AT3G46980

anion transporter 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004615
Physical Location & Seq
Reverse (-)
17306599 .. 17310068
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G46980.2

Sequence Viewer

Length: 1602 bp
ATGTGTTACTCTCTCTCTATACAATCTTCAATTGACTTCCATAATCGAAACGCTCTAAAAATCCATGGAGATCGAGCAATTTTGACTTCGAACTTACCTACACTCAGGAGGATTCCGTTTTTGCCGGAGCGAGATCGCCGGAGAAAGTTGGTCCTCTGTACCGGGAGAGTCGTAAACTCATTGAAATTCACCGGAAACACTTCCGTCGATCTATGTGGTATTCCTCGTCATCGATTAAGAGTTTCTTGCAGCGATGCTCGCCGTACACCGGAAGAAACGGCGGCGGAGTTGACGGCGCAACCCAATTTTTCAGAATTTATAACGTCGGAGAGAGTCAAAGTGGTTGCGATGCTCGCGCTTGCTTTGGCGCTATGCAATGCGGATCGTGTTGTGATGTCCGTTGCGATTGTTCCTCTTTCGCTTTCTCGTGGATGGAGCAAATCATTTTCCGGTATCGTTCAGTCATCGTTTCTATGGGGATACTTAATTTCTCCAATTGCTGGTGGAACTTTGGTGGACCGTTATGGTGGTAAAGTAGTCATGGCGTGGGGTGTGGCTTTGTGGTCGTTGGCAACTTTTCTTACCCCTTGGGCGGCTGATAGTTCTTTGTGGGCTCTTCTTGCTGCAAGAGCGATGGTTGGGGTTGCTGAAGGAGTGGCTCTTCCTTGTATGAACAACATGGTTGCGAGATGGTTTCCCCCAACAGAACGTTCTAGGGCGGTTGGTATTGCAATGGCAGGGTTTCAGCTTGGGAATGTTGTAGGACTCATGTTGTCTCCTATTCTTATGTCTCAAGGAGGTATATATGGCCCATTTGTAATTTTTGGACTATCGGGGTTCCTTTGGTTGCTCGTGTGGCTCTCTGCAACGTCAAGTGCACCAGATCGACATCCTCAGATTACAAAGTCTGAACTTGAGTATATAAAGCAAAAGAAGCAAATATCTACTATGGAGAACAAACGAATTAGCACAAGTGGAATACCTCCTTTTGGACGCCTTCTCTCCAAGATGCCGACTTGGGCTGTTATAGTTGCAAATTCCATGCATAGCTGGGGTTTCTTTGTGATTCTTTCATGGATGCCCATATACTTCAATTCAGTATATCATGTGAACCTCAAACAAGCTGCTTGGTTTAGTGCCGTTCCATGGAGTATGATGGCTTTCACAGGATACATTGCTGGTTTTTGGTCGGATTTGTTGATACGGCGTGGTACAAGCATCACCTTAACGCGAAAAATCATGCAGTCCATTGGTTTCATTGGTCCTGGAATTGCTCTCATTGGTCTAACGACGGCAAAACAACCTTTAGTGGCATCAGCCTGGCTTAGTTTAGCCGTTGGGCTTAAATCATTCAGCCATTTGGGGTTTCTTATTAATCTTCAGGAAATTGCTCCCGAATATTCCGGTGTTCTACATGGAATGTGTCTTACTGCTGGGACATTAGCTGCGATAGTTGGCACAGTTGGAGCTGGTTTCTTTGTAGAACTATTGGGCTCCTTCCAGGGATTCATCCTTCTTACAGCAATATTGTATCTCCTTTCTGCTCTCTTTTACAACATATACGCAACTGGAGAAAGAGTAGATTTTGACACAACAGCTTGA

Protein Analysis

533

Amino Acids

58.24

Weight (kDa)

9.8

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 1 - 306 4.5e-35 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 320
AccB7I CCANNNNNTGG 1 cut(s) 500
AccII CGCG 2 cut(s) 356, 1231
AciI CCGC 5 cut(s) 281, 284, 380, 593, 719
AclI AACGTT 1 cut(s) 709
AclWI GGATC 1 cut(s) 390
AcsI RAATTY 3 cut(s) 185, 314, 1036
AcuI CTGAAG 2 cut(s) 669, 1364
AcyI GRCGYC 1 cut(s) 994
AfaI GTAC 3 cut(s) 160, 265, 1213
AfiI CCNNNNNNNGG 5 cut(s) 268, 500, 592, 989, 1146
AgsI TTSAA 3 cut(s) 30, 184, 1093
AjnI CCWGG 3 cut(s) 1264, 1319, 1500
AjuI GAANNNNNNNTTGG 2 cut(s) 694, 726
AluBI AGCT 6 cut(s) 748, 1050, 1124, 1445, 1469, 1598
AluI AGCT 6 cut(s) 748, 1050, 1124, 1445, 1469, 1598
Alw21I GWGCWC 1 cut(s) 880
Alw26I GTCTC 2 cut(s) 780, 795
Alw44I GTGCAC 1 cut(s) 876
AlwI GGATC 1 cut(s) 390
AoxI GGCC 1 cut(s) 808
ApaLI GTGCAC 1 cut(s) 876
ApeKI GCWGC 4 cut(s) 249, 623, 1124, 1445
ApoI RAATTY 3 cut(s) 185, 314, 1036
AseI ATTAAT 1 cut(s) 1374
Asp700I GAANNNNTTC 1 cut(s) 199
AspLEI GCGC 3 cut(s) 298, 358, 370
AspS9I GGNCC 4 cut(s) 151, 517, 809, 1262
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 2 cut(s) 181, 1213
AsuII TTCGAA 1 cut(s) 89
AvaII GGWCC 3 cut(s) 151, 517, 1262
BaeGI GKGCMC 1 cut(s) 880
BanII GRGCYC 2 cut(s) 616, 1496
BauI CACGAG 2 cut(s) 426, 851
Bbv12I GWGCWC 1 cut(s) 880
BbvI GCAGC 4 cut(s) 261, 610, 1111, 1432
BccI CCATC 4 cut(s) 426, 628, 684, 1150
BceAI ACGGC 7 cut(s) 246, 294, 309, 1124, 1220, 1308, 1319
BciT130I CCWGG 3 cut(s) 1266, 1321, 1502
BciVI GTATCC 2 cut(s) 473, 1163
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 2 cut(s) 780, 795
BfaI CTAG 1 cut(s) 714
BfoI RGCGCY 1 cut(s) 371
BfuI GTATCC 2 cut(s) 473, 1163
BisI GCNGC 6 cut(s) 250, 282, 594, 624, 1125, 1446
BlsI GCNGC 6 cut(s) 251, 283, 595, 625, 1126, 1447
Bme1390I CCNGG 4 cut(s) 163, 1266, 1321, 1502
Bme18I GGWCC 3 cut(s) 151, 517, 1262
BmgT120I GGNCC 4 cut(s) 151, 517, 809, 1262
BmiI GGNNCC 2 cut(s) 839, 1495
BmrFI CCNGG 4 cut(s) 163, 1266, 1321, 1502
BmsI GCATC 6 cut(s) 244, 339, 999, 1068, 1227, 1322
BpmI CTGGAG 1 cut(s) 1590
Bpu14I TTCGAA 1 cut(s) 89
BpuEI CTTGAG 2 cut(s) 777, 935
BpuMI CCSGG 1 cut(s) 163
Bsa29I ATCGAT 1 cut(s) 232
BsaBI GATNNNNATC 1 cut(s) 888
BsaHI GRCGYC 1 cut(s) 994
BsaJI CCNNGG 4 cut(s) 64, 587, 1145, 1501
BsaWI WCCGGW 4 cut(s) 191, 268, 449, 1403
BsaXI ACNNNNNCTCC 2 cut(s) 1563, 1593
Bsc4I CCNNNNNNNGG 5 cut(s) 268, 500, 592, 989, 1146
Bse1I ACTGG 1 cut(s) 1573
Bse3DI GCAATG 3 cut(s) 382, 738, 1173
Bse8I GATNNNNATC 1 cut(s) 888
BseBI CCWGG 3 cut(s) 1266, 1321, 1502
BseCI ATCGAT 1 cut(s) 232
BseDI CCNNGG 4 cut(s) 64, 587, 1145, 1501
BseGI GGATG 4 cut(s) 437, 889, 1083, 1509
BseJI GATNNNNATC 1 cut(s) 888
BseLI CCNNNNNNNGG 5 cut(s) 268, 500, 592, 989, 1146
BseMI GCAATG 3 cut(s) 382, 738, 1173
BseMII CTCAG 2 cut(s) 118, 908
BseNI ACTGG 1 cut(s) 1573
BseSI GKGCMC 1 cut(s) 880
BseXI GCAGC 4 cut(s) 261, 610, 1111, 1432
BseYI CCCAGC 2 cut(s) 1050, 1433
Bsh1236I CGCG 2 cut(s) 356, 1231
BshFI GGCC 1 cut(s) 810
BshVI ATCGAT 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 880
BsiSI CCGG 7 cut(s) 125, 139, 162, 192, 269, 450, 1404
BslFI GGGAC 1 cut(s) 1450
BslI CCNNNNNNNGG 5 cut(s) 268, 500, 592, 989, 1146
BsmAI GTCTC 2 cut(s) 780, 795
BsmFI GGGAC 1 cut(s) 1450
BsnI GGCC 1 cut(s) 810
Bsp119I TTCGAA 1 cut(s) 89
Bsp1286I GDGCHC 3 cut(s) 616, 880, 1496
Bsp143I GATC 5 cut(s) 70, 133, 208, 382, 883
Bsp19I CCATGG 2 cut(s) 64, 1145
BspACI CCGC 5 cut(s) 281, 284, 380, 593, 719
BspANI GGCC 1 cut(s) 810
BspCNI CTCAG 2 cut(s) 117, 907
BspDI ATCGAT 1 cut(s) 232
BspFNI CGCG 2 cut(s) 356, 1231
BspLI GGNNCC 2 cut(s) 839, 1495
BspPI GGATC 1 cut(s) 390
BspQI GCTCTTC 2 cut(s) 621, 666
BspT104I TTCGAA 1 cut(s) 89
BsrDI GCAATG 3 cut(s) 382, 738, 1173
BsrI ACTGG 1 cut(s) 1573
BssECI CCNNGG 4 cut(s) 64, 587, 1145, 1501
BssMI GATC 5 cut(s) 70, 133, 208, 382, 883
BssNI GRCGYC 1 cut(s) 994
BssSI CACGAG 2 cut(s) 426, 851
BssT1I CCWWGG 3 cut(s) 64, 587, 1145
Bst2BI CACGAG 2 cut(s) 426, 851
Bst2UI CCWGG 3 cut(s) 1266, 1321, 1502
Bst4CI ACNGT 2 cut(s) 521, 1462
Bst6I CTCTTC 2 cut(s) 621, 666
BstACI GRCGYC 1 cut(s) 994
BstBI TTCGAA 1 cut(s) 89
BstC8I GCNNGC 3 cut(s) 259, 354, 360
BstDEI CTNAG 3 cut(s) 104, 894, 1325
BstDSI CCRYGG 2 cut(s) 64, 1145
BstF5I GGATG 4 cut(s) 437, 889, 1083, 1509
BstFNI CGCG 2 cut(s) 356, 1231
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 3 cut(s) 298, 358, 370
BstKTI GATC 5 cut(s) 73, 136, 211, 385, 886
BstMAI GTCTC 2 cut(s) 780, 795
BstMBI GATC 5 cut(s) 70, 133, 208, 382, 883
BstMWI GCNNNNNNNGC 6 cut(s) 258, 353, 620, 629, 856, 934
BstNI CCWGG 3 cut(s) 1266, 1321, 1502
BstSCI CCNGG 4 cut(s) 161, 1264, 1319, 1500
BstSLI GKGCMC 1 cut(s) 880
BstUI CGCG 2 cut(s) 356, 1231
BstV1I GCAGC 4 cut(s) 261, 610, 1111, 1432
Bsu15I ATCGAT 1 cut(s) 232
BsuI GTATCC 2 cut(s) 473, 1163
BsuRI GGCC 1 cut(s) 810
BsuTUI ATCGAT 1 cut(s) 232
BtgI CCRYGG 2 cut(s) 64, 1145
BtgZI GCGATG 3 cut(s) 267, 362, 647
BtsCI GGATG 4 cut(s) 437, 889, 1083, 1509
Cac8I GCNNGC 3 cut(s) 259, 354, 360
CfoI GCGC 3 cut(s) 298, 358, 370
Cfr13I GGNCC 4 cut(s) 151, 517, 809, 1262
ClaI ATCGAT 1 cut(s) 232
CseI GACGC 1 cut(s) 1002
Csp6I GTAC 3 cut(s) 159, 264, 1212
CviQI GTAC 3 cut(s) 159, 264, 1212
DdeI CTNAG 3 cut(s) 104, 894, 1325
DpnI GATC 5 cut(s) 72, 135, 210, 384, 885
DpnII GATC 5 cut(s) 70, 133, 208, 382, 883
Eam1104I CTCTTC 2 cut(s) 621, 666
EarI CTCTTC 2 cut(s) 621, 666
EciI GGCGGA 1 cut(s) 299
Eco130I CCWWGG 3 cut(s) 64, 587, 1145
Eco24I GRGCYC 2 cut(s) 616, 1496
Eco47I GGWCC 3 cut(s) 151, 517, 1262
Eco57I CTGAAG 2 cut(s) 669, 1364
EcoRII CCWGG 3 cut(s) 1264, 1319, 1500
EcoT14I CCWWGG 3 cut(s) 64, 587, 1145
EcoT22I ATGCAT 1 cut(s) 1047
EcoT38I GRGCYC 2 cut(s) 616, 1496
ErhI CCWWGG 3 cut(s) 64, 587, 1145
FalI AAGNNNNNCTT 2 cut(s) 229, 261
FaqI GGGAC 1 cut(s) 1450
Fnu4HI GCNGC 6 cut(s) 250, 282, 594, 624, 1125, 1446
FokI GGATG 4 cut(s) 444, 876, 1090, 1496
FriOI GRGCYC 2 cut(s) 616, 1496
Fsp4HI GCNGC 6 cut(s) 250, 282, 594, 624, 1125, 1446
FspBI CTAG 1 cut(s) 714
GlaI GCGC 3 cut(s) 297, 357, 369
GluI GCNGC 6 cut(s) 250, 282, 594, 624, 1125, 1446
GsaI CCCAGC 2 cut(s) 1054, 1437
GsuI CTGGAG 1 cut(s) 1590
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 1 cut(s) 810
HapII CCGG 7 cut(s) 125, 139, 162, 192, 269, 450, 1404
HgaI GACGC 1 cut(s) 1002
HhaI GCGC 3 cut(s) 298, 358, 370
Hin1I GRCGYC 1 cut(s) 994
Hin6I GCGC 3 cut(s) 296, 356, 368
HinP1I GCGC 3 cut(s) 296, 356, 368
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 6 cut(s) 112, 168, 333, 765, 1066, 1506
HpaII CCGG 7 cut(s) 125, 139, 162, 192, 269, 450, 1404
HphI GGTGA 2 cut(s) 181, 1213
Hpy166II GTNNAC 6 cut(s) 175, 266, 291, 517, 878, 1111
Hpy188I TCNGA 5 cut(s) 313, 328, 897, 910, 1192
Hpy188III TCNNGA 3 cut(s) 106, 1382, 1394
Hpy8I GTNNAC 6 cut(s) 175, 266, 291, 517, 878, 1111
Hpy99I CGWCG 3 cut(s) 209, 328, 1294
HpyAV CCTTC 4 cut(s) 644, 1007, 1507, 1523
HpyCH4III ACNGT 2 cut(s) 521, 1462
HpyCH4IV ACGT 3 cut(s) 323, 709, 869
HpyCH4V TGCA 9 cut(s) 249, 375, 626, 731, 866, 878, 1034, 1045, 1243
HpyF10VI GCNNNNNNNGC 6 cut(s) 258, 353, 620, 629, 856, 934
HpyF3I CTNAG 3 cut(s) 104, 894, 1325
HpySE526I ACGT 3 cut(s) 323, 709, 869
Hsp92I GRCGYC 1 cut(s) 994
HspAI GCGC 3 cut(s) 296, 356, 368
Kzo9I GATC 5 cut(s) 70, 133, 208, 382, 883
LguI GCTCTTC 2 cut(s) 621, 666
LmnI GCTCC 5 cut(s) 127, 435, 1396, 1466, 1499
Lsp1109I GCAGC 4 cut(s) 261, 610, 1111, 1432
LweI GCATC 6 cut(s) 244, 339, 999, 1068, 1227, 1322
MaeI CTAG 1 cut(s) 714
MaeII ACGT 3 cut(s) 323, 709, 869
MaeIII GTNAC 1 cut(s) 5
MalI GATC 5 cut(s) 72, 135, 210, 384, 885
MboI GATC 5 cut(s) 70, 133, 208, 382, 883
MboII GAAGA 5 cut(s) 18, 284, 608, 653, 1370
MfeI CAATTG 2 cut(s) 30, 495
MhlI GDGCHC 3 cut(s) 616, 880, 1496
MlyI GAGTC 3 cut(s) 177, 342, 759
MmeI TCCRAC 3 cut(s) 306, 1170, 1444
MnlI CCTC 8 cut(s) 102, 164, 234, 423, 791, 903, 993, 1124
Mph1103I ATGCAT 1 cut(s) 1047
MroXI GAANNNNTTC 1 cut(s) 199
MseI TTAA 5 cut(s) 236, 485, 1226, 1344, 1374
MspI CCGG 7 cut(s) 125, 139, 162, 192, 269, 450, 1404
MspR9I CCNGG 4 cut(s) 163, 1266, 1321, 1502
MunI CAATTG 2 cut(s) 30, 495
MvaI CCWGG 3 cut(s) 1266, 1321, 1502
MvnI CGCG 2 cut(s) 356, 1231
MwoI GCNNNNNNNGC 6 cut(s) 258, 353, 620, 629, 856, 934
NciI CCSGG 1 cut(s) 163
NcoI CCATGG 2 cut(s) 64, 1145
NdeII GATC 5 cut(s) 70, 133, 208, 382, 883
NlaIV GGNNCC 2 cut(s) 839, 1495
NsiI ATGCAT 1 cut(s) 1047
NspV TTCGAA 1 cut(s) 89
PciSI GCTCTTC 2 cut(s) 621, 666
PdmI GAANNNNTTC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 112, 1066, 1506
PflMI CCANNNNNTGG 1 cut(s) 500
PfoI TCCNGGA 1 cut(s) 1264
PkrI GCNGC 6 cut(s) 251, 283, 595, 625, 1126, 1447
PleI GAGTC 3 cut(s) 176, 341, 759
PpsI GAGTC 3 cut(s) 176, 341, 759
PshBI ATTAAT 1 cut(s) 1374
PsiI TTATAA 1 cut(s) 320
Psp1406I AACGTT 1 cut(s) 709
Psp6I CCWGG 3 cut(s) 1264, 1319, 1500
PspFI CCCAGC 2 cut(s) 1050, 1433
PspGI CCWGG 3 cut(s) 1264, 1319, 1500
PspN4I GGNNCC 2 cut(s) 839, 1495
PspPI GGNCC 4 cut(s) 151, 517, 809, 1262
RsaI GTAC 3 cut(s) 160, 265, 1213
RsaNI GTAC 3 cut(s) 159, 264, 1212
SapI GCTCTTC 2 cut(s) 621, 666
SaqAI TTAA 5 cut(s) 236, 485, 1226, 1344, 1374
SatI GCNGC 6 cut(s) 250, 282, 594, 624, 1125, 1446
Sau3AI GATC 5 cut(s) 70, 133, 208, 382, 883
Sau96I GGNCC 4 cut(s) 151, 517, 809, 1262
SchI GAGTC 3 cut(s) 177, 342, 759
ScrFI CCNGG 4 cut(s) 163, 1266, 1321, 1502
SduI GDGCHC 3 cut(s) 616, 880, 1496
SfaNI GCATC 6 cut(s) 244, 339, 999, 1068, 1227, 1322
SfuI TTCGAA 1 cut(s) 89
SinI GGWCC 3 cut(s) 151, 517, 1262
SmlI CTYRAG 2 cut(s) 792, 914
SmoI CTYRAG 2 cut(s) 792, 914
SsiI CCGC 5 cut(s) 281, 284, 380, 593, 719
SspI AATATT 2 cut(s) 1400, 1527
SspMI CTAG 1 cut(s) 714
StyD4I CCNGG 4 cut(s) 161, 1264, 1319, 1500
StyI CCWWGG 3 cut(s) 64, 587, 1145
TaaI ACNGT 2 cut(s) 521, 1462
TaiI ACGT 3 cut(s) 326, 712, 872
TaqI TCGA 6 cut(s) 46, 73, 89, 207, 232, 886
TauI GCSGC 2 cut(s) 284, 596
TfiI GAWTC 3 cut(s) 112, 1066, 1506
Tru1I TTAA 5 cut(s) 236, 485, 1226, 1344, 1374
Tru9I TTAA 5 cut(s) 236, 485, 1226, 1344, 1374
TseI GCWGC 4 cut(s) 249, 623, 1124, 1445
TspDTI ATGAA 4 cut(s) 686, 1062, 1246, 1498
TspGWI ACGGA 3 cut(s) 105, 193, 388
Van91I CCANNNNNTGG 1 cut(s) 500
VneI GTGCAC 1 cut(s) 876
VpaK11BI GGWCC 3 cut(s) 151, 517, 1262
VspI ATTAAT 1 cut(s) 1374
XapI RAATTY 3 cut(s) 185, 314, 1036
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 1 cut(s) 714
Zsp2I ATGCAT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.