AT3G23490

Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013313
Physical Location & Seq
Reverse (-)
8422649 .. 8424652
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G23490.3

Sequence Viewer

Length: 507 bp
ATGGAAGCGGCGAAGAAACAGAGTGTTACAAATCAGCTTCTCGCCGTGAAATCAGCTTCCGGCAAGACTTTTAGCCAGTTAGCCGCGGAGACAGGTCTAACCAACGTATACGTAGCTCAGCTTCTCCGTCGTCAAGCTCAGCTCAAACCGGACACAGTCCCAAAGCTTAAGGAAGCTTTACCAGCTCTGACCGATGAACTAATCGGAGATATGATGTCTCCTCCGTGGAGATCCTATGATCCTAATCTCATCCAAGAACCCACTATCTACAGGTTGAATGAAGCAGTGATGCATTTTGGTGAGAGTATAAAGGAGATTATCAATGAAGATTTTGGAGATGGCATCATGTCGGCGATAGATTTCTATTGCTCTGTCGACAAAATCAAAGGAGTGGATGGTAACAATCGCGTGGTTGTGACGCTTGATGGAAAGTATCTTTCGCATTCCGAACAGAGGACGGAGAATATGGTCTCAAGGCTAAATCTCAAGGGAGGTACAAGCGAATGA

Protein Analysis

168

Amino Acids

18.59

Weight (kDa)

5.49

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyanate_lyase PF02560 82 - 116 2e-13 Cyanate lyase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23490 AT3G23490 AT3G23490
fragaria_vesca FvH4_4g04220 FvH4_4g04220
malus_domestica MD13G1218700.v1.1
prunus_persica Prupe.1G045500_v2.0.a1
pyrus_communis pycom13g19370
rosa_chinensis RchiOBHm_Chr4g0394701
rosa_laevigata RLG00000009671
rosa_multiflora Rmu_sc0005356.1_g000020 Rmu_sc0005424.1_g000001
rosa_roxburghii Rroxscaffold_5G00339710
rosa_samantha Rh4AG054400 Rh4BG052100 Rh4CG059000 Rh4DG050700
rosa_wichuraiana Rw4G004340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 108, 375
AccII CGCG 2 cut(s) 86, 408
AciI CCGC 3 cut(s) 8, 84, 86
AclWI GGATC 2 cut(s) 225, 233
AfaI GTAC 1 cut(s) 496
AfiI CCNNNNNNNGG 1 cut(s) 453
AflII CTTAAG 1 cut(s) 167
AgsI TTSAA 1 cut(s) 277
AluBI AGCT 9 cut(s) 37, 56, 116, 121, 137, 142, 166, 176, 185
AluI AGCT 9 cut(s) 37, 56, 116, 121, 137, 142, 166, 176, 185
Alw26I GTCTC 3 cut(s) 83, 222, 475
AlwI GGATC 2 cut(s) 225, 233
AsuHPI GGTGA 1 cut(s) 311
BarI GAAGNNNNNNTAC 2 cut(s) 105, 137
BccI CCATC 3 cut(s) 332, 389, 419
BceAI ACGGC 1 cut(s) 29
BcoDI GTCTC 3 cut(s) 83, 222, 475
BfmI CTRYAG 1 cut(s) 268
BfrI CTTAAG 1 cut(s) 167
BisI GCNGC 2 cut(s) 9, 84
BlpI GCTNAGC 2 cut(s) 117, 138
BlsI GCNGC 2 cut(s) 10, 85
BmsI GCATC 2 cut(s) 279, 351
Bpu1102I GCTNAGC 2 cut(s) 117, 138
BpuEI CTTGAG 2 cut(s) 457, 470
BsaAI YACGTR 1 cut(s) 112
BsaBI GATNNNNATC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 2 cut(s) 84, 224
BsaWI WCCGGW 1 cut(s) 148
Bsc4I CCNNNNNNNGG 1 cut(s) 453
Bse1I ACTGG 1 cut(s) 76
Bse8I GATNNNNATC 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 84, 224
BseGI GGATG 2 cut(s) 249, 400
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 1 cut(s) 453
BseMII CTCAG 2 cut(s) 131, 152
BseNI ACTGG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 210
Bsh1236I CGCG 2 cut(s) 86, 408
BsiSI CCGG 2 cut(s) 60, 149
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 453
BsmAI GTCTC 3 cut(s) 83, 222, 475
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 442
Bso31I GGTCTC 1 cut(s) 475
Bsp143I GATC 2 cut(s) 230, 238
Bsp1720I GCTNAGC 2 cut(s) 117, 138
BspACI CCGC 3 cut(s) 8, 84, 86
BspCNI CTCAG 2 cut(s) 130, 151
BspFNI CGCG 2 cut(s) 86, 408
BspPI GGATC 2 cut(s) 225, 233
BspTI CTTAAG 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 475
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 2 cut(s) 84, 224
BssMI GATC 2 cut(s) 230, 238
BssNAI GTATAC 1 cut(s) 109
Bst1107I GTATAC 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 157
BstAFI CTTAAG 1 cut(s) 167
BstBAI YACGTR 1 cut(s) 112
BstDEI CTNAG 2 cut(s) 117, 138
BstDSI CCRYGG 2 cut(s) 84, 224
BstF5I GGATG 2 cut(s) 249, 400
BstFNI CGCG 2 cut(s) 86, 408
BstKTI GATC 2 cut(s) 233, 241
BstMAI GTCTC 3 cut(s) 83, 222, 475
BstMBI GATC 2 cut(s) 230, 238
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstSFI CTRYAG 1 cut(s) 268
BstSNI TACGTA 1 cut(s) 112
BstUI CGCG 2 cut(s) 86, 408
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BstZ17I GTATAC 1 cut(s) 109
BtgI CCRYGG 2 cut(s) 84, 224
BtsCI GGATG 2 cut(s) 249, 400
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 291
Cfr42I CCGCGG 1 cut(s) 87
CseI GACGC 1 cut(s) 427
Csp6I GTAC 1 cut(s) 495
CviAII CATG 1 cut(s) 346
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 2 cut(s) 117, 138
DpnI GATC 2 cut(s) 232, 240
DpnII GATC 2 cut(s) 230, 238
Eco105I TACGTA 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 294
FaeI CATG 1 cut(s) 349
FaiI YATR 6 cut(s) 109, 212, 237, 308, 347, 467
FaqI GGGAC 1 cut(s) 143
FatI CATG 1 cut(s) 345
FblI GTMKAC 2 cut(s) 108, 375
Fnu4HI GCNGC 2 cut(s) 9, 84
FokI GGATG 2 cut(s) 236, 407
Fsp4HI GCNGC 2 cut(s) 9, 84
GluI GCNGC 2 cut(s) 9, 84
HapII CCGG 2 cut(s) 60, 149
HgaI GACGC 1 cut(s) 427
Hin1II CATG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 2 cut(s) 164, 174
HpaII CCGG 2 cut(s) 60, 149
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 109, 376
Hpy188I TCNGA 3 cut(s) 189, 206, 448
Hpy8I GTNNAC 2 cut(s) 109, 376
Hpy99I CGWCG 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 2 cut(s) 105, 111
HpyCH4V TGCA 1 cut(s) 292
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 2 cut(s) 117, 138
HpySE526I ACGT 2 cut(s) 105, 111
Hsp92II CATG 1 cut(s) 349
KspI CCGCGG 1 cut(s) 87
Kzo9I GATC 2 cut(s) 230, 238
LpnPI CCDG 6 cut(s) 73, 78, 89, 162, 195, 256
LweI GCATC 2 cut(s) 279, 351
MaeII ACGT 2 cut(s) 105, 111
MaeIII GTNAC 3 cut(s) 25, 398, 415
MalI GATC 2 cut(s) 232, 240
MboI GATC 2 cut(s) 230, 238
MboII GAAGA 2 cut(s) 25, 338
MflI RGATCY 1 cut(s) 230
MnlI CCTC 3 cut(s) 231, 447, 485
Mph1103I ATGCAT 1 cut(s) 294
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 86
MspCI CTTAAG 1 cut(s) 167
MspI CCGG 2 cut(s) 60, 149
Mva1269I GAATGC 1 cut(s) 442
MvnI CGCG 2 cut(s) 86, 408
MwoI GCNNNNNNNGC 1 cut(s) 182
NdeII GATC 2 cut(s) 230, 238
NlaIII CATG 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 415
NsiI ATGCAT 1 cut(s) 294
PctI GAATGC 1 cut(s) 442
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 2 cut(s) 10, 85
Ppu21I YACGTR 1 cut(s) 112
PsuI RGATCY 1 cut(s) 230
PsyI GACNNNGTC 1 cut(s) 155
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
RseI CAYNNNNRTG 1 cut(s) 297
SacII CCGCGG 1 cut(s) 87
SalI GTCGAC 1 cut(s) 374
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 2 cut(s) 9, 84
Sau3AI GATC 2 cut(s) 230, 238
SfaNI GCATC 2 cut(s) 279, 351
SfcI CTRYAG 1 cut(s) 268
Sfr303I CCGCGG 1 cut(s) 87
SgrBI CCGCGG 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 3 cut(s) 167, 472, 485
SmoI CTYRAG 3 cut(s) 167, 472, 485
SnaBI TACGTA 1 cut(s) 112
SsiI CCGC 3 cut(s) 8, 84, 86
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 2 cut(s) 108, 114
TaqI TCGA 1 cut(s) 375
TaqII GACCGA 1 cut(s) 206
TauI GCSGC 2 cut(s) 11, 86
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 1 cut(s) 291
TseFI GTSAC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 3 cut(s) 210, 294, 339
TspGWI ACGGA 3 cut(s) 116, 213, 473
TspRI CASTG 1 cut(s) 291
Tth111I GACNNNGTC 1 cut(s) 155
Vha464I CTTAAG 1 cut(s) 167
XmiI GTMKAC 2 cut(s) 108, 375
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.