Rh5DG479300

anion transporter 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004615
Physical Location & Seq
Reverse (-)
75665154 .. 75668387
3234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG479300.1

Sequence Viewer

Length: 1584 bp
ATGAACTCCACAATCCGCACTTCTTATCTCTCTCTTTCCCGCGGCCGGAAAATTCCCAAACCGGAATCGCCGAGCCTCCAACTACCTCCTCGTTCCGTCAACTCGCCGAACCGTTGCAACGGTTTCCGAAACGTTTCGTCGGCCAATTCGCTGCTGAGAATTGGACTCCGGCATGACTCGGCTGACCTCCGCGGTGTTCAATCGAGACGAGTTAGGGTTTCTTCCAAGAACGCTCAGTTCGGCTCGTTCTCGGACGGGAACGAAGCTCAGGCGCCGAGCTTCGGGGAGTTTATTACATCGGAGAGAGTCAAAGTGGTGGCGATGCTCGCTTTGGCTCTGGCTCTGTGCAATGCCGACCGTGTTGTGATGTCGGTGGCGATTGTGCCCTTATCGCTCTCTAATGGTTGGAGCCCATCGTTTGCTGGTATTGTTCAGTCGTCTTTCCTGTGGGGCTACCTTGTATCTCCGATAGCTGGAGGGACTCTTGTGGACTACTATGGTGGTAAGGTAGTCATGGCTTGGGGGGTGGCTTTGTGGTCTATGGCTACCTTCCTTACACCGTGGGCTGCTGAAACTTCTCTATGGCTTCTCCTCGCAATGAGAGCTCTCCTTGGCATTGCGGAAGGCGTGGCTCTTCCTTGCATGAACAACATGGTAGCAAGATGGTTTCCTAAGACAGAACGATCCAGGGCTGTTGGAATTGCAATGGCTGGATTTCAGCTTGGAAGTTCAGTAGGACTCATGCTATCTCCGATTCTCATGTCACAAGGTGGTGTATTTGGACCATTTGTGATTTTTGGTTTATCTGGATTTCTTTGGGTTTTGGTATGGTTATCTGCAACATCAAGTACTCCTGACCGCAGTCCTCAGATATCAAAATATGAATTGGATTACATACAGAGCGAGAGGCAGAAATCCTTTCTGGGGGAAAATAAACCTAAGACAACCAAAGTGATCCCTCCTTTCAGGCGCTTGCTTTCTAAACCGCCCACCTGGGCCTTAATAGTTGCAAATGCCATGCATAGCTGGGGTTTTTTCGTTTTTCTTTCCTGGATGCCCATCTACTTCAACTCCGTGTATCATGTTGACCTGAGACAGGCAGCTTGGTTTAGTGCTGTTCCATGGACTGTCATGGCAGTCATGGGATATATTGGTGGTTTGTGGTCGGACATAATGATACGAAATGGTACAAGTGTCACTTTGACTCGAAAGATCATGCAGTCGATCGGTTTCATAGGTCCTGGGATTGCTCTTGTAGGTTTAACTACAGCAAAAAGTCCATCAGTTGCATCTGCTTGGCTTACTTTAGCAGTTGGACTTAAATCCTTTAGTCACTCTGGCTTTCTTGTGAATCTTCAGGAGATCGCTCCCCAGTACTCTGGTGTTTTACATGGAATATCAAATACTGCCGGTACATTTGCTGCTATTTTGGGAACAGTTGGAGCTGGTTTTTTTGTCGAATTGGTGGGTTCTTTCCAAGGATTTCTGTTGTTCACATCACTGTTATATATTCTTGCTGCCCTTTTCTACATCCTATTTTCTACCGGGGAGAGAGTAGATTTTGATGAACCAACTCCAGTATGA

Protein Analysis

527

Amino Acids

57.2

Weight (kDa)

9.91

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 109 - 479 5.9e-52 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AccII CGCG 2 cut(s) 42, 192
AciI CCGC 8 cut(s) 16, 40, 42, 190, 192, 620, 859, 986
AclI AACGTT 1 cut(s) 132
AclWI GGATC 2 cut(s) 678, 949
AcoI YGGCCR 2 cut(s) 43, 141
AcsI RAATTY 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 1340
AcyI GRCGYC 1 cut(s) 272
AdeI CACNNNGTG 1 cut(s) 770
AfaI GTAC 4 cut(s) 850, 1189, 1376, 1414
AfiI CCNNNNNNNGG 5 cut(s) 45, 281, 473, 924, 1096
AgsI TTSAA 2 cut(s) 200, 1069
AjnI CCWGG 4 cut(s) 686, 992, 1049, 1240
AluBI AGCT 8 cut(s) 266, 279, 473, 605, 721, 1026, 1103, 1445
AluI AGCT 8 cut(s) 266, 279, 473, 605, 721, 1026, 1103, 1445
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 2 cut(s) 199, 1087
AlwI GGATC 2 cut(s) 678, 949
AoxI GGCC 3 cut(s) 43, 141, 996
ApeKI GCWGC 5 cut(s) 151, 566, 1100, 1421, 1517
ApoI RAATTY 1 cut(s) 51
Asp700I GAANNNNTTC 1 cut(s) 133
AspLEI GCGC 2 cut(s) 274, 972
AspS9I GGNCC 3 cut(s) 782, 996, 1238
AsuC2I CCSGG 1 cut(s) 1546
AvaII GGWCC 2 cut(s) 782, 1238
BaeGI GKGCMC 1 cut(s) 387
BaeI ACNNNNGTAYC 2 cut(s) 1179, 1212
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 2 cut(s) 413, 607
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 5 cut(s) 138, 553, 1112, 1408, 1504
BccI CCATC 4 cut(s) 421, 657, 1067, 1288
BciT130I CCWGG 4 cut(s) 688, 994, 1051, 1242
BcnI CCSGG 1 cut(s) 1546
BcoDI GTCTC 2 cut(s) 199, 1087
BfmI CTRYAG 1 cut(s) 1266
BfoI RGCGCY 2 cut(s) 275, 973
BisI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
BlsI GCNGC 6 cut(s) 44, 153, 568, 1102, 1423, 1519
BmcAI AGTACT 2 cut(s) 850, 1376
Bme1390I CCNGG 5 cut(s) 688, 994, 1051, 1242, 1546
Bme18I GGWCC 2 cut(s) 782, 1238
BmgT120I GGNCC 3 cut(s) 782, 996, 1238
BmiI GGNNCC 2 cut(s) 273, 410
BmrFI CCNGG 5 cut(s) 688, 994, 1051, 1242, 1546
BmrI ACTGGG 1 cut(s) 1366
BmsI GCATC 3 cut(s) 312, 1044, 1298
BmuI ACTGGG 1 cut(s) 1366
BoxI GACNNNNGTC 1 cut(s) 861
BpmI CTGGAG 2 cut(s) 495, 1560
Bpu10I CCTNAGC 1 cut(s) 267
BpuMI CCSGG 1 cut(s) 1546
BsaBI GATNNNNATC 1 cut(s) 1058
BsaHI GRCGYC 1 cut(s) 272
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 2 cut(s) 1055, 1085
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 281, 473, 924, 1096
Bse118I RCCGGY 1 cut(s) 1409
Bse1I ACTGG 2 cut(s) 1372, 1577
Bse3DI GCAATG 4 cut(s) 355, 603, 615, 711
Bse8I GATNNNNATC 1 cut(s) 1058
BseBI CCWGG 4 cut(s) 688, 994, 1051, 1242
BseGI GGATG 2 cut(s) 1059, 1530
BseJI GATNNNNATC 1 cut(s) 1058
BseLI CCNNNNNNNGG 5 cut(s) 45, 281, 473, 924, 1096
BseMI GCAATG 4 cut(s) 355, 603, 615, 711
BseMII CTCAG 5 cut(s) 146, 248, 281, 881, 1082
BseNI ACTGG 2 cut(s) 1372, 1577
BseRI GAGGAG 2 cut(s) 78, 581
BseSI GKGCMC 1 cut(s) 387
BseX3I CGGCCG 1 cut(s) 43
BseXI GCAGC 5 cut(s) 138, 553, 1112, 1408, 1504
BseYI CCCAGC 1 cut(s) 1026
Bsh1236I CGCG 2 cut(s) 42, 192
Bsh1285I CGRYCG 3 cut(s) 46, 358, 1227
BshFI GGCC 3 cut(s) 45, 143, 998
BshNI GGYRCC 1 cut(s) 271
BsiEI CGRYCG 3 cut(s) 46, 358, 1227
BsiHKAI GWGCWC 1 cut(s) 607
BsiSI CCGG 5 cut(s) 46, 62, 169, 1410, 1545
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 5 cut(s) 45, 281, 473, 924, 1096
BsmAI GTCTC 2 cut(s) 199, 1087
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 3 cut(s) 45, 143, 998
Bsp1286I GDGCHC 3 cut(s) 387, 413, 607
Bsp143I GATC 5 cut(s) 683, 954, 1212, 1224, 1362
Bsp19I CCATGG 1 cut(s) 1121
BspACI CCGC 8 cut(s) 16, 40, 42, 190, 192, 620, 859, 986
BspANI GGCC 3 cut(s) 45, 143, 998
BspCNI CTCAG 5 cut(s) 147, 247, 280, 880, 1083
BspFNI CGCG 2 cut(s) 42, 192
BspLI GGNNCC 2 cut(s) 273, 410
BspPI GGATC 2 cut(s) 678, 949
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 271
BsrDI GCAATG 4 cut(s) 355, 603, 615, 711
BsrFI RCCGGY 1 cut(s) 1409
BsrI ACTGG 2 cut(s) 1372, 1577
BssAI RCCGGY 1 cut(s) 1409
BssMI GATC 5 cut(s) 683, 954, 1212, 1224, 1362
BssNI GRCGYC 1 cut(s) 272
BssT1I CCWWGG 3 cut(s) 610, 1121, 1477
Bst2UI CCWGG 4 cut(s) 688, 994, 1051, 1242
Bst4CI ACNGT 7 cut(s) 113, 122, 359, 561, 1129, 1438, 1503
Bst6I CTCTTC 1 cut(s) 639
BstACI GRCGYC 1 cut(s) 272
BstC8I GCNNGC 2 cut(s) 327, 974
BstDEI CTNAG 7 cut(s) 155, 234, 267, 672, 867, 939, 1091
BstDSI CCRYGG 4 cut(s) 40, 190, 560, 1121
BstENI CCTNNNNNAGG 1 cut(s) 1094
BstF5I GGATG 2 cut(s) 1059, 1530
BstFNI CGCG 2 cut(s) 42, 192
BstH2I RGCGCY 2 cut(s) 275, 973
BstHHI GCGC 2 cut(s) 274, 972
BstKTI GATC 5 cut(s) 686, 957, 1215, 1227, 1365
BstMAI GTCTC 2 cut(s) 199, 1087
BstMBI GATC 5 cut(s) 683, 954, 1212, 1224, 1362
BstMCI CGRYCG 3 cut(s) 46, 358, 1227
BstMWI GCNNNNNNNGC 3 cut(s) 326, 391, 602
BstNI CCWGG 4 cut(s) 688, 994, 1051, 1242
BstPAI GACNNNNGTC 1 cut(s) 861
BstSCI CCNGG 5 cut(s) 686, 992, 1049, 1240, 1544
BstSFI CTRYAG 1 cut(s) 1266
BstSLI GKGCMC 1 cut(s) 387
BstUI CGCG 2 cut(s) 42, 192
BstV1I GCAGC 5 cut(s) 138, 553, 1112, 1408, 1504
BstXI CCANNNNNNTGG 1 cut(s) 1379
BstZI CGGCCG 1 cut(s) 43
BsuRI GGCC 3 cut(s) 45, 143, 998
BtgI CCRYGG 4 cut(s) 40, 190, 560, 1121
BtgZI GCGATG 1 cut(s) 335
BtsCI GGATG 2 cut(s) 1059, 1530
BtsIMutI CAGTG 1 cut(s) 1499
Cac8I GCNNGC 2 cut(s) 327, 974
CfoI GCGC 2 cut(s) 274, 972
Cfr10I RCCGGY 1 cut(s) 1409
Cfr13I GGNCC 3 cut(s) 782, 996, 1238
Cfr42I CCGCGG 2 cut(s) 43, 193
Csp6I GTAC 4 cut(s) 849, 1188, 1375, 1413
CviQI GTAC 4 cut(s) 849, 1188, 1375, 1413
DdeI CTNAG 7 cut(s) 155, 234, 267, 672, 867, 939, 1091
DinI GGCGCC 1 cut(s) 273
DpnI GATC 5 cut(s) 685, 956, 1214, 1226, 1364
DpnII GATC 5 cut(s) 683, 954, 1212, 1224, 1362
DraIII CACNNNGTG 1 cut(s) 770
EaeI YGGCCR 2 cut(s) 43, 141
EagI CGGCCG 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 639
EarI CTCTTC 1 cut(s) 639
Ecl136II GAGCTC 1 cut(s) 605
EclXI CGGCCG 1 cut(s) 43
Eco130I CCWWGG 3 cut(s) 610, 1121, 1477
Eco24I GRGCYC 2 cut(s) 413, 607
Eco32I GATATC 1 cut(s) 873
Eco47I GGWCC 2 cut(s) 782, 1238
Eco52I CGGCCG 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 605
Eco57I CTGAAG 1 cut(s) 1340
EcoICRI GAGCTC 1 cut(s) 605
EcoNI CCTNNNNNAGG 1 cut(s) 1094
EcoO109I RGGNCCY 1 cut(s) 1238
EcoRII CCWGG 4 cut(s) 686, 992, 1049, 1240
EcoRV GATATC 1 cut(s) 873
EcoT14I CCWWGG 3 cut(s) 610, 1121, 1477
EcoT22I ATGCAT 1 cut(s) 1023
EcoT38I GRGCYC 2 cut(s) 413, 607
EgeI GGCGCC 1 cut(s) 273
EheI GGCGCC 1 cut(s) 273
ErhI CCWWGG 3 cut(s) 610, 1121, 1477
Esp3I CGTCTC 1 cut(s) 199
FalI AAGNNNNNCTT 2 cut(s) 1183, 1215
FaqI GGGAC 1 cut(s) 493
FauI CCCGC 1 cut(s) 47
Fnu4HI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
FokI GGATG 2 cut(s) 1066, 1517
FriOI GRGCYC 2 cut(s) 413, 607
Fsp4HI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
GlaI GCGC 2 cut(s) 273, 971
GluI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
GsaI CCCAGC 1 cut(s) 1030
GsuI CTGGAG 2 cut(s) 495, 1560
HaeII RGCGCY 2 cut(s) 275, 973
HaeIII GGCC 3 cut(s) 45, 143, 998
HapII CCGG 5 cut(s) 46, 62, 169, 1410, 1545
HhaI GCGC 2 cut(s) 274, 972
Hin1I GRCGYC 1 cut(s) 272
Hin6I GCGC 2 cut(s) 272, 970
HinP1I GCGC 2 cut(s) 272, 970
HincII GTYRAC 2 cut(s) 100, 1087
HindII GTYRAC 2 cut(s) 100, 1087
HinfI GANTC 9 cut(s) 65, 165, 176, 306, 481, 738, 754, 1204, 1351
HpaII CCGG 5 cut(s) 46, 62, 169, 1410, 1545
Hpy166II GTNNAC 4 cut(s) 100, 490, 1087, 1494
Hpy188I TCNGA 7 cut(s) 128, 253, 301, 468, 753, 870, 1168
Hpy188III TCNNGA 4 cut(s) 204, 807, 854, 1358
Hpy8I GTNNAC 4 cut(s) 100, 490, 1087, 1494
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 2 cut(s) 559, 617
HpyCH4III ACNGT 7 cut(s) 113, 122, 359, 561, 1129, 1438, 1503
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 9 cut(s) 117, 348, 642, 704, 839, 1010, 1021, 1219, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 391, 602
HpyF3I CTNAG 7 cut(s) 155, 234, 267, 672, 867, 939, 1091
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 272
HspAI GCGC 2 cut(s) 272, 970
KasI GGCGCC 1 cut(s) 271
KspI CCGCGG 2 cut(s) 43, 193
Kzo9I GATC 5 cut(s) 683, 954, 1212, 1224, 1362
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 3 cut(s) 408, 1372, 1442
Lsp1109I GCAGC 5 cut(s) 138, 553, 1112, 1408, 1504
LweI GCATC 3 cut(s) 312, 1044, 1298
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 762, 1195, 1331
MalI GATC 5 cut(s) 685, 956, 1214, 1226, 1364
MboI GATC 5 cut(s) 683, 954, 1212, 1224, 1362
MboII GAAGA 3 cut(s) 213, 626, 1346
MhlI GDGCHC 3 cut(s) 387, 413, 607
MluCI AATT 6 cut(s) 51, 145, 159, 699, 884, 1460
Mly113I GGCGCC 1 cut(s) 272
MlyI GAGTC 6 cut(s) 159, 170, 315, 475, 732, 1198
MmeI TCCRAC 6 cut(s) 103, 386, 676, 1146, 1294, 1420
MnlI CCTC 9 cut(s) 86, 96, 99, 197, 470, 602, 876, 900, 969
Mph1103I ATGCAT 1 cut(s) 1023
MroXI GAANNNNTTC 1 cut(s) 133
MseI TTAA 3 cut(s) 1001, 1262, 1320
MspA1I CMGCKG 2 cut(s) 42, 192
MspI CCGG 5 cut(s) 46, 62, 169, 1410, 1545
MspR9I CCNGG 5 cut(s) 688, 994, 1051, 1242, 1546
MvaI CCWGG 4 cut(s) 688, 994, 1051, 1242
MvnI CGCG 2 cut(s) 42, 192
MwoI GCNNNNNNNGC 3 cut(s) 326, 391, 602
NarI GGCGCC 1 cut(s) 272
NciI CCSGG 1 cut(s) 1546
NcoI CCATGG 1 cut(s) 1121
NdeII GATC 5 cut(s) 683, 954, 1212, 1224, 1362
NlaIV GGNNCC 2 cut(s) 273, 410
NmeAIII GCCGAG 3 cut(s) 96, 158, 300
NmuCI GTSAC 3 cut(s) 762, 1195, 1331
NsiI ATGCAT 1 cut(s) 1023
PciSI GCTCTTC 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 3 cut(s) 65, 754, 1351
PfoI TCCNGGA 1 cut(s) 1049
PkrI GCNGC 6 cut(s) 44, 153, 568, 1102, 1423, 1519
Ple19I CGATCG 1 cut(s) 1227
PleI GAGTC 6 cut(s) 159, 170, 314, 475, 732, 1198
PluTI GGCGCC 1 cut(s) 275
PpsI GAGTC 6 cut(s) 159, 170, 314, 475, 732, 1198
PpuMI RGGWCCY 1 cut(s) 1238
PshAI GACNNNNGTC 1 cut(s) 861
Psp124BI GAGCTC 1 cut(s) 607
Psp1406I AACGTT 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 1238
Psp6I CCWGG 4 cut(s) 686, 992, 1049, 1240
PspFI CCCAGC 1 cut(s) 1026
PspGI CCWGG 4 cut(s) 686, 992, 1049, 1240
PspN4I GGNNCC 2 cut(s) 273, 410
PspPI GGNCC 3 cut(s) 782, 996, 1238
PspPPI RGGWCCY 1 cut(s) 1238
PvuI CGATCG 1 cut(s) 1227
RsaI GTAC 4 cut(s) 850, 1189, 1376, 1414
RsaNI GTAC 4 cut(s) 849, 1188, 1375, 1413
SacI GAGCTC 1 cut(s) 607
SacII CCGCGG 2 cut(s) 43, 193
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 3 cut(s) 1001, 1262, 1320
SatI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
Sau3AI GATC 5 cut(s) 683, 954, 1212, 1224, 1362
Sau96I GGNCC 3 cut(s) 782, 996, 1238
ScaI AGTACT 2 cut(s) 850, 1376
SchI GAGTC 6 cut(s) 159, 170, 315, 475, 732, 1198
ScrFI CCNGG 5 cut(s) 688, 994, 1051, 1242, 1546
SduI GDGCHC 3 cut(s) 387, 413, 607
SfaNI GCATC 3 cut(s) 312, 1044, 1298
SfcI CTRYAG 1 cut(s) 1266
SfoI GGCGCC 1 cut(s) 273
Sfr303I CCGCGG 2 cut(s) 43, 193
SgrBI CCGCGG 2 cut(s) 43, 193
SinI GGWCC 2 cut(s) 782, 1238
Sse9I AATT 6 cut(s) 51, 145, 159, 699, 884, 1460
SsiI CCGC 8 cut(s) 16, 40, 42, 190, 192, 620, 859, 986
SspDI GGCGCC 1 cut(s) 271
SstI GAGCTC 1 cut(s) 607
StyD4I CCNGG 5 cut(s) 686, 992, 1049, 1240, 1544
StyI CCWWGG 3 cut(s) 610, 1121, 1477
TaaI ACNGT 7 cut(s) 113, 122, 359, 561, 1129, 1438, 1503
TaiI ACGT 1 cut(s) 135
TaqI TCGA 4 cut(s) 203, 1207, 1223, 1458
TasI AATT 6 cut(s) 51, 145, 159, 699, 884, 1460
TatI WGTACW 2 cut(s) 848, 1374
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 3 cut(s) 65, 754, 1351
Tru1I TTAA 3 cut(s) 1001, 1262, 1320
Tru9I TTAA 3 cut(s) 1001, 1262, 1320
TscAI CASTG 1 cut(s) 1506
TseFI GTSAC 3 cut(s) 762, 1195, 1331
TseI GCWGC 5 cut(s) 151, 566, 1100, 1421, 1517
Tsp45I GTSAC 3 cut(s) 762, 1195, 1331
TspDTI ATGAA 5 cut(s) 17, 659, 897, 1222, 1581
TspGWI ACGGA 2 cut(s) 85, 1063
TspRI CASTG 1 cut(s) 1506
VpaK11BI GGWCC 2 cut(s) 782, 1238
XagI CCTNNNNNAGG 1 cut(s) 1094
XapI RAATTY 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 133
ZrmI AGTACT 2 cut(s) 850, 1376
Zsp2I ATGCAT 1 cut(s) 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.