Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012253

Physical Location & Seq

Genomic Coordinates Reverse (-)

31652794 .. 31658376

Gene Structure 5583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1236500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1773 bp

ATGGGGTCTCAGGCCGCCAATCTGTGGGTTCTGCTGGGTTTAGGTTTGGCTGGAATTCTCCTGATGACAAGGAAGCTGAAGAAAACCATAAGAGAAGACTTTGGCGCCTTTGTTGAGCGGCTTCAGCTTCTTCCTCCGCCGCAGCCTGCTCCTCCCAAGGCTCCACACCCCCTCACCGGCCTCACCTTCGCTGTCTCCGACGTATTTGAAATTGAGGGACATGTAACTGGTTTTGGTCATCCTGATTGGGGAAGAACACATGATGCGGCTTCCCGGACGTGTCCTGTGGTTTCCACTCTTGTCGAAGGAGGTGCCACATGCACTGGGATAACTGTTGTGGATGAATTGGCATATAGTATTACTGGAGAAAATAAGCATTATGGTACCCCCACCAATCCAGCAGCATCTTCAAGAAAACCAGGTGGATCCTCTAGTGGAGCTGCTGTGGCTGTAGCCGCTGATCTTGTTGACTTTTCACTGGGTCTTGATACTGTTGGTGGTGTTAGAGTACCTGCTGGGTCTTGTGGCATCATTGGATTCCGAGCTTCACATGGCTCTGTTTCTCATGCTGGATTTATACCTGTTTCTACAAGTCTGGATACTGTTGGATGGTTTGCTAGGGATCCTAATGTCCTGCGTCGTGTTGGACATGTGCTGCTGCAACTTCCTTATGCAGTTTCACGCAGTCCAAGACAAATTGTAATAGCTGATGATTGCTTTCAAGAAGTAAAGATTCCAGTTGATAGGATTGTTCAAGTGGTTATCAAATCCACTGAGAAGCTTTTTGGAAAGCAAGTGTTGAAACATGAAAATATTGGGGACTACTGCAATTCAAAAGTTCCAAGCTTGAAAAAGTTTCATGGCCTGAAGACAAATGGTGAAGTAAAGACTTCATCCCTCAAATTGCTTGCCAATGTTGTGCAGTTTCTTCAAAGGCATGAGTTCAGACATAATCATGGGGAGTGGATTGATTCGGTAAAGCCTGTTTTGGAGTCTGCTACTTCAGCGCAAATATGTGGGCCACTGGAGGCATCCGATGAGGAGATTGAGATATGCAAGTCAATTAGGAATGAATTACGGGCAGCTGTCAGCTCTCTATTGAAGGATGATGGAATTTTGGTTATCCCAACTATAGCAGACCCCCCTGCAAAACTTGGGGGGAAGGAGATGCTCTCTGAGGACTTCCGGAATCGTACTTTTGGTCTCTTAAGTATTGCAAGCCTGTCAGGCTGTTGTCAGGTCACAGCACCTCTGGGATTTTATGACAAGTGCCCTGTTTCAGTTTCTTTCATAGCCAGGCACGGGGGTGATCGCTTTTTACTGGATGCACTACACACTATGTATATGTCTTTACAGGAGCAGGCCGATATTGCTTCAAAATCCAGATCATCCAAAAATGCTCTCACCAAGGAACAATCTGCTGAAATTGCCAAGGAAAAGGGAAATCAAGCATACAAAGATAGAGAGTGGCAGAAGGCTATTGGCTTTTATTCAGAAGCTATCAAACTTAGTGGCAACAATGCAACGTATTACAGTAACAGGGCTCAAGCATATCTTGAAGTTGGAAGTTTCATTCAAGCGGAGGCAGATTGTACAAAAGCCATCAACCTTGACAAAAAGAATGTGAAAGCTTATTTCCGAAGAGGCACTGCAAGGGAGATGCTGGGTTACTATAAGGAAGCGATTGAAGACTTTAGACACACACTTGTTCTTGAGCCAACCAACAAACGAGCAGCCGTTGCTGCTGAAAAATTGAGGAAGCTGTTCCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0016020 GO:0016021 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase TPR_11 TPR_16 TPR_2 TPR_8

Protein Analysis

591

Amino Acids

64.09

Weight (kDa)

8.77

Isoelectric Point (pI)

37.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	55 - 218	5.2e-38	Amidase
TPR_BSK1_C	PF25575	441 - 541	5.9e-06	Serine/threonine-protein kinase BSK1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012253)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17970 AT3G17970
fragaria_vesca	FvH4_5g17380
malus_domestica	MD06G1227100.v1.1 MD14G1236500.v1.1
prunus_persica	Prupe.5G233600_v2.0.a1
pyrus_communis	pycom06g20340 pycom14g19750
rosa_chinensis	RchiOBHm_Chr7g0178361
rosa_laevigata	RLG00000005452
rosa_roxburghii	Rroxscaffold_3G00274980
rosa_rugosa	Rorug06G0416300 Rorug06G0416400
rosa_samantha	Rh7AG015100 Rh7BG014600 Rh7CG014600 Rh7DG014900
rosa_wichuraiana	Rw0G010200 Rw7G001200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	520
Acc65I	GGTACC	1 cut(s)	383
AccB1I	GGYRCC	3 cut(s)	104, 311, 383
AccB7I	CCANNNNNTGG	1 cut(s)	24
AccBSI	CCGCTC	1 cut(s)	118
AccIII	TCCGGA	1 cut(s)	1185
AciI	CCGC	7 cut(s)	15, 118, 137, 140, 266, 456, 1580
AclWI	GGATC	4 cut(s)	420, 433, 617, 630
AcsI	RAATTY	2 cut(s)	54, 1113
AcuI	CTGAAG	4 cut(s)	98, 107, 887, 987
AcyI	GRCGYC	1 cut(s)	105
AfaI	GTAC	4 cut(s)	385, 510, 1195, 1594
AfiI	CCNNNNNNNGG	4 cut(s)	24, 176, 248, 1302
AflII	CTTAAG	1 cut(s)	1207
AflIII	ACRYGT	3 cut(s)	220, 278, 649
AjiI	CACGTC	1 cut(s)	279
AjnI	CCWGG	2 cut(s)	418, 1295
AleI	CACNNNNGTG	1 cut(s)	1305
Alw26I	GTCTC	3 cut(s)	12, 199, 1208
AlwI	GGATC	4 cut(s)	420, 433, 617, 630
Aor13HI	TCCGGA	1 cut(s)	1185
AoxI	GGCC	5 cut(s)	12, 178, 862, 1019, 1362
ApeKI	GCWGC	8 cut(s)	142, 401, 440, 655, 658, 1082, 1733, 1742
ApoI	RAATTY	2 cut(s)	54, 1113
Asp700I	GAANNNNTTC	1 cut(s)	1763
Asp718I	GGTACC	1 cut(s)	383
AspLEI	GCGC	2 cut(s)	107, 1009
AspS9I	GGNCC	1 cut(s)	1019
AsuC2I	CCSGG	1 cut(s)	274
AsuHPI	GGTGA	5 cut(s)	166, 175, 890, 1319, 1396
BaeGI	GKGCMC	1 cut(s)	1274
BamHI	GGATCC	2 cut(s)	425, 622
BanI	GGYRCC	3 cut(s)	104, 311, 383
BanII	GRGCYC	1 cut(s)	1546
BbsI	GAAGAC	3 cut(s)	102, 875, 1695
BbvI	GCAGC	8 cut(s)	154, 413, 427, 642, 645, 1094, 1729, 1745
BccI	CCATC	3 cut(s)	603, 1103, 1610
BceAI	ACGGC	1 cut(s)	1721
BcgI	CGANNNNNNTGC	2 cut(s)	293, 327
BciT130I	CCWGG	2 cut(s)	420, 1297
BciVI	GTATCC	1 cut(s)	592
BcnI	CCSGG	1 cut(s)	274
BcoDI	GTCTC	3 cut(s)	12, 199, 1208
BfaI	CTAG	2 cut(s)	432, 618
BfmI	CTRYAG	3 cut(s)	450, 1131, 1769
BfoI	RGCGCY	1 cut(s)	108
BfrI	CTTAAG	1 cut(s)	1207
BfuAI	ACCTGC	1 cut(s)	520
BfuI	GTATCC	1 cut(s)	592
BglI	GCCNNNNNGGC	1 cut(s)	1227
Bme1390I	CCNGG	3 cut(s)	274, 420, 1297
BmgBI	CACGTC	1 cut(s)	279
BmgT120I	GGNCC	1 cut(s)	1019
BmiI	GGNNCC	6 cut(s)	106, 162, 313, 385, 427, 624
BmrFI	CCNGG	3 cut(s)	274, 420, 1297
BmrI	ACTGGG	2 cut(s)	333, 488
BmsI	GCATC	7 cut(s)	253, 413, 537, 1040, 1158, 1315, 1650
BmuI	ACTGGG	2 cut(s)	333, 488
BpiI	GAAGAC	3 cut(s)	102, 875, 1695
BplI	GAGNNNNNCTC	2 cut(s)	1157, 1189
BpmI	CTGGAG	2 cut(s)	384, 1046
BpuEI	CTTGAG	2 cut(s)	1530, 1733
BpuMI	CCSGG	1 cut(s)	274
BsaHI	GRCGYC	1 cut(s)	105
BsaI	GGTCTC	2 cut(s)	12, 1208
BsaJI	CCNNGG	3 cut(s)	156, 1407, 1431
BsaWI	WCCGGW	1 cut(s)	1185
BsaXI	ACNNNNNCTCC	2 cut(s)	429, 459
Bsc4I	CCNNNNNNNGG	4 cut(s)	24, 176, 248, 1302
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	7 cut(s)	232, 328, 367, 483, 737, 1029, 1326
BseAI	TCCGGA	1 cut(s)	1185
BseBI	CCWGG	2 cut(s)	420, 1297
BseDI	CCNNGG	3 cut(s)	156, 1407, 1431
BseGI	GGATG	8 cut(s)	238, 346, 614, 893, 1031, 1111, 1330, 1388
BseLI	CCNNNNNNNGG	4 cut(s)	24, 176, 248, 1302
BseMII	CTCAG	3 cut(s)	23, 765, 1167
BseNI	ACTGG	7 cut(s)	232, 328, 367, 483, 737, 1029, 1326
BseRI	GAGGAG	2 cut(s)	141, 1055
BseSI	GKGCMC	1 cut(s)	1274
BseXI	GCAGC	8 cut(s)	154, 413, 427, 642, 645, 1094, 1729, 1745
BseYI	CCCAGC	3 cut(s)	34, 515, 1663
BsgI	GTGCAG	1 cut(s)	941
BshFI	GGCC	5 cut(s)	14, 180, 864, 1021, 1364
BshNI	GGYRCC	3 cut(s)	104, 311, 383
BsiSI	CCGG	3 cut(s)	177, 274, 1186
BslFI	GGGAC	2 cut(s)	231, 833
BslI	CCNNNNNNNGG	4 cut(s)	24, 176, 248, 1302
BsmAI	GTCTC	3 cut(s)	12, 199, 1208
BsmFI	GGGAC	2 cut(s)	231, 833
BsnI	GGCC	5 cut(s)	14, 180, 864, 1021, 1364
Bso31I	GGTCTC	2 cut(s)	12, 1208
Bsp1286I	GDGCHC	2 cut(s)	1274, 1546
Bsp13I	TCCGGA	1 cut(s)	1185
Bsp1407I	TGTACA	1 cut(s)	1592
Bsp143I	GATC	5 cut(s)	425, 460, 622, 1309, 1385
BspACI	CCGC	7 cut(s)	15, 118, 137, 140, 266, 456, 1580
BspANI	GGCC	5 cut(s)	14, 180, 864, 1021, 1364
BspCNI	CTCAG	3 cut(s)	22, 766, 1168
BspEI	TCCGGA	1 cut(s)	1185
BspLI	GGNNCC	6 cut(s)	106, 162, 313, 385, 427, 624
BspMI	ACCTGC	1 cut(s)	520
BspPI	GGATC	4 cut(s)	420, 433, 617, 630
BspT107I	GGYRCC	3 cut(s)	104, 311, 383
BspTI	CTTAAG	1 cut(s)	1207
BspTNI	GGTCTC	2 cut(s)	12, 1208
BsrBI	CCGCTC	1 cut(s)	118
BsrFI	RCCGGY	1 cut(s)	176
BsrGI	TGTACA	1 cut(s)	1592
BsrI	ACTGG	7 cut(s)	232, 328, 367, 483, 737, 1029, 1326
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	3 cut(s)	156, 1407, 1431
BssMI	GATC	5 cut(s)	425, 460, 622, 1309, 1385
BssNI	GRCGYC	1 cut(s)	105
BssT1I	CCWWGG	3 cut(s)	156, 1407, 1431
Bst2UI	CCWGG	2 cut(s)	420, 1297
Bst4CI	ACNGT	4 cut(s)	334, 493, 604, 1535
Bst6I	CTCTTC	1 cut(s)	1636
BstACI	GRCGYC	1 cut(s)	105
BstAFI	CTTAAG	1 cut(s)	1207
BstAPI	GCANNNNNTGC	1 cut(s)	1739
BstAUI	TGTACA	1 cut(s)	1592
BstC8I	GCNNGC	4 cut(s)	147, 909, 1219, 1362
BstDEI	CTNAG	4 cut(s)	9, 774, 1176, 1508
BstF5I	GGATG	8 cut(s)	238, 346, 614, 893, 1031, 1111, 1330, 1388
BstH2I	RGCGCY	1 cut(s)	108
BstHHI	GCGC	2 cut(s)	107, 1009
BstKTI	GATC	5 cut(s)	428, 463, 625, 1312, 1388
BstMAI	GTCTC	3 cut(s)	12, 199, 1208
BstMBI	GATC	5 cut(s)	425, 460, 622, 1309, 1385
BstMWI	GCNNNNNNNGC	9 cut(s)	124, 446, 455, 1004, 1227, 1370, 1427, 1739, 1742
BstNI	CCWGG	2 cut(s)	420, 1297
BstNSI	RCATGY	3 cut(s)	224, 321, 653
BstSCI	CCNGG	3 cut(s)	272, 418, 1295
BstSFI	CTRYAG	3 cut(s)	450, 1131, 1769
BstSLI	GKGCMC	1 cut(s)	1274
BstV1I	GCAGC	8 cut(s)	154, 413, 427, 642, 645, 1094, 1729, 1745
BstV2I	GAAGAC	3 cut(s)	102, 875, 1695
BstX2I	RGATCY	2 cut(s)	425, 622
BstYI	RGATCY	2 cut(s)	425, 622
BsuI	GTATCC	1 cut(s)	592
BsuRI	GGCC	5 cut(s)	14, 180, 864, 1021, 1364
BtrI	CACGTC	1 cut(s)	279
BtsCI	GGATG	8 cut(s)	238, 346, 614, 893, 1031, 1111, 1330, 1388
BtsI	GCAGTG	1 cut(s)	1647
BtsIMutI	CAGTG	5 cut(s)	321, 476, 771, 1022, 1647
BveI	ACCTGC	1 cut(s)	520
Cac8I	GCNNGC	4 cut(s)	147, 909, 1219, 1362
CfoI	GCGC	2 cut(s)	107, 1009
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	1 cut(s)	1019
CseI	GACGC	1 cut(s)	626
CsiI	ACCWGGT	1 cut(s)	418
Csp6I	GTAC	4 cut(s)	384, 509, 1194, 1593
CspCI	CAANNNNNGTGG	2 cut(s)	1492, 1527
CviQI	GTAC	4 cut(s)	384, 509, 1194, 1593
DdeI	CTNAG	4 cut(s)	9, 774, 1176, 1508
DinI	GGCGCC	1 cut(s)	106
DpnI	GATC	5 cut(s)	427, 462, 624, 1311, 1387
DpnII	GATC	5 cut(s)	425, 460, 622, 1309, 1385
Eam1104I	CTCTTC	1 cut(s)	1636
EarI	CTCTTC	1 cut(s)	1636
EciI	GGCGGA	1 cut(s)	126
Eco130I	CCWWGG	3 cut(s)	156, 1407, 1431
Eco24I	GRGCYC	1 cut(s)	1546
Eco31I	GGTCTC	2 cut(s)	12, 1208
Eco57I	CTGAAG	4 cut(s)	98, 107, 887, 987
EcoRI	GAATTC	1 cut(s)	54
EcoRII	CCWGG	2 cut(s)	418, 1295
EcoT14I	CCWWGG	3 cut(s)	156, 1407, 1431
EcoT38I	GRGCYC	1 cut(s)	1546
EgeI	GGCGCC	1 cut(s)	106
EheI	GGCGCC	1 cut(s)	106
ErhI	CCWWGG	3 cut(s)	156, 1407, 1431
FalI	AAGNNNNNCTT	2 cut(s)	1539, 1571
FaqI	GGGAC	2 cut(s)	231, 833
FokI	GGATG	8 cut(s)	225, 353, 621, 880, 1018, 1118, 1337, 1375
FriOI	GRGCYC	1 cut(s)	1546
FspBI	CTAG	2 cut(s)	432, 618
GlaI	GCGC	2 cut(s)	106, 1008
GsaI	CCCAGC	3 cut(s)	38, 519, 1667
GsuI	CTGGAG	2 cut(s)	384, 1046
HaeII	RGCGCY	1 cut(s)	108
HaeIII	GGCC	5 cut(s)	14, 180, 864, 1021, 1364
HapII	CCGG	3 cut(s)	177, 274, 1186
HgaI	GACGC	1 cut(s)	626
HhaI	GCGC	2 cut(s)	107, 1009
Hin1I	GRCGYC	1 cut(s)	105
Hin6I	GCGC	2 cut(s)	105, 1007
HinP1I	GCGC	2 cut(s)	105, 1007
HincII	GTYRAC	1 cut(s)	469
HindII	GTYRAC	1 cut(s)	469
HindIII	AAGCTT	3 cut(s)	779, 844, 1629
HinfI	GANTC	5 cut(s)	537, 733, 971, 992, 1189
HpaII	CCGG	3 cut(s)	177, 274, 1186
HphI	GGTGA	5 cut(s)	166, 175, 890, 1319, 1396
Hpy166II	GTNNAC	1 cut(s)	469
Hpy188I	TCNGA	7 cut(s)	199, 542, 947, 1036, 1177, 1495, 1640
Hpy8I	GTNNAC	1 cut(s)	469
Hpy99I	CGWCG	2 cut(s)	203, 642
HpyAV	CCTTC	5 cut(s)	196, 299, 1096, 1156, 1468
HpyCH4III	ACNGT	4 cut(s)	334, 493, 604, 1535
HpyCH4IV	ACGT	3 cut(s)	201, 278, 1526
HpyF10VI	GCNNNNNNNGC	9 cut(s)	124, 446, 455, 1004, 1227, 1370, 1427, 1739, 1742
HpyF3I	CTNAG	4 cut(s)	9, 774, 1176, 1508
HpySE526I	ACGT	3 cut(s)	201, 278, 1526
Hsp92I	GRCGYC	1 cut(s)	105
HspAI	GCGC	2 cut(s)	105, 1007
KasI	GGCGCC	1 cut(s)	104
Kpn2I	TCCGGA	1 cut(s)	1185
KpnI	GGTACC	1 cut(s)	387
Kzo9I	GATC	5 cut(s)	425, 460, 622, 1309, 1385
LmnI	GCTCC	4 cut(s)	154, 166, 437, 1357
Lsp1109I	GCAGC	8 cut(s)	154, 413, 427, 642, 645, 1094, 1729, 1745
LweI	GCATC	7 cut(s)	253, 413, 537, 1040, 1158, 1315, 1650
MabI	ACCWGGT	1 cut(s)	418
MaeI	CTAG	2 cut(s)	432, 618
MaeII	ACGT	3 cut(s)	201, 278, 1526
MaeIII	GTNAC	4 cut(s)	223, 1240, 1535, 1667
MalI	GATC	5 cut(s)	427, 462, 624, 1311, 1387
MbiI	CCGCTC	1 cut(s)	118
MboI	GATC	5 cut(s)	425, 460, 622, 1309, 1385
MboII	GAAGA	9 cut(s)	91, 107, 122, 264, 399, 880, 920, 1653, 1700
MflI	RGATCY	2 cut(s)	425, 622
MhlI	GDGCHC	2 cut(s)	1274, 1546
Mly113I	GGCGCC	1 cut(s)	105
MlyI	GAGTC	1 cut(s)	1001
MmeI	TCCRAC	4 cut(s)	222, 586, 625, 1543
MroI	TCCGGA	1 cut(s)	1185
MroXI	GAANNNNTTC	1 cut(s)	1763
MseI	TTAA	1 cut(s)	1208
MslI	CAYNNNNRTG	2 cut(s)	954, 1305
MspA1I	CMGCKG	2 cut(s)	458, 1085
MspCI	CTTAAG	1 cut(s)	1207
MspI	CCGG	3 cut(s)	177, 274, 1186
MspR9I	CCNGG	3 cut(s)	274, 420, 1297
MvaI	CCWGG	2 cut(s)	420, 1297
MwoI	GCNNNNNNNGC	9 cut(s)	124, 446, 455, 1004, 1227, 1370, 1427, 1739, 1742
NarI	GGCGCC	1 cut(s)	105
NciI	CCSGG	1 cut(s)	274
NdeII	GATC	5 cut(s)	425, 460, 622, 1309, 1385
NlaIV	GGNNCC	6 cut(s)	106, 162, 313, 385, 427, 624
NmuCI	GTSAC	1 cut(s)	1240
NspI	RCATGY	3 cut(s)	224, 321, 653
OliI	CACNNNNGTG	1 cut(s)	1305
PciI	ACATGT	2 cut(s)	220, 649
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PdmI	GAANNNNTTC	1 cut(s)	1763
PfeI	GAWTC	4 cut(s)	537, 733, 971, 1189
PflMI	CCANNNNNTGG	1 cut(s)	24
PfoI	TCCNGGA	1 cut(s)	272
PleI	GAGTC	1 cut(s)	1000
PluTI	GGCGCC	1 cut(s)	108
PpsI	GAGTC	1 cut(s)	1000
PscI	ACATGT	2 cut(s)	220, 649
Psp6I	CCWGG	2 cut(s)	418, 1295
PspFI	CCCAGC	3 cut(s)	34, 515, 1663
PspGI	CCWGG	2 cut(s)	418, 1295
PspN4I	GGNNCC	6 cut(s)	106, 162, 313, 385, 427, 624
PspPI	GGNCC	1 cut(s)	1019
PsuI	RGATCY	2 cut(s)	425, 622
PvuII	CAGCTG	1 cut(s)	1085
RsaI	GTAC	4 cut(s)	385, 510, 1195, 1594
RsaNI	GTAC	4 cut(s)	384, 509, 1194, 1593
RseI	CAYNNNNRTG	2 cut(s)	954, 1305
SaqAI	TTAA	1 cut(s)	1208
Sau3AI	GATC	5 cut(s)	425, 460, 622, 1309, 1385
Sau96I	GGNCC	1 cut(s)	1019
SchI	GAGTC	1 cut(s)	1001
ScrFI	CCNGG	3 cut(s)	274, 420, 1297
SduI	GDGCHC	2 cut(s)	1274, 1546
SexAI	ACCWGGT	1 cut(s)	418
SfaNI	GCATC	7 cut(s)	253, 413, 537, 1040, 1158, 1315, 1650
SfcI	CTRYAG	3 cut(s)	450, 1131, 1769
SfoI	GGCGCC	1 cut(s)	106
SmiMI	CAYNNNNRTG	2 cut(s)	954, 1305
SmlI	CTYRAG	3 cut(s)	1207, 1545, 1712
SmoI	CTYRAG	3 cut(s)	1207, 1545, 1712
SsiI	CCGC	7 cut(s)	15, 118, 137, 140, 266, 456, 1580
SspDI	GGCGCC	1 cut(s)	104
SspI	AATATT	1 cut(s)	814
SspMI	CTAG	2 cut(s)	432, 618
StyD4I	CCNGG	3 cut(s)	272, 418, 1295
StyI	CCWWGG	3 cut(s)	156, 1407, 1431
TaaI	ACNGT	4 cut(s)	334, 493, 604, 1535
TaiI	ACGT	3 cut(s)	204, 281, 1529
TaqI	TCGA	1 cut(s)	303
TatI	WGTACW	1 cut(s)	1592
TauI	GCSGC	5 cut(s)	17, 121, 142, 269, 458
TfiI	GAWTC	4 cut(s)	537, 733, 971, 1189
Tru1I	TTAA	1 cut(s)	1208
Tru9I	TTAA	1 cut(s)	1208
TscAI	CASTG	5 cut(s)	328, 483, 778, 1029, 1654
TseFI	GTSAC	1 cut(s)	1240
TseI	GCWGC	8 cut(s)	142, 401, 440, 655, 658, 1082, 1733, 1742
Tsp45I	GTSAC	1 cut(s)	1240
TspDTI	ATGAA	7 cut(s)	357, 822, 848, 882, 1086, 1279, 1561
TspRI	CASTG	5 cut(s)	328, 483, 778, 1029, 1654
Van91I	CCANNNNNTGG	1 cut(s)	24
Vha464I	CTTAAG	1 cut(s)	1207
XapI	RAATTY	2 cut(s)	54, 1113
XceI	RCATGY	3 cut(s)	224, 321, 653
XmnI	GAANNNNTTC	1 cut(s)	1763
XspI	CTAG	2 cut(s)	432, 618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.