RLG00000028553

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014567
Physical Location & Seq
Reverse (-)
24261941 .. 24263425
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028553

Sequence Viewer

Length: 1485 bp
ATGGATGGCTCTTCTAAACCTCCCCAAATCTCTGAGATGTTCTCCAAATTCGCTCTCGCTTTCAAAACCAAAACCTTTGAGTTCTTTGCTGAAGAGGAAGAAGACCCTGACAGTTTATCTCTGCTCGACTCCGCCGAAGAAATCATCACCGACCAGAAGGTCGTCGTCATCAAACCCGACGGAGCACCCAGTCCGACCTCGCCGGAGCTCCAAATAATACCGACGAAGCCCAATTTGGGCGAGACCCAGGTCCAAAGCCCAGAGCTTTCGACGATTACAAGGTCTCTGAGCAAAACCCAGATCAGACCCATCAATATTCCGATGACCCAGACTCTGATTTCGTCGGTCTTCGCCACCGTTTCGTCTTTCGAAGCTTCCTACCTCCAGTTACAGACGGCCCATGTGCCCTTTGTGGAAGAAAACGTGACGTCAGCGGACCGAGCTCTGGTCTCTCACCTTCAGAGGCTCTCGGAATTCAAGCAGTTTTTCAAGGATTTTTGTGGAAGTTCGGATATCGGGTCGGGTTTTGGAGTCGGGTCTTGCTTGGAGGCCCAGGTGCAAGAGAATCAGAGCAAGCTCAGGACTTTGGGGACCATGTCAAATAGGTTGCAGACAGAGATTGATCACAAAGACAATGAAGTTGTGGCCCTGAGGAAAAAACTGGGTGAGATTCAGAAATCTAACTTGAGACTATCTCAGAGGTTATGTGGAACTTTGAATTCTTCTTCTTGTGAGGTTTTGTTGTCAGTTAGAGTATTTGATTCGGTTCTACATGATGCTTGTAGATTAACACACAGATTTAGTAAGATTCTGATAAGTTTGATGGGAAAAGCGGGGTGGGATTTGGATTTGGCTGCTAATTTGGTTCATCCTGATGTTGGTTATGTGAAGAAGGCTCATAATCGATATGCGTTTTTGTCATATGTTTGTTTGGGAATGTTCAAAGGCTTTGATTCGAAAGATTTTGGTTTAGTTGAGGATGGGGTTTTGTGTAACGGGCATGGTTCGGAATTGGATAAGAACAATGTTCCTCTGAAGCAGTTGCTTGAGCATGTTTCGAGCAATCCGATGGAGTTGTTGAGTAGGAACCAGAATTGTGAGTTTTCGAGATTTTGTGAGAGCAAGTATCAAGAGATCATTCACCCCGCTATGGAATCTTCAATGTTCAGTAATTTTGATCGGAATGCAGTTGTGTTGAGTAAATGGAGGTCGTTGAGCGTATTCTATGAATCATTTGTTAACATGGCTAGCTCGATATGGACGTTACACAAGTTGGCCTATTGCTTCGATCCTGTGGTGGAGATATTCCAAGTGGAAAGAGATGCAGATTTTTCTATGGTATATATGGAAGATGTCACTAGGAGATTGTCATTGCCTAGTAAAACCAGGGAAAAAGTTGGCTTCACAGTGGTTCCGGGTTTTAAACTTGGAAGGACGATAATTCAGTCTCAGGTTTACCTAAGTGGCTTGAAATGTACAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

495

Amino Acids

55.38

Weight (kDa)

5.85

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 108 - 234 8e-38 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 435 - 486 2e-18 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60680
fragaria_vesca FvH4_7g11240
malus_domestica MD02G1208300.v1.1 MD07G1117100.v1.1
prunus_persica Prupe.2G140200_v2.0.a1
pyrus_communis pycom02g17060 pycom07g10760
rosa_chinensis RchiOBHm_Chr1g0349381
rosa_laevigata RLG00000028553
rosa_multiflora Rmu_sc0004208.1_g000009
rosa_roxburghii Rroxscaffold_4G00305590
rosa_rugosa Rorug01G0203100
rosa_samantha Rh1AG220500 Rh1BG186500 Rh1CG205600 Rh1DG216000
rosa_wichuraiana Rw1G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AatII GACGTC 1 cut(s) 431
AciI CCGC 4 cut(s) 132, 434, 833, 1146
AclWI GGATC 1 cut(s) 1283
AcsI RAATTY 3 cut(s) 47, 473, 718
AcuI CTGAAG 3 cut(s) 111, 443, 1055
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 1 cut(s) 1477
AfiI CCNNNNNNNGG 4 cut(s) 236, 445, 878, 1150
AgsI TTSAA 7 cut(s) 64, 478, 490, 718, 943, 1161, 1471
AjnI CCWGG 3 cut(s) 246, 552, 1385
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 6 cut(s) 208, 265, 374, 443, 577, 1251
AluI AGCT 6 cut(s) 208, 265, 374, 443, 577, 1251
Alw21I GWGCWC 3 cut(s) 187, 210, 445
Alw26I GTCTC 5 cut(s) 236, 288, 454, 682, 1452
AlwI GGATC 1 cut(s) 1283
AlwNI CAGNNNCTG 1 cut(s) 334
AoxI GGCC 4 cut(s) 396, 549, 645, 1275
ApeKI GCWGC 1 cut(s) 854
ApoI RAATTY 3 cut(s) 47, 473, 718
ArsI GACNNNNNNTTYG 2 cut(s) 322, 354
AspS9I GGNCC 6 cut(s) 250, 397, 436, 550, 591, 646
AsuC2I CCSGG 1 cut(s) 1416
AsuHPI GGTGA 4 cut(s) 139, 446, 677, 1133
AsuII TTCGAA 2 cut(s) 369, 956
AsuNHI GCTAGC 1 cut(s) 1247
AvaII GGWCC 3 cut(s) 250, 436, 591
AxyI CCTNAGG 1 cut(s) 650
BaeGI GKGCMC 1 cut(s) 408
BanII GRGCYC 2 cut(s) 210, 445
BbsI GAAGAC 2 cut(s) 108, 340
Bbv12I GWGCWC 3 cut(s) 187, 210, 445
BbvI GCAGC 1 cut(s) 841
BccI CCATC 4 cut(s) 317, 817, 974, 1063
BceAI ACGGC 1 cut(s) 411
BciT130I CCWGG 3 cut(s) 248, 554, 1387
BclI TGATCA 1 cut(s) 622
BcnI CCSGG 1 cut(s) 1416
BcoDI GTCTC 5 cut(s) 236, 288, 454, 682, 1452
BfaI CTAG 3 cut(s) 1248, 1359, 1377
BisI GCNGC 1 cut(s) 855
BlsI GCNGC 1 cut(s) 856
Bme1390I CCNGG 4 cut(s) 248, 554, 1387, 1416
Bme18I GGWCC 3 cut(s) 250, 436, 591
BmgT120I GGNCC 6 cut(s) 250, 397, 436, 550, 591, 646
BmiI GGNNCC 3 cut(s) 592, 1088, 1413
BmrFI CCNGG 4 cut(s) 248, 554, 1387, 1416
BmrI ACTGGG 2 cut(s) 183, 671
BmsI GCATC 2 cut(s) 766, 1312
BmtI GCTAGC 1 cut(s) 1251
BmuI ACTGGG 2 cut(s) 183, 671
BoxI GACNNNNGTC 1 cut(s) 248
BpiI GAAGAC 2 cut(s) 108, 340
BplI GAGNNNNNCTC 4 cut(s) 26, 58, 679, 711
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 578
Bpu14I TTCGAA 2 cut(s) 369, 956
BpuEI CTTGAG 2 cut(s) 706, 1067
BpuMI CCSGG 1 cut(s) 1416
Bsa29I ATCGAT 1 cut(s) 904
BsaHI GRCGYC 1 cut(s) 428
BsaI GGTCTC 3 cut(s) 236, 288, 454
BsaJI CCNNGG 3 cut(s) 246, 552, 1386
Bsc4I CCNNNNNNNGG 4 cut(s) 236, 445, 878, 1150
Bse1I ACTGG 3 cut(s) 189, 385, 666
Bse21I CCTNAGG 1 cut(s) 650
Bse3DI GCAATG 1 cut(s) 1370
BseBI CCWGG 3 cut(s) 248, 554, 1387
BseCI ATCGAT 1 cut(s) 904
BseDI CCNNGG 3 cut(s) 246, 552, 1386
BseGI GGATG 3 cut(s) 10, 868, 985
BseLI CCNNNNNNNGG 4 cut(s) 236, 445, 878, 1150
BseMI GCAATG 1 cut(s) 1370
BseMII CTCAG 6 cut(s) 24, 278, 592, 641, 710, 1463
BseNI ACTGG 3 cut(s) 189, 385, 666
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 1 cut(s) 841
BshFI GGCC 4 cut(s) 398, 551, 647, 1277
BshVI ATCGAT 1 cut(s) 904
BsiHKAI GWGCWC 3 cut(s) 187, 210, 445
BsiSI CCGG 2 cut(s) 203, 1415
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 4 cut(s) 236, 445, 878, 1150
BsmAI GTCTC 5 cut(s) 236, 288, 454, 682, 1452
BsmFI GGGAC 1 cut(s) 604
BsmI GAATGC 1 cut(s) 1189
BsnI GGCC 4 cut(s) 398, 551, 647, 1277
Bso31I GGTCTC 3 cut(s) 236, 288, 454
Bsp119I TTCGAA 2 cut(s) 369, 956
Bsp1286I GDGCHC 4 cut(s) 187, 210, 408, 445
Bsp1407I TGTACA 1 cut(s) 1475
Bsp143I GATC 5 cut(s) 300, 622, 1134, 1177, 1288
BspACI CCGC 4 cut(s) 132, 434, 833, 1146
BspANI GGCC 4 cut(s) 398, 551, 647, 1277
BspCNI CTCAG 6 cut(s) 25, 279, 591, 642, 709, 1462
BspDI ATCGAT 1 cut(s) 904
BspLI GGNNCC 3 cut(s) 592, 1088, 1413
BspOI GCTAGC 1 cut(s) 1251
BspPI GGATC 1 cut(s) 1283
BspQI GCTCTTC 1 cut(s) 16
BspT104I TTCGAA 2 cut(s) 369, 956
BspTNI GGTCTC 3 cut(s) 236, 288, 454
BsrDI GCAATG 1 cut(s) 1370
BsrGI TGTACA 1 cut(s) 1475
BsrI ACTGG 3 cut(s) 189, 385, 666
BssECI CCNNGG 3 cut(s) 246, 552, 1386
BssMI GATC 5 cut(s) 300, 622, 1134, 1177, 1288
BssNI GRCGYC 1 cut(s) 428
Bst2UI CCWGG 3 cut(s) 248, 554, 1387
Bst4CI ACNGT 3 cut(s) 113, 358, 1408
Bst6I CTCTTC 2 cut(s) 16, 87
BstACI GRCGYC 1 cut(s) 428
BstAUI TGTACA 1 cut(s) 1475
BstBI TTCGAA 2 cut(s) 369, 956
BstC8I GCNNGC 2 cut(s) 575, 1249
BstDEI CTNAG 7 cut(s) 33, 287, 578, 650, 696, 1449, 1460
BstF5I GGATG 3 cut(s) 10, 868, 985
BstKTI GATC 5 cut(s) 303, 625, 1137, 1180, 1291
BstMAI GTCTC 5 cut(s) 236, 288, 454, 682, 1452
BstMBI GATC 5 cut(s) 300, 622, 1134, 1177, 1288
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstNI CCWGG 3 cut(s) 248, 554, 1387
BstNSI RCATGY 1 cut(s) 1055
BstPAI GACNNNNGTC 1 cut(s) 248
BstSCI CCNGG 4 cut(s) 246, 552, 1385, 1414
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 841
BstV2I GAAGAC 2 cut(s) 108, 340
Bsu15I ATCGAT 1 cut(s) 904
Bsu36I CCTNAGG 1 cut(s) 650
BsuRI GGCC 4 cut(s) 398, 551, 647, 1277
BsuTUI ATCGAT 1 cut(s) 904
BtsCI GGATG 3 cut(s) 10, 868, 985
BtsIMutI CAGTG 1 cut(s) 1413
Cac8I GCNNGC 2 cut(s) 575, 1249
CaiI CAGNNNCTG 1 cut(s) 334
Cfr13I GGNCC 6 cut(s) 250, 397, 436, 550, 591, 646
ClaI ATCGAT 1 cut(s) 904
CpoI CGGWCCG 1 cut(s) 436
Csp6I GTAC 1 cut(s) 1476
CspI CGGWCCG 1 cut(s) 436
CviAII CATG 6 cut(s) 401, 595, 773, 1001, 1052, 1243
CviQI GTAC 1 cut(s) 1476
DdeI CTNAG 7 cut(s) 33, 287, 578, 650, 696, 1449, 1460
DpnI GATC 5 cut(s) 302, 624, 1136, 1179, 1290
DpnII GATC 5 cut(s) 300, 622, 1134, 1177, 1288
DraI TTTAAA 1 cut(s) 1423
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
Eam1104I CTCTTC 2 cut(s) 16, 87
EarI CTCTTC 2 cut(s) 16, 87
EciI GGCGGA 1 cut(s) 121
Ecl136II GAGCTC 2 cut(s) 208, 443
Eco24I GRGCYC 2 cut(s) 210, 445
Eco31I GGTCTC 3 cut(s) 236, 288, 454
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 3 cut(s) 250, 436, 591
Eco53kI GAGCTC 2 cut(s) 208, 443
Eco57I CTGAAG 3 cut(s) 111, 443, 1055
Eco81I CCTNAGG 1 cut(s) 650
EcoICRI GAGCTC 2 cut(s) 208, 443
EcoRI GAATTC 2 cut(s) 473, 718
EcoRII CCWGG 3 cut(s) 246, 552, 1385
EcoRV GATATC 1 cut(s) 514
EcoT38I GRGCYC 2 cut(s) 210, 445
FaeI CATG 6 cut(s) 404, 598, 776, 1004, 1055, 1246
FaqI GGGAC 1 cut(s) 604
FatI CATG 6 cut(s) 400, 594, 772, 1000, 1051, 1242
FauI CCCGC 2 cut(s) 826, 1153
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 622
Fnu4HI GCNGC 1 cut(s) 855
FokI GGATG 3 cut(s) 17, 855, 992
FriOI GRGCYC 2 cut(s) 210, 445
Fsp4HI GCNGC 1 cut(s) 855
FspBI CTAG 3 cut(s) 1248, 1359, 1377
GluI GCNGC 1 cut(s) 855
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 4 cut(s) 398, 551, 647, 1277
HapII CCGG 2 cut(s) 203, 1415
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 6 cut(s) 404, 598, 776, 1004, 1055, 1246
HincII GTYRAC 1 cut(s) 1240
HindII GTYRAC 1 cut(s) 1240
HindIII AAGCTT 1 cut(s) 372
HpaI GTTAAC 1 cut(s) 1240
HpaII CCGG 2 cut(s) 203, 1415
HphI GGTGA 4 cut(s) 139, 446, 677, 1133
Hpy166II GTNNAC 2 cut(s) 1240, 1456
Hpy188III TCNNGA 4 cut(s) 580, 872, 1107, 1130
Hpy8I GTNNAC 2 cut(s) 1240, 1456
Hpy99I CGWCG 5 cut(s) 167, 182, 226, 274, 346
HpyAV CCTTC 4 cut(s) 151, 467, 886, 1425
HpyCH4III ACNGT 3 cut(s) 113, 358, 1408
HpyCH4IV ACGT 3 cut(s) 423, 428, 1262
HpyCH4V TGCA 4 cut(s) 559, 610, 1187, 1325
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 7 cut(s) 33, 287, 578, 650, 696, 1449, 1460
HpySE526I ACGT 3 cut(s) 423, 428, 1262
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 6 cut(s) 404, 598, 776, 1004, 1055, 1246
Ksp22I TGATCA 1 cut(s) 622
KspAI GTTAAC 1 cut(s) 1240
Kzo9I GATC 5 cut(s) 300, 622, 1134, 1177, 1288
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 3 cut(s) 182, 205, 213
Lsp1109I GCAGC 1 cut(s) 841
LweI GCATC 2 cut(s) 766, 1312
MaeI CTAG 3 cut(s) 1248, 1359, 1377
MaeII ACGT 3 cut(s) 423, 428, 1262
MaeIII GTNAC 5 cut(s) 387, 424, 992, 1263, 1354
MalI GATC 5 cut(s) 302, 624, 1136, 1179, 1290
MboI GATC 5 cut(s) 300, 622, 1134, 1177, 1288
MhlI GDGCHC 4 cut(s) 187, 210, 408, 445
MluCI AATT 9 cut(s) 47, 232, 473, 718, 859, 1010, 1093, 1171, 1440
MlyI GAGTC 3 cut(s) 122, 325, 540
MmeI TCCRAC 1 cut(s) 218
MseI TTAA 3 cut(s) 788, 1239, 1422
MslI CAYNNNNRTG 1 cut(s) 873
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 2 cut(s) 203, 1415
MspR9I CCNGG 4 cut(s) 248, 554, 1387, 1416
Mva1269I GAATGC 1 cut(s) 1189
MvaI CCWGG 3 cut(s) 248, 554, 1387
MwoI GCNNNNNNNGC 1 cut(s) 440
NciI CCSGG 1 cut(s) 1416
NdeI CATATG 1 cut(s) 922
NdeII GATC 5 cut(s) 300, 622, 1134, 1177, 1288
NheI GCTAGC 1 cut(s) 1247
NlaIII CATG 6 cut(s) 404, 598, 776, 1004, 1055, 1246
NlaIV GGNNCC 3 cut(s) 592, 1088, 1413
NmuCI GTSAC 2 cut(s) 424, 1354
NspI RCATGY 1 cut(s) 1055
NspV TTCGAA 2 cut(s) 369, 956
PciSI GCTCTTC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 1064, 1259
PctI GAATGC 1 cut(s) 1189
PfeI GAWTC 7 cut(s) 565, 670, 761, 808, 953, 1154, 1229
PflFI GACNNNGTC 1 cut(s) 595
PkrI GCNGC 1 cut(s) 856
PleI GAGTC 3 cut(s) 122, 325, 539
PpsI GAGTC 3 cut(s) 122, 325, 539
PshAI GACNNNNGTC 1 cut(s) 248
Psp124BI GAGCTC 2 cut(s) 210, 445
Psp6I CCWGG 3 cut(s) 246, 552, 1385
PspGI CCWGG 3 cut(s) 246, 552, 1385
PspN4I GGNNCC 3 cut(s) 592, 1088, 1413
PspPI GGNCC 6 cut(s) 250, 397, 436, 550, 591, 646
PstNI CAGNNNCTG 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 595
RsaI GTAC 1 cut(s) 1477
RsaNI GTAC 1 cut(s) 1476
RseI CAYNNNNRTG 1 cut(s) 873
Rsr2I CGGWCCG 1 cut(s) 436
RsrII CGGWCCG 1 cut(s) 436
SacI GAGCTC 2 cut(s) 210, 445
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 3 cut(s) 788, 1239, 1422
SatI GCNGC 1 cut(s) 855
Sau3AI GATC 5 cut(s) 300, 622, 1134, 1177, 1288
Sau96I GGNCC 6 cut(s) 250, 397, 436, 550, 591, 646
SchI GAGTC 3 cut(s) 122, 325, 540
ScrFI CCNGG 4 cut(s) 248, 554, 1387, 1416
SduI GDGCHC 4 cut(s) 187, 210, 408, 445
SfaNI GCATC 2 cut(s) 766, 1312
SfuI TTCGAA 2 cut(s) 369, 956
SinI GGWCC 3 cut(s) 250, 436, 591
SmiMI CAYNNNNRTG 1 cut(s) 873
SmlI CTYRAG 2 cut(s) 685, 1046
SmoI CTYRAG 2 cut(s) 685, 1046
Sse9I AATT 9 cut(s) 47, 232, 473, 718, 859, 1010, 1093, 1171, 1440
SsiI CCGC 4 cut(s) 132, 434, 833, 1146
SspI AATATT 1 cut(s) 316
SspMI CTAG 3 cut(s) 1248, 1359, 1377
SstI GAGCTC 2 cut(s) 210, 445
StyD4I CCNGG 4 cut(s) 246, 552, 1385, 1414
TaaI ACNGT 3 cut(s) 113, 358, 1408
TaiI ACGT 3 cut(s) 426, 431, 1265
TaqI TCGA 9 cut(s) 126, 269, 369, 904, 956, 1058, 1106, 1253, 1287
TaqII GACCGA 2 cut(s) 334, 453
TasI AATT 9 cut(s) 47, 232, 473, 718, 859, 1010, 1093, 1171, 1440
TatI WGTACW 1 cut(s) 1475
TfiI GAWTC 7 cut(s) 565, 670, 761, 808, 953, 1154, 1229
Tru1I TTAA 3 cut(s) 788, 1239, 1422
Tru9I TTAA 3 cut(s) 788, 1239, 1422
TscAI CASTG 1 cut(s) 1413
TseFI GTSAC 2 cut(s) 424, 1354
TseI GCWGC 1 cut(s) 854
Tsp45I GTSAC 2 cut(s) 424, 1354
TspDTI ATGAA 3 cut(s) 651, 857, 1242
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 1413
Tth111I GACNNNGTC 1 cut(s) 595
VpaK11BI GGWCC 3 cut(s) 250, 436, 591
XapI RAATTY 3 cut(s) 47, 473, 718
XceI RCATGY 1 cut(s) 1055
XspI CTAG 3 cut(s) 1248, 1359, 1377
ZraI GACGTC 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.