Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0016665

Physical Location & Seq

Genomic Coordinates Forward (+)

20028052 .. 20030055

Gene Structure 2004 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G54090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1416 bp

ATGGCTTCACTTCTTATTTTCCCCCACCTTCACCATTTTGATTCTTCACTGGACCGTAGAGAGGTACTAGTAGTTCGCCATTCACAAGCATCAAGGCGATTTCTCACTCCGAAAGCTTCAATTAATGGCAGCGGAATCACAAACGGAGCTGCAGCGGAAACCACCTCTAAACCTAGTCGCAAAGGGAGGAAGAAGAAGCAAACATCGACGGTAATTGAGAAAGATAACACCGAGACTGATCCTGAATTGAATCCGGAGTTAGCTGATTACGACGACGGGATCGAGTTTCCGTACGACGATCCGCCTCTTGTGTGTTGCTTCGGAGCTGTACAGAAGGAGTTTGTTCCGGTGGTGCGAGTCCATGATAATCCGATGCATCCTGATATGTACTCGCAGTGGAAGATGCTACAGTGGGATCCACCGGAGTTTGGGAGAGCTCCGGGAGGTCCGCCGTCGAATGTGGCGATCTCTCATGTACGGTTAGGTGGGAGAGCTGCGTTTATGGGGAAAGTAGGTGAAGATGATTTTGGAGATGAGCTTGTTCTGATGATGAATCAGGAGAGAGTTCAGACGAGAGCTGTGAAATTCGATGAGAATTCGAAAACTGCTTGTACTCGTGTCAAGATCAAGTTTAAGGACGGGAAAATGATGGCGGAGACTGTGAAAGAACCACCTGAGGACTCGCTATTTGCTTCAGAACTCAATTTAGCTGTGCTTAAAGAGGCGAGGATTTTTCATTTCAATTCAGAAGTGTTGACGTCTCCTACAATGCAATCAACTCTTTTTACGGCAATACAATGGTCTAAGAAGTTTGGTGGGCTTATTTTCTTTGACTTGAATCTCCCATTACCGCTGTGGAGATCGCGCAATGAGACTAGGAAGTTGATCAAGAAAGCATGGAATGAAGCAAACATCATTGAAGTTTCACAGCAAGAGCTTGAGTTTCTACTTGATGAAGATTACTACGAGAGGAGAAGAAACTACACACCACAGTACTTCGCTGAGGATTTTGATCAGACGAAAAACAGAAGAGACTATTACCATTACACACCAGAGGAAATCAAGTCATTGTGGCATGACAAGCTGAAGCTACTGGTTGTGACTGATGGAACACTTCGGCTTCATTACTATACTCCTACTTTTGATGGTGTTGTGATTGGAACAGAAGATGTGCTTATAACTCCTTTCACCTGTGATAGGACAGGTTCTGGTGATGCTGTTGTTGCAGGGATAATGAGAAAGCTAACAACTTGTCCTGAGATGTTTGAGGATCAGGATGTGATGGAGCGACAGCTCAGGTTTGCAGTTGCTGCTGGGATCATAGCGCAGTGGACAATTGGTGCAGTTAGAGGTTTCCCTACTGAAAGCGCGACACAGAATTTGAAAGAACAAGTTTATGTTCCATCAATGTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009295 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0018130 GO:0019438 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0042644 GO:0042646 GO:0042793 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043621 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PfkB SOUL

Protein Analysis

471

Amino Acids

53.78

Weight (kDa)

5.77

Isoelectric Point (pI)

50.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	134 - 443	2.9e-27	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016665)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54090
fragaria_vesca	FvH4_6g12270
malus_domestica	MD12G1158400.v1.1
prunus_persica	Prupe.6G268800_v2.0.a1
pyrus_communis	pycom12g15040
rosa_chinensis	RchiOBHm_Chr3g0464071
rosa_laevigata	RLG00000024724
rosa_multiflora	Rmu_sc0006492.1_g000020
rosa_roxburghii	Rroxscaffold_6G00416020
rosa_rugosa	Rorug03G0070600
rosa_samantha	Rh3BG131700 Rh3CG134000 Rh3DG133000
rosa_wichuraiana	Rw3G010770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1178
AatII	GACGTC	1 cut(s)	761
AccII	CGCG	2 cut(s)	865, 1370
AccIII	TCCGGA	1 cut(s)	253
AciI	CCGC	6 cut(s)	132, 155, 302, 449, 653, 851
AclWI	GGATC	7 cut(s)	233, 287, 293, 410, 423, 1278, 1325
AcsI	RAATTY	3 cut(s)	584, 595, 1378
AcuI	CTGAAG	2 cut(s)	678, 1106
AcyI	GRCGYC	1 cut(s)	758
AfaI	GTAC	7 cut(s)	66, 293, 330, 389, 477, 613, 995
AfiI	CCNNNNNNNGG	3 cut(s)	61, 428, 1197
AgsI	TTSAA	6 cut(s)	120, 250, 742, 838, 920, 1384
AhlI	ACTAGT	1 cut(s)	67
AjuI	GAANNNNNNNTTGG	2 cut(s)	510, 542
Alw21I	GWGCWC	1 cut(s)	439
Alw26I	GTCTC	5 cut(s)	227, 650, 765, 866, 1026
AlwI	GGATC	7 cut(s)	233, 287, 293, 410, 423, 1278, 1325
AlwNI	CAGNNNCTG	2 cut(s)	1208, 1310
Aor13HI	TCCGGA	1 cut(s)	253
ApeKI	GCWGC	5 cut(s)	129, 149, 152, 494, 1310
ApoI	RAATTY	3 cut(s)	584, 595, 1378
ArsI	GACNNNNNNTTYG	4 cut(s)	671, 703, 1363, 1395
AseI	ATTAAT	1 cut(s)	123
AspLEI	GCGC	3 cut(s)	867, 1327, 1370
AspS9I	GGNCC	2 cut(s)	52, 446
AsuC2I	CCSGG	1 cut(s)	441
AsuHPI	GGTGA	4 cut(s)	23, 527, 1180, 1223
AsuII	TTCGAA	1 cut(s)	599
AvaII	GGWCC	2 cut(s)	52, 446
AxyI	CCTNAGG	1 cut(s)	675
BamHI	GGATCC	1 cut(s)	415
BanII	GRGCYC	1 cut(s)	439
BauI	CACGAG	1 cut(s)	615
Bbv12I	GWGCWC	1 cut(s)	439
BbvCI	CCTCAGC	1 cut(s)	1002
BbvI	GCAGC	5 cut(s)	136, 141, 164, 481, 1297
BccI	CCATC	5 cut(s)	643, 1100, 1139, 1276, 1411
BceAI	ACGGC	2 cut(s)	436, 804
BclI	TGATCA	2 cut(s)	885, 1012
BcnI	CCSGG	1 cut(s)	441
BcoDI	GTCTC	5 cut(s)	227, 650, 765, 866, 1026
BcuI	ACTAGT	1 cut(s)	67
BfaI	CTAG	3 cut(s)	68, 174, 876
BfmI	CTRYAG	2 cut(s)	150, 407
BisI	GCNGC	5 cut(s)	130, 150, 153, 495, 1311
BlsI	GCNGC	5 cut(s)	131, 151, 154, 496, 1312
BmcAI	AGTACT	1 cut(s)	995
Bme1390I	CCNGG	1 cut(s)	441
Bme18I	GGWCC	2 cut(s)	52, 446
BmgT120I	GGNCC	2 cut(s)	52, 446
BmiI	GGNNCC	1 cut(s)	417
BmrFI	CCNGG	1 cut(s)	441
BmsI	GCATC	5 cut(s)	98, 363, 385, 393, 1204
Bpu10I	CCTNAGC	2 cut(s)	1002, 1295
Bpu14I	TTCGAA	1 cut(s)	599
BpuEI	CTTGAG	1 cut(s)	959
BpuMI	CCSGG	1 cut(s)	441
BsaBI	GATNNNNATC	1 cut(s)	1011
BsaHI	GRCGYC	1 cut(s)	758
BsaWI	WCCGGW	3 cut(s)	253, 346, 421
Bsc4I	CCNNNNNNNGG	3 cut(s)	61, 428, 1197
Bse1I	ACTGG	2 cut(s)	54, 1098
Bse21I	CCTNAGG	1 cut(s)	675
Bse3DI	GCAATG	1 cut(s)	874
Bse8I	GATNNNNATC	1 cut(s)	1011
BseAI	TCCGGA	1 cut(s)	253
BseGI	GGATG	2 cut(s)	376, 1282
BseJI	GATNNNNATC	1 cut(s)	1011
BseLI	CCNNNNNNNGG	3 cut(s)	61, 428, 1197
BseMI	GCAATG	1 cut(s)	874
BseMII	CTCAG	4 cut(s)	666, 993, 1248, 1309
BseNI	ACTGG	2 cut(s)	54, 1098
BseRI	GAGGAG	1 cut(s)	985
BseXI	GCAGC	5 cut(s)	136, 141, 164, 481, 1297
BseYI	CCCAGC	1 cut(s)	1313
BsgI	GTGCAG	1 cut(s)	1362
Bsh1236I	CGCG	2 cut(s)	865, 1370
BsiHKAI	GWGCWC	1 cut(s)	439
BsiSI	CCGG	4 cut(s)	254, 347, 422, 440
BsiWI	CGTACG	1 cut(s)	291
BslI	CCNNNNNNNGG	3 cut(s)	61, 428, 1197
BsmAI	GTCTC	5 cut(s)	227, 650, 765, 866, 1026
BsmBI	CGTCTC	1 cut(s)	765
Bsp119I	TTCGAA	1 cut(s)	599
Bsp1286I	GDGCHC	1 cut(s)	439
Bsp13I	TCCGGA	1 cut(s)	253
Bsp1407I	TGTACA	1 cut(s)	328
BspACI	CCGC	6 cut(s)	132, 155, 302, 449, 653, 851
BspCNI	CTCAG	4 cut(s)	667, 994, 1249, 1308
BspEI	TCCGGA	1 cut(s)	253
BspFNI	CGCG	2 cut(s)	865, 1370
BspLI	GGNNCC	1 cut(s)	417
BspMAI	CTGCAG	1 cut(s)	154
BspPI	GGATC	7 cut(s)	233, 287, 293, 410, 423, 1278, 1325
BspT104I	TTCGAA	1 cut(s)	599
BsrDI	GCAATG	1 cut(s)	874
BsrGI	TGTACA	1 cut(s)	328
BsrI	ACTGG	2 cut(s)	54, 1098
BssNI	GRCGYC	1 cut(s)	758
BssSI	CACGAG	1 cut(s)	615
Bst2BI	CACGAG	1 cut(s)	615
Bst4CI	ACNGT	6 cut(s)	56, 211, 411, 480, 661, 993
Bst6I	CTCTTC	1 cut(s)	1024
BstACI	GRCGYC	1 cut(s)	758
BstAPI	GCANNNNNTGC	1 cut(s)	1310
BstAUI	TGTACA	1 cut(s)	328
BstBI	TTCGAA	1 cut(s)	599
BstDEI	CTNAG	5 cut(s)	675, 804, 1002, 1257, 1295
BstENI	CCTNNNNNAGG	1 cut(s)	1195
BstF5I	GGATG	2 cut(s)	376, 1282
BstFNI	CGCG	2 cut(s)	865, 1370
BstHHI	GCGC	3 cut(s)	867, 1327, 1370
BstMAI	GTCTC	5 cut(s)	227, 650, 765, 866, 1026
BstMWI	GCNNNNNNNGC	3 cut(s)	1081, 1223, 1310
BstSCI	CCNGG	1 cut(s)	439
BstSFI	CTRYAG	2 cut(s)	150, 407
BstUI	CGCG	2 cut(s)	865, 1370
BstV1I	GCAGC	5 cut(s)	136, 141, 164, 481, 1297
BstX2I	RGATCY	1 cut(s)	415
BstXI	CCANNNNNNTGG	1 cut(s)	1410
BstYI	RGATCY	1 cut(s)	415
Bsu36I	CCTNAGG	1 cut(s)	675
BtsCI	GGATG	2 cut(s)	376, 1282
BtsI	GCAGTG	2 cut(s)	401, 1334
BtsIMutI	CAGTG	4 cut(s)	47, 401, 416, 1334
CaiI	CAGNNNCTG	2 cut(s)	1208, 1310
CfoI	GCGC	3 cut(s)	867, 1327, 1370
Cfr13I	GGNCC	2 cut(s)	52, 446
Csp6I	GTAC	7 cut(s)	65, 292, 329, 388, 476, 612, 994
CviAII	CATG	4 cut(s)	362, 473, 897, 1076
CviQI	GTAC	7 cut(s)	65, 292, 329, 388, 476, 612, 994
DdeI	CTNAG	5 cut(s)	675, 804, 1002, 1257, 1295
Eam1104I	CTCTTC	1 cut(s)	1024
EarI	CTCTTC	1 cut(s)	1024
EciI	GGCGGA	3 cut(s)	291, 438, 668
Ecl136II	GAGCTC	1 cut(s)	437
Eco24I	GRGCYC	1 cut(s)	439
Eco47I	GGWCC	2 cut(s)	52, 446
Eco53kI	GAGCTC	1 cut(s)	437
Eco57I	CTGAAG	2 cut(s)	678, 1106
Eco81I	CCTNAGG	1 cut(s)	675
EcoICRI	GAGCTC	1 cut(s)	437
EcoNI	CCTNNNNNAGG	1 cut(s)	1195
EcoRI	GAATTC	1 cut(s)	595
EcoT22I	ATGCAT	1 cut(s)	378
EcoT38I	GRGCYC	1 cut(s)	439
Esp3I	CGTCTC	1 cut(s)	765
FaeI	CATG	4 cut(s)	365, 476, 900, 1079
FalI	AAGNNNNNCTT	2 cut(s)	1158, 1190
FatI	CATG	4 cut(s)	361, 472, 896, 1075
FbaI	TGATCA	2 cut(s)	885, 1012
Fnu4HI	GCNGC	5 cut(s)	130, 150, 153, 495, 1311
FokI	GGATG	2 cut(s)	363, 1289
FriOI	GRGCYC	1 cut(s)	439
Fsp4HI	GCNGC	5 cut(s)	130, 150, 153, 495, 1311
FspBI	CTAG	3 cut(s)	68, 174, 876
GlaI	GCGC	3 cut(s)	866, 1326, 1369
GluI	GCNGC	5 cut(s)	130, 150, 153, 495, 1311
GsaI	CCCAGC	1 cut(s)	1317
HapII	CCGG	4 cut(s)	254, 347, 422, 440
HhaI	GCGC	3 cut(s)	867, 1327, 1370
Hin1I	GRCGYC	1 cut(s)	758
Hin1II	CATG	4 cut(s)	365, 476, 900, 1079
Hin6I	GCGC	3 cut(s)	865, 1325, 1368
HinP1I	GCGC	3 cut(s)	865, 1325, 1368
HincII	GTYRAC	1 cut(s)	756
HindII	GTYRAC	1 cut(s)	756
HindIII	AAGCTT	1 cut(s)	114
HinfI	GANTC	7 cut(s)	41, 135, 250, 357, 553, 680, 838
HpaII	CCGG	4 cut(s)	254, 347, 422, 440
HphI	GGTGA	4 cut(s)	23, 527, 1180, 1223
Hpy166II	GTNNAC	2 cut(s)	756, 1332
Hpy188I	TCNGA	8 cut(s)	111, 323, 372, 546, 570, 697, 748, 1017
Hpy188III	TCNNGA	8 cut(s)	242, 254, 380, 557, 622, 889, 1256, 1274
Hpy8I	GTNNAC	2 cut(s)	756, 1332
Hpy99I	CGWCG	5 cut(s)	211, 275, 278, 299, 457
HpyAV	CCTTC	2 cut(s)	38, 328
HpyCH4III	ACNGT	6 cut(s)	56, 211, 411, 480, 661, 993
HpyCH4IV	ACGT	1 cut(s)	758
HpyCH4V	TGCA	6 cut(s)	152, 376, 772, 1226, 1304, 1343
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1081, 1223, 1310
HpyF3I	CTNAG	5 cut(s)	675, 804, 1002, 1257, 1295
HpySE526I	ACGT	1 cut(s)	758
Hsp92I	GRCGYC	1 cut(s)	758
Hsp92II	CATG	4 cut(s)	365, 476, 900, 1079
HspAI	GCGC	3 cut(s)	865, 1325, 1368
Kpn2I	TCCGGA	1 cut(s)	253
Ksp22I	TGATCA	2 cut(s)	885, 1012
LmnI	GCTCC	4 cut(s)	146, 323, 442, 1285
Lsp1109I	GCAGC	5 cut(s)	136, 141, 164, 481, 1297
LweI	GCATC	5 cut(s)	98, 363, 385, 393, 1204
MaeI	CTAG	3 cut(s)	68, 174, 876
MaeII	ACGT	1 cut(s)	758
MaeIII	GTNAC	1 cut(s)	1099
MboII	GAAGA	9 cut(s)	36, 202, 205, 412, 530, 968, 987, 1041, 1178
MfeI	CAATTG	1 cut(s)	1335
MflI	RGATCY	1 cut(s)	415
MhlI	GDGCHC	1 cut(s)	439
MluCI	AATT	9 cut(s)	120, 213, 245, 584, 595, 703, 742, 1335, 1378
MlyI	GAGTC	2 cut(s)	366, 674
Mph1103I	ATGCAT	1 cut(s)	378
MroI	TCCGGA	1 cut(s)	253
MseI	TTAA	3 cut(s)	123, 633, 717
MslI	CAYNNNNRTG	1 cut(s)	1408
MspA1I	CMGCKG	3 cut(s)	132, 155, 853
MspI	CCGG	4 cut(s)	254, 347, 422, 440
MspR9I	CCNGG	1 cut(s)	441
MunI	CAATTG	1 cut(s)	1335
MvnI	CGCG	2 cut(s)	865, 1370
MwoI	GCNNNNNNNGC	3 cut(s)	1081, 1223, 1310
NciI	CCSGG	1 cut(s)	441
NlaIII	CATG	4 cut(s)	365, 476, 900, 1079
NlaIV	GGNNCC	1 cut(s)	417
NmuCI	GTSAC	1 cut(s)	1099
NsiI	ATGCAT	1 cut(s)	378
NspV	TTCGAA	1 cut(s)	599
PcsI	WCGNNNNNNNCGW	2 cut(s)	279, 461
PfeI	GAWTC	5 cut(s)	41, 135, 250, 553, 838
Pfl23II	CGTACG	1 cut(s)	291
PfoI	TCCNGGA	1 cut(s)	439
PkrI	GCNGC	5 cut(s)	131, 151, 154, 496, 1312
PleI	GAGTC	2 cut(s)	365, 674
PpsI	GAGTC	2 cut(s)	365, 674
PshBI	ATTAAT	1 cut(s)	123
PsiI	TTATAA	1 cut(s)	1178
Psp124BI	GAGCTC	1 cut(s)	439
PspFI	CCCAGC	1 cut(s)	1313
PspLI	CGTACG	1 cut(s)	291
PspN4I	GGNNCC	1 cut(s)	417
PspPI	GGNCC	2 cut(s)	52, 446
PsrI	GAACNNNNNNTAC	2 cut(s)	57, 89
PstI	CTGCAG	1 cut(s)	154
PstNI	CAGNNNCTG	2 cut(s)	1208, 1310
PsuI	RGATCY	1 cut(s)	415
RsaI	GTAC	7 cut(s)	66, 293, 330, 389, 477, 613, 995
RsaNI	GTAC	7 cut(s)	65, 292, 329, 388, 476, 612, 994
RseI	CAYNNNNRTG	1 cut(s)	1408
SacI	GAGCTC	1 cut(s)	439
SaqAI	TTAA	3 cut(s)	123, 633, 717
SatI	GCNGC	5 cut(s)	130, 150, 153, 495, 1311
Sau96I	GGNCC	2 cut(s)	52, 446
ScaI	AGTACT	1 cut(s)	995
SchI	GAGTC	2 cut(s)	366, 674
ScrFI	CCNGG	1 cut(s)	441
SduI	GDGCHC	1 cut(s)	439
SfaNI	GCATC	5 cut(s)	98, 363, 385, 393, 1204
SfcI	CTRYAG	2 cut(s)	150, 407
SfuI	TTCGAA	1 cut(s)	599
SinI	GGWCC	2 cut(s)	52, 446
SmiMI	CAYNNNNRTG	1 cut(s)	1408
SmlI	CTYRAG	1 cut(s)	938
SmoI	CTYRAG	1 cut(s)	938
SpeI	ACTAGT	1 cut(s)	67
Sse9I	AATT	9 cut(s)	120, 213, 245, 584, 595, 703, 742, 1335, 1378
SsiI	CCGC	6 cut(s)	132, 155, 302, 449, 653, 851
SspMI	CTAG	3 cut(s)	68, 174, 876
SstI	GAGCTC	1 cut(s)	439
StyD4I	CCNGG	1 cut(s)	439
TaaI	ACNGT	6 cut(s)	56, 211, 411, 480, 661, 993
TaiI	ACGT	1 cut(s)	761
TaqI	TCGA	5 cut(s)	206, 282, 455, 588, 599
TasI	AATT	9 cut(s)	120, 213, 245, 584, 595, 703, 742, 1335, 1378
TatI	WGTACW	4 cut(s)	328, 387, 611, 993
TfiI	GAWTC	5 cut(s)	41, 135, 250, 553, 838
Tru1I	TTAA	3 cut(s)	123, 633, 717
Tru9I	TTAA	3 cut(s)	123, 633, 717
TscAI	CASTG	4 cut(s)	54, 401, 416, 1334
TseFI	GTSAC	1 cut(s)	1099
TseI	GCWGC	5 cut(s)	129, 149, 152, 494, 1310
Tsp45I	GTSAC	1 cut(s)	1099
TspDTI	ATGAA	5 cut(s)	566, 725, 918, 969, 1112
TspGWI	ACGGA	2 cut(s)	159, 279
TspRI	CASTG	4 cut(s)	54, 401, 416, 1334
VpaK11BI	GGWCC	2 cut(s)	52, 446
VspI	ATTAAT	1 cut(s)	123
XagI	CCTNNNNNAGG	1 cut(s)	1195
XapI	RAATTY	3 cut(s)	584, 595, 1378
XcmI	CCANNNNNNNNNTGG	1 cut(s)	852
XspI	CTAG	3 cut(s)	68, 174, 876
ZraI	GACGTC	1 cut(s)	759
ZrmI	AGTACT	1 cut(s)	995
Zsp2I	ATGCAT	1 cut(s)	378

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.