AT3G47400

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016037
Physical Location & Seq
Forward (+)
17465510 .. 17468118
2609 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G47400.1

Sequence Viewer

Length: 1785 bp
ATGCTTAGAGGCATCTTTCACATTTGCCTCTTGGCCTCATTTCTTCTTCTACCGTTCTCCTCCGCCGTACATGATAGTGGCTTCACCGGTGGTACCGATGCTCCTCCTCCGTGGGACCATAATGTTTCCCCACCACCAGAAACCGCACCATCCCCAACCCCCACCTCGAGTCCCTCTACAACCTCACCGCCATCTCCTGGACCGGTGGCTGCGCCATCTCCGATCAACAACGGCTCTGTCTCCGGGGACATGACATGGTGGTGCAACAAGACTCCACACGCTGAGACATGCAATTACTACTTCCGGAAGAGCTCACAAAACAACATTAACCTACGACCACCAAGGTTCCGGTCCGAGTTCTTACGGATGCTGGTCAAGGTAGCACTTGACCAAGCCGTGATCACACACAGTCAAACCGTTAAGTTTGGACCGAGCTGCACCAACAACCAAAGGAAAGCCGCTTGGTCAGACTGCGTAAATCTCTTCCAAAACACGGTGGCTCAGCTGAACCGAACGCTCAAGGGACTTAACCCGGCGGCTTCGAGTGATGTCAAATGTACGGACTTCGACGCACAAACGTGGCTCAGCACGGCTCAGACCAACATCGAGACGTGCCGTTCCGGATCCGAGGATCTTAACGTCTCGGATTTCGTCATGCCGGTGATATCCAACAAGAACTTGTCGGATTTGATTGGAAACTGCTTGGCCGTCAATGGAGTTCTCATGAAGCAACATGATCATACGACTACTGCTAACCACAAAGAATACTTCCCAAGCTGGGTTTCAAGACATGAGAGACGATTACTCGTATCAGCCTCTTTAGCGAAATCGAGTCCCCACCTAGTGGTGGCACAAGACAGGTCCGGCCATTTCCGGTCGATCCAAGCTGCCATAAACTTCGCAGCGAGAAGGAGATTCAAGTCAAGATTTGTGATATATGTGAAGAAAGGTGTTTACCGAGAGAACATTGATGTCGGCAATGATAACCACAACATCATGCTCGTCGGAGATGGCGAGAGAAAAACAATCATCACAAGTGGCCGAAGCGTCCAGCATGGCTACACCACCTATAACTCTGCAACCGGCGGCTTTGGAGGCCAAAGGTTTGTAGCAAAAGACATGACATTCATCAACACGGCCGGGCCATTACGTGGGCAAGCTGTTGCGGTTCGATCATCCTCTGACCTCTCTGTTTTCTACCGTGTCGGTATCCATGGATTCCAAGACACCCTCTACATCCATTCACAACGCCAGTTCTTTAGGGAATGTTATATCTCAGGCACCATCGACTTCATCTTCGGAAATGCAGCTGTTGTCTTCCAGAACTGCATGATTCTCGTCCGGCGCCCTCTTCATGGCCAAGCCAACATCATAACAGCTCAAGGTCGTGGTGACCCTTTTCAGAACACAGGAATAACAATCCATAGCTCAAGGATAATTGCAGCTTCTGATTTGAAGCCTGTGATCCGAGCTTACAAGACCTATTTAGGCCGACCGTGGCAAGCTTATTCACGCGTCACAATCATGAAGACCTATATAGATAACTCGATCAGCCCCTTGGGATGGTCTCCTTGGTTAAGAGGATCCAATTTTGCTCTTAATACAGTTTTCTATGGAGAGTATAAAAATTTTGGACCGGGGTCTTCCACAAGATGGCGGGTCCGGTGGAAAGGATTCCACGCCATAACGAGCACCGCGGTTGCTTCTCGGTTTACGGTTGGAAGCCTTATAGCCGGCGGTTCATGGTTGCCAGCCACTGGCGTGCCTTTCAAGTCAGGGTTGTGA

Protein Analysis

594

Amino Acids

65.49

Weight (kDa)

9.86

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 85 - 237 2e-27 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 281 - 579 2.9e-126 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47400 AT5G51490 AT5G51500
fragaria_vesca FvH4_7g27770
malus_domestica MD11G1195100.v1.1 MD11G1195400.v1.1
prunus_persica Prupe.2G278300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0374561
rosa_laevigata RLG00000026728
rosa_roxburghii Rroxscaffold_4G00283400
rosa_samantha Rh1AG394900 Rh1BG359600 Rh1CG372700 Rh1DG390200
rosa_wichuraiana Rw1G035020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 92
AccB1I GGYRCC 3 cut(s) 92, 1280, 1344
AccB7I CCANNNNNTGG 5 cut(s) 197, 844, 1151, 1653, 1757
AccII CGCG 2 cut(s) 1515, 1697
AccIII TCCGGA 2 cut(s) 303, 620
AclWI GGATC 7 cut(s) 618, 631, 639, 874, 1459, 1578, 1591
AcoI YGGCCR 5 cut(s) 705, 865, 1039, 1137, 1357
AcsI RAATTY 1 cut(s) 1627
AcyI GRCGYC 1 cut(s) 1345
AdeI CACNNNGTG 1 cut(s) 844
AfaI GTAC 3 cut(s) 69, 94, 559
AflIII ACRYGT 1 cut(s) 1513
AgeI ACCGGT 2 cut(s) 86, 202
AgsI TTSAA 4 cut(s) 786, 919, 1456, 1771
AjiI CACGTC 1 cut(s) 612
AjnI CCWGG 1 cut(s) 196
AjuI GAANNNNNNNTTGG 2 cut(s) 766, 798
AleI CACNNNNGTG 2 cut(s) 577, 1760
Alw21I GWGCWC 2 cut(s) 314, 1694
Alw26I GTCTC 6 cut(s) 244, 278, 602, 646, 790, 1572
AlwI GGATC 7 cut(s) 618, 631, 639, 874, 1459, 1578, 1591
AlwNI CAGNNNCTG 2 cut(s) 1448, 1757
Ama87I CYCGRG 1 cut(s) 166
Aor13HI TCCGGA 2 cut(s) 303, 620
AoxI GGCC 9 cut(s) 33, 705, 865, 1039, 1096, 1137, 1142, 1357, 1489
ApeKI GCWGC 6 cut(s) 209, 435, 887, 902, 1307, 1442
ApoI RAATTY 1 cut(s) 1627
ArsI GACNNNNNNTTYG 2 cut(s) 1280, 1312
AsiGI ACCGGT 2 cut(s) 86, 202
Asp700I GAANNNNTTC 1 cut(s) 1673
Asp718I GGTACC 1 cut(s) 92
AspLEI GCGC 2 cut(s) 214, 1347
AspS9I GGNCC 8 cut(s) 115, 200, 351, 428, 861, 1142, 1634, 1660
AsuC2I CCSGG 4 cut(s) 244, 533, 1141, 1638
AsuHPI GGTGA 4 cut(s) 76, 177, 673, 1403
AvaI CYCGRG 1 cut(s) 166
AvaII GGWCC 7 cut(s) 115, 200, 351, 428, 861, 1634, 1660
BalI TGGCCA 1 cut(s) 1359
BamHI GGATCC 2 cut(s) 623, 1583
BanI GGYRCC 3 cut(s) 92, 1280, 1344
BanII GRGCYC 1 cut(s) 314
BbsI GAAGAC 3 cut(s) 1309, 1535, 1635
Bbv12I GWGCWC 2 cut(s) 314, 1694
BbvI GCAGC 6 cut(s) 196, 422, 874, 914, 1319, 1454
BccI CCATC 7 cut(s) 157, 199, 223, 1004, 1292, 1557, 1647
BceAI ACGGC 7 cut(s) 50, 247, 380, 600, 606, 692, 1152
BcgI CGANNNNNNTGC 2 cut(s) 1318, 1352
BciT130I CCWGG 1 cut(s) 198
BciVI GTATCC 1 cut(s) 1220
BclI TGATCA 2 cut(s) 399, 736
BcnI CCSGG 4 cut(s) 244, 533, 1141, 1638
BcoDI GTCTC 6 cut(s) 244, 278, 602, 646, 790, 1572
BfaI CTAG 1 cut(s) 842
BfoI RGCGCY 1 cut(s) 1348
BfuI GTATCC 1 cut(s) 1220
BisI GCNGC 9 cut(s) 210, 436, 459, 537, 888, 903, 1087, 1308, 1443
BlpI GCTNAGC 2 cut(s) 501, 584
BlsI GCNGC 9 cut(s) 211, 437, 460, 538, 889, 904, 1088, 1309, 1444
Bme1390I CCNGG 5 cut(s) 198, 244, 533, 1141, 1638
Bme18I GGWCC 7 cut(s) 115, 200, 351, 428, 861, 1634, 1660
BmeT110I CYCGRG 1 cut(s) 166
BmgBI CACGTC 1 cut(s) 612
BmgT120I GGNCC 8 cut(s) 115, 200, 351, 428, 861, 1142, 1634, 1660
BmiI GGNNCC 8 cut(s) 94, 116, 347, 625, 1282, 1346, 1585, 1661
BmrFI CCNGG 5 cut(s) 198, 244, 533, 1141, 1638
BmsI GCATC 3 cut(s) 21, 88, 357
BoxI GACNNNNGTC 1 cut(s) 1639
BpiI GAAGAC 3 cut(s) 1309, 1535, 1635
Bpu1102I GCTNAGC 2 cut(s) 501, 584
BpuEI CTTGAG 3 cut(s) 503, 1365, 1414
BpuMI CCSGG 4 cut(s) 244, 533, 1141, 1638
BsaAI YACGTR 1 cut(s) 1151
BsaHI GRCGYC 1 cut(s) 1345
BsaI GGTCTC 1 cut(s) 1572
BsaWI WCCGGW 7 cut(s) 86, 202, 303, 348, 620, 873, 1662
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bse118I RCCGGY 5 cut(s) 86, 202, 658, 1082, 1733
Bse1I ACTGG 2 cut(s) 1252, 1762
Bse3DI GCAATG 1 cut(s) 985
BseAI TCCGGA 2 cut(s) 303, 620
BseBI CCWGG 1 cut(s) 198
BseGI GGATG 5 cut(s) 149, 372, 1175, 1236, 1568
BseMI GCAATG 1 cut(s) 985
BseMII CTCAG 5 cut(s) 273, 515, 598, 608, 1290
BseNI ACTGG 2 cut(s) 1252, 1762
BseRI GAGGAG 3 cut(s) 49, 93, 96
BseX3I CGGCCG 1 cut(s) 1137
BseXI GCAGC 6 cut(s) 196, 422, 874, 914, 1319, 1454
BseYI CCCAGC 1 cut(s) 777
BsgI GTGCAG 1 cut(s) 421
Bsh1236I CGCG 2 cut(s) 1515, 1697
Bsh1285I CGRYCG 3 cut(s) 878, 1140, 1496
BshFI GGCC 9 cut(s) 35, 707, 867, 1041, 1098, 1139, 1144, 1359, 1491
BshNI GGYRCC 3 cut(s) 92, 1280, 1344
BshTI ACCGGT 2 cut(s) 86, 202
BsiEI CGRYCG 3 cut(s) 878, 1140, 1496
BsiHKAI GWGCWC 2 cut(s) 314, 1694
BsiHKCI CYCGRG 1 cut(s) 166
BslFI GGGAC 5 cut(s) 128, 156, 260, 537, 819
BsmAI GTCTC 6 cut(s) 244, 278, 602, 646, 790, 1572
BsmBI CGTCTC 3 cut(s) 602, 646, 790
BsmFI GGGAC 5 cut(s) 128, 156, 260, 537, 819
BsnI GGCC 9 cut(s) 35, 707, 867, 1041, 1098, 1139, 1144, 1359, 1491
Bso31I GGTCTC 1 cut(s) 1572
BsoBI CYCGRG 1 cut(s) 166
Bsp1286I GDGCHC 2 cut(s) 314, 1694
Bsp13I TCCGGA 2 cut(s) 303, 620
Bsp1720I GCTNAGC 2 cut(s) 501, 584
Bsp19I CCATGG 1 cut(s) 1213
BspANI GGCC 9 cut(s) 35, 707, 867, 1041, 1098, 1139, 1144, 1359, 1491
BspCNI CTCAG 5 cut(s) 274, 514, 597, 607, 1289
BspEI TCCGGA 2 cut(s) 303, 620
BspFNI CGCG 2 cut(s) 1515, 1697
BspHI TCATGA 2 cut(s) 723, 1524
BspLI GGNNCC 8 cut(s) 94, 116, 347, 625, 1282, 1346, 1585, 1661
BspPI GGATC 7 cut(s) 618, 631, 639, 874, 1459, 1578, 1591
BspQI GCTCTTC 1 cut(s) 302
BspT107I GGYRCC 3 cut(s) 92, 1280, 1344
BspTNI GGTCTC 1 cut(s) 1572
BsrDI GCAATG 1 cut(s) 985
BsrFI RCCGGY 5 cut(s) 86, 202, 658, 1082, 1733
BsrI ACTGG 2 cut(s) 1252, 1762
BssAI RCCGGY 5 cut(s) 86, 202, 658, 1082, 1733
BssNI GRCGYC 1 cut(s) 1345
BssT1I CCWWGG 4 cut(s) 341, 1213, 1557, 1571
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 8 cut(s) 54, 410, 418, 496, 1202, 1497, 1606, 1717
Bst6I CTCTTC 3 cut(s) 302, 488, 1356
BstACI GRCGYC 1 cut(s) 1345
BstBAI YACGTR 1 cut(s) 1151
BstC8I GCNNGC 5 cut(s) 1158, 1503, 1735, 1752, 1763
BstDEI CTNAG 6 cut(s) 5, 282, 501, 584, 594, 1276
BstDSI CCRYGG 4 cut(s) 110, 1213, 1496, 1695
BstEII GGTNACC 1 cut(s) 1391
BstF5I GGATG 5 cut(s) 149, 372, 1175, 1236, 1568
BstFNI CGCG 2 cut(s) 1515, 1697
BstH2I RGCGCY 1 cut(s) 1348
BstHHI GCGC 2 cut(s) 214, 1347
BstMAI GTCTC 6 cut(s) 244, 278, 602, 646, 790, 1572
BstMCI CGRYCG 3 cut(s) 878, 1140, 1496
BstMWI GCNNNNNNNGC 2 cut(s) 821, 1095
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 291
BstPAI GACNNNNGTC 1 cut(s) 1639
BstPI GGTNACC 1 cut(s) 1391
BstSCI CCNGG 5 cut(s) 196, 242, 531, 1139, 1636
BstUI CGCG 2 cut(s) 1515, 1697
BstV1I GCAGC 6 cut(s) 196, 422, 874, 914, 1319, 1454
BstV2I GAAGAC 3 cut(s) 1309, 1535, 1635
BstX2I RGATCY 3 cut(s) 623, 631, 1583
BstYI RGATCY 3 cut(s) 623, 631, 1583
BstZI CGGCCG 1 cut(s) 1137
BsuI GTATCC 1 cut(s) 1220
BsuRI GGCC 9 cut(s) 35, 707, 867, 1041, 1098, 1139, 1144, 1359, 1491
BtgI CCRYGG 4 cut(s) 110, 1213, 1496, 1695
BtrI CACGTC 1 cut(s) 612
BtsCI GGATG 5 cut(s) 149, 372, 1175, 1236, 1568
BtsIMutI CAGTG 1 cut(s) 1755
Cac8I GCNNGC 5 cut(s) 1158, 1503, 1735, 1752, 1763
CaiI CAGNNNCTG 2 cut(s) 1448, 1757
CciI TCATGA 2 cut(s) 723, 1524
CfoI GCGC 2 cut(s) 214, 1347
Cfr10I RCCGGY 5 cut(s) 86, 202, 658, 1082, 1733
Cfr13I GGNCC 8 cut(s) 115, 200, 351, 428, 861, 1142, 1634, 1660
Cfr42I CCGCGG 1 cut(s) 1698
CpoI CGGWCCG 1 cut(s) 351
CseI GACGC 3 cut(s) 578, 1036, 1504
Csp6I GTAC 3 cut(s) 68, 93, 558
CspAI ACCGGT 2 cut(s) 86, 202
CspI CGGWCCG 1 cut(s) 351
CviQI GTAC 3 cut(s) 68, 93, 558
DdeI CTNAG 6 cut(s) 5, 282, 501, 584, 594, 1276
DinI GGCGCC 1 cut(s) 1346
DraIII CACNNNGTG 1 cut(s) 844
EaeI YGGCCR 5 cut(s) 705, 865, 1039, 1137, 1357
EagI CGGCCG 1 cut(s) 1137
Eam1104I CTCTTC 3 cut(s) 302, 488, 1356
EarI CTCTTC 3 cut(s) 302, 488, 1356
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 312
EclXI CGGCCG 1 cut(s) 1137
Eco130I CCWWGG 4 cut(s) 341, 1213, 1557, 1571
Eco24I GRGCYC 1 cut(s) 314
Eco31I GGTCTC 1 cut(s) 1572
Eco32I GATATC 1 cut(s) 666
Eco47I GGWCC 7 cut(s) 115, 200, 351, 428, 861, 1634, 1660
Eco52I CGGCCG 1 cut(s) 1137
Eco53kI GAGCTC 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 166
Eco91I GGTNACC 1 cut(s) 1391
EcoICRI GAGCTC 1 cut(s) 312
EcoO65I GGTNACC 1 cut(s) 1391
EcoRII CCWGG 1 cut(s) 196
EcoRV GATATC 1 cut(s) 666
EcoT14I CCWWGG 4 cut(s) 341, 1213, 1557, 1571
EcoT38I GRGCYC 1 cut(s) 314
EgeI GGCGCC 1 cut(s) 1346
EheI GGCGCC 1 cut(s) 1346
ErhI CCWWGG 4 cut(s) 341, 1213, 1557, 1571
Esp3I CGTCTC 3 cut(s) 602, 646, 790
FaqI GGGAC 5 cut(s) 128, 156, 260, 537, 819
FauI CCCGC 1 cut(s) 1650
FbaI TGATCA 2 cut(s) 399, 736
Fnu4HI GCNGC 9 cut(s) 210, 436, 459, 537, 888, 903, 1087, 1308, 1443
FokI GGATG 5 cut(s) 136, 379, 1162, 1223, 1575
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 9 cut(s) 210, 436, 459, 537, 888, 903, 1087, 1308, 1443
FspBI CTAG 1 cut(s) 842
GlaI GCGC 2 cut(s) 213, 1346
GluI GCNGC 9 cut(s) 210, 436, 459, 537, 888, 903, 1087, 1308, 1443
GsaI CCCAGC 1 cut(s) 781
HaeII RGCGCY 1 cut(s) 1348
HaeIII GGCC 9 cut(s) 35, 707, 867, 1041, 1098, 1139, 1144, 1359, 1491
HgaI GACGC 3 cut(s) 578, 1036, 1504
HhaI GCGC 2 cut(s) 214, 1347
Hin1I GRCGYC 1 cut(s) 1345
Hin6I GCGC 2 cut(s) 212, 1345
HinP1I GCGC 2 cut(s) 212, 1345
HindIII AAGCTT 1 cut(s) 1503
HinfI GANTC 7 cut(s) 169, 271, 832, 915, 1218, 1333, 1674
HphI GGTGA 4 cut(s) 76, 177, 673, 1403
Hpy166II GTNNAC 2 cut(s) 955, 1713
Hpy188III TCNNGA 8 cut(s) 304, 607, 621, 724, 786, 924, 1321, 1525
Hpy8I GTNNAC 2 cut(s) 955, 1713
Hpy99I CGWCG 2 cut(s) 572, 1007
HpyAV CCTTC 1 cut(s) 903
HpyCH4III ACNGT 8 cut(s) 54, 410, 418, 496, 1202, 1497, 1606, 1717
HpyCH4IV ACGT 4 cut(s) 578, 611, 639, 1150
HpyCH4V TGCA 7 cut(s) 264, 291, 438, 1079, 1307, 1329, 1442
HpyF10VI GCNNNNNNNGC 2 cut(s) 821, 1095
HpyF3I CTNAG 6 cut(s) 5, 282, 501, 584, 594, 1276
HpySE526I ACGT 4 cut(s) 578, 611, 639, 1150
Hsp92I GRCGYC 1 cut(s) 1345
HspAI GCGC 2 cut(s) 212, 1345
KasI GGCGCC 1 cut(s) 1344
Kpn2I TCCGGA 2 cut(s) 303, 620
KpnI GGTACC 1 cut(s) 96
KroI GCCGGC 1 cut(s) 1733
KroNI GCCGGC 1 cut(s) 1735
Ksp22I TGATCA 2 cut(s) 399, 736
KspI CCGCGG 1 cut(s) 1698
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 1 cut(s) 106
Lsp1109I GCAGC 6 cut(s) 196, 422, 874, 914, 1319, 1454
LweI GCATC 3 cut(s) 21, 88, 357
MaeI CTAG 1 cut(s) 842
MaeII ACGT 4 cut(s) 578, 611, 639, 1150
MaeIII GTNAC 2 cut(s) 1391, 1516
MflI RGATCY 3 cut(s) 623, 631, 1583
MhlI GDGCHC 2 cut(s) 314, 1694
MlsI TGGCCA 1 cut(s) 1359
MluCI AATT 4 cut(s) 292, 1437, 1588, 1627
MluI ACGCGT 1 cut(s) 1513
MluNI TGGCCA 1 cut(s) 1359
Mly113I GGCGCC 1 cut(s) 1345
MlyI GAGTC 3 cut(s) 178, 265, 841
MmeI TCCRAC 4 cut(s) 663, 693, 985, 1699
Mox20I TGGCCA 1 cut(s) 1359
MroI TCCGGA 2 cut(s) 303, 620
MroNI GCCGGC 1 cut(s) 1733
MroXI GAANNNNTTC 1 cut(s) 1673
MscI TGGCCA 1 cut(s) 1359
MseI TTAA 6 cut(s) 327, 420, 528, 636, 1577, 1599
MslI CAYNNNNRTG 6 cut(s) 75, 259, 577, 659, 1523, 1760
Msp20I TGGCCA 1 cut(s) 1359
MspA1I CMGCKG 3 cut(s) 505, 1310, 1697
MspR9I CCNGG 5 cut(s) 198, 244, 533, 1141, 1638
MvaI CCWGG 1 cut(s) 198
MvnI CGCG 2 cut(s) 1515, 1697
MwoI GCNNNNNNNGC 2 cut(s) 821, 1095
NaeI GCCGGC 1 cut(s) 1735
NarI GGCGCC 1 cut(s) 1345
NciI CCSGG 4 cut(s) 244, 533, 1141, 1638
NcoI CCATGG 1 cut(s) 1213
NgoMIV GCCGGC 1 cut(s) 1733
NlaIV GGNNCC 8 cut(s) 94, 116, 347, 625, 1282, 1346, 1585, 1661
NmuCI GTSAC 2 cut(s) 1391, 1516
NspI RCATGY 1 cut(s) 291
OliI CACNNNNGTG 2 cut(s) 577, 1760
PaeR7I CTCGAG 1 cut(s) 166
PagI TCATGA 2 cut(s) 723, 1524
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 1011
PdiI GCCGGC 1 cut(s) 1735
PdmI GAANNNNTTC 1 cut(s) 1673
PfeI GAWTC 4 cut(s) 915, 1218, 1333, 1674
PflMI CCANNNNNTGG 5 cut(s) 197, 844, 1151, 1653, 1757
PfoI TCCNGGA 1 cut(s) 196
PinAI ACCGGT 2 cut(s) 86, 202
PkrI GCNGC 9 cut(s) 211, 437, 460, 538, 889, 904, 1088, 1309, 1444
PleI GAGTC 3 cut(s) 177, 265, 840
PluTI GGCGCC 1 cut(s) 1348
PpsI GAGTC 3 cut(s) 177, 265, 840
Ppu21I YACGTR 1 cut(s) 1151
PshAI GACNNNNGTC 1 cut(s) 1639
Psp124BI GAGCTC 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 196
PspEI GGTNACC 1 cut(s) 1391
PspFI CCCAGC 1 cut(s) 777
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 8 cut(s) 94, 116, 347, 625, 1282, 1346, 1585, 1661
PspPI GGNCC 8 cut(s) 115, 200, 351, 428, 861, 1142, 1634, 1660
PspXI VCTCGAGB 1 cut(s) 166
PstNI CAGNNNCTG 2 cut(s) 1448, 1757
PsuI RGATCY 3 cut(s) 623, 631, 1583
PvuII CAGCTG 2 cut(s) 505, 1310
RsaI GTAC 3 cut(s) 69, 94, 559
RsaNI GTAC 3 cut(s) 68, 93, 558
RseI CAYNNNNRTG 6 cut(s) 75, 259, 577, 659, 1523, 1760
Rsr2I CGGWCCG 1 cut(s) 351
RsrII CGGWCCG 1 cut(s) 351
SacI GAGCTC 1 cut(s) 314
SacII CCGCGG 1 cut(s) 1698
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 6 cut(s) 327, 420, 528, 636, 1577, 1599
SatI GCNGC 9 cut(s) 210, 436, 459, 537, 888, 903, 1087, 1308, 1443
Sau96I GGNCC 8 cut(s) 115, 200, 351, 428, 861, 1142, 1634, 1660
SchI GAGTC 3 cut(s) 178, 265, 841
ScrFI CCNGG 5 cut(s) 198, 244, 533, 1141, 1638
SduI GDGCHC 2 cut(s) 314, 1694
SfaNI GCATC 3 cut(s) 21, 88, 357
SfoI GGCGCC 1 cut(s) 1346
Sfr274I CTCGAG 1 cut(s) 166
Sfr303I CCGCGG 1 cut(s) 1698
SgrAI CRCCGGYG 1 cut(s) 86
SgrBI CCGCGG 1 cut(s) 1698
SinI GGWCC 7 cut(s) 115, 200, 351, 428, 861, 1634, 1660
SlaI CTCGAG 1 cut(s) 166
SmiMI CAYNNNNRTG 6 cut(s) 75, 259, 577, 659, 1523, 1760
SmlI CTYRAG 4 cut(s) 166, 518, 1380, 1429
SmoI CTYRAG 4 cut(s) 166, 518, 1380, 1429
Sse9I AATT 4 cut(s) 292, 1437, 1588, 1627
SspDI GGCGCC 1 cut(s) 1344
SspMI CTAG 1 cut(s) 842
SstI GAGCTC 1 cut(s) 314
StyD4I CCNGG 5 cut(s) 196, 242, 531, 1139, 1636
StyI CCWWGG 4 cut(s) 341, 1213, 1557, 1571
TaaI ACNGT 8 cut(s) 54, 410, 418, 496, 1202, 1497, 1606, 1717
TaiI ACGT 4 cut(s) 581, 614, 642, 1153
TaqI TCGA 9 cut(s) 167, 542, 567, 606, 830, 878, 1171, 1287, 1547
TaqII GACCGA 1 cut(s) 445
TasI AATT 4 cut(s) 292, 1437, 1588, 1627
TauI GCSGC 3 cut(s) 461, 539, 1089
TfiI GAWTC 4 cut(s) 915, 1218, 1333, 1674
Tru1I TTAA 6 cut(s) 327, 420, 528, 636, 1577, 1599
Tru9I TTAA 6 cut(s) 327, 420, 528, 636, 1577, 1599
TscAI CASTG 1 cut(s) 1762
TseFI GTSAC 2 cut(s) 1391, 1516
TseI GCWGC 6 cut(s) 209, 435, 887, 902, 1307, 1442
Tsp45I GTSAC 2 cut(s) 1391, 1516
TspDTI ATGAA 6 cut(s) 740, 1117, 1282, 1343, 1541, 1731
TspGWI ACGGA 3 cut(s) 99, 379, 575
TspRI CASTG 1 cut(s) 1762
Van91I CCANNNNNTGG 5 cut(s) 197, 844, 1151, 1653, 1757
VpaK11BI GGWCC 7 cut(s) 115, 200, 351, 428, 861, 1634, 1660
XapI RAATTY 1 cut(s) 1627
XceI RCATGY 1 cut(s) 291
XhoI CTCGAG 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 1673
XspI CTAG 1 cut(s) 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.