Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0016665

Physical Location & Seq

Genomic Coordinates Forward (+)

23893508 .. 23896073

Gene Structure 2566 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1158400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGCTTCCTTTCACCTCTCTCTCCCGCTTGTCATAAGCCCCAGAAGCGTCATTTCGAATGGGGTAGCCTTAGAATCTTTAAAGTTAAAAGCTTTCAATCCACGGTTTTTGCTACTTAAACCCCCCAAATCGCACAATCTAAACCCGCCCAGAGCTTCCACCAATGGCGCCGACAACGGAAGCATTGAACCCAGCAAACCCACTCGCCGCGGCAGGAAAAAGGGGCCAACTTCTTCTTCTTCGGAAGCACCGAAAAAACCCAAGCGTTCGAAGAAATCCGAGACCGTGAACGGCGCCGTCGAACCCGAACCCGTAATCCAACCCGAAAATCCGGATTCGGAACTCGAGGATTACGACGACGGCATAGATTTCCCGTACGATGACCCGCCTCTGGTTTGCTGCTTCGGCGCCGCGCAGAAGGAGTTCGTACCGACGGTTCGGGTCTCCGACAACCGGATGCACCCGGATATATACTCGCAGTGGAAAATGCTTCAGTGGGACCCGCCGGAGTTTGTGAGAGCCCCCGGCGGGTCGCCCTCCAATGTAGCGATTTCGCACGTGCGGCTCGGTGGGAGGGCGGCGTTTATGGGGAAGGTCGGGGAGGACGATTTTGGGGATGAGCTGGTGCTGACGATGAACAAGGAGAGGGTTCAGACGCGCGCGGTGAAGTTCGATTCGAGTGCGAAGACAGCGATGACGTACATGAAGATTAAATTTGACGATGAGAGGATGTGGGCGGAGACGGTGAAGGAGTCCGCCGAGGACTCGCTGCTCTGCTCCGAGCTCAACCTCGCCGTGCTCAAAGAGGCCAGAATGTTCCACTTCACTTCGGAAGTTTTGACGTCTCCCTCAATGCGACCAACCCTTTTCAGAGCAATGTCATATTCCAAAAAGTTTGGGGGGCTTGTGTTTTTTGATTTGAACTTGCCGTTGCCTCTGTGGAAATCGCGTGATGAAACAAGGAAGTACATTGAGAGAGCCTGGAACCAAGCTGACATAATCGAGGTTTCGAGGCAGGAATTGGAATTTCTTCTTGATGAAGACTACTACGTGAGGAAGAGGAATTACAGGCCCCAGTACTATGCTGAGGAGTTTGAGCAAACCAAGAAGCGGAGAGATTATTACCATTACACGCCCGAAGAAATATCTCCATTATGGCACGATGGACTTAAGTTTCTCTTTGTGACGGATGGAACGCTTCGAATTCATTACTATTCCCCTTCGTTTCACGGTGTTGTGATTGGAACGGAAGATGTGCTCATAACCCCATTCACTTGTGATAGGACTGGTTCTGGTGACGCTGTTGTGGCTGCGATTATGAGAAAGCTGACCACTCACCCTGAGATGTATGAAGACCAAGATGTTTTGGAAAGGCAGCTTCGATTTGCAGTTGCTGCGGGAATAATATCGCAGTGGACGATCGGTGCTGTTAGAGGTTTTCCTACGGAAAGTGCAACTCAAAATCTGAAGGAGCAGGTTTATGTGCCTTCGATGTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009295 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0018130 GO:0019438 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0042644 GO:0042646 GO:0042793 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043621 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PfkB SOUL

Protein Analysis

500

Amino Acids

56.79

Weight (kDa)

6.27

Isoelectric Point (pI)

53.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	163 - 471	5e-30	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016665)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54090
fragaria_vesca	FvH4_6g12270
malus_domestica	MD12G1158400.v1.1
prunus_persica	Prupe.6G268800_v2.0.a1
pyrus_communis	pycom12g15040
rosa_chinensis	RchiOBHm_Chr3g0464071
rosa_laevigata	RLG00000024724
rosa_multiflora	Rmu_sc0006492.1_g000020
rosa_roxburghii	Rroxscaffold_6G00416020
rosa_rugosa	Rorug03G0070600
rosa_samantha	Rh3BG131700 Rh3CG134000 Rh3DG133000
rosa_wichuraiana	Rw3G010770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	845
Acc36I	ACCTGC	1 cut(s)	1465
AccB1I	GGYRCC	3 cut(s)	167, 293, 407
AccII	CGCG	6 cut(s)	210, 413, 658, 660, 662, 949
AccIII	TCCGGA	1 cut(s)	331
AcsI	RAATTY	3 cut(s)	713, 1025, 1203
AcuI	CTGAAG	2 cut(s)	476, 1487
AcvI	CACGTG	1 cut(s)	559
AcyI	GRCGYC	4 cut(s)	168, 294, 408, 842
AfaI	GTAC	5 cut(s)	377, 429, 701, 968, 1079
AfiI	CCNNNNNNNGG	4 cut(s)	391, 453, 528, 1110
AflII	CTTAAG	1 cut(s)	1169
AgsI	TTSAA	3 cut(s)	97, 188, 922
AjnI	CCWGG	1 cut(s)	980
AjuI	GAANNNNNNNTTGG	2 cut(s)	220, 252
AloI	GAACNNNNNNTCC	2 cut(s)	300, 332
AluBI	AGCT	7 cut(s)	92, 155, 622, 784, 992, 1327, 1378
AluI	AGCT	7 cut(s)	92, 155, 622, 784, 992, 1327, 1378
Alw21I	GWGCWC	3 cut(s)	786, 801, 1260
Alw26I	GTCTC	4 cut(s)	275, 448, 734, 849
AlwNI	CAGNNNCTG	1 cut(s)	1394
Ama87I	CYCGRG	1 cut(s)	344
Aor13HI	TCCGGA	1 cut(s)	331
AoxI	GGCC	3 cut(s)	224, 807, 1070
ApeKI	GCWGC	5 cut(s)	399, 769, 1310, 1375, 1394
ApoI	RAATTY	3 cut(s)	713, 1025, 1203
Asp700I	GAANNNNTTC	2 cut(s)	422, 1029
AspLEI	GCGC	6 cut(s)	170, 296, 410, 415, 660, 662
AspS9I	GGNCC	3 cut(s)	224, 499, 1071
AsuC2I	CCSGG	2 cut(s)	464, 525
AsuHPI	GGTGA	5 cut(s)	5, 676, 757, 1307, 1328
AsuII	TTCGAA	3 cut(s)	56, 269, 1201
AvaI	CYCGRG	1 cut(s)	344
AvaII	GGWCC	1 cut(s)	499
BanI	GGYRCC	3 cut(s)	167, 293, 407
BanII	GRGCYC	2 cut(s)	523, 786
BarI	GAAGNNNNNNTAC	2 cut(s)	1049, 1081
BbrPI	CACGTG	1 cut(s)	559
BbsI	GAAGAC	3 cut(s)	692, 1047, 1359
Bbv12I	GWGCWC	3 cut(s)	786, 801, 1260
BbvCI	CCTCAGC	1 cut(s)	1086
BbvI	GCAGC	5 cut(s)	386, 756, 1297, 1381, 1387
BccI	CCATC	2 cut(s)	1157, 1184
BceAI	ACGGC	5 cut(s)	281, 307, 376, 779, 913
BcgI	CGANNNNNNTGC	2 cut(s)	662, 696
BciT130I	CCWGG	1 cut(s)	982
BcnI	CCSGG	2 cut(s)	464, 525
BcoDI	GTCTC	4 cut(s)	275, 448, 734, 849
BfoI	RGCGCY	3 cut(s)	171, 297, 411
BfrI	CTTAAG	1 cut(s)	1169
BfuAI	ACCTGC	1 cut(s)	1465
BmcAI	AGTACT	1 cut(s)	1079
Bme1390I	CCNGG	3 cut(s)	464, 525, 982
Bme18I	GGWCC	1 cut(s)	499
BmeT110I	CYCGRG	1 cut(s)	344
BmgT120I	GGNCC	3 cut(s)	224, 499, 1071
BmiI	GGNNCC	8 cut(s)	169, 225, 295, 409, 500, 501, 986, 1073
BmrFI	CCNGG	3 cut(s)	464, 525, 982
BmrI	ACTGGG	1 cut(s)	1069
BmsI	GCATC	1 cut(s)	447
BmuI	ACTGGG	1 cut(s)	1069
BpiI	GAAGAC	3 cut(s)	692, 1047, 1359
Bpu10I	CCTNAGC	1 cut(s)	1086
Bpu14I	TTCGAA	3 cut(s)	56, 269, 1201
BpuMI	CCSGG	2 cut(s)	464, 525
BsaAI	YACGTR	2 cut(s)	559, 1051
BsaHI	GRCGYC	4 cut(s)	168, 294, 408, 842
BsaI	GGTCTC	2 cut(s)	275, 448
BsaJI	CCNNGG	4 cut(s)	101, 208, 523, 759
BsaWI	WCCGGW	2 cut(s)	331, 453
Bsc4I	CCNNNNNNNGG	4 cut(s)	391, 453, 528, 1110
Bse1I	ACTGG	2 cut(s)	1075, 1291
Bse3DI	GCAATG	1 cut(s)	882
BseAI	TCCGGA	1 cut(s)	331
BseBI	CCWGG	1 cut(s)	982
BseDI	CCNNGG	4 cut(s)	101, 208, 523, 759
BseGI	GGATG	4 cut(s)	462, 622, 735, 1195
BseLI	CCNNNNNNNGG	4 cut(s)	391, 453, 528, 1110
BseMI	GCAATG	1 cut(s)	882
BseMII	CTCAG	2 cut(s)	1077, 1332
BseNI	ACTGG	2 cut(s)	1075, 1291
BsePI	GCGCGC	1 cut(s)	658
BseRI	GAGGAG	1 cut(s)	1103
BseXI	GCAGC	5 cut(s)	386, 756, 1297, 1381, 1387
BseYI	CCCAGC	1 cut(s)	191
Bsh1236I	CGCG	6 cut(s)	210, 413, 658, 660, 662, 949
Bsh1285I	CGRYCG	1 cut(s)	1422
BshFI	GGCC	3 cut(s)	226, 809, 1072
BshNI	GGYRCC	3 cut(s)	167, 293, 407
BsiEI	CGRYCG	1 cut(s)	1422
BsiHKAI	GWGCWC	3 cut(s)	786, 801, 1260
BsiHKCI	CYCGRG	1 cut(s)	344
BsiSI	CCGG	5 cut(s)	332, 454, 464, 506, 525
BsiWI	CGTACG	1 cut(s)	375
BslFI	GGGAC	1 cut(s)	512
BslI	CCNNNNNNNGG	4 cut(s)	391, 453, 528, 1110
BsmAI	GTCTC	4 cut(s)	275, 448, 734, 849
BsmBI	CGTCTC	2 cut(s)	734, 849
BsmFI	GGGAC	1 cut(s)	512
BsnI	GGCC	3 cut(s)	226, 809, 1072
Bso31I	GGTCTC	2 cut(s)	275, 448
BsoBI	CYCGRG	1 cut(s)	344
Bsp119I	TTCGAA	3 cut(s)	56, 269, 1201
Bsp1286I	GDGCHC	4 cut(s)	523, 786, 801, 1260
Bsp13I	TCCGGA	1 cut(s)	331
Bsp143I	GATC	1 cut(s)	1419
BspANI	GGCC	3 cut(s)	226, 809, 1072
BspCNI	CTCAG	2 cut(s)	1078, 1333
BspEI	TCCGGA	1 cut(s)	331
BspFNI	CGCG	6 cut(s)	210, 413, 658, 660, 662, 949
BspLI	GGNNCC	8 cut(s)	169, 225, 295, 409, 500, 501, 986, 1073
BspMI	ACCTGC	1 cut(s)	1465
BspT104I	TTCGAA	3 cut(s)	56, 269, 1201
BspT107I	GGYRCC	3 cut(s)	167, 293, 407
BspTI	CTTAAG	1 cut(s)	1169
BspTNI	GGTCTC	2 cut(s)	275, 448
BsrDI	GCAATG	1 cut(s)	882
BsrI	ACTGG	2 cut(s)	1075, 1291
BssECI	CCNNGG	4 cut(s)	101, 208, 523, 759
BssHII	GCGCGC	1 cut(s)	658
BssMI	GATC	1 cut(s)	1419
BssNI	GRCGYC	4 cut(s)	168, 294, 408, 842
Bst2UI	CCWGG	1 cut(s)	982
Bst4CI	ACNGT	5 cut(s)	105, 286, 436, 745, 1232
Bst6I	CTCTTC	1 cut(s)	1052
BstACI	GRCGYC	4 cut(s)	168, 294, 408, 842
BstAFI	CTTAAG	1 cut(s)	1169
BstAPI	GCANNNNNTGC	1 cut(s)	1394
BstBAI	YACGTR	2 cut(s)	559, 1051
BstBI	TTCGAA	3 cut(s)	56, 269, 1201
BstC8I	GCNNGC	1 cut(s)	660
BstDEI	CTNAG	3 cut(s)	70, 1086, 1341
BstDSI	CCRYGG	2 cut(s)	101, 208
BstF5I	GGATG	4 cut(s)	462, 622, 735, 1195
BstFNI	CGCG	6 cut(s)	210, 413, 658, 660, 662, 949
BstH2I	RGCGCY	3 cut(s)	171, 297, 411
BstHHI	GCGC	6 cut(s)	170, 296, 410, 415, 660, 662
BstKTI	GATC	1 cut(s)	1422
BstMAI	GTCTC	4 cut(s)	275, 448, 734, 849
BstMBI	GATC	1 cut(s)	1419
BstMCI	CGRYCG	1 cut(s)	1422
BstMWI	GCNNNNNNNGC	6 cut(s)	45, 405, 562, 689, 1307, 1394
BstNI	CCWGG	1 cut(s)	982
BstSCI	CCNGG	3 cut(s)	462, 523, 980
BstUI	CGCG	6 cut(s)	210, 413, 658, 660, 662, 949
BstV1I	GCAGC	5 cut(s)	386, 756, 1297, 1381, 1387
BstV2I	GAAGAC	3 cut(s)	692, 1047, 1359
BsuRI	GGCC	3 cut(s)	226, 809, 1072
BtgI	CCRYGG	2 cut(s)	101, 208
BtgZI	GCGATG	1 cut(s)	707
BtsCI	GGATG	4 cut(s)	462, 622, 735, 1195
BtsI	GCAGTG	2 cut(s)	485, 1418
BtsIMutI	CAGTG	3 cut(s)	485, 500, 1418
BveI	ACCTGC	1 cut(s)	1465
Cac8I	GCNNGC	1 cut(s)	660
CaiI	CAGNNNCTG	1 cut(s)	1394
CfoI	GCGC	6 cut(s)	170, 296, 410, 415, 660, 662
Cfr13I	GGNCC	3 cut(s)	224, 499, 1071
Cfr42I	CCGCGG	1 cut(s)	211
CseI	GACGC	3 cut(s)	37, 664, 1307
Csp6I	GTAC	5 cut(s)	376, 428, 700, 967, 1078
CspCI	CAANNNNNGTGG	2 cut(s)	90, 125
CviAII	CATG	1 cut(s)	703
CviQI	GTAC	5 cut(s)	376, 428, 700, 967, 1078
DdeI	CTNAG	3 cut(s)	70, 1086, 1341
DinI	GGCGCC	3 cut(s)	169, 295, 409
DpnI	GATC	1 cut(s)	1421
DpnII	GATC	1 cut(s)	1419
DraI	TTTAAA	1 cut(s)	81
Eam1104I	CTCTTC	1 cut(s)	1052
EarI	CTCTTC	1 cut(s)	1052
EciI	GGCGGA	2 cut(s)	745, 752
Ecl136II	GAGCTC	1 cut(s)	784
Eco24I	GRGCYC	2 cut(s)	523, 786
Eco31I	GGTCTC	2 cut(s)	275, 448
Eco47I	GGWCC	1 cut(s)	499
Eco53kI	GAGCTC	1 cut(s)	784
Eco57I	CTGAAG	2 cut(s)	476, 1487
Eco72I	CACGTG	1 cut(s)	559
Eco88I	CYCGRG	1 cut(s)	344
EcoICRI	GAGCTC	1 cut(s)	784
EcoO109I	RGGNCCY	2 cut(s)	499, 1071
EcoRI	GAATTC	1 cut(s)	1203
EcoRII	CCWGG	1 cut(s)	980
EcoT38I	GRGCYC	2 cut(s)	523, 786
EgeI	GGCGCC	3 cut(s)	169, 295, 409
EheI	GGCGCC	3 cut(s)	169, 295, 409
Esp3I	CGTCTC	2 cut(s)	734, 849
FaeI	CATG	1 cut(s)	706
FalI	AAGNNNNNCTT	2 cut(s)	1163, 1195
FaqI	GGGAC	1 cut(s)	512
FatI	CATG	1 cut(s)	702
FauI	CCCGC	6 cut(s)	33, 153, 393, 510, 521, 1390
FokI	GGATG	4 cut(s)	469, 629, 742, 1202
FriOI	GRGCYC	2 cut(s)	523, 786
GlaI	GCGC	6 cut(s)	169, 295, 409, 414, 659, 661
GsaI	CCCAGC	1 cut(s)	195
HaeII	RGCGCY	3 cut(s)	171, 297, 411
HaeIII	GGCC	3 cut(s)	226, 809, 1072
HapII	CCGG	5 cut(s)	332, 454, 464, 506, 525
HgaI	GACGC	3 cut(s)	37, 664, 1307
HhaI	GCGC	6 cut(s)	170, 296, 410, 415, 660, 662
Hin1I	GRCGYC	4 cut(s)	168, 294, 408, 842
Hin1II	CATG	1 cut(s)	706
Hin6I	GCGC	6 cut(s)	168, 294, 408, 413, 658, 660
HinP1I	GCGC	6 cut(s)	168, 294, 408, 413, 658, 660
HindIII	AAGCTT	1 cut(s)	90
HinfI	GANTC	5 cut(s)	74, 335, 674, 752, 764
HpaII	CCGG	5 cut(s)	332, 454, 464, 506, 525
HphI	GGTGA	5 cut(s)	5, 676, 757, 1307, 1328
Hpy166II	GTNNAC	2 cut(s)	289, 1416
Hpy188I	TCNGA	9 cut(s)	244, 280, 340, 448, 654, 781, 832, 872, 1467
Hpy188III	TCNNGA	2 cut(s)	332, 1034
Hpy8I	GTNNAC	2 cut(s)	289, 1416
Hpy99I	CGWCG	4 cut(s)	302, 359, 362, 436
HpyAV	CCTTC	6 cut(s)	412, 586, 742, 1230, 1462, 1497
HpyCH4III	ACNGT	5 cut(s)	105, 286, 436, 745, 1232
HpyCH4IV	ACGT	4 cut(s)	558, 698, 842, 1050
HpyCH4V	TGCA	3 cut(s)	460, 1388, 1454
HpyF10VI	GCNNNNNNNGC	6 cut(s)	45, 405, 562, 689, 1307, 1394
HpyF3I	CTNAG	3 cut(s)	70, 1086, 1341
HpySE526I	ACGT	4 cut(s)	558, 698, 842, 1050
Hsp92I	GRCGYC	4 cut(s)	168, 294, 408, 842
Hsp92II	CATG	1 cut(s)	706
HspAI	GCGC	6 cut(s)	168, 294, 408, 413, 658, 660
KasI	GGCGCC	3 cut(s)	167, 293, 407
KflI	GGGWCCC	1 cut(s)	499
Kpn2I	TCCGGA	1 cut(s)	331
KspI	CCGCGG	1 cut(s)	211
Kzo9I	GATC	1 cut(s)	1419
LmnI	GCTCC	2 cut(s)	782, 1471
Lsp1109I	GCAGC	5 cut(s)	386, 756, 1297, 1381, 1387
LweI	GCATC	1 cut(s)	447
MaeII	ACGT	4 cut(s)	558, 698, 842, 1050
MaeIII	GTNAC	2 cut(s)	1183, 1295
MalI	GATC	1 cut(s)	1421
MauBI	CGCGCGCG	1 cut(s)	658
MboI	GATC	1 cut(s)	1419
MhlI	GDGCHC	4 cut(s)	523, 786, 801, 1260
MluCI	AATT	5 cut(s)	713, 1019, 1025, 1063, 1203
Mly113I	GGCGCC	3 cut(s)	168, 294, 408
MlyI	GAGTC	2 cut(s)	758, 761
MmeI	TCCRAC	2 cut(s)	343, 471
MroI	TCCGGA	1 cut(s)	331
MroXI	GAANNNNTTC	2 cut(s)	422, 1029
MseI	TTAA	5 cut(s)	80, 86, 117, 711, 1170
MspA1I	CMGCKG	1 cut(s)	210
MspCI	CTTAAG	1 cut(s)	1169
MspI	CCGG	5 cut(s)	332, 454, 464, 506, 525
MspR9I	CCNGG	3 cut(s)	464, 525, 982
MteI	GCGCNGCGC	1 cut(s)	411
MvaI	CCWGG	1 cut(s)	982
MvnI	CGCG	6 cut(s)	210, 413, 658, 660, 662, 949
MwoI	GCNNNNNNNGC	6 cut(s)	45, 405, 562, 689, 1307, 1394
NarI	GGCGCC	3 cut(s)	168, 294, 408
NciI	CCSGG	2 cut(s)	464, 525
NdeII	GATC	1 cut(s)	1419
NlaIII	CATG	1 cut(s)	706
NlaIV	GGNNCC	8 cut(s)	169, 225, 295, 409, 500, 501, 986, 1073
NmeAIII	GCCGAG	1 cut(s)	784
NmuCI	GTSAC	2 cut(s)	1183, 1295
NspV	TTCGAA	3 cut(s)	56, 269, 1201
PaeR7I	CTCGAG	1 cut(s)	344
PauI	GCGCGC	1 cut(s)	658
PcsI	WCGNNNNNNNCGW	5 cut(s)	248, 297, 351, 603, 1415
PdmI	GAANNNNTTC	2 cut(s)	422, 1029
PfeI	GAWTC	3 cut(s)	74, 335, 674
Pfl23II	CGTACG	1 cut(s)	375
Ple19I	CGATCG	1 cut(s)	1422
PleI	GAGTC	2 cut(s)	758, 760
PluTI	GGCGCC	3 cut(s)	171, 297, 411
PmaCI	CACGTG	1 cut(s)	559
PmlI	CACGTG	1 cut(s)	559
PpsI	GAGTC	2 cut(s)	758, 760
Ppu21I	YACGTR	2 cut(s)	559, 1051
PpuMI	RGGWCCY	1 cut(s)	499
Psp124BI	GAGCTC	1 cut(s)	786
Psp5II	RGGWCCY	1 cut(s)	499
Psp6I	CCWGG	1 cut(s)	980
PspCI	CACGTG	1 cut(s)	559
PspFI	CCCAGC	1 cut(s)	191
PspGI	CCWGG	1 cut(s)	980
PspLI	CGTACG	1 cut(s)	375
PspN4I	GGNNCC	8 cut(s)	169, 225, 295, 409, 500, 501, 986, 1073
PspPI	GGNCC	3 cut(s)	224, 499, 1071
PspPPI	RGGWCCY	1 cut(s)	499
PspXI	VCTCGAGB	1 cut(s)	344
PsrI	GAACNNNNNNTAC	2 cut(s)	420, 452
PstNI	CAGNNNCTG	1 cut(s)	1394
PteI	GCGCGC	1 cut(s)	658
PvuI	CGATCG	1 cut(s)	1422
RsaI	GTAC	5 cut(s)	377, 429, 701, 968, 1079
RsaNI	GTAC	5 cut(s)	376, 428, 700, 967, 1078
SacI	GAGCTC	1 cut(s)	786
SacII	CCGCGG	1 cut(s)	211
SaqAI	TTAA	5 cut(s)	80, 86, 117, 711, 1170
Sau3AI	GATC	1 cut(s)	1419
Sau96I	GGNCC	3 cut(s)	224, 499, 1071
ScaI	AGTACT	1 cut(s)	1079
SchI	GAGTC	2 cut(s)	758, 761
ScrFI	CCNGG	3 cut(s)	464, 525, 982
SduI	GDGCHC	4 cut(s)	523, 786, 801, 1260
SfaNI	GCATC	1 cut(s)	447
SfoI	GGCGCC	3 cut(s)	169, 295, 409
Sfr274I	CTCGAG	1 cut(s)	344
Sfr303I	CCGCGG	1 cut(s)	211
SfuI	TTCGAA	3 cut(s)	56, 269, 1201
SgrBI	CCGCGG	1 cut(s)	211
SinI	GGWCC	1 cut(s)	499
SlaI	CTCGAG	1 cut(s)	344
SmlI	CTYRAG	2 cut(s)	344, 1169
SmoI	CTYRAG	2 cut(s)	344, 1169
Sse9I	AATT	5 cut(s)	713, 1019, 1025, 1063, 1203
SspDI	GGCGCC	3 cut(s)	167, 293, 407
SstI	GAGCTC	1 cut(s)	786
StyD4I	CCNGG	3 cut(s)	462, 523, 980
TaaI	ACNGT	5 cut(s)	105, 286, 436, 745, 1232
TaiI	ACGT	4 cut(s)	561, 701, 845, 1053
TasI	AATT	5 cut(s)	713, 1019, 1025, 1063, 1203
TatI	WGTACW	2 cut(s)	966, 1077
TauI	GCSGC	5 cut(s)	210, 213, 413, 565, 581
TfiI	GAWTC	3 cut(s)	74, 335, 674
Tru1I	TTAA	5 cut(s)	80, 86, 117, 711, 1170
Tru9I	TTAA	5 cut(s)	80, 86, 117, 711, 1170
TscAI	CASTG	3 cut(s)	485, 500, 1418
TseFI	GTSAC	2 cut(s)	1183, 1295
TseI	GCWGC	5 cut(s)	399, 769, 1310, 1375, 1394
Tsp45I	GTSAC	2 cut(s)	1183, 1295
TspDTI	ATGAA	6 cut(s)	650, 719, 969, 1053, 1196, 1365
TspGWI	ACGGA	4 cut(s)	192, 1202, 1262, 1460
TspRI	CASTG	3 cut(s)	485, 500, 1418
Vha464I	CTTAAG	1 cut(s)	1169
VpaK11BI	GGWCC	1 cut(s)	499
XapI	RAATTY	3 cut(s)	713, 1025, 1203
XhoI	CTCGAG	1 cut(s)	344
XmnI	GAANNNNTTC	2 cut(s)	422, 1029
ZraI	GACGTC	1 cut(s)	843
ZrmI	AGTACT	1 cut(s)	1079

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.