Prupe.2G141800_v2.0.a1

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0017953
Physical Location & Seq
Reverse (-)
19788161 .. 19788802
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G141800.1

Sequence Viewer

Length: 642 bp
ATGGCCAAATTCAGCTTCTCCTTGCTGCTCATCCTCCTCTCTATTCTCTGCCTTTTGAGCAGTGTTGAGCCTTCCTTTGCTAGCCATCCTCAGCAGCCTCGACGTTCCAAGGCCCGGGCCTACATCGAGGCCTCGTGCCGGACCACCCGTTACCCAAAGTTATGCGTTCACTGCCTCTCCACCTACATCAAGCCCAACATGACCATCCAAAGCCCACAGCAGCTGGCCCAAGTTGCCTTGTCCGTGAGCCTGTACCGGGCCCAGTACGCAAGAGCATATGTCCTCAAGGTGACAAGAGAGCTCAAGGAACTGAAGGCCAGAGAATACCAAGTTGTGCATGATTGCTTGAACCAGATCAATGATAGTGTGGCCCAACTCAGTGAGTCCATTAAAGAACTACGTCGTTTAGGGCAAGCAACTGTGGGTGATGACTTGTTTTGGCACATAAGCAATGTTGAGACTTGGGTGAGCACAGCTTTAACTGATGCATACACTTGCGTGGATGAGCTTCCAGGGAAAAACATGAGCAAGTTGAAGGCCACCATTAAAGGGAAGGTGCTGAATGTGGCTGAGGTCACCAGCAATGCTCTGACTTTGTTTCATAGATTTGCTCAAAGGTACCGAGCTGCAAAGAAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

214

Amino Acids

24.08

Weight (kDa)

9.62

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00080
fragaria_vesca FvH4_7g11390
prunus_persica Prupe.2G141800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349671
rosa_laevigata RLG00000028536
rosa_roxburghii Rroxscaffold_4G00305390
rosa_rugosa Rorug01G0204900
rosa_samantha Rh1AG222500 Rh1BG188500 Rh1DG217700
rosa_wichuraiana Rw1G018970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 618
AccB1I GGYRCC 1 cut(s) 618
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 332
AfaI GTAC 3 cut(s) 254, 266, 620
AfiI CCNNNNNNNGG 4 cut(s) 114, 138, 256, 549
AgsI TTSAA 2 cut(s) 349, 535
AjnI CCWGG 1 cut(s) 511
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 6 cut(s) 15, 223, 301, 476, 508, 626
AluI AGCT 6 cut(s) 15, 223, 301, 476, 508, 626
Alw21I GWGCWC 2 cut(s) 303, 473
Alw26I GTCTC 1 cut(s) 452
AlwNI CAGNNNCTG 1 cut(s) 223
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 9 cut(s) 3, 111, 117, 129, 225, 258, 315, 369, 537
ApaI GGGCCC 1 cut(s) 262
ApeKI GCWGC 4 cut(s) 25, 94, 220, 626
ApoI RAATTY 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 618
AspS9I GGNCC 7 cut(s) 112, 117, 141, 226, 258, 259, 370
AsuC2I CCSGG 3 cut(s) 115, 116, 257
AsuHPI GGTGA 4 cut(s) 301, 437, 478, 568
AsuNHI GCTAGC 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 114
AvaII GGWCC 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 262
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 618
BanII GRGCYC 2 cut(s) 262, 303
BauI CACGAG 1 cut(s) 133
Bbv12I GWGCWC 2 cut(s) 303, 473
BbvCI CCTCAGC 2 cut(s) 90, 570
BbvI GCAGC 4 cut(s) 12, 106, 232, 613
BccI CCATC 2 cut(s) 93, 212
BciT130I CCWGG 1 cut(s) 513
BcnI CCSGG 3 cut(s) 115, 116, 257
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 1 cut(s) 81
BisI GCNGC 4 cut(s) 26, 95, 221, 627
BlsI GCNGC 4 cut(s) 27, 96, 222, 628
Bme1390I CCNGG 4 cut(s) 115, 116, 257, 513
Bme18I GGWCC 1 cut(s) 141
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 7 cut(s) 112, 117, 141, 226, 258, 259, 370
BmiI GGNNCC 2 cut(s) 260, 620
BmrFI CCNGG 4 cut(s) 115, 116, 257, 513
BmrI ACTGGG 1 cut(s) 256
BmsI GCATC 1 cut(s) 475
BmtI GCTAGC 1 cut(s) 84
BmuI ACTGGG 1 cut(s) 256
Bpu10I CCTNAGC 2 cut(s) 90, 570
BpuEI CTTGAG 2 cut(s) 269, 287
BpuMI CCSGG 3 cut(s) 115, 116, 257
BsaJI CCNNGG 3 cut(s) 108, 114, 512
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 4 cut(s) 114, 138, 256, 549
Bse1I ACTGG 1 cut(s) 262
Bse3DI GCAATG 2 cut(s) 457, 589
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 3 cut(s) 108, 114, 512
BseGI GGATG 4 cut(s) 30, 85, 204, 508
BseLI CCNNNNNNNGG 4 cut(s) 114, 138, 256, 549
BseMI GCAATG 2 cut(s) 457, 589
BseMII CTCAG 3 cut(s) 104, 391, 561
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 262
BseXI GCAGC 4 cut(s) 12, 106, 232, 613
BshFI GGCC 9 cut(s) 5, 113, 119, 131, 227, 260, 317, 371, 539
BshNI GGYRCC 1 cut(s) 618
BsiHKAI GWGCWC 2 cut(s) 303, 473
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 3 cut(s) 115, 139, 256
BslI CCNNNNNNNGG 4 cut(s) 114, 138, 256, 549
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 9 cut(s) 5, 113, 119, 131, 227, 260, 317, 371, 539
BsoBI CYCGRG 1 cut(s) 114
Bsp120I GGGCCC 1 cut(s) 258
Bsp1286I GDGCHC 3 cut(s) 262, 303, 473
Bsp143I GATC 1 cut(s) 354
BspANI GGCC 9 cut(s) 5, 113, 119, 131, 227, 260, 317, 371, 539
BspCNI CTCAG 3 cut(s) 103, 390, 562
BspLI GGNNCC 2 cut(s) 260, 620
BspOI GCTAGC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 618
BsrDI GCAATG 2 cut(s) 457, 589
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 3 cut(s) 108, 114, 512
BssMI GATC 1 cut(s) 354
BssSI CACGAG 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 108
Bst2BI CACGAG 1 cut(s) 133
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 1 cut(s) 421
BstC8I GCNNGC 3 cut(s) 82, 225, 414
BstDEI CTNAG 3 cut(s) 90, 377, 570
BstEII GGTNACC 1 cut(s) 574
BstF5I GGATG 4 cut(s) 30, 85, 204, 508
BstKTI GATC 1 cut(s) 357
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 1 cut(s) 354
BstMWI GCNNNNNNNGC 4 cut(s) 57, 171, 233, 266
BstNI CCWGG 1 cut(s) 513
BstPI GGTNACC 1 cut(s) 574
BstSCI CCNGG 4 cut(s) 113, 114, 255, 511
BstSLI GKGCMC 1 cut(s) 262
BstV1I GCAGC 4 cut(s) 12, 106, 232, 613
BsuRI GGCC 9 cut(s) 5, 113, 119, 131, 227, 260, 317, 371, 539
BtsCI GGATG 4 cut(s) 30, 85, 204, 508
BtsI GCAGTG 2 cut(s) 67, 169
BtsIMutI CAGTG 3 cut(s) 67, 169, 385
Cac8I GCNNGC 3 cut(s) 82, 225, 414
CaiI CAGNNNCTG 1 cut(s) 223
Cfr13I GGNCC 7 cut(s) 112, 117, 141, 226, 258, 259, 370
Cfr9I CCCGGG 1 cut(s) 114
Csp6I GTAC 3 cut(s) 253, 265, 619
CviAII CATG 3 cut(s) 199, 338, 523
CviQI GTAC 3 cut(s) 253, 265, 619
DdeI CTNAG 3 cut(s) 90, 377, 570
DpnI GATC 1 cut(s) 356
DpnII GATC 1 cut(s) 354
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 2 cut(s) 262, 303
Eco47I GGWCC 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 114
Eco91I GGTNACC 1 cut(s) 574
EcoICRI GAGCTC 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 574
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 490
EcoT38I GRGCYC 2 cut(s) 262, 303
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 3 cut(s) 202, 341, 526
FaiI YATR 9 cut(s) 163, 200, 277, 279, 339, 446, 490, 524, 603
FatI CATG 3 cut(s) 198, 337, 522
FauNDI CATATG 1 cut(s) 277
Fnu4HI GCNGC 4 cut(s) 26, 95, 221, 627
FokI GGATG 4 cut(s) 17, 72, 191, 515
FriOI GRGCYC 2 cut(s) 262, 303
Fsp4HI GCNGC 4 cut(s) 26, 95, 221, 627
FspBI CTAG 1 cut(s) 81
GluI GCNGC 4 cut(s) 26, 95, 221, 627
HaeIII GGCC 9 cut(s) 5, 113, 119, 131, 227, 260, 317, 371, 539
HapII CCGG 3 cut(s) 115, 139, 256
Hin1II CATG 3 cut(s) 202, 341, 526
HinfI GANTC 1 cut(s) 383
HpaII CCGG 3 cut(s) 115, 139, 256
HphI GGTGA 4 cut(s) 301, 437, 478, 568
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 1 cut(s) 591
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 2 cut(s) 105, 405
HpyAV CCTTC 4 cut(s) 81, 307, 529, 547
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4IV ACGT 2 cut(s) 103, 400
HpyCH4V TGCA 3 cut(s) 337, 488, 629
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 171, 233, 266
HpyF3I CTNAG 3 cut(s) 90, 377, 570
HpySE526I ACGT 2 cut(s) 103, 400
Hsp92II CATG 3 cut(s) 202, 341, 526
KpnI GGTACC 1 cut(s) 622
Kzo9I GATC 1 cut(s) 354
Lsp1109I GCAGC 4 cut(s) 12, 106, 232, 613
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 81
MaeII ACGT 2 cut(s) 103, 400
MaeIII GTNAC 3 cut(s) 149, 289, 574
MalI GATC 1 cut(s) 356
MboI GATC 1 cut(s) 354
MhlI GDGCHC 3 cut(s) 262, 303, 473
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 8
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 392
MnlI CCTC 9 cut(s) 44, 47, 99, 108, 121, 142, 185, 293, 565
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 490
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 390, 479, 546
MslI CAYNNNNRTG 1 cut(s) 497
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 3 cut(s) 115, 139, 256
MspR9I CCNGG 4 cut(s) 115, 116, 257, 513
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 57, 171, 233, 266
NciI CCSGG 3 cut(s) 115, 116, 257
NdeI CATATG 1 cut(s) 277
NdeII GATC 1 cut(s) 354
NheI GCTAGC 1 cut(s) 80
NlaIII CATG 3 cut(s) 202, 341, 526
NlaIV GGNNCC 2 cut(s) 260, 620
NmuCI GTSAC 2 cut(s) 289, 574
NsiI ATGCAT 1 cut(s) 490
OliI CACNNNNGTG 1 cut(s) 497
PceI AGGCCT 1 cut(s) 131
PkrI GCNGC 4 cut(s) 27, 96, 222, 628
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 511
PspEI GGTNACC 1 cut(s) 574
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 2 cut(s) 260, 620
PspOMI GGGCCC 1 cut(s) 258
PspPI GGNCC 7 cut(s) 112, 117, 141, 226, 258, 259, 370
PstNI CAGNNNCTG 1 cut(s) 223
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 3 cut(s) 254, 266, 620
RsaNI GTAC 3 cut(s) 253, 265, 619
RseI CAYNNNNRTG 1 cut(s) 497
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 3 cut(s) 390, 479, 546
SatI GCNGC 4 cut(s) 26, 95, 221, 627
Sau3AI GATC 1 cut(s) 354
Sau96I GGNCC 7 cut(s) 112, 117, 141, 226, 258, 259, 370
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 4 cut(s) 115, 116, 257, 513
SduI GDGCHC 3 cut(s) 262, 303, 473
SfaNI GCATC 1 cut(s) 475
SinI GGWCC 1 cut(s) 141
SmaI CCCGGG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 284, 302
SmoI CTYRAG 2 cut(s) 284, 302
SrfI GCCCGGGC 1 cut(s) 116
Sse9I AATT 1 cut(s) 8
SseBI AGGCCT 1 cut(s) 131
SspMI CTAG 1 cut(s) 81
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 4 cut(s) 113, 114, 255, 511
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 421
TaiI ACGT 2 cut(s) 106, 403
TaqI TCGA 2 cut(s) 100, 126
TasI AATT 1 cut(s) 8
Tru1I TTAA 3 cut(s) 390, 479, 546
Tru9I TTAA 3 cut(s) 390, 479, 546
TscAI CASTG 3 cut(s) 67, 176, 385
TseFI GTSAC 2 cut(s) 289, 574
TseI GCWGC 4 cut(s) 25, 94, 220, 626
Tsp45I GTSAC 2 cut(s) 289, 574
TspDTI ATGAA 1 cut(s) 590
TspGWI ACGGA 1 cut(s) 232
TspMI CCCGGG 1 cut(s) 114
TspRI CASTG 3 cut(s) 67, 176, 385
VpaK11BI GGWCC 1 cut(s) 141
XapI RAATTY 1 cut(s) 8
XmaI CCCGGG 1 cut(s) 114
XspI CTAG 1 cut(s) 81
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.