FvH4_4g04220

Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013313
Physical Location & Seq
Reverse (-)
3635232 .. 3638418
3187 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04220.t1

Sequence Viewer

Length: 489 bp
ATGGCAGAGAACAAAGCAAGCATAACCCTTCAACTCCAGAGTGTGAAACGCAAGTCTGGCAAGTCTTACAGTCAGATAGCCAAGGAGACAGGCCTGACCAATGTCTATGTTGCCCAGCTTCTGAAACGCCAAGCCCAGCTCAAAGCTGAAACTGCCCCCAAGTTGAGGGCTGCCCTTCCCGACCTTCCCGAGGACCTGATTGTGGAGATGATGAGTCCTCCCTTGAGGTCTTATGATCCCCAGTTGATTCAGGACCCCACTATTTACAGGTTGAATGAAGCAGTTATGCATTTTGGTGAAAGCATCAAGGAGATTATCAATGAAGAATATGGTGATGGCATTATGTCAGCTATAGATTTTTACTGCTCAGTCGACAAAGTTAAAGGTGTGGATGGTAAGGATCGTGTAGTTCTGACTTTTGATGGGAAATATTTGCCTCATACTGAGCAGAAGTCGGAGCACATGGTTTCAAGGCTGAATTTAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008824 GO:0016829 GO:0016840
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Cyanate_lyase

Protein Analysis

163

Amino Acids

18.19

Weight (kDa)

6.31

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyanate_lyase PF02560 81 - 148 7.2e-30 Cyanate lyase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23490 AT3G23490 AT3G23490
fragaria_vesca FvH4_4g04220 FvH4_4g04220
malus_domestica MD13G1218700.v1.1
prunus_persica Prupe.1G045500_v2.0.a1
pyrus_communis pycom13g19370
rosa_chinensis RchiOBHm_Chr4g0394701
rosa_laevigata RLG00000009671
rosa_multiflora Rmu_sc0005356.1_g000020 Rmu_sc0005424.1_g000001
rosa_roxburghii Rroxscaffold_5G00339710
rosa_samantha Rh4AG054400 Rh4BG052100 Rh4CG059000 Rh4DG050700
rosa_wichuraiana Rw4G004340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 372
AclWI GGATC 2 cut(s) 230, 408
AcsI RAATTY 1 cut(s) 478
AfiI CCNNNNNNNGG 3 cut(s) 165, 190, 202
AgsI TTSAA 3 cut(s) 32, 274, 471
AluBI AGCT 4 cut(s) 118, 139, 146, 350
AluI AGCT 4 cut(s) 118, 139, 146, 350
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 2 cut(s) 230, 408
AlwNI CAGNNNCTG 1 cut(s) 121
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 478
AspS9I GGNCC 2 cut(s) 193, 253
AsuHPI GGTGA 2 cut(s) 308, 344
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 193, 253
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 1 cut(s) 157
BccI CCATC 3 cut(s) 329, 386, 416
BcoDI GTCTC 1 cut(s) 80
BfmI CTRYAG 1 cut(s) 351
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme18I GGWCC 2 cut(s) 193, 253
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 193, 253
BmiI GGNNCC 1 cut(s) 255
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 235
BoxI GACNNNNGTC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 81, 189
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 190, 202
Bse1I ACTGG 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 81, 189
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 3 cut(s) 165, 190, 202
BseMII CTCAG 2 cut(s) 381, 435
BseNI ACTGG 1 cut(s) 241
BseXI GCAGC 1 cut(s) 157
BseYI CCCAGC 2 cut(s) 114, 135
BshFI GGCC 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 188
BslI CCNNNNNNNGG 3 cut(s) 165, 190, 202
BsmAI GTCTC 1 cut(s) 80
BsnI GGCC 1 cut(s) 93
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 462
Bsp143I GATC 2 cut(s) 235, 400
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 2 cut(s) 380, 436
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 2 cut(s) 230, 408
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 81, 189
BssMI GATC 2 cut(s) 235, 400
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 367, 444
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 397
BstKTI GATC 2 cut(s) 238, 403
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 2 cut(s) 235, 400
BstMWI GCNNNNNNNGC 2 cut(s) 57, 152
BstPAI GACNNNNGTC 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 157
BsuRI GGCC 1 cut(s) 93
BtsCI GGATG 1 cut(s) 397
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 121
Cfr13I GGNCC 2 cut(s) 193, 253
CviAII CATG 1 cut(s) 463
CviJI RGCY 9 cut(s) 80, 93, 118, 134, 139, 146, 170, 350, 475
CviKI_1 RGCY 9 cut(s) 80, 93, 118, 134, 139, 146, 170, 350, 475
DdeI CTNAG 2 cut(s) 367, 444
DpnI GATC 2 cut(s) 237, 402
DpnII GATC 2 cut(s) 235, 400
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 193, 253
Eco88I CYCGRG 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO109I RGGNCCY 2 cut(s) 193, 253
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 1 cut(s) 466
FaiI YATR 9 cut(s) 23, 108, 234, 287, 330, 344, 353, 441, 464
FatI CATG 1 cut(s) 462
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 404
Fsp4HI GCNGC 1 cut(s) 171
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 2 cut(s) 118, 139
GsuI CTGGAG 1 cut(s) 20
HaeIII GGCC 1 cut(s) 93
Hin1II CATG 1 cut(s) 466
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 2 cut(s) 214, 247
HphI GGTGA 2 cut(s) 308, 344
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 4 cut(s) 75, 123, 414, 457
Hpy188III TCNNGA 4 cut(s) 37, 179, 188, 251
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 3 cut(s) 38, 185, 194
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 152
HpyF3I CTNAG 2 cut(s) 367, 444
Hsp92II CATG 1 cut(s) 466
Kzo9I GATC 2 cut(s) 235, 400
LmnI GCTCC 1 cut(s) 457
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 1 cut(s) 312
MalI GATC 2 cut(s) 237, 402
MboI GATC 2 cut(s) 235, 400
MboII GAAGA 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 1 cut(s) 478
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 5 cut(s) 159, 184, 219, 228, 447
Mph1103I ATGCAT 1 cut(s) 291
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 57, 152
NdeII GATC 2 cut(s) 235, 400
NlaIII CATG 1 cut(s) 466
NlaIV GGNNCC 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 291
PceI AGGCCT 1 cut(s) 93
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PpuMI RGGWCCY 2 cut(s) 193, 253
PshAI GACNNNNGTC 1 cut(s) 101
Psp5II RGGWCCY 2 cut(s) 193, 253
PspFI CCCAGC 2 cut(s) 114, 135
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 193, 253
PspPPI RGGWCCY 2 cut(s) 193, 253
PstNI CAGNNNCTG 1 cut(s) 121
RseI CAYNNNNRTG 1 cut(s) 294
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 2 cut(s) 235, 400
Sau96I GGNCC 2 cut(s) 193, 253
SchI GAGTC 1 cut(s) 223
SduI GDGCHC 1 cut(s) 462
SetI ASST 9 cut(s) 120, 141, 148, 186, 198, 230, 272, 352, 388
SfaNI GCATC 1 cut(s) 312
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 2 cut(s) 193, 253
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 1 cut(s) 478
SseBI AGGCCT 1 cut(s) 93
SspI AATATT 1 cut(s) 431
StuI AGGCCT 1 cut(s) 93
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 71
TaqI TCGA 1 cut(s) 372
TasI AATT 1 cut(s) 478
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 2 cut(s) 291, 336
VpaK11BI GGWCC 2 cut(s) 193, 253
XagI CCTNNNNNAGG 1 cut(s) 188
XapI RAATTY 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 372
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.