Rorug01G0204900

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017953
Physical Location & Seq
Reverse (-)
28757091 .. 28760281
3191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0204900.1

Sequence Viewer

Length: 1056 bp
ATGGCTCCTCCCAGTATTCTCGGTCCTCCCGAGTTCCACAAACCCTCACCCACCACCACCGCTCCGCGCGCCACCCCAACCCAAATCCAAACCCGACTCCAAACCCACCACCGGCGAATCCTTCGTGGACCTGATGGTGGCGAATTTCAACGACGTCGGAACCAAGCACGACCTCCCAATGGGCCTAACGGAGAACGGCTCTGCCACGTTTCTCTCCACCGGCAACCCCTGCCTCGATCTCTTCTTCCACGTCATCCCAAACACTCCAGGCGACTACCTCACGTCCTCACCGATGGGACTCCAATGGTGGCCAGTGTCGTGTCACCGGATTCTTCCTTTATCGCAATCACTTCATCTTCTTCACCTTCATATTCTTCTTCGTTTTTAACGAAACTAAGGCACGCGCCGAGTCTTATTGCTATGTGCTTGCTTCCTCGAAGGCTTGGAAATCCGGTTTCAATTGCTCGCAATAGGATTGGAAATCTGGTTTCTTTCACAGAAGTTAGGATTACGAGGTTTAGGGAAATTGATATCAAAGAGGTGCCAACTTTTGCAAAAGGATCAAATTTCTCATTTTTCATTGCACATGCTGAGATTAGTATAAACCTTGTAGGAGGTGGGATCAATTCAGATAATTCTTTGCAATACATAGAAGAAGGGGCTACCCACGTCATTGTCACTTCTTATGTATTTAATAATGGGCAAAACCTTGAAAGGCTTAAAGATCTTGTTCGTGTTGTTGGAAAAGAAAGGCTTGTTTTAGACCTTAGCTGTAGAAAGAGGAAGAACATATGTATAGCTGGTTTGACTTTTGAAGATGATCCAGTCGATAAAAGTGAAAAGCTGGAACGAGATGGGAAACAATGGGTTGATATGTGCTCTTCAAGGTGTTATGTACCCAGAGTGCATGATAATGTCCTGCTGCAAAAGTGGCTATATTATGAAAATAATGTTTCTGAATACAGGGTTTGTGCATCTGGCTTCTGTTATCCTTATTCTTATCTTTTCCCATCAAGTTCTTTATATGGGCAGTTTTATTATACTGTTTTCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

351

Amino Acids

39.68

Weight (kDa)

9.67

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00080
fragaria_vesca FvH4_7g11390
prunus_persica Prupe.2G141800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349671
rosa_laevigata RLG00000028536
rosa_roxburghii Rroxscaffold_4G00305390
rosa_rugosa Rorug01G0204900
rosa_samantha Rh1AG222500 Rh1BG188500 Rh1DG217700
rosa_wichuraiana Rw1G018970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 541
AccBSI CCGCTC 1 cut(s) 62
AccII CGCG 3 cut(s) 67, 69, 404
AciI CCGC 2 cut(s) 60, 65
AclWI GGATC 3 cut(s) 568, 629, 815
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 2 cut(s) 143, 565
AcyI GRCGYC 1 cut(s) 154
AfaI GTAC 1 cut(s) 897
AfiI CCNNNNNNNGG 3 cut(s) 111, 137, 179
AgsI TTSAA 5 cut(s) 149, 459, 713, 815, 885
AjiI CACGTC 3 cut(s) 251, 283, 670
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 3 cut(s) 771, 800, 844
AluI AGCT 3 cut(s) 771, 800, 844
Alw21I GWGCWC 1 cut(s) 881
AlwI GGATC 3 cut(s) 568, 629, 815
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 2 cut(s) 182, 309
ApeKI GCWGC 1 cut(s) 922
ApoI RAATTY 2 cut(s) 143, 565
AspLEI GCGC 3 cut(s) 69, 71, 406
AspS9I GGNCC 3 cut(s) 23, 128, 182
AsuHPI GGTGA 4 cut(s) 39, 280, 315, 354
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 2 cut(s) 23, 128
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 1 cut(s) 541
Bbv12I GWGCWC 1 cut(s) 881
BbvI GCAGC 1 cut(s) 909
BccI CCATC 4 cut(s) 128, 287, 848, 1018
BceAI ACGGC 1 cut(s) 212
BciT130I CCWGG 1 cut(s) 268
BfmI CTRYAG 1 cut(s) 772
BglII AGATCT 1 cut(s) 724
BisI GCNGC 1 cut(s) 923
BlsI GCNGC 1 cut(s) 924
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 2 cut(s) 23, 128
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 3 cut(s) 251, 283, 670
BmgT120I GGNCC 3 cut(s) 23, 128, 182
BmiI GGNNCC 3 cut(s) 6, 161, 543
BmrFI CCNGG 1 cut(s) 268
BmrI ACTGGG 1 cut(s) 6
BmsI GCATC 1 cut(s) 983
BmuI ACTGGG 1 cut(s) 6
BplI GAGNNNNNCTC 2 cut(s) 183, 215
BpmI CTGGAG 1 cut(s) 250
Bpu10I CCTNAGC 1 cut(s) 767
BsaHI GRCGYC 1 cut(s) 154
BsaWI WCCGGW 2 cut(s) 325, 451
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 137, 179
Bse118I RCCGGY 2 cut(s) 111, 219
Bse1I ACTGG 3 cut(s) 12, 312, 824
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 1 cut(s) 268
BseGI GGATG 1 cut(s) 253
BseLI CCNNNNNNNGG 3 cut(s) 111, 137, 179
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 3 cut(s) 12, 312, 824
BsePI GCGCGC 1 cut(s) 67
BseXI GCAGC 1 cut(s) 909
Bsh1236I CGCG 3 cut(s) 67, 69, 404
BshFI GGCC 2 cut(s) 184, 311
BshNI GGYRCC 1 cut(s) 541
BsiHKAI GWGCWC 1 cut(s) 881
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 4 cut(s) 112, 220, 326, 452
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 3 cut(s) 111, 137, 179
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 2 cut(s) 184, 311
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 881
Bsp143I GATC 5 cut(s) 236, 560, 621, 724, 820
BspACI CCGC 2 cut(s) 60, 65
BspANI GGCC 2 cut(s) 184, 311
BspCNI CTCAG 1 cut(s) 583
BspFNI CGCG 3 cut(s) 67, 69, 404
BspLI GGNNCC 3 cut(s) 6, 161, 543
BspPI GGATC 3 cut(s) 568, 629, 815
BspQI GCTCTTC 1 cut(s) 886
BspT107I GGYRCC 1 cut(s) 541
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 579
BsrFI RCCGGY 2 cut(s) 111, 219
BsrI ACTGG 3 cut(s) 12, 312, 824
BssAI RCCGGY 2 cut(s) 111, 219
BssHII GCGCGC 1 cut(s) 67
BssMI GATC 5 cut(s) 236, 560, 621, 724, 820
BssNI GRCGYC 1 cut(s) 154
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 1045
Bst6I CTCTTC 2 cut(s) 246, 886
BstACI GRCGYC 1 cut(s) 154
BstAPI GCANNNNNTGC 1 cut(s) 229
BstC8I GCNNGC 4 cut(s) 69, 402, 428, 466
BstDEI CTNAG 3 cut(s) 395, 591, 767
BstF5I GGATG 1 cut(s) 253
BstFNI CGCG 3 cut(s) 67, 69, 404
BstHHI GCGC 3 cut(s) 69, 71, 406
BstKTI GATC 5 cut(s) 239, 563, 624, 727, 823
BstMBI GATC 5 cut(s) 236, 560, 621, 724, 820
BstMWI GCNNNNNNNGC 3 cut(s) 68, 229, 931
BstNI CCWGG 1 cut(s) 268
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 1 cut(s) 266
BstSFI CTRYAG 1 cut(s) 772
BstUI CGCG 3 cut(s) 67, 69, 404
BstV1I GCAGC 1 cut(s) 909
BstX2I RGATCY 1 cut(s) 724
BstYI RGATCY 1 cut(s) 724
BsuRI GGCC 2 cut(s) 184, 311
BtrI CACGTC 3 cut(s) 251, 283, 670
BtsCI GGATG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 319
Cac8I GCNNGC 4 cut(s) 69, 402, 428, 466
CfoI GCGC 3 cut(s) 69, 71, 406
Cfr10I RCCGGY 2 cut(s) 111, 219
Cfr13I GGNCC 3 cut(s) 23, 128, 182
Csp6I GTAC 1 cut(s) 896
CviAII CATG 2 cut(s) 587, 908
CviQI GTAC 1 cut(s) 896
DdeI CTNAG 3 cut(s) 395, 591, 767
DpnI GATC 5 cut(s) 238, 562, 623, 726, 822
DpnII GATC 5 cut(s) 236, 560, 621, 724, 820
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 2 cut(s) 246, 886
EarI CTCTTC 2 cut(s) 246, 886
Eco32I GATATC 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 23, 128
Eco88I CYCGRG 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 266
EcoRV GATATC 1 cut(s) 532
FaeI CATG 2 cut(s) 590, 911
FalI AAGNNNNNCTT 2 cut(s) 738, 770
FaqI GGGAC 1 cut(s) 310
FatI CATG 2 cut(s) 586, 907
FauNDI CATATG 1 cut(s) 791
Fnu4HI GCNGC 1 cut(s) 923
FokI GGATG 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 923
GlaI GCGC 3 cut(s) 68, 70, 405
GluI GCNGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 250
HaeIII GGCC 2 cut(s) 184, 311
HapII CCGG 4 cut(s) 112, 220, 326, 452
HhaI GCGC 3 cut(s) 69, 71, 406
Hin1I GRCGYC 1 cut(s) 154
Hin1II CATG 2 cut(s) 590, 911
Hin6I GCGC 3 cut(s) 67, 69, 404
HinP1I GCGC 3 cut(s) 67, 69, 404
HinfI GANTC 5 cut(s) 96, 117, 298, 329, 409
HpaII CCGG 4 cut(s) 112, 220, 326, 452
HphI GGTGA 4 cut(s) 39, 280, 315, 354
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 3 cut(s) 159, 631, 958
Hpy188III TCNNGA 1 cut(s) 29
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 2 cut(s) 156, 159
HpyAV CCTTC 4 cut(s) 131, 375, 432, 650
HpyCH4III ACNGT 1 cut(s) 1045
HpyCH4IV ACGT 5 cut(s) 154, 207, 250, 282, 669
HpyCH4V TGCA 6 cut(s) 554, 584, 643, 907, 925, 974
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 229, 931
HpyF3I CTNAG 3 cut(s) 395, 591, 767
HpySE526I ACGT 5 cut(s) 154, 207, 250, 282, 669
Hsp92I GRCGYC 1 cut(s) 154
Hsp92II CATG 2 cut(s) 590, 911
HspAI GCGC 3 cut(s) 67, 69, 404
Kzo9I GATC 5 cut(s) 236, 560, 621, 724, 820
LguI GCTCTTC 1 cut(s) 886
LmnI GCTCC 2 cut(s) 10, 67
Lsp1109I GCAGC 1 cut(s) 909
LweI GCATC 1 cut(s) 983
MaeII ACGT 5 cut(s) 154, 207, 250, 282, 669
MaeIII GTNAC 2 cut(s) 321, 676
MalI GATC 5 cut(s) 238, 562, 623, 726, 822
MbiI CCGCTC 1 cut(s) 62
MboI GATC 5 cut(s) 236, 560, 621, 724, 820
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 1 cut(s) 724
MhlI GDGCHC 1 cut(s) 881
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 6 cut(s) 143, 459, 525, 565, 625, 634
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 3 cut(s) 90, 292, 418
MmeI TCCRAC 2 cut(s) 137, 721
Mox20I TGGCCA 1 cut(s) 311
MscI TGGCCA 1 cut(s) 311
MseI TTAA 3 cut(s) 386, 693, 720
MslI CAYNNNNRTG 1 cut(s) 912
Msp20I TGGCCA 1 cut(s) 311
MspI CCGG 4 cut(s) 112, 220, 326, 452
MspR9I CCNGG 1 cut(s) 268
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 268
MvnI CGCG 3 cut(s) 67, 69, 404
MwoI GCNNNNNNNGC 3 cut(s) 68, 229, 931
NdeI CATATG 1 cut(s) 791
NdeII GATC 5 cut(s) 236, 560, 621, 724, 820
NlaIII CATG 2 cut(s) 590, 911
NlaIV GGNNCC 3 cut(s) 6, 161, 543
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 2 cut(s) 321, 676
NspI RCATGY 1 cut(s) 590
PauI GCGCGC 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 886
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 288, 386
PfeI GAWTC 2 cut(s) 117, 329
PkrI GCNGC 1 cut(s) 924
PleI GAGTC 3 cut(s) 90, 292, 417
PpsI GAGTC 3 cut(s) 90, 292, 417
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 3 cut(s) 6, 161, 543
PspPI GGNCC 3 cut(s) 23, 128, 182
PsuI RGATCY 1 cut(s) 724
PteI GCGCGC 1 cut(s) 67
RsaI GTAC 1 cut(s) 897
RsaNI GTAC 1 cut(s) 896
RseI CAYNNNNRTG 1 cut(s) 912
SapI GCTCTTC 1 cut(s) 886
SaqAI TTAA 3 cut(s) 386, 693, 720
SatI GCNGC 1 cut(s) 923
Sau3AI GATC 5 cut(s) 236, 560, 621, 724, 820
Sau96I GGNCC 3 cut(s) 23, 128, 182
SchI GAGTC 3 cut(s) 90, 292, 418
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 1 cut(s) 881
SfaNI GCATC 1 cut(s) 983
SfcI CTRYAG 1 cut(s) 772
SgrAI CRCCGGYG 1 cut(s) 111
SinI GGWCC 2 cut(s) 23, 128
SmiMI CAYNNNNRTG 1 cut(s) 912
Sse9I AATT 6 cut(s) 143, 459, 525, 565, 625, 634
SsiI CCGC 2 cut(s) 60, 65
StyD4I CCNGG 1 cut(s) 266
TaaI ACNGT 1 cut(s) 1045
TaiI ACGT 5 cut(s) 157, 210, 253, 285, 672
TaqI TCGA 3 cut(s) 235, 436, 828
TaqII GACCGA 1 cut(s) 11
TasI AATT 6 cut(s) 143, 459, 525, 565, 625, 634
TfiI GAWTC 2 cut(s) 117, 329
Tru1I TTAA 3 cut(s) 386, 693, 720
Tru9I TTAA 3 cut(s) 386, 693, 720
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 2 cut(s) 321, 676
TseI GCWGC 1 cut(s) 922
Tsp45I GTSAC 2 cut(s) 321, 676
TspDTI ATGAA 4 cut(s) 342, 357, 568, 957
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 1 cut(s) 319
VpaK11BI GGWCC 2 cut(s) 23, 128
XapI RAATTY 2 cut(s) 143, 565
XceI RCATGY 1 cut(s) 590
ZraI GACGTC 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.