Prupe.1G045500_v2.0.a1

Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013313
Physical Location & Seq
Reverse (-)
3242813 .. 3245685
2873 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G045500.1

Sequence Viewer

Length: 489 bp
ATGGCAGAGAACAAAGCAATCATAACCCATCAGCTTCAGTCTCTGAAGCGCGAGTCTGGCAAGTCCTATAGTCAGCTAGCGAAGGAGATAGGCCTCACCAACGTGTATGTTGCTCAGCTTCTGAGGCGCCAAGCTCAGCTCAAGCCTGAAACTGCCCCTAAGTTGCAGGCTGCCCTTCCCGGCCTGCCCCAAGAGCTCATTAAGGAGATGATGATTCCACCCTTGAGGTCCTATGACCCTAATTTGATCCAAGATCCCACTATTTACAGGTTGAATGAAGCAGTTATGCATTTTGGTGAAAGCATCAAGGAGATTATCAACGAGGAATTTGGTGATGGCATTATGTCAGCTATAGATTTCTACTGTTCAGTCGACAAAGTTAAAGGTGTGGATGGCAAGGACCGGGTAGCCCTAATATTTGATGGGAAATATTTGCCTCATTCTGAGCAGAAGTCAGAGCACATGGTTTCAAGGCTGCATTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008824 GO:0016829 GO:0016840
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Cyanate_lyase

Protein Analysis

163

Amino Acids

18.33

Weight (kDa)

6.22

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23490 AT3G23490 AT3G23490
fragaria_vesca FvH4_4g04220 FvH4_4g04220
malus_domestica MD13G1218700.v1.1
prunus_persica Prupe.1G045500_v2.0.a1
pyrus_communis pycom13g19370
rosa_chinensis RchiOBHm_Chr4g0394701
rosa_laevigata RLG00000009671
rosa_multiflora Rmu_sc0005356.1_g000020 Rmu_sc0005424.1_g000001
rosa_roxburghii Rroxscaffold_5G00339710
rosa_samantha Rh4AG054400 Rh4BG052100 Rh4CG059000 Rh4DG050700
rosa_wichuraiana Rw4G004340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 372
AccII CGCG 1 cut(s) 51
AclWI GGATC 2 cut(s) 241, 248
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 2 cut(s) 20, 65
AcyI GRCGYC 1 cut(s) 127
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 2 cut(s) 274, 471
AleI CACNNNNGTG 1 cut(s) 101
AluBI AGCT 7 cut(s) 34, 76, 118, 134, 139, 196, 350
AluI AGCT 7 cut(s) 34, 76, 118, 134, 139, 196, 350
Alw21I GWGCWC 2 cut(s) 198, 462
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 2 cut(s) 241, 248
AlwNI CAGNNNCTG 2 cut(s) 43, 121
AoxI GGCC 2 cut(s) 91, 181
ApeKI GCWGC 2 cut(s) 170, 475
ApoI RAATTY 1 cut(s) 326
AspLEI GCGC 2 cut(s) 51, 129
AspS9I GGNCC 2 cut(s) 228, 400
AsuC2I CCSGG 2 cut(s) 180, 404
AsuHPI GGTGA 3 cut(s) 88, 308, 344
AsuNHI GCTAGC 1 cut(s) 76
AvaII GGWCC 2 cut(s) 228, 400
BanI GGYRCC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 198, 462
BbvI GCAGC 2 cut(s) 157, 462
BccI CCATC 4 cut(s) 36, 329, 386, 416
BcnI CCSGG 2 cut(s) 180, 404
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 1 cut(s) 77
BfmI CTRYAG 2 cut(s) 67, 351
BfoI RGCGCY 1 cut(s) 130
BisI GCNGC 2 cut(s) 171, 476
BlpI GCTNAGC 2 cut(s) 114, 135
BlsI GCNGC 2 cut(s) 172, 477
Bme1390I CCNGG 2 cut(s) 180, 404
Bme18I GGWCC 2 cut(s) 228, 400
BmgT120I GGNCC 2 cut(s) 228, 400
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 2 cut(s) 180, 404
BmsI GCATC 1 cut(s) 312
BmtI GCTAGC 1 cut(s) 80
Bpu1102I GCTNAGC 2 cut(s) 114, 135
BpuEI CTTGAG 2 cut(s) 125, 244
BpuMI CCSGG 2 cut(s) 180, 404
BsaHI GRCGYC 1 cut(s) 127
BseGI GGATG 1 cut(s) 397
BseMII CTCAG 4 cut(s) 113, 128, 149, 435
BseXI GCAGC 2 cut(s) 157, 462
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 2 cut(s) 93, 183
BshNI GGYRCC 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 198, 462
BsiSI CCGG 2 cut(s) 180, 403
BsmAI GTCTC 1 cut(s) 45
BsnI GGCC 2 cut(s) 93, 183
Bsp1286I GDGCHC 2 cut(s) 198, 462
Bsp143I GATC 2 cut(s) 246, 253
Bsp1720I GCTNAGC 2 cut(s) 114, 135
BspANI GGCC 2 cut(s) 93, 183
BspCNI CTCAG 4 cut(s) 114, 127, 148, 436
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 128
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 2 cut(s) 241, 248
BspT107I GGYRCC 1 cut(s) 126
BssMI GATC 2 cut(s) 246, 253
BssNI GRCGYC 1 cut(s) 127
Bst4CI ACNGT 1 cut(s) 365
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 3 cut(s) 78, 168, 185
BstDEI CTNAG 5 cut(s) 114, 122, 135, 159, 444
BstF5I GGATG 1 cut(s) 397
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 2 cut(s) 51, 129
BstKTI GATC 2 cut(s) 249, 256
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 2 cut(s) 246, 253
BstMWI GCNNNNNNNGC 3 cut(s) 57, 124, 193
BstSCI CCNGG 2 cut(s) 178, 402
BstSFI CTRYAG 2 cut(s) 67, 351
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 157, 462
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 2 cut(s) 93, 183
BtsCI GGATG 1 cut(s) 397
Cac8I GCNNGC 3 cut(s) 78, 168, 185
CaiI CAGNNNCTG 2 cut(s) 43, 121
CfoI GCGC 2 cut(s) 51, 129
Cfr13I GGNCC 2 cut(s) 228, 400
CviAII CATG 1 cut(s) 463
DdeI CTNAG 5 cut(s) 114, 122, 135, 159, 444
DinI GGCGCC 1 cut(s) 128
DpnI GATC 2 cut(s) 248, 255
DpnII GATC 2 cut(s) 246, 253
Ecl136II GAGCTC 1 cut(s) 196
Eco147I AGGCCT 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 198
Eco47I GGWCC 2 cut(s) 228, 400
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 2 cut(s) 20, 65
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 228
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 198
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
FaeI CATG 1 cut(s) 466
FaiI YATR 8 cut(s) 23, 69, 108, 234, 287, 344, 353, 464
FatI CATG 1 cut(s) 462
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 2 cut(s) 171, 476
FokI GGATG 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 2 cut(s) 171, 476
FspBI CTAG 1 cut(s) 77
GlaI GCGC 2 cut(s) 50, 128
GluI GCNGC 2 cut(s) 171, 476
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 2 cut(s) 93, 183
HapII CCGG 2 cut(s) 180, 403
HhaI GCGC 2 cut(s) 51, 129
Hin1I GRCGYC 1 cut(s) 127
Hin1II CATG 1 cut(s) 466
Hin6I GCGC 2 cut(s) 49, 127
HinP1I GCGC 2 cut(s) 49, 127
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 2 cut(s) 53, 214
HpaII CCGG 2 cut(s) 180, 403
HphI GGTGA 3 cut(s) 88, 308, 344
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 4 cut(s) 45, 123, 445, 457
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 2 cut(s) 76, 185
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 3 cut(s) 166, 289, 478
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 124, 193
HpyF3I CTNAG 5 cut(s) 114, 122, 135, 159, 444
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 127
Hsp92II CATG 1 cut(s) 466
HspAI GCGC 2 cut(s) 49, 127
KasI GGCGCC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 246, 253
LpnPI CCDG 7 cut(s) 42, 152, 159, 193, 197, 253, 416
Lsp1109I GCAGC 2 cut(s) 157, 462
LweI GCATC 1 cut(s) 312
MaeI CTAG 1 cut(s) 77
MaeII ACGT 1 cut(s) 102
MalI GATC 2 cut(s) 248, 255
MboI GATC 2 cut(s) 246, 253
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 2 cut(s) 198, 462
MluCI AATT 2 cut(s) 241, 326
Mly113I GGCGCC 1 cut(s) 127
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 5 cut(s) 104, 117, 219, 316, 447
Mph1103I ATGCAT 1 cut(s) 291
MseI TTAA 2 cut(s) 201, 381
MslI CAYNNNNRTG 2 cut(s) 101, 294
MspI CCGG 2 cut(s) 180, 403
MspR9I CCNGG 2 cut(s) 180, 404
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 3 cut(s) 57, 124, 193
NarI GGCGCC 1 cut(s) 127
NciI CCSGG 2 cut(s) 180, 404
NdeII GATC 2 cut(s) 246, 253
NheI GCTAGC 1 cut(s) 76
NlaIII CATG 1 cut(s) 466
NlaIV GGNNCC 1 cut(s) 128
NsiI ATGCAT 1 cut(s) 291
OliI CACNNNNGTG 1 cut(s) 101
PceI AGGCCT 1 cut(s) 93
PfeI GAWTC 1 cut(s) 214
PkrI GCNGC 2 cut(s) 172, 477
PleI GAGTC 1 cut(s) 61
PluTI GGCGCC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 61
PpuMI RGGWCCY 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 2 cut(s) 228, 400
PspPPI RGGWCCY 1 cut(s) 228
PstNI CAGNNNCTG 2 cut(s) 43, 121
PsuI RGATCY 1 cut(s) 253
RseI CAYNNNNRTG 2 cut(s) 101, 294
SacI GAGCTC 1 cut(s) 198
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 2 cut(s) 201, 381
SatI GCNGC 2 cut(s) 171, 476
Sau3AI GATC 2 cut(s) 246, 253
Sau96I GGNCC 2 cut(s) 228, 400
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 180, 404
SduI GDGCHC 2 cut(s) 198, 462
SfaNI GCATC 1 cut(s) 312
SfcI CTRYAG 2 cut(s) 67, 351
SfoI GGCGCC 1 cut(s) 128
SinI GGWCC 2 cut(s) 228, 400
SmiMI CAYNNNNRTG 2 cut(s) 101, 294
SmlI CTYRAG 2 cut(s) 140, 223
SmoI CTYRAG 2 cut(s) 140, 223
Sse9I AATT 2 cut(s) 241, 326
SseBI AGGCCT 1 cut(s) 93
SspDI GGCGCC 1 cut(s) 126
SspI AATATT 2 cut(s) 417, 431
SspMI CTAG 1 cut(s) 77
SstI GAGCTC 1 cut(s) 198
StuI AGGCCT 1 cut(s) 93
StyD4I CCNGG 2 cut(s) 178, 402
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 372
TasI AATT 2 cut(s) 241, 326
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 2 cut(s) 201, 381
Tru9I TTAA 2 cut(s) 201, 381
TseI GCWGC 2 cut(s) 170, 475
TspDTI ATGAA 1 cut(s) 291
VpaK11BI GGWCC 2 cut(s) 228, 400
XapI RAATTY 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 1 cut(s) 77
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.