MD07G1117100.v1.1

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014567
Physical Location & Seq
Forward (+)
14275883 .. 14277382
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1117100.v1.1.491

Sequence Viewer

Length: 1500 bp
ATGCTAGAAATGGACGACGGCACTTCGAAGCCTCCACAAATCTCCGAAATGTTTCAGAAATTCGCTCTGGCTTTCAAGGCCAAAACTTTCGAGTTCTTCGCCGAGGAGGAAGCCGACGACGCCGACAGCTTCGCGCTACTGGATTCTGCCGAAGAGGTCATCACGGACCAGAAGGTCGTCGTCATCAAACCGGACACGGCGGCGGCGAACGGCAACGCTCCGGAGATAGAATTAAAGCAGGAGAAGCCGAATTCGAGCGAGAACCAGGTGAAGAAACCGGAGGTTTTGGCAGTTTCAAGGCCTTTGAGGCGGACTCGGATCCGACCCATGAATACCCAGATGACCCAGACTCTGATTTCATCGATTTTTGCCACGGTTTCGTCGTTTGAAGCTTCGTATCTGCAATTGCAGACTGCCCATGTGCCGTTTGTGGAAGAAAATGTGACGGCAGCTGATCGAGCTCTGGTCTCTCACCTCCAGAGGCTCTCAGAATTCAAGCAGTTTTACAGAGATTTTTGTACGAGTTCTGATTCGGGTTCTGATTTGCCAATTGGGTCCTGCTTGGAAGCTCAGGTACAGGATAATCAGAGCAAGCTCAGGACTTTGGGAACAATGTCGAACAGGTTGCAGACAGAGATAGATCAAAAGGACAATGAAGTTACGGCCCTGCGGAAAAAATTGGGTGAGATTCAAAAGTCTAATGTGAAATTGTCTAAGAGGTTATCTGCTACTTTGAATTCTTCTTGTGATGTTTTGTTGTCCGTTAGAGTTTTCGATTCGGTTTTGCATGATGCTTGTAGATTAACACACAGATTTACAAAGATTTTGATAACTTTGATGGAGAAAGCTGGGTGGGATTTAGATTTGGCTGCTAATTTGGTTCATCCGGATGTTGATTACGTTAAAAGAGCGCATAACCAGTATGCTTTCTTGTCGTATGTTTGTTTGGGAATGTTCAAAGGTTTCGATTTGAAAGGTTTTGGTTTAGATGAGGAAGAAATTTCGTGTAATGGGCATGGTACGGATGTGGATAAGAAGAATGGTTCCTTGAAGCAGTTGCTTGAGCATGCGTCTAGCAATCCAATGGAGTTGTTAAGTAGGAATCAAAATTGTGAATTTTCAAGATTTTGTGAGAGCAAGTATCAAGAGATTATCCACCCCACTATGGAATCTTCAATCTTTAGTAATTTGGATCGAAACCAAGTTGTGTTGAGTTCATGGAGGTCTTTGAGTGTATTCTATGAGTCATTCGTTAGCATGGCGAGCTCGATATGGATGCTCCAAAAGTTGGCGTATTCCTTTGATCCAGCAGTTGAGATATTCCAAGTGGAAAGAGGTGTTGATTTTTCGATGGTTTATATGGAAGATGTCACTAGGAGAATGTTATTGCCTTGTAAAGCCAGGATGAAAGTTGGTTTTACAGTGGTTCCGGGTTTTAAGATTGGAAGGACAGTAATTCAATCCCAGGTATATTTAAGTGGCTTGAAATGTACAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

500

Amino Acids

56.3

Weight (kDa)

5.66

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 111 - 240 1.2e-40 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 440 - 491 4.6e-19 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60680
fragaria_vesca FvH4_7g11240
malus_domestica MD02G1208300.v1.1 MD07G1117100.v1.1
prunus_persica Prupe.2G140200_v2.0.a1
pyrus_communis pycom02g17060 pycom07g10760
rosa_chinensis RchiOBHm_Chr1g0349381
rosa_laevigata RLG00000028553
rosa_multiflora Rmu_sc0004208.1_g000009
rosa_roxburghii Rroxscaffold_4G00305590
rosa_rugosa Rorug01G0203100
rosa_samantha Rh1AG220500 Rh1BG186500 Rh1CG205600 Rh1DG216000
rosa_wichuraiana Rw1G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AccB7I CCANNNNNTGG 1 cut(s) 1288
AccII CGCG 1 cut(s) 134
AccIII TCCGGA 2 cut(s) 220, 886
AciI CCGC 4 cut(s) 200, 203, 310, 670
AclWI GGATC 4 cut(s) 313, 326, 1200, 1298
AcsI RAATTY 6 cut(s) 59, 250, 491, 736, 999, 1115
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 4 cut(s) 520, 576, 1021, 1492
AfiI CCNNNNNNNGG 2 cut(s) 1165, 1288
AjnI CCWGG 3 cut(s) 264, 1400, 1464
AluBI AGCT 8 cut(s) 129, 392, 452, 461, 569, 595, 848, 1266
AluI AGCT 8 cut(s) 129, 392, 452, 461, 569, 595, 848, 1266
Alw21I GWGCWC 2 cut(s) 463, 1268
Alw26I GTCTC 1 cut(s) 472
AlwI GGATC 4 cut(s) 313, 326, 1200, 1298
AlwNI CAGNNNCTG 1 cut(s) 352
Aor13HI TCCGGA 2 cut(s) 220, 886
AoxI GGCC 3 cut(s) 78, 299, 663
ApeKI GCWGC 2 cut(s) 449, 869
ApoI RAATTY 6 cut(s) 59, 250, 491, 736, 999, 1115
Asp700I GAANNNNTTC 1 cut(s) 51
AspLEI GCGC 2 cut(s) 136, 913
AspS9I GGNCC 3 cut(s) 166, 555, 664
AsuC2I CCSGG 1 cut(s) 1431
AsuHPI GGTGA 3 cut(s) 280, 464, 695
AsuII TTCGAA 1 cut(s) 26
AvaII GGWCC 2 cut(s) 166, 555
BaeI ACNNNNGTAYC 2 cut(s) 1011, 1044
BamHI GGATCC 1 cut(s) 318
BanII GRGCYC 2 cut(s) 463, 1268
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
Bbv12I GWGCWC 2 cut(s) 463, 1268
BbvI GCAGC 2 cut(s) 461, 856
BccI CCATC 2 cut(s) 832, 1345
BceAI ACGGC 6 cut(s) 34, 213, 226, 409, 462, 678
BcgI CGANNNNNNTGC 4 cut(s) 607, 641, 1258, 1292
BciT130I CCWGG 3 cut(s) 266, 1402, 1466
BcnI CCSGG 1 cut(s) 1431
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 3 cut(s) 5, 1074, 1374
BglI GCCNNNNNGGC 1 cut(s) 307
BisI GCNGC 4 cut(s) 201, 204, 450, 870
BlsI GCNGC 4 cut(s) 202, 205, 451, 871
Bme1390I CCNGG 4 cut(s) 266, 1402, 1431, 1466
Bme18I GGWCC 2 cut(s) 166, 555
BmgT120I GGNCC 3 cut(s) 166, 555, 664
BmiI GGNNCC 4 cut(s) 320, 556, 1045, 1428
BmrFI CCNGG 4 cut(s) 266, 1402, 1431, 1466
BmsI GCATC 2 cut(s) 781, 1266
BplI GAGNNNNNCTC 2 cut(s) 298, 330
BpmI CTGGAG 1 cut(s) 461
Bpu10I CCTNAGC 2 cut(s) 570, 596
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 1082
BpuMI CCSGG 1 cut(s) 1431
Bsa29I ATCGAT 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 472
BsaJI CCNNGG 3 cut(s) 102, 372, 1464
BsaWI WCCGGW 4 cut(s) 190, 220, 277, 886
Bsc4I CCNNNNNNNGG 2 cut(s) 1165, 1288
Bse1I ACTGG 2 cut(s) 144, 919
BseAI TCCGGA 2 cut(s) 220, 886
BseBI CCWGG 3 cut(s) 266, 1402, 1466
BseCI ATCGAT 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 102, 372, 1464
BseGI GGATG 5 cut(s) 883, 895, 1030, 1281, 1410
BseLI CCNNNNNNNGG 2 cut(s) 1165, 1288
BseMII CTCAG 3 cut(s) 501, 584, 610
BseNI ACTGG 2 cut(s) 144, 919
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 2 cut(s) 461, 856
BseYI CCCAGC 1 cut(s) 848
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 3 cut(s) 80, 301, 665
BshVI ATCGAT 1 cut(s) 362
BsiHKAI GWGCWC 2 cut(s) 463, 1268
BsiSI CCGG 5 cut(s) 191, 221, 278, 887, 1430
BslI CCNNNNNNNGG 2 cut(s) 1165, 1288
BsmAI GTCTC 1 cut(s) 472
BsnI GGCC 3 cut(s) 80, 301, 665
Bso31I GGTCTC 1 cut(s) 472
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 463, 1268
Bsp13I TCCGGA 2 cut(s) 220, 886
Bsp1407I TGTACA 1 cut(s) 1490
Bsp143I GATC 5 cut(s) 318, 454, 640, 1192, 1303
BspACI CCGC 4 cut(s) 200, 203, 310, 670
BspANI GGCC 3 cut(s) 80, 301, 665
BspCNI CTCAG 3 cut(s) 500, 583, 609
BspDI ATCGAT 1 cut(s) 362
BspEI TCCGGA 2 cut(s) 220, 886
BspFNI CGCG 1 cut(s) 134
BspLI GGNNCC 4 cut(s) 320, 556, 1045, 1428
BspPI GGATC 4 cut(s) 313, 326, 1200, 1298
BspT104I TTCGAA 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 472
BsrGI TGTACA 1 cut(s) 1490
BsrI ACTGG 2 cut(s) 144, 919
BssECI CCNNGG 3 cut(s) 102, 372, 1464
BssMI GATC 5 cut(s) 318, 454, 640, 1192, 1303
BssNI GRCGYC 1 cut(s) 120
Bst2UI CCWGG 3 cut(s) 266, 1402, 1466
Bst4CI ACNGT 3 cut(s) 376, 1423, 1453
Bst6I CTCTTC 1 cut(s) 147
BstACI GRCGYC 1 cut(s) 120
BstAUI TGTACA 1 cut(s) 1490
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 593, 1068, 1264
BstDEI CTNAG 4 cut(s) 487, 570, 596, 714
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 5 cut(s) 883, 895, 1030, 1281, 1410
BstFNI CGCG 1 cut(s) 134
BstHHI GCGC 2 cut(s) 136, 913
BstKTI GATC 5 cut(s) 321, 457, 643, 1195, 1306
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 5 cut(s) 318, 454, 640, 1192, 1303
BstMWI GCNNNNNNNGC 6 cut(s) 77, 119, 244, 307, 458, 1263
BstNI CCWGG 3 cut(s) 266, 1402, 1466
BstNSI RCATGY 1 cut(s) 1070
BstSCI CCNGG 4 cut(s) 264, 1400, 1429, 1464
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 2 cut(s) 461, 856
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
Bsu15I ATCGAT 1 cut(s) 362
BsuRI GGCC 3 cut(s) 80, 301, 665
BsuTUI ATCGAT 1 cut(s) 362
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 5 cut(s) 883, 895, 1030, 1281, 1410
BtsIMutI CAGTG 1 cut(s) 1428
Cac8I GCNNGC 3 cut(s) 593, 1068, 1264
CaiI CAGNNNCTG 1 cut(s) 352
CfoI GCGC 2 cut(s) 136, 913
Cfr13I GGNCC 3 cut(s) 166, 555, 664
ClaI ATCGAT 1 cut(s) 362
CseI GACGC 2 cut(s) 128, 1059
CsiI ACCWGGT 1 cut(s) 264
Csp6I GTAC 4 cut(s) 519, 575, 1020, 1491
CviAII CATG 7 cut(s) 328, 419, 788, 1016, 1067, 1218, 1258
CviQI GTAC 4 cut(s) 519, 575, 1020, 1491
DdeI CTNAG 4 cut(s) 487, 570, 596, 714
DpnI GATC 5 cut(s) 320, 456, 642, 1194, 1305
DpnII GATC 5 cut(s) 318, 454, 640, 1192, 1303
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
EciI GGCGGA 1 cut(s) 325
Ecl136II GAGCTC 2 cut(s) 461, 1266
Eco147I AGGCCT 1 cut(s) 301
Eco24I GRGCYC 2 cut(s) 463, 1268
Eco31I GGTCTC 1 cut(s) 472
Eco47I GGWCC 2 cut(s) 166, 555
Eco53kI GAGCTC 2 cut(s) 461, 1266
EcoICRI GAGCTC 2 cut(s) 461, 1266
EcoO109I RGGNCCY 1 cut(s) 555
EcoRI GAATTC 3 cut(s) 250, 491, 736
EcoRII CCWGG 3 cut(s) 264, 1400, 1464
EcoT38I GRGCYC 2 cut(s) 463, 1268
FaeI CATG 7 cut(s) 331, 422, 791, 1019, 1070, 1221, 1261
FatI CATG 7 cut(s) 327, 418, 787, 1015, 1066, 1217, 1257
Fnu4HI GCNGC 4 cut(s) 201, 204, 450, 870
FokI GGATG 5 cut(s) 870, 902, 1037, 1288, 1417
FriOI GRGCYC 2 cut(s) 463, 1268
Fsp4HI GCNGC 4 cut(s) 201, 204, 450, 870
FspBI CTAG 3 cut(s) 5, 1074, 1374
GlaI GCGC 2 cut(s) 135, 912
GluI GCNGC 4 cut(s) 201, 204, 450, 870
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 3 cut(s) 80, 301, 665
HapII CCGG 5 cut(s) 191, 221, 278, 887, 1430
HgaI GACGC 2 cut(s) 128, 1059
HhaI GCGC 2 cut(s) 136, 913
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 7 cut(s) 331, 422, 791, 1019, 1070, 1221, 1261
Hin6I GCGC 2 cut(s) 134, 911
HinP1I GCGC 2 cut(s) 134, 911
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 9 cut(s) 143, 313, 349, 530, 688, 776, 1102, 1169, 1244
HpaII CCGG 5 cut(s) 191, 221, 278, 887, 1430
HphI GGTGA 3 cut(s) 280, 464, 695
Hpy188I TCNGA 9 cut(s) 46, 57, 318, 323, 354, 490, 529, 541, 588
Hpy188III TCNNGA 6 cut(s) 221, 478, 598, 887, 1122, 1145
Hpy99I CGWCG 5 cut(s) 20, 119, 122, 182, 385
HpyAV CCTTC 2 cut(s) 166, 1440
HpyCH4III ACNGT 3 cut(s) 376, 1423, 1453
HpyCH4IV ACGT 1 cut(s) 900
HpyCH4V TGCA 4 cut(s) 403, 409, 628, 787
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 119, 244, 307, 458, 1263
HpyF3I CTNAG 4 cut(s) 487, 570, 596, 714
HpySE526I ACGT 1 cut(s) 900
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 7 cut(s) 331, 422, 791, 1019, 1070, 1221, 1261
HspAI GCGC 2 cut(s) 134, 911
Kpn2I TCCGGA 2 cut(s) 220, 886
Kzo9I GATC 5 cut(s) 318, 454, 640, 1192, 1303
LmnI GCTCC 2 cut(s) 223, 1284
Lsp1109I GCAGC 2 cut(s) 461, 856
LweI GCATC 2 cut(s) 781, 1266
MabI ACCWGGT 1 cut(s) 264
MaeI CTAG 3 cut(s) 5, 1074, 1374
MaeII ACGT 1 cut(s) 900
MaeIII GTNAC 3 cut(s) 442, 658, 1369
MalI GATC 5 cut(s) 320, 456, 642, 1194, 1305
MboI GATC 5 cut(s) 318, 454, 640, 1192, 1303
MboII GAAGA 9 cut(s) 88, 164, 283, 446, 732, 1007, 1048, 1164, 1376
MfeI CAATTG 2 cut(s) 404, 549
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 2 cut(s) 463, 1268
MlyI GAGTC 3 cut(s) 307, 343, 1253
MmeI TCCRAC 1 cut(s) 346
MroI TCCGGA 2 cut(s) 220, 886
MroXI GAANNNNTTC 1 cut(s) 51
MseI TTAA 6 cut(s) 233, 803, 903, 1094, 1437, 1475
MslI CAYNNNNRTG 1 cut(s) 888
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 5 cut(s) 191, 221, 278, 887, 1430
MspR9I CCNGG 4 cut(s) 266, 1402, 1431, 1466
MunI CAATTG 2 cut(s) 404, 549
MvaI CCWGG 3 cut(s) 266, 1402, 1466
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 6 cut(s) 77, 119, 244, 307, 458, 1263
NciI CCSGG 1 cut(s) 1431
NdeII GATC 5 cut(s) 318, 454, 640, 1192, 1303
NlaIII CATG 7 cut(s) 331, 422, 791, 1019, 1070, 1221, 1261
NlaIV GGNNCC 4 cut(s) 320, 556, 1045, 1428
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 2 cut(s) 442, 1369
NspI RCATGY 1 cut(s) 1070
NspV TTCGAA 1 cut(s) 26
PaeI GCATGC 1 cut(s) 1070
PceI AGGCCT 1 cut(s) 301
PcsI WCGNNNNNNNCGW 2 cut(s) 203, 380
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 6 cut(s) 143, 530, 688, 776, 1102, 1169
PflMI CCANNNNNTGG 1 cut(s) 1288
PkrI GCNGC 4 cut(s) 202, 205, 451, 871
PleI GAGTC 3 cut(s) 307, 343, 1252
PpsI GAGTC 3 cut(s) 307, 343, 1252
PpuMI RGGWCCY 1 cut(s) 555
Psp124BI GAGCTC 2 cut(s) 463, 1268
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 3 cut(s) 264, 1400, 1464
PspFI CCCAGC 1 cut(s) 848
PspGI CCWGG 3 cut(s) 264, 1400, 1464
PspN4I GGNNCC 4 cut(s) 320, 556, 1045, 1428
PspPI GGNCC 3 cut(s) 166, 555, 664
PspPPI RGGWCCY 1 cut(s) 555
PstNI CAGNNNCTG 1 cut(s) 352
PsuI RGATCY 1 cut(s) 318
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 4 cut(s) 520, 576, 1021, 1492
RsaNI GTAC 4 cut(s) 519, 575, 1020, 1491
RseI CAYNNNNRTG 1 cut(s) 888
SacI GAGCTC 2 cut(s) 463, 1268
SaqAI TTAA 6 cut(s) 233, 803, 903, 1094, 1437, 1475
SatI GCNGC 4 cut(s) 201, 204, 450, 870
Sau3AI GATC 5 cut(s) 318, 454, 640, 1192, 1303
Sau96I GGNCC 3 cut(s) 166, 555, 664
SchI GAGTC 3 cut(s) 307, 343, 1253
ScrFI CCNGG 4 cut(s) 266, 1402, 1431, 1466
SduI GDGCHC 2 cut(s) 463, 1268
SexAI ACCWGGT 1 cut(s) 264
SfaNI GCATC 2 cut(s) 781, 1266
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 2 cut(s) 166, 555
SmiMI CAYNNNNRTG 1 cut(s) 888
SmlI CTYRAG 1 cut(s) 1061
SmoI CTYRAG 1 cut(s) 1061
SphI GCATGC 1 cut(s) 1070
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 4 cut(s) 200, 203, 310, 670
SspMI CTAG 3 cut(s) 5, 1074, 1374
SstI GAGCTC 2 cut(s) 463, 1268
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 4 cut(s) 264, 1400, 1429, 1464
TaaI ACNGT 3 cut(s) 376, 1423, 1453
TaiI ACGT 1 cut(s) 903
TatI WGTACW 1 cut(s) 1490
TauI GCSGC 2 cut(s) 203, 206
TfiI GAWTC 6 cut(s) 143, 530, 688, 776, 1102, 1169
Tru1I TTAA 6 cut(s) 233, 803, 903, 1094, 1437, 1475
Tru9I TTAA 6 cut(s) 233, 803, 903, 1094, 1437, 1475
TscAI CASTG 1 cut(s) 1428
TseFI GTSAC 2 cut(s) 442, 1369
TseI GCWGC 2 cut(s) 449, 869
Tsp45I GTSAC 2 cut(s) 442, 1369
TspDTI ATGAA 6 cut(s) 344, 348, 669, 872, 1206, 1421
TspGWI ACGGA 3 cut(s) 179, 751, 1037
TspRI CASTG 1 cut(s) 1428
Van91I CCANNNNNTGG 1 cut(s) 1288
VpaK11BI GGWCC 2 cut(s) 166, 555
XapI RAATTY 6 cut(s) 59, 250, 491, 736, 999, 1115
XceI RCATGY 1 cut(s) 1070
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 3 cut(s) 5, 1074, 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.