RLG00000025832

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011068
Physical Location & Seq
Reverse (-)
48574989 .. 48576263
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025832

Sequence Viewer

Length: 1206 bp
ATGCCGAGGCTACCGCTTACGTCCGCGTTCCAGCTCCTCGAGATCAACCTGATCTCGGCACAGGACATCGCGGCTGCGTCCAAGTCTATGCGGACTTTCGCCGTCGCGTGGGTCAACCCTAAGCGGAAGCTAACCACACGTGTGGACCAGAACGGCCACACCAACCCTACGTGGAACGAGAAGTTCGTCTTCCGCGTCGGCGAGGACTTCCTCAAAGACGACACCTCCAAGATCATGATAGAGATCTACGCCTCCGCGTGGCTCCGGGATGTCCTCATCGGCACCGCCGCGATCGTCATCAGTAATCTTCAAAACAACTCCAAGATGCGGTTCATGGCCATCCAGCTCCGGCGACCGTCCGGAAGGCCTCAGGGGATTCTCAACGTGGGACTGGGCCTTTTGGACAACACCATGCGTAGCATGCCGCTCTATAGCGAGCTTAGTGCCTCCGCCGTCGGATATTGGGATATCATGGACGCAAAAAGCAACAAAAAGTCGTCGAAAAACAATGACTCAAAAATGGACGATAAACTTCACTCCTTGTACAGATCGCAGAGTGAACGGACCACAAGTGATTACGGCTTTTCGAAGAGCAAGCCGGGGAGCTCTATATGCAATGCACGTAACAATTACACGAAGAAGCCTCCGGGTTCCATCTGCATTGGAAGTTCTATTGTGAATGGCTCGGAGGTTAGTTCTGCCACGAAAGATGAGACCCCTAGGAAAGGAGGGACGCCTAAGAAAGGTGAGACGCCTAAGAAAGGTGGGACTCCTAAAAATGGGACAAAGACCATTAACAACCCTAATGGCAATGGCTCCATGGTAAATGGTGGCTCCATGGTGAATGGGTCCTTATGCTCGGACGTGGGGCCGTCACCTTCTGTTGTGGCGGCCGCCATTGCAAAGGGAATTTACCCTTTGCCGCATGGGGCATCGCATACAGAGTCAGGGAGCTCGTTGATGGACGAGTGGGCGGACGAAGACAGTGTGGAAGGGTTGAAGACCAAGATTGAGAGGTGGCGCACCGAGTTGCCACCAGTGTATGACACCAAGAATTCGAATTTGAAGCTGATGCCATCGACGCATCGGAGCATTCACCCGAAGCTGAGGCGGAGGAGCCGGAGTAAGCCTAAGAAGAAGAGTAGTGGCAAAGTTCATCTTAGCCCATCTGAACTTACTTACGATGATGGCTCATACGTACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

402

Amino Acids

43.85

Weight (kDa)

9.82

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 108 5.7e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13350 AT3G04360
fragaria_vesca FvH4_6g01610
malus_domestica MD00G1149000.v1.1 MD04G1232000.v1.1
prunus_persica Prupe.6G351400_v2.0.a1
pyrus_communis pycom04g20540 pycom12g22960
rosa_chinensis RchiOBHm_Chr3g0449421
rosa_laevigata RLG00000025832
rosa_multiflora Rmu_sc0019012.1_g000008 Rmu_sc0019478.1_g000001 Rmu_sc0020017.1_g000003
rosa_roxburghii Rroxscaffold_6G00427380
rosa_rugosa Rorug02G0620500
rosa_samantha Rh3AG018300 Rh3BG018600 Rh3CG017500 Rh3DG019000
rosa_wichuraiana Rw3G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 427
AccII CGCG 6 cut(s) 26, 71, 107, 195, 257, 290
AccIII TCCGGA 1 cut(s) 359
AcoI YGGCCR 3 cut(s) 154, 336, 891
AcsI RAATTY 3 cut(s) 909, 1054, 1060
AcvI CACGTG 1 cut(s) 140
AcyI GRCGYC 2 cut(s) 734, 752
AfaI GTAC 2 cut(s) 545, 1200
AfiI CCNNNNNNNGG 8 cut(s) 55, 108, 258, 327, 725, 743, 761, 779
AflIII ACRYGT 2 cut(s) 137, 139
AgsI TTSAA 3 cut(s) 311, 1000, 1066
AjiI CACGTC 1 cut(s) 865
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AleI CACNNNNGTG 1 cut(s) 140
AluBI AGCT 8 cut(s) 34, 130, 346, 439, 606, 954, 1069, 1105
AluI AGCT 8 cut(s) 34, 130, 346, 439, 606, 954, 1069, 1105
Alw21I GWGCWC 2 cut(s) 608, 956
Alw26I GTCTC 2 cut(s) 707, 743
Ama87I CYCGRG 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 6 cut(s) 154, 336, 365, 394, 869, 891
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 3 cut(s) 909, 1054, 1060
AspA2I CCTAGG 1 cut(s) 719
AspLEI GCGC 1 cut(s) 1023
AspS9I GGNCC 5 cut(s) 145, 394, 564, 849, 869
AsuC2I CCSGG 3 cut(s) 266, 600, 648
AsuHPI GGTGA 4 cut(s) 758, 853, 867, 1088
AsuII TTCGAA 2 cut(s) 587, 1058
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 3 cut(s) 145, 564, 849
AvrII CCTAGG 1 cut(s) 719
AxyI CCTNAGG 1 cut(s) 369
BalI TGGCCA 1 cut(s) 338
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 2 cut(s) 608, 956
BbrPI CACGTG 1 cut(s) 140
BbsI GAAGAC 3 cut(s) 181, 987, 1007
Bbv12I GWGCWC 2 cut(s) 608, 956
BbvCI CCTCAGC 1 cut(s) 1106
BbvI GCAGC 1 cut(s) 61
BccI CCATC 6 cut(s) 347, 662, 955, 1084, 1174, 1181
BceAI ACGGC 5 cut(s) 86, 169, 437, 595, 856
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BcnI CCSGG 3 cut(s) 266, 600, 648
BcoDI GTCTC 2 cut(s) 707, 743
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 1 cut(s) 430
BglII AGATCT 1 cut(s) 243
BisI GCNGC 7 cut(s) 72, 75, 288, 425, 891, 894, 923
BlnI CCTAGG 1 cut(s) 719
BlsI GCNGC 7 cut(s) 73, 76, 289, 426, 892, 895, 924
Bme1390I CCNGG 3 cut(s) 266, 600, 648
Bme18I GGWCC 3 cut(s) 145, 564, 849
BmeT110I CYCGRG 1 cut(s) 38
BmgBI CACGTC 1 cut(s) 865
BmgT120I GGNCC 5 cut(s) 145, 394, 564, 849, 869
BmiI GGNNCC 8 cut(s) 263, 283, 652, 817, 835, 850, 870, 1118
BmrFI CCNGG 3 cut(s) 266, 600, 648
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 4 cut(s) 315, 941, 1062, 1093
BmuI ACTGGG 1 cut(s) 401
BpiI GAAGAC 3 cut(s) 181, 987, 1007
Bpu10I CCTNAGC 2 cut(s) 120, 1106
Bpu14I TTCGAA 2 cut(s) 587, 1058
BpuMI CCSGG 3 cut(s) 266, 600, 648
BsaAI YACGTR 4 cut(s) 140, 171, 623, 1198
BsaBI GATNNNNATC 2 cut(s) 242, 296
BsaHI GRCGYC 2 cut(s) 734, 752
BsaI GGTCTC 1 cut(s) 707
BsaJI CCNNGG 5 cut(s) 5, 599, 719, 819, 837
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 4 cut(s) 209, 239, 1108, 1138
Bsc4I CCNNNNNNNGG 8 cut(s) 55, 108, 258, 327, 725, 743, 761, 779
Bse1I ACTGG 2 cut(s) 396, 1037
Bse21I CCTNAGG 1 cut(s) 369
Bse3DI GCAATG 3 cut(s) 622, 817, 897
Bse8I GATNNNNATC 2 cut(s) 242, 296
BseAI TCCGGA 1 cut(s) 359
BseDI CCNNGG 5 cut(s) 5, 599, 719, 819, 837
BseGI GGATG 2 cut(s) 274, 339
BseJI GATNNNNATC 2 cut(s) 242, 296
BseLI CCNNNNNNNGG 8 cut(s) 55, 108, 258, 327, 725, 743, 761, 779
BseMI GCAATG 3 cut(s) 622, 817, 897
BseMII CTCAG 2 cut(s) 383, 1097
BseNI ACTGG 2 cut(s) 396, 1037
BseRI GAGGAG 2 cut(s) 26, 1129
BseX3I CGGCCG 1 cut(s) 891
BseXI GCAGC 1 cut(s) 61
Bsh1236I CGCG 6 cut(s) 26, 71, 107, 195, 257, 290
Bsh1285I CGRYCG 3 cut(s) 294, 356, 894
BshFI GGCC 6 cut(s) 156, 338, 367, 396, 871, 893
BshNI GGYRCC 1 cut(s) 281
BsiEI CGRYCG 3 cut(s) 294, 356, 894
BsiHKAI GWGCWC 2 cut(s) 608, 956
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 6 cut(s) 265, 349, 360, 599, 647, 1120
BsiWI CGTACG 1 cut(s) 1198
BslFI GGGAC 4 cut(s) 402, 745, 781, 796
BslI CCNNNNNNNGG 8 cut(s) 55, 108, 258, 327, 725, 743, 761, 779
BsmAI GTCTC 2 cut(s) 707, 743
BsmBI CGTCTC 1 cut(s) 743
BsmFI GGGAC 4 cut(s) 402, 745, 781, 796
BsmI GAATGC 1 cut(s) 1092
BsnI GGCC 6 cut(s) 156, 338, 367, 396, 871, 893
Bso31I GGTCTC 1 cut(s) 707
BsoBI CYCGRG 1 cut(s) 38
Bsp119I TTCGAA 2 cut(s) 587, 1058
Bsp1286I GDGCHC 2 cut(s) 608, 956
Bsp13I TCCGGA 1 cut(s) 359
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 6 cut(s) 42, 51, 231, 243, 291, 548
Bsp19I CCATGG 2 cut(s) 819, 837
BspANI GGCC 6 cut(s) 156, 338, 367, 396, 871, 893
BspCNI CTCAG 2 cut(s) 382, 1098
BspEI TCCGGA 1 cut(s) 359
BspFNI CGCG 6 cut(s) 26, 71, 107, 195, 257, 290
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 8 cut(s) 263, 283, 652, 817, 835, 850, 870, 1118
BspQI GCTCTTC 1 cut(s) 584
BspT104I TTCGAA 2 cut(s) 587, 1058
BspT107I GGYRCC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 707
BsrBI CCGCTC 1 cut(s) 427
BsrDI GCAATG 3 cut(s) 622, 817, 897
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 2 cut(s) 396, 1037
BssECI CCNNGG 5 cut(s) 5, 599, 719, 819, 837
BssMI GATC 6 cut(s) 42, 51, 231, 243, 291, 548
BssNI GRCGYC 2 cut(s) 734, 752
BssT1I CCWWGG 3 cut(s) 719, 819, 837
Bst4CI ACNGT 2 cut(s) 357, 986
Bst6I CTCTTC 2 cut(s) 584, 1133
BstACI GRCGYC 2 cut(s) 734, 752
BstAUI TGTACA 1 cut(s) 543
BstBAI YACGTR 4 cut(s) 140, 171, 623, 1198
BstBI TTCGAA 2 cut(s) 587, 1058
BstC8I GCNNGC 3 cut(s) 422, 437, 596
BstDEI CTNAG 8 cut(s) 120, 369, 440, 738, 756, 1106, 1131, 1160
BstDSI CCRYGG 2 cut(s) 819, 837
BstENI CCTNNNNNAGG 3 cut(s) 723, 741, 759
BstF5I GGATG 2 cut(s) 274, 339
BstFNI CGCG 6 cut(s) 26, 71, 107, 195, 257, 290
BstHHI GCGC 1 cut(s) 1023
BstKTI GATC 6 cut(s) 45, 54, 234, 246, 294, 551
BstMAI GTCTC 2 cut(s) 707, 743
BstMBI GATC 6 cut(s) 42, 51, 231, 243, 291, 548
BstMCI CGRYCG 3 cut(s) 294, 356, 894
BstMWI GCNNNNNNNGC 4 cut(s) 421, 612, 899, 1081
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 264, 598, 646
BstSFI CTRYAG 1 cut(s) 430
BstSNI TACGTA 1 cut(s) 1198
BstUI CGCG 6 cut(s) 26, 71, 107, 195, 257, 290
BstV1I GCAGC 1 cut(s) 61
BstV2I GAAGAC 3 cut(s) 181, 987, 1007
BstX2I RGATCY 1 cut(s) 243
BstXI CCANNNNNNTGG 1 cut(s) 142
BstYI RGATCY 1 cut(s) 243
BstZI CGGCCG 1 cut(s) 891
Bsu36I CCTNAGG 1 cut(s) 369
BsuRI GGCC 6 cut(s) 156, 338, 367, 396, 871, 893
BtgI CCRYGG 2 cut(s) 819, 837
BtgZI GCGATG 2 cut(s) 52, 918
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 2 cut(s) 274, 339
BtsIMutI CAGTG 2 cut(s) 991, 1044
Cac8I GCNNGC 3 cut(s) 422, 437, 596
CciI TCATGA 1 cut(s) 234
CciNI GCGGCCGC 1 cut(s) 891
CfoI GCGC 1 cut(s) 1023
Cfr13I GGNCC 5 cut(s) 145, 394, 564, 849, 869
CseI GACGC 6 cut(s) 66, 184, 485, 742, 760, 1090
Csp6I GTAC 2 cut(s) 544, 1199
CviAII CATG 8 cut(s) 235, 334, 412, 421, 472, 820, 838, 926
CviQI GTAC 2 cut(s) 544, 1199
DdeI CTNAG 8 cut(s) 120, 369, 440, 738, 756, 1106, 1131, 1160
DpnI GATC 6 cut(s) 44, 53, 233, 245, 293, 550
DpnII GATC 6 cut(s) 42, 51, 231, 243, 291, 548
EaeI YGGCCR 3 cut(s) 154, 336, 891
EagI CGGCCG 1 cut(s) 891
Eam1104I CTCTTC 2 cut(s) 584, 1133
EarI CTCTTC 2 cut(s) 584, 1133
EciI GGCGGA 3 cut(s) 439, 989, 1126
Ecl136II GAGCTC 2 cut(s) 606, 954
EclXI CGGCCG 1 cut(s) 891
Eco105I TACGTA 1 cut(s) 1198
Eco130I CCWWGG 3 cut(s) 719, 819, 837
Eco147I AGGCCT 1 cut(s) 367
Eco24I GRGCYC 2 cut(s) 608, 956
Eco31I GGTCTC 1 cut(s) 707
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 3 cut(s) 145, 564, 849
Eco52I CGGCCG 1 cut(s) 891
Eco53kI GAGCTC 2 cut(s) 606, 954
Eco72I CACGTG 1 cut(s) 140
Eco81I CCTNAGG 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 2 cut(s) 606, 954
EcoNI CCTNNNNNAGG 3 cut(s) 723, 741, 759
EcoO109I RGGNCCY 1 cut(s) 849
EcoRI GAATTC 1 cut(s) 1054
EcoRV GATATC 1 cut(s) 469
EcoT14I CCWWGG 3 cut(s) 719, 819, 837
EcoT38I GRGCYC 2 cut(s) 608, 956
ErhI CCWWGG 3 cut(s) 719, 819, 837
Esp3I CGTCTC 1 cut(s) 743
FaeI CATG 8 cut(s) 238, 337, 415, 424, 475, 823, 841, 929
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 1143, 1175
FaqI GGGAC 4 cut(s) 402, 745, 781, 796
FatI CATG 8 cut(s) 234, 333, 411, 420, 471, 819, 837, 925
Fnu4HI GCNGC 7 cut(s) 72, 75, 288, 425, 891, 894, 923
FokI GGATG 2 cut(s) 281, 326
FriOI GRGCYC 2 cut(s) 608, 956
Fsp4HI GCNGC 7 cut(s) 72, 75, 288, 425, 891, 894, 923
FspBI CTAG 1 cut(s) 720
GlaI GCGC 1 cut(s) 1022
GluI GCNGC 7 cut(s) 72, 75, 288, 425, 891, 894, 923
HaeIII GGCC 6 cut(s) 156, 338, 367, 396, 871, 893
HapII CCGG 6 cut(s) 265, 349, 360, 599, 647, 1120
HgaI GACGC 6 cut(s) 66, 184, 485, 742, 760, 1090
HhaI GCGC 1 cut(s) 1023
Hin1I GRCGYC 2 cut(s) 734, 752
Hin1II CATG 8 cut(s) 238, 337, 415, 424, 475, 823, 841, 929
Hin6I GCGC 1 cut(s) 1021
HinP1I GCGC 1 cut(s) 1021
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 4 cut(s) 376, 512, 769, 944
HpaII CCGG 6 cut(s) 265, 349, 360, 599, 647, 1120
HphI GGTGA 4 cut(s) 758, 853, 867, 1088
Hpy166II GTNNAC 3 cut(s) 115, 145, 560
Hpy188I TCNGA 5 cut(s) 458, 688, 862, 1089, 1171
Hpy188III TCNNGA 3 cut(s) 40, 235, 360
Hpy8I GTNNAC 3 cut(s) 115, 145, 560
Hpy99I CGWCG 5 cut(s) 107, 200, 458, 502, 1084
HpyAV CCTTC 3 cut(s) 357, 888, 986
HpyCH4III ACNGT 2 cut(s) 357, 986
HpyCH4IV ACGT 7 cut(s) 20, 139, 170, 384, 622, 864, 1197
HpyCH4V TGCA 4 cut(s) 615, 620, 660, 902
HpyF10VI GCNNNNNNNGC 4 cut(s) 421, 612, 899, 1081
HpyF3I CTNAG 8 cut(s) 120, 369, 440, 738, 756, 1106, 1131, 1160
HpySE526I ACGT 7 cut(s) 20, 139, 170, 384, 622, 864, 1197
Hsp92I GRCGYC 2 cut(s) 734, 752
Hsp92II CATG 8 cut(s) 238, 337, 415, 424, 475, 823, 841, 929
HspAI GCGC 1 cut(s) 1021
Kpn2I TCCGGA 1 cut(s) 359
Kzo9I GATC 6 cut(s) 42, 51, 231, 243, 291, 548
LguI GCTCTTC 1 cut(s) 584
LmnI GCTCC 9 cut(s) 39, 267, 351, 603, 821, 839, 951, 1089, 1116
Lsp1109I GCAGC 1 cut(s) 61
LweI GCATC 4 cut(s) 315, 941, 1062, 1093
MaeI CTAG 1 cut(s) 720
MaeII ACGT 7 cut(s) 20, 139, 170, 384, 622, 864, 1197
MaeIII GTNAC 2 cut(s) 623, 873
MalI GATC 6 cut(s) 44, 53, 233, 245, 293, 550
MbiI CCGCTC 1 cut(s) 427
MboI GATC 6 cut(s) 42, 51, 231, 243, 291, 548
MboII GAAGA 8 cut(s) 181, 299, 601, 649, 992, 1012, 1147, 1150
MflI RGATCY 1 cut(s) 243
MhlI GDGCHC 2 cut(s) 608, 956
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 4 cut(s) 628, 909, 1054, 1060
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 3 cut(s) 506, 763, 953
MmeI TCCRAC 1 cut(s) 436
Mox20I TGGCCA 1 cut(s) 338
MroI TCCGGA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 338
MseI TTAA 1 cut(s) 795
MslI CAYNNNNRTG 1 cut(s) 140
Msp20I TGGCCA 1 cut(s) 338
MspI CCGG 6 cut(s) 265, 349, 360, 599, 647, 1120
MspR9I CCNGG 3 cut(s) 266, 600, 648
Mva1269I GAATGC 1 cut(s) 1092
MvnI CGCG 6 cut(s) 26, 71, 107, 195, 257, 290
MwoI GCNNNNNNNGC 4 cut(s) 421, 612, 899, 1081
NciI CCSGG 3 cut(s) 266, 600, 648
NcoI CCATGG 2 cut(s) 819, 837
NdeII GATC 6 cut(s) 42, 51, 231, 243, 291, 548
NlaIII CATG 8 cut(s) 238, 337, 415, 424, 475, 823, 841, 929
NlaIV GGNNCC 8 cut(s) 263, 283, 652, 817, 835, 850, 870, 1118
NmeAIII GCCGAG 2 cut(s) 30, 35
NmuCI GTSAC 1 cut(s) 873
NotI GCGGCCGC 1 cut(s) 891
NspI RCATGY 1 cut(s) 424
NspV TTCGAA 2 cut(s) 587, 1058
OliI CACNNNNGTG 1 cut(s) 140
PaeI GCATGC 1 cut(s) 424
PaeR7I CTCGAG 1 cut(s) 38
PagI TCATGA 1 cut(s) 234
PceI AGGCCT 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 584
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 192
PctI GAATGC 1 cut(s) 1092
PfeI GAWTC 1 cut(s) 376
Pfl23II CGTACG 1 cut(s) 1198
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 7 cut(s) 73, 76, 289, 426, 892, 895, 924
Ple19I CGATCG 1 cut(s) 294
PleI GAGTC 3 cut(s) 506, 763, 952
PmaCI CACGTG 1 cut(s) 140
PmlI CACGTG 1 cut(s) 140
PpsI GAGTC 3 cut(s) 506, 763, 952
Ppu21I YACGTR 4 cut(s) 140, 171, 623, 1198
PpuMI RGGWCCY 1 cut(s) 849
Psp124BI GAGCTC 2 cut(s) 608, 956
Psp5II RGGWCCY 1 cut(s) 849
PspCI CACGTG 1 cut(s) 140
PspLI CGTACG 1 cut(s) 1198
PspN4I GGNNCC 8 cut(s) 263, 283, 652, 817, 835, 850, 870, 1118
PspPI GGNCC 5 cut(s) 145, 394, 564, 849, 869
PspPPI RGGWCCY 1 cut(s) 849
PsuI RGATCY 1 cut(s) 243
PvuI CGATCG 1 cut(s) 294
RsaI GTAC 2 cut(s) 545, 1200
RsaNI GTAC 2 cut(s) 544, 1199
RseI CAYNNNNRTG 1 cut(s) 140
SacI GAGCTC 2 cut(s) 608, 956
SapI GCTCTTC 1 cut(s) 584
SaqAI TTAA 1 cut(s) 795
SatI GCNGC 7 cut(s) 72, 75, 288, 425, 891, 894, 923
Sau3AI GATC 6 cut(s) 42, 51, 231, 243, 291, 548
Sau96I GGNCC 5 cut(s) 145, 394, 564, 849, 869
SchI GAGTC 3 cut(s) 506, 763, 953
ScrFI CCNGG 3 cut(s) 266, 600, 648
SduI GDGCHC 2 cut(s) 608, 956
SfaNI GCATC 4 cut(s) 315, 941, 1062, 1093
SfcI CTRYAG 1 cut(s) 430
Sfr274I CTCGAG 1 cut(s) 38
SfuI TTCGAA 2 cut(s) 587, 1058
SinI GGWCC 3 cut(s) 145, 564, 849
SlaI CTCGAG 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
SnaBI TACGTA 1 cut(s) 1198
SphI GCATGC 1 cut(s) 424
Sse9I AATT 4 cut(s) 628, 909, 1054, 1060
SseBI AGGCCT 1 cut(s) 367
SspMI CTAG 1 cut(s) 720
SstI GAGCTC 2 cut(s) 608, 956
StuI AGGCCT 1 cut(s) 367
StyD4I CCNGG 3 cut(s) 264, 598, 646
StyI CCWWGG 3 cut(s) 719, 819, 837
TaaI ACNGT 2 cut(s) 357, 986
TaiI ACGT 7 cut(s) 23, 142, 173, 387, 625, 867, 1200
TaqI TCGA 5 cut(s) 39, 500, 587, 1058, 1079
TasI AATT 4 cut(s) 628, 909, 1054, 1060
TatI WGTACW 1 cut(s) 543
TauI GCSGC 6 cut(s) 74, 290, 427, 893, 896, 925
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 795
Tru9I TTAA 1 cut(s) 795
TscAI CASTG 2 cut(s) 991, 1044
TseFI GTSAC 1 cut(s) 873
TseI GCWGC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 873
TspDTI ATGAA 2 cut(s) 322, 1145
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 2 cut(s) 991, 1044
VpaK11BI GGWCC 3 cut(s) 145, 564, 849
XagI CCTNNNNNAGG 3 cut(s) 723, 741, 759
XapI RAATTY 3 cut(s) 909, 1054, 1060
XceI RCATGY 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 38
XmaJI CCTAGG 1 cut(s) 719
XspI CTAG 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.