AT5G51490

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016037
Physical Location & Seq
Reverse (-)
20913510 .. 20915879
2370 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G51490.1

Sequence Viewer

Length: 1611 bp
ATGAATATGATGATGCAAAAACTCTCTATTCTATTTCTACATTTAATATTACTAGTACTACTCTGCGTCCATCCTCTCACCACCGTAGCCGACCGCAATTCCACCGATTGGTGCGACAAAACTCCATACCCTGATCCATGCAAATGCTACTTCAAGAACCACAACGGATTTCAACAGCCGACACAATTATCCGAGTTCCGAGTAATGCTGGTGGAAGCAGCCATGGATCGGGCCATATCCGCCCGTGCCGAGTTGACTAATTCCGGCAAGAACTGCACGGATAGCAAGAAACAAGCAGTTTTGGCGGATTGCATTGACCTCTATGGAGACACGATCATGCAGTTAAACCGGACATTGCATGGCGTGTCTCCTAAAGCCGGTGCCGCGAAAAGTTGCACTGACTTCGACGCTCAAACGTGGCTGAGCACCGCGCTTACAAACACCGAGACCTGCCGACGTGGCTCATCCGATTTAAACGTCACAGACTTCATCACACCCATCGTTTCAAACACCAAAATCTCCCACCTCATTAGCAACTGCTTAGCCGTCAACGGAGCCCTCTTGACCGCTGGAAACAAAGGCAACACCACTGCTAATCAAAAGGGCTTTCCGACGTGGCTTTCCCGTAAAGATAAGAGACTCCTGCGTGCCGTTCGAGCCAACCTCGTGGTGGCCAAGGACGGATCAGGACATTTCAACACGGTACAAGCGGCTATTGACGTGGCGGGACGGAGAAAGGTGACGTCAGGGAGGTTCGTTATATACGTAAAAAGAGGTATATATCAAGAAAACATTAACGTACGTCTAAATAATGATGACATAATGTTGGTCGGAGATGGAATGAGATCCACCATTATCACCGGCGGTCGAAGTGTCCAAGGAGGTTACACCACGTACAATTCCGCTACTGCCGGTATCGAGGGACTTCACTTCATTGCCAAAGGCATAACGTTTAGGAACACAGCTGGTCCAGCCAAGGGCCAGGCCGTGGCACTTCGTTCATCGTCTGACCTCTCAATCTTCTACAAATGCTCAATCGAAGGATACCAAGATACACTGATGGTCCATTCTCAACGCCAGTTTTACCGTGAGTGTTACATCTATGGAACCGTCGATTTCATATTCGGTAACGCGGCTGCTGTTTTCCAAAACTGTCTCATCCTCCCTCGCCGGCCACTCAAAGGTCAAGCCAATGTAATTACCGCACAAGGTCGTGCTGATCCGTTTCAGAACACAGGGATCTCTATCCATAACTCAAGAATCCTACCGGCTCCTGATCTAAAACCAGTGGTCGGTACCGTTAAGACGTACATGGGCCGGCCTTGGATGAAGTTCTCACGAACCGTGGTTCTTCAGACGTATTTGGATAATGTTGTGAGTCCAGTCGGGTGGTCTCCGTGGATTGAAGGTTCGGTGTTTGGTCTGGACACTCTCTTCTATGCAGAATATAAGAATACTGGACCAGCTTCATCCACGAGGTGGCGTGTCAGTTGGAAAGGGTTTCATGTGTTGGGTAGAGCTTCCGATGCATCTGCTTTCACTGTTGGAAAGTTTATCGCTGGTACAGCATGGCTCCCACGCACCGGCATACCCTTCACTTCTGGACTCTAA

Protein Analysis

536

Amino Acids

58.87

Weight (kDa)

9.56

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 36 - 183 5e-29 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 221 - 521 2.1e-129 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47400 AT5G51490 AT5G51500
fragaria_vesca FvH4_7g27770
malus_domestica MD11G1195100.v1.1 MD11G1195400.v1.1
prunus_persica Prupe.2G278300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0374561
rosa_laevigata RLG00000026728
rosa_roxburghii Rroxscaffold_4G00283400
rosa_samantha Rh1AG394900 Rh1BG359600 Rh1CG372700 Rh1DG390200
rosa_wichuraiana Rw1G035020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 746
Acc36I ACCTGC 1 cut(s) 458
Acc65I GGTACC 1 cut(s) 1295
AccB1I GGYRCC 2 cut(s) 380, 1295
AccB7I CCANNNNNTGG 3 cut(s) 108, 988, 1479
AccII CGCG 3 cut(s) 386, 431, 1133
AclI AACGTT 1 cut(s) 950
AclWI GGATC 6 cut(s) 128, 234, 691, 840, 1214, 1247
AcoI YGGCCR 2 cut(s) 672, 1172
AcuI CTGAAG 1 cut(s) 1337
AcyI GRCGYC 1 cut(s) 743
AdeI CACNNNGTG 1 cut(s) 1479
AfaI GTAC 7 cut(s) 57, 705, 801, 896, 1297, 1310, 1564
AgsI TTSAA 5 cut(s) 154, 173, 507, 697, 1406
AhlI ACTAGT 1 cut(s) 52
AjiI CACGTC 3 cut(s) 458, 615, 721
AjnI CCWGG 1 cut(s) 981
AloI GAACNNNNNNTCC 2 cut(s) 1393, 1425
AluBI AGCT 3 cut(s) 965, 1466, 1520
AluI AGCT 3 cut(s) 965, 1466, 1520
Alw21I GWGCWC 1 cut(s) 428
Alw26I GTCTC 6 cut(s) 321, 372, 440, 631, 1160, 1398
AlwI GGATC 6 cut(s) 128, 234, 691, 840, 1214, 1247
AoxI GGCC 7 cut(s) 231, 672, 979, 984, 1172, 1315, 1319
ApeKI GCWGC 2 cut(s) 218, 1136
Asp718I GGTACC 1 cut(s) 1295
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 6 cut(s) 231, 968, 979, 1063, 1315, 1460
AsuHPI GGTGA 3 cut(s) 70, 751, 850
AvaII GGWCC 3 cut(s) 968, 1063, 1460
BalI TGGCCA 1 cut(s) 674
BanI GGYRCC 2 cut(s) 380, 1295
BanII GRGCYC 1 cut(s) 559
BauI CACGAG 2 cut(s) 665, 1474
Bbv12I GWGCWC 1 cut(s) 428
BbvI GCAGC 2 cut(s) 230, 1123
BccI CCATC 4 cut(s) 78, 506, 830, 1054
BceAI ACGGC 3 cut(s) 530, 635, 971
BcgI CGANNNNNNTGC 4 cut(s) 385, 419, 1514, 1548
BciT130I CCWGG 1 cut(s) 983
BciVI GTATCC 1 cut(s) 1037
BcoDI GTCTC 6 cut(s) 321, 372, 440, 631, 1160, 1398
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 458
BfuI GTATCC 1 cut(s) 1037
BglI GCCNNNNNGGC 1 cut(s) 459
BisI GCNGC 5 cut(s) 219, 384, 711, 1134, 1137
BlpI GCTNAGC 2 cut(s) 422, 541
BlsI GCNGC 5 cut(s) 220, 385, 712, 1135, 1138
BmcAI AGTACT 1 cut(s) 57
Bme1390I CCNGG 1 cut(s) 983
Bme18I GGWCC 3 cut(s) 968, 1063, 1460
BmgBI CACGTC 3 cut(s) 458, 615, 721
BmgT120I GGNCC 6 cut(s) 231, 968, 979, 1063, 1315, 1460
BmiI GGNNCC 6 cut(s) 382, 556, 1108, 1272, 1297, 1574
BmrFI CCNGG 1 cut(s) 983
BmsI GCATC 3 cut(s) 3, 1516, 1538
BplI GAGNNNNNCTC 2 cut(s) 648, 680
Bpu1102I GCTNAGC 2 cut(s) 422, 541
BpuEI CTTGAG 1 cut(s) 1240
BsaAI YACGTR 2 cut(s) 766, 894
BsaBI GATNNNNATC 1 cut(s) 1244
BsaHI GRCGYC 1 cut(s) 743
BsaI GGTCTC 2 cut(s) 440, 1398
BsaJI CCNNGG 8 cut(s) 222, 675, 877, 975, 987, 1322, 1344, 1397
BsaWI WCCGGW 1 cut(s) 348
Bse118I RCCGGY 7 cut(s) 377, 860, 911, 1170, 1267, 1317, 1583
Bse1I ACTGG 4 cut(s) 1078, 1286, 1382, 1462
Bse3DI GCAATG 2 cut(s) 353, 933
Bse8I GATNNNNATC 1 cut(s) 1244
BseBI CCWGG 1 cut(s) 983
BseDI CCNNGG 8 cut(s) 222, 675, 877, 975, 987, 1322, 1344, 1397
BseGI GGATG 5 cut(s) 70, 464, 1158, 1332, 1469
BseJI GATNNNNATC 1 cut(s) 1244
BseMI GCAATG 2 cut(s) 353, 933
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 4 cut(s) 1078, 1286, 1382, 1462
BseXI GCAGC 2 cut(s) 230, 1123
BsgI GTGCAG 1 cut(s) 259
Bsh1236I CGCG 3 cut(s) 386, 431, 1133
Bsh1285I CGRYCG 2 cut(s) 94, 868
BshFI GGCC 7 cut(s) 233, 674, 981, 986, 1174, 1317, 1321
BshNI GGYRCC 2 cut(s) 380, 1295
BsiEI CGRYCG 2 cut(s) 94, 868
BsiHKAI GWGCWC 1 cut(s) 428
BsiSI CCGG 9 cut(s) 264, 349, 378, 861, 912, 1171, 1268, 1318, 1584
BsiWI CGTACG 1 cut(s) 799
BslFI GGGAC 2 cut(s) 741, 936
BsmAI GTCTC 6 cut(s) 321, 372, 440, 631, 1160, 1398
BsmFI GGGAC 2 cut(s) 741, 936
BsnI GGCC 7 cut(s) 233, 674, 981, 986, 1174, 1317, 1321
Bso31I GGTCTC 2 cut(s) 440, 1398
Bsp1286I GDGCHC 2 cut(s) 428, 559
Bsp143I GATC 8 cut(s) 133, 226, 333, 683, 845, 1219, 1239, 1276
Bsp1720I GCTNAGC 2 cut(s) 422, 541
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 7 cut(s) 233, 674, 981, 986, 1174, 1317, 1321
BspCNI CTCAG 1 cut(s) 414
BspFNI CGCG 3 cut(s) 386, 431, 1133
BspLI GGNNCC 6 cut(s) 382, 556, 1108, 1272, 1297, 1574
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 6 cut(s) 128, 234, 691, 840, 1214, 1247
BspT107I GGYRCC 2 cut(s) 380, 1295
BspTNI GGTCTC 2 cut(s) 440, 1398
BsrDI GCAATG 2 cut(s) 353, 933
BsrFI RCCGGY 7 cut(s) 377, 860, 911, 1170, 1267, 1317, 1583
BsrI ACTGG 4 cut(s) 1078, 1286, 1382, 1462
BssAI RCCGGY 7 cut(s) 377, 860, 911, 1170, 1267, 1317, 1583
BssECI CCNNGG 8 cut(s) 222, 675, 877, 975, 987, 1322, 1344, 1397
BssMI GATC 8 cut(s) 133, 226, 333, 683, 845, 1219, 1239, 1276
BssNI GRCGYC 1 cut(s) 743
BssSI CACGAG 2 cut(s) 665, 1474
BssT1I CCWWGG 5 cut(s) 222, 675, 877, 975, 1322
Bst2BI CACGAG 2 cut(s) 665, 1474
Bst2UI CCWGG 1 cut(s) 983
Bst4CI ACNGT 8 cut(s) 85, 703, 1088, 1111, 1154, 1300, 1345, 1543
Bst6I CTCTTC 1 cut(s) 1439
BstACI GRCGYC 1 cut(s) 743
BstAPI GCANNNNNTGC 1 cut(s) 273
BstBAI YACGTR 2 cut(s) 766, 894
BstC8I GCNNGC 3 cut(s) 648, 1172, 1319
BstDEI CTNAG 2 cut(s) 422, 541
BstDSI CCRYGG 4 cut(s) 222, 987, 1344, 1397
BstF5I GGATG 5 cut(s) 70, 464, 1158, 1332, 1469
BstFNI CGCG 3 cut(s) 386, 431, 1133
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 8 cut(s) 136, 229, 336, 686, 848, 1222, 1242, 1279
BstMAI GTCTC 6 cut(s) 321, 372, 440, 631, 1160, 1398
BstMBI GATC 8 cut(s) 133, 226, 333, 683, 845, 1219, 1239, 1276
BstMCI CGRYCG 2 cut(s) 94, 868
BstNI CCWGG 1 cut(s) 983
BstSCI CCNGG 1 cut(s) 981
BstSNI TACGTA 1 cut(s) 766
BstUI CGCG 3 cut(s) 386, 431, 1133
BstV1I GCAGC 2 cut(s) 230, 1123
BstX2I RGATCY 2 cut(s) 845, 1239
BstXI CCANNNNNNTGG 2 cut(s) 667, 1389
BstYI RGATCY 2 cut(s) 845, 1239
BsuI GTATCC 1 cut(s) 1037
BsuRI GGCC 7 cut(s) 233, 674, 981, 986, 1174, 1317, 1321
BtgI CCRYGG 4 cut(s) 222, 987, 1344, 1397
BtrI CACGTC 3 cut(s) 458, 615, 721
BtsCI GGATG 5 cut(s) 70, 464, 1158, 1332, 1469
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 5 cut(s) 396, 588, 1055, 1293, 1539
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 3 cut(s) 648, 1172, 1319
CfoI GCGC 1 cut(s) 433
Cfr10I RCCGGY 7 cut(s) 377, 860, 911, 1170, 1267, 1317, 1583
Cfr13I GGNCC 6 cut(s) 231, 968, 979, 1063, 1315, 1460
CseI GACGC 2 cut(s) 55, 416
Csp6I GTAC 7 cut(s) 56, 704, 800, 895, 1296, 1309, 1563
CviAII CATG 7 cut(s) 138, 223, 337, 359, 1312, 1505, 1569
CviQI GTAC 7 cut(s) 56, 704, 800, 895, 1296, 1309, 1563
DdeI CTNAG 2 cut(s) 422, 541
DpnI GATC 8 cut(s) 135, 228, 335, 685, 847, 1221, 1241, 1278
DpnII GATC 8 cut(s) 133, 226, 333, 683, 845, 1219, 1239, 1276
DraI TTTAAA 1 cut(s) 474
DraIII CACNNNGTG 1 cut(s) 1479
EaeI YGGCCR 2 cut(s) 672, 1172
Eam1104I CTCTTC 1 cut(s) 1439
EarI CTCTTC 1 cut(s) 1439
EciI GGCGGA 2 cut(s) 229, 320
Eco105I TACGTA 1 cut(s) 766
Eco130I CCWWGG 5 cut(s) 222, 675, 877, 975, 1322
Eco24I GRGCYC 1 cut(s) 559
Eco31I GGTCTC 2 cut(s) 440, 1398
Eco47I GGWCC 3 cut(s) 968, 1063, 1460
Eco57I CTGAAG 1 cut(s) 1337
EcoRII CCWGG 1 cut(s) 981
EcoT14I CCWWGG 5 cut(s) 222, 675, 877, 975, 1322
EcoT22I ATGCAT 1 cut(s) 1531
EcoT38I GRGCYC 1 cut(s) 559
ErhI CCWWGG 5 cut(s) 222, 675, 877, 975, 1322
FaeI CATG 7 cut(s) 141, 226, 340, 362, 1315, 1508, 1572
FaqI GGGAC 2 cut(s) 741, 936
FatI CATG 7 cut(s) 137, 222, 336, 358, 1311, 1504, 1568
FauI CCCGC 1 cut(s) 718
Fnu4HI GCNGC 5 cut(s) 219, 384, 711, 1134, 1137
FokI GGATG 5 cut(s) 57, 451, 1145, 1339, 1456
FriOI GRGCYC 1 cut(s) 559
FseI GGCCGGCC 1 cut(s) 1321
Fsp4HI GCNGC 5 cut(s) 219, 384, 711, 1134, 1137
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 432
GluI GCNGC 5 cut(s) 219, 384, 711, 1134, 1137
HaeIII GGCC 7 cut(s) 233, 674, 981, 986, 1174, 1317, 1321
HapII CCGG 9 cut(s) 264, 349, 378, 861, 912, 1171, 1268, 1318, 1584
HgaI GACGC 2 cut(s) 55, 416
HhaI GCGC 1 cut(s) 433
Hin1I GRCGYC 1 cut(s) 743
Hin1II CATG 7 cut(s) 141, 226, 340, 362, 1315, 1508, 1572
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HincII GTYRAC 2 cut(s) 255, 550
HindII GTYRAC 2 cut(s) 255, 550
HinfI GANTC 4 cut(s) 639, 1260, 1378, 1605
HpaII CCGG 9 cut(s) 264, 349, 378, 861, 912, 1171, 1268, 1318, 1584
HphI GGTGA 3 cut(s) 70, 751, 850
Hpy166II GTNNAC 2 cut(s) 255, 550
Hpy188I TCNGA 9 cut(s) 193, 200, 469, 612, 833, 1009, 1230, 1356, 1525
Hpy188III TCNNGA 9 cut(s) 154, 562, 687, 785, 1257, 1274, 1338, 1424, 1602
Hpy8I GTNNAC 2 cut(s) 255, 550
Hpy99I CGWCG 4 cut(s) 410, 459, 616, 1115
HpyAV CCTTC 3 cut(s) 1034, 1400, 1603
HpyCH4III ACNGT 8 cut(s) 85, 703, 1088, 1111, 1154, 1300, 1345, 1543
HpyCH4V TGCA 9 cut(s) 16, 141, 276, 312, 340, 358, 396, 1442, 1529
HpyF3I CTNAG 2 cut(s) 422, 541
Hsp92I GRCGYC 1 cut(s) 743
Hsp92II CATG 7 cut(s) 141, 226, 340, 362, 1315, 1508, 1572
HspAI GCGC 1 cut(s) 431
KpnI GGTACC 1 cut(s) 1299
KroI GCCGGC 2 cut(s) 1170, 1317
KroNI GCCGGC 2 cut(s) 1172, 1319
Kzo9I GATC 8 cut(s) 133, 226, 333, 683, 845, 1219, 1239, 1276
LmnI GCTCC 3 cut(s) 554, 1276, 1578
Lsp1109I GCAGC 2 cut(s) 230, 1123
LweI GCATC 3 cut(s) 3, 1516, 1538
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 5 cut(s) 478, 739, 884, 1094, 1127
MalI GATC 8 cut(s) 135, 228, 335, 685, 847, 1221, 1241, 1278
MboI GATC 8 cut(s) 133, 226, 333, 683, 845, 1219, 1239, 1276
MboII GAAGA 3 cut(s) 1012, 1343, 1426
MflI RGATCY 2 cut(s) 845, 1239
MhlI GDGCHC 2 cut(s) 428, 559
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 5 cut(s) 97, 185, 259, 898, 1197
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 3 cut(s) 633, 1387, 1599
MmeI TCCRAC 4 cut(s) 635, 811, 1472, 1525
Mox20I TGGCCA 1 cut(s) 674
Mph1103I ATGCAT 1 cut(s) 1531
MroNI GCCGGC 2 cut(s) 1170, 1317
MscI TGGCCA 1 cut(s) 674
MseI TTAA 5 cut(s) 44, 344, 473, 795, 1302
MslI CAYNNNNRTG 2 cut(s) 142, 335
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 2 cut(s) 569, 965
MspI CCGG 9 cut(s) 264, 349, 378, 861, 912, 1171, 1268, 1318, 1584
MspR9I CCNGG 1 cut(s) 983
MvaI CCWGG 1 cut(s) 983
MvnI CGCG 3 cut(s) 386, 431, 1133
NaeI GCCGGC 2 cut(s) 1172, 1319
NcoI CCATGG 1 cut(s) 222
NdeII GATC 8 cut(s) 133, 226, 333, 683, 845, 1219, 1239, 1276
NgoMIV GCCGGC 2 cut(s) 1170, 1317
NlaIII CATG 7 cut(s) 141, 226, 340, 362, 1315, 1508, 1572
NlaIV GGNNCC 6 cut(s) 382, 556, 1108, 1272, 1297, 1574
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 2 cut(s) 478, 739
NsiI ATGCAT 1 cut(s) 1531
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PdiI GCCGGC 2 cut(s) 1172, 1319
PfeI GAWTC 1 cut(s) 1260
Pfl23II CGTACG 1 cut(s) 799
PflMI CCANNNNNTGG 3 cut(s) 108, 988, 1479
PkrI GCNGC 5 cut(s) 220, 385, 712, 1135, 1138
PleI GAGTC 3 cut(s) 633, 1386, 1599
PpsI GAGTC 3 cut(s) 633, 1386, 1599
Ppu21I YACGTR 2 cut(s) 766, 894
Psp1406I AACGTT 1 cut(s) 950
Psp6I CCWGG 1 cut(s) 981
PspGI CCWGG 1 cut(s) 981
PspLI CGTACG 1 cut(s) 799
PspN4I GGNNCC 6 cut(s) 382, 556, 1108, 1272, 1297, 1574
PspPI GGNCC 6 cut(s) 231, 968, 979, 1063, 1315, 1460
PsuI RGATCY 2 cut(s) 845, 1239
PvuII CAGCTG 1 cut(s) 965
RigI GGCCGGCC 1 cut(s) 1321
RsaI GTAC 7 cut(s) 57, 705, 801, 896, 1297, 1310, 1564
RsaNI GTAC 7 cut(s) 56, 704, 800, 895, 1296, 1309, 1563
RseI CAYNNNNRTG 2 cut(s) 142, 335
SaqAI TTAA 5 cut(s) 44, 344, 473, 795, 1302
SatI GCNGC 5 cut(s) 219, 384, 711, 1134, 1137
Sau3AI GATC 8 cut(s) 133, 226, 333, 683, 845, 1219, 1239, 1276
Sau96I GGNCC 6 cut(s) 231, 968, 979, 1063, 1315, 1460
ScaI AGTACT 1 cut(s) 57
SchI GAGTC 3 cut(s) 633, 1387, 1599
ScrFI CCNGG 1 cut(s) 983
SduI GDGCHC 2 cut(s) 428, 559
SfaNI GCATC 3 cut(s) 3, 1516, 1538
SgrAI CRCCGGYG 1 cut(s) 860
SinI GGWCC 3 cut(s) 968, 1063, 1460
SmiMI CAYNNNNRTG 2 cut(s) 142, 335
SmlI CTYRAG 1 cut(s) 1255
SmoI CTYRAG 1 cut(s) 1255
SnaBI TACGTA 1 cut(s) 766
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 5 cut(s) 97, 185, 259, 898, 1197
SspI AATATT 1 cut(s) 48
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 981
StyI CCWWGG 5 cut(s) 222, 675, 877, 975, 1322
TaaI ACNGT 8 cut(s) 85, 703, 1088, 1111, 1154, 1300, 1345, 1543
TaqI TCGA 6 cut(s) 405, 655, 868, 918, 1038, 1113
TasI AATT 5 cut(s) 97, 185, 259, 898, 1197
TatI WGTACW 1 cut(s) 55
TauI GCSGC 3 cut(s) 386, 713, 1136
TfiI GAWTC 1 cut(s) 1260
Tru1I TTAA 5 cut(s) 44, 344, 473, 795, 1302
Tru9I TTAA 5 cut(s) 44, 344, 473, 795, 1302
TscAI CASTG 5 cut(s) 403, 595, 1062, 1293, 1546
TseFI GTSAC 2 cut(s) 478, 739
TseI GCWGC 2 cut(s) 218, 1136
Tsp45I GTSAC 2 cut(s) 478, 739
TspDTI ATGAA 8 cut(s) 17, 478, 922, 990, 1108, 1343, 1458, 1493
TspGWI ACGGA 7 cut(s) 180, 293, 567, 696, 745, 1212, 1386
TspRI CASTG 5 cut(s) 403, 595, 1062, 1293, 1546
Van91I CCANNNNNTGG 3 cut(s) 108, 988, 1479
VpaK11BI GGWCC 3 cut(s) 968, 1063, 1460
XcmI CCANNNNNNNNNTGG 1 cut(s) 667
XspI CTAG 1 cut(s) 53
ZraI GACGTC 1 cut(s) 744
ZrmI AGTACT 1 cut(s) 57
Zsp2I ATGCAT 1 cut(s) 1531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.