Rw2G039500

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010317
Physical Location & Seq
Forward (+)
64837033 .. 64841549
4517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039500.1

Sequence Viewer

Length: 1638 bp
ATGCTATCCACACTCCAACCTCCGCGCTCACTCTCTCACTTCCGCACTAAACCCCTCCTCCTCCGCCGCCACACCTCCCTTAAACCCTTCTCCTCCTTATCCTCACAGCCCGTCACCCACTCCACCCTCACAGACCAATCCAAATCCCAAATCCTCTCCACCCGAGACTCTCTCCTCTCCCGCCAAATCACCGCCGCCGAGCTCGCCGAGTCCTTCCTCGCCCGCCTCCGCCGCACCGAGCCCCACCTCCGCTCCTTCCTCCACGTCTCCGACGCCGTCCTCGCCCAGGCCCGCGACCTCGACGACAAGCTTCGCCTGAATGAGGAGGTGGGACCCTTGGCCGGGGTCCTTGTGGCGGTGAAGGATAACATATGTACGGCGGAGATGCCGTCCACGGCGGGGTCCAGGGTTTTGGAGGGCTATACGCCGCCGTATGATGCCACCGCCGTCAGGAAGATCAAGGAGTTGGGTGGGATTGTGGTGGGCAAGACCAATTTGGATGAGTTTGGGATGGGTAGCACCACCGAAGGCTCCGCCTTTCAGGTGACTGCAAACCCATGGGACACGACACGGGTGCCAGGAGGGTCATCAGGAGGCTCGGCTGCAGCTGTATCTGCTAGGCAGAGTATGGTATCATTGGGAAGTGATACTGGTGGAAGTGTGAGGCAGCCAGCATCGTTTTGTGGTGTTGTTGGGTTGAAGCCCACGTATGGGCGTGTATCGAGGTTTGGACTTATGGCATATGCGTCATCTCTTGATGTTATTGGCTGCTTTGGAACATCTGTTGCTGATGCTGGGATTCTTCTTCATGCAATTTCTGGTCATGATAGACTTGATGCTACCAGCAGCAAGCGTGAGGTTCCTGACCTTGCATCTCAATTTGTTTCCATTAGTTTGCTACAGTCTAAACCTCTGAAAGGACTAAAAATTGGTCTGATCCGTGAAACTCTCGATGATGGTGTTGATATTGGAGTAACTTCTGCAATTCGTACTGCTGCACTGCATCTTGAAGAATTAGGATGCTCCGTAACAGAGGTCTCATTACCATCTTTCTCACTTGGATTGCCAGCCTACTATGTCATTGCTTCATCCGAGTCATCTTCGAACTTATCGCGTTATGATGGTCTCAGGTATGGAAACCAAGTTGCTGCTGATGAGCTGAACTCGCAATACATGGACTCCCGCGCCAAGGGATTTGGTTCTGAGGTCAAAAGGAGAATTCTGACCGGAACATATGCCCTTTCAGCTGGTTATTATGATGCATATTATAAACGCGCCCAGCAGGTGAGGACGATAATTCGGAAAAGCTTCAAGGCGGCACTGGACGAACATGACATCTTAATCTCACCTGCTGCTCCATCTGCTGCGTATAAGATTGGTGAAAAGAAAAATGATCCATTAGCAATGTATGCAGGCGACATAATGACGGTTAATGTTAACTTGGCAGGTCTACCTGCATTGGTTCTGCCATGTGGATTTGCCGAAGGGGGGTCAGCAGGCCTTCCGGTTGGTCTTCAAATGATTGGTGCAGCATTCGATGAGGAAAAGTTGCTTAGAGTGGGCCACATCTTTGAGCAGACCCTTCAAAATTGCAGATTTGTACCTCCATTGATAGCAGATGAGGATATACCATGCTAG

Protein Analysis

545

Amino Acids

58.15

Weight (kDa)

7.14

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 67 - 518 3.5e-143 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1269
AarI CACCTGC 2 cut(s) 1273, 1357
Acc36I ACCTGC 4 cut(s) 1273, 1357, 1436, 1462
AccB1I GGYRCC 1 cut(s) 574
AccBSI CCGCTC 1 cut(s) 252
AccI GTMKAC 1 cut(s) 1450
AccII CGCG 5 cut(s) 25, 294, 1114, 1185, 1275
AclWI GGATC 2 cut(s) 931, 1388
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 1 cut(s) 1218
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 3 cut(s) 376, 991, 1602
AgsI TTSAA 5 cut(s) 700, 1010, 1312, 1517, 1586
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 3 cut(s) 285, 404, 577
AluBI AGCT 6 cut(s) 202, 310, 608, 1159, 1247, 1308
AluI AGCT 6 cut(s) 202, 310, 608, 1159, 1247, 1308
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 4 cut(s) 159, 271, 1042, 1130
AlwI GGATC 2 cut(s) 931, 1388
Ama87I CYCGRG 1 cut(s) 162
AoxI GGCC 4 cut(s) 288, 339, 1498, 1561
ApoI RAATTY 1 cut(s) 1218
Asp700I GAANNNNTTC 1 cut(s) 1307
AspLEI GCGC 3 cut(s) 27, 1187, 1277
AspS9I GGNCC 5 cut(s) 289, 332, 346, 402, 1561
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 7 cut(s) 106, 181, 370, 556, 1297, 1338, 1391
AsuII TTCGAA 1 cut(s) 1103
AvaI CYCGRG 1 cut(s) 162
AvaII GGWCC 3 cut(s) 332, 346, 402
BanI GGYRCC 1 cut(s) 574
BanII GRGCYC 2 cut(s) 204, 243
BbsI GAAGAC 1 cut(s) 1505
Bbv12I GWGCWC 1 cut(s) 204
BccI CCATC 5 cut(s) 505, 950, 1054, 1115, 1366
BceAI ACGGC 6 cut(s) 260, 373, 393, 411, 415, 431
BcgI CGANNNNNNTGC 2 cut(s) 556, 590
BciT130I CCWGG 3 cut(s) 287, 406, 579
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 4 cut(s) 159, 271, 1042, 1130
BfaI CTAG 2 cut(s) 618, 1636
BfmI CTRYAG 2 cut(s) 603, 899
BfuAI ACCTGC 4 cut(s) 1273, 1357, 1436, 1462
Bme1390I CCNGG 4 cut(s) 287, 343, 406, 579
Bme18I GGWCC 3 cut(s) 332, 346, 402
BmeT110I CYCGRG 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 5 cut(s) 289, 332, 346, 402, 1561
BmiI GGNNCC 7 cut(s) 333, 334, 347, 403, 532, 576, 861
BmrFI CCNGG 4 cut(s) 287, 343, 406, 579
BmsI GCATC 9 cut(s) 375, 427, 683, 781, 826, 881, 1010, 1012, 1249
BpiI GAAGAC 1 cut(s) 1505
BplI GAGNNNNNCTC 4 cut(s) 156, 188, 1148, 1180
Bpu14I TTCGAA 1 cut(s) 1103
BpuMI CCSGG 1 cut(s) 343
BsaAI YACGTR 1 cut(s) 708
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 2 cut(s) 1042, 1130
BsaJI CCNNGG 7 cut(s) 285, 336, 342, 393, 405, 557, 1188
BsaWI WCCGGW 2 cut(s) 1226, 1504
BsaXI ACNNNNNCTCC 8 cut(s) 42, 72, 236, 266, 374, 404, 1163, 1193
Bse1I ACTGG 2 cut(s) 655, 1326
Bse3DI GCAATG 2 cut(s) 1080, 1410
BseBI CCWGG 3 cut(s) 287, 406, 579
BseDI CCNNGG 7 cut(s) 285, 336, 342, 393, 405, 557, 1188
BseGI GGATG 4 cut(s) 505, 516, 1025, 1088
BseMI GCAATG 2 cut(s) 1080, 1410
BseMII CTCAG 2 cut(s) 1141, 1194
BseNI ACTGG 2 cut(s) 655, 1326
BseRI GAGGAG 5 cut(s) 47, 50, 82, 164, 338
BseYI CCCAGC 2 cut(s) 794, 1278
BsgI GTGCAG 2 cut(s) 981, 1548
Bsh1236I CGCG 5 cut(s) 25, 294, 1114, 1185, 1275
BshFI GGCC 4 cut(s) 290, 341, 1500, 1563
BshNI GGYRCC 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 162
BsiSI CCGG 3 cut(s) 342, 1227, 1505
BslFI GGGAC 2 cut(s) 345, 575
BsmAI GTCTC 4 cut(s) 159, 271, 1042, 1130
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 2 cut(s) 345, 575
BsmI GAATGC 1 cut(s) 1532
BsnI GGCC 4 cut(s) 290, 341, 1500, 1563
Bso31I GGTCTC 2 cut(s) 1042, 1130
BsoBI CYCGRG 1 cut(s) 162
Bsp119I TTCGAA 1 cut(s) 1103
Bsp1286I GDGCHC 2 cut(s) 204, 243
Bsp143I GATC 3 cut(s) 456, 936, 1393
Bsp19I CCATGG 1 cut(s) 557
BspANI GGCC 4 cut(s) 290, 341, 1500, 1563
BspCNI CTCAG 2 cut(s) 1140, 1195
BspFNI CGCG 5 cut(s) 25, 294, 1114, 1185, 1275
BspHI TCATGA 1 cut(s) 823
BspLI GGNNCC 7 cut(s) 333, 334, 347, 403, 532, 576, 861
BspMAI CTGCAG 1 cut(s) 607
BspMI ACCTGC 4 cut(s) 1273, 1357, 1436, 1462
BspPI GGATC 2 cut(s) 931, 1388
BspT104I TTCGAA 1 cut(s) 1103
BspT107I GGYRCC 1 cut(s) 574
BspTNI GGTCTC 2 cut(s) 1042, 1130
BsrBI CCGCTC 1 cut(s) 252
BsrDI GCAATG 2 cut(s) 1080, 1410
BsrI ACTGG 2 cut(s) 655, 1326
BssECI CCNNGG 7 cut(s) 285, 336, 342, 393, 405, 557, 1188
BssMI GATC 3 cut(s) 456, 936, 1393
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 3 cut(s) 336, 557, 1188
Bst2UI CCWGG 3 cut(s) 287, 406, 579
Bst4CI ACNGT 2 cut(s) 903, 1429
BstACI GRCGYC 1 cut(s) 273
BstAPI GCANNNNNTGC 1 cut(s) 1409
BstBAI YACGTR 1 cut(s) 708
BstBI TTCGAA 1 cut(s) 1103
BstC8I GCNNGC 8 cut(s) 204, 223, 292, 672, 851, 1068, 1414, 1498
BstDEI CTNAG 3 cut(s) 1127, 1203, 1553
BstDSI CCRYGG 2 cut(s) 393, 557
BstENI CCTNNNNNAGG 3 cut(s) 284, 320, 915
BstF5I GGATG 4 cut(s) 505, 516, 1025, 1088
BstFNI CGCG 5 cut(s) 25, 294, 1114, 1185, 1275
BstHHI GCGC 3 cut(s) 27, 1187, 1277
BstKTI GATC 3 cut(s) 459, 939, 1396
BstMAI GTCTC 4 cut(s) 159, 271, 1042, 1130
BstMBI GATC 3 cut(s) 456, 936, 1393
BstMWI GCNNNNNNNGC 8 cut(s) 203, 231, 281, 614, 1165, 1244, 1361, 1409
BstNI CCWGG 3 cut(s) 287, 406, 579
BstSCI CCNGG 4 cut(s) 285, 341, 404, 577
BstSFI CTRYAG 2 cut(s) 603, 899
BstUI CGCG 5 cut(s) 25, 294, 1114, 1185, 1275
BstV2I GAAGAC 1 cut(s) 1505
BstXI CCANNNNNNTGG 1 cut(s) 412
BsuRI GGCC 4 cut(s) 290, 341, 1500, 1563
BtgI CCRYGG 2 cut(s) 393, 557
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 4 cut(s) 505, 516, 1025, 1088
BtsI GCAGTG 1 cut(s) 998
BtsIMutI CAGTG 2 cut(s) 998, 1319
BveI ACCTGC 4 cut(s) 1273, 1357, 1436, 1462
Cac8I GCNNGC 8 cut(s) 204, 223, 292, 672, 851, 1068, 1414, 1498
CciI TCATGA 1 cut(s) 823
CfoI GCGC 3 cut(s) 27, 1187, 1277
Cfr13I GGNCC 5 cut(s) 289, 332, 346, 402, 1561
CseI GACGC 2 cut(s) 281, 735
Csp6I GTAC 3 cut(s) 375, 990, 1601
CviAII CATG 7 cut(s) 558, 809, 824, 1174, 1331, 1470, 1632
CviQI GTAC 3 cut(s) 375, 990, 1601
DdeI CTNAG 3 cut(s) 1127, 1203, 1553
DpnI GATC 3 cut(s) 458, 938, 1395
DpnII GATC 3 cut(s) 456, 936, 1393
EaeI YGGCCR 1 cut(s) 339
EciI GGCGGA 4 cut(s) 53, 218, 395, 523
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 3 cut(s) 336, 557, 1188
Eco147I AGGCCT 1 cut(s) 1500
Eco24I GRGCYC 2 cut(s) 204, 243
Eco31I GGTCTC 2 cut(s) 1042, 1130
Eco47I GGWCC 3 cut(s) 332, 346, 402
Eco53kI GAGCTC 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 162
EcoICRI GAGCTC 1 cut(s) 202
EcoNI CCTNNNNNAGG 3 cut(s) 284, 320, 915
EcoO109I RGGNCCY 2 cut(s) 332, 346
EcoRI GAATTC 1 cut(s) 1218
EcoRII CCWGG 3 cut(s) 285, 404, 577
EcoT14I CCWWGG 3 cut(s) 336, 557, 1188
EcoT22I ATGCAT 1 cut(s) 1264
EcoT38I GRGCYC 2 cut(s) 204, 243
ErhI CCWWGG 3 cut(s) 336, 557, 1188
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 7 cut(s) 561, 812, 827, 1177, 1334, 1473, 1635
FaqI GGGAC 2 cut(s) 345, 575
FatI CATG 7 cut(s) 557, 808, 823, 1173, 1330, 1469, 1631
FauI CCCGC 5 cut(s) 188, 230, 299, 391, 1190
FauNDI CATATG 3 cut(s) 371, 742, 1234
FblI GTMKAC 1 cut(s) 1450
FokI GGATG 4 cut(s) 512, 523, 1032, 1075
FriOI GRGCYC 2 cut(s) 204, 243
FspBI CTAG 2 cut(s) 618, 1636
GlaI GCGC 3 cut(s) 26, 1186, 1276
GsaI CCCAGC 2 cut(s) 798, 1282
HaeIII GGCC 4 cut(s) 290, 341, 1500, 1563
HapII CCGG 3 cut(s) 342, 1227, 1505
HgaI GACGC 2 cut(s) 281, 735
HhaI GCGC 3 cut(s) 27, 1187, 1277
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 7 cut(s) 561, 812, 827, 1177, 1334, 1473, 1635
Hin6I GCGC 3 cut(s) 25, 1185, 1275
HinP1I GCGC 3 cut(s) 25, 1185, 1275
HincII GTYRAC 1 cut(s) 1438
HindII GTYRAC 1 cut(s) 1438
HindIII AAGCTT 2 cut(s) 308, 1306
HinfI GANTC 5 cut(s) 167, 209, 799, 1094, 1178
HpaI GTTAAC 1 cut(s) 1438
HpaII CCGG 3 cut(s) 342, 1227, 1505
HphI GGTGA 7 cut(s) 106, 181, 370, 556, 1297, 1338, 1391
Hpy166II GTNNAC 3 cut(s) 393, 1438, 1451
Hpy188I TCNGA 7 cut(s) 271, 915, 936, 1093, 1204, 1224, 1302
Hpy188III TCNNGA 7 cut(s) 451, 591, 755, 824, 863, 950, 1007
Hpy8I GTNNAC 3 cut(s) 393, 1438, 1451
Hpy99I CGWCG 2 cut(s) 275, 305
HpyAV CCTTC 8 cut(s) 97, 223, 265, 355, 521, 1478, 1511, 1592
HpyCH4III ACNGT 2 cut(s) 903, 1429
HpyCH4IV ACGT 2 cut(s) 264, 707
HpyF10VI GCNNNNNNNGC 8 cut(s) 203, 231, 281, 614, 1165, 1244, 1361, 1409
HpyF3I CTNAG 3 cut(s) 1127, 1203, 1553
HpySE526I ACGT 2 cut(s) 264, 707
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 7 cut(s) 561, 812, 827, 1177, 1334, 1473, 1635
HspAI GCGC 3 cut(s) 25, 1185, 1275
KflI GGGWCCC 1 cut(s) 332
KspAI GTTAAC 1 cut(s) 1438
Kzo9I GATC 3 cut(s) 456, 936, 1393
LmnI GCTCC 4 cut(s) 257, 536, 1028, 1360
LweI GCATC 9 cut(s) 375, 427, 683, 781, 826, 881, 1010, 1012, 1249
MaeI CTAG 2 cut(s) 618, 1636
MaeII ACGT 2 cut(s) 264, 707
MaeIII GTNAC 4 cut(s) 112, 544, 973, 1027
MalI GATC 3 cut(s) 458, 938, 1395
MbiI CCGCTC 1 cut(s) 252
MboI GATC 3 cut(s) 456, 936, 1393
MboII GAAGA 6 cut(s) 466, 794, 797, 1022, 1092, 1505
MhlI GDGCHC 2 cut(s) 204, 243
MluCI AATT 9 cut(s) 493, 813, 878, 927, 984, 1013, 1218, 1296, 1588
MlyI GAGTC 4 cut(s) 161, 218, 1103, 1172
MmeI TCCRAC 2 cut(s) 40, 294
Mph1103I ATGCAT 1 cut(s) 1264
MroXI GAANNNNTTC 1 cut(s) 1307
MseI TTAA 4 cut(s) 81, 1340, 1431, 1437
MspA1I CMGCKG 2 cut(s) 608, 1247
MspI CCGG 3 cut(s) 342, 1227, 1505
MspR9I CCNGG 4 cut(s) 287, 343, 406, 579
Mva1269I GAATGC 1 cut(s) 1532
MvaI CCWGG 3 cut(s) 287, 406, 579
MvnI CGCG 5 cut(s) 25, 294, 1114, 1185, 1275
MwoI GCNNNNNNNGC 8 cut(s) 203, 231, 281, 614, 1165, 1244, 1361, 1409
NciI CCSGG 1 cut(s) 343
NcoI CCATGG 1 cut(s) 557
NdeI CATATG 3 cut(s) 371, 742, 1234
NdeII GATC 3 cut(s) 456, 936, 1393
NlaIII CATG 7 cut(s) 561, 812, 827, 1177, 1334, 1473, 1635
NlaIV GGNNCC 7 cut(s) 333, 334, 347, 403, 532, 576, 861
NmeAIII GCCGAG 3 cut(s) 223, 232, 578
NmuCI GTSAC 2 cut(s) 112, 544
NsiI ATGCAT 1 cut(s) 1264
NspV TTCGAA 1 cut(s) 1103
PagI TCATGA 1 cut(s) 823
PaqCI CACCTGC 2 cut(s) 1273, 1357
PceI AGGCCT 1 cut(s) 1500
PctI GAATGC 1 cut(s) 1532
PdmI GAANNNNTTC 1 cut(s) 1307
PfeI GAWTC 1 cut(s) 799
PflFI GACNNNGTC 1 cut(s) 275
PleI GAGTC 4 cut(s) 161, 217, 1102, 1172
PpsI GAGTC 4 cut(s) 161, 217, 1102, 1172
Ppu21I YACGTR 1 cut(s) 708
PpuMI RGGWCCY 2 cut(s) 332, 346
PsiI TTATAA 1 cut(s) 1269
Psp124BI GAGCTC 1 cut(s) 204
Psp5II RGGWCCY 2 cut(s) 332, 346
Psp6I CCWGG 3 cut(s) 285, 404, 577
PspFI CCCAGC 2 cut(s) 794, 1278
PspGI CCWGG 3 cut(s) 285, 404, 577
PspN4I GGNNCC 7 cut(s) 333, 334, 347, 403, 532, 576, 861
PspPI GGNCC 5 cut(s) 289, 332, 346, 402, 1561
PspPPI RGGWCCY 2 cut(s) 332, 346
PsrI GAACNNNNNNTAC 2 cut(s) 1154, 1186
PstI CTGCAG 1 cut(s) 607
PsyI GACNNNGTC 1 cut(s) 275
PvuII CAGCTG 2 cut(s) 608, 1247
RsaI GTAC 3 cut(s) 376, 991, 1602
RsaNI GTAC 3 cut(s) 375, 990, 1601
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 4 cut(s) 81, 1340, 1431, 1437
Sau3AI GATC 3 cut(s) 456, 936, 1393
Sau96I GGNCC 5 cut(s) 289, 332, 346, 402, 1561
SchI GAGTC 4 cut(s) 161, 218, 1103, 1172
ScrFI CCNGG 4 cut(s) 287, 343, 406, 579
SduI GDGCHC 2 cut(s) 204, 243
SfaNI GCATC 9 cut(s) 375, 427, 683, 781, 826, 881, 1010, 1012, 1249
SfcI CTRYAG 2 cut(s) 603, 899
SfuI TTCGAA 1 cut(s) 1103
SinI GGWCC 3 cut(s) 332, 346, 402
Sse9I AATT 9 cut(s) 493, 813, 878, 927, 984, 1013, 1218, 1296, 1588
SseBI AGGCCT 1 cut(s) 1500
SspMI CTAG 2 cut(s) 618, 1636
SstI GAGCTC 1 cut(s) 204
StuI AGGCCT 1 cut(s) 1500
StyD4I CCNGG 4 cut(s) 285, 341, 404, 577
StyI CCWWGG 3 cut(s) 336, 557, 1188
TaaI ACNGT 2 cut(s) 903, 1429
TaiI ACGT 2 cut(s) 267, 710
TaqI TCGA 5 cut(s) 300, 722, 951, 1103, 1536
TasI AATT 9 cut(s) 493, 813, 878, 927, 984, 1013, 1218, 1296, 1588
TauI GCSGC 5 cut(s) 69, 197, 234, 430, 1319
TfiI GAWTC 1 cut(s) 799
Tru1I TTAA 4 cut(s) 81, 1340, 1431, 1437
Tru9I TTAA 4 cut(s) 81, 1340, 1431, 1437
TscAI CASTG 2 cut(s) 1005, 1326
TseFI GTSAC 2 cut(s) 112, 544
Tsp45I GTSAC 2 cut(s) 112, 544
TspDTI ATGAA 2 cut(s) 797, 1077
TspGWI ACGGA 2 cut(s) 929, 1015
TspRI CASTG 2 cut(s) 1005, 1326
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 3 cut(s) 332, 346, 402
XagI CCTNNNNNAGG 3 cut(s) 284, 320, 915
XapI RAATTY 1 cut(s) 1218
XmiI GTMKAC 1 cut(s) 1450
XmnI GAANNNNTTC 1 cut(s) 1307
XspI CTAG 2 cut(s) 618, 1636
Zsp2I ATGCAT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.