AT4G00080

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017953
Physical Location & Seq
Forward (+)
32748 .. 33756
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00080.1

Sequence Viewer

Length: 630 bp
ATGGCGGCTAACAACAAATTGTTCTTTGTATTACTCTCCCTGTTCCCTCTCATCATCTTCTCGGCAACCGCCACGTCATCAAAAGACTACGACACGAAGGCTTACGTGCACTCATGGTGCCGAACAACCTTATACCCGAAACTATGCGTCCGGTCGATGTCTCGTTACGTGCGTTCACGCGCGGTGCAAAATCCACGGGATCTGGCCCGATTTGCGCTCAAAGCCAGCCTATATAGAGCTAAGTACACAAAGGCGTTCTTGTTAAAAGAGGTTAAGAACTTGGAGACGACGTTGAGGCCCCAATACTACGCGTCGGTTCACGACTGCTTGGACCAAATAAGAGACAGCGTGAACCAGTTGAGTCTAGCCATAGCGGAGCTAGACAGAGTCAGCAGGAGACAGGGAAAGAGCCAAGGGGATCTACACTGGCATATAAATAACCTGCAGACGTGGACAAGCACAGCTCTTACAGACGCCGAGACTTGCGTGAGCCAGTTCCCGGGACGTAGGATGAGCAAACTTAAGGCTACGATTAAAGGAAAAGTGAAGAATGTGGAGGAAACAACTAGTAATGCCCTTGCCTTCATCGAGCACTACGCTGCTGCTAGGTACCGAGCCAGACGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

209

Amino Acids

23.97

Weight (kDa)

10.04

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 194 4.9e-33 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00080
fragaria_vesca FvH4_7g11390
prunus_persica Prupe.2G141800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349671
rosa_laevigata RLG00000028536
rosa_roxburghii Rroxscaffold_4G00305390
rosa_rugosa Rorug01G0204900
rosa_samantha Rh1AG222500 Rh1BG188500 Rh1DG217700
rosa_wichuraiana Rw1G018970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 625
Acc36I ACCTGC 1 cut(s) 450
Acc65I GGTACC 1 cut(s) 609
AccB1I GGYRCC 2 cut(s) 117, 609
AccII CGCG 3 cut(s) 180, 182, 311
AciI CCGC 4 cut(s) 5, 69, 182, 374
AclWI GGATC 2 cut(s) 207, 426
AcyI GRCGYC 2 cut(s) 474, 622
AfaI GTAC 2 cut(s) 245, 611
AfiI CCNNNNNNNGG 1 cut(s) 499
AflII CTTAAG 1 cut(s) 521
AflIII ACRYGT 1 cut(s) 309
AhlI ACTAGT 1 cut(s) 566
AjiI CACGTC 2 cut(s) 75, 450
AluBI AGCT 3 cut(s) 239, 379, 464
AluI AGCT 3 cut(s) 239, 379, 464
Alw21I GWGCWC 2 cut(s) 111, 594
Alw26I GTCTC 5 cut(s) 165, 278, 336, 391, 473
Alw44I GTGCAC 1 cut(s) 107
AlwI GGATC 2 cut(s) 207, 426
Ama87I CYCGRG 1 cut(s) 499
AoxI GGCC 2 cut(s) 204, 296
ApaLI GTGCAC 1 cut(s) 107
ApeKI GCWGC 2 cut(s) 599, 602
Asp718I GGTACC 1 cut(s) 609
AspLEI GCGC 2 cut(s) 182, 217
AspS9I GGNCC 3 cut(s) 205, 297, 331
AsuC2I CCSGG 2 cut(s) 500, 501
AvaI CYCGRG 1 cut(s) 499
AvaII GGWCC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 111
BanI GGYRCC 2 cut(s) 117, 609
Bbv12I GWGCWC 2 cut(s) 111, 594
BbvI GCAGC 2 cut(s) 586, 589
BcnI CCSGG 2 cut(s) 500, 501
BcoDI GTCTC 5 cut(s) 165, 278, 336, 391, 473
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 4 cut(s) 365, 380, 567, 606
BfmI CTRYAG 1 cut(s) 443
BfrI CTTAAG 1 cut(s) 521
BfuAI ACCTGC 1 cut(s) 450
BisI GCNGC 3 cut(s) 6, 600, 603
BlsI GCNGC 3 cut(s) 7, 601, 604
Bme1390I CCNGG 2 cut(s) 500, 501
Bme18I GGWCC 1 cut(s) 331
BmeT110I CYCGRG 1 cut(s) 499
BmgBI CACGTC 2 cut(s) 75, 450
BmgT120I GGNCC 3 cut(s) 205, 297, 331
BmiI GGNNCC 3 cut(s) 119, 299, 611
BmrFI CCNGG 2 cut(s) 500, 501
BpuMI CCSGG 2 cut(s) 500, 501
BsaAI YACGTR 2 cut(s) 106, 169
BsaHI GRCGYC 2 cut(s) 474, 622
BsaJI CCNNGG 3 cut(s) 194, 412, 499
BsaWI WCCGGW 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse1I ACTGG 3 cut(s) 355, 431, 493
BseDI CCNNGG 3 cut(s) 194, 412, 499
BseGI GGATG 1 cut(s) 516
BseLI CCNNNNNNNGG 1 cut(s) 499
BseNI ACTGG 3 cut(s) 355, 431, 493
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 2 cut(s) 586, 589
Bsh1236I CGCG 3 cut(s) 180, 182, 311
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 206, 298
BshNI GGYRCC 2 cut(s) 117, 609
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 111, 594
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 2 cut(s) 151, 500
BslFI GGGAC 1 cut(s) 516
BslI CCNNNNNNNGG 1 cut(s) 499
BsmAI GTCTC 5 cut(s) 165, 278, 336, 391, 473
BsmBI CGTCTC 1 cut(s) 278
BsmFI GGGAC 1 cut(s) 516
BsnI GGCC 2 cut(s) 206, 298
BsoBI CYCGRG 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 111, 594
Bsp143I GATC 2 cut(s) 199, 418
BspACI CCGC 4 cut(s) 5, 69, 182, 374
BspANI GGCC 2 cut(s) 206, 298
BspFNI CGCG 3 cut(s) 180, 182, 311
BspLI GGNNCC 3 cut(s) 119, 299, 611
BspMAI CTGCAG 1 cut(s) 447
BspMI ACCTGC 1 cut(s) 450
BspPI GGATC 2 cut(s) 207, 426
BspT107I GGYRCC 2 cut(s) 117, 609
BspTI CTTAAG 1 cut(s) 521
BsrI ACTGG 3 cut(s) 355, 431, 493
BssECI CCNNGG 3 cut(s) 194, 412, 499
BssMI GATC 2 cut(s) 199, 418
BssNI GRCGYC 2 cut(s) 474, 622
BssT1I CCWWGG 1 cut(s) 412
BstACI GRCGYC 2 cut(s) 474, 622
BstAFI CTTAAG 1 cut(s) 521
BstBAI YACGTR 2 cut(s) 106, 169
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 194
BstF5I GGATG 1 cut(s) 516
BstFNI CGCG 3 cut(s) 180, 182, 311
BstHHI GCGC 2 cut(s) 182, 217
BstKTI GATC 2 cut(s) 202, 421
BstMAI GTCTC 5 cut(s) 165, 278, 336, 391, 473
BstMBI GATC 2 cut(s) 199, 418
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 2 cut(s) 212, 221
BstSCI CCNGG 2 cut(s) 498, 499
BstSFI CTRYAG 1 cut(s) 443
BstSLI GKGCMC 1 cut(s) 111
BstUI CGCG 3 cut(s) 180, 182, 311
BstV1I GCAGC 2 cut(s) 586, 589
BstX2I RGATCY 2 cut(s) 199, 418
BstYI RGATCY 2 cut(s) 199, 418
BsuRI GGCC 2 cut(s) 206, 298
BtgI CCRYGG 1 cut(s) 194
BtrI CACGTC 2 cut(s) 75, 450
BtsCI GGATG 1 cut(s) 516
BtsIMutI CAGTG 1 cut(s) 424
BveI ACCTGC 1 cut(s) 450
Cac8I GCNNGC 1 cut(s) 226
CfoI GCGC 2 cut(s) 182, 217
Cfr13I GGNCC 3 cut(s) 205, 297, 331
Cfr9I CCCGGG 1 cut(s) 499
CseI GACGC 3 cut(s) 136, 300, 482
Csp6I GTAC 2 cut(s) 244, 610
CviAII CATG 1 cut(s) 114
CviQI GTAC 2 cut(s) 244, 610
DdeI CTNAG 1 cut(s) 240
DpnI GATC 2 cut(s) 201, 420
DpnII GATC 2 cut(s) 199, 418
Eco130I CCWWGG 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 331
Eco88I CYCGRG 1 cut(s) 499
EcoO109I RGGNCCY 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 412
Esp3I CGTCTC 1 cut(s) 278
FaeI CATG 1 cut(s) 117
FaiI YATR 8 cut(s) 115, 133, 145, 232, 234, 371, 432, 434
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 1 cut(s) 516
FatI CATG 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 6, 600, 603
FokI GGATG 1 cut(s) 523
Fsp4HI GCNGC 3 cut(s) 6, 600, 603
FspBI CTAG 4 cut(s) 365, 380, 567, 606
GlaI GCGC 2 cut(s) 181, 216
GluI GCNGC 3 cut(s) 6, 600, 603
HaeIII GGCC 2 cut(s) 206, 298
HapII CCGG 2 cut(s) 151, 500
HgaI GACGC 3 cut(s) 136, 300, 482
HhaI GCGC 2 cut(s) 182, 217
Hin1I GRCGYC 2 cut(s) 474, 622
Hin1II CATG 1 cut(s) 117
Hin6I GCGC 2 cut(s) 180, 215
HinP1I GCGC 2 cut(s) 180, 215
HinfI GANTC 2 cut(s) 361, 387
HpaII CCGG 2 cut(s) 151, 500
Hpy166II GTNNAC 6 cut(s) 109, 176, 246, 319, 352, 453
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 6 cut(s) 109, 176, 246, 319, 352, 453
Hpy99I CGWCG 2 cut(s) 292, 316
HpyAV CCTTC 2 cut(s) 91, 592
HpyCH4IV ACGT 7 cut(s) 74, 105, 168, 290, 449, 505, 622
HpyCH4V TGCA 3 cut(s) 109, 187, 445
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 221
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 7 cut(s) 74, 105, 168, 290, 449, 505, 622
Hsp92I GRCGYC 2 cut(s) 474, 622
Hsp92II CATG 1 cut(s) 117
HspAI GCGC 2 cut(s) 180, 215
KpnI GGTACC 1 cut(s) 613
Kzo9I GATC 2 cut(s) 199, 418
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 2 cut(s) 586, 589
MaeI CTAG 4 cut(s) 365, 380, 567, 606
MaeII ACGT 7 cut(s) 74, 105, 168, 290, 449, 505, 622
MaeIII GTNAC 1 cut(s) 164
MalI GATC 2 cut(s) 201, 420
MboI GATC 2 cut(s) 199, 418
MboII GAAGA 2 cut(s) 49, 559
MflI RGATCY 2 cut(s) 199, 418
MhlI GDGCHC 2 cut(s) 111, 594
MluCI AATT 1 cut(s) 17
MluI ACGCGT 1 cut(s) 309
MlyI GAGTC 2 cut(s) 370, 396
MnlI CCTC 4 cut(s) 57, 262, 288, 550
MseI TTAA 5 cut(s) 263, 273, 522, 534, 628
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 2 cut(s) 151, 500
MspR9I CCNGG 2 cut(s) 500, 501
MvnI CGCG 3 cut(s) 180, 182, 311
MwoI GCNNNNNNNGC 2 cut(s) 212, 221
NciI CCSGG 2 cut(s) 500, 501
NdeII GATC 2 cut(s) 199, 418
NlaIII CATG 1 cut(s) 117
NlaIV GGNNCC 3 cut(s) 119, 299, 611
NmeAIII GCCGAG 2 cut(s) 41, 502
PcsI WCGNNNNNNNCGW 1 cut(s) 169
PflFI GACNNNGTC 1 cut(s) 386
PkrI GCNGC 3 cut(s) 7, 601, 604
PleI GAGTC 2 cut(s) 369, 395
PpsI GAGTC 2 cut(s) 369, 395
Ppu21I YACGTR 2 cut(s) 106, 169
PspN4I GGNNCC 3 cut(s) 119, 299, 611
PspPI GGNCC 3 cut(s) 205, 297, 331
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 2 cut(s) 199, 418
PsyI GACNNNGTC 1 cut(s) 386
RsaI GTAC 2 cut(s) 245, 611
RsaNI GTAC 2 cut(s) 244, 610
SaqAI TTAA 5 cut(s) 263, 273, 522, 534, 628
SatI GCNGC 3 cut(s) 6, 600, 603
Sau3AI GATC 2 cut(s) 199, 418
Sau96I GGNCC 3 cut(s) 205, 297, 331
SchI GAGTC 2 cut(s) 370, 396
ScrFI CCNGG 2 cut(s) 500, 501
SduI GDGCHC 2 cut(s) 111, 594
SfcI CTRYAG 1 cut(s) 443
SinI GGWCC 1 cut(s) 331
SmaI CCCGGG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SpeI ACTAGT 1 cut(s) 566
Sse9I AATT 1 cut(s) 17
SsiI CCGC 4 cut(s) 5, 69, 182, 374
SspMI CTAG 4 cut(s) 365, 380, 567, 606
StyD4I CCNGG 2 cut(s) 498, 499
StyI CCWWGG 1 cut(s) 412
TaiI ACGT 7 cut(s) 77, 108, 171, 293, 452, 508, 625
TaqI TCGA 2 cut(s) 155, 588
TasI AATT 1 cut(s) 17
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 5 cut(s) 263, 273, 522, 534, 628
Tru9I TTAA 5 cut(s) 263, 273, 522, 534, 628
TscAI CASTG 1 cut(s) 431
TseI GCWGC 2 cut(s) 599, 602
TspDTI ATGAA 1 cut(s) 574
TspMI CCCGGG 1 cut(s) 499
TspRI CASTG 1 cut(s) 431
Tth111I GACNNNGTC 1 cut(s) 386
Vha464I CTTAAG 1 cut(s) 521
VneI GTGCAC 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 331
XmaI CCCGGG 1 cut(s) 499
XspI CTAG 4 cut(s) 365, 380, 567, 606
ZraI GACGTC 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.