Rw5G041790

anion transporter 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004615
Physical Location & Seq
Reverse (-)
73469373 .. 73473172
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G041790.1

Sequence Viewer

Length: 1584 bp
ATGAACTCCACAATCCGCACTTCTTATCTCTCTCTTTCCCGCGGCCGGAAAATTCCCAAACCGGAATCGCCGAGCCTCCAACTACCTCCTCGTTCCGTCAACTCGCCGAACCGTTGCAACGGTTTCCGAAACGTTTCGTCGGCCAATTCGCTGCTGAGAATTGGACTCCGGCATGACTCGGCTGACCTCCGCGGTGTTCAATCGAGACGAGTTAGGGTTTCTTCCAAGAACGCTCAGTTCGGCTCGTTCTCGGACGGGAACGAAGCTCAGGCGCCGAGCTTCGGGGAGTTTATTACATCGGAGAGAGTCAAAGTGGTGGCGATGCTCGCTTTGGCTCTGGCTCTGTGCAATGCCGACCGTGTTGTGATGTCGGTGGCGATTGTGCCCTTGTCGCTCTCTAATGGTTGGAGCCCATCGTTTGCTGGTATTGTTCAGTCGTCTTTCCTGTGGGGCTACCTTGTATCTCCGATAGCTGGAGGGACTCTTGTGGACTACTATGGTGGTAAGGTAGTCATGGCTTGGGGGGTGGCTTTGTGGTCTATGGCTACCTTCCTTACACCGTGGGCTGCTGAAACTTCTCTATGGCTTCTCCTCGCAATGAGAGCTCTCCTTGGCATTGCGGAAGGCGTGGCTCTTCCTTGCATGAACAACATGGTAGCAAGATGGTTTCCTAAGACAGAACGATCCAGGGCTGTTGGAATTGCAATGGCTGGATTTCAGCTTGGAAGTTCAGTAGGACTCATGCTATCTCCGATTCTCATGTCACAAGGTGGTGTATTTGGACCATTTGTGATTTTTGGTTTATCTGGATTTCTTTGGGTTTTGGTATGGCTATCTGCAACATCAAGTACTCCTGACCGCAGTCCTCAGATATCAAAATATGAATTGGATTACATACAGAGCGAGAGGCAGAAATCCTTTCTGGGGGAAAATAAAGCTAAGACAACCAAAGTGATCCCTCCTTTCAGGCGCTTGCTTTCTAAACCGCCCACCTGGGCCTTAATAGTTGCAAATGCCATGCATAGCTGGGGTTTTTTCGTTTTTCTTTCCTGGATGCCCATCTACTTCAACTCCGTGTATCATGTTGACCTGAGACAAGCAGCTTGGTTTAGTGCTGTTCCATGGACTGTCATGGCAGTCATGGGATATATTGGTGGTTTGTGGTCGGACATAATGATACGAAATGGTACAAGTGTCACTTTGACTCGAAAGATCATGCAGTCGATCGGTTTCATAGGTCCTGGGATTGCTCTTGTAGGTTTAACTACAGCAAAAAGTCCATCAGTTGCATCTGCTTGGCTTACTTTAGCAGTTGGACTTAAATCCTTTAGTCACTCTGGCTTTCTTGTGAATCTTCAGGAGATCGCTCCCCAGTACTCTGGTGTTTTACATGGAATATCAAATACTGCCGGTACATTTGCTGCTATTTTGGGAACAGTTGGAGCTGGTTTTTTTGTCGAATTGGTGGGTTCTTTCCAAGGATTTCTGTTGTTCACATCACTGTTATATATTCTTGCTGCCCTTTTCTACATCCTATTTTCTACCGGGGAGAGAGTAGATTTTGATGAACCAACTCCAGTATGA

Protein Analysis

527

Amino Acids

57.17

Weight (kDa)

9.91

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 109 - 479 1e-51 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AccII CGCG 2 cut(s) 42, 192
AciI CCGC 8 cut(s) 16, 40, 42, 190, 192, 620, 859, 986
AclI AACGTT 1 cut(s) 132
AclWI GGATC 2 cut(s) 678, 949
AcoI YGGCCR 2 cut(s) 43, 141
AcsI RAATTY 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 1340
AcyI GRCGYC 1 cut(s) 272
AdeI CACNNNGTG 1 cut(s) 770
AfaI GTAC 4 cut(s) 850, 1189, 1376, 1414
AfiI CCNNNNNNNGG 4 cut(s) 45, 281, 473, 924
AgsI TTSAA 2 cut(s) 200, 1069
AjnI CCWGG 4 cut(s) 686, 992, 1049, 1240
AluBI AGCT 9 cut(s) 266, 279, 473, 605, 721, 938, 1026, 1103, 1445
AluI AGCT 9 cut(s) 266, 279, 473, 605, 721, 938, 1026, 1103, 1445
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 2 cut(s) 199, 1087
AlwI GGATC 2 cut(s) 678, 949
AoxI GGCC 3 cut(s) 43, 141, 996
ApeKI GCWGC 5 cut(s) 151, 566, 1100, 1421, 1517
ApoI RAATTY 1 cut(s) 51
Asp700I GAANNNNTTC 1 cut(s) 133
AspLEI GCGC 2 cut(s) 274, 972
AspS9I GGNCC 3 cut(s) 782, 996, 1238
AsuC2I CCSGG 1 cut(s) 1546
AvaII GGWCC 2 cut(s) 782, 1238
BaeGI GKGCMC 1 cut(s) 387
BaeI ACNNNNGTAYC 2 cut(s) 1179, 1212
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 2 cut(s) 413, 607
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 5 cut(s) 138, 553, 1112, 1408, 1504
BccI CCATC 4 cut(s) 421, 657, 1067, 1288
BciT130I CCWGG 4 cut(s) 688, 994, 1051, 1242
BcnI CCSGG 1 cut(s) 1546
BcoDI GTCTC 2 cut(s) 199, 1087
BfmI CTRYAG 1 cut(s) 1266
BfoI RGCGCY 2 cut(s) 275, 973
BisI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
BlsI GCNGC 6 cut(s) 44, 153, 568, 1102, 1423, 1519
BmcAI AGTACT 2 cut(s) 850, 1376
Bme1390I CCNGG 5 cut(s) 688, 994, 1051, 1242, 1546
Bme18I GGWCC 2 cut(s) 782, 1238
BmgT120I GGNCC 3 cut(s) 782, 996, 1238
BmiI GGNNCC 2 cut(s) 273, 410
BmrFI CCNGG 5 cut(s) 688, 994, 1051, 1242, 1546
BmrI ACTGGG 1 cut(s) 1366
BmsI GCATC 3 cut(s) 312, 1044, 1298
BmuI ACTGGG 1 cut(s) 1366
BoxI GACNNNNGTC 1 cut(s) 861
BpmI CTGGAG 2 cut(s) 495, 1560
Bpu10I CCTNAGC 1 cut(s) 267
BpuMI CCSGG 1 cut(s) 1546
BsaBI GATNNNNATC 1 cut(s) 1058
BsaHI GRCGYC 1 cut(s) 272
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 2 cut(s) 1055, 1085
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 281, 473, 924
Bse118I RCCGGY 1 cut(s) 1409
Bse1I ACTGG 2 cut(s) 1372, 1577
Bse3DI GCAATG 4 cut(s) 355, 603, 615, 711
Bse8I GATNNNNATC 1 cut(s) 1058
BseBI CCWGG 4 cut(s) 688, 994, 1051, 1242
BseGI GGATG 2 cut(s) 1059, 1530
BseJI GATNNNNATC 1 cut(s) 1058
BseLI CCNNNNNNNGG 4 cut(s) 45, 281, 473, 924
BseMI GCAATG 4 cut(s) 355, 603, 615, 711
BseMII CTCAG 5 cut(s) 146, 248, 281, 881, 1082
BseNI ACTGG 2 cut(s) 1372, 1577
BseRI GAGGAG 2 cut(s) 78, 581
BseSI GKGCMC 1 cut(s) 387
BseX3I CGGCCG 1 cut(s) 43
BseXI GCAGC 5 cut(s) 138, 553, 1112, 1408, 1504
BseYI CCCAGC 1 cut(s) 1026
Bsh1236I CGCG 2 cut(s) 42, 192
Bsh1285I CGRYCG 3 cut(s) 46, 358, 1227
BshFI GGCC 3 cut(s) 45, 143, 998
BshNI GGYRCC 1 cut(s) 271
BsiEI CGRYCG 3 cut(s) 46, 358, 1227
BsiHKAI GWGCWC 1 cut(s) 607
BsiSI CCGG 5 cut(s) 46, 62, 169, 1410, 1545
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 4 cut(s) 45, 281, 473, 924
BsmAI GTCTC 2 cut(s) 199, 1087
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 3 cut(s) 45, 143, 998
Bsp1286I GDGCHC 3 cut(s) 387, 413, 607
Bsp143I GATC 5 cut(s) 683, 954, 1212, 1224, 1362
Bsp19I CCATGG 1 cut(s) 1121
BspACI CCGC 8 cut(s) 16, 40, 42, 190, 192, 620, 859, 986
BspANI GGCC 3 cut(s) 45, 143, 998
BspCNI CTCAG 5 cut(s) 147, 247, 280, 880, 1083
BspFNI CGCG 2 cut(s) 42, 192
BspLI GGNNCC 2 cut(s) 273, 410
BspPI GGATC 2 cut(s) 678, 949
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 271
BsrDI GCAATG 4 cut(s) 355, 603, 615, 711
BsrFI RCCGGY 1 cut(s) 1409
BsrI ACTGG 2 cut(s) 1372, 1577
BssAI RCCGGY 1 cut(s) 1409
BssMI GATC 5 cut(s) 683, 954, 1212, 1224, 1362
BssNI GRCGYC 1 cut(s) 272
BssT1I CCWWGG 3 cut(s) 610, 1121, 1477
Bst2UI CCWGG 4 cut(s) 688, 994, 1051, 1242
Bst4CI ACNGT 7 cut(s) 113, 122, 359, 561, 1129, 1438, 1503
Bst6I CTCTTC 1 cut(s) 639
BstACI GRCGYC 1 cut(s) 272
BstC8I GCNNGC 2 cut(s) 327, 974
BstDEI CTNAG 7 cut(s) 155, 234, 267, 672, 867, 939, 1091
BstDSI CCRYGG 4 cut(s) 40, 190, 560, 1121
BstF5I GGATG 2 cut(s) 1059, 1530
BstFNI CGCG 2 cut(s) 42, 192
BstH2I RGCGCY 2 cut(s) 275, 973
BstHHI GCGC 2 cut(s) 274, 972
BstKTI GATC 5 cut(s) 686, 957, 1215, 1227, 1365
BstMAI GTCTC 2 cut(s) 199, 1087
BstMBI GATC 5 cut(s) 683, 954, 1212, 1224, 1362
BstMCI CGRYCG 3 cut(s) 46, 358, 1227
BstMWI GCNNNNNNNGC 3 cut(s) 326, 391, 602
BstNI CCWGG 4 cut(s) 688, 994, 1051, 1242
BstPAI GACNNNNGTC 1 cut(s) 861
BstSCI CCNGG 5 cut(s) 686, 992, 1049, 1240, 1544
BstSFI CTRYAG 1 cut(s) 1266
BstSLI GKGCMC 1 cut(s) 387
BstUI CGCG 2 cut(s) 42, 192
BstV1I GCAGC 5 cut(s) 138, 553, 1112, 1408, 1504
BstXI CCANNNNNNTGG 1 cut(s) 1379
BstZI CGGCCG 1 cut(s) 43
BsuRI GGCC 3 cut(s) 45, 143, 998
BtgI CCRYGG 4 cut(s) 40, 190, 560, 1121
BtgZI GCGATG 1 cut(s) 335
BtsCI GGATG 2 cut(s) 1059, 1530
BtsIMutI CAGTG 1 cut(s) 1499
Cac8I GCNNGC 2 cut(s) 327, 974
CfoI GCGC 2 cut(s) 274, 972
Cfr10I RCCGGY 1 cut(s) 1409
Cfr13I GGNCC 3 cut(s) 782, 996, 1238
Cfr42I CCGCGG 2 cut(s) 43, 193
Csp6I GTAC 4 cut(s) 849, 1188, 1375, 1413
CviQI GTAC 4 cut(s) 849, 1188, 1375, 1413
DdeI CTNAG 7 cut(s) 155, 234, 267, 672, 867, 939, 1091
DinI GGCGCC 1 cut(s) 273
DpnI GATC 5 cut(s) 685, 956, 1214, 1226, 1364
DpnII GATC 5 cut(s) 683, 954, 1212, 1224, 1362
DraIII CACNNNGTG 1 cut(s) 770
EaeI YGGCCR 2 cut(s) 43, 141
EagI CGGCCG 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 639
EarI CTCTTC 1 cut(s) 639
Ecl136II GAGCTC 1 cut(s) 605
EclXI CGGCCG 1 cut(s) 43
Eco130I CCWWGG 3 cut(s) 610, 1121, 1477
Eco24I GRGCYC 2 cut(s) 413, 607
Eco32I GATATC 1 cut(s) 873
Eco47I GGWCC 2 cut(s) 782, 1238
Eco52I CGGCCG 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 605
Eco57I CTGAAG 1 cut(s) 1340
EcoICRI GAGCTC 1 cut(s) 605
EcoO109I RGGNCCY 1 cut(s) 1238
EcoRII CCWGG 4 cut(s) 686, 992, 1049, 1240
EcoRV GATATC 1 cut(s) 873
EcoT14I CCWWGG 3 cut(s) 610, 1121, 1477
EcoT22I ATGCAT 1 cut(s) 1023
EcoT38I GRGCYC 2 cut(s) 413, 607
EgeI GGCGCC 1 cut(s) 273
EheI GGCGCC 1 cut(s) 273
ErhI CCWWGG 3 cut(s) 610, 1121, 1477
Esp3I CGTCTC 1 cut(s) 199
FalI AAGNNNNNCTT 2 cut(s) 1183, 1215
FaqI GGGAC 1 cut(s) 493
FauI CCCGC 1 cut(s) 47
Fnu4HI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
FokI GGATG 2 cut(s) 1066, 1517
FriOI GRGCYC 2 cut(s) 413, 607
Fsp4HI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
GlaI GCGC 2 cut(s) 273, 971
GluI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
GsaI CCCAGC 1 cut(s) 1030
GsuI CTGGAG 2 cut(s) 495, 1560
HaeII RGCGCY 2 cut(s) 275, 973
HaeIII GGCC 3 cut(s) 45, 143, 998
HapII CCGG 5 cut(s) 46, 62, 169, 1410, 1545
HhaI GCGC 2 cut(s) 274, 972
Hin1I GRCGYC 1 cut(s) 272
Hin6I GCGC 2 cut(s) 272, 970
HinP1I GCGC 2 cut(s) 272, 970
HincII GTYRAC 2 cut(s) 100, 1087
HindII GTYRAC 2 cut(s) 100, 1087
HinfI GANTC 9 cut(s) 65, 165, 176, 306, 481, 738, 754, 1204, 1351
HpaII CCGG 5 cut(s) 46, 62, 169, 1410, 1545
Hpy166II GTNNAC 4 cut(s) 100, 490, 1087, 1494
Hpy188I TCNGA 7 cut(s) 128, 253, 301, 468, 753, 870, 1168
Hpy188III TCNNGA 4 cut(s) 204, 807, 854, 1358
Hpy8I GTNNAC 4 cut(s) 100, 490, 1087, 1494
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 2 cut(s) 559, 617
HpyCH4III ACNGT 7 cut(s) 113, 122, 359, 561, 1129, 1438, 1503
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 9 cut(s) 117, 348, 642, 704, 839, 1010, 1021, 1219, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 391, 602
HpyF3I CTNAG 7 cut(s) 155, 234, 267, 672, 867, 939, 1091
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 272
HspAI GCGC 2 cut(s) 272, 970
KasI GGCGCC 1 cut(s) 271
KspI CCGCGG 2 cut(s) 43, 193
Kzo9I GATC 5 cut(s) 683, 954, 1212, 1224, 1362
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 3 cut(s) 408, 1372, 1442
Lsp1109I GCAGC 5 cut(s) 138, 553, 1112, 1408, 1504
LweI GCATC 3 cut(s) 312, 1044, 1298
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 762, 1195, 1331
MalI GATC 5 cut(s) 685, 956, 1214, 1226, 1364
MboI GATC 5 cut(s) 683, 954, 1212, 1224, 1362
MboII GAAGA 3 cut(s) 213, 626, 1346
MhlI GDGCHC 3 cut(s) 387, 413, 607
MluCI AATT 6 cut(s) 51, 145, 159, 699, 884, 1460
Mly113I GGCGCC 1 cut(s) 272
MlyI GAGTC 6 cut(s) 159, 170, 315, 475, 732, 1198
MmeI TCCRAC 6 cut(s) 103, 386, 676, 1146, 1294, 1420
MnlI CCTC 9 cut(s) 86, 96, 99, 197, 470, 602, 876, 900, 969
Mph1103I ATGCAT 1 cut(s) 1023
MroXI GAANNNNTTC 1 cut(s) 133
MseI TTAA 3 cut(s) 1001, 1262, 1320
MspA1I CMGCKG 2 cut(s) 42, 192
MspI CCGG 5 cut(s) 46, 62, 169, 1410, 1545
MspR9I CCNGG 5 cut(s) 688, 994, 1051, 1242, 1546
MvaI CCWGG 4 cut(s) 688, 994, 1051, 1242
MvnI CGCG 2 cut(s) 42, 192
MwoI GCNNNNNNNGC 3 cut(s) 326, 391, 602
NarI GGCGCC 1 cut(s) 272
NciI CCSGG 1 cut(s) 1546
NcoI CCATGG 1 cut(s) 1121
NdeII GATC 5 cut(s) 683, 954, 1212, 1224, 1362
NlaIV GGNNCC 2 cut(s) 273, 410
NmeAIII GCCGAG 3 cut(s) 96, 158, 300
NmuCI GTSAC 3 cut(s) 762, 1195, 1331
NsiI ATGCAT 1 cut(s) 1023
PciSI GCTCTTC 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 3 cut(s) 65, 754, 1351
PfoI TCCNGGA 1 cut(s) 1049
PkrI GCNGC 6 cut(s) 44, 153, 568, 1102, 1423, 1519
Ple19I CGATCG 1 cut(s) 1227
PleI GAGTC 6 cut(s) 159, 170, 314, 475, 732, 1198
PluTI GGCGCC 1 cut(s) 275
PpsI GAGTC 6 cut(s) 159, 170, 314, 475, 732, 1198
PpuMI RGGWCCY 1 cut(s) 1238
PshAI GACNNNNGTC 1 cut(s) 861
Psp124BI GAGCTC 1 cut(s) 607
Psp1406I AACGTT 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 1238
Psp6I CCWGG 4 cut(s) 686, 992, 1049, 1240
PspFI CCCAGC 1 cut(s) 1026
PspGI CCWGG 4 cut(s) 686, 992, 1049, 1240
PspN4I GGNNCC 2 cut(s) 273, 410
PspPI GGNCC 3 cut(s) 782, 996, 1238
PspPPI RGGWCCY 1 cut(s) 1238
PvuI CGATCG 1 cut(s) 1227
RsaI GTAC 4 cut(s) 850, 1189, 1376, 1414
RsaNI GTAC 4 cut(s) 849, 1188, 1375, 1413
SacI GAGCTC 1 cut(s) 607
SacII CCGCGG 2 cut(s) 43, 193
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 3 cut(s) 1001, 1262, 1320
SatI GCNGC 6 cut(s) 43, 152, 567, 1101, 1422, 1518
Sau3AI GATC 5 cut(s) 683, 954, 1212, 1224, 1362
Sau96I GGNCC 3 cut(s) 782, 996, 1238
ScaI AGTACT 2 cut(s) 850, 1376
SchI GAGTC 6 cut(s) 159, 170, 315, 475, 732, 1198
ScrFI CCNGG 5 cut(s) 688, 994, 1051, 1242, 1546
SduI GDGCHC 3 cut(s) 387, 413, 607
SfaNI GCATC 3 cut(s) 312, 1044, 1298
SfcI CTRYAG 1 cut(s) 1266
SfoI GGCGCC 1 cut(s) 273
Sfr303I CCGCGG 2 cut(s) 43, 193
SgrBI CCGCGG 2 cut(s) 43, 193
SinI GGWCC 2 cut(s) 782, 1238
Sse9I AATT 6 cut(s) 51, 145, 159, 699, 884, 1460
SsiI CCGC 8 cut(s) 16, 40, 42, 190, 192, 620, 859, 986
SspDI GGCGCC 1 cut(s) 271
SstI GAGCTC 1 cut(s) 607
StyD4I CCNGG 5 cut(s) 686, 992, 1049, 1240, 1544
StyI CCWWGG 3 cut(s) 610, 1121, 1477
TaaI ACNGT 7 cut(s) 113, 122, 359, 561, 1129, 1438, 1503
TaiI ACGT 1 cut(s) 135
TaqI TCGA 4 cut(s) 203, 1207, 1223, 1458
TasI AATT 6 cut(s) 51, 145, 159, 699, 884, 1460
TatI WGTACW 2 cut(s) 848, 1374
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 3 cut(s) 65, 754, 1351
Tru1I TTAA 3 cut(s) 1001, 1262, 1320
Tru9I TTAA 3 cut(s) 1001, 1262, 1320
TscAI CASTG 1 cut(s) 1506
TseFI GTSAC 3 cut(s) 762, 1195, 1331
TseI GCWGC 5 cut(s) 151, 566, 1100, 1421, 1517
Tsp45I GTSAC 3 cut(s) 762, 1195, 1331
TspDTI ATGAA 5 cut(s) 17, 659, 897, 1222, 1581
TspGWI ACGGA 2 cut(s) 85, 1063
TspRI CASTG 1 cut(s) 1506
VpaK11BI GGWCC 2 cut(s) 782, 1238
XapI RAATTY 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 133
ZrmI AGTACT 2 cut(s) 850, 1376
Zsp2I ATGCAT 1 cut(s) 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.