AT4G08040

1-aminocyclopropane-1-carboxylate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014996
Physical Location & Seq
Forward (+)
4886962 .. 4889224
2263 bp
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UTR
Exon/CDS
Intron
AT4G08040.1

Sequence Viewer

Length: 1383 bp
ATGTTGTCAAGCAAAGTTGTTGGCGACTCTCATGGACAAGACTCATCCTACTTCCTTGGATGGCAAGAATACGAGAAGAATCCTTTCCACGAGTCGTTTAACACTAGTGGGATTGTTCAAATGGGTCTTGCTGAAAACCAGCTTTCTTTTGACCTAATAGAGAAATGGCTTGAAGAGCATCCAGAAGTCTTGGGTTTGAAGAAAAATGATGAGTCGGTGTTTAGACAATTAGCTCTGTTCCAAGATTACCATGGCTTGCCAGCTTTCAAGGATGCCATGGCGAAGTTCATGGGGAAAATCAGAGAGAACAAAGTGAAATTCGATACGAACAAGATGGTTCTTACAGCTGGATCAACCTCGGCTAACGAGACTCTAATGTTCTGTCTTGCTAATCCAGGAGATGCCTTTCTTATCCCTGCACCTTATTATCCAGGGTTTGATAGAGATCTCAAATGGAGGACAGGAGTAGAGATTGTTCCTATCCATTGCGTAAGCTCAAATGGGTACAAGATAACCGAGGATGCATTAGAAGATGCCTACGAACGAGCTCTCAAACATAACCTAAATGTTAAAGGAGTTCTCATAACCAACCCTTCAAACCCACTTGGAACCTCTACCACCCGTGAAGAGCTTGATCTTCTTCTGACCTTCACATCAACCAAGAAAATCCATATGGTTAGCGATGAGATCTACTCGGGAACGGTTTTCGACTCTCCTGAGTTCACCAGCGTTCTAGAAGTGGCTAAGGACAAGAACATGGGTTTAGATGGTAAAATCCATGTTGTTTACAGCTTGTCCAAAGATCTAGGCCTCCCCGGATTTCGTGTTGGCTTGATTTACTCAAACAATGAGAAAGTGGTGTCAGCCGCGACTAAAATGTCGAGTTTTGGACTCATTTCTTCCCAAACTCAACATTTGCTAGCCAATTTGCTGTCTGATGAAAGATTCACGACCAACTATTTGGAAGAGAACAAGAAGAGGCTGAGAGAGAGAAAGGATAGGCTGGTTTCGGGTCTAAAGGAAGCGGGTATCAGTTGTTTGAAGAGTAACGCAGGTTTGTTCTGTTGGGTTGACTTAAGACACCTCTTGAAATCCAACACTTTTGAGGCCGAGCATTCTTTATGGACAAAGATTGTGTGTGAAGTTGGTCTTAACATCTCTCCAGGCTCATCGTGTCATTGCGATGAACCTGGTTGGTTTAGAGTTTGTTTCGCGAATATGTCGGACCAAACGATGGAGGTTGCTATGGACCGTGTTAAAGGTTTTGTTGACAACAATAATGGTGGTAAACAAAAGAGAACCATGTGGGATACAAGGAGAAGATCTCTTATCAACAAATGGGTCTCCAAGCTTTCCTCTGTTACTTGTGAATCAGAACGTTGA

Protein Analysis

460

Amino Acids

51.8

Weight (kDa)

6.34

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 40 - 419 6.5e-104 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08040
fragaria_vesca FvH4_2g32470 FvH4_3g04180
malus_domestica MD08G1076500.v1.1 MD15G1145400.v1.1
prunus_persica Prupe.1G417800_v2.0.a1
pyrus_communis pycom08g06340
rosa_chinensis RchiOBHm_Chr6g0310191
rosa_laevigata RLG00000010501
rosa_multiflora Rmu_sc0008163.1_g000012
rosa_roxburghii Rroxscaffold_7G00158760
rosa_rugosa Rorug06G0378100
rosa_samantha Rh6BG501800 Rh6CG506800 Rh6DG492900
rosa_wichuraiana Rw6G042830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 877
Acc36I ACCTGC 1 cut(s) 1043
AccB7I CCANNNNNTGG 1 cut(s) 1234
AccII CGCG 2 cut(s) 869, 1214
AciI CCGC 2 cut(s) 867, 1025
AclI AACGTT 1 cut(s) 1378
AclWI GGATC 1 cut(s) 358
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 1234
AflII CTTAAG 1 cut(s) 1075
AgsI TTSAA 7 cut(s) 119, 173, 199, 268, 597, 1042, 1090
AhlI ACTAGT 1 cut(s) 104
AjnI CCWGG 4 cut(s) 394, 430, 1162, 1189
AluBI AGCT 9 cut(s) 142, 233, 263, 347, 495, 548, 631, 792, 1351
AluI AGCT 9 cut(s) 142, 233, 263, 347, 495, 548, 631, 792, 1351
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 2 cut(s) 362, 1348
AlwI GGATC 1 cut(s) 358
Ama87I CYCGRG 1 cut(s) 694
AoxI GGCC 2 cut(s) 808, 1107
ApoI RAATTY 1 cut(s) 317
ArsI GACNNNNNNTTYG 2 cut(s) 943, 975
Asp700I GAANNNNTTC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 1225, 1249
AsuC2I CCSGG 1 cut(s) 816
AsuHPI GGTGA 1 cut(s) 715
AsuNHI GCTAGC 1 cut(s) 919
AvaI CYCGRG 1 cut(s) 694
AvaII GGWCC 2 cut(s) 1225, 1249
BanII GRGCYC 1 cut(s) 550
BauI CACGAG 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 550
BccI CCATC 4 cut(s) 54, 328, 761, 1228
BciT130I CCWGG 4 cut(s) 396, 432, 1164, 1191
BciVI GTATCC 1 cut(s) 1303
BcnI CCSGG 1 cut(s) 816
BcoDI GTCTC 2 cut(s) 362, 1348
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 4 cut(s) 105, 734, 806, 920
BfrI CTTAAG 1 cut(s) 1075
BfuAI ACCTGC 1 cut(s) 1043
BfuI GTATCC 1 cut(s) 1303
BglII AGATCT 4 cut(s) 445, 687, 802, 1322
BisI GCNGC 1 cut(s) 867
BlsI GCNGC 1 cut(s) 868
Bme1390I CCNGG 5 cut(s) 396, 432, 816, 1164, 1191
Bme18I GGWCC 2 cut(s) 1225, 1249
BmeT110I CYCGRG 1 cut(s) 694
BmgT120I GGNCC 2 cut(s) 1225, 1249
BmiI GGNNCC 1 cut(s) 610
BmrFI CCNGG 5 cut(s) 396, 432, 816, 1164, 1191
BmsI GCATC 5 cut(s) 187, 262, 391, 511, 523
BmtI GCTAGC 1 cut(s) 923
BplI GAGNNNNNCTC 4 cut(s) 677, 709, 1309, 1341
BpmI CTGGAG 1 cut(s) 1146
Bpu10I CCTNAGC 1 cut(s) 744
BpuMI CCSGG 1 cut(s) 816
BsaBI GATNNNNATC 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 1348
BsaJI CCNNGG 7 cut(s) 55, 250, 276, 357, 431, 516, 814
Bsc4I CCNNNNNNNGG 1 cut(s) 1234
Bse3DI GCAATG 2 cut(s) 484, 1177
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 4 cut(s) 396, 432, 1164, 1191
BseDI CCNNGG 7 cut(s) 55, 250, 276, 357, 431, 516, 814
BseGI GGATG 5 cut(s) 44, 65, 178, 277, 526
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 1 cut(s) 1234
BseMI GCAATG 2 cut(s) 484, 1177
BseMII CTCAG 2 cut(s) 708, 974
BsgI GTGCAG 1 cut(s) 402
Bsh1236I CGCG 2 cut(s) 869, 1214
BshFI GGCC 2 cut(s) 810, 1109
BsiHKAI GWGCWC 1 cut(s) 550
BsiHKCI CYCGRG 1 cut(s) 694
BsiSI CCGG 1 cut(s) 816
BslI CCNNNNNNNGG 1 cut(s) 1234
BsmAI GTCTC 2 cut(s) 362, 1348
BsmI GAATGC 1 cut(s) 1114
BsnI GGCC 2 cut(s) 810, 1109
Bso31I GGTCTC 1 cut(s) 1348
BsoBI CYCGRG 1 cut(s) 694
Bsp1286I GDGCHC 1 cut(s) 550
Bsp143I GATC 6 cut(s) 350, 445, 634, 687, 802, 1322
Bsp19I CCATGG 2 cut(s) 250, 276
Bsp68I TCGCGA 1 cut(s) 1214
BspACI CCGC 2 cut(s) 867, 1025
BspANI GGCC 2 cut(s) 810, 1109
BspCNI CTCAG 2 cut(s) 709, 975
BspFNI CGCG 2 cut(s) 869, 1214
BspLI GGNNCC 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 1043
BspOI GCTAGC 1 cut(s) 923
BspPI GGATC 1 cut(s) 358
BspQI GCTCTTC 2 cut(s) 168, 621
BspTI CTTAAG 1 cut(s) 1075
BspTNI GGTCTC 1 cut(s) 1348
BsrDI GCAATG 2 cut(s) 484, 1177
BssECI CCNNGG 7 cut(s) 55, 250, 276, 357, 431, 516, 814
BssMI GATC 6 cut(s) 350, 445, 634, 687, 802, 1322
BssSI CACGAG 1 cut(s) 89
BssT1I CCWWGG 3 cut(s) 55, 250, 276
Bst2BI CACGAG 1 cut(s) 89
Bst2UI CCWGG 4 cut(s) 396, 432, 1164, 1191
Bst4CI ACNGT 2 cut(s) 703, 1253
Bst6I CTCTTC 5 cut(s) 168, 621, 960, 971, 1037
BstAFI CTTAAG 1 cut(s) 1075
BstC8I GCNNGC 3 cut(s) 257, 261, 921
BstDEI CTNAG 3 cut(s) 717, 744, 983
BstDSI CCRYGG 2 cut(s) 250, 276
BstF5I GGATG 5 cut(s) 44, 65, 178, 277, 526
BstFNI CGCG 2 cut(s) 869, 1214
BstKTI GATC 6 cut(s) 353, 448, 637, 690, 805, 1325
BstMAI GTCTC 2 cut(s) 362, 1348
BstMBI GATC 6 cut(s) 350, 445, 634, 687, 802, 1322
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 4 cut(s) 396, 432, 1164, 1191
BstSCI CCNGG 5 cut(s) 394, 430, 814, 1162, 1189
BstUI CGCG 2 cut(s) 869, 1214
BstX2I RGATCY 4 cut(s) 445, 687, 802, 1322
BstXI CCANNNNNNTGG 1 cut(s) 961
BstYI RGATCY 4 cut(s) 445, 687, 802, 1322
BsuI GTATCC 1 cut(s) 1303
BsuRI GGCC 2 cut(s) 810, 1109
BtgI CCRYGG 2 cut(s) 250, 276
BtgZI GCGATG 2 cut(s) 696, 1197
BtsCI GGATG 5 cut(s) 44, 65, 178, 277, 526
BtuMI TCGCGA 1 cut(s) 1214
BveI ACCTGC 1 cut(s) 1043
Cac8I GCNNGC 3 cut(s) 257, 261, 921
Cfr13I GGNCC 2 cut(s) 1225, 1249
CsiI ACCWGGT 1 cut(s) 1189
Csp6I GTAC 1 cut(s) 505
CspCI CAANNNNNGTGG 2 cut(s) 1264, 1299
CviAII CATG 7 cut(s) 32, 251, 277, 289, 757, 779, 1303
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 3 cut(s) 717, 744, 983
DpnI GATC 6 cut(s) 352, 447, 636, 689, 804, 1324
DpnII GATC 6 cut(s) 350, 445, 634, 687, 802, 1322
DrdI GACNNNNNNGTC 1 cut(s) 877
DseDI GACNNNNNNGTC 1 cut(s) 877
Eam1104I CTCTTC 5 cut(s) 168, 621, 960, 971, 1037
EarI CTCTTC 5 cut(s) 168, 621, 960, 971, 1037
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 55, 250, 276
Eco147I AGGCCT 1 cut(s) 810
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 1348
Eco47I GGWCC 2 cut(s) 1225, 1249
Eco53kI GAGCTC 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 694
EcoICRI GAGCTC 1 cut(s) 548
EcoRII CCWGG 4 cut(s) 394, 430, 1162, 1189
EcoT14I CCWWGG 3 cut(s) 55, 250, 276
EcoT22I ATGCAT 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 3 cut(s) 55, 250, 276
FaeI CATG 7 cut(s) 35, 254, 280, 292, 760, 782, 1306
FalI AAGNNNNNCTT 2 cut(s) 1134, 1166
FatI CATG 7 cut(s) 31, 250, 276, 288, 756, 778, 1302
FauI CCCGC 1 cut(s) 1018
FauNDI CATATG 1 cut(s) 672
Fnu4HI GCNGC 1 cut(s) 867
FokI GGATG 5 cut(s) 31, 72, 165, 284, 533
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 1 cut(s) 867
FspBI CTAG 4 cut(s) 105, 734, 806, 920
GluI GCNGC 1 cut(s) 867
GsuI CTGGAG 1 cut(s) 1146
HaeIII GGCC 2 cut(s) 810, 1109
HapII CCGG 1 cut(s) 816
Hin1II CATG 7 cut(s) 35, 254, 280, 292, 760, 782, 1306
HincII GTYRAC 2 cut(s) 1072, 1270
HindII GTYRAC 2 cut(s) 1072, 1270
HindIII AAGCTT 1 cut(s) 1349
HpaII CCGG 1 cut(s) 816
HphI GGTGA 1 cut(s) 715
Hpy166II GTNNAC 5 cut(s) 723, 787, 1072, 1270, 1289
Hpy188I TCNGA 5 cut(s) 302, 645, 937, 1225, 1375
Hpy188III TCNNGA 7 cut(s) 182, 696, 716, 734, 949, 1087, 1213
Hpy8I GTNNAC 5 cut(s) 723, 787, 1072, 1270, 1289
HpyAV CCTTC 2 cut(s) 603, 658
HpyCH4III ACNGT 2 cut(s) 703, 1253
HpyCH4IV ACGT 1 cut(s) 1378
HpyCH4V TGCA 2 cut(s) 419, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 3 cut(s) 717, 744, 983
HpySE526I ACGT 1 cut(s) 1378
Hsp92II CATG 7 cut(s) 35, 254, 280, 292, 760, 782, 1306
Kzo9I GATC 6 cut(s) 350, 445, 634, 687, 802, 1322
LguI GCTCTTC 2 cut(s) 168, 621
LweI GCATC 5 cut(s) 187, 262, 391, 511, 523
MabI ACCWGGT 1 cut(s) 1189
MaeI CTAG 4 cut(s) 105, 734, 806, 920
MaeII ACGT 1 cut(s) 1378
MaeIII GTNAC 2 cut(s) 1046, 1360
MalI GATC 6 cut(s) 352, 447, 636, 689, 804, 1324
MboI GATC 6 cut(s) 350, 445, 634, 687, 802, 1322
MflI RGATCY 4 cut(s) 445, 687, 802, 1322
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 3 cut(s) 227, 317, 925
MlyI GAGTC 7 cut(s) 20, 35, 101, 221, 364, 704, 885
MmeI TCCRAC 2 cut(s) 1119, 1203
Mph1103I ATGCAT 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 83
MseI TTAA 5 cut(s) 99, 570, 1076, 1152, 1257
MslI CAYNNNNRTG 1 cut(s) 1182
MspA1I CMGCKG 1 cut(s) 347
MspCI CTTAAG 1 cut(s) 1075
MspI CCGG 1 cut(s) 816
MspR9I CCNGG 5 cut(s) 396, 432, 816, 1164, 1191
Mva1269I GAATGC 1 cut(s) 1114
MvaI CCWGG 4 cut(s) 396, 432, 1164, 1191
MvnI CGCG 2 cut(s) 869, 1214
MwoI GCNNNNNNNGC 1 cut(s) 175
NciI CCSGG 1 cut(s) 816
NcoI CCATGG 2 cut(s) 250, 276
NdeI CATATG 1 cut(s) 672
NdeII GATC 6 cut(s) 350, 445, 634, 687, 802, 1322
NheI GCTAGC 1 cut(s) 919
NlaIII CATG 7 cut(s) 35, 254, 280, 292, 760, 782, 1306
NlaIV GGNNCC 1 cut(s) 610
NmeAIII GCCGAG 2 cut(s) 338, 1135
NruI TCGCGA 1 cut(s) 1214
NsiI ATGCAT 1 cut(s) 526
PceI AGGCCT 1 cut(s) 810
PciSI GCTCTTC 2 cut(s) 168, 621
PcsI WCGNNNNNNNCGW 1 cut(s) 1229
PctI GAATGC 1 cut(s) 1114
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 3 cut(s) 79, 945, 1370
PflMI CCANNNNNTGG 1 cut(s) 1234
PfoI TCCNGGA 1 cut(s) 394
PkrI GCNGC 1 cut(s) 868
PleI GAGTC 7 cut(s) 20, 35, 100, 220, 364, 704, 885
PpsI GAGTC 7 cut(s) 20, 35, 100, 220, 364, 704, 885
Psp124BI GAGCTC 1 cut(s) 550
Psp1406I AACGTT 1 cut(s) 1378
Psp6I CCWGG 4 cut(s) 394, 430, 1162, 1189
PspGI CCWGG 4 cut(s) 394, 430, 1162, 1189
PspN4I GGNNCC 1 cut(s) 610
PspPI GGNCC 2 cut(s) 1225, 1249
PsrI GAACNNNNNNTAC 2 cut(s) 459, 491
PsuI RGATCY 4 cut(s) 445, 687, 802, 1322
PvuII CAGCTG 1 cut(s) 347
RruI TCGCGA 1 cut(s) 1214
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
RseI CAYNNNNRTG 1 cut(s) 1182
SacI GAGCTC 1 cut(s) 550
SapI GCTCTTC 2 cut(s) 168, 621
SaqAI TTAA 5 cut(s) 99, 570, 1076, 1152, 1257
SatI GCNGC 1 cut(s) 867
Sau3AI GATC 6 cut(s) 350, 445, 634, 687, 802, 1322
Sau96I GGNCC 2 cut(s) 1225, 1249
SchI GAGTC 7 cut(s) 20, 35, 101, 221, 364, 704, 885
ScrFI CCNGG 5 cut(s) 396, 432, 816, 1164, 1191
SduI GDGCHC 1 cut(s) 550
SexAI ACCWGGT 1 cut(s) 1189
SfaNI GCATC 5 cut(s) 187, 262, 391, 511, 523
SinI GGWCC 2 cut(s) 1225, 1249
SmiMI CAYNNNNRTG 1 cut(s) 1182
SmlI CTYRAG 1 cut(s) 1075
SmoI CTYRAG 1 cut(s) 1075
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 3 cut(s) 227, 317, 925
SseBI AGGCCT 1 cut(s) 810
SsiI CCGC 2 cut(s) 867, 1025
SspMI CTAG 4 cut(s) 105, 734, 806, 920
SstI GAGCTC 1 cut(s) 550
StuI AGGCCT 1 cut(s) 810
StyD4I CCNGG 5 cut(s) 394, 430, 814, 1162, 1189
StyI CCWWGG 3 cut(s) 55, 250, 276
TaaI ACNGT 2 cut(s) 703, 1253
TaiI ACGT 1 cut(s) 1381
TaqI TCGA 3 cut(s) 321, 708, 881
TasI AATT 3 cut(s) 227, 317, 925
TauI GCSGC 1 cut(s) 869
TfiI GAWTC 3 cut(s) 79, 945, 1370
Tru1I TTAA 5 cut(s) 99, 570, 1076, 1152, 1257
Tru9I TTAA 5 cut(s) 99, 570, 1076, 1152, 1257
TspDTI ATGAA 3 cut(s) 277, 954, 1200
Van91I CCANNNNNTGG 1 cut(s) 1234
Vha464I CTTAAG 1 cut(s) 1075
VpaK11BI GGWCC 2 cut(s) 1225, 1249
XapI RAATTY 1 cut(s) 317
XbaI TCTAGA 1 cut(s) 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 4 cut(s) 105, 734, 806, 920
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.