RLG00000028536

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017953
Physical Location & Seq
Forward (+)
24011328 .. 24012966
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028536

Sequence Viewer

Length: 795 bp
ATGACACCTTCGGGACCCCAGATGAGCCCCTTGAAACCATCACAGGAATTAAGAACCTTGCTTCCCATTCGGAATCATCATGACACCTTCGGGACCCCAGATGTGCCCCTTGAAACCATCACAGGAATTAAGAACCTTGCTAACCAAAAAAAAATGGCCAAACTTTGCCTTTCTTTGCTGCTAATCCTCCTTTCCATCCTCTCCCTTTCGAGCACCGTGGAGCCAGCCTTTTCGGGACATACTGCTCGACGTTCTAGGGCTCGAGCCAACATCGAAGCCGCGTGCCGGACCACCCGCTACCCGGACCTATGCGTCCACTGCCTCACCACCTACGTCAAACCCAACATGACCATCCAAAGCCCCCAGCAACTTGCCCAAGTCGCCTTGACTGCAAGCCTCTACCGTGCCCTATATGCTAGGGCGTTCGTGCTCAAGGTAGCAAAAGAGATTAACACACTGAAGAGCGAAAACCAAGTTGTCCGAGATTGCTTGCAGCAGATAAACGATAGCGTGGACCAACTCAGTGGATCGATCAAGGAACTACGTCGTTTGGGGCAAGAATCTGTGGGTGATGACCTGTTTTGGCACATAAGCAACGTTGAGACGTGGGTTGGCACTGCTCTAACTGATGCAAACGATTGTGTAGATGCCCTTCCGGGACCAAACATGAGCAAACTGAAGGCGACTATTAAAGGAAAGGTGCTCAATGTGGCTCAGACGGCTAGTAATGCACTGGCTTTGTTTCATAGTTTTGCTGCAAGATATAAAGCTACCAGTGCCACCAAGAAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

265

Amino Acids

28.91

Weight (kDa)

9.51

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 90 - 247 3.9e-32 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00080
fragaria_vesca FvH4_7g11390
prunus_persica Prupe.2G141800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349671
rosa_laevigata RLG00000028536
rosa_roxburghii Rroxscaffold_4G00305390
rosa_rugosa Rorug01G0204900
rosa_samantha Rh1AG222500 Rh1BG188500 Rh1DG217700
rosa_wichuraiana Rw1G018970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AccII CGCG 1 cut(s) 281
AciI CCGC 2 cut(s) 279, 295
AclI AACGTT 1 cut(s) 597
AclWI GGATC 1 cut(s) 535
AcoI YGGCCR 1 cut(s) 156
AcuI CTGAAG 2 cut(s) 479, 698
AfiI CCNNNNNNNGG 2 cut(s) 285, 301
AgsI TTSAA 2 cut(s) 34, 113
AjiI CACGTC 1 cut(s) 606
AloI GAACNNNNNNTCC 2 cut(s) 46, 78
AluBI AGCT 1 cut(s) 770
AluI AGCT 1 cut(s) 770
Alw21I GWGCWC 3 cut(s) 215, 432, 705
Alw26I GTCTC 1 cut(s) 596
AlwI GGATC 1 cut(s) 535
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 3 cut(s) 178, 493, 755
AspS9I GGNCC 6 cut(s) 14, 93, 288, 304, 514, 659
AsuC2I CCSGG 2 cut(s) 302, 657
AsuHPI GGTGA 2 cut(s) 316, 581
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 6 cut(s) 14, 93, 288, 304, 514, 659
BaeGI GKGCMC 2 cut(s) 108, 409
BalI TGGCCA 1 cut(s) 158
BanII GRGCYC 2 cut(s) 29, 262
BarI GAAGNNNNNNTAC 2 cut(s) 636, 668
Bbv12I GWGCWC 3 cut(s) 215, 432, 705
BbvI GCAGC 3 cut(s) 165, 505, 742
BccI CCATC 4 cut(s) 46, 125, 203, 359
BceAI ACGGC 1 cut(s) 735
BcnI CCSGG 2 cut(s) 302, 657
BcoDI GTCTC 1 cut(s) 596
BfaI CTAG 4 cut(s) 255, 417, 723, 793
BisI GCNGC 4 cut(s) 179, 279, 494, 756
BlsI GCNGC 4 cut(s) 180, 280, 495, 757
Bme1390I CCNGG 2 cut(s) 302, 657
Bme18I GGWCC 6 cut(s) 14, 93, 288, 304, 514, 659
BmeT110I CYCGRG 1 cut(s) 261
BmgBI CACGTC 1 cut(s) 606
BmgT120I GGNCC 6 cut(s) 14, 93, 288, 304, 514, 659
BmiI GGNNCC 6 cut(s) 15, 16, 94, 95, 222, 660
BmrFI CCNGG 2 cut(s) 302, 657
BmsI GCATC 2 cut(s) 619, 637
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 2 cut(s) 302, 657
Bsa29I ATCGAT 1 cut(s) 530
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 301
Bse1I ACTGG 2 cut(s) 738, 774
BseCI ATCGAT 1 cut(s) 530
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 2 cut(s) 195, 351
BseLI CCNNNNNNNGG 2 cut(s) 285, 301
BseMII CTCAG 2 cut(s) 535, 728
BseNI ACTGG 2 cut(s) 738, 774
BseSI GKGCMC 2 cut(s) 108, 409
BseXI GCAGC 3 cut(s) 165, 505, 742
BseYI CCCAGC 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 1 cut(s) 158
BshVI ATCGAT 1 cut(s) 530
BsiHKAI GWGCWC 3 cut(s) 215, 432, 705
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 3 cut(s) 286, 302, 656
BslFI GGGAC 4 cut(s) 27, 106, 249, 672
BslI CCNNNNNNNGG 2 cut(s) 285, 301
BsmAI GTCTC 1 cut(s) 596
BsmBI CGTCTC 1 cut(s) 596
BsmFI GGGAC 4 cut(s) 27, 106, 249, 672
BsnI GGCC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 7 cut(s) 29, 108, 215, 262, 409, 432, 705
Bsp143I GATC 2 cut(s) 527, 531
BspACI CCGC 2 cut(s) 279, 295
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 2 cut(s) 534, 727
BspDI ATCGAT 1 cut(s) 530
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 6 cut(s) 15, 16, 94, 95, 222, 660
BspPI GGATC 1 cut(s) 535
BspQI GCTCTTC 1 cut(s) 455
BsrI ACTGG 2 cut(s) 738, 774
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 2 cut(s) 527, 531
Bst4CI ACNGT 2 cut(s) 217, 404
Bst6I CTCTTC 1 cut(s) 455
BstC8I GCNNGC 4 cut(s) 225, 283, 394, 491
BstDEI CTNAG 2 cut(s) 521, 714
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 2 cut(s) 195, 351
BstFNI CGCG 1 cut(s) 281
BstKTI GATC 2 cut(s) 530, 534
BstMAI GTCTC 1 cut(s) 596
BstMBI GATC 2 cut(s) 527, 531
BstMWI GCNNNNNNNGC 7 cut(s) 318, 380, 389, 413, 719, 728, 776
BstSCI CCNGG 2 cut(s) 300, 655
BstSLI GKGCMC 2 cut(s) 108, 409
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 3 cut(s) 165, 505, 742
BstXI CCANNNNNNTGG 1 cut(s) 524
Bsu15I ATCGAT 1 cut(s) 530
BsuRI GGCC 1 cut(s) 158
BsuTUI ATCGAT 1 cut(s) 530
BtgI CCRYGG 1 cut(s) 216
BtrI CACGTC 1 cut(s) 606
BtsCI GGATG 2 cut(s) 195, 351
BtsI GCAGTG 2 cut(s) 316, 615
BtsIMutI CAGTG 6 cut(s) 316, 455, 529, 615, 731, 781
Cac8I GCNNGC 4 cut(s) 225, 283, 394, 491
CciI TCATGA 1 cut(s) 79
Cfr13I GGNCC 6 cut(s) 14, 93, 288, 304, 514, 659
ClaI ATCGAT 1 cut(s) 530
CseI GACGC 1 cut(s) 301
CviAII CATG 3 cut(s) 80, 346, 667
DdeI CTNAG 2 cut(s) 521, 714
DpnI GATC 2 cut(s) 529, 533
DpnII GATC 2 cut(s) 527, 531
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Eco24I GRGCYC 2 cut(s) 29, 262
Eco47I GGWCC 6 cut(s) 14, 93, 288, 304, 514, 659
Eco57I CTGAAG 2 cut(s) 479, 698
Eco88I CYCGRG 1 cut(s) 261
EcoO109I RGGNCCY 2 cut(s) 14, 93
EcoT38I GRGCYC 2 cut(s) 29, 262
Esp3I CGTCTC 1 cut(s) 596
FaeI CATG 3 cut(s) 83, 349, 670
FaqI GGGAC 4 cut(s) 27, 106, 249, 672
FatI CATG 3 cut(s) 79, 345, 666
FauI CCCGC 1 cut(s) 302
Fnu4HI GCNGC 4 cut(s) 179, 279, 494, 756
FokI GGATG 2 cut(s) 182, 338
FriOI GRGCYC 2 cut(s) 29, 262
Fsp4HI GCNGC 4 cut(s) 179, 279, 494, 756
FspBI CTAG 4 cut(s) 255, 417, 723, 793
GluI GCNGC 4 cut(s) 179, 279, 494, 756
GsaI CCCAGC 1 cut(s) 367
HaeIII GGCC 1 cut(s) 158
HapII CCGG 3 cut(s) 286, 302, 656
HgaI GACGC 1 cut(s) 301
Hin1II CATG 3 cut(s) 83, 349, 670
HinfI GANTC 2 cut(s) 73, 560
HpaII CCGG 3 cut(s) 286, 302, 656
HphI GGTGA 2 cut(s) 316, 581
Hpy166II GTNNAC 2 cut(s) 316, 514
Hpy188I TCNGA 3 cut(s) 72, 482, 717
Hpy188III TCNNGA 4 cut(s) 12, 80, 91, 234
Hpy8I GTNNAC 2 cut(s) 316, 514
Hpy99I CGWCG 2 cut(s) 252, 549
HpyAV CCTTC 4 cut(s) 18, 97, 662, 673
HpyCH4III ACNGT 2 cut(s) 217, 404
HpyCH4IV ACGT 5 cut(s) 250, 333, 544, 597, 605
HpyCH4V TGCA 5 cut(s) 392, 493, 632, 731, 758
HpyF10VI GCNNNNNNNGC 7 cut(s) 318, 380, 389, 413, 719, 728, 776
HpyF3I CTNAG 2 cut(s) 521, 714
HpySE526I ACGT 5 cut(s) 250, 333, 544, 597, 605
Hsp92II CATG 3 cut(s) 83, 349, 670
KflI GGGWCCC 2 cut(s) 14, 93
Kzo9I GATC 2 cut(s) 527, 531
LguI GCTCTTC 1 cut(s) 455
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 3 cut(s) 165, 505, 742
LweI GCATC 2 cut(s) 619, 637
MaeI CTAG 4 cut(s) 255, 417, 723, 793
MaeII ACGT 5 cut(s) 250, 333, 544, 597, 605
MalI GATC 2 cut(s) 529, 533
MboI GATC 2 cut(s) 527, 531
MboII GAAGA 1 cut(s) 472
MhlI GDGCHC 7 cut(s) 29, 108, 215, 262, 409, 432, 705
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 2 cut(s) 47, 126
MluNI TGGCCA 1 cut(s) 158
MnlI CCTC 4 cut(s) 197, 209, 332, 407
Mox20I TGGCCA 1 cut(s) 158
MscI TGGCCA 1 cut(s) 158
MseI TTAA 4 cut(s) 50, 129, 450, 690
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 3 cut(s) 286, 302, 656
MspR9I CCNGG 2 cut(s) 302, 657
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 7 cut(s) 318, 380, 389, 413, 719, 728, 776
NciI CCSGG 2 cut(s) 302, 657
NdeII GATC 2 cut(s) 527, 531
NlaIII CATG 3 cut(s) 83, 349, 670
NlaIV GGNNCC 6 cut(s) 15, 16, 94, 95, 222, 660
PaeR7I CTCGAG 1 cut(s) 261
PagI TCATGA 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 455
PfeI GAWTC 2 cut(s) 73, 560
PfoI TCCNGGA 1 cut(s) 655
PkrI GCNGC 4 cut(s) 180, 280, 495, 757
PpuMI RGGWCCY 2 cut(s) 14, 93
Psp1406I AACGTT 1 cut(s) 597
Psp5II RGGWCCY 2 cut(s) 14, 93
PspFI CCCAGC 1 cut(s) 363
PspN4I GGNNCC 6 cut(s) 15, 16, 94, 95, 222, 660
PspPI GGNCC 6 cut(s) 14, 93, 288, 304, 514, 659
PspPPI RGGWCCY 2 cut(s) 14, 93
PspXI VCTCGAGB 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 4 cut(s) 50, 129, 450, 690
SatI GCNGC 4 cut(s) 179, 279, 494, 756
Sau3AI GATC 2 cut(s) 527, 531
Sau96I GGNCC 6 cut(s) 14, 93, 288, 304, 514, 659
ScrFI CCNGG 2 cut(s) 302, 657
SduI GDGCHC 7 cut(s) 29, 108, 215, 262, 409, 432, 705
SfaNI GCATC 2 cut(s) 619, 637
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 6 cut(s) 14, 93, 288, 304, 514, 659
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 261, 431
SmoI CTYRAG 2 cut(s) 261, 431
Sse9I AATT 2 cut(s) 47, 126
SsiI CCGC 2 cut(s) 279, 295
SspMI CTAG 4 cut(s) 255, 417, 723, 793
StyD4I CCNGG 2 cut(s) 300, 655
TaaI ACNGT 2 cut(s) 217, 404
TaiI ACGT 5 cut(s) 253, 336, 547, 600, 608
TaqI TCGA 5 cut(s) 209, 247, 262, 273, 530
TasI AATT 2 cut(s) 47, 126
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 2 cut(s) 73, 560
Tru1I TTAA 4 cut(s) 50, 129, 450, 690
Tru9I TTAA 4 cut(s) 50, 129, 450, 690
TscAI CASTG 6 cut(s) 323, 462, 529, 622, 738, 781
TseI GCWGC 3 cut(s) 178, 493, 755
TspDTI ATGAA 1 cut(s) 734
TspRI CASTG 6 cut(s) 323, 462, 529, 622, 738, 781
VpaK11BI GGWCC 6 cut(s) 14, 93, 288, 304, 514, 659
XhoI CTCGAG 1 cut(s) 261
XspI CTAG 4 cut(s) 255, 417, 723, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.