AT3G54850

Modified RING finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011054
Physical Location & Seq
Forward (+)
20321353 .. 20324020
2668 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54850.1

Sequence Viewer

Length: 1899 bp
ATGGGATTAACGAATTGTTGTTCCCACGAGGAGCTAATGAGTCGACTCGTTGACTCCGTTAAAGAAATATCAGGGTTTTCATCTTCAAGGGGTTTTATTGGGAAGATCCAAGGCGATCTTGTTCGTAGGATCACGCTTCTCAGCCCTTTCTTCGAGGAATTGATTGACGTCAATGTTGAATTGAAAAAGGATCAGATTACAGGGTTTGAGGCTATGAGAATCGCTCTTGATTCAAGTCTTGAGCTTTTTCGATCGGTTAATGGAGGAAGCAAGCTTTTTCAGCTTTTCGATAGAGATTCTCTTGTGGAGAAGTTCCGTGACATGACAGTGGAGATAGAAGCAGCGTTAAGTCAGATTCCTTATGAGAAGATTGAGGTATCAGAGGAAGTCAGAGAACAGGTTCAGCTTCTGCATTTTCAGTTCAAGAGAGCAAAAGAAAGATGGGAGGAGTCTGATCTACAGCTTAGCCATGATCTAGCTATGGCAGAGAATGTGATGGATCCTGACCCTATAATCCTCAAAAGACTTTCACAAGAGCTCCAACTTACTACCATTGATGAGCTGAAGAAAGAATCGCATGCGATACATGAGTATTTTCTTTCATATGATGGAGATCCTGATGACTGTTTCGAGAGGATGTCTTCACTTCTTAAAAACCTGGTAGACTTTGTAACAATGGAAAGTTCAGACCCTGATCCATCCACTGGCAGCAGAATCGTTTCGAGACATCGTTCTCCTGTTATACCAGAGTATTTTCGGTGTCCGATATCACTTGAACTGATGAAGGATCCTGTTATCGTCTCCACTGGACAGACATATGAAAGATCATCAATTCAGAAGTGGCTTGATGCTGGTCATAAAACATGTCCGAAATCTCAGGAGACACTTTTACATGCTGGATTAACCCCTAATTATGTGTTAAAGAGTCTCATTGCTTTGTGGTGTGAAAGCAACGGCATTGAGCTACCGCAAAATCAAGGGAGCTGTAGAACCACAAAAATAGGAGGAAGCAGCTCTTCAGATTGTGATCGAACATTTGTCCTTTCCTTGTTAGAGAAATTGGCCAACGGTACTACAGAACAGCAAAGAGCTGCAGCTGGAGAATTAAGGTTACTAGCCAAGAGGAACGTGGATAACAGAGTTTGTATCGCTGAGGCTGGAGCCATACCACTCCTTGTAGAGCTTCTATCCTCACCAGATCCTCGGACTCAGGAACATTCTGTGACAGCTCTTCTGAATCTTTCCATAAATGAAGGGAACAAAGGAGCCATTGTTGATGCAGGAGCCATAACGGATATAGTAGAAGTCCTAAAGAACGGAAGCATGGAAGCTAGAGAGAACGCTGCTGCAACCCTTTTCAGTTTATCTGTTATAGATGAAAACAAAGTGGCAATAGGTGCTGCTGGAGCTATCCAAGCACTTATAAGCTTGCTTGAGGAAGGAACCCGAAGAGGCAAAAAAGATGCTGCTACAGCGATTTTCAACTTATGCATATACCAGGGGAACAAATCAAGGGCGGTTAAAGGCGGTATTGTTGACCCTCTGACCAGATTACTGAAAGATGCAGGTGGCGGAATGGTGGATGAGGCTCTGGCCATTTTAGCAATTCTTTCAACTAACCAAGAAGGGAAAACAGCGATAGCTGAAGCAGAATCTATCCCGGTTTTGGTTGAGATTATAAGGACAGGGTCACCAAGGAACCGGGAAAATGCTGCAGCAATACTTTGGTATCTATGTATTGGGAATATAGAAAGGCTAAATGTAGCAAGAGAGGTTGGTGCAGATGTGGCCTTGAAGGAACTTACTGAGAATGGCACTGATAGAGCAAAGAGGAAAGCTGCGAGCTTGTTGGAGCTTATTCAGCAAACCGAAGGTGTTGCAGTAACTACTGTTCCATGA

Protein Analysis

632

Amino Acids

69.4

Weight (kDa)

5.07

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB10_N PF25368 38 - 145 3.8e-24 PUB10-like, N-terminal domain
U-box PF04564 248 - 318 2.2e-21 U-box domain
ARM_ARMC5 PF24768 347 - 521 9.1e-11 ARMC5
ARM_PUB PF25598 348 - 621 2.7e-26 Plant U-box protein ARM repeat region
Arm PF00514 376 - 415 1.4e-10 Armadillo/beta-catenin-like repeat
Arm PF00514 459 - 497 1e-05 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54850
fragaria_vesca FvH4_7g18840 FvH4_7g18840 FvH4_7g18840
malus_domestica MD01G1103500.v1.1 MD07G1170400.v1.1
prunus_persica Prupe.2G209300_v2.0.a1
pyrus_communis pycom01g13000 pycom07g16430
rosa_chinensis RchiOBHm_Chr1g0362651
rosa_laevigata RLG00000027631
rosa_multiflora Rmu_sc0000046.1_g000030
rosa_roxburghii Rroxscaffold_4G00293880
rosa_rugosa Rorug01G0300300.1 Rorug01G0300400
rosa_samantha Rh1AG308900 Rh1BG272700 Rh1CG290000 Rh1DG303200
rosa_wichuraiana Rw1G027460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1424, 1679
AarI CACCTGC 1 cut(s) 1556
AatII GACGTC 1 cut(s) 171
Acc36I ACCTGC 1 cut(s) 1556
AccB7I CCANNNNNTGG 1 cut(s) 704
AccI GTMKAC 2 cut(s) 43, 663
AciI CCGC 4 cut(s) 968, 1517, 1527, 1572
AcoI YGGCCR 2 cut(s) 1062, 1593
AcuI CTGAAG 3 cut(s) 584, 1002, 1665
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 1 cut(s) 1072
AfiI CCNNNNNNNGG 2 cut(s) 704, 1666
AflIII ACRYGT 1 cut(s) 863
AgsI TTSAA 9 cut(s) 87, 179, 184, 234, 424, 776, 1483, 1614, 1795
AjnI CCWGG 2 cut(s) 657, 1497
Alw21I GWGCWC 1 cut(s) 540
Alw26I GTCTC 4 cut(s) 718, 805, 875, 932
AlwNI CAGNNNCTG 2 cut(s) 409, 692
AoxI GGCC 3 cut(s) 1062, 1593, 1788
Asp700I GAANNNNTTC 2 cut(s) 399, 718
AsuC2I CCSGG 2 cut(s) 1661, 1703
AsuHPI GGTGA 2 cut(s) 1185, 1683
BaeI ACNNNNGTAYC 4 cut(s) 575, 608, 1712, 1745
BalI TGGCCA 2 cut(s) 1064, 1595
BamHI GGATCC 2 cut(s) 499, 787
BanII GRGCYC 1 cut(s) 540
BauI CACGAG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 540
BbvCI CCTCAGC 1 cut(s) 1152
BccI CCATC 4 cut(s) 435, 490, 602, 706
BceAI ACGGC 1 cut(s) 970
BcgI CGANNNNNNTGC 4 cut(s) 697, 731, 1859, 1893
BciT130I CCWGG 2 cut(s) 659, 1499
BcnI CCSGG 2 cut(s) 1661, 1703
BcoDI GTCTC 4 cut(s) 718, 805, 875, 932
BfaI CTAG 3 cut(s) 476, 1115, 1332
BfmI CTRYAG 6 cut(s) 458, 985, 1074, 1092, 1470, 1713
BfuAI ACCTGC 1 cut(s) 1556
BlpI GCTNAGC 1 cut(s) 464
Bme1390I CCNGG 4 cut(s) 659, 1499, 1661, 1703
BmiI GGNNCC 7 cut(s) 501, 789, 1162, 1267, 1285, 1444, 1700
BmrFI CCNGG 4 cut(s) 659, 1499, 1661, 1703
BmsI GCATC 4 cut(s) 838, 1267, 1453, 1552
BpiI GAAGAC 1 cut(s) 633
BplI GAGNNNNNCTC 2 cut(s) 208, 240
BpmI CTGGAG 3 cut(s) 1119, 1179, 1425
Bpu10I CCTNAGC 1 cut(s) 1152
Bpu1102I GCTNAGC 1 cut(s) 464
BpuEI CTTGAG 2 cut(s) 260, 1454
BpuMI CCSGG 2 cut(s) 1661, 1703
BsaBI GATNNNNATC 2 cut(s) 612, 1026
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 4 cut(s) 109, 1202, 1498, 1694
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 1257, 1287
Bsc4I CCNNNNNNNGG 2 cut(s) 704, 1666
Bse1I ACTGG 2 cut(s) 709, 811
Bse3DI GCAATG 1 cut(s) 930
Bse8I GATNNNNATC 2 cut(s) 612, 1026
BseBI CCWGG 2 cut(s) 659, 1499
BseDI CCNNGG 4 cut(s) 109, 1202, 1498, 1694
BseGI GGATG 3 cut(s) 642, 698, 1588
BseJI GATNNNNATC 2 cut(s) 612, 1026
BseLI CCNNNNNNNGG 2 cut(s) 704, 1666
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 5 cut(s) 154, 890, 1143, 1223, 1797
BseNI ACTGG 2 cut(s) 709, 811
BseRI GAGGAG 2 cut(s) 44, 461
BsgI GTGCAG 1 cut(s) 1800
Bsh1285I CGRYCG 1 cut(s) 254
BshFI GGCC 3 cut(s) 1064, 1595, 1790
BsiEI CGRYCG 1 cut(s) 254
BsiHKAI GWGCWC 1 cut(s) 540
BsiSI CCGG 2 cut(s) 1661, 1702
BslI CCNNNNNNNGG 2 cut(s) 704, 1666
BsmAI GTCTC 4 cut(s) 718, 805, 875, 932
BsmBI CGTCTC 1 cut(s) 805
BsnI GGCC 3 cut(s) 1064, 1595, 1790
Bsp1286I GDGCHC 1 cut(s) 540
Bsp1720I GCTNAGC 1 cut(s) 464
BspACI CCGC 4 cut(s) 968, 1517, 1527, 1572
BspANI GGCC 3 cut(s) 1064, 1595, 1790
BspCNI CTCAG 5 cut(s) 153, 889, 1144, 1222, 1798
BspLI GGNNCC 7 cut(s) 501, 789, 1162, 1267, 1285, 1444, 1700
BspMAI CTGCAG 2 cut(s) 1096, 1717
BspMI ACCTGC 1 cut(s) 1556
BspQI GCTCTTC 2 cut(s) 1021, 1236
BsrDI GCAATG 1 cut(s) 930
BsrI ACTGG 2 cut(s) 709, 811
BssECI CCNNGG 4 cut(s) 109, 1202, 1498, 1694
BssNI GRCGYC 1 cut(s) 168
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 2 cut(s) 109, 1694
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 2 cut(s) 659, 1499
Bst4CI ACNGT 4 cut(s) 328, 626, 1070, 1891
Bst6I CTCTTC 3 cut(s) 1021, 1236, 1444
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 1397
BstC8I GCNNGC 4 cut(s) 272, 579, 1430, 1843
BstDEI CTNAG 6 cut(s) 140, 464, 876, 1152, 1209, 1806
BstEII GGTNACC 1 cut(s) 1689
BstF5I GGATG 3 cut(s) 642, 698, 1588
BstMAI GTCTC 4 cut(s) 718, 805, 875, 932
BstMCI CGRYCG 1 cut(s) 254
BstMWI GCNNNNNNNGC 8 cut(s) 280, 1397, 1406, 1415, 1472, 1601, 1787, 1861
BstNI CCWGG 2 cut(s) 659, 1499
BstNSI RCATGY 3 cut(s) 581, 867, 896
BstPI GGTNACC 1 cut(s) 1689
BstSCI CCNGG 4 cut(s) 657, 1497, 1659, 1701
BstSFI CTRYAG 6 cut(s) 458, 985, 1074, 1092, 1470, 1713
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 5 cut(s) 105, 499, 613, 787, 1198
BstYI RGATCY 5 cut(s) 105, 499, 613, 787, 1198
BsuRI GGCC 3 cut(s) 1064, 1595, 1790
BtsCI GGATG 3 cut(s) 642, 698, 1588
BtsIMutI CAGTG 4 cut(s) 333, 702, 804, 1815
BveI ACCTGC 1 cut(s) 1556
Cac8I GCNNGC 4 cut(s) 272, 579, 1430, 1843
CaiI CAGNNNCTG 2 cut(s) 409, 692
CsiI ACCWGGT 1 cut(s) 657
Csp6I GTAC 1 cut(s) 1071
CviAII CATG 8 cut(s) 322, 470, 578, 587, 864, 893, 1324, 1896
CviQI GTAC 1 cut(s) 1071
DdeI CTNAG 6 cut(s) 140, 464, 876, 1152, 1209, 1806
EaeI YGGCCR 2 cut(s) 1062, 1593
Eam1104I CTCTTC 3 cut(s) 1021, 1236, 1444
EarI CTCTTC 3 cut(s) 1021, 1236, 1444
EciI GGCGGA 1 cut(s) 1587
Ecl136II GAGCTC 1 cut(s) 538
Eco130I CCWWGG 2 cut(s) 109, 1694
Eco24I GRGCYC 1 cut(s) 540
Eco32I GATATC 1 cut(s) 768
Eco53kI GAGCTC 1 cut(s) 538
Eco57I CTGAAG 3 cut(s) 584, 1002, 1665
Eco91I GGTNACC 1 cut(s) 1689
EcoICRI GAGCTC 1 cut(s) 538
EcoO65I GGTNACC 1 cut(s) 1689
EcoRII CCWGG 2 cut(s) 657, 1497
EcoRV GATATC 1 cut(s) 768
EcoT14I CCWWGG 2 cut(s) 109, 1694
EcoT22I ATGCAT 1 cut(s) 1493
EcoT38I GRGCYC 1 cut(s) 540
ErhI CCWWGG 2 cut(s) 109, 1694
Esp3I CGTCTC 1 cut(s) 805
FaeI CATG 8 cut(s) 325, 473, 581, 590, 867, 896, 1327, 1899
FalI AAGNNNNNCTT 4 cut(s) 102, 134, 1000, 1032
FatI CATG 8 cut(s) 321, 469, 577, 586, 863, 892, 1323, 1895
FauNDI CATATG 2 cut(s) 604, 817
FblI GTMKAC 2 cut(s) 43, 663
FokI GGATG 3 cut(s) 649, 685, 1595
FriOI GRGCYC 1 cut(s) 540
FspBI CTAG 3 cut(s) 476, 1115, 1332
GsuI CTGGAG 3 cut(s) 1119, 1179, 1425
HaeIII GGCC 3 cut(s) 1064, 1595, 1790
HapII CCGG 2 cut(s) 1661, 1702
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 8 cut(s) 325, 473, 581, 590, 867, 896, 1327, 1899
HincII GTYRAC 3 cut(s) 44, 52, 1537
HindII GTYRAC 3 cut(s) 44, 52, 1537
HindIII AAGCTT 2 cut(s) 272, 1426
HpaII CCGG 2 cut(s) 1661, 1702
HphI GGTGA 2 cut(s) 1185, 1683
Hpy166II GTNNAC 4 cut(s) 44, 52, 664, 1537
Hpy188III TCNNGA 9 cut(s) 227, 239, 424, 503, 617, 631, 723, 878, 1211
Hpy8I GTNNAC 4 cut(s) 44, 52, 664, 1537
HpyAV CCTTC 6 cut(s) 778, 1247, 1433, 1619, 1789, 1865
HpyCH4III ACNGT 4 cut(s) 328, 626, 1070, 1891
HpyCH4IV ACGT 2 cut(s) 168, 1128
HpyCH4V TGCA 9 cut(s) 412, 1094, 1280, 1349, 1491, 1565, 1715, 1781, 1880
HpyF10VI GCNNNNNNNGC 8 cut(s) 280, 1397, 1406, 1415, 1472, 1601, 1787, 1861
HpyF3I CTNAG 6 cut(s) 140, 464, 876, 1152, 1209, 1806
HpySE526I ACGT 2 cut(s) 168, 1128
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 8 cut(s) 325, 473, 581, 590, 867, 896, 1327, 1899
LguI GCTCTTC 2 cut(s) 1021, 1236
LmnI GCTCC 8 cut(s) 31, 543, 981, 1160, 1265, 1283, 1406, 1852
LweI GCATC 4 cut(s) 838, 1267, 1453, 1552
MabI ACCWGGT 1 cut(s) 657
MaeI CTAG 3 cut(s) 476, 1115, 1332
MaeII ACGT 2 cut(s) 168, 1128
MaeIII GTNAC 6 cut(s) 317, 670, 1110, 1222, 1689, 1882
MboII GAAGA 9 cut(s) 75, 115, 142, 379, 577, 633, 1008, 1223, 1461
MflI RGATCY 5 cut(s) 105, 499, 613, 787, 1198
MhlI GDGCHC 1 cut(s) 540
MlsI TGGCCA 2 cut(s) 1064, 1595
MluCI AATT 8 cut(s) 13, 158, 179, 831, 910, 1058, 1103, 1605
MluNI TGGCCA 2 cut(s) 1064, 1595
MlyI GAGTC 6 cut(s) 39, 47, 49, 458, 934, 1201
MmeI TCCRAC 2 cut(s) 565, 1830
Mox20I TGGCCA 2 cut(s) 1064, 1595
Mph1103I ATGCAT 1 cut(s) 1493
MroXI GAANNNNTTC 2 cut(s) 399, 718
MscI TGGCCA 2 cut(s) 1064, 1595
MseI TTAA 9 cut(s) 8, 60, 258, 347, 651, 902, 920, 1106, 1521
MslI CAYNNNNRTG 1 cut(s) 326
Msp20I TGGCCA 2 cut(s) 1064, 1595
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 2 cut(s) 1661, 1702
MspR9I CCNGG 4 cut(s) 659, 1499, 1661, 1703
MvaI CCWGG 2 cut(s) 659, 1499
MwoI GCNNNNNNNGC 8 cut(s) 280, 1397, 1406, 1415, 1472, 1601, 1787, 1861
NciI CCSGG 2 cut(s) 1661, 1703
NdeI CATATG 2 cut(s) 604, 817
NlaIII CATG 8 cut(s) 325, 473, 581, 590, 867, 896, 1327, 1899
NlaIV GGNNCC 7 cut(s) 501, 789, 1162, 1267, 1285, 1444, 1700
NmuCI GTSAC 3 cut(s) 317, 1222, 1689
NsiI ATGCAT 1 cut(s) 1493
NspI RCATGY 3 cut(s) 581, 867, 896
PaeI GCATGC 1 cut(s) 581
PaqCI CACCTGC 1 cut(s) 1556
PciI ACATGT 1 cut(s) 863
PciSI GCTCTTC 2 cut(s) 1021, 1236
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PdmI GAANNNNTTC 2 cut(s) 399, 718
PfeI GAWTC 8 cut(s) 219, 230, 296, 355, 572, 714, 1237, 1652
PflFI GACNNNGTC 1 cut(s) 1687
PflMI CCANNNNNTGG 1 cut(s) 704
Ple19I CGATCG 1 cut(s) 254
PleI GAGTC 6 cut(s) 39, 47, 48, 457, 933, 1201
PpsI GAGTC 6 cut(s) 39, 47, 48, 457, 933, 1201
PscI ACATGT 1 cut(s) 863
PsiI TTATAA 2 cut(s) 1424, 1679
Psp124BI GAGCTC 1 cut(s) 540
Psp6I CCWGG 2 cut(s) 657, 1497
PspEI GGTNACC 1 cut(s) 1689
PspGI CCWGG 2 cut(s) 657, 1497
PspN4I GGNNCC 7 cut(s) 501, 789, 1162, 1267, 1285, 1444, 1700
PsrI GAACNNNNNNTAC 1 cut(s) 1875
PstI CTGCAG 2 cut(s) 1096, 1717
PstNI CAGNNNCTG 2 cut(s) 409, 692
PsuI RGATCY 5 cut(s) 105, 499, 613, 787, 1198
PsyI GACNNNGTC 1 cut(s) 1687
PvuI CGATCG 1 cut(s) 254
PvuII CAGCTG 1 cut(s) 1097
RsaI GTAC 1 cut(s) 1072
RsaNI GTAC 1 cut(s) 1071
RseI CAYNNNNRTG 1 cut(s) 326
SacI GAGCTC 1 cut(s) 540
SalI GTCGAC 1 cut(s) 42
SapI GCTCTTC 2 cut(s) 1021, 1236
SaqAI TTAA 9 cut(s) 8, 60, 258, 347, 651, 902, 920, 1106, 1521
SchI GAGTC 6 cut(s) 39, 47, 49, 458, 934, 1201
ScrFI CCNGG 4 cut(s) 659, 1499, 1661, 1703
SduI GDGCHC 1 cut(s) 540
SexAI ACCWGGT 1 cut(s) 657
SfaNI GCATC 4 cut(s) 838, 1267, 1453, 1552
SfcI CTRYAG 6 cut(s) 458, 985, 1074, 1092, 1470, 1713
SmiMI CAYNNNNRTG 1 cut(s) 326
SmlI CTYRAG 2 cut(s) 239, 1433
SmoI CTYRAG 2 cut(s) 239, 1433
SphI GCATGC 1 cut(s) 581
Sse9I AATT 8 cut(s) 13, 158, 179, 831, 910, 1058, 1103, 1605
SsiI CCGC 4 cut(s) 968, 1517, 1527, 1572
SspMI CTAG 3 cut(s) 476, 1115, 1332
SstI GAGCTC 1 cut(s) 540
StyD4I CCNGG 4 cut(s) 657, 1497, 1659, 1701
StyI CCWWGG 2 cut(s) 109, 1694
TaaI ACNGT 4 cut(s) 328, 626, 1070, 1891
TaiI ACGT 2 cut(s) 171, 1131
TaqI TCGA 7 cut(s) 43, 153, 250, 288, 630, 722, 1030
TasI AATT 8 cut(s) 13, 158, 179, 831, 910, 1058, 1103, 1605
TfiI GAWTC 8 cut(s) 219, 230, 296, 355, 572, 714, 1237, 1652
Tru1I TTAA 9 cut(s) 8, 60, 258, 347, 651, 902, 920, 1106, 1521
Tru9I TTAA 9 cut(s) 8, 60, 258, 347, 651, 902, 920, 1106, 1521
TscAI CASTG 4 cut(s) 333, 709, 811, 1822
TseFI GTSAC 3 cut(s) 317, 1222, 1689
Tsp45I GTSAC 3 cut(s) 317, 1222, 1689
TspDTI ATGAA 6 cut(s) 69, 591, 797, 834, 1266, 1392
TspGWI ACGGA 4 cut(s) 46, 305, 1307, 1332
TspRI CASTG 4 cut(s) 333, 709, 811, 1822
Tth111I GACNNNGTC 1 cut(s) 1687
Van91I CCANNNNNTGG 1 cut(s) 704
XceI RCATGY 3 cut(s) 581, 867, 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XmiI GTMKAC 2 cut(s) 43, 663
XmnI GAANNNNTTC 2 cut(s) 399, 718
XspI CTAG 3 cut(s) 476, 1115, 1332
ZraI GACGTC 1 cut(s) 169
Zsp2I ATGCAT 1 cut(s) 1493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.