Rh4AG054400

Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013313
Physical Location & Seq
Reverse (-)
11307893 .. 11310993
3101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG054400.1

Sequence Viewer

Length: 345 bp
ATGGCAGAGAACAAAGCAAGCATAACCCATCAGCTCCAGAGTGTGAAACGCAGGTGTGGCAAGTCATATAGTCAGATAGCCAAGGAGACAGGCCTGACCAATGTCTATGTTGCCCAGCTTCTGAGACGCCAAGCTCAGCTCAAGGCTGAAACTGCCCCCAAGTTGAGGGCCGCCCTTCCCGACCTGCCCGAGGACCTGATAGCGGAAATGATGACTCCACCCTTGAGGTCATATGATCCCCAGTTGATTCAGGATCCCACTATTTACAGGTTGAATGAAGCAGTTATGCATTTTGGTGAAAGCATCAAGGAGATTATCAATGAGGAATATGGTGATGGCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008824 GO:0016829 GO:0016840
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Cyanate_lyase

Protein Analysis

114

Amino Acids

12.89

Weight (kDa)

6.11

Isoelectric Point (pI)

60.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyanate_lyase PF02560 81 - 113 5.3e-12 Cyanate lyase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23490 AT3G23490 AT3G23490
fragaria_vesca FvH4_4g04220 FvH4_4g04220
malus_domestica MD13G1218700.v1.1
prunus_persica Prupe.1G045500_v2.0.a1
pyrus_communis pycom13g19370
rosa_chinensis RchiOBHm_Chr4g0394701
rosa_laevigata RLG00000009671
rosa_multiflora Rmu_sc0005356.1_g000020 Rmu_sc0005424.1_g000001
rosa_roxburghii Rroxscaffold_5G00339710
rosa_samantha Rh4AG054400 Rh4BG052100 Rh4CG059000 Rh4DG050700
rosa_wichuraiana Rw4G004340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 42
Acc36I ACCTGC 2 cut(s) 42, 192
AciI CCGC 2 cut(s) 171, 203
AclWI GGATC 3 cut(s) 230, 248, 261
AcyI GRCGYC 1 cut(s) 127
AfiI CCNNNNNNNGG 3 cut(s) 165, 190, 202
AgsI TTSAA 1 cut(s) 274
AluBI AGCT 4 cut(s) 34, 118, 134, 139
AluI AGCT 4 cut(s) 34, 118, 134, 139
Alw26I GTCTC 2 cut(s) 80, 118
AlwI GGATC 3 cut(s) 230, 248, 261
AlwNI CAGNNNCTG 1 cut(s) 121
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 91, 168
AspS9I GGNCC 2 cut(s) 168, 193
AsuHPI GGTGA 2 cut(s) 308, 344
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 193
BamHI GGATCC 1 cut(s) 253
BccI CCATC 2 cut(s) 36, 329
BcoDI GTCTC 2 cut(s) 80, 118
BfuAI ACCTGC 2 cut(s) 42, 192
BisI GCNGC 1 cut(s) 171
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 172
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 168, 193
BmiI GGNNCC 1 cut(s) 255
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 235
BoxI GACNNNNGTC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 20
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 125, 244
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 81, 189
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 190, 202
Bse1I ACTGG 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 81, 189
BseLI CCNNNNNNNGG 3 cut(s) 165, 190, 202
BseMII CTCAG 2 cut(s) 113, 149
BseNI ACTGG 1 cut(s) 241
BseYI CCCAGC 1 cut(s) 114
BshFI GGCC 2 cut(s) 93, 170
BsiHKCI CYCGRG 1 cut(s) 188
BslI CCNNNNNNNGG 3 cut(s) 165, 190, 202
BsmAI GTCTC 2 cut(s) 80, 118
BsmBI CGTCTC 1 cut(s) 118
BsnI GGCC 2 cut(s) 93, 170
BsoBI CYCGRG 1 cut(s) 188
Bsp143I GATC 2 cut(s) 235, 253
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 2 cut(s) 171, 203
BspANI GGCC 2 cut(s) 93, 170
BspCNI CTCAG 2 cut(s) 114, 148
BspLI GGNNCC 1 cut(s) 255
BspMI ACCTGC 2 cut(s) 42, 192
BspPI GGATC 3 cut(s) 230, 248, 261
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 81, 189
BssMI GATC 2 cut(s) 235, 253
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 81
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 122, 135
BstENI CCTNNNNNAGG 1 cut(s) 188
BstKTI GATC 2 cut(s) 238, 256
BstMAI GTCTC 2 cut(s) 80, 118
BstMBI GATC 2 cut(s) 235, 253
BstMWI GCNNNNNNNGC 2 cut(s) 57, 152
BstNSI RCATGY 1 cut(s) 343
BstPAI GACNNNNGTC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 2 cut(s) 93, 170
BveI ACCTGC 2 cut(s) 42, 192
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 121
Cfr13I GGNCC 2 cut(s) 168, 193
CseI GACGC 1 cut(s) 135
CviAII CATG 1 cut(s) 340
CviJI RGCY 8 cut(s) 34, 80, 93, 118, 134, 139, 146, 170
CviKI_1 RGCY 8 cut(s) 34, 80, 93, 118, 134, 139, 146, 170
DdeI CTNAG 2 cut(s) 122, 135
DpnI GATC 2 cut(s) 237, 255
DpnII GATC 2 cut(s) 235, 253
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO109I RGGNCCY 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 343
FaiI YATR 9 cut(s) 23, 67, 69, 108, 232, 234, 287, 330, 341
FatI CATG 1 cut(s) 339
FauNDI CATATG 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 118
GsuI CTGGAG 1 cut(s) 20
HaeIII GGCC 2 cut(s) 93, 170
HgaI GACGC 1 cut(s) 135
Hin1I GRCGYC 1 cut(s) 127
Hin1II CATG 1 cut(s) 343
HinfI GANTC 2 cut(s) 214, 247
HphI GGTGA 2 cut(s) 308, 344
Hpy188I TCNGA 2 cut(s) 75, 123
Hpy188III TCNNGA 3 cut(s) 37, 179, 251
HpyAV CCTTC 1 cut(s) 185
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 152
HpyF3I CTNAG 2 cut(s) 122, 135
Hsp92I GRCGYC 1 cut(s) 127
Hsp92II CATG 1 cut(s) 343
Kzo9I GATC 2 cut(s) 235, 253
LmnI GCTCC 1 cut(s) 39
LweI GCATC 1 cut(s) 312
MalI GATC 2 cut(s) 237, 255
MboI GATC 2 cut(s) 235, 253
MflI RGATCY 1 cut(s) 253
MlyI GAGTC 1 cut(s) 208
MnlI CCTC 4 cut(s) 159, 184, 219, 316
Mph1103I ATGCAT 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 57, 152
NdeI CATATG 1 cut(s) 232
NdeII GATC 2 cut(s) 235, 253
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 1 cut(s) 343
PaqCI CACCTGC 1 cut(s) 42
PceI AGGCCT 1 cut(s) 93
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PpuMI RGGWCCY 1 cut(s) 193
PshAI GACNNNNGTC 1 cut(s) 101
Psp5II RGGWCCY 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 168, 193
PspPPI RGGWCCY 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 294
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 2 cut(s) 235, 253
Sau96I GGNCC 2 cut(s) 168, 193
SchI GAGTC 1 cut(s) 208
SetI ASST 9 cut(s) 36, 56, 120, 136, 141, 186, 198, 230, 272
SfaNI GCATC 1 cut(s) 312
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 2 cut(s) 140, 223
SmoI CTYRAG 2 cut(s) 140, 223
SseBI AGGCCT 1 cut(s) 93
SsiI CCGC 2 cut(s) 171, 203
StuI AGGCCT 1 cut(s) 93
StyI CCWWGG 1 cut(s) 81
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 247
TspDTI ATGAA 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 193
XagI CCTNNNNNAGG 1 cut(s) 188
XceI RCATGY 1 cut(s) 343
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.