Rw1G018750

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014567
Physical Location & Seq
Forward (+)
41254342 .. 41255836
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018750.1

Sequence Viewer

Length: 1461 bp
ATGCTGGAAATGGATGGCTCTTCTAAACCTCCCCAAATCTCTGAGATGTTTTCCAAATTCGCTCTCGCTTTCAAAACCAAAACCTTTGACTTATCTCTGCTCGACTCCGCCGAAGAAATCATCACCGACCAGAAGGTCGTCGTCATCAAACCCGACGGAGCACCCAGTCCGACCTCGCCGGAGCTCCAAATAATACCGACGAAGCCCAATTTGGGCGCGACCCAGGTCCGAAGCCCAGAGCTTTCGACGATTACAAGGTCTCTGAGCAAAACCCAGATCAGACCCATCAATATTCCGATGACCCAGACTCTGATTTCGTCGGTCTTCGCCACCGTTTCGTCTTTCGAAGCTTCCTACCTCCAGTTACAGACGGCCCATGTGCCCTTTGTGGAAGAAAACGTGACGTCAGCGGACCGAGCTCTGGTCTCTCACCTTCAGAGGCTCTCGGAATTCAAGCAGTTTTTCAGGGATTTTTGTGGAAGTTCGGATATCGGGTCGGGTTTTGGAGTCGGGTCTTGCTTGGAGGCCCAGGTGCAAGAGAACCAGAGCAAGCTCAGGACTTTGGGGACCATGTCAAATAGGTTGCAGACAGAGATTGATCACAAAGACAATGAAGTTGTGGCCCTGAGGAAAAAACTGGGTGAGATTCAGAAATCTAACTTTAGACTATCTCAGAGGTTATCTGGAACTTTGAATTCTTCTTCTTGTGAGGTTTTGTTGTCAGTTAGAGTATTTGATTCGGTTCTACATGATGCTTGTAGATTAACACACAGATTTAGTAAGATTTTGATAAGTTTGATGGGAAAAGCAGGGTGGGATTTGGATTTGGCTGCTAATTTGGTTCATCCTGATGTTGGTTATGTGAAGAAGGCTCATAATCGATATGCGTTTTTGTCATATGTTTGTTTGGGAATGTTCAAAGGTTTTGATTCGAAAGATTTTGGTTTAGTTGGGGATGAGGTTTTGTGTAATGGGCATGGTTCGGAATTGGATAAGAACAATGTTCCTCTGAAGCAGTTGCTTGAGCATGTTTCAAGCAATCCGATGGAGTTGTTGAGTAGGAACCAGAATTGTGAGTTTTCGAGATTTTGTGAGAGCAAGTATCAAGAGATCATTCACCCCACTATGGAATCTTCAATGTTCAGTAATTTTGATCGGGATGCAGTTGTGTTGAGTAAATGGAGGTCGTTGAGCGTATTCTATGAATCATTTGTTAACATGGCTAGCTCGATATGGACGTTACACAAGTTGGCCTATTGCTTCGATCCTGTGGTGGAGATATTCCAAGTGGAAAGAGATGCAGATTTTTCTATGGTATATATGGAAGATGTCACTAGGAGATTGTCATTGCCTAGTAAAACCAGGGAAAAAGTTGGCTTCACAGTGGTTCCGGGTTTTAAACTTGGAAGGACGATAATTCAGTCTCAGGTTTACCTAAGTGGCTTGAAATGTACAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

486

Amino Acids

54.49

Weight (kDa)

6.62

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 100 - 226 5.2e-38 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 427 - 478 2e-18 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60680
fragaria_vesca FvH4_7g11240
malus_domestica MD02G1208300.v1.1 MD07G1117100.v1.1
prunus_persica Prupe.2G140200_v2.0.a1
pyrus_communis pycom02g17060 pycom07g10760
rosa_chinensis RchiOBHm_Chr1g0349381
rosa_laevigata RLG00000028553
rosa_multiflora Rmu_sc0004208.1_g000009
rosa_roxburghii Rroxscaffold_4G00305590
rosa_rugosa Rorug01G0203100
rosa_samantha Rh1AG220500 Rh1BG186500 Rh1CG205600 Rh1DG216000
rosa_wichuraiana Rw1G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AatII GACGTC 1 cut(s) 407
AccII CGCG 1 cut(s) 218
AciI CCGC 2 cut(s) 108, 410
AclWI GGATC 1 cut(s) 1259
AcsI RAATTY 3 cut(s) 56, 449, 694
AcuI CTGAAG 2 cut(s) 419, 1031
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 1 cut(s) 1453
AfiI CCNNNNNNNGG 4 cut(s) 212, 421, 854, 1126
AgsI TTSAA 7 cut(s) 73, 454, 694, 919, 1035, 1137, 1447
AjnI CCWGG 3 cut(s) 222, 528, 1361
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 6 cut(s) 184, 241, 350, 419, 553, 1227
AluI AGCT 6 cut(s) 184, 241, 350, 419, 553, 1227
Alw21I GWGCWC 3 cut(s) 163, 186, 421
Alw26I GTCTC 3 cut(s) 264, 430, 1428
AlwI GGATC 1 cut(s) 1259
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 4 cut(s) 372, 525, 621, 1251
ApeKI GCWGC 1 cut(s) 830
ApoI RAATTY 3 cut(s) 56, 449, 694
ArsI GACNNNNNNTTYG 2 cut(s) 298, 330
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 6 cut(s) 226, 373, 412, 526, 567, 622
AsuC2I CCSGG 1 cut(s) 1392
AsuHPI GGTGA 4 cut(s) 115, 422, 653, 1109
AsuII TTCGAA 2 cut(s) 345, 932
AsuNHI GCTAGC 1 cut(s) 1223
AvaII GGWCC 3 cut(s) 226, 412, 567
AxyI CCTNAGG 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 384
BanII GRGCYC 2 cut(s) 186, 421
BbsI GAAGAC 1 cut(s) 316
Bbv12I GWGCWC 3 cut(s) 163, 186, 421
BbvI GCAGC 1 cut(s) 817
BccI CCATC 4 cut(s) 8, 293, 793, 1039
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 3 cut(s) 224, 530, 1363
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 1 cut(s) 1392
BcoDI GTCTC 3 cut(s) 264, 430, 1428
BfaI CTAG 3 cut(s) 1224, 1335, 1353
BisI GCNGC 1 cut(s) 831
BlsI GCNGC 1 cut(s) 832
Bme1390I CCNGG 4 cut(s) 224, 530, 1363, 1392
Bme18I GGWCC 3 cut(s) 226, 412, 567
BmgT120I GGNCC 6 cut(s) 226, 373, 412, 526, 567, 622
BmiI GGNNCC 3 cut(s) 568, 1064, 1389
BmrFI CCNGG 4 cut(s) 224, 530, 1363, 1392
BmrI ACTGGG 2 cut(s) 159, 647
BmsI GCATC 3 cut(s) 742, 1150, 1288
BmtI GCTAGC 1 cut(s) 1227
BmuI ACTGGG 2 cut(s) 159, 647
BoxI GACNNNNGTC 1 cut(s) 224
BpiI GAAGAC 1 cut(s) 316
BpmI CTGGAG 1 cut(s) 344
Bpu10I CCTNAGC 1 cut(s) 554
Bpu14I TTCGAA 2 cut(s) 345, 932
BpuEI CTTGAG 1 cut(s) 1043
BpuMI CCSGG 1 cut(s) 1392
Bsa29I ATCGAT 1 cut(s) 880
BsaHI GRCGYC 1 cut(s) 404
BsaI GGTCTC 2 cut(s) 264, 430
BsaJI CCNNGG 3 cut(s) 222, 528, 1362
Bsc4I CCNNNNNNNGG 4 cut(s) 212, 421, 854, 1126
Bse1I ACTGG 3 cut(s) 165, 361, 642
Bse21I CCTNAGG 1 cut(s) 626
Bse3DI GCAATG 1 cut(s) 1346
BseBI CCWGG 3 cut(s) 224, 530, 1363
BseCI ATCGAT 1 cut(s) 880
BseDI CCNNGG 3 cut(s) 222, 528, 1362
BseGI GGATG 4 cut(s) 19, 844, 961, 1165
BseLI CCNNNNNNNGG 4 cut(s) 212, 421, 854, 1126
BseMI GCAATG 1 cut(s) 1346
BseMII CTCAG 6 cut(s) 33, 254, 568, 617, 686, 1439
BseNI ACTGG 3 cut(s) 165, 361, 642
BseSI GKGCMC 1 cut(s) 384
BseXI GCAGC 1 cut(s) 817
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 4 cut(s) 374, 527, 623, 1253
BshVI ATCGAT 1 cut(s) 880
BsiHKAI GWGCWC 3 cut(s) 163, 186, 421
BsiSI CCGG 2 cut(s) 179, 1391
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 4 cut(s) 212, 421, 854, 1126
BsmAI GTCTC 3 cut(s) 264, 430, 1428
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 4 cut(s) 374, 527, 623, 1253
Bso31I GGTCTC 2 cut(s) 264, 430
Bsp119I TTCGAA 2 cut(s) 345, 932
Bsp1286I GDGCHC 4 cut(s) 163, 186, 384, 421
Bsp1407I TGTACA 1 cut(s) 1451
Bsp143I GATC 5 cut(s) 276, 598, 1110, 1153, 1264
BspACI CCGC 2 cut(s) 108, 410
BspANI GGCC 4 cut(s) 374, 527, 623, 1253
BspCNI CTCAG 6 cut(s) 34, 255, 567, 618, 685, 1438
BspDI ATCGAT 1 cut(s) 880
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 3 cut(s) 568, 1064, 1389
BspOI GCTAGC 1 cut(s) 1227
BspPI GGATC 1 cut(s) 1259
BspQI GCTCTTC 1 cut(s) 25
BspT104I TTCGAA 2 cut(s) 345, 932
BspTNI GGTCTC 2 cut(s) 264, 430
BsrDI GCAATG 1 cut(s) 1346
BsrGI TGTACA 1 cut(s) 1451
BsrI ACTGG 3 cut(s) 165, 361, 642
BssECI CCNNGG 3 cut(s) 222, 528, 1362
BssMI GATC 5 cut(s) 276, 598, 1110, 1153, 1264
BssNI GRCGYC 1 cut(s) 404
Bst2UI CCWGG 3 cut(s) 224, 530, 1363
Bst4CI ACNGT 2 cut(s) 334, 1384
Bst6I CTCTTC 1 cut(s) 25
BstACI GRCGYC 1 cut(s) 404
BstAUI TGTACA 1 cut(s) 1451
BstBI TTCGAA 2 cut(s) 345, 932
BstC8I GCNNGC 2 cut(s) 551, 1225
BstDEI CTNAG 7 cut(s) 42, 263, 554, 626, 672, 1425, 1436
BstF5I GGATG 4 cut(s) 19, 844, 961, 1165
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 5 cut(s) 279, 601, 1113, 1156, 1267
BstMAI GTCTC 3 cut(s) 264, 430, 1428
BstMBI GATC 5 cut(s) 276, 598, 1110, 1153, 1264
BstMWI GCNNNNNNNGC 1 cut(s) 416
BstNI CCWGG 3 cut(s) 224, 530, 1363
BstNSI RCATGY 1 cut(s) 1031
BstPAI GACNNNNGTC 1 cut(s) 224
BstSCI CCNGG 4 cut(s) 222, 528, 1361, 1390
BstSLI GKGCMC 1 cut(s) 384
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 817
BstV2I GAAGAC 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 880
Bsu36I CCTNAGG 1 cut(s) 626
BsuRI GGCC 4 cut(s) 374, 527, 623, 1253
BsuTUI ATCGAT 1 cut(s) 880
BtsCI GGATG 4 cut(s) 19, 844, 961, 1165
BtsIMutI CAGTG 1 cut(s) 1389
Cac8I GCNNGC 2 cut(s) 551, 1225
CaiI CAGNNNCTG 1 cut(s) 310
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 6 cut(s) 226, 373, 412, 526, 567, 622
ClaI ATCGAT 1 cut(s) 880
CpoI CGGWCCG 1 cut(s) 412
Csp6I GTAC 1 cut(s) 1452
CspI CGGWCCG 1 cut(s) 412
CviAII CATG 6 cut(s) 377, 571, 749, 977, 1028, 1219
CviQI GTAC 1 cut(s) 1452
DdeI CTNAG 7 cut(s) 42, 263, 554, 626, 672, 1425, 1436
DpnI GATC 5 cut(s) 278, 600, 1112, 1155, 1266
DpnII GATC 5 cut(s) 276, 598, 1110, 1153, 1264
DraI TTTAAA 1 cut(s) 1399
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 2 cut(s) 184, 419
Eco24I GRGCYC 2 cut(s) 186, 421
Eco31I GGTCTC 2 cut(s) 264, 430
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 3 cut(s) 226, 412, 567
Eco53kI GAGCTC 2 cut(s) 184, 419
Eco57I CTGAAG 2 cut(s) 419, 1031
Eco81I CCTNAGG 1 cut(s) 626
EcoICRI GAGCTC 2 cut(s) 184, 419
EcoRI GAATTC 2 cut(s) 449, 694
EcoRII CCWGG 3 cut(s) 222, 528, 1361
EcoRV GATATC 1 cut(s) 490
EcoT38I GRGCYC 2 cut(s) 186, 421
FaeI CATG 6 cut(s) 380, 574, 752, 980, 1031, 1222
FaqI GGGAC 1 cut(s) 580
FatI CATG 6 cut(s) 376, 570, 748, 976, 1027, 1218
FauNDI CATATG 1 cut(s) 898
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 1 cut(s) 831
FokI GGATG 4 cut(s) 26, 831, 968, 1172
FriOI GRGCYC 2 cut(s) 186, 421
Fsp4HI GCNGC 1 cut(s) 831
FspBI CTAG 3 cut(s) 1224, 1335, 1353
GlaI GCGC 1 cut(s) 217
GluI GCNGC 1 cut(s) 831
GsuI CTGGAG 1 cut(s) 344
HaeIII GGCC 4 cut(s) 374, 527, 623, 1253
HapII CCGG 2 cut(s) 179, 1391
HhaI GCGC 1 cut(s) 218
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 6 cut(s) 380, 574, 752, 980, 1031, 1222
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 8 cut(s) 104, 307, 507, 646, 737, 929, 1130, 1205
HpaI GTTAAC 1 cut(s) 1216
HpaII CCGG 2 cut(s) 179, 1391
HphI GGTGA 4 cut(s) 115, 422, 653, 1109
Hpy166II GTNNAC 2 cut(s) 1216, 1432
Hpy188III TCNNGA 6 cut(s) 556, 684, 848, 1083, 1106, 1157
Hpy8I GTNNAC 2 cut(s) 1216, 1432
Hpy99I CGWCG 5 cut(s) 143, 158, 202, 250, 322
HpyAV CCTTC 4 cut(s) 127, 443, 862, 1401
HpyCH4III ACNGT 2 cut(s) 334, 1384
HpyCH4IV ACGT 3 cut(s) 399, 404, 1238
HpyCH4V TGCA 4 cut(s) 535, 586, 1163, 1301
HpyF10VI GCNNNNNNNGC 1 cut(s) 416
HpyF3I CTNAG 7 cut(s) 42, 263, 554, 626, 672, 1425, 1436
HpySE526I ACGT 3 cut(s) 399, 404, 1238
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 6 cut(s) 380, 574, 752, 980, 1031, 1222
HspAI GCGC 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 598
KspAI GTTAAC 1 cut(s) 1216
Kzo9I GATC 5 cut(s) 276, 598, 1110, 1153, 1264
LguI GCTCTTC 1 cut(s) 25
LmnI GCTCC 3 cut(s) 158, 181, 189
Lsp1109I GCAGC 1 cut(s) 817
LweI GCATC 3 cut(s) 742, 1150, 1288
MaeI CTAG 3 cut(s) 1224, 1335, 1353
MaeII ACGT 3 cut(s) 399, 404, 1238
MaeIII GTNAC 4 cut(s) 363, 400, 1239, 1330
MalI GATC 5 cut(s) 278, 600, 1112, 1155, 1266
MboI GATC 5 cut(s) 276, 598, 1110, 1153, 1264
MboII GAAGA 9 cut(s) 12, 125, 316, 404, 690, 693, 877, 1125, 1337
MhlI GDGCHC 4 cut(s) 163, 186, 384, 421
MluCI AATT 9 cut(s) 56, 208, 449, 694, 835, 986, 1069, 1147, 1416
MlyI GAGTC 3 cut(s) 98, 301, 516
MmeI TCCRAC 1 cut(s) 194
MseI TTAA 3 cut(s) 764, 1215, 1398
MslI CAYNNNNRTG 1 cut(s) 849
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 2 cut(s) 179, 1391
MspR9I CCNGG 4 cut(s) 224, 530, 1363, 1392
MvaI CCWGG 3 cut(s) 224, 530, 1363
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 416
NciI CCSGG 1 cut(s) 1392
NdeI CATATG 1 cut(s) 898
NdeII GATC 5 cut(s) 276, 598, 1110, 1153, 1264
NheI GCTAGC 1 cut(s) 1223
NlaIII CATG 6 cut(s) 380, 574, 752, 980, 1031, 1222
NlaIV GGNNCC 3 cut(s) 568, 1064, 1389
NmuCI GTSAC 2 cut(s) 400, 1330
NspI RCATGY 1 cut(s) 1031
NspV TTCGAA 2 cut(s) 345, 932
PciSI GCTCTTC 1 cut(s) 25
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 1235
PfeI GAWTC 5 cut(s) 646, 737, 929, 1130, 1205
PflFI GACNNNGTC 1 cut(s) 571
PkrI GCNGC 1 cut(s) 832
PleI GAGTC 3 cut(s) 98, 301, 515
PpsI GAGTC 3 cut(s) 98, 301, 515
PshAI GACNNNNGTC 1 cut(s) 224
Psp124BI GAGCTC 2 cut(s) 186, 421
Psp6I CCWGG 3 cut(s) 222, 528, 1361
PspGI CCWGG 3 cut(s) 222, 528, 1361
PspN4I GGNNCC 3 cut(s) 568, 1064, 1389
PspPI GGNCC 6 cut(s) 226, 373, 412, 526, 567, 622
PstNI CAGNNNCTG 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 571
RsaI GTAC 1 cut(s) 1453
RsaNI GTAC 1 cut(s) 1452
RseI CAYNNNNRTG 1 cut(s) 849
Rsr2I CGGWCCG 1 cut(s) 412
RsrII CGGWCCG 1 cut(s) 412
SacI GAGCTC 2 cut(s) 186, 421
SapI GCTCTTC 1 cut(s) 25
SaqAI TTAA 3 cut(s) 764, 1215, 1398
SatI GCNGC 1 cut(s) 831
Sau3AI GATC 5 cut(s) 276, 598, 1110, 1153, 1264
Sau96I GGNCC 6 cut(s) 226, 373, 412, 526, 567, 622
SchI GAGTC 3 cut(s) 98, 301, 516
ScrFI CCNGG 4 cut(s) 224, 530, 1363, 1392
SduI GDGCHC 4 cut(s) 163, 186, 384, 421
SfaNI GCATC 3 cut(s) 742, 1150, 1288
SfuI TTCGAA 2 cut(s) 345, 932
SinI GGWCC 3 cut(s) 226, 412, 567
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 1 cut(s) 1022
SmoI CTYRAG 1 cut(s) 1022
Sse9I AATT 9 cut(s) 56, 208, 449, 694, 835, 986, 1069, 1147, 1416
SsiI CCGC 2 cut(s) 108, 410
SspI AATATT 1 cut(s) 292
SspMI CTAG 3 cut(s) 1224, 1335, 1353
SstI GAGCTC 2 cut(s) 186, 421
StyD4I CCNGG 4 cut(s) 222, 528, 1361, 1390
TaaI ACNGT 2 cut(s) 334, 1384
TaiI ACGT 3 cut(s) 402, 407, 1241
TaqI TCGA 8 cut(s) 102, 245, 345, 880, 932, 1082, 1229, 1263
TaqII GACCGA 2 cut(s) 310, 429
TasI AATT 9 cut(s) 56, 208, 449, 694, 835, 986, 1069, 1147, 1416
TatI WGTACW 1 cut(s) 1451
TfiI GAWTC 5 cut(s) 646, 737, 929, 1130, 1205
Tru1I TTAA 3 cut(s) 764, 1215, 1398
Tru9I TTAA 3 cut(s) 764, 1215, 1398
TscAI CASTG 1 cut(s) 1389
TseFI GTSAC 2 cut(s) 400, 1330
TseI GCWGC 1 cut(s) 830
Tsp45I GTSAC 2 cut(s) 400, 1330
TspDTI ATGAA 3 cut(s) 627, 833, 1218
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 1 cut(s) 1389
Tth111I GACNNNGTC 1 cut(s) 571
VpaK11BI GGWCC 3 cut(s) 226, 412, 567
XapI RAATTY 3 cut(s) 56, 449, 694
XceI RCATGY 1 cut(s) 1031
XspI CTAG 3 cut(s) 1224, 1335, 1353
ZraI GACGTC 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.