MD04G1232000.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011068
Physical Location & Seq
Reverse (-)
31132973 .. 31134181
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1232000.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGCCACGGCCGTTCGGCTCCTCCTTCCAGCTGCTGGAGATCAACCTGATCTCGGCGCAGGACCTGACGCCGGCGTCGAAGGCGATGCGGACGTTCGCCGTCGCGTGGGTCAACCCCAAGCGGAAGCTGACGACGCGTTTCGACCAGACCGGCCACACCAACCCGACGTGGAACGAGAAGTTCGTCTTCCGAGTTGACGACGACTTCCTCCGCCACGACACGTCGAAGATCATGATCGAGATCTACGCCTCCGCATGGCTCCGAGACGTCCTGATCGGAACCGCCGCCGTGGTGGTGAACAATTTGCAGAACAAGTCCAAGATGCGGTTCATGGCGATCCAGCTCCGCCGCCCATCCGGCCGCCCGCAGGGGATTCTGAACATCGGGCTCGGCCTCCTCGACAGCACAATGCGGAGCATGCCGCTCTACAGCGAGCTCAGCTCCTCCGCCGTCGGGTACTGGGATCTGATGGAGGGAAAAACCGGTAACCAGAAGAGCAGCGGCCTCAACTCCAAGGATCAAGACAAATTCGTCATCTTCCAACGATCTCAGAGCGATCGGACCGGCAGCGATTCTGGATTCTCGAAATCCAGGCCCGCATCCTCCATCTGCGATGGCGTCACAAAAATCAGGCCGTCGAACTCCACCTGCAATGCCGCCGCCTACAAGCCCAAAACTCAAGCCTCCTTTTGCAATAGCTCGATCGTGAACGGATCTGAGCTCAGCACAGGGCAAAAGAAAGGTAACGTGAACGGGTCCCTCTGCTCTGACGTCGGTCCTTCGCCCTCTGTGGTTGCGGCCGCAATCGCCAAGGGAATCTACCCGTTGCCGTACACAGGAGCCCGCGCGCCGTCGCCCGATGATGCCGGGAGCTCGTTGCTGGACGAGTGGGCGGACCAGGACAGCGTGGAAGGGTTAAAGACCAAGATAGAACGGTGGCGGACGGAGCTGCCGCCGGTGTACGACTGCGCCAAGAACAACGCAAAGAGTTCCCATATTCAGAAGCCACTCCAATCGTCCCACCGCCGTCCCAGCAGTCAGAAGACGCGGCGGAGCCGCAGCAGTGGCAAAGGATTGTTCTCCTGCTTCGCGTTGGGTTGCGAGATTTCGATCTCTTGCGGGAGCAAACCGAAGATTAACAAGAAGAGCGGGAAAGTGCACCTCAGCCCGTCCGATTTAACTTTCGACGACGACTCGTACATACGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

403

Amino Acids

43.97

Weight (kDa)

9.63

Isoelectric Point (pI)

55.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 11 - 107 2e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13350 AT3G04360
fragaria_vesca FvH4_6g01610
malus_domestica MD00G1149000.v1.1 MD04G1232000.v1.1
prunus_persica Prupe.6G351400_v2.0.a1
pyrus_communis pycom04g20540 pycom12g22960
rosa_chinensis RchiOBHm_Chr3g0449421
rosa_laevigata RLG00000025832
rosa_multiflora Rmu_sc0019012.1_g000008 Rmu_sc0019478.1_g000001 Rmu_sc0020017.1_g000003
rosa_roxburghii Rroxscaffold_6G00427380
rosa_rugosa Rorug02G0620500
rosa_samantha Rh3AG018300 Rh3BG018600 Rh3CG017500 Rh3DG019000
rosa_wichuraiana Rw3G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 656
AasI GACNNNNNNGTC 2 cut(s) 73, 530
AatII GACGTC 2 cut(s) 270, 774
Acc36I ACCTGC 1 cut(s) 656
AccB7I CCANNNNNTGG 1 cut(s) 34
AccBSI CCGCTC 2 cut(s) 424, 1149
AccII CGCG 6 cut(s) 104, 136, 846, 848, 1048, 1091
AclWI GGATC 4 cut(s) 331, 471, 525, 721
AcoI YGGCCR 4 cut(s) 8, 151, 358, 798
AcsI RAATTY 1 cut(s) 527
AcyI GRCGYC 5 cut(s) 68, 74, 267, 618, 771
AfaI GTAC 4 cut(s) 458, 833, 962, 1199
AfiI CCNNNNNNNGG 9 cut(s) 34, 52, 70, 105, 255, 324, 367, 453, 836
AflIII ACRYGT 2 cut(s) 134, 219
AgeI ACCGGT 1 cut(s) 482
AjiI CACGTC 2 cut(s) 168, 222
AjnI CCWGG 2 cut(s) 590, 897
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 9 cut(s) 31, 127, 343, 436, 441, 699, 721, 873, 949
AluI AGCT 9 cut(s) 31, 127, 343, 436, 441, 699, 721, 873, 949
Alw21I GWGCWC 4 cut(s) 438, 723, 875, 1161
Alw26I GTCTC 1 cut(s) 258
Alw44I GTGCAC 1 cut(s) 1157
AlwI GGATC 4 cut(s) 331, 471, 525, 721
AlwNI CAGNNNCTG 2 cut(s) 34, 64
AoxI GGCC 8 cut(s) 8, 151, 358, 391, 502, 593, 632, 798
ApaLI GTGCAC 1 cut(s) 1157
ApeKI GCWGC 5 cut(s) 31, 498, 567, 949, 1059
ApoI RAATTY 1 cut(s) 527
AsiGI ACCGGT 1 cut(s) 482
AspLEI GCGC 4 cut(s) 58, 848, 850, 971
AspS9I GGNCC 6 cut(s) 61, 561, 594, 756, 776, 895
AsuC2I CCSGG 1 cut(s) 868
AsuHPI GGTGA 1 cut(s) 307
AvaII GGWCC 5 cut(s) 61, 561, 756, 776, 895
BaeGI GKGCMC 1 cut(s) 1161
BanII GRGCYC 5 cut(s) 390, 438, 723, 844, 875
BbsI GAAGAC 2 cut(s) 178, 1049
Bbv12I GWGCWC 4 cut(s) 438, 723, 875, 1161
BbvCI CCTCAGC 1 cut(s) 1163
BbvI GCAGC 5 cut(s) 18, 510, 579, 936, 1071
BccI CCATC 4 cut(s) 361, 463, 608, 614
BceAI ACGGC 8 cut(s) 23, 83, 272, 434, 619, 814, 835, 1011
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 592, 899
BcnI CCSGG 1 cut(s) 868
BcoDI GTCTC 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 656
BglI GCCNNNNNGGC 1 cut(s) 357
BglII AGATCT 1 cut(s) 240
BlpI GCTNAGC 2 cut(s) 437, 722
Bme1390I CCNGG 3 cut(s) 592, 868, 899
Bme18I GGWCC 5 cut(s) 61, 561, 756, 776, 895
BmgBI CACGTC 2 cut(s) 168, 222
BmgT120I GGNCC 6 cut(s) 61, 561, 594, 756, 776, 895
BmiI GGNNCC 7 cut(s) 19, 260, 280, 757, 758, 841, 1055
BmrFI CCNGG 3 cut(s) 592, 868, 899
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 4 cut(s) 75, 312, 608, 853
BmuI ACTGGG 1 cut(s) 469
BoxI GACNNNNGTC 1 cut(s) 774
BpiI GAAGAC 2 cut(s) 178, 1049
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BpmI CTGGAG 1 cut(s) 56
Bpu10I CCTNAGC 1 cut(s) 1163
Bpu1102I GCTNAGC 2 cut(s) 437, 722
BpuEI CTTGAG 1 cut(s) 663
BpuMI CCSGG 1 cut(s) 868
BsaBI GATNNNNATC 3 cut(s) 233, 239, 1109
BsaHI GRCGYC 5 cut(s) 68, 74, 267, 618, 771
BsaJI CCNNGG 4 cut(s) 5, 288, 513, 810
BsaWI WCCGGW 1 cut(s) 482
Bsc4I CCNNNNNNNGG 9 cut(s) 34, 52, 70, 105, 255, 324, 367, 453, 836
Bse118I RCCGGY 5 cut(s) 70, 149, 482, 563, 955
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 1 cut(s) 658
Bse8I GATNNNNATC 3 cut(s) 233, 239, 1109
BseBI CCWGG 2 cut(s) 592, 899
BseDI CCNNGG 4 cut(s) 5, 288, 513, 810
BseGI GGATG 2 cut(s) 353, 599
BseJI GATNNNNATC 3 cut(s) 233, 239, 1109
BseLI CCNNNNNNNGG 9 cut(s) 34, 52, 70, 105, 255, 324, 367, 453, 836
BseMI GCAATG 1 cut(s) 658
BseMII CTCAG 5 cut(s) 451, 563, 708, 736, 1177
BseNI ACTGG 1 cut(s) 464
BsePI GCGCGC 1 cut(s) 846
BseRI GAGGAG 3 cut(s) 10, 386, 433
BseSI GKGCMC 1 cut(s) 1161
BseX3I CGGCCG 3 cut(s) 8, 358, 798
BseXI GCAGC 5 cut(s) 18, 510, 579, 936, 1071
BseYI CCCAGC 1 cut(s) 1031
Bsh1236I CGCG 6 cut(s) 104, 136, 846, 848, 1048, 1091
Bsh1285I CGRYCG 5 cut(s) 11, 361, 559, 705, 801
BshFI GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
BshTI ACCGGT 1 cut(s) 482
BsiEI CGRYCG 5 cut(s) 11, 361, 559, 705, 801
BsiHKAI GWGCWC 4 cut(s) 438, 723, 875, 1161
BsiSI CCGG 7 cut(s) 71, 150, 357, 483, 564, 867, 956
BslFI GGGAC 3 cut(s) 742, 1003, 1014
BslI CCNNNNNNNGG 9 cut(s) 34, 52, 70, 105, 255, 324, 367, 453, 836
BsmAI GTCTC 1 cut(s) 258
BsmBI CGTCTC 1 cut(s) 258
BsmFI GGGAC 3 cut(s) 742, 1003, 1014
BsnI GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
Bsp1286I GDGCHC 6 cut(s) 390, 438, 723, 844, 875, 1161
Bsp1720I GCTNAGC 2 cut(s) 437, 722
BspANI GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
BspCNI CTCAG 5 cut(s) 450, 562, 709, 735, 1176
BspFNI CGCG 6 cut(s) 104, 136, 846, 848, 1048, 1091
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 7 cut(s) 19, 260, 280, 757, 758, 841, 1055
BspMI ACCTGC 1 cut(s) 656
BspPI GGATC 4 cut(s) 331, 471, 525, 721
BspQI GCTCTTC 2 cut(s) 488, 1139
BsrBI CCGCTC 2 cut(s) 424, 1149
BsrDI GCAATG 1 cut(s) 658
BsrFI RCCGGY 5 cut(s) 70, 149, 482, 563, 955
BsrI ACTGG 1 cut(s) 464
BssAI RCCGGY 5 cut(s) 70, 149, 482, 563, 955
BssECI CCNNGG 4 cut(s) 5, 288, 513, 810
BssHII GCGCGC 1 cut(s) 846
BssNI GRCGYC 5 cut(s) 68, 74, 267, 618, 771
BssT1I CCWWGG 2 cut(s) 513, 810
Bst2UI CCWGG 2 cut(s) 592, 899
Bst4CI ACNGT 2 cut(s) 936, 1206
Bst6I CTCTTC 2 cut(s) 488, 1139
BstACI GRCGYC 5 cut(s) 68, 74, 267, 618, 771
BstC8I GCNNGC 7 cut(s) 72, 365, 419, 434, 597, 844, 848
BstDEI CTNAG 5 cut(s) 437, 549, 717, 722, 1163
BstDSI CCRYGG 2 cut(s) 5, 288
BstEII GGTNACC 1 cut(s) 485
BstF5I GGATG 2 cut(s) 353, 599
BstFNI CGCG 6 cut(s) 104, 136, 846, 848, 1048, 1091
BstHHI GCGC 4 cut(s) 58, 848, 850, 971
BstMAI GTCTC 1 cut(s) 258
BstMCI CGRYCG 5 cut(s) 11, 361, 559, 705, 801
BstMWI GCNNNNNNNGC 9 cut(s) 80, 133, 357, 418, 438, 806, 946, 1032, 1065
BstNI CCWGG 2 cut(s) 592, 899
BstNSI RCATGY 1 cut(s) 421
BstPAI GACNNNNGTC 1 cut(s) 774
BstPI GGTNACC 1 cut(s) 485
BstSCI CCNGG 3 cut(s) 590, 866, 897
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 1161
BstUI CGCG 6 cut(s) 104, 136, 846, 848, 1048, 1091
BstV1I GCAGC 5 cut(s) 18, 510, 579, 936, 1071
BstV2I GAAGAC 2 cut(s) 178, 1049
BstX2I RGATCY 3 cut(s) 240, 463, 713
BstYI RGATCY 3 cut(s) 240, 463, 713
BstZI CGGCCG 3 cut(s) 8, 358, 798
BsuRI GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
BtgI CCRYGG 2 cut(s) 5, 288
BtgZI GCGATG 2 cut(s) 98, 627
BtrI CACGTC 2 cut(s) 168, 222
BtsCI GGATG 2 cut(s) 353, 599
BtsI GCAGTG 1 cut(s) 1069
BtsIMutI CAGTG 1 cut(s) 1069
BveI ACCTGC 1 cut(s) 656
Cac8I GCNNGC 7 cut(s) 72, 365, 419, 434, 597, 844, 848
CaiI CAGNNNCTG 2 cut(s) 34, 64
CciI TCATGA 1 cut(s) 231
CciNI GCGGCCGC 1 cut(s) 798
CfoI GCGC 4 cut(s) 58, 848, 850, 971
Cfr10I RCCGGY 5 cut(s) 70, 149, 482, 563, 955
Cfr13I GGNCC 6 cut(s) 61, 561, 594, 756, 776, 895
CpoI CGGWCCG 1 cut(s) 561
CseI GACGC 5 cut(s) 63, 76, 142, 607, 1054
Csp6I GTAC 4 cut(s) 457, 832, 961, 1198
CspAI ACCGGT 1 cut(s) 482
CspI CGGWCCG 1 cut(s) 561
CviAII CATG 4 cut(s) 232, 255, 331, 418
CviQI GTAC 4 cut(s) 457, 832, 961, 1198
DdeI CTNAG 5 cut(s) 437, 549, 717, 722, 1163
DrdI GACNNNNNNGTC 2 cut(s) 73, 530
DseDI GACNNNNNNGTC 2 cut(s) 73, 530
EaeI YGGCCR 4 cut(s) 8, 151, 358, 798
EagI CGGCCG 3 cut(s) 8, 358, 798
Eam1104I CTCTTC 2 cut(s) 488, 1139
EarI CTCTTC 2 cut(s) 488, 1139
EciI GGCGGA 6 cut(s) 200, 335, 436, 908, 955, 1066
Ecl136II GAGCTC 3 cut(s) 436, 721, 873
EclXI CGGCCG 3 cut(s) 8, 358, 798
Eco130I CCWWGG 2 cut(s) 513, 810
Eco24I GRGCYC 5 cut(s) 390, 438, 723, 844, 875
Eco47I GGWCC 5 cut(s) 61, 561, 756, 776, 895
Eco52I CGGCCG 3 cut(s) 8, 358, 798
Eco53kI GAGCTC 3 cut(s) 436, 721, 873
Eco91I GGTNACC 1 cut(s) 485
EcoICRI GAGCTC 3 cut(s) 436, 721, 873
EcoO109I RGGNCCY 2 cut(s) 61, 756
EcoO65I GGTNACC 1 cut(s) 485
EcoRII CCWGG 2 cut(s) 590, 897
EcoT14I CCWWGG 2 cut(s) 513, 810
EcoT38I GRGCYC 5 cut(s) 390, 438, 723, 844, 875
ErhI CCWWGG 2 cut(s) 513, 810
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 4 cut(s) 235, 258, 334, 421
FaiI YATR 6 cut(s) 233, 256, 332, 419, 996, 1202
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 3 cut(s) 742, 1003, 1014
FatI CATG 4 cut(s) 231, 254, 330, 417
FauI CCCGC 5 cut(s) 372, 604, 851, 1112, 1142
FokI GGATG 2 cut(s) 340, 586
FriOI GRGCYC 5 cut(s) 390, 438, 723, 844, 875
GlaI GCGC 4 cut(s) 57, 847, 849, 970
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 8 cut(s) 10, 153, 360, 393, 504, 595, 634, 800
HapII CCGG 7 cut(s) 71, 150, 357, 483, 564, 867, 956
HgaI GACGC 5 cut(s) 63, 76, 142, 607, 1054
HhaI GCGC 4 cut(s) 58, 848, 850, 971
Hin1I GRCGYC 5 cut(s) 68, 74, 267, 618, 771
Hin1II CATG 4 cut(s) 235, 258, 334, 421
Hin6I GCGC 4 cut(s) 56, 846, 848, 969
HinP1I GCGC 4 cut(s) 56, 846, 848, 969
HincII GTYRAC 2 cut(s) 112, 196
HindII GTYRAC 2 cut(s) 112, 196
HinfI GANTC 5 cut(s) 373, 572, 579, 816, 1193
HpaII CCGG 7 cut(s) 71, 150, 357, 483, 564, 867, 956
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 8 cut(s) 112, 196, 298, 709, 751, 834, 961, 1159
Hpy188III TCNNGA 7 cut(s) 232, 238, 271, 521, 576, 583, 706
Hpy8I GTNNAC 8 cut(s) 112, 196, 298, 709, 751, 834, 961, 1159
HpyAV CCTTC 4 cut(s) 34, 73, 789, 905
HpyCH4III ACNGT 2 cut(s) 936, 1206
HpyCH4IV ACGT 6 cut(s) 92, 167, 221, 267, 747, 771
HpyCH4V TGCA 4 cut(s) 307, 651, 693, 1159
HpyF10VI GCNNNNNNNGC 9 cut(s) 80, 133, 357, 418, 438, 806, 946, 1032, 1065
HpyF3I CTNAG 5 cut(s) 437, 549, 717, 722, 1163
HpySE526I ACGT 6 cut(s) 92, 167, 221, 267, 747, 771
Hsp92I GRCGYC 5 cut(s) 68, 74, 267, 618, 771
Hsp92II CATG 4 cut(s) 235, 258, 334, 421
HspAI GCGC 4 cut(s) 56, 846, 848, 969
KflI GGGWCCC 1 cut(s) 756
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
LguI GCTCTTC 2 cut(s) 488, 1139
Lsp1109I GCAGC 5 cut(s) 18, 510, 579, 936, 1071
LweI GCATC 4 cut(s) 75, 312, 608, 853
MaeII ACGT 6 cut(s) 92, 167, 221, 267, 747, 771
MaeIII GTNAC 3 cut(s) 485, 619, 743
MbiI CCGCTC 2 cut(s) 424, 1149
MboII GAAGA 7 cut(s) 178, 238, 505, 529, 1054, 1144, 1156
MflI RGATCY 3 cut(s) 240, 463, 713
MhlI GDGCHC 6 cut(s) 390, 438, 723, 844, 875, 1161
MluCI AATT 2 cut(s) 301, 527
MluI ACGCGT 1 cut(s) 134
MlyI GAGTC 1 cut(s) 1187
MmeI TCCRAC 1 cut(s) 565
MreI CGCCGGCG 1 cut(s) 70
MroNI GCCGGC 1 cut(s) 70
MseI TTAA 3 cut(s) 917, 1137, 1178
MspA1I CMGCKG 2 cut(s) 31, 501
MspI CCGG 7 cut(s) 71, 150, 357, 483, 564, 867, 956
MspR9I CCNGG 3 cut(s) 592, 868, 899
MvaI CCWGG 2 cut(s) 592, 899
MvnI CGCG 6 cut(s) 104, 136, 846, 848, 1048, 1091
MwoI GCNNNNNNNGC 9 cut(s) 80, 133, 357, 418, 438, 806, 946, 1032, 1065
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 868
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 4 cut(s) 235, 258, 334, 421
NlaIV GGNNCC 7 cut(s) 19, 260, 280, 757, 758, 841, 1055
NmeAIII GCCGAG 2 cut(s) 32, 369
NmuCI GTSAC 1 cut(s) 619
NotI GCGGCCGC 1 cut(s) 798
NspI RCATGY 1 cut(s) 421
PaeI GCATGC 1 cut(s) 421
PagI TCATGA 1 cut(s) 231
PaqCI CACCTGC 1 cut(s) 656
PauI GCGCGC 1 cut(s) 846
PciSI GCTCTTC 2 cut(s) 488, 1139
PcsI WCGNNNNNNNCGW 3 cut(s) 74, 180, 396
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 4 cut(s) 373, 572, 579, 816
PflMI CCANNNNNTGG 1 cut(s) 34
PinAI ACCGGT 1 cut(s) 482
Ple19I CGATCG 2 cut(s) 559, 705
PleI GAGTC 1 cut(s) 1187
PpsI GAGTC 1 cut(s) 1187
PpuMI RGGWCCY 2 cut(s) 61, 756
PshAI GACNNNNGTC 1 cut(s) 774
Psp124BI GAGCTC 3 cut(s) 438, 723, 875
Psp5II RGGWCCY 2 cut(s) 61, 756
Psp6I CCWGG 2 cut(s) 590, 897
PspEI GGTNACC 1 cut(s) 485
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 2 cut(s) 590, 897
PspN4I GGNNCC 7 cut(s) 19, 260, 280, 757, 758, 841, 1055
PspPI GGNCC 6 cut(s) 61, 561, 594, 756, 776, 895
PspPPI RGGWCCY 2 cut(s) 61, 756
PstNI CAGNNNCTG 2 cut(s) 34, 64
PsuI RGATCY 3 cut(s) 240, 463, 713
PteI GCGCGC 1 cut(s) 846
PvuI CGATCG 2 cut(s) 559, 705
PvuII CAGCTG 1 cut(s) 31
RsaI GTAC 4 cut(s) 458, 833, 962, 1199
RsaNI GTAC 4 cut(s) 457, 832, 961, 1198
Rsr2I CGGWCCG 1 cut(s) 561
RsrII CGGWCCG 1 cut(s) 561
SacI GAGCTC 3 cut(s) 438, 723, 875
SapI GCTCTTC 2 cut(s) 488, 1139
SaqAI TTAA 3 cut(s) 917, 1137, 1178
Sau96I GGNCC 6 cut(s) 61, 561, 594, 756, 776, 895
SchI GAGTC 1 cut(s) 1187
ScrFI CCNGG 3 cut(s) 592, 868, 899
SduI GDGCHC 6 cut(s) 390, 438, 723, 844, 875, 1161
SfaNI GCATC 4 cut(s) 75, 312, 608, 853
SfcI CTRYAG 1 cut(s) 427
SgrAI CRCCGGYG 2 cut(s) 70, 955
SinI GGWCC 5 cut(s) 61, 561, 756, 776, 895
SmlI CTYRAG 1 cut(s) 678
SmoI CTYRAG 1 cut(s) 678
SphI GCATGC 1 cut(s) 421
Sse9I AATT 2 cut(s) 301, 527
SstI GAGCTC 3 cut(s) 438, 723, 875
StyD4I CCNGG 3 cut(s) 590, 866, 897
StyI CCWWGG 2 cut(s) 513, 810
TaaI ACNGT 2 cut(s) 936, 1206
TaiI ACGT 6 cut(s) 95, 170, 224, 270, 750, 774
TaqII GACCGA 1 cut(s) 764
TasI AATT 2 cut(s) 301, 527
TfiI GAWTC 4 cut(s) 373, 572, 579, 816
Tru1I TTAA 3 cut(s) 917, 1137, 1178
Tru9I TTAA 3 cut(s) 917, 1137, 1178
TscAI CASTG 1 cut(s) 1069
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 5 cut(s) 31, 498, 567, 949, 1059
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 1 cut(s) 319
TspGWI ACGGA 2 cut(s) 726, 959
TspRI CASTG 1 cut(s) 1069
Van91I CCANNNNNTGG 1 cut(s) 34
VneI GTGCAC 1 cut(s) 1157
VpaK11BI GGWCC 5 cut(s) 61, 561, 756, 776, 895
XapI RAATTY 1 cut(s) 527
XceI RCATGY 1 cut(s) 421
ZraI GACGTC 2 cut(s) 268, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.