pycom02g12570

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013946
Physical Location & Seq
Forward (+)
9213289 .. 9214931
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12570.2

Sequence Viewer

Length: 1410 bp
ATGATTGGCCACTCGTCCAGAGACAACCGCATATTCTCCGAAGAGAACAGCATTCGTGGCCAAAAGTTCGACGGGTTAAAGAACTTCCCGGACCACGACAACTCGGGTTTTTCGATTCGATTCGATCCCCCAAGCTACGAGCCAGACTCGATGGTGGCGGAGGAGGCCGCTTTGTCTCCTCTGAATAAGTCGCCATGGTCTTCTCATATGAACAACAATATCAACAACGGCGATAATAATAATGCTTCAGATACGGCTAACGATAACTTCTCCCCCAACGTCCTCATAGGCGCGTTGGTGCGCGAGGAAGGTCACATATACTCACTGGCCGCCTCTGGAGACTTGTTGTACACGGGATCGGATAGCAAGAACATTCGGATTTGGAAGCAGCACAAAGAGTATTCGGGGTTCAAATCGAACAGTGGGTTGGTTAAAGCGATAATCATAGCGAGGGAGAAGATCTTCACCGGTCATCAAGACGGGAAAATTAGGGTTTGGAAGGTGTCCTCCAAGAACGCAAACCACAAGCGCATAGGAACTTTACCGACGTTGAAGAATTACATTAAGTGTTCCATGAAGCCGAGTAATTACGTTGAAGTGAGAGGCAAGAGCAAAGTCCTATGGATCAAGCATTTTGACTCCATCTCATGCTTGAGCTTGAGCGAAGATCAAACGTTGCTTTACTCTGCGTCGTGGGATAAAACGTTTAAGGTATGGAGGGTTTCCGATTTCAAGTGCTTGGAGTCGATCAACGCTCATGAAGACGCCGTGAATGCATTGGTTGTTGGATTTGATGGGTTGGTCTTCACCGCTTCCGCAGATGGAACGGTTAAGATTTGGCGGAAAGAGCTACAAGGAGAAGGTACTAAGCACTTCTTCTCACAAACGCTCTTAAAGCAGAAATACGCAATCACATCCCTAGCTGTGAACACAGACGCCTCGATCATATATTGTGGCTCGTCCGACGGGCTAGTCAACTTTTGGGAACGGGAGAAGAACCTCCAACACGGCGGGGTTTTGAGAGGCCACAAACTTGCTGTTCTGTGCTTGGCCACGGCCGGTTGCTTAGTATTCAGTGGCTCCGCCGATATGGGGATTTGCGTGTGGAGATTAGGCCCCGACGGGGATCACATTTGCCTGTCGGTGCTGTCGGGGCATACGGGGCCGGTTAAGTGCTTGGCTGTGGAGAGAGATCACGATCAGTCGACATCCGGGGAGAAGAGGTGGATCGTGTATAGTGGAAGCTTGGATAAGTCGGTGAAGATGTGGAGGGTTTCGGAACAAGCGCTGCCTATGGTGCCAAACCAGAAGCACCAGCACTACACGTCCGATGGGAATTGTGTGCCGATGTTGAGCTCGGCGCCTAGCTTTGCTTCTCGTGGAGGGGAAATGGGTTCAAGAAGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

470

Amino Acids

51.93

Weight (kDa)

8.65

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50390
fragaria_vesca FvH4_1g14581
malus_domestica MD00G1067400.v1.1 MD02G1159400.v1.1
prunus_persica Prupe.7G144500_v2.0.a1
pyrus_communis pycom02g12570 pycom15g23710
rosa_chinensis RchiOBHm_Chr2g0103361
rosa_laevigata RLG00000017230
rosa_multiflora Rmu_sc0001555.1_g000004 Rmu_sc0002434.1_g000011
rosa_roxburghii Rroxscaffold_2G00139690
rosa_samantha Rh2AG162600 Rh2BG169700 Rh2CG169800 Rh2DG168700
rosa_wichuraiana Rw2G012770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 971
AccB1I GGYRCC 2 cut(s) 1297, 1360
AccI GTMKAC 1 cut(s) 1205
AccII CGCG 2 cut(s) 293, 303
AciI CCGC 9 cut(s) 28, 158, 168, 330, 810, 816, 841, 1011, 1083
AclI AACGTT 2 cut(s) 674, 704
AclWI GGATC 5 cut(s) 119, 364, 632, 1134, 1235
AcoI YGGCCR 5 cut(s) 7, 58, 327, 1050, 1056
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 3 cut(s) 765, 936, 1361
AfaI GTAC 2 cut(s) 350, 865
AfeI AGCGCT 1 cut(s) 1287
AfiI CCNNNNNNNGG 2 cut(s) 1092, 1123
AflIII ACRYGT 1 cut(s) 1323
AgeI ACCGGT 1 cut(s) 467
AgsI TTSAA 5 cut(s) 412, 553, 596, 733, 1398
AjiI CACGTC 1 cut(s) 1326
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 7 cut(s) 135, 657, 850, 923, 1245, 1356, 1368
AluI AGCT 7 cut(s) 135, 657, 850, 923, 1245, 1356, 1368
Alw21I GWGCWC 1 cut(s) 1358
Alw26I GTCTC 3 cut(s) 15, 180, 333
AlwI GGATC 5 cut(s) 119, 364, 632, 1134, 1235
Ama87I CYCGRG 1 cut(s) 103
Aor51HI AGCGCT 1 cut(s) 1287
AoxI GGCC 9 cut(s) 7, 58, 165, 327, 1024, 1050, 1056, 1114, 1163
ApeKI GCWGC 2 cut(s) 388, 1288
AsiGI ACCGGT 1 cut(s) 467
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 5 cut(s) 293, 303, 531, 1288, 1363
AspS9I GGNCC 3 cut(s) 91, 1115, 1163
AsuC2I CCSGG 2 cut(s) 89, 1213
AsuHPI GGTGA 3 cut(s) 457, 799, 1270
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 1 cut(s) 91
BalI TGGCCA 3 cut(s) 9, 60, 1052
BanI GGYRCC 2 cut(s) 1297, 1360
BanII GRGCYC 1 cut(s) 1358
BauI CACGAG 1 cut(s) 1377
BbsI GAAGAC 3 cut(s) 192, 768, 796
Bbv12I GWGCWC 1 cut(s) 1358
BbvI GCAGC 2 cut(s) 400, 1275
BccI CCATC 5 cut(s) 145, 650, 788, 815, 1325
BceAI ACGGC 5 cut(s) 244, 270, 752, 1024, 1071
BcnI CCSGG 2 cut(s) 89, 1213
BcoDI GTCTC 3 cut(s) 15, 180, 333
BfaI CTAG 3 cut(s) 920, 971, 1365
BfoI RGCGCY 2 cut(s) 1289, 1364
BglII AGATCT 1 cut(s) 459
BisI GCNGC 4 cut(s) 168, 330, 389, 1289
BlsI GCNGC 4 cut(s) 169, 331, 390, 1290
Bme1390I CCNGG 2 cut(s) 89, 1213
Bme18I GGWCC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 103
BmgBI CACGTC 1 cut(s) 1326
BmgT120I GGNCC 3 cut(s) 91, 1115, 1163
BmiI GGNNCC 5 cut(s) 1081, 1117, 1164, 1299, 1362
BmrFI CCNGG 2 cut(s) 89, 1213
BpiI GAAGAC 3 cut(s) 192, 768, 796
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 1 cut(s) 357
BpuEI CTTGAG 2 cut(s) 673, 679
BpuMI CCSGG 2 cut(s) 89, 1213
BsaBI GATNNNNATC 1 cut(s) 1197
BsaHI GRCGYC 3 cut(s) 765, 936, 1361
BsaJI CCNNGG 3 cut(s) 194, 1053, 1212
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 1208, 1238
Bsc4I CCNNNNNNNGG 2 cut(s) 1092, 1123
Bse118I RCCGGY 3 cut(s) 467, 1058, 1165
Bse1I ACTGG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 1197
BseDI CCNNGG 3 cut(s) 194, 1053, 1212
BseGI GGATG 2 cut(s) 914, 1208
BseJI GATNNNNATC 1 cut(s) 1197
BseLI CCNNNNNNNGG 2 cut(s) 1092, 1123
BseNI ACTGG 1 cut(s) 330
BseRI GAGGAG 2 cut(s) 168, 176
BseX3I CGGCCG 1 cut(s) 1056
BseXI GCAGC 2 cut(s) 400, 1275
Bsh1236I CGCG 2 cut(s) 293, 303
Bsh1285I CGRYCG 1 cut(s) 1059
BshFI GGCC 9 cut(s) 9, 60, 167, 329, 1026, 1052, 1058, 1116, 1165
BshNI GGYRCC 2 cut(s) 1297, 1360
BshTI ACCGGT 1 cut(s) 467
BsiEI CGRYCG 1 cut(s) 1059
BsiHKAI GWGCWC 1 cut(s) 1358
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 5 cut(s) 89, 468, 1059, 1166, 1212
BslI CCNNNNNNNGG 2 cut(s) 1092, 1123
BsmAI GTCTC 3 cut(s) 15, 180, 333
BsmI GAATGC 2 cut(s) 51, 778
BsnI GGCC 9 cut(s) 9, 60, 167, 329, 1026, 1052, 1058, 1116, 1165
BsoBI CYCGRG 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 1358
Bsp1407I TGTACA 1 cut(s) 348
Bsp19I CCATGG 1 cut(s) 194
BspACI CCGC 9 cut(s) 28, 158, 168, 330, 810, 816, 841, 1011, 1083
BspANI GGCC 9 cut(s) 9, 60, 167, 329, 1026, 1052, 1058, 1116, 1165
BspFNI CGCG 2 cut(s) 293, 303
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 5 cut(s) 1081, 1117, 1164, 1299, 1362
BspPI GGATC 5 cut(s) 119, 364, 632, 1134, 1235
BspT107I GGYRCC 2 cut(s) 1297, 1360
BsrFI RCCGGY 3 cut(s) 467, 1058, 1165
BsrGI TGTACA 1 cut(s) 348
BsrI ACTGG 1 cut(s) 330
BssAI RCCGGY 3 cut(s) 467, 1058, 1165
BssECI CCNNGG 3 cut(s) 194, 1053, 1212
BssNI GRCGYC 3 cut(s) 765, 936, 1361
BssSI CACGAG 1 cut(s) 1377
BssT1I CCWWGG 1 cut(s) 194
Bst2BI CACGAG 1 cut(s) 1377
Bst4CI ACNGT 2 cut(s) 422, 829
Bst6I CTCTTC 2 cut(s) 36, 1214
BstACI GRCGYC 3 cut(s) 765, 936, 1361
BstAUI TGTACA 1 cut(s) 348
BstDEI CTNAG 2 cut(s) 867, 1066
BstDSI CCRYGG 2 cut(s) 194, 1053
BstF5I GGATG 2 cut(s) 914, 1208
BstFNI CGCG 2 cut(s) 293, 303
BstH2I RGCGCY 2 cut(s) 1289, 1364
BstHHI GCGC 5 cut(s) 293, 303, 531, 1288, 1363
BstMAI GTCTC 3 cut(s) 15, 180, 333
BstMCI CGRYCG 1 cut(s) 1059
BstMWI GCNNNNNNNGC 8 cut(s) 57, 164, 773, 847, 895, 1153, 1162, 1297
BstSCI CCNGG 2 cut(s) 87, 1211
BstUI CGCG 2 cut(s) 293, 303
BstV1I GCAGC 2 cut(s) 400, 1275
BstV2I GAAGAC 3 cut(s) 192, 768, 796
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BstZI CGGCCG 1 cut(s) 1056
BsuRI GGCC 9 cut(s) 9, 60, 167, 329, 1026, 1052, 1058, 1116, 1165
BtgI CCRYGG 2 cut(s) 194, 1053
BtrI CACGTC 1 cut(s) 1326
BtsCI GGATG 2 cut(s) 914, 1208
BtsIMutI CAGTG 3 cut(s) 323, 427, 1081
CciI TCATGA 1 cut(s) 757
CfoI GCGC 5 cut(s) 293, 303, 531, 1288, 1363
Cfr10I RCCGGY 3 cut(s) 467, 1058, 1165
Cfr13I GGNCC 3 cut(s) 91, 1115, 1163
CseI GACGC 3 cut(s) 678, 773, 944
Csp6I GTAC 2 cut(s) 349, 864
CspAI ACCGGT 1 cut(s) 467
CviAII CATG 4 cut(s) 195, 574, 648, 758
CviQI GTAC 2 cut(s) 349, 864
DdeI CTNAG 2 cut(s) 867, 1066
DinI GGCGCC 1 cut(s) 1362
DrdI GACNNNNNNGTC 1 cut(s) 971
DseDI GACNNNNNNGTC 1 cut(s) 971
EaeI YGGCCR 5 cut(s) 7, 58, 327, 1050, 1056
EagI CGGCCG 1 cut(s) 1056
Eam1104I CTCTTC 2 cut(s) 36, 1214
EarI CTCTTC 2 cut(s) 36, 1214
EciI GGCGGA 3 cut(s) 173, 856, 1072
Ecl136II GAGCTC 1 cut(s) 1356
EclXI CGGCCG 1 cut(s) 1056
Eco130I CCWWGG 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 1358
Eco47I GGWCC 1 cut(s) 91
Eco47III AGCGCT 1 cut(s) 1287
Eco52I CGGCCG 1 cut(s) 1056
Eco53kI GAGCTC 1 cut(s) 1356
Eco57I CTGAAG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 1356
EcoO109I RGGNCCY 1 cut(s) 1115
EcoT14I CCWWGG 1 cut(s) 194
EcoT22I ATGCAT 1 cut(s) 778
EcoT38I GRGCYC 1 cut(s) 1358
EgeI GGCGCC 1 cut(s) 1362
EheI GGCGCC 1 cut(s) 1362
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 4 cut(s) 198, 577, 651, 761
FalI AAGNNNNNCTT 2 cut(s) 860, 892
FatI CATG 4 cut(s) 194, 573, 647, 757
FauI CCCGC 1 cut(s) 1004
FauNDI CATATG 1 cut(s) 207
FblI GTMKAC 1 cut(s) 1205
Fnu4HI GCNGC 4 cut(s) 168, 330, 389, 1289
FokI GGATG 2 cut(s) 901, 1195
FriOI GRGCYC 1 cut(s) 1358
Fsp4HI GCNGC 4 cut(s) 168, 330, 389, 1289
FspBI CTAG 3 cut(s) 920, 971, 1365
GlaI GCGC 5 cut(s) 292, 302, 530, 1287, 1362
GluI GCNGC 4 cut(s) 168, 330, 389, 1289
GsuI CTGGAG 1 cut(s) 357
HaeII RGCGCY 2 cut(s) 1289, 1364
HaeIII GGCC 9 cut(s) 9, 60, 167, 329, 1026, 1052, 1058, 1116, 1165
HapII CCGG 5 cut(s) 89, 468, 1059, 1166, 1212
HgaI GACGC 3 cut(s) 678, 773, 944
HhaI GCGC 5 cut(s) 293, 303, 531, 1288, 1363
Hin1I GRCGYC 3 cut(s) 765, 936, 1361
Hin1II CATG 4 cut(s) 198, 577, 651, 761
Hin6I GCGC 5 cut(s) 291, 301, 529, 1286, 1361
HinP1I GCGC 5 cut(s) 291, 301, 529, 1286, 1361
HincII GTYRAC 2 cut(s) 976, 1206
HindII GTYRAC 2 cut(s) 976, 1206
HindIII AAGCTT 1 cut(s) 1243
HinfI GANTC 5 cut(s) 115, 120, 146, 638, 743
HpaII CCGG 5 cut(s) 89, 468, 1059, 1166, 1212
HphI GGTGA 3 cut(s) 457, 799, 1270
Hpy166II GTNNAC 4 cut(s) 351, 928, 976, 1206
Hpy188I TCNGA 9 cut(s) 40, 183, 250, 361, 378, 727, 964, 1279, 1330
Hpy188III TCNNGA 6 cut(s) 18, 336, 476, 758, 1196, 1398
Hpy8I GTNNAC 4 cut(s) 351, 928, 976, 1206
Hpy99I CGWCG 5 cut(s) 74, 550, 694, 968, 1124
HpyAV CCTTC 3 cut(s) 302, 493, 854
HpyCH4III ACNGT 2 cut(s) 422, 829
HpyCH4IV ACGT 6 cut(s) 279, 548, 591, 674, 704, 1325
HpyCH4V TGCA 1 cut(s) 776
HpyF10VI GCNNNNNNNGC 8 cut(s) 57, 164, 773, 847, 895, 1153, 1162, 1297
HpyF3I CTNAG 2 cut(s) 867, 1066
HpySE526I ACGT 6 cut(s) 279, 548, 591, 674, 704, 1325
Hsp92I GRCGYC 3 cut(s) 765, 936, 1361
Hsp92II CATG 4 cut(s) 198, 577, 651, 761
HspAI GCGC 5 cut(s) 291, 301, 529, 1286, 1361
KasI GGCGCC 1 cut(s) 1360
LmnI GCTCC 1 cut(s) 1085
Lsp1109I GCAGC 2 cut(s) 400, 1275
MaeI CTAG 3 cut(s) 920, 971, 1365
MaeII ACGT 6 cut(s) 279, 548, 591, 674, 704, 1325
MaeIII GTNAC 1 cut(s) 311
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 1358
MlsI TGGCCA 3 cut(s) 9, 60, 1052
MluCI AATT 4 cut(s) 486, 556, 586, 1336
MluNI TGGCCA 3 cut(s) 9, 60, 1052
Mly113I GGCGCC 1 cut(s) 1361
MlyI GAGTC 3 cut(s) 140, 632, 752
MmeI TCCRAC 3 cut(s) 766, 987, 1027
Mox20I TGGCCA 3 cut(s) 9, 60, 1052
Mph1103I ATGCAT 1 cut(s) 778
MroXI GAANNNNTTC 1 cut(s) 461
MscI TGGCCA 3 cut(s) 9, 60, 1052
MseI TTAA 7 cut(s) 77, 432, 564, 708, 831, 893, 1170
Msp20I TGGCCA 3 cut(s) 9, 60, 1052
MspI CCGG 5 cut(s) 89, 468, 1059, 1166, 1212
MspR9I CCNGG 2 cut(s) 89, 1213
Mva1269I GAATGC 2 cut(s) 51, 778
MvnI CGCG 2 cut(s) 293, 303
MwoI GCNNNNNNNGC 8 cut(s) 57, 164, 773, 847, 895, 1153, 1162, 1297
NarI GGCGCC 1 cut(s) 1361
NciI CCSGG 2 cut(s) 89, 1213
NcoI CCATGG 1 cut(s) 194
NdeI CATATG 1 cut(s) 207
NlaIII CATG 4 cut(s) 198, 577, 651, 761
NlaIV GGNNCC 5 cut(s) 1081, 1117, 1164, 1299, 1362
NmeAIII GCCGAG 2 cut(s) 606, 1337
NmuCI GTSAC 1 cut(s) 311
NsiI ATGCAT 1 cut(s) 778
PagI TCATGA 1 cut(s) 757
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PctI GAATGC 2 cut(s) 51, 778
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 2 cut(s) 115, 120
PfoI TCCNGGA 1 cut(s) 87
PinAI ACCGGT 1 cut(s) 467
PkrI GCNGC 4 cut(s) 169, 331, 390, 1290
PleI GAGTC 3 cut(s) 140, 632, 751
PluTI GGCGCC 1 cut(s) 1364
PpsI GAGTC 3 cut(s) 140, 632, 751
Psp124BI GAGCTC 1 cut(s) 1358
Psp1406I AACGTT 2 cut(s) 674, 704
PspN4I GGNNCC 5 cut(s) 1081, 1117, 1164, 1299, 1362
PspPI GGNCC 3 cut(s) 91, 1115, 1163
PsrI GAACNNNNNNTAC 2 cut(s) 392, 424
PsuI RGATCY 1 cut(s) 459
RsaI GTAC 2 cut(s) 350, 865
RsaNI GTAC 2 cut(s) 349, 864
SacI GAGCTC 1 cut(s) 1358
SalI GTCGAC 1 cut(s) 1204
SaqAI TTAA 7 cut(s) 77, 432, 564, 708, 831, 893, 1170
SatI GCNGC 4 cut(s) 168, 330, 389, 1289
Sau96I GGNCC 3 cut(s) 91, 1115, 1163
SchI GAGTC 3 cut(s) 140, 632, 752
ScrFI CCNGG 2 cut(s) 89, 1213
SduI GDGCHC 1 cut(s) 1358
SfoI GGCGCC 1 cut(s) 1362
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 2 cut(s) 652, 658
SmoI CTYRAG 2 cut(s) 652, 658
Sse9I AATT 4 cut(s) 486, 556, 586, 1336
SsiI CCGC 9 cut(s) 28, 158, 168, 330, 810, 816, 841, 1011, 1083
SspDI GGCGCC 1 cut(s) 1360
SspMI CTAG 3 cut(s) 920, 971, 1365
SstI GAGCTC 1 cut(s) 1358
StyD4I CCNGG 2 cut(s) 87, 1211
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 2 cut(s) 422, 829
TaiI ACGT 6 cut(s) 282, 551, 594, 677, 707, 1328
TaqI TCGA 9 cut(s) 69, 113, 118, 123, 149, 416, 746, 941, 1205
TasI AATT 4 cut(s) 486, 556, 586, 1336
TatI WGTACW 1 cut(s) 348
TauI GCSGC 2 cut(s) 170, 332
TfiI GAWTC 2 cut(s) 115, 120
Tru1I TTAA 7 cut(s) 77, 432, 564, 708, 831, 893, 1170
Tru9I TTAA 7 cut(s) 77, 432, 564, 708, 831, 893, 1170
TscAI CASTG 3 cut(s) 330, 427, 1081
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 388, 1288
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 3 cut(s) 224, 590, 774
TspRI CASTG 3 cut(s) 330, 427, 1081
VpaK11BI GGWCC 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 1205
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 3 cut(s) 920, 971, 1365
Zsp2I ATGCAT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.