Rw1G035020

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016037
Physical Location & Seq
Reverse (-)
62886863 .. 62888975
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035020.1

Sequence Viewer

Length: 1608 bp
ATGGCTATGAAACTAACTTTCCTACACTCTTTACTCATTTTCTCATCCATGCTCTTTCAGACAGCCCTATCAAGAAGCCACCACCATCCCTCCAATCGCATTCGAACATGGTGCAACAAAACCCCTCACCCGGAGCCATGCAAATACTGGATGTCCCATTACCCCCACCGTCTCACCCCGAAGCACTCCACCGATTTCCGAAAGCTGATGGTCCAGGTTGCCCTCGAGAGAGCCCTCAGTGCTAAATCATACGCCTCCCAATTCAAGCAAAACTGCGAAAGCGATCCCCAAAAGGCTGCGTGGACCGATTGCTTGAAGCTCTTCGACAACACGGTCCACCAGCTCAACCGCACCCTGCAAGGCCTAGACACCAAGCATAAAGAGACCTGCACCAATTTTGATGCACAGACTTGGCTCAGCGCCGCCCTCACCTACATTCACACTTGCCGAGACGGATCCAAGGAACTGAACGTGTCGAACTTCATAACCCCTATTTATACGTCCAACAACAATGTGTCCGAGCTTATTAGCAACAGTTTGGCAATTGGTTCACAACTTCTGGAGAAAGAAAATCAAACAACCACAGACGAAGGGTATGAGTCTTTTCCCAGTTGGCTTTCAAACCACGACAGGAGGCTATTGGCAGCTTCTTCGATCAAGGCTAACCTCGTGGTGGCGAAAGACGGATCGGGCCACTTTCGGACCATCCAGGCGGCATTAAATTTGGCGGCAAAGCGAAAAAGAATGACCAGATTTGTTATCCATGTCAAAAAAGGTGTTTATAGGGAGAACATTGAAGTTGTCAACACCAACACTAACATCATGCTGGTTGGTGATAGCTTGAGGTATACGATAATCACAGGTGGCAGAAGTGTCAAAGGAGGTTACACTACTTTTAACTCTGCAACTGCAGGCATTGACGGGCCTGGCTTCATTGCCCGCGGCATCACATTCAGCAACACTGCCGGAGCACTAAAGGGACAAGCGGTGGCCCTTCGATCCAGCTCCGACCTTTCAGTATTCTACCGTTGCGCCTTCCAAGGCTACCAAGACACTCTCATGGTCCACTCTCAAAGACAATTCTACAGAGAATGTTACATCTATGGCACCATAGACTTTATATTTGGTAATGCTGCTGTTGTTTTCCAGAGTTGCATTATTTACGTAAGAAAACCCTTATGGGGCCAAGTCAATGTCATCACAGCCCAAGCCCGAAACGACCCGTTTCAAAGCACGGGTATTTCAATCCATAACTCTCAAGTTCGGGCTGCCCCAGACCTTAAGCCGGTGATTCGCTACTATAAAACATACTTGGGCCGGCCATGGATGCAATATTCTCGGGTTGTCTTCATGAAATGTTACCTTGATAGCTTGGTCGACCCGGCAGGTTGGCTGGAATGGCAAAGAACTAATTTTGCTCTAAGCACTTTGTATTATGGGGAGTACAAGAATTTTGGTCCTGCCTCGTCCACCAGATATAGGGTCAAGTGGCCTGGTTTTCATGTCATTACTAGTGCAAATGTGGCAACATCGTTCACCGTTGGAAGCCTTATTGCCGGACGATCGTGGCTGCCCACCACTGGTGTTCCGTTCACGTCAGGCCTTTGA

Protein Analysis

535

Amino Acids

60.33

Weight (kDa)

9.65

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 182 8e-37 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 222 - 520 1.9e-129 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47400 AT5G51490 AT5G51500
fragaria_vesca FvH4_7g27770
malus_domestica MD11G1195100.v1.1 MD11G1195400.v1.1
prunus_persica Prupe.2G278300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0374561
rosa_laevigata RLG00000026728
rosa_roxburghii Rroxscaffold_4G00283400
rosa_samantha Rh1AG394900 Rh1BG359600 Rh1CG372700 Rh1DG390200
rosa_wichuraiana Rw1G035020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
Acc36I ACCTGC 2 cut(s) 395, 1376
AccB1I GGYRCC 1 cut(s) 1106
AccI GTMKAC 2 cut(s) 848, 1377
AccII CGCG 1 cut(s) 942
AciI CCGC 7 cut(s) 349, 423, 713, 728, 940, 942, 986
AclWI GGATC 5 cut(s) 278, 450, 463, 694, 993
AcoI YGGCCR 1 cut(s) 1319
AcsI RAATTY 2 cut(s) 721, 1450
AfaI GTAC 1 cut(s) 1445
AfiI CCNNNNNNNGG 6 cut(s) 130, 673, 1181, 1285, 1479, 1580
AflII CTTAAG 1 cut(s) 1280
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 6 cut(s) 265, 316, 621, 797, 1229, 1245
AhlI ACTAGT 1 cut(s) 1511
AjiI CACGTC 1 cut(s) 1596
AjnI CCWGG 4 cut(s) 213, 708, 925, 1492
AluBI AGCT 8 cut(s) 205, 319, 343, 523, 647, 840, 1005, 1371
AluI AGCT 8 cut(s) 205, 319, 343, 523, 647, 840, 1005, 1371
Alw21I GWGCWC 1 cut(s) 973
Alw26I GTCTC 3 cut(s) 176, 377, 444
AlwI GGATC 5 cut(s) 278, 450, 463, 694, 993
Ama87I CYCGRG 2 cut(s) 224, 1338
AoxI GGCC 9 cut(s) 361, 691, 923, 990, 1183, 1315, 1319, 1490, 1600
ApeKI GCWGC 5 cut(s) 296, 644, 1133, 1268, 1570
ApoI RAATTY 2 cut(s) 721, 1450
ArsI GACNNNNNNTTYG 2 cut(s) 1106, 1138
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 2 cut(s) 422, 1034
AsuC2I CCSGG 2 cut(s) 131, 1382
AsuHPI GGTGA 6 cut(s) 119, 166, 421, 845, 1300, 1528
AsuII TTCGAA 1 cut(s) 103
AvaI CYCGRG 2 cut(s) 224, 1338
AvaII GGWCC 6 cut(s) 211, 303, 334, 702, 1063, 1457
BamHI GGATCC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 1106
BanII GRGCYC 1 cut(s) 235
BauI CACGAG 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 1339
Bbv12I GWGCWC 1 cut(s) 973
BbvI GCAGC 5 cut(s) 283, 656, 1120, 1255, 1557
BccI CCATC 3 cut(s) 93, 202, 713
BcgI CGANNNNNNTGC 6 cut(s) 93, 127, 633, 667, 1319, 1353
BciT130I CCWGG 4 cut(s) 215, 710, 927, 1494
BcnI CCSGG 2 cut(s) 131, 1382
BcoDI GTCTC 3 cut(s) 176, 377, 444
BcuI ACTAGT 1 cut(s) 1511
BfaI CTAG 2 cut(s) 365, 1512
BfmI CTRYAG 2 cut(s) 909, 1084
BfoI RGCGCY 1 cut(s) 423
BfrI CTTAAG 1 cut(s) 1280
BfuAI ACCTGC 2 cut(s) 395, 1376
BisI GCNGC 9 cut(s) 297, 423, 645, 714, 729, 943, 1134, 1269, 1571
BlpI GCTNAGC 1 cut(s) 416
BlsI GCNGC 9 cut(s) 298, 424, 646, 715, 730, 944, 1135, 1270, 1572
Bme1390I CCNGG 6 cut(s) 131, 215, 710, 927, 1382, 1494
Bme18I GGWCC 6 cut(s) 211, 303, 334, 702, 1063, 1457
BmeT110I CYCGRG 2 cut(s) 224, 1338
BmgBI CACGTC 1 cut(s) 1596
BmiI GGNNCC 4 cut(s) 135, 457, 1108, 1184
BmrFI CCNGG 6 cut(s) 131, 215, 710, 927, 1382, 1494
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 3 cut(s) 391, 954, 1317
BmuI ACTGGG 1 cut(s) 603
BpiI GAAGAC 1 cut(s) 1339
BpmI CTGGAG 1 cut(s) 581
Bpu1102I GCTNAGC 1 cut(s) 416
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 2 cut(s) 862, 1242
BpuMI CCSGG 2 cut(s) 131, 1382
BsaAI YACGTR 1 cut(s) 1165
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 4 cut(s) 459, 940, 1039, 1322
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 673, 1181, 1285, 1479, 1580
Bse118I RCCGGY 2 cut(s) 1285, 1317
Bse1I ACTGG 3 cut(s) 152, 609, 1585
Bse3DI GCAATG 1 cut(s) 933
BseBI CCWGG 4 cut(s) 215, 710, 927, 1494
BseDI CCNNGG 4 cut(s) 459, 940, 1039, 1322
BseGI GGATG 5 cut(s) 44, 85, 156, 705, 1332
BseLI CCNNNNNNNGG 6 cut(s) 130, 673, 1181, 1285, 1479, 1580
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 2 cut(s) 250, 430
BseNI ACTGG 3 cut(s) 152, 609, 1585
BseXI GCAGC 5 cut(s) 283, 656, 1120, 1255, 1557
BsgI GTGCAG 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 942
Bsh1285I CGRYCG 1 cut(s) 1565
BshFI GGCC 9 cut(s) 363, 693, 925, 992, 1185, 1317, 1321, 1492, 1602
BshNI GGYRCC 1 cut(s) 1106
BsiEI CGRYCG 1 cut(s) 1565
BsiHKAI GWGCWC 1 cut(s) 973
BsiHKCI CYCGRG 2 cut(s) 224, 1338
BsiSI CCGG 6 cut(s) 131, 966, 1286, 1318, 1382, 1557
BslFI GGGAC 2 cut(s) 139, 993
BslI CCNNNNNNNGG 6 cut(s) 130, 673, 1181, 1285, 1479, 1580
BsmAI GTCTC 3 cut(s) 176, 377, 444
BsmBI CGTCTC 2 cut(s) 176, 444
BsmFI GGGAC 2 cut(s) 139, 993
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 9 cut(s) 363, 693, 925, 992, 1185, 1317, 1321, 1492, 1602
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 2 cut(s) 224, 1338
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 2 cut(s) 235, 973
Bsp143I GATC 6 cut(s) 283, 455, 654, 686, 998, 1562
Bsp1720I GCTNAGC 1 cut(s) 416
Bsp19I CCATGG 1 cut(s) 1322
BspACI CCGC 7 cut(s) 349, 423, 713, 728, 940, 942, 986
BspANI GGCC 9 cut(s) 363, 693, 925, 992, 1185, 1317, 1321, 1492, 1602
BspCNI CTCAG 2 cut(s) 249, 429
BspFNI CGCG 1 cut(s) 942
BspHI TCATGA 1 cut(s) 1350
BspLI GGNNCC 4 cut(s) 135, 457, 1108, 1184
BspMAI CTGCAG 1 cut(s) 913
BspMI ACCTGC 2 cut(s) 395, 1376
BspPI GGATC 5 cut(s) 278, 450, 463, 694, 993
BspQI GCTCTTC 1 cut(s) 326
BspT104I TTCGAA 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 1106
BspTI CTTAAG 1 cut(s) 1280
BspTNI GGTCTC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 933
BsrFI RCCGGY 2 cut(s) 1285, 1317
BsrI ACTGG 3 cut(s) 152, 609, 1585
BssAI RCCGGY 2 cut(s) 1285, 1317
BssECI CCNNGG 4 cut(s) 459, 940, 1039, 1322
BssMI GATC 6 cut(s) 283, 455, 654, 686, 998, 1562
BssNAI GTATAC 1 cut(s) 849
BssSI CACGAG 1 cut(s) 668
BssT1I CCWWGG 3 cut(s) 459, 1039, 1322
Bst1107I GTATAC 1 cut(s) 849
Bst2BI CACGAG 1 cut(s) 668
Bst2UI CCWGG 4 cut(s) 215, 710, 927, 1494
Bst4CI ACNGT 5 cut(s) 170, 334, 536, 1028, 1540
Bst6I CTCTTC 1 cut(s) 326
BstAFI CTTAAG 1 cut(s) 1280
BstBAI YACGTR 1 cut(s) 1165
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 3 cut(s) 913, 940, 1319
BstDEI CTNAG 3 cut(s) 236, 416, 1421
BstDSI CCRYGG 2 cut(s) 940, 1322
BstF5I GGATG 5 cut(s) 44, 85, 156, 705, 1332
BstFNI CGCG 1 cut(s) 942
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 2 cut(s) 422, 1034
BstKTI GATC 6 cut(s) 286, 458, 657, 689, 1001, 1565
BstMAI GTCTC 3 cut(s) 176, 377, 444
BstMBI GATC 6 cut(s) 283, 455, 654, 686, 998, 1562
BstMCI CGRYCG 1 cut(s) 1565
BstMWI GCNNNNNNNGC 4 cut(s) 239, 1327, 1399, 1523
BstNI CCWGG 4 cut(s) 215, 710, 927, 1494
BstSCI CCNGG 6 cut(s) 129, 213, 708, 925, 1380, 1492
BstSFI CTRYAG 2 cut(s) 909, 1084
BstSNI TACGTA 1 cut(s) 1165
BstUI CGCG 1 cut(s) 942
BstV1I GCAGC 5 cut(s) 283, 656, 1120, 1255, 1557
BstV2I GAAGAC 1 cut(s) 1339
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BstZ17I GTATAC 1 cut(s) 849
BsuRI GGCC 9 cut(s) 363, 693, 925, 992, 1185, 1317, 1321, 1492, 1602
BtgI CCRYGG 2 cut(s) 940, 1322
BtrI CACGTC 1 cut(s) 1596
BtsCI GGATG 5 cut(s) 44, 85, 156, 705, 1332
BtsI GCAGTG 1 cut(s) 960
BtsIMutI CAGTG 3 cut(s) 244, 960, 1578
BveI ACCTGC 2 cut(s) 395, 1376
Cac8I GCNNGC 3 cut(s) 913, 940, 1319
CciI TCATGA 1 cut(s) 1350
CfoI GCGC 2 cut(s) 422, 1034
Cfr10I RCCGGY 2 cut(s) 1285, 1317
Cfr42I CCGCGG 1 cut(s) 943
Csp6I GTAC 1 cut(s) 1444
CviAII CATG 9 cut(s) 49, 108, 138, 764, 823, 1060, 1323, 1351, 1502
CviQI GTAC 1 cut(s) 1444
DdeI CTNAG 3 cut(s) 236, 416, 1421
DpnI GATC 6 cut(s) 285, 457, 656, 688, 1000, 1564
DpnII GATC 6 cut(s) 283, 455, 654, 686, 998, 1562
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 1 cut(s) 1319
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
Eco105I TACGTA 1 cut(s) 1165
Eco130I CCWWGG 3 cut(s) 459, 1039, 1322
Eco147I AGGCCT 2 cut(s) 363, 1602
Eco24I GRGCYC 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 6 cut(s) 211, 303, 334, 702, 1063, 1457
Eco88I CYCGRG 2 cut(s) 224, 1338
EcoRII CCWGG 4 cut(s) 213, 708, 925, 1492
EcoT14I CCWWGG 3 cut(s) 459, 1039, 1322
EcoT38I GRGCYC 1 cut(s) 235
ErhI CCWWGG 3 cut(s) 459, 1039, 1322
Esp3I CGTCTC 2 cut(s) 176, 444
FaeI CATG 9 cut(s) 52, 111, 141, 767, 826, 1063, 1326, 1354, 1505
FaqI GGGAC 2 cut(s) 139, 993
FatI CATG 9 cut(s) 48, 107, 137, 763, 822, 1059, 1322, 1350, 1501
FauI CCCGC 1 cut(s) 947
FblI GTMKAC 2 cut(s) 848, 1377
Fnu4HI GCNGC 9 cut(s) 297, 423, 645, 714, 729, 943, 1134, 1269, 1571
FokI GGATG 5 cut(s) 31, 72, 163, 692, 1339
FriOI GRGCYC 1 cut(s) 235
FseI GGCCGGCC 1 cut(s) 1321
Fsp4HI GCNGC 9 cut(s) 297, 423, 645, 714, 729, 943, 1134, 1269, 1571
FspBI CTAG 2 cut(s) 365, 1512
GlaI GCGC 2 cut(s) 421, 1033
GluI GCNGC 9 cut(s) 297, 423, 645, 714, 729, 943, 1134, 1269, 1571
GsuI CTGGAG 1 cut(s) 581
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 9 cut(s) 363, 693, 925, 992, 1185, 1317, 1321, 1492, 1602
HapII CCGG 6 cut(s) 131, 966, 1286, 1318, 1382, 1557
HhaI GCGC 2 cut(s) 422, 1034
Hin1II CATG 9 cut(s) 52, 111, 141, 767, 826, 1063, 1326, 1354, 1505
Hin6I GCGC 2 cut(s) 420, 1032
HinP1I GCGC 2 cut(s) 420, 1032
HincII GTYRAC 2 cut(s) 805, 1378
HindII GTYRAC 2 cut(s) 805, 1378
HinfI GANTC 2 cut(s) 599, 1291
HpaII CCGG 6 cut(s) 131, 966, 1286, 1318, 1382, 1557
HphI GGTGA 6 cut(s) 119, 166, 421, 845, 1300, 1528
Hpy188I TCNGA 5 cut(s) 60, 200, 520, 702, 1009
Hpy188III TCNNGA 5 cut(s) 72, 226, 560, 1147, 1351
HpyAV CCTTC 3 cut(s) 584, 1004, 1045
HpyCH4III ACNGT 5 cut(s) 170, 334, 536, 1028, 1540
HpyCH4IV ACGT 4 cut(s) 471, 500, 1164, 1595
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 1327, 1399, 1523
HpyF3I CTNAG 3 cut(s) 236, 416, 1421
HpySE526I ACGT 4 cut(s) 471, 500, 1164, 1595
Hsp92II CATG 9 cut(s) 52, 111, 141, 767, 826, 1063, 1326, 1354, 1505
HspAI GCGC 2 cut(s) 420, 1032
KroI GCCGGC 1 cut(s) 1317
KroNI GCCGGC 1 cut(s) 1319
KspI CCGCGG 1 cut(s) 943
Kzo9I GATC 6 cut(s) 283, 455, 654, 686, 998, 1562
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 3 cut(s) 133, 968, 1010
Lsp1109I GCAGC 5 cut(s) 283, 656, 1120, 1255, 1557
LweI GCATC 3 cut(s) 391, 954, 1317
MaeI CTAG 2 cut(s) 365, 1512
MaeII ACGT 4 cut(s) 471, 500, 1164, 1595
MaeIII GTNAC 3 cut(s) 884, 1094, 1358
MalI GATC 6 cut(s) 285, 457, 656, 688, 1000, 1564
MboI GATC 6 cut(s) 283, 455, 654, 686, 998, 1562
MboII GAAGA 3 cut(s) 313, 642, 1339
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 2 cut(s) 235, 973
MluCI AATT 7 cut(s) 260, 394, 543, 721, 1079, 1411, 1450
MlyI GAGTC 1 cut(s) 608
MmeI TCCRAC 3 cut(s) 528, 1032, 1522
MroNI GCCGGC 1 cut(s) 1317
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 3 cut(s) 719, 897, 1281
MslI CAYNNNNRTG 1 cut(s) 1058
MspA1I CMGCKG 1 cut(s) 942
MspCI CTTAAG 1 cut(s) 1280
MspI CCGG 6 cut(s) 131, 966, 1286, 1318, 1382, 1557
MspR9I CCNGG 6 cut(s) 131, 215, 710, 927, 1382, 1494
MunI CAATTG 1 cut(s) 543
Mva1269I GAATGC 1 cut(s) 99
MvaI CCWGG 4 cut(s) 215, 710, 927, 1494
MvnI CGCG 1 cut(s) 942
MwoI GCNNNNNNNGC 4 cut(s) 239, 1327, 1399, 1523
NaeI GCCGGC 1 cut(s) 1319
NciI CCSGG 2 cut(s) 131, 1382
NcoI CCATGG 1 cut(s) 1322
NdeII GATC 6 cut(s) 283, 455, 654, 686, 998, 1562
NgoMIV GCCGGC 1 cut(s) 1317
NlaIII CATG 9 cut(s) 52, 111, 141, 767, 826, 1063, 1326, 1354, 1505
NlaIV GGNNCC 4 cut(s) 135, 457, 1108, 1184
NmeAIII GCCGAG 1 cut(s) 473
NspV TTCGAA 1 cut(s) 103
PaeR7I CTCGAG 1 cut(s) 224
PagI TCATGA 1 cut(s) 1350
PceI AGGCCT 2 cut(s) 363, 1602
PciSI GCTCTTC 1 cut(s) 326
PctI GAATGC 1 cut(s) 99
PdiI GCCGGC 1 cut(s) 1319
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 1 cut(s) 1291
PkrI GCNGC 9 cut(s) 298, 424, 646, 715, 730, 944, 1135, 1270, 1572
Ple19I CGATCG 1 cut(s) 1565
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
Ppu21I YACGTR 1 cut(s) 1165
Psp6I CCWGG 4 cut(s) 213, 708, 925, 1492
PspGI CCWGG 4 cut(s) 213, 708, 925, 1492
PspN4I GGNNCC 4 cut(s) 135, 457, 1108, 1184
PstI CTGCAG 1 cut(s) 913
PsuI RGATCY 1 cut(s) 455
PvuI CGATCG 1 cut(s) 1565
RigI GGCCGGCC 1 cut(s) 1321
RsaI GTAC 1 cut(s) 1445
RsaNI GTAC 1 cut(s) 1444
RseI CAYNNNNRTG 1 cut(s) 1058
SacII CCGCGG 1 cut(s) 943
SalI GTCGAC 1 cut(s) 1376
SapI GCTCTTC 1 cut(s) 326
SaqAI TTAA 3 cut(s) 719, 897, 1281
SatI GCNGC 9 cut(s) 297, 423, 645, 714, 729, 943, 1134, 1269, 1571
Sau3AI GATC 6 cut(s) 283, 455, 654, 686, 998, 1562
SchI GAGTC 1 cut(s) 608
ScrFI CCNGG 6 cut(s) 131, 215, 710, 927, 1382, 1494
SduI GDGCHC 2 cut(s) 235, 973
SfaNI GCATC 3 cut(s) 391, 954, 1317
SfcI CTRYAG 2 cut(s) 909, 1084
Sfr274I CTCGAG 1 cut(s) 224
Sfr303I CCGCGG 1 cut(s) 943
SfuI TTCGAA 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 943
SinI GGWCC 6 cut(s) 211, 303, 334, 702, 1063, 1457
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 1058
SmlI CTYRAG 4 cut(s) 224, 841, 1257, 1280
SmoI CTYRAG 4 cut(s) 224, 841, 1257, 1280
SnaBI TACGTA 1 cut(s) 1165
SpeI ACTAGT 1 cut(s) 1511
Sse9I AATT 7 cut(s) 260, 394, 543, 721, 1079, 1411, 1450
SseBI AGGCCT 2 cut(s) 363, 1602
SsiI CCGC 7 cut(s) 349, 423, 713, 728, 940, 942, 986
SspI AATATT 1 cut(s) 1334
SspMI CTAG 2 cut(s) 365, 1512
StuI AGGCCT 2 cut(s) 363, 1602
StyD4I CCNGG 6 cut(s) 129, 213, 708, 925, 1380, 1492
StyI CCWWGG 3 cut(s) 459, 1039, 1322
TaaI ACNGT 5 cut(s) 170, 334, 536, 1028, 1540
TaiI ACGT 4 cut(s) 474, 503, 1167, 1598
TaqI TCGA 7 cut(s) 103, 225, 324, 476, 653, 997, 1377
TaqII GACCGA 1 cut(s) 320
TasI AATT 7 cut(s) 260, 394, 543, 721, 1079, 1411, 1450
TatI WGTACW 1 cut(s) 1443
TauI GCSGC 4 cut(s) 425, 716, 731, 945
TfiI GAWTC 1 cut(s) 1291
Tru1I TTAA 3 cut(s) 719, 897, 1281
Tru9I TTAA 3 cut(s) 719, 897, 1281
TscAI CASTG 3 cut(s) 244, 967, 1585
TseI GCWGC 5 cut(s) 296, 644, 1133, 1268, 1570
TspDTI ATGAA 6 cut(s) 23, 472, 922, 1339, 1367, 1490
TspGWI ACGGA 3 cut(s) 468, 699, 1578
TspRI CASTG 3 cut(s) 244, 967, 1585
Vha464I CTTAAG 1 cut(s) 1280
VpaK11BI GGWCC 6 cut(s) 211, 303, 334, 702, 1063, 1457
XapI RAATTY 2 cut(s) 721, 1450
XcmI CCANNNNNNNNNTGG 1 cut(s) 144
XhoI CTCGAG 1 cut(s) 224
XmiI GTMKAC 2 cut(s) 848, 1377
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 2 cut(s) 365, 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.