MD11G1195400.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016037
Physical Location & Seq
Forward (+)
28018077 .. 28020238
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1195400.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGCTTCACATTCGCAAATTTGCCCGCTTCTCTTTATTTTTCTTACTCATATTCATCTCTTTGTTCTTCCACACAACCCTAGCCAGAAGTCACCACCGCCACTCTAGTCGTATACGTACGTGGTGCAGAAAAACCCCTCACCCAGAGTCTTGCAACTACTGCTTGTCGAATAGCATCCACCGGTTCCCCCCGAAACACACCTCCGAGTTCCGAAAAATGCTGGTTCAAGTGGCCTTGGAGAGAGCTCTCGATGCCATAACATACACCTTCCAGTATGGCCAAAACTGCCGAAACAATAACGAAAAATCCGCGTGGGCCGATTGCTTGAAACTTTTCGAGAACACCGTCAACCAGCTCAATGTTACACTTGAAGGCTTAGGCAACAAACGCAAGTGTTCTAATTTTGATGCGCAAACTTGGCTCAGCGCTGCCCTCACCAACATTCACACGTGCCGAGTAGGGTCTTTGGAACTGAACGTTACGGACTTCATAACGCCGATTTACAGGTCCAGTAACAATGTGTCGGAGCTCATTAGCAACAGTTTAGCAATAGGTTCGCAACTTTTAGGCACGGAAGAAAGTTACAATGAGAGCGAAAAGTTTCCAAATTGGGTTTCGAAGCATGACCGGAGGTTATTGCAAGCTTCAAACATTAGGGCAAATCTTGTGGTGGCTAAAGATGGATCGGGGCATTTTTCGACCGTCCAAGCCGCCTTAGATGCAGCCGCGAGGAGAAGAGTCAAGAGCAAGTTTATAATCTATGTGAAGAAAGGTGTCTACCCGGAGAACATTCAAGTTAGCAACACTAACAGTAAAATCATGTTGGTTGGTGCTGGCATTAGATATACAATAATCACATCTAGCCGAAGTGTCAGTGAAGGTTACACAACCTATAATTCTGCAACTGCTGGCATAAACGGTCATGGATTCATTGCTCGTGGCATCACATTTAGCAACACTGCCGGACCACTAAAAGGCCAAGCTGTGGCGCTCAGATCGGCATCCGACCTCTCGGTCTTCTACCGATGCGCCTTCCAGGGCTACCAAGACACCCTCATGGTCTACTCCCAGCGCCAGTTCTACAGAGAATGCTACATCTTTGGCACCATAGACTTCATATTTGGTAACGCAGCTGCAGTTTTCCAAAATTGCATGATATATGTGAGAAAGCCATTGTGGGGCCAAGTCAACGTCATCACGGCACAAGGTCGAAACGACCCGTTTCAAAACACAGCAATTTCGATCCACAATTCTCAGGTACGGGCAGCCGCTGACCTAAAGCCCGTGGCCGGTTCTTACAAAACATATTTGGGCCGGCCGTGGATGCAGTACTCTAGGGTTGTCTTTATGAAATGTTATCTGGACAATTTGGTAAATCCGGCGGGCTGGTTGCGGTGGCAAAGAAGTAATTTTGCCCTTAGCACTTTGTATTATGGGGAGTATCAGAACTTTGGACCTGGCTCTTGTACTAGGTATAGAGTGAAGTGGCCTGGTTTTCATGCCATCACCAGTCCAAATGTGGCGTCTATGTTCACCGCCGGTAGCCTTATTGCTGGTTGGTCGTGGTTGCCCACCACCGGTGTGCCGTTCACAATGGGTCTATGA

Protein Analysis

535

Amino Acids

60.27

Weight (kDa)

9.79

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 184 8.9e-30 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 221 - 519 3.1e-127 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47400 AT5G51490 AT5G51500
fragaria_vesca FvH4_7g27770
malus_domestica MD11G1195100.v1.1 MD11G1195400.v1.1
prunus_persica Prupe.2G278300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0374561
rosa_laevigata RLG00000026728
rosa_roxburghii Rroxscaffold_4G00283400
rosa_samantha Rh1AG394900 Rh1BG359600 Rh1CG372700 Rh1DG390200
rosa_wichuraiana Rw1G035020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AasI GACNNNNNNGTC 1 cut(s) 1013
Acc16I TGCGCA 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 1103
AccB7I CCANNNNNTGG 1 cut(s) 985
AccI GTMKAC 3 cut(s) 112, 777, 1062
AccII CGCG 2 cut(s) 311, 728
AciI CCGC 9 cut(s) 25, 97, 309, 711, 726, 1269, 1382, 1393, 1536
AclI AACGTT 1 cut(s) 477
AclWI GGATC 2 cut(s) 691, 1237
AcoI YGGCCR 3 cut(s) 277, 1287, 1316
AcsI RAATTY 1 cut(s) 17
AcvI CACGTG 1 cut(s) 450
AcyI GRCGYC 1 cut(s) 1523
AfaI GTAC 4 cut(s) 118, 1260, 1331, 1468
AfeI AGCGCT 1 cut(s) 427
AfiI CCNNNNNNNGG 5 cut(s) 974, 985, 1178, 1289, 1577
AflIII ACRYGT 1 cut(s) 447
AgeI ACCGGT 2 cut(s) 180, 1577
AgsI TTSAA 6 cut(s) 227, 328, 371, 648, 794, 1226
AjnI CCWGG 3 cut(s) 1035, 1456, 1489
AjuI GAANNNNNNNTTGG 2 cut(s) 598, 630
AleI CACNNNNGTG 1 cut(s) 1580
AluBI AGCT 6 cut(s) 245, 355, 529, 644, 983, 1133
AluI AGCT 6 cut(s) 245, 355, 529, 644, 983, 1133
Alw21I GWGCWC 2 cut(s) 247, 531
AlwI GGATC 2 cut(s) 691, 1237
AlwNI CAGNNNCTG 1 cut(s) 1271
Aor51HI AGCGCT 1 cut(s) 427
AoxI GGCC 9 cut(s) 231, 277, 315, 976, 1180, 1287, 1312, 1316, 1487
ApeKI GCWGC 5 cut(s) 428, 722, 1130, 1133, 1265
ApoI RAATTY 1 cut(s) 17
ArsI GACNNNNNNTTYG 4 cut(s) 1103, 1135, 1508, 1540
AsiGI ACCGGT 2 cut(s) 180, 1577
Asp700I GAANNNNTTC 2 cut(s) 332, 600
AspLEI GCGC 5 cut(s) 412, 428, 991, 1031, 1074
AspS9I GGNCC 6 cut(s) 315, 507, 965, 1180, 1312, 1454
AsuC2I CCSGG 1 cut(s) 782
AsuHPI GGTGA 5 cut(s) 83, 131, 427, 1498, 1525
AsuII TTCGAA 1 cut(s) 617
AvaII GGWCC 3 cut(s) 507, 965, 1454
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 1 cut(s) 1103
BanII GRGCYC 2 cut(s) 247, 531
BauI CACGAG 1 cut(s) 936
BbrPI CACGTG 1 cut(s) 450
BbsI GAAGAC 1 cut(s) 1009
Bbv12I GWGCWC 2 cut(s) 247, 531
BbvI GCAGC 5 cut(s) 415, 734, 1120, 1142, 1277
BccI CCATC 2 cut(s) 674, 1511
BceAI ACGGC 3 cut(s) 1215, 1303, 1570
BcgI CGANNNNNNTGC 2 cut(s) 537, 571
BciT130I CCWGG 3 cut(s) 1037, 1458, 1491
BcnI CCSGG 1 cut(s) 782
BfaI CTAG 5 cut(s) 80, 105, 861, 1335, 1470
BfmI CTRYAG 2 cut(s) 1081, 1134
BfoI RGCGCY 3 cut(s) 429, 992, 1075
BisI GCNGC 8 cut(s) 429, 711, 723, 726, 1131, 1134, 1266, 1269
BlpI GCTNAGC 1 cut(s) 422
BlsI GCNGC 8 cut(s) 430, 712, 724, 727, 1132, 1135, 1267, 1270
BmcAI AGTACT 1 cut(s) 1331
Bme1390I CCNGG 4 cut(s) 782, 1037, 1458, 1491
Bme18I GGWCC 3 cut(s) 507, 965, 1454
BmgT120I GGNCC 6 cut(s) 315, 507, 965, 1180, 1312, 1454
BmiI GGNNCC 3 cut(s) 185, 1105, 1181
BmrFI CCNGG 4 cut(s) 782, 1037, 1458, 1491
BmsI GCATC 8 cut(s) 183, 241, 397, 709, 951, 1010, 1016, 1314
BpiI GAAGAC 1 cut(s) 1009
Bpu10I CCTNAGC 2 cut(s) 376, 1418
Bpu1102I GCTNAGC 1 cut(s) 422
Bpu14I TTCGAA 1 cut(s) 617
BpuMI CCSGG 1 cut(s) 782
BsaAI YACGTR 3 cut(s) 116, 120, 450
BsaBI GATNNNNATC 1 cut(s) 1000
BsaHI GRCGYC 1 cut(s) 1523
BsaJI CCNNGG 4 cut(s) 234, 1036, 1284, 1319
BsaWI WCCGGW 3 cut(s) 180, 627, 1577
Bsc4I CCNNNNNNNGG 5 cut(s) 974, 985, 1178, 1289, 1577
Bse118I RCCGGY 5 cut(s) 180, 1289, 1314, 1538, 1577
Bse1I ACTGG 4 cut(s) 271, 510, 1075, 1509
Bse3DI GCAATG 1 cut(s) 930
Bse8I GATNNNNATC 1 cut(s) 1000
BseBI CCWGG 3 cut(s) 1037, 1458, 1491
BseDI CCNNGG 4 cut(s) 234, 1036, 1284, 1319
BseGI GGATG 3 cut(s) 174, 1001, 1329
BseJI GATNNNNATC 1 cut(s) 1000
BseLI CCNNNNNNNGG 5 cut(s) 974, 985, 1178, 1289, 1577
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 3 cut(s) 436, 1006, 1268
BseNI ACTGG 4 cut(s) 271, 510, 1075, 1509
BseRI GAGGAG 1 cut(s) 745
BseX3I CGGCCG 1 cut(s) 1316
BseXI GCAGC 5 cut(s) 415, 734, 1120, 1142, 1277
BseYI CCCAGC 1 cut(s) 1068
BsgI GTGCAG 1 cut(s) 145
Bsh1236I CGCG 2 cut(s) 311, 728
Bsh1285I CGRYCG 2 cut(s) 702, 1319
BshFI GGCC 9 cut(s) 233, 279, 317, 978, 1182, 1289, 1314, 1318, 1489
BshNI GGYRCC 1 cut(s) 1103
BshTI ACCGGT 2 cut(s) 180, 1577
BsiEI CGRYCG 2 cut(s) 702, 1319
BsiHKAI GWGCWC 2 cut(s) 247, 531
BsiSI CCGG 9 cut(s) 181, 628, 782, 963, 1290, 1315, 1379, 1539, 1578
BsiWI CGTACG 1 cut(s) 116
BslI CCNNNNNNNGG 5 cut(s) 974, 985, 1178, 1289, 1577
BsmI GAATGC 1 cut(s) 1094
BsnI GGCC 9 cut(s) 233, 279, 317, 978, 1182, 1289, 1314, 1318, 1489
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 2 cut(s) 247, 531
Bsp143I GATC 3 cut(s) 683, 995, 1242
Bsp1720I GCTNAGC 1 cut(s) 422
BspACI CCGC 9 cut(s) 25, 97, 309, 711, 726, 1269, 1382, 1393, 1536
BspANI GGCC 9 cut(s) 233, 279, 317, 978, 1182, 1289, 1314, 1318, 1489
BspCNI CTCAG 3 cut(s) 435, 1005, 1267
BspFNI CGCG 2 cut(s) 311, 728
BspLI GGNNCC 3 cut(s) 185, 1105, 1181
BspMAI CTGCAG 1 cut(s) 1138
BspPI GGATC 2 cut(s) 691, 1237
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 1103
BsrDI GCAATG 1 cut(s) 930
BsrFI RCCGGY 5 cut(s) 180, 1289, 1314, 1538, 1577
BsrI ACTGG 4 cut(s) 271, 510, 1075, 1509
BssAI RCCGGY 5 cut(s) 180, 1289, 1314, 1538, 1577
BssECI CCNNGG 4 cut(s) 234, 1036, 1284, 1319
BssMI GATC 3 cut(s) 683, 995, 1242
BssNAI GTATAC 1 cut(s) 113
BssNI GRCGYC 1 cut(s) 1523
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 1 cut(s) 234
Bst1107I GTATAC 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 3 cut(s) 1037, 1458, 1491
Bst4CI ACNGT 5 cut(s) 346, 542, 703, 812, 920
Bst6I CTCTTC 1 cut(s) 730
BstACI GRCGYC 1 cut(s) 1523
BstAPI GCANNNNNTGC 1 cut(s) 159
BstBAI YACGTR 3 cut(s) 116, 120, 450
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 6 cut(s) 25, 642, 835, 910, 1316, 1384
BstDEI CTNAG 6 cut(s) 376, 422, 715, 992, 1254, 1418
BstDSI CCRYGG 2 cut(s) 1284, 1319
BstF5I GGATG 3 cut(s) 174, 1001, 1329
BstFNI CGCG 2 cut(s) 311, 728
BstH2I RGCGCY 3 cut(s) 429, 992, 1075
BstHHI GCGC 5 cut(s) 412, 428, 991, 1031, 1074
BstKTI GATC 3 cut(s) 686, 998, 1245
BstMBI GATC 3 cut(s) 683, 995, 1242
BstMCI CGRYCG 2 cut(s) 702, 1319
BstMWI GCNNNNNNNGC 7 cut(s) 159, 251, 285, 387, 418, 719, 1324
BstNI CCWGG 3 cut(s) 1037, 1458, 1491
BstSCI CCNGG 4 cut(s) 780, 1035, 1456, 1489
BstSFI CTRYAG 2 cut(s) 1081, 1134
BstSNI TACGTA 1 cut(s) 116
BstUI CGCG 2 cut(s) 311, 728
BstV1I GCAGC 5 cut(s) 415, 734, 1120, 1142, 1277
BstV2I GAAGAC 1 cut(s) 1009
BstZ17I GTATAC 1 cut(s) 113
BstZI CGGCCG 1 cut(s) 1316
BsuRI GGCC 9 cut(s) 233, 279, 317, 978, 1182, 1289, 1314, 1318, 1489
BtgI CCRYGG 2 cut(s) 1284, 1319
BtsCI GGATG 3 cut(s) 174, 1001, 1329
BtsI GCAGTG 1 cut(s) 957
BtsIMutI CAGTG 2 cut(s) 880, 957
Cac8I GCNNGC 6 cut(s) 25, 642, 835, 910, 1316, 1384
CaiI CAGNNNCTG 1 cut(s) 1271
CfoI GCGC 5 cut(s) 412, 428, 991, 1031, 1074
Cfr10I RCCGGY 5 cut(s) 180, 1289, 1314, 1538, 1577
Cfr13I GGNCC 6 cut(s) 315, 507, 965, 1180, 1312, 1454
CseI GACGC 1 cut(s) 1512
Csp6I GTAC 4 cut(s) 117, 1259, 1330, 1467
CspAI ACCGGT 2 cut(s) 180, 1577
CspCI CAANNNNNGTGG 2 cut(s) 648, 683
CviAII CATG 6 cut(s) 623, 820, 923, 1057, 1153, 1499
CviQI GTAC 4 cut(s) 117, 1259, 1330, 1467
DdeI CTNAG 6 cut(s) 376, 422, 715, 992, 1254, 1418
DpnI GATC 3 cut(s) 685, 997, 1244
DpnII GATC 3 cut(s) 683, 995, 1242
DrdI GACNNNNNNGTC 1 cut(s) 1013
DseDI GACNNNNNNGTC 1 cut(s) 1013
EaeI YGGCCR 3 cut(s) 277, 1287, 1316
EagI CGGCCG 1 cut(s) 1316
Eam1104I CTCTTC 1 cut(s) 730
EarI CTCTTC 1 cut(s) 730
Ecl136II GAGCTC 2 cut(s) 245, 529
EclXI CGGCCG 1 cut(s) 1316
Eco105I TACGTA 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 2 cut(s) 247, 531
Eco47I GGWCC 3 cut(s) 507, 965, 1454
Eco47III AGCGCT 1 cut(s) 427
Eco52I CGGCCG 1 cut(s) 1316
Eco53kI GAGCTC 2 cut(s) 245, 529
Eco72I CACGTG 1 cut(s) 450
EcoICRI GAGCTC 2 cut(s) 245, 529
EcoRII CCWGG 3 cut(s) 1035, 1456, 1489
EcoT14I CCWWGG 1 cut(s) 234
EcoT38I GRGCYC 2 cut(s) 247, 531
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 6 cut(s) 626, 823, 926, 1060, 1156, 1502
FatI CATG 6 cut(s) 622, 819, 922, 1056, 1152, 1498
FauI CCCGC 2 cut(s) 32, 1375
FblI GTMKAC 3 cut(s) 112, 777, 1062
Fnu4HI GCNGC 8 cut(s) 429, 711, 723, 726, 1131, 1134, 1266, 1269
FokI GGATG 3 cut(s) 161, 988, 1336
FriOI GRGCYC 2 cut(s) 247, 531
FseI GGCCGGCC 1 cut(s) 1318
Fsp4HI GCNGC 8 cut(s) 429, 711, 723, 726, 1131, 1134, 1266, 1269
FspBI CTAG 5 cut(s) 80, 105, 861, 1335, 1470
FspI TGCGCA 1 cut(s) 411
GlaI GCGC 5 cut(s) 411, 427, 990, 1030, 1073
GluI GCNGC 8 cut(s) 429, 711, 723, 726, 1131, 1134, 1266, 1269
GsaI CCCAGC 1 cut(s) 1072
HaeII RGCGCY 3 cut(s) 429, 992, 1075
HaeIII GGCC 9 cut(s) 233, 279, 317, 978, 1182, 1289, 1314, 1318, 1489
HapII CCGG 9 cut(s) 181, 628, 782, 963, 1290, 1315, 1379, 1539, 1578
HgaI GACGC 1 cut(s) 1512
HhaI GCGC 5 cut(s) 412, 428, 991, 1031, 1074
Hin1I GRCGYC 1 cut(s) 1523
Hin1II CATG 6 cut(s) 626, 823, 926, 1060, 1156, 1502
Hin6I GCGC 5 cut(s) 410, 426, 989, 1029, 1072
HinP1I GCGC 5 cut(s) 410, 426, 989, 1029, 1072
HincII GTYRAC 2 cut(s) 349, 1189
HindII GTYRAC 2 cut(s) 349, 1189
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 3 cut(s) 146, 738, 927
HpaII CCGG 9 cut(s) 181, 628, 782, 963, 1290, 1315, 1379, 1539, 1578
HphI GGTGA 5 cut(s) 83, 131, 427, 1498, 1525
Hpy166II GTNNAC 7 cut(s) 113, 349, 778, 1063, 1189, 1533, 1590
Hpy188I TCNGA 6 cut(s) 205, 212, 526, 995, 1006, 1446
Hpy188III TCNNGA 4 cut(s) 248, 337, 742, 1361
Hpy8I GTNNAC 7 cut(s) 113, 349, 778, 1063, 1189, 1533, 1590
HpyAV CCTTC 4 cut(s) 277, 365, 872, 1042
HpyCH4III ACNGT 5 cut(s) 346, 542, 703, 812, 920
HpyCH4IV ACGT 5 cut(s) 115, 119, 449, 477, 1191
HpyCH4V TGCA 8 cut(s) 126, 153, 640, 722, 902, 1136, 1152, 1327
HpyF10VI GCNNNNNNNGC 7 cut(s) 159, 251, 285, 387, 418, 719, 1324
HpyF3I CTNAG 6 cut(s) 376, 422, 715, 992, 1254, 1418
HpySE526I ACGT 5 cut(s) 115, 119, 449, 477, 1191
Hsp92I GRCGYC 1 cut(s) 1523
Hsp92II CATG 6 cut(s) 626, 823, 926, 1060, 1156, 1502
HspAI GCGC 5 cut(s) 410, 426, 989, 1029, 1072
KroI GCCGGC 1 cut(s) 1314
KroNI GCCGGC 1 cut(s) 1316
Kzo9I GATC 3 cut(s) 683, 995, 1242
LmnI GCTCC 1 cut(s) 526
Lsp1109I GCAGC 5 cut(s) 415, 734, 1120, 1142, 1277
LweI GCATC 8 cut(s) 183, 241, 397, 709, 951, 1010, 1016, 1314
MaeI CTAG 5 cut(s) 80, 105, 861, 1335, 1470
MaeII ACGT 5 cut(s) 115, 119, 449, 477, 1191
MaeIII GTNAC 7 cut(s) 89, 361, 478, 512, 581, 881, 1124
MalI GATC 3 cut(s) 685, 997, 1244
MboI GATC 3 cut(s) 683, 995, 1242
MboII GAAGA 5 cut(s) 58, 587, 747, 778, 1009
MhlI GDGCHC 2 cut(s) 247, 531
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 9 cut(s) 17, 400, 607, 895, 1147, 1236, 1249, 1366, 1408
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 2 cut(s) 155, 747
MmeI TCCRAC 2 cut(s) 504, 1029
MnlI CCTC 7 cut(s) 147, 211, 443, 624, 723, 1019, 1064
Mox20I TGGCCA 1 cut(s) 279
MroNI GCCGGC 1 cut(s) 1314
MroXI GAANNNNTTC 2 cut(s) 332, 600
MscI TGGCCA 1 cut(s) 279
MslI CAYNNNNRTG 2 cut(s) 1055, 1580
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 1133, 1271
MspI CCGG 9 cut(s) 181, 628, 782, 963, 1290, 1315, 1379, 1539, 1578
MspR9I CCNGG 4 cut(s) 782, 1037, 1458, 1491
Mva1269I GAATGC 1 cut(s) 1094
MvaI CCWGG 3 cut(s) 1037, 1458, 1491
MvnI CGCG 2 cut(s) 311, 728
MwoI GCNNNNNNNGC 7 cut(s) 159, 251, 285, 387, 418, 719, 1324
NaeI GCCGGC 1 cut(s) 1316
NciI CCSGG 1 cut(s) 782
NdeII GATC 3 cut(s) 683, 995, 1242
NgoMIV GCCGGC 1 cut(s) 1314
NlaIII CATG 6 cut(s) 626, 823, 926, 1060, 1156, 1502
NlaIV GGNNCC 3 cut(s) 185, 1105, 1181
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 1 cut(s) 89
NsbI TGCGCA 1 cut(s) 411
NspV TTCGAA 1 cut(s) 617
OliI CACNNNNGTG 1 cut(s) 1580
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PctI GAATGC 1 cut(s) 1094
PdiI GCCGGC 1 cut(s) 1316
PdmI GAANNNNTTC 2 cut(s) 332, 600
PfeI GAWTC 1 cut(s) 927
Pfl23II CGTACG 1 cut(s) 116
PflMI CCANNNNNTGG 1 cut(s) 985
PinAI ACCGGT 2 cut(s) 180, 1577
PkrI GCNGC 8 cut(s) 430, 712, 724, 727, 1132, 1135, 1267, 1270
PleI GAGTC 2 cut(s) 154, 746
PmaCI CACGTG 1 cut(s) 450
PmlI CACGTG 1 cut(s) 450
PpsI GAGTC 2 cut(s) 154, 746
Ppu21I YACGTR 3 cut(s) 116, 120, 450
PsiI TTATAA 1 cut(s) 755
Psp124BI GAGCTC 2 cut(s) 247, 531
Psp1406I AACGTT 1 cut(s) 477
Psp6I CCWGG 3 cut(s) 1035, 1456, 1489
PspCI CACGTG 1 cut(s) 450
PspFI CCCAGC 1 cut(s) 1068
PspGI CCWGG 3 cut(s) 1035, 1456, 1489
PspLI CGTACG 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 185, 1105, 1181
PspPI GGNCC 6 cut(s) 315, 507, 965, 1180, 1312, 1454
PstI CTGCAG 1 cut(s) 1138
PstNI CAGNNNCTG 1 cut(s) 1271
PvuII CAGCTG 1 cut(s) 1133
RigI GGCCGGCC 1 cut(s) 1318
RsaI GTAC 4 cut(s) 118, 1260, 1331, 1468
RsaNI GTAC 4 cut(s) 117, 1259, 1330, 1467
RseI CAYNNNNRTG 2 cut(s) 1055, 1580
SacI GAGCTC 2 cut(s) 247, 531
SatI GCNGC 8 cut(s) 429, 711, 723, 726, 1131, 1134, 1266, 1269
Sau3AI GATC 3 cut(s) 683, 995, 1242
Sau96I GGNCC 6 cut(s) 315, 507, 965, 1180, 1312, 1454
ScaI AGTACT 1 cut(s) 1331
SchI GAGTC 2 cut(s) 155, 747
ScrFI CCNGG 4 cut(s) 782, 1037, 1458, 1491
SduI GDGCHC 2 cut(s) 247, 531
SfaNI GCATC 8 cut(s) 183, 241, 397, 709, 951, 1010, 1016, 1314
SfcI CTRYAG 2 cut(s) 1081, 1134
SfuI TTCGAA 1 cut(s) 617
SgrAI CRCCGGYG 1 cut(s) 1577
SinI GGWCC 3 cut(s) 507, 965, 1454
SmiMI CAYNNNNRTG 2 cut(s) 1055, 1580
SnaBI TACGTA 1 cut(s) 116
Sse9I AATT 9 cut(s) 17, 400, 607, 895, 1147, 1236, 1249, 1366, 1408
SsiI CCGC 9 cut(s) 25, 97, 309, 711, 726, 1269, 1382, 1393, 1536
SspMI CTAG 5 cut(s) 80, 105, 861, 1335, 1470
SstI GAGCTC 2 cut(s) 247, 531
StyD4I CCNGG 4 cut(s) 780, 1035, 1456, 1489
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 5 cut(s) 346, 542, 703, 812, 920
TaiI ACGT 5 cut(s) 118, 122, 452, 480, 1194
TaqI TCGA 7 cut(s) 167, 249, 336, 617, 698, 1210, 1241
TaqII GACCGA 1 cut(s) 1003
TasI AATT 9 cut(s) 17, 400, 607, 895, 1147, 1236, 1249, 1366, 1408
TatI WGTACW 2 cut(s) 1329, 1466
TauI GCSGC 3 cut(s) 713, 728, 1271
TfiI GAWTC 1 cut(s) 927
TscAI CASTG 2 cut(s) 880, 964
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 5 cut(s) 428, 722, 1130, 1133, 1265
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 6 cut(s) 43, 478, 919, 1105, 1364, 1487
TspGWI ACGGA 2 cut(s) 497, 587
TspRI CASTG 2 cut(s) 880, 964
Van91I CCANNNNNTGG 1 cut(s) 985
VpaK11BI GGWCC 3 cut(s) 507, 965, 1454
XapI RAATTY 1 cut(s) 17
XcmI CCANNNNNNNNNTGG 1 cut(s) 1516
XmiI GTMKAC 3 cut(s) 112, 777, 1062
XmnI GAANNNNTTC 2 cut(s) 332, 600
XspI CTAG 5 cut(s) 80, 105, 861, 1335, 1470
ZrmI AGTACT 1 cut(s) 1331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.