AT3G25660

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010317
Physical Location & Seq
Reverse (-)
9339342 .. 9342223
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25660.1

Sequence Viewer

Length: 1614 bp
ATGTTATCGACATTGCAACCGCCGCGTTCTCTATCACTCCTCCCTCTACGCCGGTTCCAAATATCTAAAACCATCGTCTCCGCAGCTTCTTCGAAGACCATCGACACTTCCGTTATCTCTCCACCACAATCTCAAATTCTCACCACTCGTCGTTCACTCCTCTCCGGCGAAACCACAGCTGTCGAAATCGCAAAATCTTACCTTTCTCGTATCCGTCTCACTGAACCTCAGCTCAAATGCTTCCTTCACGTATCGGAGAATGTTCTCAAAGATGCTCAAGAGATTGATCAACGAATCGCTAAAGGTGAGGAATTGGGTCCTCTCGCCGGAGTTTTGATCGGCGTTAAGGATAATATATGTACTCAAGGTATGCCTTCTACTGCTGCTTCGAGAATCTTAGAGCATTATCGTCCACCGTTCGATGCGACGGCGGTTAAGAAGATTAAAGAGTTGGGTGGGATTGTTGTTGGTAAGACTAATATGGATGAGTTTGGAATGGGAAGTACCACTGAAGCTTCTGCTTTTCAGGTAACTGCGAATCCGTGGGATTTGAGTCGTGTACCGGGAGGTTCATCGGGAGGATCAGCAGCAGCTGTTGCAGCAAGGCAGTGTATGGTGTCACTTGGTAGTGATACTGGTGGAAGTGTGAGACAGCCAGCTTCGTTTTGTGGTGTTGTAGGTCTTAAGCCGACTTATGGGCGTGTTTCTAGGTTTGGGCTTATGGCTTATGCGTCTTCGTTGGATGTGATTGGGTGTTTTGGGTCTACGGTTGCTGATGCTGGGATGCTTCTTCATGCTATTTCGGGGTATGATAGGTTTGACTCCACGAGTAGCAAACAAGATGTGCCTGAGTTCCAGTCTCAGTTTCTATCTGTGGATCATTTTGAGTCTAAACCATTGAATGGAGTGAAAGTTGGTATAATTCGCGAGACACTTGAAGACGGTGTTGATTCTGGAGTGAGATCTGCTACTCAGGAAGCTGCGTCTCACTTAGAAGCATTGGGTTGTATATTGACAGAGGTCTCCCTTCCTTCATTCTCTCTTGGACTACCAGCTTACTACGTTATTGCCTCATCGGAGTCATCTTCGAATTTATCACGTTATGATGGTGTCAGATATGGTAATCAAGTTATGGCTGAAGAACTCAATAAGCTGTATGAATGTTCCCGTGGAGAAGGATTTGGAGGAGAGGTGAAAATGAGAATTTTAATGGGGACATATGCACTTTCTGCGGGTTACTACGATGCTTACTACAAGCGAGCTCAACAGGTGAGGACACTAATTCGAAAAGACTTCAAAGCAGCGTTAGAACAGAACGATATCCTTATTTCTCCGGCAGCTCCATCTGCTGCATACAAGATTGGTGAAAAGAAAGATGATCCACTGGCAATGTATGCTGGAGACATTATGACGGTCAATGTGAATCTTGCCGGACTTCCTGCAATGGTATTACCTTGTGGATTAGTCGAAGGTGGTCCCTCGGGTCTTCCTGTTGGTCTTCAAATGATCGGAGCAGCGTTTGATGAGGAGAAATTGCTGAAAGTGGGTCACATATTTGAGCAGACTCTTAAAGGATCGAGCTTTGTTCCTCCATTGTTAGCCAATGTAGCCTAA

Protein Analysis

537

Amino Acids

57.18

Weight (kDa)

6.16

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 62 - 513 1.1e-147 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 902
AccI GTMKAC 1 cut(s) 764
AccII CGCG 2 cut(s) 25, 927
AciI CCGC 5 cut(s) 20, 23, 81, 431, 1232
AclWI GGATC 4 cut(s) 589, 885, 1373, 1582
AcsI RAATTY 3 cut(s) 135, 1090, 1203
AcuI CTGAAG 2 cut(s) 531, 1158
AfaI GTAC 3 cut(s) 361, 505, 561
AfiI CCNNNNNNNGG 3 cut(s) 326, 695, 902
AflII CTTAAG 1 cut(s) 683
AgsI TTSAA 4 cut(s) 901, 938, 1297, 1502
Alw21I GWGCWC 1 cut(s) 1264
Alw26I GTCTC 8 cut(s) 82, 221, 643, 864, 923, 990, 1027, 1395
AlwI GGATC 4 cut(s) 589, 885, 1373, 1582
AlwNI CAGNNNCTG 1 cut(s) 593
Ama87I CYCGRG 1 cut(s) 1480
ApoI RAATTY 3 cut(s) 135, 1090, 1203
AspS9I GGNCC 2 cut(s) 317, 1475
AsuC2I CCSGG 1 cut(s) 564
AsuHPI GGTGA 5 cut(s) 133, 317, 1204, 1282, 1376
AsuII TTCGAA 3 cut(s) 92, 1088, 1285
AvaI CYCGRG 1 cut(s) 1480
AvaII GGWCC 2 cut(s) 317, 1475
BanII GRGCYC 1 cut(s) 1264
BauI CACGAG 1 cut(s) 826
BbsI GAAGAC 5 cut(s) 101, 726, 945, 1478, 1490
Bbv12I GWGCWC 1 cut(s) 1264
BbvCI CCTCAGC 1 cut(s) 228
BccI CCATC 4 cut(s) 80, 107, 1100, 1351
BceAI ACGGC 1 cut(s) 444
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciVI GTATCC 1 cut(s) 221
BclI TGATCA 1 cut(s) 286
BcnI CCSGG 1 cut(s) 564
BcoDI GTCTC 8 cut(s) 82, 221, 643, 864, 923, 990, 1027, 1395
BfaI CTAG 1 cut(s) 708
BfrI CTTAAG 1 cut(s) 683
BfuI GTATCC 1 cut(s) 221
BglII AGATCT 1 cut(s) 962
Bme1390I CCNGG 1 cut(s) 564
Bme18I GGWCC 2 cut(s) 317, 1475
BmeT110I CYCGRG 1 cut(s) 1480
BmgT120I GGNCC 2 cut(s) 317, 1475
BmiI GGNNCC 3 cut(s) 56, 318, 1477
BmrFI CCNGG 1 cut(s) 564
BmsI GCATC 5 cut(s) 262, 412, 766, 774, 1234
BoxI GACNNNNGTC 1 cut(s) 1019
BpiI GAAGAC 5 cut(s) 101, 726, 945, 1478, 1490
BpmI CTGGAG 2 cut(s) 975, 1419
Bpu10I CCTNAGC 1 cut(s) 228
Bpu14I TTCGAA 3 cut(s) 92, 1088, 1285
BpuEI CTTGAG 2 cut(s) 261, 348
BpuMI CCSGG 1 cut(s) 564
BsaAI YACGTR 1 cut(s) 250
BsaI GGTCTC 1 cut(s) 1027
BsaJI CCNNGG 3 cut(s) 542, 1168, 1479
BsaXI ACNNNNNCTCC 4 cut(s) 897, 927, 1176, 1206
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 695, 902
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 3 cut(s) 640, 856, 1389
Bse3DI GCAATG 3 cut(s) 11, 1395, 1449
BseDI CCNNGG 3 cut(s) 542, 1168, 1479
BseGI GGATG 3 cut(s) 490, 748, 789
BseLI CCNNNNNNNGG 3 cut(s) 326, 695, 902
BseMI GCAATG 3 cut(s) 11, 1395, 1449
BseMII CTCAG 4 cut(s) 242, 840, 875, 986
BseNI ACTGG 3 cut(s) 640, 856, 1389
BseRI GAGGAG 4 cut(s) 29, 149, 1200, 1541
BseYI CCCAGC 1 cut(s) 779
Bsh1236I CGCG 2 cut(s) 25, 927
BsiHKAI GWGCWC 1 cut(s) 1264
BsiHKCI CYCGRG 1 cut(s) 1480
BsiSI CCGG 6 cut(s) 52, 165, 327, 563, 1334, 1431
BslFI GGGAC 2 cut(s) 1228, 1461
BslI CCNNNNNNNGG 3 cut(s) 326, 695, 902
BsmAI GTCTC 8 cut(s) 82, 221, 643, 864, 923, 990, 1027, 1395
BsmBI CGTCTC 3 cut(s) 82, 221, 990
BsmFI GGGAC 2 cut(s) 1228, 1461
Bso31I GGTCTC 1 cut(s) 1027
BsoBI CYCGRG 1 cut(s) 1480
Bsp119I TTCGAA 3 cut(s) 92, 1088, 1285
Bsp1286I GDGCHC 1 cut(s) 1264
Bsp143I GATC 8 cut(s) 286, 336, 581, 877, 962, 1378, 1506, 1574
Bsp68I TCGCGA 1 cut(s) 927
BspACI CCGC 5 cut(s) 20, 23, 81, 431, 1232
BspCNI CTCAG 4 cut(s) 241, 841, 874, 985
BspFNI CGCG 2 cut(s) 25, 927
BspLI GGNNCC 3 cut(s) 56, 318, 1477
BspPI GGATC 4 cut(s) 589, 885, 1373, 1582
BspT104I TTCGAA 3 cut(s) 92, 1088, 1285
BspTI CTTAAG 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 1027
BsrDI GCAATG 3 cut(s) 11, 1395, 1449
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 3 cut(s) 640, 856, 1389
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 542, 1168, 1479
BssMI GATC 8 cut(s) 286, 336, 581, 877, 962, 1378, 1506, 1574
BssSI CACGAG 1 cut(s) 826
Bst2BI CACGAG 1 cut(s) 826
Bst4CI ACNGT 4 cut(s) 417, 769, 944, 1414
BstAFI CTTAAG 1 cut(s) 683
BstAPI GCANNNNNTGC 3 cut(s) 596, 1229, 1394
BstBAI YACGTR 1 cut(s) 250
BstBI TTCGAA 3 cut(s) 92, 1088, 1285
BstC8I GCNNGC 2 cut(s) 657, 1260
BstDEI CTNAG 6 cut(s) 228, 397, 849, 861, 972, 991
BstDSI CCRYGG 2 cut(s) 542, 1168
BstF5I GGATG 3 cut(s) 490, 748, 789
BstFNI CGCG 2 cut(s) 25, 927
BstKTI GATC 8 cut(s) 289, 339, 584, 880, 965, 1381, 1509, 1577
BstMAI GTCTC 8 cut(s) 82, 221, 643, 864, 923, 990, 1027, 1395
BstMBI GATC 8 cut(s) 286, 336, 581, 877, 962, 1378, 1506, 1574
BstMWI GCNNNNNNNGC 7 cut(s) 22, 596, 599, 1229, 1346, 1394, 1607
BstPAI GACNNNNGTC 1 cut(s) 1019
BstSCI CCNGG 1 cut(s) 562
BstUI CGCG 2 cut(s) 25, 927
BstV2I GAAGAC 5 cut(s) 101, 726, 945, 1478, 1490
BstX2I RGATCY 1 cut(s) 962
BstYI RGATCY 1 cut(s) 962
BsuI GTATCC 1 cut(s) 221
BtgI CCRYGG 2 cut(s) 542, 1168
BtsCI GGATG 3 cut(s) 490, 748, 789
BtsI GCAGTG 1 cut(s) 614
BtsIMutI CAGTG 4 cut(s) 219, 507, 614, 1382
BtuMI TCGCGA 1 cut(s) 927
Cac8I GCNNGC 2 cut(s) 657, 1260
CaiI CAGNNNCTG 1 cut(s) 593
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 2 cut(s) 317, 1475
CseI GACGC 2 cut(s) 720, 972
Csp6I GTAC 3 cut(s) 360, 504, 560
CviAII CATG 1 cut(s) 794
CviQI GTAC 3 cut(s) 360, 504, 560
DdeI CTNAG 6 cut(s) 228, 397, 849, 861, 972, 991
DpnI GATC 8 cut(s) 288, 338, 583, 879, 964, 1380, 1508, 1576
DpnII GATC 8 cut(s) 286, 336, 581, 877, 962, 1378, 1506, 1574
Ecl136II GAGCTC 1 cut(s) 1262
Eco24I GRGCYC 1 cut(s) 1264
Eco31I GGTCTC 1 cut(s) 1027
Eco32I GATATC 1 cut(s) 1321
Eco47I GGWCC 2 cut(s) 317, 1475
Eco53kI GAGCTC 1 cut(s) 1262
Eco57I CTGAAG 2 cut(s) 531, 1158
Eco88I CYCGRG 1 cut(s) 1480
EcoICRI GAGCTC 1 cut(s) 1262
EcoO109I RGGNCCY 1 cut(s) 317
EcoRV GATATC 1 cut(s) 1321
EcoT38I GRGCYC 1 cut(s) 1264
Esp3I CGTCTC 3 cut(s) 82, 221, 990
FaeI CATG 1 cut(s) 797
FaqI GGGAC 2 cut(s) 1228, 1461
FatI CATG 1 cut(s) 793
FauI CCCGC 1 cut(s) 1225
FauNDI CATATG 1 cut(s) 1219
FbaI TGATCA 1 cut(s) 286
FblI GTMKAC 1 cut(s) 764
FokI GGATG 3 cut(s) 497, 755, 796
FriOI GRGCYC 1 cut(s) 1264
FspBI CTAG 1 cut(s) 708
GsaI CCCAGC 1 cut(s) 783
GsuI CTGGAG 2 cut(s) 975, 1419
HapII CCGG 6 cut(s) 52, 165, 327, 563, 1334, 1431
HgaI GACGC 2 cut(s) 720, 972
Hin1II CATG 1 cut(s) 797
HindIII AAGCTT 1 cut(s) 513
HpaII CCGG 6 cut(s) 52, 165, 327, 563, 1334, 1431
HphI GGTGA 5 cut(s) 133, 317, 1204, 1282, 1376
Hpy166II GTNNAC 4 cut(s) 155, 413, 560, 765
Hpy188I TCNGA 4 cut(s) 256, 1078, 1115, 1511
Hpy188III TCNNGA 6 cut(s) 278, 390, 576, 926, 954, 974
Hpy8I GTNNAC 4 cut(s) 155, 413, 560, 765
Hpy99I CGWCG 2 cut(s) 153, 430
HpyAV CCTTC 6 cut(s) 254, 384, 1037, 1041, 1169, 1463
HpyCH4III ACNGT 4 cut(s) 417, 769, 944, 1414
HpyCH4IV ACGT 3 cut(s) 249, 1062, 1099
HpyCH4V TGCA 5 cut(s) 16, 599, 1223, 1352, 1442
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 596, 599, 1229, 1346, 1394, 1607
HpyF3I CTNAG 6 cut(s) 228, 397, 849, 861, 972, 991
HpySE526I ACGT 3 cut(s) 249, 1062, 1099
Hsp92II CATG 1 cut(s) 797
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 8 cut(s) 286, 336, 581, 877, 962, 1378, 1506, 1574
LmnI GCTCC 2 cut(s) 1345, 1511
LweI GCATC 5 cut(s) 262, 412, 766, 774, 1234
MaeI CTAG 1 cut(s) 708
MaeII ACGT 3 cut(s) 249, 1062, 1099
MaeIII GTNAC 4 cut(s) 529, 618, 1235, 1547
MalI GATC 8 cut(s) 288, 338, 583, 879, 964, 1380, 1508, 1576
MboI GATC 8 cut(s) 286, 336, 581, 877, 962, 1378, 1506, 1574
MflI RGATCY 1 cut(s) 962
MhlI GDGCHC 1 cut(s) 1264
MluCI AATT 7 cut(s) 135, 311, 921, 1090, 1203, 1281, 1532
MlyI GAGTC 5 cut(s) 562, 815, 896, 1088, 1558
MmeI TCCRAC 1 cut(s) 720
MseI TTAA 6 cut(s) 345, 435, 444, 684, 1208, 1569
MspA1I CMGCKG 2 cut(s) 179, 593
MspCI CTTAAG 1 cut(s) 683
MspI CCGG 6 cut(s) 52, 165, 327, 563, 1334, 1431
MspR9I CCNGG 1 cut(s) 564
MvnI CGCG 2 cut(s) 25, 927
MwoI GCNNNNNNNGC 7 cut(s) 22, 596, 599, 1229, 1346, 1394, 1607
NciI CCSGG 1 cut(s) 564
NdeI CATATG 1 cut(s) 1219
NdeII GATC 8 cut(s) 286, 336, 581, 877, 962, 1378, 1506, 1574
NlaIII CATG 1 cut(s) 797
NlaIV GGNNCC 3 cut(s) 56, 318, 1477
NmuCI GTSAC 2 cut(s) 618, 1547
NruI TCGCGA 1 cut(s) 927
NspV TTCGAA 3 cut(s) 92, 1088, 1285
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 5 cut(s) 294, 393, 538, 950, 1423
PflMI CCANNNNNTGG 1 cut(s) 902
PleI GAGTC 5 cut(s) 561, 815, 895, 1087, 1558
PpsI GAGTC 5 cut(s) 561, 815, 895, 1087, 1558
Ppu21I YACGTR 1 cut(s) 250
PpuMI RGGWCCY 1 cut(s) 317
PshAI GACNNNNGTC 1 cut(s) 1019
Psp124BI GAGCTC 1 cut(s) 1264
Psp5II RGGWCCY 1 cut(s) 317
PspFI CCCAGC 1 cut(s) 779
PspN4I GGNNCC 3 cut(s) 56, 318, 1477
PspPI GGNCC 2 cut(s) 317, 1475
PspPPI RGGWCCY 1 cut(s) 317
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 962
PvuII CAGCTG 2 cut(s) 179, 593
RruI TCGCGA 1 cut(s) 927
RsaI GTAC 3 cut(s) 361, 505, 561
RsaNI GTAC 3 cut(s) 360, 504, 560
SacI GAGCTC 1 cut(s) 1264
SaqAI TTAA 6 cut(s) 345, 435, 444, 684, 1208, 1569
Sau3AI GATC 8 cut(s) 286, 336, 581, 877, 962, 1378, 1506, 1574
Sau96I GGNCC 2 cut(s) 317, 1475
SchI GAGTC 5 cut(s) 562, 815, 896, 1088, 1558
ScrFI CCNGG 1 cut(s) 564
SduI GDGCHC 1 cut(s) 1264
SfaNI GCATC 5 cut(s) 262, 412, 766, 774, 1234
SfuI TTCGAA 3 cut(s) 92, 1088, 1285
SinI GGWCC 2 cut(s) 317, 1475
SmlI CTYRAG 3 cut(s) 276, 363, 683
SmoI CTYRAG 3 cut(s) 276, 363, 683
Sse9I AATT 7 cut(s) 135, 311, 921, 1090, 1203, 1281, 1532
SsiI CCGC 5 cut(s) 20, 23, 81, 431, 1232
SspMI CTAG 1 cut(s) 708
SstI GAGCTC 1 cut(s) 1264
StyD4I CCNGG 1 cut(s) 562
TaaI ACNGT 4 cut(s) 417, 769, 944, 1414
TaiI ACGT 3 cut(s) 252, 1065, 1102
TasI AATT 7 cut(s) 135, 311, 921, 1090, 1203, 1281, 1532
TatI WGTACW 1 cut(s) 359
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 5 cut(s) 294, 393, 538, 950, 1423
Tru1I TTAA 6 cut(s) 345, 435, 444, 684, 1208, 1569
Tru9I TTAA 6 cut(s) 345, 435, 444, 684, 1208, 1569
TscAI CASTG 4 cut(s) 226, 514, 614, 1389
TseFI GTSAC 2 cut(s) 618, 1547
Tsp45I GTSAC 2 cut(s) 618, 1547
TspDTI ATGAA 4 cut(s) 561, 782, 1023, 1173
TspGWI ACGGA 3 cut(s) 100, 203, 531
TspRI CASTG 4 cut(s) 226, 514, 614, 1389
Van91I CCANNNNNTGG 1 cut(s) 902
Vha464I CTTAAG 1 cut(s) 683
VpaK11BI GGWCC 2 cut(s) 317, 1475
XapI RAATTY 3 cut(s) 135, 1090, 1203
XmiI GTMKAC 1 cut(s) 764
XspI CTAG 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.