Rw4G004340

Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013313
Physical Location & Seq
Reverse (-)
8632040 .. 8635341
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004340.1

Sequence Viewer

Length: 489 bp
ATGGCAGAGAACAAAGCAAGCATAACCCATCAGCTCCAGAGTGTGAAACGCAAGTGTGGCAAGTCATATAGTCAGATAGCCAAGGAGACAGGCCTGACCAATGTCTATGTTGCCCAGCTTCTGAGACGCCAAGCTCAGCTCAAGGCTGAAACTGCCCCCAAGTTGAGGGCCGCCCTTCCCGACCTGCCCGAGGACCTGATTGCGGAAATGATGACTCCACCCTTGAGGTCCTATGATCCCCAGTTGATTCAGGATCCCACTATTTACAGGTTGAATGAAGCAGTTATGCATTTTGGTGAAAGCATCAAGGAGATTATCAATGAGGAATATGGTGATGGCATTATGTCAGCTATAGATTTCTACTGCTCAGTCGACAAAGTTAAAGGTGTGGATGGTAAGGACCGTGTAGTTCTGACATTTGATGGGAAATATTTGCCTCATACTGAGCAGAAATCGGAGCACATGGTTTCAAGGCTGAATTTAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008824 GO:0016829 GO:0016840
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Cyanate_lyase

Protein Analysis

162

Amino Acids

18.24

Weight (kDa)

6.43

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyanate_lyase PF02560 81 - 148 7.2e-30 Cyanate lyase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23490 AT3G23490 AT3G23490
fragaria_vesca FvH4_4g04220 FvH4_4g04220
malus_domestica MD13G1218700.v1.1
prunus_persica Prupe.1G045500_v2.0.a1
pyrus_communis pycom13g19370
rosa_chinensis RchiOBHm_Chr4g0394701
rosa_laevigata RLG00000009671
rosa_multiflora Rmu_sc0005356.1_g000020 Rmu_sc0005424.1_g000001
rosa_roxburghii Rroxscaffold_5G00339710
rosa_samantha Rh4AG054400 Rh4BG052100 Rh4CG059000 Rh4DG050700
rosa_wichuraiana Rw4G004340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 372
AciI CCGC 2 cut(s) 171, 203
AclWI GGATC 3 cut(s) 230, 248, 261
AcsI RAATTY 1 cut(s) 478
AcyI GRCGYC 1 cut(s) 127
AfiI CCNNNNNNNGG 3 cut(s) 165, 190, 202
AgsI TTSAA 2 cut(s) 274, 471
AluBI AGCT 5 cut(s) 34, 118, 134, 139, 350
AluI AGCT 5 cut(s) 34, 118, 134, 139, 350
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 2 cut(s) 80, 118
AlwI GGATC 3 cut(s) 230, 248, 261
AlwNI CAGNNNCTG 1 cut(s) 121
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 91, 168
ApoI RAATTY 1 cut(s) 478
AspS9I GGNCC 4 cut(s) 168, 193, 228, 400
AsuHPI GGTGA 2 cut(s) 308, 344
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 3 cut(s) 193, 228, 400
BamHI GGATCC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 462
BccI CCATC 4 cut(s) 36, 329, 386, 416
BcoDI GTCTC 2 cut(s) 80, 118
BfmI CTRYAG 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 192
BisI GCNGC 1 cut(s) 171
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 172
Bme18I GGWCC 3 cut(s) 193, 228, 400
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 4 cut(s) 168, 193, 228, 400
BmiI GGNNCC 1 cut(s) 255
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 235
BoxI GACNNNNGTC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 20
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 125, 244
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 81, 189
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 190, 202
Bse1I ACTGG 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 81, 189
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 3 cut(s) 165, 190, 202
BseMII CTCAG 4 cut(s) 113, 149, 381, 435
BseNI ACTGG 1 cut(s) 241
BseYI CCCAGC 1 cut(s) 114
BshFI GGCC 2 cut(s) 93, 170
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 188
BslI CCNNNNNNNGG 3 cut(s) 165, 190, 202
BsmAI GTCTC 2 cut(s) 80, 118
BsmBI CGTCTC 1 cut(s) 118
BsnI GGCC 2 cut(s) 93, 170
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 462
Bsp143I GATC 2 cut(s) 235, 253
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 2 cut(s) 171, 203
BspANI GGCC 2 cut(s) 93, 170
BspCNI CTCAG 4 cut(s) 114, 148, 380, 436
BspLI GGNNCC 1 cut(s) 255
BspMI ACCTGC 1 cut(s) 192
BspPI GGATC 3 cut(s) 230, 248, 261
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 81, 189
BssMI GATC 2 cut(s) 235, 253
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 404
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 4 cut(s) 122, 135, 367, 444
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 397
BstKTI GATC 2 cut(s) 238, 256
BstMAI GTCTC 2 cut(s) 80, 118
BstMBI GATC 2 cut(s) 235, 253
BstMWI GCNNNNNNNGC 2 cut(s) 57, 152
BstPAI GACNNNNGTC 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 2 cut(s) 93, 170
BtsCI GGATG 1 cut(s) 397
BveI ACCTGC 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 121
Cfr13I GGNCC 4 cut(s) 168, 193, 228, 400
CseI GACGC 1 cut(s) 135
CviAII CATG 1 cut(s) 463
DdeI CTNAG 4 cut(s) 122, 135, 367, 444
DpnI GATC 2 cut(s) 237, 255
DpnII GATC 2 cut(s) 235, 253
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 93
Eco47I GGWCC 3 cut(s) 193, 228, 400
Eco88I CYCGRG 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO109I RGGNCCY 2 cut(s) 193, 228
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 466
FatI CATG 1 cut(s) 462
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 404
Fsp4HI GCNGC 1 cut(s) 171
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 118
GsuI CTGGAG 1 cut(s) 20
HaeIII GGCC 2 cut(s) 93, 170
HgaI GACGC 1 cut(s) 135
Hin1I GRCGYC 1 cut(s) 127
Hin1II CATG 1 cut(s) 466
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 2 cut(s) 214, 247
HphI GGTGA 2 cut(s) 308, 344
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 4 cut(s) 75, 123, 414, 457
Hpy188III TCNNGA 3 cut(s) 37, 179, 251
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 152
HpyF3I CTNAG 4 cut(s) 122, 135, 367, 444
Hsp92I GRCGYC 1 cut(s) 127
Hsp92II CATG 1 cut(s) 466
Kzo9I GATC 2 cut(s) 235, 253
LmnI GCTCC 2 cut(s) 39, 457
LpnPI CCDG 9 cut(s) 50, 75, 107, 128, 197, 209, 236, 253, 254
LweI GCATC 1 cut(s) 312
MalI GATC 2 cut(s) 237, 255
MboI GATC 2 cut(s) 235, 253
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 1 cut(s) 478
MlyI GAGTC 1 cut(s) 208
MnlI CCTC 5 cut(s) 159, 184, 219, 316, 447
Mph1103I ATGCAT 1 cut(s) 291
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 57, 152
NdeII GATC 2 cut(s) 235, 253
NlaIII CATG 1 cut(s) 466
NlaIV GGNNCC 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 291
PceI AGGCCT 1 cut(s) 93
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PpuMI RGGWCCY 2 cut(s) 193, 228
PshAI GACNNNNGTC 1 cut(s) 101
Psp5II RGGWCCY 2 cut(s) 193, 228
PspFI CCCAGC 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 4 cut(s) 168, 193, 228, 400
PspPPI RGGWCCY 2 cut(s) 193, 228
PstNI CAGNNNCTG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 294
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 2 cut(s) 235, 253
Sau96I GGNCC 4 cut(s) 168, 193, 228, 400
SchI GAGTC 1 cut(s) 208
SduI GDGCHC 1 cut(s) 462
SfaNI GCATC 1 cut(s) 312
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 3 cut(s) 193, 228, 400
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 2 cut(s) 140, 223
SmoI CTYRAG 2 cut(s) 140, 223
Sse9I AATT 1 cut(s) 478
SseBI AGGCCT 1 cut(s) 93
SsiI CCGC 2 cut(s) 171, 203
SspI AATATT 1 cut(s) 431
StuI AGGCCT 1 cut(s) 93
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 404
TaqI TCGA 1 cut(s) 372
TasI AATT 1 cut(s) 478
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 291
VpaK11BI GGWCC 3 cut(s) 193, 228, 400
XagI CCTNNNNNAGG 1 cut(s) 188
XapI RAATTY 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 372
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.