut(s) 61, 756, 776, 895
BmeRI GACNNNNNGTC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 843
BmgBI CACGTC 2 cut(s) 168, 222
BmgT120I GGNCC 5 cut(s) 61, 594, 756, 776, 895
BmiI GGNNCC 7 cut(s) 19, 260, 280, 757, 758, 841, 1055
BmrFI CCNGG 2 cut(s) 592, 899
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 3 cut(s) 75, 312, 853
BmuI ACTGGG 1 cut(s) 469
BoxI GACNNNNGTC 1 cut(s) 774
BpiI GAAGAC 2 cut(s) 178, 1049
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BpmI CTGGAG 1 cut(s) 56
Bpu10I CCTNAGC 1 cut(s) 1163
Bpu1102I GCTNAGC 2 cut(s) 437, 722
BpuEI CTTGAG 1 cut(s) 663
BsaBI GATNNNNATC 3 cut(s) 233, 239, 1109
BsaHI GRCGYC 4 cut(s) 68, 74, 267, 618
BsaJI CCNNGG 5 cut(s) 5, 285, 513, 789, 810
BsaWI WCCGGW 3 cut(s) 482, 575, 836
Bsc4I CCNNNNNNNGG 9 cut(s) 34, 52, 70, 105, 255, 324, 367, 453, 836
Bse118I RCCGGY 4 cut(s) 70, 149, 482, 955
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 1 cut(s) 658
Bse8I GATNNNNATC 3 cut(s) 233, 239, 1109
BseAI TCCGGA 1 cut(s) 575
BseBI CCWGG 2 cut(s) 592, 899
BseDI CCNNGG 5 cut(s) 5, 285, 513, 789, 810
BseJI GATNNNNATC 3 cut(s) 233, 239, 1109
BseLI CCNNNNNNNGG 9 cut(s) 34, 52, 70, 105, 255, 324, 367, 453, 836
BseMI GCAATG 1 cut(s) 658
BseMII CTCAG 5 cut(s) 451, 563, 708, 736, 1177
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 3 cut(s) 10, 386, 433
BseSI GKGCMC 1 cut(s) 1161
BseX3I CGGCCG 3 cut(s) 8, 358, 798
BseXI GCAGC 6 cut(s) 18, 510, 936, 1071, 1074, 1134
BseYI CCCAGC 1 cut(s) 1031
Bsh1236I CGCG 7 cut(s) 104, 136, 213, 287, 599, 1048, 1091
Bsh1285I CGRYCG 5 cut(s) 11, 291, 361, 559, 801
BshFI GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
BshTI ACCGGT 1 cut(s) 482
BsiEI CGRYCG 5 cut(s) 11, 291, 361, 559, 801
BsiHKAI GWGCWC 3 cut(s) 438, 723, 1161
BsiHKCI CYCGRG 1 cut(s) 843
BsiSI CCGG 8 cut(s) 71, 150, 357, 483, 576, 837, 867, 956
BslFI GGGAC 3 cut(s) 742, 1003, 1014
BslI CCNNNNNNNGG 9 cut(s) 34, 52, 70, 105, 255, 324, 367, 453, 836
BsmAI GTCTC 2 cut(s) 258, 858
BsmBI CGTCTC 2 cut(s) 258, 858
BsmFI GGGAC 3 cut(s) 742, 1003, 1014
BsnI GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
BsoBI CYCGRG 1 cut(s) 843
Bsp1286I GDGCHC 5 cut(s) 390, 438, 723, 844, 1161
Bsp13I TCCGGA 1 cut(s) 575
Bsp1720I GCTNAGC 2 cut(s) 437, 722
BspANI GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
BspCNI CTCAG 5 cut(s) 450, 562, 709, 735, 1176
BspEI TCCGGA 1 cut(s) 575
BspFNI CGCG 7 cut(s) 104, 136, 213, 287, 599, 1048, 1091
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 7 cut(s) 19, 260, 280, 757, 758, 841, 1055
BspMI ACCTGC 1 cut(s) 656
BspPI GGATC 4 cut(s) 331, 471, 525, 721
BspQI GCTCTTC 2 cut(s) 488, 1139
BsrBI CCGCTC 2 cut(s) 424, 1149
BsrDI GCAATG 1 cut(s) 658
BsrFI RCCGGY 4 cut(s) 70, 149, 482, 955
BsrI ACTGG 1 cut(s) 464
BssAI RCCGGY 4 cut(s) 70, 149, 482, 955
BssECI CCNNGG 5 cut(s) 5, 285, 513, 789, 810
BssNI GRCGYC 4 cut(s) 68, 74, 267, 618
BssT1I CCWWGG 2 cut(s) 513, 810
Bst2UI CCWGG 2 cut(s) 592, 899
Bst4CI ACNGT 2 cut(s) 936, 1206
Bst6I CTCTTC 2 cut(s) 488, 1139
BstACI GRCGYC 4 cut(s) 68, 74, 267, 618
BstC8I GCNNGC 5 cut(s) 72, 365, 419, 434, 597
BstDEI CTNAG 5 cut(s) 437, 549, 717, 722, 1163
BstDSI CCRYGG 3 cut(s) 5, 285, 789
BstEII GGTNACC 1 cut(s) 485
BstFNI CGCG 7 cut(s) 104, 136, 213, 287, 599, 1048, 1091
BstH2I RGCGCY 1 cut(s) 851
BstHHI GCGC 3 cut(s) 58, 850, 971
BstMAI GTCTC 2 cut(s) 258, 858
BstMCI CGRYCG 5 cut(s) 11, 291, 361, 559, 801
BstNI CCWGG 2 cut(s) 592, 899
BstNSI RCATGY 1 cut(s) 421
BstPAI GACNNNNGTC 1 cut(s) 774
BstPI GGTNACC 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 590, 897
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 1161
BstUI CGCG 7 cut(s) 104, 136, 213, 287, 599, 1048, 1091
BstV1I GCAGC 6 cut(s) 18, 510, 936, 1071, 1074, 1134
BstV2I GAAGAC 2 cut(s) 178, 1049
BstX2I RGATCY 3 cut(s) 240, 463, 713
BstYI RGATCY 3 cut(s) 240, 463, 713
BstZI CGGCCG 3 cut(s) 8, 358, 798
BsuRI GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
BtgI CCRYGG 3 cut(s) 5, 285, 789
BtgZI GCGATG 2 cut(s) 98, 627
BtrI CACGTC 2 cut(s) 168, 222
BveI ACCTGC 1 cut(s) 656
Cac8I GCNNGC 5 cut(s) 72, 365, 419, 434, 597
CaiI CAGNNNCTG 2 cut(s) 34, 64
CciI TCATGA 1 cut(s) 231
CciNI GCGGCCGC 1 cut(s) 798
CfoI GCGC 3 cut(s) 58, 850, 971
Cfr10I RCCGGY 4 cut(s) 70, 149, 482, 955
Cfr13I GGNCC 5 cut(s) 61, 594, 756, 776, 895
Cfr42I CCGCGG 1 cut(s) 288
CseI GACGC 6 cut(s) 63, 76, 142, 588, 607, 1054
Csp6I GTAC 4 cut(s) 457, 832, 961, 1198
CspAI ACCGGT 1 cut(s) 482
CviAII CATG 4 cut(s) 232, 255, 331, 418
CviQI GTAC 4 cut(s) 457, 832, 961, 1198
DdeI CTNAG 5 cut(s) 437, 549, 717, 722, 1163
DrdI GACNNNNNNGTC 2 cut(s) 73, 530
DriI GACNNNNNGTC 1 cut(s) 220
DseDI GACNNNNNNGTC 2 cut(s) 73, 530
EaeI YGGCCR 4 cut(s) 8, 151, 358, 798
EagI CGGCCG 3 cut(s) 8, 358, 798
Eam1104I CTCTTC 2 cut(s) 488, 1139
Eam1105I GACNNNNNGTC 1 cut(s) 220
EarI CTCTTC 2 cut(s) 488, 1139
EciI GGCGGA 6 cut(s) 335, 436, 649, 908, 955, 1066
Ecl136II GAGCTC 2 cut(s) 436, 721
EclXI CGGCCG 3 cut(s) 8, 358, 798
Eco130I CCWWGG 2 cut(s) 513, 810
Eco24I GRGCYC 4 cut(s) 390, 438, 723, 844
Eco47I GGWCC 4 cut(s) 61, 756, 776, 895
Eco52I CGGCCG 3 cut(s) 8, 358, 798
Eco53kI GAGCTC 2 cut(s) 436, 721
Eco88I CYCGRG 1 cut(s) 843
Eco91I GGTNACC 1 cut(s) 485
EcoICRI GAGCTC 2 cut(s) 436, 721
EcoO109I RGGNCCY 2 cut(s) 61, 756
EcoO65I GGTNACC 1 cut(s) 485
EcoRII CCWGG 2 cut(s) 590, 897
EcoT14I CCWWGG 2 cut(s) 513, 810
EcoT38I GRGCYC 4 cut(s) 390, 438, 723, 844
ErhI CCWWGG 2 cut(s) 513, 810
Esp3I CGTCTC 2 cut(s) 258, 858
FaeI CATG 4 cut(s) 235, 258, 334, 421
FaiI YATR 5 cut(s) 233, 256, 332, 419, 996
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 3 cut(s) 742, 1003, 1014
FatI CATG 4 cut(s) 231, 254, 330, 417
FauI CCCGC 3 cut(s) 372, 604, 1142
FriOI GRGCYC 4 cut(s) 390, 438, 723, 844
GlaI GCGC 3 cut(s) 57, 849, 970
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 851
HaeIII GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
HapII CCGG 8 cut(s) 71, 150, 357, 483, 576, 837, 867, 956
HgaI GACGC 6 cut(s) 63, 76, 142, 588, 607, 1054
HhaI GCGC 3 cut(s) 58, 850, 971
Hin1I GRCGYC 4 cut(s) 68, 74, 267, 618
Hin1II CATG 4 cut(s) 235, 258, 334, 421
Hin6I GCGC 3 cut(s) 56, 848, 969
HinP1I GCGC 3 cut(s) 56, 848, 969
HincII GTYRAC 2 cut(s) 112, 196
HindII GTYRAC 2 cut(s) 112, 196
HinfI GANTC 5 cut(s) 373, 572, 579, 816, 1193
HpaII CCGG 8 cut(s) 71, 150, 357, 483, 576, 837, 867, 956
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 8 cut(s) 112, 196, 298, 709, 751, 834, 961, 1159
Hpy188III TCNNGA 7 cut(s) 232, 238, 271, 521, 576, 583, 857
Hpy8I GTNNAC 8 cut(s) 112, 196, 298, 709, 751, 834, 961, 1159
HpyAV CCTTC 5 cut(s) 34, 73, 697, 789, 905
HpyCH4III ACNGT 2 cut(s) 936, 1206
HpyCH4IV ACGT 6 cut(s) 92, 167, 221, 267, 747, 771
HpyCH4V TGCA 4 cut(s) 307, 651, 693, 1159
HpyF3I CTNAG 5 cut(s) 437, 549, 717, 722, 1163
HpySE526I ACGT 6 cut(s) 92, 167, 221, 267, 747, 771
Hsp92I GRCGYC 4 cut(s) 68, 74, 267, 618
Hsp92II CATG 4 cut(s) 235, 258, 334, 421
HspAI GCGC 3 cut(s) 56, 848, 969
KflI GGGWCCC 1 cut(s) 756
Kpn2I TCCGGA 1 cut(s) 575
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
KspI CCGCGG 1 cut(s) 288
LguI GCTCTTC 2 cut(s) 488, 1139
LmnI GCTCC 8 cut(s) 23, 264, 348, 414, 446, 839, 946, 1053
Lsp1109I GCAGC 6 cut(s) 18, 510, 936, 1071, 1074, 1134
LweI GCATC 3 cut(s) 75, 312, 853
MaeII ACGT 6 cut(s) 92, 167, 221, 267, 747, 771
MaeIII GTNAC 2 cut(s) 485, 619
MbiI CCGCTC 2 cut(s) 424, 1149
MboII GAAGA 7 cut(s) 178, 238, 505, 529, 1054, 1144, 1156
MflI RGATCY 3 cut(s) 240, 463, 713
MhlI GDGCHC 5 cut(s) 390, 438, 723, 844, 1161
MluCI AATT 3 cut(s) 301, 508, 527
MluI ACGCGT 1 cut(s) 134
MlyI GAGTC 1 cut(s) 1187
MmeI TCCRAC 1 cut(s) 565
MreI CGCCGGCG 1 cut(s) 70
MroI TCCGGA 1 cut(s) 575
MroNI GCCGGC 1 cut(s) 70
MseI TTAA 2 cut(s) 917, 1137
MspA1I CMGCKG 4 cut(s) 31, 287, 501, 1065
MspI CCGG 8 cut(s) 71, 150, 357, 483, 576, 837, 867, 956
MspR9I CCNGG 2 cut(s) 592, 899
MvaI CCWGG 2 cut(s) 592, 899
MvnI CGCG 7 cut(s) 104, 136, 213, 287, 599, 1048, 1091
NaeI GCCGGC 1 cut(s) 72
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 4 cut(s) 235, 258, 334, 421
NlaIV GGNNCC 7 cut(s) 19, 260, 280, 757, 758, 841, 1055
NmeAIII GCCGAG 2 cut(s) 32, 369
NmuCI GTSAC 1 cut(s) 619
NotI GCGGCCGC 1 cut(s) 798
NspI RCATGY 1 cut(s) 421
PaeI GCATGC 1 cut(s) 421
PagI TCATGA 1 cut(s) 231
PaqCI CACCTGC 1 cut(s) 656
PciSI GCTCTTC 2 cut(s) 488, 1139
PcsI WCGNNNNNNNCGW 3 cut(s) 74, 180, 396
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 4 cut(s) 373, 572, 579, 816
PflMI CCANNNNNTGG 1 cut(s) 34
PinAI ACCGGT 1 cut(s) 482
Ple19I CGATCG 1 cut(s) 559
PleI GAGTC 1 cut(s) 1187
PpsI GAGTC 1 cut(s) 1187
PpuMI RGGWCCY 2 cut(s) 61, 756
PshAI GACNNNNGTC 1 cut(s) 774
Psp124BI GAGCTC 2 cut(s) 438, 723
Psp5II RGGWCCY 2 cut(s) 61, 756
Psp6I CCWGG 2 cut(s) 590, 897
PspEI GGTNACC 1 cut(s) 485
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 2 cut(s) 590, 897
PspN4I GGNNCC 7 cut(s) 19, 260, 280, 757, 758, 841, 1055
PspPI GGNCC 5 cut(s) 61, 594, 756, 776, 895
PspPPI RGGWCCY 2 cut(s) 61, 756
PstNI CAGNNNCTG 2 cut(s) 34, 64
PsuI RGATCY 3 cut(s) 240, 463, 713
PvuI CGATCG 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 31
RsaI GTAC 4 cut(s) 458, 833, 962, 1199
RsaNI GTAC 4 cut(s) 457, 832, 961, 1198
SacI GAGCTC 2 cut(s) 438, 723
SacII CCGCGG 1 cut(s) 288
SapI GCTCTTC 2 cut(s) 488, 1139
SaqAI TTAA 2 cut(s) 917, 1137
Sau96I GGNCC 5 cut(s) 61, 594, 756, 776, 895
SchI GAGTC 1 cut(s) 1187
ScrFI CCNGG 2 cut(s) 592, 899
SduI GDGCHC 5 cut(s) 390, 438, 723, 844, 1161
SfaNI GCATC 3 cut(s) 75, 312, 853
SfcI CTRYAG 1 cut(s) 427
Sfr303I CCGCGG 1 cut(s) 288
SgrAI CRCCGGYG 2 cut(s) 70, 955
SgrBI CCGCGG 1 cut(s) 288
SinI GGWCC 4 cut(s) 61, 756, 776, 895
SmlI CTYRAG 1 cut(s) 678
SmoI CTYRAG 1 cut(s) 678
SphI GCATGC 1 cut(s) 421
Sse9I AATT 3 cut(s) 301, 508, 527
SstI GAGCTC 2 cut(s) 438, 723
StyD4I CCNGG 2 cut(s) 590, 897
StyI CCWWGG 2 cut(s) 513, 810
TaaI ACNGT 2 cut(s) 936, 1206
TaiI ACGT 6 cut(s) 95, 170, 224, 270, 750, 774
TasI AATT 3 cut(s) 301, 508, 527
TfiI GAWTC 4 cut(s) 373, 572, 579, 816
Tru1I TTAA 2 cut(s) 917, 1137
Tru9I TTAA 2 cut(s) 917, 1137
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 6 cut(s) 31, 498, 949, 1059, 1062, 1122
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 1 cut(s) 319
TspGWI ACGGA 3 cut(s) 726, 778, 959
Van91I CCANNNNNTGG 1 cut(s) 34
VneI GTGCAC 1 cut(s) 1157
VpaK11BI GGWCC 4 cut(s) 61, 756, 776, 895
XapI RAATTY 1 cut(s) 527
XceI RCATGY 1 cut(s) 421
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.