AT3G60680

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014567
Physical Location & Seq
Forward (+)
22429960 .. 22432106
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60680.1

Sequence Viewer

Length: 1500 bp
ATGCGGGAAGTGGAAACAACGGCAATCACAGCAGCTCCTCCACAATTCTCTCAAATGTTTCAGAAATTAGCCATGGCCGTCAAGACCAAGACCTACGAGTTCTTCACCGAAGACGATAATGAGAGAACCGATGCTGAGGGTTTCTCTCTTCTCGATTCCTCCGAGGATTTTATCACCGATCAGAAAGTCGTCGTTCTTAAACCCGATAAGCCTCTTCTTAGTGCTTCTTCTCCGGGATCTCCTATTGAATCTCCGGTGAACGATGTTCAGACGAAGAATTTGGGTGTGGTAAGTGTTGTTAAACCTAATCAAAAGAAGCTTTCTCAGGTTAGGAAATTGGATACACAGATGGGATTGAGTTTGATTTCTTCTGTTTTCGCTACTGCTTCGTCTTTTGAAGCTTCGTATCTTCAATTGCAAGCTGCTCACGCGCCGTTTGTTGAGGAAAATGTCAAGGCTGCTGATAGAGCTCTCGTATCTAACCTCCAGAAATTGTCTGATTTGAAGCAGTTTTATAGGAATTATCGCCAAAGTTTGGATTTTGAGTCTGATTTAGCTATTGGGTCTTGCTTAGAATCTCGTGTTCAGGAGAATCAGAGCAAGCTTAGAGCTTTGGAAACCGTCTCAAATCGTTTACAAGCTGAGATGGATGCAAAGGATTTGCAGGTTTGGAGTCTGAGGAACAAATTAGGTGAGATTCAGAAATCTACTTCCAAGTTGTCTAAGAGATTGTCTTCAAATTCATCGTTGGATGTTCTGTTATCGGTTAGGGTTTTTGAGTCTCTGTTGTATGATGCCTTTAAAGCTACTCAGAAATTCACTAAGATTCTGATTGAGTTGATGGAAAAAGCTGGGTGGGATTTGGATTTAGTGGCTAAGTCTGTTCATCCTGAGGTAGATTATGCTAAGGAGAGACATAACCGGTATGCATTGTTGTCGTATGTTTGCTTGGGGATGTTTCGTGGTTTTGATGGAGAAGGATTTGATCTAAATGAGAATGATTATGAAGAGTCTGAGAGAAGTTCTGTAGATTCTTCCTTGAGGGAATTGATGCAACATGTATCGAGTAATCCGATGGAGCTTCTTGATAGAGATAAAGATTGTGCATTCTCTAGATTTTGCGATAAGAAGTATCACGAGCTGATTCATCCGAACATGGCATCTTCGATATTCAGTAACATGGATGAAAACGAAGCTGTGTTGAGTTCTTGGAGATCTTTGAGCACATTCTATGAATCGTTTGTCACGATGGCTAGCTCGATTTGGACCCTTCACAAGTTAGCCTTGTCCTTTGATCCTGCAGTTGAGATTTTCCAAGTTGAAAGCGGAGTGGAATTTTCGATTGTTTTCATGGAGAATGTCTTAAAAAGAAAACAAGATAAGAAGTTTTCGATGAGTCCTACTCGAGCCAAAGTAGGATTCACAGTGGTTCCCGGGTTTAAAATCGGGTGCACCGTGATTCAGTGTCAAGTTTACCTTACCGGCGGCTCCAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

499

Amino Acids

56.27

Weight (kDa)

5.53

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 116 - 243 9.6e-37 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 437 - 492 4e-19 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60680
fragaria_vesca FvH4_7g11240
malus_domestica MD02G1208300.v1.1 MD07G1117100.v1.1
prunus_persica Prupe.2G140200_v2.0.a1
pyrus_communis pycom02g17060 pycom07g10760
rosa_chinensis RchiOBHm_Chr1g0349381
rosa_laevigata RLG00000028553
rosa_multiflora Rmu_sc0004208.1_g000009
rosa_roxburghii Rroxscaffold_4G00305590
rosa_rugosa Rorug01G0203100
rosa_samantha Rh1AG220500 Rh1BG186500 Rh1CG205600 Rh1DG216000
rosa_wichuraiana Rw1G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 655
AccB7I CCANNNNNTGG 1 cut(s) 535
AccII CGCG 1 cut(s) 431
AciI CCGC 3 cut(s) 4, 1326, 1485
AclWI GGATC 2 cut(s) 244, 1289
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 4 cut(s) 277, 739, 815, 1334
AfiI CCNNNNNNNGG 1 cut(s) 535
AflIII ACRYGT 1 cut(s) 1057
AgeI ACCGGT 1 cut(s) 921
AgsI TTSAA 6 cut(s) 248, 398, 413, 505, 738, 1322
AjuI GAANNNNNNNTTGG 2 cut(s) 1403, 1435
Alw21I GWGCWC 3 cut(s) 472, 1226, 1454
Alw26I GTCTC 3 cut(s) 628, 786, 907
Alw44I GTGCAC 1 cut(s) 1450
AlwI GGATC 2 cut(s) 244, 1289
Ama87I CYCGRG 2 cut(s) 1404, 1433
AoxI GGCC 1 cut(s) 75
ApaLI GTGCAC 1 cut(s) 1450
ApeKI GCWGC 3 cut(s) 32, 422, 458
ApoI RAATTY 4 cut(s) 277, 739, 815, 1334
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
AsiGI ACCGGT 1 cut(s) 921
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 1 cut(s) 1266
AsuC2I CCSGG 3 cut(s) 234, 1434, 1435
AsuHPI GGTGA 4 cut(s) 97, 166, 268, 704
AsuNHI GCTAGC 1 cut(s) 1253
AvaI CYCGRG 2 cut(s) 1404, 1433
AvaII GGWCC 1 cut(s) 1266
AxyI CCTNAGG 1 cut(s) 891
BaeGI GKGCMC 1 cut(s) 1454
BanII GRGCYC 1 cut(s) 472
BauI CACGAG 2 cut(s) 579, 1136
BbsI GAAGAC 2 cut(s) 117, 726
Bbv12I GWGCWC 3 cut(s) 472, 1226, 1454
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 3 cut(s) 44, 409, 445
BccI CCATC 6 cut(s) 343, 640, 835, 965, 1069, 1243
BceAI ACGGC 3 cut(s) 36, 62, 418
BcgI CGANNNNNNTGC 2 cut(s) 918, 952
BciVI GTATCC 1 cut(s) 334
BcnI CCSGG 3 cut(s) 234, 1434, 1435
BcoDI GTCTC 3 cut(s) 628, 786, 907
BfaI CTAG 2 cut(s) 1113, 1254
BfmI CTRYAG 2 cut(s) 1026, 1299
BfuAI ACCTGC 1 cut(s) 655
BfuI GTATCC 1 cut(s) 334
BglII AGATCT 1 cut(s) 1214
BisI GCNGC 4 cut(s) 33, 423, 459, 1486
BlsI GCNGC 4 cut(s) 34, 424, 460, 1487
Bme1390I CCNGG 3 cut(s) 234, 1434, 1435
Bme18I GGWCC 1 cut(s) 1266
BmeT110I CYCGRG 2 cut(s) 1404, 1433
BmgT120I GGNCC 1 cut(s) 1266
BmiI GGNNCC 3 cut(s) 1268, 1431, 1489
BmrFI CCNGG 3 cut(s) 234, 1434, 1435
BmsI GCATC 5 cut(s) 121, 640, 784, 1041, 1169
BmtI GCTAGC 1 cut(s) 1257
BpiI GAAGAC 2 cut(s) 117, 726
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 1387, 1419
BpmI CTGGAG 1 cut(s) 470
Bpu10I CCTNAGC 2 cut(s) 135, 906
BpuEI CTTGAG 1 cut(s) 1060
BpuMI CCSGG 3 cut(s) 234, 1434, 1435
BsaJI CCNNGG 3 cut(s) 72, 162, 1433
BsaWI WCCGGW 2 cut(s) 253, 921
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse118I RCCGGY 2 cut(s) 921, 1481
Bse21I CCTNAGG 1 cut(s) 891
BseDI CCNNGG 3 cut(s) 72, 162, 1433
BseGI GGATG 6 cut(s) 655, 757, 886, 960, 1147, 1189
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMII CTCAG 7 cut(s) 126, 338, 633, 668, 824, 882, 1005
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 1454
BseXI GCAGC 3 cut(s) 44, 409, 445
BseYI CCCAGC 1 cut(s) 851
Bsh1236I CGCG 1 cut(s) 431
BshFI GGCC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 921
BsiHKAI GWGCWC 3 cut(s) 472, 1226, 1454
BsiHKCI CYCGRG 2 cut(s) 1404, 1433
BsiSI CCGG 5 cut(s) 233, 254, 922, 1434, 1482
BslI CCNNNNNNNGG 1 cut(s) 535
BsmAI GTCTC 3 cut(s) 628, 786, 907
BsmBI CGTCTC 1 cut(s) 628
BsmI GAATGC 1 cut(s) 1106
BsnI GGCC 1 cut(s) 77
BsoBI CYCGRG 2 cut(s) 1404, 1433
Bsp1286I GDGCHC 3 cut(s) 472, 1226, 1454
Bsp143I GATC 5 cut(s) 178, 236, 985, 1214, 1294
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 3 cut(s) 4, 1326, 1485
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 7 cut(s) 127, 337, 634, 669, 823, 883, 1006
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 3 cut(s) 1268, 1431, 1489
BspMAI CTGCAG 1 cut(s) 1303
BspMI ACCTGC 1 cut(s) 655
BspOI GCTAGC 1 cut(s) 1257
BspPI GGATC 2 cut(s) 244, 1289
BsrFI RCCGGY 2 cut(s) 921, 1481
BssAI RCCGGY 2 cut(s) 921, 1481
BssECI CCNNGG 3 cut(s) 72, 162, 1433
BssMI GATC 5 cut(s) 178, 236, 985, 1214, 1294
BssSI CACGAG 2 cut(s) 579, 1136
BssT1I CCWWGG 1 cut(s) 72
Bst2BI CACGAG 2 cut(s) 579, 1136
Bst4CI ACNGT 3 cut(s) 622, 1426, 1456
Bst6I CTCTTC 3 cut(s) 153, 219, 1002
BstC8I GCNNGC 3 cut(s) 420, 602, 1255
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 6 cut(s) 655, 757, 886, 960, 1147, 1189
BstFNI CGCG 1 cut(s) 431
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 5 cut(s) 181, 239, 988, 1217, 1297
BstMAI GTCTC 3 cut(s) 628, 786, 907
BstMBI GATC 5 cut(s) 178, 236, 985, 1214, 1294
BstMWI GCNNNNNNNGC 4 cut(s) 29, 428, 467, 803
BstNSI RCATGY 1 cut(s) 1061
BstSCI CCNGG 3 cut(s) 232, 1432, 1433
BstSFI CTRYAG 2 cut(s) 1026, 1299
BstSLI GKGCMC 1 cut(s) 1454
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 3 cut(s) 44, 409, 445
BstV2I GAAGAC 2 cut(s) 117, 726
BstX2I RGATCY 2 cut(s) 236, 1214
BstYI RGATCY 2 cut(s) 236, 1214
Bsu36I CCTNAGG 1 cut(s) 891
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 1 cut(s) 77
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 6 cut(s) 655, 757, 886, 960, 1147, 1189
BtsIMutI CAGTG 2 cut(s) 1431, 1469
BveI ACCTGC 1 cut(s) 655
Cac8I GCNNGC 3 cut(s) 420, 602, 1255
CfoI GCGC 1 cut(s) 433
Cfr10I RCCGGY 2 cut(s) 921, 1481
Cfr13I GGNCC 1 cut(s) 1266
Cfr9I CCCGGG 1 cut(s) 1433
CspAI ACCGGT 1 cut(s) 921
CviAII CATG 5 cut(s) 73, 1058, 1156, 1180, 1351
DpnI GATC 5 cut(s) 180, 238, 987, 1216, 1296
DpnII GATC 5 cut(s) 178, 236, 985, 1214, 1294
DraI TTTAAA 2 cut(s) 802, 1441
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 3 cut(s) 153, 219, 1002
EarI CTCTTC 3 cut(s) 153, 219, 1002
Ecl136II GAGCTC 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 1266
Eco53kI GAGCTC 1 cut(s) 470
Eco81I CCTNAGG 1 cut(s) 891
Eco88I CYCGRG 2 cut(s) 1404, 1433
EcoICRI GAGCTC 1 cut(s) 470
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 472
ErhI CCWWGG 1 cut(s) 72
Esp3I CGTCTC 1 cut(s) 628
FaeI CATG 5 cut(s) 76, 1061, 1159, 1183, 1354
FalI AAGNNNNNCTT 4 cut(s) 1268, 1300, 1461, 1493
FatI CATG 5 cut(s) 72, 1057, 1155, 1179, 1350
Fnu4HI GCNGC 4 cut(s) 33, 423, 459, 1486
FokI GGATG 6 cut(s) 662, 764, 873, 967, 1134, 1196
FriOI GRGCYC 1 cut(s) 472
Fsp4HI GCNGC 4 cut(s) 33, 423, 459, 1486
FspBI CTAG 2 cut(s) 1113, 1254
GlaI GCGC 1 cut(s) 432
GluI GCNGC 4 cut(s) 33, 423, 459, 1486
GsaI CCCAGC 1 cut(s) 855
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 1 cut(s) 77
HapII CCGG 5 cut(s) 233, 254, 922, 1434, 1482
HhaI GCGC 1 cut(s) 433
Hin1II CATG 5 cut(s) 76, 1061, 1159, 1183, 1354
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HindIII AAGCTT 3 cut(s) 317, 399, 602
HpaII CCGG 5 cut(s) 233, 254, 922, 1434, 1482
HphI GGTGA 4 cut(s) 97, 166, 268, 704
Hpy166II GTNNAC 4 cut(s) 259, 635, 1452, 1474
Hpy188III TCNNGA 9 cut(s) 82, 152, 487, 587, 890, 1085, 1113, 1136, 1246
Hpy8I GTNNAC 4 cut(s) 259, 635, 1452, 1474
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 2 cut(s) 971, 1280
HpyCH4III ACNGT 3 cut(s) 622, 1426, 1456
HpyCH4V TGCA 8 cut(s) 418, 653, 664, 929, 1054, 1106, 1301, 1452
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 428, 467, 803
Hsp92II CATG 5 cut(s) 76, 1061, 1159, 1183, 1354
HspAI GCGC 1 cut(s) 431
Kzo9I GATC 5 cut(s) 178, 236, 985, 1214, 1294
LmnI GCTCC 3 cut(s) 40, 1078, 1493
Lsp1109I GCAGC 3 cut(s) 44, 409, 445
LweI GCATC 5 cut(s) 121, 640, 784, 1041, 1169
MaeI CTAG 2 cut(s) 1113, 1254
MaeIII GTNAC 2 cut(s) 1175, 1243
MalI GATC 5 cut(s) 180, 238, 987, 1216, 1296
MboI GATC 5 cut(s) 178, 236, 985, 1214, 1294
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 2 cut(s) 236, 1214
MhlI GDGCHC 3 cut(s) 472, 1226, 1454
MlyI GAGTC 5 cut(s) 554, 682, 788, 1019, 1405
MmeI TCCRAC 1 cut(s) 729
Mph1103I ATGCAT 1 cut(s) 931
MseI TTAA 5 cut(s) 198, 300, 801, 1364, 1440
MspI CCGG 5 cut(s) 233, 254, 922, 1434, 1482
MspR9I CCNGG 3 cut(s) 234, 1434, 1435
MunI CAATTG 1 cut(s) 413
Mva1269I GAATGC 1 cut(s) 1106
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 4 cut(s) 29, 428, 467, 803
NciI CCSGG 3 cut(s) 234, 1434, 1435
NcoI CCATGG 1 cut(s) 72
NdeII GATC 5 cut(s) 178, 236, 985, 1214, 1294
NheI GCTAGC 1 cut(s) 1253
NlaIII CATG 5 cut(s) 76, 1061, 1159, 1183, 1354
NlaIV GGNNCC 3 cut(s) 1268, 1431, 1489
NmuCI GTSAC 1 cut(s) 1243
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 1 cut(s) 1061
PaeR7I CTCGAG 1 cut(s) 1404
PciI ACATGT 1 cut(s) 1057
PcsI WCGNNNNNNNCGW 4 cut(s) 159, 1070, 1244, 1452
PctI GAATGC 1 cut(s) 1106
PflMI CCANNNNNTGG 1 cut(s) 535
PfoI TCCNGGA 1 cut(s) 232
PinAI ACCGGT 1 cut(s) 921
PkrI GCNGC 4 cut(s) 34, 424, 460, 1487
PleI GAGTC 5 cut(s) 553, 681, 787, 1018, 1404
PpsI GAGTC 5 cut(s) 553, 681, 787, 1018, 1404
PscI ACATGT 1 cut(s) 1057
Psp124BI GAGCTC 1 cut(s) 472
PspFI CCCAGC 1 cut(s) 851
PspN4I GGNNCC 3 cut(s) 1268, 1431, 1489
PspPI GGNCC 1 cut(s) 1266
PspXI VCTCGAGB 1 cut(s) 1404
PstI CTGCAG 1 cut(s) 1303
PsuI RGATCY 2 cut(s) 236, 1214
SacI GAGCTC 1 cut(s) 472
SaqAI TTAA 5 cut(s) 198, 300, 801, 1364, 1440
SatI GCNGC 4 cut(s) 33, 423, 459, 1486
Sau3AI GATC 5 cut(s) 178, 236, 985, 1214, 1294
Sau96I GGNCC 1 cut(s) 1266
SchI GAGTC 5 cut(s) 554, 682, 788, 1019, 1405
ScrFI CCNGG 3 cut(s) 234, 1434, 1435
SduI GDGCHC 3 cut(s) 472, 1226, 1454
SfaNI GCATC 5 cut(s) 121, 640, 784, 1041, 1169
SfcI CTRYAG 2 cut(s) 1026, 1299
Sfr274I CTCGAG 1 cut(s) 1404
SinI GGWCC 1 cut(s) 1266
SlaI CTCGAG 1 cut(s) 1404
SmaI CCCGGG 1 cut(s) 1435
SmlI CTYRAG 2 cut(s) 1039, 1404
SmoI CTYRAG 2 cut(s) 1039, 1404
SsiI CCGC 3 cut(s) 4, 1326, 1485
SspMI CTAG 2 cut(s) 1113, 1254
SstI GAGCTC 1 cut(s) 472
StyD4I CCNGG 3 cut(s) 232, 1432, 1433
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 3 cut(s) 622, 1426, 1456
TaqI TCGA 7 cut(s) 153, 1064, 1166, 1259, 1340, 1391, 1405
TauI GCSGC 1 cut(s) 1488
Tru1I TTAA 5 cut(s) 198, 300, 801, 1364, 1440
Tru9I TTAA 5 cut(s) 198, 300, 801, 1364, 1440
TscAI CASTG 2 cut(s) 1431, 1469
TseFI GTSAC 1 cut(s) 1243
TseI GCWGC 3 cut(s) 32, 422, 458
Tsp45I GTSAC 1 cut(s) 1243
TspDTI ATGAA 7 cut(s) 732, 875, 1020, 1136, 1200, 1248, 1339
TspMI CCCGGG 1 cut(s) 1433
TspRI CASTG 2 cut(s) 1431, 1469
Van91I CCANNNNNTGG 1 cut(s) 535
VneI GTGCAC 1 cut(s) 1450
VpaK11BI GGWCC 1 cut(s) 1266
XapI RAATTY 4 cut(s) 277, 739, 815, 1334
XbaI TCTAGA 1 cut(s) 1112
XceI RCATGY 1 cut(s) 1061
XhoI CTCGAG 1 cut(s) 1404
XmaI CCCGGG 1 cut(s) 1433
XspI CTAG 2 cut(s) 1113, 1254
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.