AT3G50390

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013946
Physical Location & Seq
Forward (+)
18702060 .. 18703731
1672 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50390.1

Sequence Viewer

Length: 1410 bp
ATGTCTTCAATGCATAAGAAAGGTACAAGCAGAGACGTAAATAGCCAAGCATTTACAAAACTAACGAAAGTAGAAACTATTCCAGATCCAGACATTTACGACATAACCTACGTAGATATCTCTCCCTCCGAAGGCTCCTCTTCACCATTATCCAAATCACCATGGTCCGTTCAAGTCGATCCATCCGCCGTAGATTCTCCGTATCATTCGGTGAAAGTTACGACGGATACAACCAAAGAAAAATCAACCAACCACTCTCCTAATATTCTCTTGGGGTCACTTGTTCGCGAAGAAGGTCATATCTATTCATTAGCTACATCAGGAGATTTACTCTACACAGGATCTGATTCCAAGAACATTAGGGTTTGGAAAAACCATGTTGAGTTCTCGAGTTTTAAATCGAATAGTGGATTGGTTAAAGCCATTGTTCTTGCTGGAGATAAAATCTTTACCGGTCATCAAGATGGTAAGATCCGTGTGTGGAAAGCTGCATCTAAAGAATCAAACGTTCATCGTCGTGTAGGTACAATGCCTAACCTTCTCGATTATATAAGAAACTCCATTGTTCCTTCTTCTTACTTCAACTTCACGCGACGTAATCGTAGCAGTGCTGCGTTAGGGTTTCGTCATTTAGACGCGATTTCTTGTCTTGCGTTAAGCGAAGATAAACGGTTGTTGTACTCTGGTTCTTGGGATAAAACGTTTAAGGTTTGGAGAGTTTCGGATTTGCGGTGTGTGGAATCTGTGAACGCTCATGAAGATGCGGTAAACGCTGTCGTTTCCGGTTTTGACGGTTTGGTTTTTACCGGTTCAGCTGATGGAACGGTTAAGGTTTGGAGAAGAGAAGATCAAGCGAAGGATACGAAACATTTCTTCTCTGAGACGTTATTGAAACAAGACTGTGCCGTTACGGCGATCGCTGTAGATCAAAGCGCGACGTTAGTTTATTGTGGTTCGTCTGACGGAACCGTTAATTTTTGGGAACGTGAGAATAATATGAAAAACGGCGGCGTTTTGAAAGGTCATAAACTCGCCGTGCTTTGTCTTGTCGCGGCGGGGAATTTGATGTTTAGTGGTTCTGCTGATTTAGGGATACGTGTGTGGAGGAGACCAGAAGGTGGAGGTGAGCATGTTTGTCTTTCGGTTTTGACCGGACATGCTGGTCCGGTGAAGTGTTTGGCGGTGGAGAGAGATCAAGAATCGGTTTCCGGTGAGAGACGGTGGATTGTGTATAGTGGGAGTTTAGATAGATCGGTGAAGATGTGGCGTGTGTCGGAGAGTTCACCGCCGATGGTGAATCAAGAGTTTAAGTTGCCGGATCAGTTTGGTGGTGGTGGTGGTGGTGGTCCGGCGGAGTTAACGGTGGCTCCTAGCTTCTCGGCTCAAGGGAGGATTAGTCCACAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

469

Amino Acids

51.2

Weight (kDa)

8.67

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 64 - 259 8.4e-06 SCAP Beta-propeller
WD40_WDHD1_1st PF24817 146 - 286 1.7e-11 WDHD1 first WD40 domain
WDR55 PF24796 208 - 280 6.2e-07 WDR55
Beta-prop_TEP1_2nd PF25047 209 - 285 3.1e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 209 - 282 8e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 210 - 238 4.4e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 210 - 280 7.6e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 210 - 282 2.3e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 210 - 266 7.1e-07 THOC3 beta-propeller domain
WD40_Gbeta PF25391 210 - 368 2.8e-14 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 211 - 402 8.6e-10 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 213 - 331 9.6e-15 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 214 - 331 7.1e-11 WDR3 second beta-propeller domain
NBCH_WD40 PF20426 215 - 321 4.5e-10 Neurobeachin beta propeller domain
Beta-prop_EML_2 PF23414 216 - 372 5.4e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 244 - 279 6.3e-06 WD domain, G-beta repeat
Beta-prop_SCAP PF24017 265 - 371 8.8e-06 SCAP Beta-propeller
Beta-prop_WDR3_2nd PF25172 266 - 371 1.6e-07 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 267 - 426 2.1e-17 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 285 - 433 2.3e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 295 - 426 7.7e-15 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 302 - 396 6.2e-09 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 310 - 436 9.2e-12 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50390
fragaria_vesca FvH4_1g14581
malus_domestica MD00G1067400.v1.1 MD02G1159400.v1.1
prunus_persica Prupe.7G144500_v2.0.a1
pyrus_communis pycom02g12570 pycom15g23710
rosa_chinensis RchiOBHm_Chr2g0103361
rosa_laevigata RLG00000017230
rosa_multiflora Rmu_sc0001555.1_g000004 Rmu_sc0002434.1_g000011
rosa_roxburghii Rroxscaffold_2G00139690
rosa_samantha Rh2AG162600 Rh2BG169700 Rh2CG169800 Rh2DG168700
rosa_wichuraiana Rw2G012770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1161
AccB7I CCANNNNNTGG 1 cut(s) 1118
AccII CGCG 5 cut(s) 288, 592, 638, 935, 1052
AciI CCGC 9 cut(s) 186, 730, 764, 1008, 1052, 1055, 1181, 1286, 1352
AclI AACGTT 2 cut(s) 507, 701
AclWI GGATC 5 cut(s) 80, 173, 349, 466, 1326
AcsI RAATTY 1 cut(s) 1060
AfaI GTAC 3 cut(s) 25, 526, 680
AfiI CCNNNNNNNGG 2 cut(s) 131, 1118
AflIII ACRYGT 1 cut(s) 1096
AgeI ACCGGT 2 cut(s) 452, 806
AgsI TTSAA 5 cut(s) 9, 173, 583, 892, 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AluBI AGCT 4 cut(s) 314, 488, 815, 1374
AluI AGCT 4 cut(s) 314, 488, 815, 1374
Alw26I GTCTC 4 cut(s) 27, 875, 1102, 1210
AlwI GGATC 5 cut(s) 80, 173, 349, 466, 1326
AlwNI CAGNNNCTG 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 388
ApeKI GCWGC 2 cut(s) 488, 611
ApoI RAATTY 1 cut(s) 1060
AsiGI ACCGGT 2 cut(s) 452, 806
AsiSI GCGATCGC 1 cut(s) 918
Asp700I GAANNNNTTC 2 cut(s) 78, 869
AspLEI GCGC 1 cut(s) 935
AspS9I GGNCC 3 cut(s) 165, 1163, 1346
AsuHPI GGTGA 9 cut(s) 135, 150, 223, 1136, 1180, 1223, 1267, 1275, 1306
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 3 cut(s) 165, 1163, 1346
BaeI ACNNNNGTAYC 2 cut(s) 1085, 1118
BbvI GCAGC 2 cut(s) 475, 598
BccI CCATC 4 cut(s) 190, 458, 812, 1285
BceAI ACGGC 5 cut(s) 173, 890, 927, 1019, 1021
BciVI GTATCC 3 cut(s) 220, 853, 1086
BcoDI GTCTC 4 cut(s) 27, 875, 1102, 1210
BfaI CTAG 1 cut(s) 1371
BfmI CTRYAG 1 cut(s) 921
BfuI GTATCC 3 cut(s) 220, 853, 1086
BglI GCCNNNNNGGC 1 cut(s) 911
BisI GCNGC 4 cut(s) 489, 612, 1009, 1053
BlsI GCNGC 4 cut(s) 490, 613, 1010, 1054
Bme18I GGWCC 3 cut(s) 165, 1163, 1346
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 3 cut(s) 165, 1163, 1346
BmiI GGNNCC 3 cut(s) 136, 967, 1368
BmsI GCATC 2 cut(s) 500, 751
BplI GAGNNNNNCTC 2 cut(s) 315, 347
BpmI CTGGAG 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 1368
BsaAI YACGTR 2 cut(s) 112, 1097
BsaI GGTCTC 1 cut(s) 1102
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 6 cut(s) 452, 782, 806, 1151, 1165, 1208
BsaXI ACNNNNNCTCC 3 cut(s) 1351, 1381, 1381
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 1118
Bse118I RCCGGY 2 cut(s) 452, 806
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 1 cut(s) 182
BseLI CCNNNNNNNGG 2 cut(s) 131, 1118
BseMII CTCAG 1 cut(s) 870
BseRI GAGGAG 2 cut(s) 127, 1120
BseXI GCAGC 2 cut(s) 475, 598
Bsh1236I CGCG 5 cut(s) 288, 592, 638, 935, 1052
Bsh1285I CGRYCG 1 cut(s) 918
BshTI ACCGGT 2 cut(s) 452, 806
BsiEI CGRYCG 1 cut(s) 918
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 8 cut(s) 453, 783, 807, 1152, 1166, 1209, 1316, 1349
BslI CCNNNNNNNGG 2 cut(s) 131, 1118
BsmAI GTCTC 4 cut(s) 27, 875, 1102, 1210
BsmBI CGTCTC 3 cut(s) 27, 875, 1210
Bso31I GGTCTC 1 cut(s) 1102
BsoBI CYCGRG 1 cut(s) 388
Bsp19I CCATGG 1 cut(s) 161
Bsp68I TCGCGA 1 cut(s) 288
BspACI CCGC 9 cut(s) 186, 730, 764, 1008, 1052, 1055, 1181, 1286, 1352
BspCNI CTCAG 1 cut(s) 871
BspFNI CGCG 5 cut(s) 288, 592, 638, 935, 1052
BspHI TCATGA 1 cut(s) 754
BspLI GGNNCC 3 cut(s) 136, 967, 1368
BspPI GGATC 5 cut(s) 80, 173, 349, 466, 1326
BspTNI GGTCTC 1 cut(s) 1102
BsrFI RCCGGY 2 cut(s) 452, 806
BssAI RCCGGY 2 cut(s) 452, 806
BssECI CCNNGG 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 7 cut(s) 672, 794, 826, 902, 970, 1221, 1363
Bst6I CTCTTC 2 cut(s) 145, 835
BstBAI YACGTR 2 cut(s) 112, 1097
BstDEI CTNAG 1 cut(s) 879
BstDSI CCRYGG 1 cut(s) 161
BstF5I GGATG 1 cut(s) 182
BstFNI CGCG 5 cut(s) 288, 592, 638, 935, 1052
BstHHI GCGC 1 cut(s) 935
BstMAI GTCTC 4 cut(s) 27, 875, 1102, 1210
BstMCI CGRYCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 2 cut(s) 770, 911
BstNSI RCATGY 2 cut(s) 1133, 1160
BstSFI CTRYAG 1 cut(s) 921
BstSNI TACGTA 1 cut(s) 112
BstUI CGCG 5 cut(s) 288, 592, 638, 935, 1052
BstV1I GCAGC 2 cut(s) 475, 598
BstX2I RGATCY 3 cut(s) 85, 341, 471
BstYI RGATCY 3 cut(s) 85, 341, 471
BsuI GTATCC 3 cut(s) 220, 853, 1086
BtgI CCRYGG 1 cut(s) 161
BtsCI GGATG 1 cut(s) 182
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 1 cut(s) 613
BtuMI TCGCGA 1 cut(s) 288
CaiI CAGNNNCTG 1 cut(s) 344
CciI TCATGA 1 cut(s) 754
CfoI GCGC 1 cut(s) 935
Cfr10I RCCGGY 2 cut(s) 452, 806
Cfr13I GGNCC 3 cut(s) 165, 1163, 1346
CseI GACGC 1 cut(s) 644
Csp6I GTAC 3 cut(s) 24, 525, 679
CspAI ACCGGT 2 cut(s) 452, 806
CviAII CATG 5 cut(s) 162, 377, 755, 1130, 1157
CviJI RGCY 9 cut(s) 45, 135, 314, 422, 488, 815, 1367, 1374, 1382
CviKI_1 RGCY 9 cut(s) 45, 135, 314, 422, 488, 815, 1367, 1374, 1382
CviQI GTAC 3 cut(s) 24, 525, 679
DdeI CTNAG 1 cut(s) 879
DraI TTTAAA 1 cut(s) 397
DrdI GACNNNNNNGTC 1 cut(s) 1161
DseDI GACNNNNNNGTC 1 cut(s) 1161
Eam1104I CTCTTC 2 cut(s) 145, 835
EarI CTCTTC 2 cut(s) 145, 835
EciI GGCGGA 2 cut(s) 175, 1367
Eco105I TACGTA 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 161
Eco31I GGTCTC 1 cut(s) 1102
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 3 cut(s) 165, 1163, 1346
Eco88I CYCGRG 1 cut(s) 388
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 161
EcoT22I ATGCAT 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 161
Esp3I CGTCTC 3 cut(s) 27, 875, 1210
FaeI CATG 5 cut(s) 165, 380, 758, 1133, 1160
FatI CATG 5 cut(s) 161, 376, 754, 1129, 1156
FauI CCCGC 1 cut(s) 1048
Fnu4HI GCNGC 4 cut(s) 489, 612, 1009, 1053
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 4 cut(s) 489, 612, 1009, 1053
FspBI CTAG 1 cut(s) 1371
GlaI GCGC 1 cut(s) 934
GluI GCNGC 4 cut(s) 489, 612, 1009, 1053
GsuI CTGGAG 1 cut(s) 456
HapII CCGG 8 cut(s) 453, 783, 807, 1152, 1166, 1209, 1316, 1349
HgaI GACGC 1 cut(s) 644
HhaI GCGC 1 cut(s) 935
Hin1II CATG 5 cut(s) 165, 380, 758, 1133, 1160
Hin6I GCGC 1 cut(s) 933
HinP1I GCGC 1 cut(s) 933
HincII GTYRAC 1 cut(s) 1359
HindII GTYRAC 1 cut(s) 1359
HinfI GANTC 6 cut(s) 194, 347, 500, 740, 1199, 1297
HpaI GTTAAC 1 cut(s) 1359
HpaII CCGG 8 cut(s) 453, 783, 807, 1152, 1166, 1209, 1316, 1349
HphI GGTGA 9 cut(s) 135, 150, 223, 1136, 1180, 1223, 1267, 1275, 1306
Hpy166II GTNNAC 5 cut(s) 748, 769, 1283, 1359, 1400
Hpy188I TCNGA 6 cut(s) 130, 346, 724, 880, 961, 1276
Hpy8I GTNNAC 5 cut(s) 748, 769, 1283, 1359, 1400
Hpy99I CGWCG 4 cut(s) 226, 519, 597, 940
HpyAV CCTTC 6 cut(s) 125, 287, 548, 579, 850, 1109
HpyCH4III ACNGT 7 cut(s) 672, 794, 826, 902, 970, 1221, 1363
HpyCH4IV ACGT 9 cut(s) 36, 111, 507, 595, 701, 884, 938, 985, 1096
HpyCH4V TGCA 2 cut(s) 13, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 770, 911
HpyF3I CTNAG 1 cut(s) 879
HpySE526I ACGT 9 cut(s) 36, 111, 507, 595, 701, 884, 938, 985, 1096
Hsp92II CATG 5 cut(s) 165, 380, 758, 1133, 1160
HspAI GCGC 1 cut(s) 933
KspAI GTTAAC 1 cut(s) 1359
LmnI GCTCC 2 cut(s) 140, 1372
Lsp1109I GCAGC 2 cut(s) 475, 598
LweI GCATC 2 cut(s) 500, 751
MaeI CTAG 1 cut(s) 1371
MaeII ACGT 9 cut(s) 36, 111, 507, 595, 701, 884, 938, 985, 1096
MaeIII GTNAC 3 cut(s) 217, 276, 907
MboII GAAGA 9 cut(s) 132, 302, 564, 674, 770, 852, 857, 865, 1270
MflI RGATCY 3 cut(s) 85, 341, 471
MluCI AATT 2 cut(s) 973, 1060
MmeI TCCRAC 1 cut(s) 1254
MnlI CCTC 5 cut(s) 136, 148, 1098, 1115, 1383
Mph1103I ATGCAT 1 cut(s) 15
MroXI GAANNNNTTC 2 cut(s) 78, 869
MseI TTAA 8 cut(s) 396, 417, 656, 705, 828, 972, 1308, 1358
MslI CAYNNNNRTG 3 cut(s) 462, 516, 759
MspA1I CMGCKG 1 cut(s) 815
MspI CCGG 8 cut(s) 453, 783, 807, 1152, 1166, 1209, 1316, 1349
MvnI CGCG 5 cut(s) 288, 592, 638, 935, 1052
MwoI GCNNNNNNNGC 2 cut(s) 770, 911
NcoI CCATGG 1 cut(s) 161
NlaIII CATG 5 cut(s) 165, 380, 758, 1133, 1160
NlaIV GGNNCC 3 cut(s) 136, 967, 1368
NmeAIII GCCGAG 1 cut(s) 1358
NmuCI GTSAC 1 cut(s) 276
NruI TCGCGA 1 cut(s) 288
NsiI ATGCAT 1 cut(s) 15
NspI RCATGY 2 cut(s) 1133, 1160
PaeR7I CTCGAG 1 cut(s) 388
PagI TCATGA 1 cut(s) 754
PdmI GAANNNNTTC 2 cut(s) 78, 869
PfeI GAWTC 6 cut(s) 194, 347, 500, 740, 1199, 1297
PflMI CCANNNNNTGG 1 cut(s) 1118
PinAI ACCGGT 2 cut(s) 452, 806
PkrI GCNGC 4 cut(s) 490, 613, 1010, 1054
Ple19I CGATCG 1 cut(s) 918
Ppu21I YACGTR 2 cut(s) 112, 1097
Psp1406I AACGTT 2 cut(s) 507, 701
PspN4I GGNNCC 3 cut(s) 136, 967, 1368
PspPI GGNCC 3 cut(s) 165, 1163, 1346
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 3 cut(s) 85, 341, 471
PvuI CGATCG 1 cut(s) 918
PvuII CAGCTG 1 cut(s) 815
RgaI GCGATCGC 1 cut(s) 918
RruI TCGCGA 1 cut(s) 288
RsaI GTAC 3 cut(s) 25, 526, 680
RsaNI GTAC 3 cut(s) 24, 525, 679
RseI CAYNNNNRTG 3 cut(s) 462, 516, 759
SaqAI TTAA 8 cut(s) 396, 417, 656, 705, 828, 972, 1308, 1358
SatI GCNGC 4 cut(s) 489, 612, 1009, 1053
Sau96I GGNCC 3 cut(s) 165, 1163, 1346
SfaAI GCGATCGC 1 cut(s) 918
SfaNI GCATC 2 cut(s) 500, 751
SfcI CTRYAG 1 cut(s) 921
Sfr274I CTCGAG 1 cut(s) 388
SgfI GCGATCGC 1 cut(s) 918
SinI GGWCC 3 cut(s) 165, 1163, 1346
SlaI CTCGAG 1 cut(s) 388
SmiMI CAYNNNNRTG 3 cut(s) 462, 516, 759
SmlI CTYRAG 2 cut(s) 388, 1383
SmoI CTYRAG 2 cut(s) 388, 1383
SnaBI TACGTA 1 cut(s) 112
Sse9I AATT 2 cut(s) 973, 1060
SsiI CCGC 9 cut(s) 186, 730, 764, 1008, 1052, 1055, 1181, 1286, 1352
SspI AATATT 1 cut(s) 265
SspMI CTAG 1 cut(s) 1371
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 7 cut(s) 672, 794, 826, 902, 970, 1221, 1363
TaiI ACGT 9 cut(s) 39, 114, 510, 598, 704, 887, 941, 988, 1099
TaqI TCGA 4 cut(s) 177, 389, 401, 543
TasI AATT 2 cut(s) 973, 1060
TatI WGTACW 1 cut(s) 678
TauI GCSGC 2 cut(s) 1011, 1055
TfiI GAWTC 6 cut(s) 194, 347, 500, 740, 1199, 1297
Tru1I TTAA 8 cut(s) 396, 417, 656, 705, 828, 972, 1308, 1358
Tru9I TTAA 8 cut(s) 396, 417, 656, 705, 828, 972, 1308, 1358
TscAI CASTG 1 cut(s) 613
TseFI GTSAC 1 cut(s) 276
TseI GCWGC 2 cut(s) 488, 611
Tsp45I GTSAC 1 cut(s) 276
TspDTI ATGAA 4 cut(s) 297, 500, 771, 1013
TspGWI ACGGA 5 cut(s) 157, 189, 239, 464, 978
TspRI CASTG 1 cut(s) 613
Van91I CCANNNNNTGG 1 cut(s) 1118
VpaK11BI GGWCC 3 cut(s) 165, 1163, 1346
XapI RAATTY 1 cut(s) 1060
XceI RCATGY 2 cut(s) 1133, 1160
XhoI CTCGAG 1 cut(s) 388
XmnI GAANNNNTTC 2 cut(s) 78, 869
XspI CTAG 1 cut(s) 1371
Zsp2I ATGCAT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.