pycom09g07540

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010317
Physical Location & Seq
Reverse (-)
5717364 .. 5721102
3739 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g07540.1

Sequence Viewer

Length: 1656 bp
ATGCTATCGACACTCCAACCGCCGCGCTCGCTTTCCCACTTCCGCACAAACCCCACCCTCCTCCTCCCCTACACCTCCCTTAAACCCCTCTCCTCCTTATCCTCACAGCCCGTCACCCACTCCACCCTAACAGACCAACCCCGCTCCCCAAACCTACTTCCCCACTCCCAAATCCTCTCCACCCGCCACTCCCTCCTCTCCCGCCACCTCACCGCCGCCCAACTCGCCGACTCCTTCCTCGCCCGCCTCCGCCTCACCGAGCCCCACCTCCGCTCCTTCCTCCACGTCTCCGACTCGGTCCTCGCTCAAGCCCGCGAGCTCGACGAGAAGATTCAGAGGAATGAGGAGTTGGGTCCCCTGGCTGGGGTCCTTGTCGCCGTCAAGGACAATATCTGCACGGCTGAGATGCCGTCAACCGCTGGGTCTCGTGTTCTGGAGGGCTATACGCCGCCGTACGACGCCACTGCCGTCAGGAAGATTAAAGAGCTGGGTGGGATTGTGGTTGGAAAGACCAATTTGGATGAGTTTGGCATGGGTAGCACCACTGAAGGCTCTGCATTTCAGGTGACTGCGAACCCGTGGGACGTTTCTCGGGTGCCAGGAGGGTCATCGGGAGGCTCCGCTGCAGCTGTGTCTGCTAGGCAGTGTATGGTATCCTTGGGAAGTGATACTGGCGGAAGTGTGAGGCAGCCCGCATCTTTTTGTGGTGTTGTTGGATTGAAGCCCACGTATGGGCGTGTCTCAAGATTCGGACTTATGGCATATGCGTCGTCGCTTGATGTTATTGGCTGCTTTGGTACGTCTGTTGCTGATGCTGGTATTCTTCTTCAGACAATTTCAGGTCATGATAGACTTGATGCAACCAGCAGCAAGCGTGAGGTTCCCGACCTTGCAGCTGAATTTGTTTCCCTAAGTTCACCAAATTCTAAACCTCTTAAAGGATTGAGAATTGGTCTGATCCGGGAAACTCTGGATGGTGGGGTTGATACTGGAGTAACTTCTGCAGTTCGTAATGCTGCTTTGCATCTTGAAGAATTAGGATGTTCCGTAACCGAGGTCTCATTACCATCTTTCTCACTTGGGTTGCCAGCCTATTACATTCTTGCTTCTTCTGAGTCGTCTTCAAACTTATCACGTTATGATGGTGTCAGGTATGGAAACCATGTTGCTGCTGATGAGTTGAACTCACAATATAAGGATTCCCGAGCCAAGGGTTTTGGTTCTGAGGTTAAAAGGAGAATTCTAACAGGAACATATGCCCTTTCAGCTGGTTATTATGATGCATATTATAAGCGCGCCCAGCAGGTGAGGACTATAATTCGGAAAAGCTTCAAGGCAGCACTAGATGAACATGACATCTTAATTTCACCTGCGGCTCCATCTGCTGCTTATAAGATTGGTGAAAAGAAAAACGATCCTTTAGCAATGTATGCAGGTGACATAATGACTGTCAATGTTAACTTAGCAGGTCTACCTGCACTGGTTTTGCCATGTGGATTTGTTGAGGGGGGTTCTGCAGGCCTTCCTGTTGTTCTTCAAATGATCGGTGCAGCATTTGACGAGGGAAAGTTGCTTAAGGTGGGTCATATATTTGAGCAGACTCTGCAGAATTGCAATTTTGTTCCTCCATTGATAGCAGATGAGCTCACATCCTAG

Protein Analysis

552

Amino Acids

58.57

Weight (kDa)

7.19

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 77 - 525 1.4e-142 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1290, 1392
AarI CACCTGC 3 cut(s) 1294, 1378, 1424
Acc36I ACCTGC 5 cut(s) 1294, 1378, 1424, 1457, 1483
AccB1I GGYRCC 1 cut(s) 595
AccBSI CCGCTC 2 cut(s) 144, 273
AccI GTMKAC 1 cut(s) 1471
AccII CGCG 3 cut(s) 25, 315, 1296
AclWI GGATC 2 cut(s) 952, 1409
AcsI RAATTY 3 cut(s) 899, 922, 1239
AcuI CTGAAG 2 cut(s) 567, 812
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 2 cut(s) 455, 799
AfiI CCNNNNNNNGG 7 cut(s) 362, 363, 364, 731, 732, 938, 1210
AflII CTTAAG 1 cut(s) 1574
AgsI TTSAA 6 cut(s) 721, 1031, 1125, 1183, 1333, 1538
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 2 cut(s) 357, 598
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 7 cut(s) 319, 487, 629, 896, 1268, 1329, 1645
AluI AGCT 7 cut(s) 319, 487, 629, 896, 1268, 1329, 1645
Alw21I GWGCWC 2 cut(s) 321, 1647
Alw26I GTCTC 4 cut(s) 292, 429, 745, 1063
AlwI GGATC 2 cut(s) 952, 1409
AlwNI CAGNNNCTG 1 cut(s) 1603
Ama87I CYCGRG 2 cut(s) 591, 1203
AoxI GGCC 1 cut(s) 1519
ApoI RAATTY 3 cut(s) 899, 922, 1239
Asp700I GAANNNNTTC 1 cut(s) 1328
AspLEI GCGC 3 cut(s) 27, 1296, 1298
AspS9I GGNCC 3 cut(s) 298, 353, 367
AsuC2I CCSGG 1 cut(s) 962
AsuHPI GGTGA 9 cut(s) 106, 202, 247, 577, 909, 1318, 1359, 1412, 1448
AvaI CYCGRG 2 cut(s) 591, 1203
AvaII GGWCC 3 cut(s) 298, 353, 367
BaeI ACNNNNGTAYC 4 cut(s) 789, 822, 978, 1011
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 3 cut(s) 264, 321, 1647
BauI CACGAG 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 1113
Bbv12I GWGCWC 2 cut(s) 321, 1647
BccI CCATC 4 cut(s) 968, 1075, 1136, 1387
BceAI ACGGC 5 cut(s) 362, 394, 414, 436, 452
BcgI CGANNNNNNTGC 4 cut(s) 446, 480, 750, 784
BciT130I CCWGG 2 cut(s) 359, 600
BciVI GTATCC 1 cut(s) 664
BcnI CCSGG 1 cut(s) 962
BcoDI GTCTC 4 cut(s) 292, 429, 745, 1063
BfaI CTAG 3 cut(s) 639, 1343, 1654
BfmI CTRYAG 4 cut(s) 624, 1002, 1515, 1604
BfrI CTTAAG 1 cut(s) 1574
BfuAI ACCTGC 5 cut(s) 1294, 1378, 1424, 1457, 1483
BfuI GTATCC 1 cut(s) 664
Bme1390I CCNGG 3 cut(s) 359, 600, 962
Bme18I GGWCC 3 cut(s) 298, 353, 367
BmeT110I CYCGRG 2 cut(s) 591, 1203
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 298, 353, 367
BmiI GGNNCC 7 cut(s) 354, 355, 368, 597, 619, 882, 1377
BmrFI CCNGG 3 cut(s) 359, 600, 962
BmsI GCATC 6 cut(s) 396, 704, 802, 847, 1033, 1270
BpiI GAAGAC 1 cut(s) 1113
BplI GAGNNNNNCTC 2 cut(s) 1169, 1201
BpmI CTGGAG 2 cut(s) 455, 1011
BpuEI CTTGAG 2 cut(s) 291, 727
BpuMI CCSGG 1 cut(s) 962
BsaAI YACGTR 1 cut(s) 729
BsaHI GRCGYC 1 cut(s) 459
BsaI GGTCTC 2 cut(s) 429, 1063
BsaJI CCNNGG 5 cut(s) 357, 578, 657, 1053, 1209
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 7 cut(s) 362, 363, 364, 731, 732, 938, 1210
Bse1I ACTGG 3 cut(s) 676, 994, 1485
Bse3DI GCAATG 1 cut(s) 1431
BseBI CCWGG 2 cut(s) 359, 600
BseDI CCNNGG 5 cut(s) 357, 578, 657, 1053, 1209
BseGI GGATG 4 cut(s) 526, 979, 1046, 1649
BseLI CCNNNNNNNGG 7 cut(s) 362, 363, 364, 731, 732, 938, 1210
BseMI GCAATG 1 cut(s) 1431
BseMII CTCAG 3 cut(s) 393, 1104, 1215
BseNI ACTGG 3 cut(s) 676, 994, 1485
BsePI GCGCGC 1 cut(s) 1294
BseRI GAGGAG 5 cut(s) 50, 53, 82, 185, 359
BseYI CCCAGC 4 cut(s) 362, 419, 487, 1299
BsgI GTGCAG 3 cut(s) 379, 1461, 1569
Bsh1236I CGCG 3 cut(s) 25, 315, 1296
BshFI GGCC 1 cut(s) 1521
BshNI GGYRCC 1 cut(s) 595
BsiHKAI GWGCWC 2 cut(s) 321, 1647
BsiHKCI CYCGRG 2 cut(s) 591, 1203
BsiSI CCGG 1 cut(s) 961
BsiWI CGTACG 1 cut(s) 453
BslFI GGGAC 2 cut(s) 339, 596
BslI CCNNNNNNNGG 7 cut(s) 362, 363, 364, 731, 732, 938, 1210
BsmAI GTCTC 4 cut(s) 292, 429, 745, 1063
BsmBI CGTCTC 1 cut(s) 292
BsmFI GGGAC 2 cut(s) 339, 596
BsnI GGCC 1 cut(s) 1521
Bso31I GGTCTC 2 cut(s) 429, 1063
BsoBI CYCGRG 2 cut(s) 591, 1203
Bsp1286I GDGCHC 3 cut(s) 264, 321, 1647
Bsp143I GATC 3 cut(s) 957, 1414, 1542
BspANI GGCC 1 cut(s) 1521
BspCNI CTCAG 3 cut(s) 394, 1105, 1216
BspFNI CGCG 3 cut(s) 25, 315, 1296
BspHI TCATGA 1 cut(s) 844
BspLI GGNNCC 7 cut(s) 354, 355, 368, 597, 619, 882, 1377
BspMAI CTGCAG 4 cut(s) 628, 1006, 1519, 1608
BspMI ACCTGC 5 cut(s) 1294, 1378, 1424, 1457, 1483
BspPI GGATC 2 cut(s) 952, 1409
BspT107I GGYRCC 1 cut(s) 595
BspTI CTTAAG 1 cut(s) 1574
BspTNI GGTCTC 2 cut(s) 429, 1063
BsrBI CCGCTC 2 cut(s) 144, 273
BsrDI GCAATG 1 cut(s) 1431
BsrI ACTGG 3 cut(s) 676, 994, 1485
BssECI CCNNGG 5 cut(s) 357, 578, 657, 1053, 1209
BssHII GCGCGC 1 cut(s) 1294
BssMI GATC 3 cut(s) 957, 1414, 1542
BssNI GRCGYC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 426
BssT1I CCWWGG 2 cut(s) 657, 1209
Bst2BI CACGAG 1 cut(s) 426
Bst2UI CCWGG 2 cut(s) 359, 600
Bst4CI ACNGT 1 cut(s) 1450
BstACI GRCGYC 1 cut(s) 459
BstAFI CTTAAG 1 cut(s) 1574
BstAPI GCANNNNNTGC 2 cut(s) 1430, 1603
BstBAI YACGTR 1 cut(s) 729
BstC8I GCNNGC 9 cut(s) 29, 244, 313, 317, 693, 872, 1089, 1296, 1519
BstDEI CTNAG 5 cut(s) 402, 911, 1113, 1224, 1462
BstDSI CCRYGG 1 cut(s) 578
BstENI CCTNNNNNAGG 1 cut(s) 936
BstF5I GGATG 4 cut(s) 526, 979, 1046, 1649
BstFNI CGCG 3 cut(s) 25, 315, 1296
BstHHI GCGC 3 cut(s) 27, 1296, 1298
BstKTI GATC 3 cut(s) 960, 1417, 1545
BstMAI GTCTC 4 cut(s) 292, 429, 745, 1063
BstMBI GATC 3 cut(s) 957, 1414, 1542
BstMWI GCNNNNNNNGC 9 cut(s) 28, 224, 537, 635, 1265, 1300, 1382, 1430, 1603
BstNI CCWGG 2 cut(s) 359, 600
BstSCI CCNGG 3 cut(s) 357, 598, 960
BstSFI CTRYAG 4 cut(s) 624, 1002, 1515, 1604
BstUI CGCG 3 cut(s) 25, 315, 1296
BstV2I GAAGAC 1 cut(s) 1113
BsuI GTATCC 1 cut(s) 664
BsuRI GGCC 1 cut(s) 1521
BtgI CCRYGG 1 cut(s) 578
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 4 cut(s) 526, 979, 1046, 1649
BtsI GCAGTG 2 cut(s) 462, 650
BtsIMutI CAGTG 4 cut(s) 462, 543, 650, 1478
BveI ACCTGC 5 cut(s) 1294, 1378, 1424, 1457, 1483
Cac8I GCNNGC 9 cut(s) 29, 244, 313, 317, 693, 872, 1089, 1296, 1519
CaiI CAGNNNCTG 1 cut(s) 1603
CciI TCATGA 1 cut(s) 844
CfoI GCGC 3 cut(s) 27, 1296, 1298
Cfr13I GGNCC 3 cut(s) 298, 353, 367
CseI GACGC 2 cut(s) 467, 756
Csp6I GTAC 2 cut(s) 454, 798
CviAII CATG 5 cut(s) 532, 845, 1163, 1352, 1491
CviQI GTAC 2 cut(s) 454, 798
DdeI CTNAG 5 cut(s) 402, 911, 1113, 1224, 1462
DpnI GATC 3 cut(s) 959, 1416, 1544
DpnII GATC 3 cut(s) 957, 1414, 1542
EciI GGCGGA 2 cut(s) 239, 690
Ecl136II GAGCTC 2 cut(s) 319, 1645
Eco130I CCWWGG 2 cut(s) 657, 1209
Eco147I AGGCCT 1 cut(s) 1521
Eco24I GRGCYC 3 cut(s) 264, 321, 1647
Eco31I GGTCTC 2 cut(s) 429, 1063
Eco47I GGWCC 3 cut(s) 298, 353, 367
Eco53kI GAGCTC 2 cut(s) 319, 1645
Eco57I CTGAAG 2 cut(s) 567, 812
Eco88I CYCGRG 2 cut(s) 591, 1203
EcoICRI GAGCTC 2 cut(s) 319, 1645
EcoNI CCTNNNNNAGG 1 cut(s) 936
EcoO109I RGGNCCY 2 cut(s) 353, 367
EcoRI GAATTC 1 cut(s) 1239
EcoRII CCWGG 2 cut(s) 357, 598
EcoT14I CCWWGG 2 cut(s) 657, 1209
EcoT22I ATGCAT 1 cut(s) 1285
EcoT38I GRGCYC 3 cut(s) 264, 321, 1647
ErhI CCWWGG 2 cut(s) 657, 1209
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 5 cut(s) 535, 848, 1166, 1355, 1494
FaqI GGGAC 2 cut(s) 339, 596
FatI CATG 5 cut(s) 531, 844, 1162, 1351, 1490
FauI CCCGC 6 cut(s) 149, 191, 209, 251, 320, 700
FauNDI CATATG 2 cut(s) 763, 1255
FblI GTMKAC 1 cut(s) 1471
FokI GGATG 4 cut(s) 533, 986, 1053, 1636
FriOI GRGCYC 3 cut(s) 264, 321, 1647
FspBI CTAG 3 cut(s) 639, 1343, 1654
GlaI GCGC 3 cut(s) 26, 1295, 1297
GsaI CCCAGC 4 cut(s) 366, 423, 491, 1303
GsuI CTGGAG 2 cut(s) 455, 1011
HaeIII GGCC 1 cut(s) 1521
HapII CCGG 1 cut(s) 961
HgaI GACGC 2 cut(s) 467, 756
HhaI GCGC 3 cut(s) 27, 1296, 1298
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 5 cut(s) 535, 848, 1166, 1355, 1494
Hin6I GCGC 3 cut(s) 25, 1294, 1296
HinP1I GCGC 3 cut(s) 25, 1294, 1296
HincII GTYRAC 2 cut(s) 414, 1459
HindII GTYRAC 2 cut(s) 414, 1459
HindIII AAGCTT 1 cut(s) 1327
HinfI GANTC 7 cut(s) 230, 293, 331, 747, 1115, 1199, 1600
HpaI GTTAAC 1 cut(s) 1459
HpaII CCGG 1 cut(s) 961
HphI GGTGA 9 cut(s) 106, 202, 247, 577, 909, 1318, 1359, 1412, 1448
Hpy166II GTNNAC 4 cut(s) 414, 917, 1459, 1472
Hpy188I TCNGA 8 cut(s) 292, 336, 752, 831, 957, 1114, 1225, 1323
Hpy188III TCNNGA 9 cut(s) 434, 472, 612, 744, 845, 884, 971, 1028, 1203
Hpy8I GTNNAC 4 cut(s) 414, 917, 1459, 1472
Hpy99I CGWCG 4 cut(s) 326, 461, 772, 775
HpyAV CCTTC 4 cut(s) 244, 286, 542, 1532
HpyCH4III ACNGT 1 cut(s) 1450
HpyCH4IV ACGT 5 cut(s) 285, 585, 728, 800, 1135
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 224, 537, 635, 1265, 1300, 1382, 1430, 1603
HpyF3I CTNAG 5 cut(s) 402, 911, 1113, 1224, 1462
HpySE526I ACGT 5 cut(s) 285, 585, 728, 800, 1135
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 5 cut(s) 535, 848, 1166, 1355, 1494
HspAI GCGC 3 cut(s) 25, 1294, 1296
KflI GGGWCCC 1 cut(s) 353
KspAI GTTAAC 1 cut(s) 1459
Kzo9I GATC 3 cut(s) 957, 1414, 1542
LmnI GCTCC 4 cut(s) 149, 278, 623, 1381
LweI GCATC 6 cut(s) 396, 704, 802, 847, 1033, 1270
MaeI CTAG 3 cut(s) 639, 1343, 1654
MaeII ACGT 5 cut(s) 285, 585, 728, 800, 1135
MaeIII GTNAC 5 cut(s) 112, 565, 994, 1048, 1436
MalI GATC 3 cut(s) 959, 1416, 1544
MbiI CCGCTC 2 cut(s) 144, 273
MboI GATC 3 cut(s) 957, 1414, 1542
MboII GAAGA 8 cut(s) 340, 487, 815, 818, 1043, 1101, 1113, 1526
MhlI GDGCHC 3 cut(s) 264, 321, 1647
MlyI GAGTC 4 cut(s) 224, 287, 1124, 1594
MmeI TCCRAC 4 cut(s) 40, 315, 484, 694
Mph1103I ATGCAT 1 cut(s) 1285
MroXI GAANNNNTTC 1 cut(s) 1328
MseI TTAA 7 cut(s) 81, 480, 936, 1230, 1361, 1458, 1575
MspA1I CMGCKG 5 cut(s) 419, 623, 629, 896, 1268
MspCI CTTAAG 1 cut(s) 1574
MspI CCGG 1 cut(s) 961
MspR9I CCNGG 3 cut(s) 359, 600, 962
MvaI CCWGG 2 cut(s) 359, 600
MvnI CGCG 3 cut(s) 25, 315, 1296
MwoI GCNNNNNNNGC 9 cut(s) 28, 224, 537, 635, 1265, 1300, 1382, 1430, 1603
NciI CCSGG 1 cut(s) 962
NdeI CATATG 2 cut(s) 763, 1255
NdeII GATC 3 cut(s) 957, 1414, 1542
NlaIII CATG 5 cut(s) 535, 848, 1166, 1355, 1494
NlaIV GGNNCC 7 cut(s) 354, 355, 368, 597, 619, 882, 1377
NmuCI GTSAC 3 cut(s) 112, 565, 1436
NsiI ATGCAT 1 cut(s) 1285
PagI TCATGA 1 cut(s) 844
PaqCI CACCTGC 3 cut(s) 1294, 1378, 1424
PauI GCGCGC 1 cut(s) 1294
PceI AGGCCT 1 cut(s) 1521
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 1328
PfeI GAWTC 3 cut(s) 331, 747, 1199
Pfl23II CGTACG 1 cut(s) 453
PflFI GACNNNGTC 1 cut(s) 296
PfoI TCCNGGA 1 cut(s) 960
PleI GAGTC 4 cut(s) 224, 287, 1123, 1594
PpsI GAGTC 4 cut(s) 224, 287, 1123, 1594
Ppu21I YACGTR 1 cut(s) 729
PpuMI RGGWCCY 2 cut(s) 353, 367
PsiI TTATAA 2 cut(s) 1290, 1392
Psp124BI GAGCTC 2 cut(s) 321, 1647
Psp5II RGGWCCY 2 cut(s) 353, 367
Psp6I CCWGG 2 cut(s) 357, 598
PspFI CCCAGC 4 cut(s) 362, 419, 487, 1299
PspGI CCWGG 2 cut(s) 357, 598
PspLI CGTACG 1 cut(s) 453
PspN4I GGNNCC 7 cut(s) 354, 355, 368, 597, 619, 882, 1377
PspPI GGNCC 3 cut(s) 298, 353, 367
PspPPI RGGWCCY 2 cut(s) 353, 367
PstI CTGCAG 4 cut(s) 628, 1006, 1519, 1608
PstNI CAGNNNCTG 1 cut(s) 1603
PsyI GACNNNGTC 1 cut(s) 296
PteI GCGCGC 1 cut(s) 1294
PvuII CAGCTG 3 cut(s) 629, 896, 1268
RsaI GTAC 2 cut(s) 455, 799
RsaNI GTAC 2 cut(s) 454, 798
SacI GAGCTC 2 cut(s) 321, 1647
SaqAI TTAA 7 cut(s) 81, 480, 936, 1230, 1361, 1458, 1575
Sau3AI GATC 3 cut(s) 957, 1414, 1542
Sau96I GGNCC 3 cut(s) 298, 353, 367
SchI GAGTC 4 cut(s) 224, 287, 1124, 1594
ScrFI CCNGG 3 cut(s) 359, 600, 962
SduI GDGCHC 3 cut(s) 264, 321, 1647
SfaNI GCATC 6 cut(s) 396, 704, 802, 847, 1033, 1270
SfcI CTRYAG 4 cut(s) 624, 1002, 1515, 1604
SinI GGWCC 3 cut(s) 298, 353, 367
SmlI CTYRAG 3 cut(s) 306, 742, 1574
SmoI CTYRAG 3 cut(s) 306, 742, 1574
SseBI AGGCCT 1 cut(s) 1521
SspMI CTAG 3 cut(s) 639, 1343, 1654
SstI GAGCTC 2 cut(s) 321, 1647
StuI AGGCCT 1 cut(s) 1521
StyD4I CCNGG 3 cut(s) 357, 598, 960
StyI CCWWGG 2 cut(s) 657, 1209
TaaI ACNGT 1 cut(s) 1450
TaiI ACGT 5 cut(s) 288, 588, 731, 803, 1138
TaqI TCGA 2 cut(s) 8, 321
TaqII GACCGA 1 cut(s) 286
TauI GCSGC 4 cut(s) 25, 218, 451, 1376
TfiI GAWTC 3 cut(s) 331, 747, 1199
Tru1I TTAA 7 cut(s) 81, 480, 936, 1230, 1361, 1458, 1575
Tru9I TTAA 7 cut(s) 81, 480, 936, 1230, 1361, 1458, 1575
TscAI CASTG 4 cut(s) 469, 550, 650, 1485
TseFI GTSAC 3 cut(s) 112, 565, 1436
Tsp45I GTSAC 3 cut(s) 112, 565, 1436
TspDTI ATGAA 1 cut(s) 1362
TspGWI ACGGA 1 cut(s) 1036
TspRI CASTG 4 cut(s) 469, 550, 650, 1485
Tth111I GACNNNGTC 1 cut(s) 296
Vha464I CTTAAG 1 cut(s) 1574
VpaK11BI GGWCC 3 cut(s) 298, 353, 367
XagI CCTNNNNNAGG 1 cut(s) 936
XapI RAATTY 3 cut(s) 899, 922, 1239
XmiI GTMKAC 1 cut(s) 1471
XmnI GAANNNNTTC 1 cut(s) 1328
XspI CTAG 3 cut(s) 639, 1343, 1654
Zsp2I ATGCAT 1 cut(s) 1285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.