Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012253

Physical Location & Seq

Genomic Coordinates Forward (+)

6147906 .. 6152082

Gene Structure 4177 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G17970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGCTGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCACCGGTCTAACCTTTGCCGTTTCCGACGTATTCGATATTACAGGGTATGTGACTGGATTTGGTCATCCAGACTGGGTCAGGACCCATGAAGCTGCTTCGTCAACATCTCCTGTGGTTTCAACTCTCGTTGAAGGTGGAGCCACTTGTGTTGGAAAAACTGTAGTTGATGAGTTTGCGTTTAGTATTAGTGGAGAAAACAAGCATTACGATTCACCTACAAATCCTGCAGCTCCTACTCGTATTCCTGGTGGCGCTTGCAGTGGAGCTGCTGTAGCCGTAGCAACAAATGCTGTGGATTTTGCTCTAGGCATCGACACAGTTGGCGGGGTAAGAGTGCCTGCTGGATACTGTGGCGTTCTTGGATTCAAATCTTCTTATGGGGCTATTTCAAACACCGGAATCATACCAGTATCTTCAAGTCTTGACTCTGTTGGATGGTTTGCCCGCGATCCAAACACCCTACGTCGTGTTGGCCATGTACTCTTGCAACTTCCATTTGCCACTCAACGGAATCCAAGGCAAATCATACTAGCTGATGACTGTTTTCAGCTGTTAAAGATCCCTGTGGATCGGATTACCCAGGTGGTGACCAAATCAGCTGAAAAGCTTTTTGGAAGACAATTGCTAAAGCATCAGAACTTGGAGACCTATTTTGAAACTAAAGTTCCTAGCTTGAAAGAGTTCGCTAGGACAAAAGCCATTGCTAACACGAAGGTCTCAACATCGAGACTACTGGCGAATGTGATGCAGCTCCTTCAAAGGCACGAGTTCCTTCAAAATCATGGGGATTGGATCAACACAGTGAAGCCAGCCATTGATCCTGTGATTTTATCACAAGTGTGTGAGAACCCAGAACTAACCAATGAAGAGACTGAGAACCTGAATGCTATCAGAAACGAGACTCGAGTGGCTATTGGTTCACTTCTCAAGGATGATGGCATTCTGGTAATTCCAACATTGCCAGCTGTTCCTCCAAAACTTGGTAGCAAGGAGATAACATCTGAAGACTATCAAAACCGAGCTTCAAGTCTGCTTAGCATTGCTAGCATATCAGGATGTTGTCAGGTGACTGTGCCACTCGGACATCATGAGAAATGCCCTATTTCAGTTTCTTTCATAGGAAGACACGGTGGTGACCGCTTTTTACTAGATACAGTGCAGACAATGTATCCATCTTTGCAAGAGTACTCGAGTATTGTTACCGATCCCAAATCTTCTAAAAAGGCTATCACCAAAGAAGAGTCAGCCGAGATTGCCAAAGAGAAGGGTAACCAAGCGTTCAAAGAGAAACTATGGCAGAAAGCTATTGGTCTATACTCAGAAGCTATAAAGCTAAGTGACAACAATGCTACCTATTACAGTAACAGAGCTGCTGCATATCTTGAACTTGGAGGCTTTCTTCAAGCCGAAGAAGACTGTACCAAAGCCATCACCCTCGACAAGAAGAATGTTAAAGCCTATTTACGAAGAGGAACCGCAAGAGAAATGTTAGGCGATTGCAAGGGAGCCATAGAAGATTTCAGATACGCACTTGTGCTGGAACCAAACAACAAACGAGCGTCTCTCTCAGCAGAGAGACTGAGAAAATTCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0016020 GO:0016021 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase TPR_11 TPR_16 TPR_2 TPR_8

Protein Analysis

589

Amino Acids

64.02

Weight (kDa)

8.62

Isoelectric Point (pI)

34.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	55 - 441	3.8e-45	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012253)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17970 AT3G17970
fragaria_vesca	FvH4_5g17380
malus_domestica	MD06G1227100.v1.1 MD14G1236500.v1.1
prunus_persica	Prupe.5G233600_v2.0.a1
pyrus_communis	pycom06g20340 pycom14g19750
rosa_chinensis	RchiOBHm_Chr7g0178361
rosa_laevigata	RLG00000005452
rosa_roxburghii	Rroxscaffold_3G00274980
rosa_rugosa	Rorug06G0416300 Rorug06G0416400
rosa_samantha	Rh7AG015100 Rh7BG014600 Rh7CG014600 Rh7DG014900
rosa_wichuraiana	Rw0G010200 Rw7G001200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	154
AccB7I	CCANNNNNTGG	1 cut(s)	1154
AccII	CGCG	1 cut(s)	621
AciI	CCGC	5 cut(s)	140, 498, 619, 1312, 1650
AclWI	GGATC	6 cut(s)	617, 727, 750, 974, 986, 1373
AcoI	YGGCCR	1 cut(s)	646
AcsI	RAATTY	1 cut(s)	1760
AcuI	CTGAAG	2 cut(s)	98, 1197
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	3 cut(s)	654, 1361, 1594
AfiI	CCNNNNNNNGG	3 cut(s)	176, 681, 1154
AgeI	ACCGGT	1 cut(s)	176
AjnI	CCWGG	2 cut(s)	418, 753
AjuI	GAANNNNNNNTTGG	2 cut(s)	768, 800
AleI	CACNNNNGTG	2 cut(s)	1012, 1305
Alw26I	GTCTC	9 cut(s)	12, 50, 812, 895, 895, 1037, 1067, 1740, 1744
AlwI	GGATC	6 cut(s)	617, 727, 750, 974, 986, 1373
Ama87I	CYCGRG	2 cut(s)	1077, 1363
AoxI	GGCC	1 cut(s)	646
ApeKI	GCWGC	7 cut(s)	14, 266, 401, 440, 922, 1544, 1547
ApoI	RAATTY	1 cut(s)	1760
AsiGI	ACCGGT	1 cut(s)	176
Asp700I	GAANNNNTTC	1 cut(s)	854
AspLEI	GCGC	2 cut(s)	107, 428
AspS9I	GGNCC	1 cut(s)	255
AsuHPI	GGTGA	7 cut(s)	166, 378, 772, 1252, 1319, 1396, 1597
AsuNHI	GCTAGC	1 cut(s)	1217
AvaI	CYCGRG	2 cut(s)	1077, 1363
AvaII	GGWCC	1 cut(s)	255
BalI	TGGCCA	1 cut(s)	648
BauI	CACGAG	1 cut(s)	938
BbsI	GAAGAC	5 cut(s)	89, 796, 1185, 1303, 1593
BbvI	GCAGC	6 cut(s)	253, 413, 427, 934, 1531, 1534
BccI	CCATC	4 cut(s)	603, 1103, 1354, 1610
BceAI	ACGGC	2 cut(s)	176, 434
BcgI	CGANNNNNNTGC	2 cut(s)	901, 935
BciT130I	CCWGG	2 cut(s)	420, 755
BciVI	GTATCC	2 cut(s)	512, 1353
BcoDI	GTCTC	9 cut(s)	12, 50, 812, 895, 895, 1037, 1067, 1740, 1744
BfaI	CTAG	7 cut(s)	35, 479, 704, 843, 861, 1218, 1322
BfmI	CTRYAG	3 cut(s)	333, 399, 444
BfoI	RGCGCY	2 cut(s)	108, 429
BfuAI	ACCTGC	1 cut(s)	154
BfuI	GTATCC	2 cut(s)	512, 1353
BisI	GCNGC	7 cut(s)	15, 267, 402, 441, 923, 1545, 1548
BlpI	GCTNAGC	1 cut(s)	1208
BlsI	GCNGC	7 cut(s)	16, 268, 403, 442, 924, 1546, 1549
BmcAI	AGTACT	1 cut(s)	1361
Bme1390I	CCNGG	2 cut(s)	420, 755
Bme18I	GGWCC	1 cut(s)	255
BmeT110I	CYCGRG	2 cut(s)	1077, 1363
BmgT120I	GGNCC	1 cut(s)	255
BmiI	GGNNCC	5 cut(s)	257, 313, 1648, 1681, 1716
BmrFI	CCNGG	2 cut(s)	420, 755
BmrI	ACTGGG	1 cut(s)	256
BmsI	GCATC	3 cut(s)	492, 814, 909
BmtI	GCTAGC	1 cut(s)	1221
BmuI	ACTGGG	1 cut(s)	256
BpiI	GAAGAC	5 cut(s)	89, 796, 1185, 1303, 1593
BplI	GAGNNNNNCTC	2 cut(s)	1722, 1754
Bpu1102I	GCTNAGC	1 cut(s)	1208
BpuEI	CTTGAG	1 cut(s)	1085
Bsa29I	ATCGAT	1 cut(s)	114
BsaBI	GATNNNNATC	1 cut(s)	541
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	3 cut(s)	50, 812, 895
BsaJI	CCNNGG	2 cut(s)	689, 753
BsaWI	WCCGGW	2 cut(s)	176, 569
BsaXI	ACNNNNNCTCC	2 cut(s)	429, 459
Bsc4I	CCNNNNNNNGG	3 cut(s)	176, 681, 1154
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	5 cut(s)	232, 251, 581, 912, 1765
Bse3DI	GCAATG	3 cut(s)	873, 1130, 1212
Bse8I	GATNNNNATC	1 cut(s)	541
BseBI	CCWGG	2 cut(s)	420, 755
BseCI	ATCGAT	1 cut(s)	114
BseDI	CCNNGG	2 cut(s)	689, 753
BseGI	GGATG	5 cut(s)	166, 238, 614, 1111, 1235
BseJI	GATNNNNATC	1 cut(s)	541
BseLI	CCNNNNNNNGG	3 cut(s)	176, 681, 1154
BseMI	GCAATG	3 cut(s)	873, 1130, 1212
BseMII	CTCAG	4 cut(s)	1038, 1506, 1745, 1755
BseNI	ACTGG	5 cut(s)	232, 251, 581, 912, 1765
BseRI	GAGGAG	2 cut(s)	141, 153
BseXI	GCAGC	6 cut(s)	253, 413, 427, 934, 1531, 1534
BseYI	CCCAGC	1 cut(s)	50
BsgI	GTGCAG	1 cut(s)	1352
Bsh1236I	CGCG	1 cut(s)	621
BshFI	GGCC	1 cut(s)	648
BshTI	ACCGGT	1 cut(s)	176
BshVI	ATCGAT	1 cut(s)	114
BsiHKCI	CYCGRG	2 cut(s)	1077, 1363
BsiSI	CCGG	2 cut(s)	177, 570
BslI	CCNNNNNNNGG	3 cut(s)	176, 681, 1154
BsmAI	GTCTC	9 cut(s)	12, 50, 812, 895, 895, 1037, 1067, 1740, 1744
BsmBI	CGTCTC	2 cut(s)	12, 1740
BsmI	GAATGC	2 cut(s)	1063, 1113
BsnI	GGCC	1 cut(s)	648
Bso31I	GGTCTC	3 cut(s)	50, 812, 895
BsoBI	CYCGRG	2 cut(s)	1077, 1363
Bsp143I	GATC	6 cut(s)	622, 732, 742, 966, 991, 1378
Bsp1720I	GCTNAGC	1 cut(s)	1208
BspACI	CCGC	5 cut(s)	140, 498, 619, 1312, 1650
BspANI	GGCC	1 cut(s)	648
BspCNI	CTCAG	4 cut(s)	1039, 1505, 1746, 1754
BspDI	ATCGAT	1 cut(s)	114
BspFNI	CGCG	1 cut(s)	621
BspHI	TCATGA	1 cut(s)	1261
BspLI	GGNNCC	5 cut(s)	257, 313, 1648, 1681, 1716
BspMAI	CTGCAG	1 cut(s)	403
BspMI	ACCTGC	1 cut(s)	154
BspOI	GCTAGC	1 cut(s)	1221
BspPI	GGATC	6 cut(s)	617, 727, 750, 974, 986, 1373
BspTNI	GGTCTC	3 cut(s)	50, 812, 895
BsrDI	GCAATG	3 cut(s)	873, 1130, 1212
BsrFI	RCCGGY	1 cut(s)	176
BsrI	ACTGG	5 cut(s)	232, 251, 581, 912, 1765
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	2 cut(s)	689, 753
BssMI	GATC	6 cut(s)	622, 732, 742, 966, 991, 1378
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	938
BssT1I	CCWWGG	1 cut(s)	689
Bst2BI	CACGAG	1 cut(s)	938
Bst2UI	CCWGG	2 cut(s)	420, 755
Bst6I	CTCTTC	3 cut(s)	1035, 1407, 1636
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	1 cut(s)	461
BstC8I	GCNNGC	6 cut(s)	430, 513, 619, 984, 1137, 1219
BstDEI	CTNAG	6 cut(s)	1047, 1208, 1492, 1508, 1741, 1754
BstEII	GGTNACC	3 cut(s)	760, 1307, 1442
BstF5I	GGATG	5 cut(s)	166, 238, 614, 1111, 1235
BstFNI	CGCG	1 cut(s)	621
BstH2I	RGCGCY	2 cut(s)	108, 429
BstHHI	GCGC	2 cut(s)	107, 428
BstKTI	GATC	6 cut(s)	625, 735, 745, 969, 994, 1381
BstMAI	GTCTC	9 cut(s)	12, 50, 812, 895, 895, 1037, 1067, 1740, 1744
BstMBI	GATC	6 cut(s)	622, 732, 742, 966, 991, 1378
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 446, 461, 1218, 1427
BstNI	CCWGG	2 cut(s)	420, 755
BstPI	GGTNACC	3 cut(s)	760, 1307, 1442
BstSCI	CCNGG	2 cut(s)	418, 753
BstSFI	CTRYAG	3 cut(s)	333, 399, 444
BstUI	CGCG	1 cut(s)	621
BstV1I	GCAGC	6 cut(s)	253, 413, 427, 934, 1531, 1534
BstV2I	GAAGAC	5 cut(s)	89, 796, 1185, 1303, 1593
BstX2I	RGATCY	1 cut(s)	732
BstYI	RGATCY	1 cut(s)	732
Bsu15I	ATCGAT	1 cut(s)	114
BsuI	GTATCC	2 cut(s)	512, 1353
BsuRI	GGCC	1 cut(s)	648
BsuTUI	ATCGAT	1 cut(s)	114
BtsCI	GGATG	5 cut(s)	166, 238, 614, 1111, 1235
BtsI	GCAGTG	1 cut(s)	439
BtsIMutI	CAGTG	3 cut(s)	439, 981, 1335
BveI	ACCTGC	1 cut(s)	154
Cac8I	GCNNGC	6 cut(s)	430, 513, 619, 984, 1137, 1219
CciI	TCATGA	1 cut(s)	1261
CfoI	GCGC	2 cut(s)	107, 428
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	1 cut(s)	255
ClaI	ATCGAT	1 cut(s)	114
CseI	GACGC	1 cut(s)	1722
Csp6I	GTAC	3 cut(s)	653, 1360, 1593
CspAI	ACCGGT	1 cut(s)	176
CspCI	CAANNNNNGTGG	4 cut(s)	304, 339, 447, 482
CviAII	CATG	5 cut(s)	124, 260, 650, 956, 1262
CviQI	GTAC	3 cut(s)	653, 1360, 1593
DdeI	CTNAG	6 cut(s)	1047, 1208, 1492, 1508, 1741, 1754
DpnI	GATC	6 cut(s)	624, 734, 744, 968, 993, 1380
DpnII	GATC	6 cut(s)	622, 732, 742, 966, 991, 1378
EaeI	YGGCCR	1 cut(s)	646
Eam1104I	CTCTTC	3 cut(s)	1035, 1407, 1636
EarI	CTCTTC	3 cut(s)	1035, 1407, 1636
Eco130I	CCWWGG	1 cut(s)	689
Eco31I	GGTCTC	3 cut(s)	50, 812, 895
Eco47I	GGWCC	1 cut(s)	255
Eco57I	CTGAAG	2 cut(s)	98, 1197
Eco88I	CYCGRG	2 cut(s)	1077, 1363
Eco91I	GGTNACC	3 cut(s)	760, 1307, 1442
EcoO109I	RGGNCCY	1 cut(s)	255
EcoO65I	GGTNACC	3 cut(s)	760, 1307, 1442
EcoRII	CCWGG	2 cut(s)	418, 753
EcoT14I	CCWWGG	1 cut(s)	689
ErhI	CCWWGG	1 cut(s)	689
Esp3I	CGTCTC	2 cut(s)	12, 1740
FaeI	CATG	5 cut(s)	127, 263, 653, 959, 1265
FatI	CATG	5 cut(s)	123, 259, 649, 955, 1261
FauI	CCCGC	2 cut(s)	491, 626
Fnu4HI	GCNGC	7 cut(s)	15, 267, 402, 441, 923, 1545, 1548
FokI	GGATG	5 cut(s)	153, 225, 621, 1118, 1242
Fsp4HI	GCNGC	7 cut(s)	15, 267, 402, 441, 923, 1545, 1548
FspBI	CTAG	7 cut(s)	35, 479, 704, 843, 861, 1218, 1322
GlaI	GCGC	2 cut(s)	106, 427
GluI	GCNGC	7 cut(s)	15, 267, 402, 441, 923, 1545, 1548
GsaI	CCCAGC	1 cut(s)	54
HaeII	RGCGCY	2 cut(s)	108, 429
HaeIII	GGCC	1 cut(s)	648
HapII	CCGG	2 cut(s)	177, 570
HgaI	GACGC	1 cut(s)	1722
HhaI	GCGC	2 cut(s)	107, 428
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	5 cut(s)	127, 263, 653, 959, 1265
Hin6I	GCGC	2 cut(s)	105, 426
HinP1I	GCGC	2 cut(s)	105, 426
HincII	GTYRAC	1 cut(s)	276
HindII	GTYRAC	1 cut(s)	276
HindIII	AAGCTT	1 cut(s)	779
HinfI	GANTC	9 cut(s)	19, 55, 383, 537, 573, 599, 685, 1075, 1415
HpaII	CCGG	2 cut(s)	177, 570
HphI	GGTGA	7 cut(s)	166, 378, 772, 1252, 1319, 1396, 1597
Hpy166II	GTNNAC	2 cut(s)	276, 1094
Hpy188I	TCNGA	8 cut(s)	199, 747, 810, 1067, 1177, 1256, 1495, 1697
Hpy188III	TCNNGA	7 cut(s)	242, 253, 596, 900, 1227, 1262, 1556
Hpy8I	GTNNAC	2 cut(s)	276, 1094
Hpy99I	CGWCG	2 cut(s)	203, 642
HpyAV	CCTTC	5 cut(s)	299, 880, 938, 956, 1432
HpyCH4IV	ACGT	2 cut(s)	201, 637
HpyCH4V	TGCA	8 cut(s)	401, 432, 661, 922, 1333, 1354, 1550, 1674
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 446, 461, 1218, 1427
HpyF3I	CTNAG	6 cut(s)	1047, 1208, 1492, 1508, 1741, 1754
HpySE526I	ACGT	2 cut(s)	201, 637
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	5 cut(s)	127, 263, 653, 959, 1265
HspAI	GCGC	2 cut(s)	105, 426
Kzo9I	GATC	6 cut(s)	622, 732, 742, 966, 991, 1378
LmnI	GCTCC	7 cut(s)	154, 166, 311, 409, 437, 930, 1679
Lsp1109I	GCAGC	6 cut(s)	253, 413, 427, 934, 1531, 1534
LweI	GCATC	3 cut(s)	492, 814, 909
MaeI	CTAG	7 cut(s)	35, 479, 704, 843, 861, 1218, 1322
MaeII	ACGT	2 cut(s)	201, 637
MaeIII	GTNAC	8 cut(s)	223, 760, 1240, 1307, 1372, 1442, 1511, 1535
MalI	GATC	6 cut(s)	624, 734, 744, 968, 993, 1380
MboI	GATC	6 cut(s)	622, 732, 742, 966, 991, 1378
MfeI	CAATTG	1 cut(s)	794
MflI	RGATCY	1 cut(s)	732
MlsI	TGGCCA	1 cut(s)	648
MluCI	AATT	3 cut(s)	794, 1122, 1760
MluNI	TGGCCA	1 cut(s)	648
MlyI	GAGTC	3 cut(s)	593, 1069, 1424
MmeI	TCCRAC	4 cut(s)	222, 304, 586, 1151
MnlI	CCTC	8 cut(s)	147, 162, 174, 180, 1155, 1559, 1619, 1637
Mox20I	TGGCCA	1 cut(s)	648
MroXI	GAANNNNTTC	1 cut(s)	854
MscI	TGGCCA	1 cut(s)	648
MseI	TTAA	2 cut(s)	728, 1626
MslI	CAYNNNNRTG	2 cut(s)	1012, 1305
Msp20I	TGGCCA	1 cut(s)	648
MspA1I	CMGCKG	3 cut(s)	724, 773, 1139
MspI	CCGG	2 cut(s)	177, 570
MspR9I	CCNGG	2 cut(s)	420, 755
MunI	CAATTG	1 cut(s)	794
Mva1269I	GAATGC	2 cut(s)	1063, 1113
MvaI	CCWGG	2 cut(s)	420, 755
MvnI	CGCG	1 cut(s)	621
MwoI	GCNNNNNNNGC	5 cut(s)	11, 446, 461, 1218, 1427
NdeII	GATC	6 cut(s)	622, 732, 742, 966, 991, 1378
NheI	GCTAGC	1 cut(s)	1217
NlaIII	CATG	5 cut(s)	127, 263, 653, 959, 1265
NlaIV	GGNNCC	5 cut(s)	257, 313, 1648, 1681, 1716
NmeAIII	GCCGAG	1 cut(s)	1447
NmuCI	GTSAC	5 cut(s)	223, 760, 1240, 1307, 1511
OliI	CACNNNNGTG	2 cut(s)	1012, 1305
PaeR7I	CTCGAG	2 cut(s)	1077, 1363
PagI	TCATGA	1 cut(s)	1261
PctI	GAATGC	2 cut(s)	1063, 1113
PdmI	GAANNNNTTC	1 cut(s)	854
PfeI	GAWTC	6 cut(s)	19, 55, 383, 537, 573, 685
PflFI	GACNNNGTC	1 cut(s)	248
PflMI	CCANNNNNTGG	1 cut(s)	1154
PinAI	ACCGGT	1 cut(s)	176
PkrI	GCNGC	7 cut(s)	16, 268, 403, 442, 924, 1546, 1549
PleI	GAGTC	3 cut(s)	593, 1069, 1423
PpsI	GAGTC	3 cut(s)	593, 1069, 1423
PpuMI	RGGWCCY	1 cut(s)	255
Psp5II	RGGWCCY	1 cut(s)	255
Psp6I	CCWGG	2 cut(s)	418, 753
PspEI	GGTNACC	3 cut(s)	760, 1307, 1442
PspFI	CCCAGC	1 cut(s)	50
PspGI	CCWGG	2 cut(s)	418, 753
PspN4I	GGNNCC	5 cut(s)	257, 313, 1648, 1681, 1716
PspPI	GGNCC	1 cut(s)	255
PspPPI	RGGWCCY	1 cut(s)	255
PspXI	VCTCGAGB	2 cut(s)	1077, 1363
PstI	CTGCAG	1 cut(s)	403
PsuI	RGATCY	1 cut(s)	732
PsyI	GACNNNGTC	1 cut(s)	248
PvuII	CAGCTG	3 cut(s)	724, 773, 1139
RsaI	GTAC	3 cut(s)	654, 1361, 1594
RsaNI	GTAC	3 cut(s)	653, 1360, 1593
RseI	CAYNNNNRTG	2 cut(s)	1012, 1305
SaqAI	TTAA	2 cut(s)	728, 1626
SatI	GCNGC	7 cut(s)	15, 267, 402, 441, 923, 1545, 1548
Sau3AI	GATC	6 cut(s)	622, 732, 742, 966, 991, 1378
Sau96I	GGNCC	1 cut(s)	255
ScaI	AGTACT	1 cut(s)	1361
SchI	GAGTC	3 cut(s)	593, 1069, 1424
ScrFI	CCNGG	2 cut(s)	420, 755
SfaNI	GCATC	3 cut(s)	492, 814, 909
SfcI	CTRYAG	3 cut(s)	333, 399, 444
Sfr274I	CTCGAG	2 cut(s)	1077, 1363
SinI	GGWCC	1 cut(s)	255
SlaI	CTCGAG	2 cut(s)	1077, 1363
SmiMI	CAYNNNNRTG	2 cut(s)	1012, 1305
SmlI	CTYRAG	4 cut(s)	9, 1077, 1100, 1363
SmoI	CTYRAG	4 cut(s)	9, 1077, 1100, 1363
Sse9I	AATT	3 cut(s)	794, 1122, 1760
SsiI	CCGC	5 cut(s)	140, 498, 619, 1312, 1650
SspMI	CTAG	7 cut(s)	35, 479, 704, 843, 861, 1218, 1322
StyD4I	CCNGG	2 cut(s)	418, 753
StyI	CCWWGG	1 cut(s)	689
TaiI	ACGT	2 cut(s)	204, 640
TaqI	TCGA	7 cut(s)	114, 207, 486, 899, 1078, 1364, 1611
TasI	AATT	3 cut(s)	794, 1122, 1760
TatI	WGTACW	2 cut(s)	652, 1359
TfiI	GAWTC	6 cut(s)	19, 55, 383, 537, 573, 685
Tru1I	TTAA	2 cut(s)	728, 1626
Tru9I	TTAA	2 cut(s)	728, 1626
TscAI	CASTG	3 cut(s)	439, 981, 1335
TseFI	GTSAC	5 cut(s)	223, 760, 1240, 1307, 1511
TseI	GCWGC	7 cut(s)	14, 266, 401, 440, 922, 1544, 1547
Tsp45I	GTSAC	5 cut(s)	223, 760, 1240, 1307, 1511
TspDTI	ATGAA	3 cut(s)	276, 1053, 1279
TspGWI	ACGGA	2 cut(s)	80, 697
TspRI	CASTG	3 cut(s)	439, 981, 1335
Tth111I	GACNNNGTC	1 cut(s)	248
Van91I	CCANNNNNTGG	1 cut(s)	1154
VpaK11BI	GGWCC	1 cut(s)	255
XapI	RAATTY	1 cut(s)	1760
XhoI	CTCGAG	2 cut(s)	1077, 1363
XmnI	GAANNNNTTC	1 cut(s)	854
XspI	CTAG	7 cut(s)	35, 479, 704, 843, 861, 1218, 1322
ZrmI	AGTACT	1 cut(s)	1361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.