Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012253

Physical Location & Seq

Genomic Coordinates Reverse (-)

35776140 .. 35781553

Gene Structure 5414 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1227100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1773 bp

ATGGCGTCTCAGGCCGCCAATCTGTGGGTCCTGGTGGGTTTGGGTTTGGCTGGAATTCTCCTGGTGACAAGGAAGCTGACGAAGACCATCAGAGAAGACTTTGGCGCCTTTATTGAGCGGCTTCAGCTTCTTCCTCCGCCGCAGCCTGCTCCTCCCAAGGCTCCGCATCCCCTCACCGGCCTCACCTTCGCCGTCTCCGACGTATTTGAAATTGAGGGACATGTAACTGGTTTTGGTCATCCTGATTGGGGAAGAACACATGATGCAGCTTCTCGGACATCGCCTGTGGTTTCCACTCTTGTCGAAGGAGGTGCCACATGCACTGGGATAACTGTTGTGGATGAATTGGCATATAGTATTACTGGAGAAAATAAGCATTACGGTACCCCCACCAATCCAGCAGCATCTTCCAGAACACCAGGTGGATCCTCTAGTGGAGCTGCCGTGGCTGTAGCCGCTGATCTTGTTGATTTTTCACTCGGCCTTGATACTGTTGGTGGCGTTAGAGTACCTGCTGGGTCTTGTGGCATCATTGGATTCCGAGCTTCGCATGGCTCTGTTTCTCATGCTGGATTTATACCTGTTTCTACAAGTCTGGATACTGTTGGATGGTTTGCTAGGGATCCAAGTGTCCTGCGTCGTGTTGGACACGTGCTGCTGCAACTTCCTTATGCAGTTTCACGCAGTCCAAGACAAATTGTAATAGCTGATGATTGCTTTCAAGAAGTAAAGATTCCCATTGATAGGATTGTTCAAGTGGTTATCAAATCCACTGAGAAGCTTTTTGGAAAACAAGTGTTGAAACATGAAAATCTTAGGGACTATTTCAATTCAAAAATTCCAAGCTTGAAAGAGTTTCATGGCCAGAAGACAAATGGTGAAGTAAAGACTTCATCCCTCAAATTGCTTGCCAATGTTGTGCAGTTTCTTCAAAGGCATGAGTTCAGACATAATCATGGGGAGTGGATTGATTCGGTGAAGCCTGTTTTGGAGTCTGCTACTTCAATGCAAATATGTGGGCCACTGGAGGCATCCGATGAGGAGATTGAGATATGCAAGTCAATTAGGAATGAATTACAGGCAGCTGTCAGCTCTCTATTGAAGGATGATGGAATTTTGGTTATCCCAACTATAGCTGACCCCCCTTCGAAACTTGGTGGGAAGGAGATGCTCTCTGAGGACTTCCGCAGTCGTGCTTTTGGTCTCTTAAGTATTGCGAGCCTGTCAGGTTGTTGTCAGGTCACAGTACCTCTGGGTTTTCATGACAAGTGTCCTGTTTCAGTTTCTTTCATAGCCAAGCATGGGGGTGATCGCTTTTTACTGGATGCACTGCACACTATGTACACATCTTTACAGGAGCAGGCTGATATTGCTTCAAAATCCAAATCATCCAAAAATGCTCTCACCAAGGAACAGTCTGCTGAAATTGCCAAGGAAAAGGGAAATCGAGCATACAAAGATAAGGACTGGCAGAAGGCTATTGGCTTTTATTCTGAAGCTATCAAACTTAGTGGCAACAATGCAACGTATTACAGTAACAGGGCTCAAGCATATCTTGAAGTTGAAAGATTCATTCAAGCCGAGGCAGATTGTACTAAAGCTATCAACCTTGACAAAAAGAATGTGAAAGCTTATTTCCGAAGAGGCACTGCAAGGGAGATGCTTGGTTATTACAAGGAGGCGATTGAAGACTTTAGACATGCACTTGTTCTTGAGCCAACCAACAAACGAGCAGCCGCTGCTGCTGACAGATTGAGGAAGCTGTTCCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0016020 GO:0016021 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase TPR_11 TPR_16 TPR_2 TPR_8

Protein Analysis

591

Amino Acids

64.17

Weight (kDa)

8.55

Isoelectric Point (pI)

35.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	55 - 219	7.5e-39	Amidase
TPR_BSK1_C	PF25575	441 - 540	8.4e-07	Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_2	PF07719	543 - 575	3.5e-06	Tetratricopeptide repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012253)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17970 AT3G17970
fragaria_vesca	FvH4_5g17380
malus_domestica	MD06G1227100.v1.1 MD14G1236500.v1.1
prunus_persica	Prupe.5G233600_v2.0.a1
pyrus_communis	pycom06g20340 pycom14g19750
rosa_chinensis	RchiOBHm_Chr7g0178361
rosa_laevigata	RLG00000005452
rosa_roxburghii	Rroxscaffold_3G00274980
rosa_rugosa	Rorug06G0416300 Rorug06G0416400
rosa_samantha	Rh7AG015100 Rh7BG014600 Rh7CG014600 Rh7DG014900
rosa_wichuraiana	Rw0G010200 Rw7G001200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	520
Acc65I	GGTACC	1 cut(s)	383
AccB1I	GGYRCC	3 cut(s)	104, 311, 383
AccB7I	CCANNNNNTGG	1 cut(s)	24
AccBSI	CCGCTC	1 cut(s)	118
AciI	CCGC	8 cut(s)	15, 118, 137, 140, 164, 456, 1186, 1737
AclWI	GGATC	4 cut(s)	420, 433, 617, 630
AcoI	YGGCCR	1 cut(s)	862
AcsI	RAATTY	3 cut(s)	54, 837, 1113
AcuI	CTGAAG	2 cut(s)	107, 1515
AcvI	CACGTG	1 cut(s)	652
AcyI	GRCGYC	2 cut(s)	5, 105
AdeI	CACNNNGTG	1 cut(s)	422
AfaI	GTAC	5 cut(s)	385, 510, 1248, 1343, 1594
AfiI	CCNNNNNNNGG	5 cut(s)	24, 176, 248, 744, 1302
AflII	CTTAAG	1 cut(s)	1207
AflIII	ACRYGT	2 cut(s)	220, 649
AjnI	CCWGG	3 cut(s)	30, 60, 418
AjuI	GAANNNNNNNTTGG	2 cut(s)	971, 1003
Alw26I	GTCTC	3 cut(s)	12, 199, 1208
AlwI	GGATC	4 cut(s)	420, 433, 617, 630
AlwNI	CAGNNNCTG	1 cut(s)	1739
AoxI	GGCC	5 cut(s)	12, 178, 481, 862, 1019
ApoI	RAATTY	3 cut(s)	54, 837, 1113
Asp700I	GAANNNNTTC	1 cut(s)	1763
Asp718I	GGTACC	1 cut(s)	383
AspLEI	GCGC	1 cut(s)	107
AspS9I	GGNCC	2 cut(s)	28, 1019
AsuHPI	GGTGA	7 cut(s)	76, 166, 175, 890, 988, 1319, 1396
AsuII	TTCGAA	1 cut(s)	1148
AvaII	GGWCC	1 cut(s)	28
BalI	TGGCCA	1 cut(s)	864
BamHI	GGATCC	2 cut(s)	425, 622
BanI	GGYRCC	3 cut(s)	104, 311, 383
BanII	GRGCYC	1 cut(s)	1546
BbrPI	CACGTG	1 cut(s)	652
BbsI	GAAGAC	4 cut(s)	89, 102, 875, 1695
BccI	CCATC	3 cut(s)	95, 603, 1103
BceAI	ACGGC	2 cut(s)	176, 428
BcgI	CGANNNNNNTGC	2 cut(s)	293, 327
BciT130I	CCWGG	3 cut(s)	32, 62, 420
BciVI	GTATCC	1 cut(s)	592
BcoDI	GTCTC	3 cut(s)	12, 199, 1208
BfaI	CTAG	2 cut(s)	432, 618
BfmI	CTRYAG	2 cut(s)	450, 1131
BfoI	RGCGCY	1 cut(s)	108
BfrI	CTTAAG	1 cut(s)	1207
BfuAI	ACCTGC	1 cut(s)	520
BfuI	GTATCC	1 cut(s)	592
Bme1390I	CCNGG	3 cut(s)	32, 62, 420
Bme18I	GGWCC	1 cut(s)	28
BmgT120I	GGNCC	2 cut(s)	28, 1019
BmiI	GGNNCC	7 cut(s)	29, 106, 162, 313, 385, 427, 624
BmrFI	CCNGG	3 cut(s)	32, 62, 420
BmrI	ACTGGG	1 cut(s)	333
BmsI	GCATC	8 cut(s)	175, 253, 413, 537, 1040, 1158, 1315, 1650
BmuI	ACTGGG	1 cut(s)	333
BoxI	GACNNNNGTC	1 cut(s)	1269
BpiI	GAAGAC	4 cut(s)	89, 102, 875, 1695
BplI	GAGNNNNNCTC	2 cut(s)	1157, 1189
BpmI	CTGGAG	2 cut(s)	384, 1046
Bpu14I	TTCGAA	1 cut(s)	1148
BpuEI	CTTGAG	2 cut(s)	1530, 1733
BsaAI	YACGTR	1 cut(s)	652
BsaHI	GRCGYC	2 cut(s)	5, 105
BsaI	GGTCTC	1 cut(s)	1208
BsaJI	CCNNGG	5 cut(s)	156, 444, 1407, 1431, 1581
BsaXI	ACNNNNNCTCC	2 cut(s)	429, 459
Bsc4I	CCNNNNNNNGG	5 cut(s)	24, 176, 248, 744, 1302
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	7 cut(s)	232, 328, 367, 1029, 1326, 1472, 1768
BseBI	CCWGG	3 cut(s)	32, 62, 420
BseDI	CCNNGG	5 cut(s)	156, 444, 1407, 1431, 1581
BseGI	GGATG	9 cut(s)	166, 238, 346, 614, 893, 1031, 1111, 1330, 1388
BseLI	CCNNNNNNNGG	5 cut(s)	24, 176, 248, 744, 1302
BseMII	CTCAG	3 cut(s)	23, 765, 1167
BseNI	ACTGG	7 cut(s)	232, 328, 367, 1029, 1326, 1472, 1768
BseRI	GAGGAG	2 cut(s)	141, 1055
BseYI	CCCAGC	1 cut(s)	515
BsgI	GTGCAG	2 cut(s)	941, 1316
BshFI	GGCC	5 cut(s)	14, 180, 483, 864, 1021
BshNI	GGYRCC	3 cut(s)	104, 311, 383
BsiSI	CCGG	1 cut(s)	177
BslFI	GGGAC	2 cut(s)	231, 833
BslI	CCNNNNNNNGG	5 cut(s)	24, 176, 248, 744, 1302
BsmAI	GTCTC	3 cut(s)	12, 199, 1208
BsmBI	CGTCTC	2 cut(s)	12, 199
BsmFI	GGGAC	2 cut(s)	231, 833
BsnI	GGCC	5 cut(s)	14, 180, 483, 864, 1021
Bso31I	GGTCTC	1 cut(s)	1208
Bsp119I	TTCGAA	1 cut(s)	1148
Bsp1286I	GDGCHC	1 cut(s)	1546
Bsp1407I	TGTACA	1 cut(s)	1341
Bsp143I	GATC	4 cut(s)	425, 460, 622, 1309
BspACI	CCGC	8 cut(s)	15, 118, 137, 140, 164, 456, 1186, 1737
BspANI	GGCC	5 cut(s)	14, 180, 483, 864, 1021
BspCNI	CTCAG	3 cut(s)	22, 766, 1168
BspHI	TCATGA	1 cut(s)	1261
BspLI	GGNNCC	7 cut(s)	29, 106, 162, 313, 385, 427, 624
BspMI	ACCTGC	1 cut(s)	520
BspPI	GGATC	4 cut(s)	420, 433, 617, 630
BspT104I	TTCGAA	1 cut(s)	1148
BspT107I	GGYRCC	3 cut(s)	104, 311, 383
BspTI	CTTAAG	1 cut(s)	1207
BspTNI	GGTCTC	1 cut(s)	1208
BsrBI	CCGCTC	1 cut(s)	118
BsrFI	RCCGGY	1 cut(s)	176
BsrGI	TGTACA	1 cut(s)	1341
BsrI	ACTGG	7 cut(s)	232, 328, 367, 1029, 1326, 1472, 1768
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	5 cut(s)	156, 444, 1407, 1431, 1581
BssMI	GATC	4 cut(s)	425, 460, 622, 1309
BssNI	GRCGYC	2 cut(s)	5, 105
BssT1I	CCWWGG	3 cut(s)	156, 1407, 1431
Bst2UI	CCWGG	3 cut(s)	32, 62, 420
Bst4CI	ACNGT	7 cut(s)	334, 383, 493, 604, 1246, 1416, 1535
Bst6I	CTCTTC	1 cut(s)	1636
BstACI	GRCGYC	2 cut(s)	5, 105
BstAFI	CTTAAG	1 cut(s)	1207
BstAPI	GCANNNNNTGC	1 cut(s)	1739
BstAUI	TGTACA	1 cut(s)	1341
BstBAI	YACGTR	1 cut(s)	652
BstBI	TTCGAA	1 cut(s)	1148
BstC8I	GCNNGC	4 cut(s)	147, 909, 1219, 1362
BstDEI	CTNAG	5 cut(s)	9, 774, 815, 1176, 1508
BstDSI	CCRYGG	1 cut(s)	444
BstF5I	GGATG	9 cut(s)	166, 238, 346, 614, 893, 1031, 1111, 1330, 1388
BstH2I	RGCGCY	1 cut(s)	108
BstHHI	GCGC	1 cut(s)	107
BstKTI	GATC	4 cut(s)	428, 463, 625, 1312
BstMAI	GTCTC	3 cut(s)	12, 199, 1208
BstMBI	GATC	4 cut(s)	425, 460, 622, 1309
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 124, 446, 455, 1370, 1427, 1739, 1742
BstNI	CCWGG	3 cut(s)	32, 62, 420
BstNSI	RCATGY	3 cut(s)	224, 321, 1703
BstPAI	GACNNNNGTC	1 cut(s)	1269
BstSCI	CCNGG	3 cut(s)	30, 60, 418
BstSFI	CTRYAG	2 cut(s)	450, 1131
BstV2I	GAAGAC	4 cut(s)	89, 102, 875, 1695
BstX2I	RGATCY	2 cut(s)	425, 622
BstYI	RGATCY	2 cut(s)	425, 622
BsuI	GTATCC	1 cut(s)	592
BsuRI	GGCC	5 cut(s)	14, 180, 483, 864, 1021
BtgI	CCRYGG	1 cut(s)	444
BtgZI	GCGATG	1 cut(s)	264
BtsCI	GGATG	9 cut(s)	166, 238, 346, 614, 893, 1031, 1111, 1330, 1388
BtsI	GCAGTG	2 cut(s)	1328, 1647
BtsIMutI	CAGTG	5 cut(s)	321, 771, 1022, 1328, 1647
BveI	ACCTGC	1 cut(s)	520
Cac8I	GCNNGC	4 cut(s)	147, 909, 1219, 1362
CaiI	CAGNNNCTG	1 cut(s)	1739
CciI	TCATGA	1 cut(s)	1261
CfoI	GCGC	1 cut(s)	107
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	2 cut(s)	28, 1019
CseI	GACGC	1 cut(s)	626
CsiI	ACCWGGT	1 cut(s)	418
Csp6I	GTAC	5 cut(s)	384, 509, 1247, 1342, 1593
CspCI	CAANNNNNGTGG	2 cut(s)	1492, 1527
CviQI	GTAC	5 cut(s)	384, 509, 1247, 1342, 1593
DdeI	CTNAG	5 cut(s)	9, 774, 815, 1176, 1508
DinI	GGCGCC	1 cut(s)	106
DpnI	GATC	4 cut(s)	427, 462, 624, 1311
DpnII	GATC	4 cut(s)	425, 460, 622, 1309
DraIII	CACNNNGTG	1 cut(s)	422
EaeI	YGGCCR	1 cut(s)	862
Eam1104I	CTCTTC	1 cut(s)	1636
EarI	CTCTTC	1 cut(s)	1636
EciI	GGCGGA	1 cut(s)	126
Eco130I	CCWWGG	3 cut(s)	156, 1407, 1431
Eco24I	GRGCYC	1 cut(s)	1546
Eco31I	GGTCTC	1 cut(s)	1208
Eco47I	GGWCC	1 cut(s)	28
Eco57I	CTGAAG	2 cut(s)	107, 1515
Eco72I	CACGTG	1 cut(s)	652
EcoO109I	RGGNCCY	1 cut(s)	28
EcoRI	GAATTC	1 cut(s)	54
EcoRII	CCWGG	3 cut(s)	30, 60, 418
EcoT14I	CCWWGG	3 cut(s)	156, 1407, 1431
EcoT38I	GRGCYC	1 cut(s)	1546
EgeI	GGCGCC	1 cut(s)	106
EheI	GGCGCC	1 cut(s)	106
ErhI	CCWWGG	3 cut(s)	156, 1407, 1431
Esp3I	CGTCTC	2 cut(s)	12, 199
FalI	AAGNNNNNCTT	2 cut(s)	1539, 1571
FaqI	GGGAC	2 cut(s)	231, 833
FokI	GGATG	9 cut(s)	153, 225, 353, 621, 880, 1018, 1118, 1337, 1375
FriOI	GRGCYC	1 cut(s)	1546
FspBI	CTAG	2 cut(s)	432, 618
GlaI	GCGC	1 cut(s)	106
GsaI	CCCAGC	1 cut(s)	519
GsuI	CTGGAG	2 cut(s)	384, 1046
HaeII	RGCGCY	1 cut(s)	108
HaeIII	GGCC	5 cut(s)	14, 180, 483, 864, 1021
HapII	CCGG	1 cut(s)	177
HgaI	GACGC	1 cut(s)	626
HhaI	GCGC	1 cut(s)	107
Hin1I	GRCGYC	2 cut(s)	5, 105
Hin6I	GCGC	1 cut(s)	105
HinP1I	GCGC	1 cut(s)	105
HindIII	AAGCTT	3 cut(s)	779, 844, 1629
HinfI	GANTC	5 cut(s)	537, 733, 971, 992, 1569
HpaII	CCGG	1 cut(s)	177
HphI	GGTGA	7 cut(s)	76, 166, 175, 890, 988, 1319, 1396
Hpy166II	GTNNAC	1 cut(s)	1344
Hpy188I	TCNGA	9 cut(s)	92, 199, 276, 542, 947, 1036, 1177, 1495, 1640
Hpy188III	TCNNGA	7 cut(s)	242, 411, 596, 722, 1262, 1556, 1712
Hpy8I	GTNNAC	1 cut(s)	1344
Hpy99I	CGWCG	2 cut(s)	203, 642
HpyAV	CCTTC	6 cut(s)	196, 299, 1096, 1155, 1156, 1468
HpyCH4III	ACNGT	7 cut(s)	334, 383, 493, 604, 1246, 1416, 1535
HpyCH4IV	ACGT	3 cut(s)	201, 651, 1526
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 124, 446, 455, 1370, 1427, 1739, 1742
HpyF3I	CTNAG	5 cut(s)	9, 774, 815, 1176, 1508
HpySE526I	ACGT	3 cut(s)	201, 651, 1526
Hsp92I	GRCGYC	2 cut(s)	5, 105
HspAI	GCGC	1 cut(s)	105
KasI	GGCGCC	1 cut(s)	104
KpnI	GGTACC	1 cut(s)	387
Kzo9I	GATC	4 cut(s)	425, 460, 622, 1309
LmnI	GCTCC	4 cut(s)	154, 166, 437, 1357
LweI	GCATC	8 cut(s)	175, 253, 413, 537, 1040, 1158, 1315, 1650
MabI	ACCWGGT	1 cut(s)	418
MaeI	CTAG	2 cut(s)	432, 618
MaeII	ACGT	3 cut(s)	201, 651, 1526
MaeIII	GTNAC	4 cut(s)	64, 223, 1240, 1535
MalI	GATC	4 cut(s)	427, 462, 624, 1311
MbiI	CCGCTC	1 cut(s)	118
MboI	GATC	4 cut(s)	425, 460, 622, 1309
MboII	GAAGA	9 cut(s)	94, 107, 122, 264, 399, 880, 920, 1653, 1700
MflI	RGATCY	2 cut(s)	425, 622
MhlI	GDGCHC	1 cut(s)	1546
MlsI	TGGCCA	1 cut(s)	864
MluNI	TGGCCA	1 cut(s)	864
Mly113I	GGCGCC	1 cut(s)	105
MlyI	GAGTC	1 cut(s)	1001
MmeI	TCCRAC	3 cut(s)	222, 586, 625
Mox20I	TGGCCA	1 cut(s)	864
MroXI	GAANNNNTTC	1 cut(s)	1763
MscI	TGGCCA	1 cut(s)	864
MseI	TTAA	1 cut(s)	1208
MslI	CAYNNNNRTG	2 cut(s)	954, 1305
Msp20I	TGGCCA	1 cut(s)	864
MspA1I	CMGCKG	3 cut(s)	458, 1085, 1739
MspCI	CTTAAG	1 cut(s)	1207
MspI	CCGG	1 cut(s)	177
MspR9I	CCNGG	3 cut(s)	32, 62, 420
MvaI	CCWGG	3 cut(s)	32, 62, 420
MwoI	GCNNNNNNNGC	8 cut(s)	11, 124, 446, 455, 1370, 1427, 1739, 1742
NarI	GGCGCC	1 cut(s)	105
NdeII	GATC	4 cut(s)	425, 460, 622, 1309
NlaIV	GGNNCC	7 cut(s)	29, 106, 162, 313, 385, 427, 624
NmeAIII	GCCGAG	2 cut(s)	459, 1606
NmuCI	GTSAC	2 cut(s)	64, 1240
NspI	RCATGY	3 cut(s)	224, 321, 1703
NspV	TTCGAA	1 cut(s)	1148
PagI	TCATGA	1 cut(s)	1261
PciI	ACATGT	1 cut(s)	220
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PdmI	GAANNNNTTC	1 cut(s)	1763
PfeI	GAWTC	4 cut(s)	537, 733, 971, 1569
PflMI	CCANNNNNTGG	1 cut(s)	24
PleI	GAGTC	1 cut(s)	1000
PluTI	GGCGCC	1 cut(s)	108
PmaCI	CACGTG	1 cut(s)	652
PmlI	CACGTG	1 cut(s)	652
PpsI	GAGTC	1 cut(s)	1000
Ppu21I	YACGTR	1 cut(s)	652
PpuMI	RGGWCCY	1 cut(s)	28
PscI	ACATGT	1 cut(s)	220
PshAI	GACNNNNGTC	1 cut(s)	1269
Psp5II	RGGWCCY	1 cut(s)	28
Psp6I	CCWGG	3 cut(s)	30, 60, 418
PspCI	CACGTG	1 cut(s)	652
PspFI	CCCAGC	1 cut(s)	515
PspGI	CCWGG	3 cut(s)	30, 60, 418
PspN4I	GGNNCC	7 cut(s)	29, 106, 162, 313, 385, 427, 624
PspPI	GGNCC	2 cut(s)	28, 1019
PspPPI	RGGWCCY	1 cut(s)	28
PstNI	CAGNNNCTG	1 cut(s)	1739
PsuI	RGATCY	2 cut(s)	425, 622
PvuII	CAGCTG	1 cut(s)	1085
RsaI	GTAC	5 cut(s)	385, 510, 1248, 1343, 1594
RsaNI	GTAC	5 cut(s)	384, 509, 1247, 1342, 1593
RseI	CAYNNNNRTG	2 cut(s)	954, 1305
SaqAI	TTAA	1 cut(s)	1208
Sau3AI	GATC	4 cut(s)	425, 460, 622, 1309
Sau96I	GGNCC	2 cut(s)	28, 1019
SchI	GAGTC	1 cut(s)	1001
ScrFI	CCNGG	3 cut(s)	32, 62, 420
SduI	GDGCHC	1 cut(s)	1546
SexAI	ACCWGGT	1 cut(s)	418
SfaNI	GCATC	8 cut(s)	175, 253, 413, 537, 1040, 1158, 1315, 1650
SfcI	CTRYAG	2 cut(s)	450, 1131
SfoI	GGCGCC	1 cut(s)	106
SfuI	TTCGAA	1 cut(s)	1148
SinI	GGWCC	1 cut(s)	28
SmiMI	CAYNNNNRTG	2 cut(s)	954, 1305
SmlI	CTYRAG	3 cut(s)	1207, 1545, 1712
SmoI	CTYRAG	3 cut(s)	1207, 1545, 1712
SsiI	CCGC	8 cut(s)	15, 118, 137, 140, 164, 456, 1186, 1737
SspDI	GGCGCC	1 cut(s)	104
SspMI	CTAG	2 cut(s)	432, 618
StyD4I	CCNGG	3 cut(s)	30, 60, 418
StyI	CCWWGG	3 cut(s)	156, 1407, 1431
TaaI	ACNGT	7 cut(s)	334, 383, 493, 604, 1246, 1416, 1535
TaiI	ACGT	3 cut(s)	204, 654, 1529
TaqI	TCGA	3 cut(s)	303, 1148, 1447
TatI	WGTACW	2 cut(s)	1341, 1592
TauI	GCSGC	5 cut(s)	17, 121, 142, 458, 1739
TfiI	GAWTC	4 cut(s)	537, 733, 971, 1569
Tru1I	TTAA	1 cut(s)	1208
Tru9I	TTAA	1 cut(s)	1208
TscAI	CASTG	5 cut(s)	328, 778, 1029, 1335, 1654
TseFI	GTSAC	2 cut(s)	64, 1240
Tsp45I	GTSAC	2 cut(s)	64, 1240
TspDTI	ATGAA	8 cut(s)	357, 822, 848, 882, 1086, 1250, 1279, 1561
TspRI	CASTG	5 cut(s)	328, 778, 1029, 1335, 1654
Van91I	CCANNNNNTGG	1 cut(s)	24
Vha464I	CTTAAG	1 cut(s)	1207
VpaK11BI	GGWCC	1 cut(s)	28
XapI	RAATTY	3 cut(s)	54, 837, 1113
XceI	RCATGY	3 cut(s)	224, 321, 1703
XcmI	CCANNNNNNNNNTGG	1 cut(s)	872
XmnI	GAANNNNTTC	1 cut(s)	1763
XspI	CTAG	2 cut(s)	432, 618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.