Rorug06G0378100

1-aminocyclopropane-1-carboxylate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000825
Physical Location & Seq
Forward (+)
52446766 .. 52450936
4171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0378100.1

Sequence Viewer

Length: 2247 bp
ATGCTTCAAGTTGGGTATGGTGGGGGAGAAGCTGAGATGGATAAGGGACGTTCTGTTGTCGTGGGAGTGAAGCTGGACTCCAAGAGCAGAGAGCTGCTGACGTGGGCTCTCGTCAAGGTGGCTGATCCCGGTGACTCTGTTATTGCTCTTCACGTTCTTGGTAAAAATGAAATTGTGGATCGAGATGGGAAGTCGTCGCTTCTATCACTCGTCAAAGCTTTTGACTCTGTTCTTGCTGTGTATGAAGGCTTCTGTAATTTGAAGCAGGTGGATCTCAAGCTTAAAATTTGCAGAGGAACATCAATAAAGAAGATTCTAGTCCGGGAAGCGAAATCTTATAATGCATGCAAATTTATAGTGGGAACTGCTCAGAGCCACCATAAAATCCGGTCATCCACCACCGTGGCCAAGTACTGTGCTAAGAAACTGTCAAAGGATTGTGGGATACTTGCTGTAAATAACGGGAAAGTTGTGTTTAACAGGGAGGGCTCTCAATCAAGTTGTGACTCTCAAGGAAGTGGAGAGCATCGCCAAAATGGTATGCTCAGTGCATTTCACAGATCACTGCATAAGTCTTCGAAAGTACTAAATGAAGGCAGTGACAATCTGGCCTCAAAGAAAGCAAATGATCAGGTCAATTGTAAGAAAATGGAGCAGACATTAGCTAAAATCTTTTTCGAATGTACAGACACGGTTGAGAAACAGAAATGCTCGGTTTGTTCACGGCCCTCTGTGGATAATTCTAGTCACCAATCTGCTGAAGACTCTTCTGCTGAGGATGGTGAAGATAAATCCATGGCTATAGTGCCTGTACAAAGAGAAGAGGCAGAAACAGCAGGTTCAATTACTATGTTGATCAAAGAATTACCTGAAGCAAGACCTGGTTGGCCACTGCTTCGTCGTGCTGTGTTACCAGACCCTTCGGAGAGGTCTATGGTTCGGAAGATCTCGGTGGTTCAGTGGGCAATGCAATTGCCTAGTCGGCGTCATAGACAAAATAGTTGTGATCCAGGGGAAGATCAACCTTCTAGTTTGGATGGAGAAACTGGTGCAATTGTTCCGGTGGATAGTGAGGCAATGACTGCCCCATCTTCACCTGATCACTATTCAAAAGGCTTGCCTAGAGAATTGGAGGGTCTGCATGAGAAGTACTCATCTACTTGCAGATTGTTCAATTACCAGGAACTTCAGTCAGCAACATCATATTTCTTGGCTGAAAATCTGATTGGGAGAGGCGGTAGTAGTCAAGTTTATAAAGGCTGCCTTCCTGATGCCAAGGAACTTGCTGTTAAAATCTTAAAACCATCTGAAGATGTATTGAAGGAGTTTGTTCTGGAAATAGAGATTATCACTACCTTAAATCACAAGAACATTATTTCCCTTCTGGGGTTCTGTTTTGAGGATAACAATCTTCTCTTGGTTTATGATTTCTTATCAAGAGGAAGCCTCGAGGAGAATCTCCATGGAAGTAAGAAAGATCCAAATGCATTTGGTTGGAATGAGAGATATAAGGTAGCTGTGGGCGTAGCTGAGGCCTTGGATTATCTGCACAGTGGCAGTGCTCAACCAGTAATCCACAGGGATGTTAAATCATCCAATATACTGTTATCTGATGATTTTGAGCCACAGCTCTCTGATTTTGGACTGGCTAAGTGGGCACCAACCTCCTCATCACATATAACATGCACTGATGTTGCAGGCACATTTGGATACTTGGCTCCTGAGTACTTTATGTATGGCAAAGTAAACGAGAAGATTGACGTGTATGCATTTGGTGTTGTACTCCTTGAACTTCTTTCAGGAAGAAAACCTATAAGCAGTGATTATCCGAAAGGCCATGAGAGCCTAGTCATGTGGGCTAAGCCAATTTTAAATGGTGGGAAGGTGGCCCAACTATTAGATCCGAGCTTGGGAAAGTACGACCAAGTCCAAGTGGAGAGAATGGTTTTAGCAGCTACTCTTTGTATCAGACATGCCCCAAGAGCAAGGCCCCAAATGAGTTCTGTTGTGAAGCTCCTTCAAGGTGATGCAGAAATGATTAAGTGGGCAAGGCTACAAGTCCATGCTTGGGAGGAATCTGATCTCCATGACGATGAGCCCTGTCCACGTTCGAATCTGCAGTCTCACCTTAACCTTGCACTGCTTGATGTGGAGGATGACTCGCTCTCCATGAGCAGCATTGAGCAAACTGTCTCCTTAGAGGACTACCTCAAAGGCCGGTGGAGCCGCTCATCAAGCTTTGACTAA

Protein Analysis

748

Amino Acids

82.51

Weight (kDa)

6.1

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 406 - 611 3.9e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 407 - 672 2e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 339, 1254
AarI CACCTGC 1 cut(s) 256
AbsI CCTCGAGG 1 cut(s) 1448
Acc36I ACCTGC 2 cut(s) 256, 827
AccB1I GGYRCC 1 cut(s) 1657
AccBSI CCGCTC 1 cut(s) 2229
AciI CCGC 2 cut(s) 1236, 2227
AclWI GGATC 6 cut(s) 119, 186, 279, 1001, 1472, 1895
AcoI YGGCCR 2 cut(s) 405, 887
AcsI RAATTY 2 cut(s) 285, 350
AcuI CTGAAG 4 cut(s) 780, 891, 1172, 1329
AcyI GRCGYC 1 cut(s) 985
AfaI GTAC 8 cut(s) 413, 585, 685, 813, 1151, 1727, 1782, 1919
AfiI CCNNNNNNNGG 3 cut(s) 1386, 1910, 2068
AflIII ACRYGT 1 cut(s) 1761
AgsI TTSAA 8 cut(s) 8, 262, 843, 1110, 1174, 1321, 1790, 2021
AjiI CACGTC 2 cut(s) 102, 1762
AjnI CCWGG 3 cut(s) 880, 1009, 1179
AjuI GAANNNNNNNTTGG 2 cut(s) 1209, 1241
AleI CACNNNNGTG 1 cut(s) 401
AloI GAACNNNNNNTCC 2 cut(s) 1361, 1393
Alw21I GWGCWC 1 cut(s) 1564
Alw26I GTCTC 2 cut(s) 2127, 2197
AlwI GGATC 6 cut(s) 119, 186, 279, 1001, 1472, 1895
Ama87I CYCGRG 1 cut(s) 1448
AoxI GGCC 9 cut(s) 405, 609, 725, 887, 1533, 1834, 1887, 1988, 2215
ApeKI GCWGC 4 cut(s) 94, 1260, 1952, 2175
ApoI RAATTY 2 cut(s) 285, 350
ArsI GACNNNNNNTTYG 2 cut(s) 2105, 2137
AspS9I GGNCC 3 cut(s) 726, 1888, 1989
AsuC2I CCSGG 2 cut(s) 129, 323
AsuHPI GGTGA 6 cut(s) 143, 740, 794, 1086, 2036, 2117
AsuII TTCGAA 3 cut(s) 578, 678, 2111
AvaI CYCGRG 1 cut(s) 1448
BaeGI GKGCMC 1 cut(s) 1660
BalI TGGCCA 2 cut(s) 407, 889
BanI GGYRCC 1 cut(s) 1657
BanII GRGCYC 3 cut(s) 109, 491, 2100
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
BbsI GAAGAC 2 cut(s) 567, 768
Bbv12I GWGCWC 1 cut(s) 1564
BbvCI CCTCAGC 2 cut(s) 774, 1530
BbvI GCAGC 4 cut(s) 81, 1247, 1964, 2187
BccI CCATC 6 cut(s) 31, 179, 773, 1031, 1096, 1312
BceAI ACGGC 1 cut(s) 740
BciT130I CCWGG 3 cut(s) 882, 1011, 1181
BciVI GTATCC 2 cut(s) 438, 1703
BclI TGATCA 3 cut(s) 628, 855, 1099
BcnI CCSGG 2 cut(s) 129, 323
BcoDI GTCTC 2 cut(s) 2127, 2197
BfaI CTAG 6 cut(s) 317, 744, 978, 1029, 1122, 1847
BfmI CTRYAG 2 cut(s) 801, 2117
BfuAI ACCTGC 2 cut(s) 256, 827
BfuI GTATCC 2 cut(s) 438, 1703
BglI GCCNNNNNGGC 1 cut(s) 982
BglII AGATCT 1 cut(s) 945
BisI GCNGC 5 cut(s) 95, 1261, 1953, 2176, 2227
BlpI GCTNAGC 1 cut(s) 1860
BlsI GCNGC 5 cut(s) 96, 1262, 1954, 2177, 2228
BmcAI AGTACT 4 cut(s) 413, 585, 1151, 1727
Bme1390I CCNGG 5 cut(s) 129, 323, 882, 1011, 1181
BmeT110I CYCGRG 1 cut(s) 1448
BmgBI CACGTC 2 cut(s) 102, 1762
BmgT120I GGNCC 3 cut(s) 726, 1888, 1989
BmiI GGNNCC 4 cut(s) 1659, 1719, 1991, 2225
BmrFI CCNGG 5 cut(s) 129, 323, 882, 1011, 1181
BmsI GCATC 3 cut(s) 535, 1261, 2017
BpiI GAAGAC 2 cut(s) 567, 768
BplI GAGNNNNNCTC 6 cut(s) 1136, 1168, 1431, 1463, 2144, 2176
Bpu10I CCTNAGC 2 cut(s) 774, 1530
Bpu1102I GCTNAGC 1 cut(s) 1860
Bpu14I TTCGAA 3 cut(s) 578, 678, 2111
BpuEI CTTGAG 2 cut(s) 260, 495
BpuMI CCSGG 2 cut(s) 129, 323
BsaBI GATNNNNATC 1 cut(s) 1407
BsaHI GRCGYC 1 cut(s) 985
BsaJI CCNNGG 6 cut(s) 402, 795, 1010, 1275, 1462, 1536
BsaWI WCCGGW 2 cut(s) 387, 1060
BsaXI ACNNNNNCTCC 4 cut(s) 1222, 1252, 2150, 2180
Bsc4I CCNNNNNNNGG 3 cut(s) 1386, 1910, 2068
Bse118I RCCGGY 1 cut(s) 2217
Bse1I ACTGG 3 cut(s) 1051, 1568, 1650
Bse3DI GCAATG 2 cut(s) 972, 1083
Bse8I GATNNNNATC 1 cut(s) 1407
BseBI CCWGG 3 cut(s) 882, 1011, 1181
BseDI CCNNGG 6 cut(s) 402, 795, 1010, 1275, 1462, 1536
BseGI GGATG 6 cut(s) 392, 784, 1042, 1588, 1592, 2161
BseJI GATNNNNATC 1 cut(s) 1407
BseLI CCNNNNNNNGG 3 cut(s) 1386, 1910, 2068
BseMI GCAATG 2 cut(s) 972, 1083
BseMII CTCAG 6 cut(s) 24, 383, 559, 765, 1521, 1713
BseNI ACTGG 3 cut(s) 1051, 1568, 1650
BseRI GAGGAG 2 cut(s) 1466, 1657
BseSI GKGCMC 1 cut(s) 1660
BseXI GCAGC 4 cut(s) 81, 1247, 1964, 2187
BsgI GTGCAG 1 cut(s) 1532
BshFI GGCC 9 cut(s) 407, 611, 727, 889, 1535, 1836, 1889, 1990, 2217
BshNI GGYRCC 1 cut(s) 1657
BsiHKAI GWGCWC 1 cut(s) 1564
BsiHKCI CYCGRG 1 cut(s) 1448
BsiSI CCGG 5 cut(s) 129, 322, 388, 1061, 2218
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 3 cut(s) 1386, 1910, 2068
BsmAI GTCTC 2 cut(s) 2127, 2197
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 9 cut(s) 407, 611, 727, 889, 1535, 1836, 1889, 1990, 2217
BsoBI CYCGRG 1 cut(s) 1448
Bsp119I TTCGAA 3 cut(s) 578, 678, 2111
Bsp1286I GDGCHC 5 cut(s) 109, 491, 1564, 1660, 2100
Bsp1407I TGTACA 2 cut(s) 683, 811
Bsp1720I GCTNAGC 1 cut(s) 1860
Bsp19I CCATGG 2 cut(s) 795, 1462
BspACI CCGC 2 cut(s) 1236, 2227
BspANI GGCC 9 cut(s) 407, 611, 727, 889, 1535, 1836, 1889, 1990, 2217
BspCNI CTCAG 6 cut(s) 25, 382, 558, 766, 1522, 1714
BspLI GGNNCC 4 cut(s) 1659, 1719, 1991, 2225
BspMAI CTGCAG 1 cut(s) 2121
BspMI ACCTGC 2 cut(s) 256, 827
BspPI GGATC 6 cut(s) 119, 186, 279, 1001, 1472, 1895
BspQI GCTCTTC 1 cut(s) 153
BspT104I TTCGAA 3 cut(s) 578, 678, 2111
BspT107I GGYRCC 1 cut(s) 1657
BsrBI CCGCTC 1 cut(s) 2229
BsrDI GCAATG 2 cut(s) 972, 1083
BsrFI RCCGGY 1 cut(s) 2217
BsrGI TGTACA 2 cut(s) 683, 811
BsrI ACTGG 3 cut(s) 1051, 1568, 1650
BssAI RCCGGY 1 cut(s) 2217
BssECI CCNNGG 6 cut(s) 402, 795, 1010, 1275, 1462, 1536
BssNI GRCGYC 1 cut(s) 985
BssT1I CCWWGG 4 cut(s) 795, 1275, 1462, 1536
Bst2UI CCWGG 3 cut(s) 882, 1011, 1181
Bst4CI ACNGT 7 cut(s) 403, 416, 429, 694, 1553, 1605, 2191
Bst6I CTCTTC 3 cut(s) 153, 772, 816
BstACI GRCGYC 1 cut(s) 985
BstAPI GCANNNNNTGC 1 cut(s) 1082
BstAUI TGTACA 2 cut(s) 683, 811
BstBI TTCGAA 3 cut(s) 578, 678, 2111
BstC8I GCNNGC 3 cut(s) 346, 1118, 1699
BstDSI CCRYGG 3 cut(s) 402, 795, 1462
BstF5I GGATG 6 cut(s) 392, 784, 1042, 1588, 1592, 2161
BstMAI GTCTC 2 cut(s) 2127, 2197
BstMWI GCNNNNNNNGC 8 cut(s) 833, 982, 1082, 1655, 1842, 1982, 2223, 2235
BstNI CCWGG 3 cut(s) 882, 1011, 1181
BstNSI RCATGY 3 cut(s) 348, 1686, 1976
BstSCI CCNGG 5 cut(s) 127, 321, 880, 1009, 1179
BstSFI CTRYAG 2 cut(s) 801, 2117
BstSLI GKGCMC 1 cut(s) 1660
BstV1I GCAGC 4 cut(s) 81, 1247, 1964, 2187
BstV2I GAAGAC 2 cut(s) 567, 768
BstX2I RGATCY 4 cut(s) 271, 945, 1477, 1900
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 4 cut(s) 271, 945, 1477, 1900
BsuI GTATCC 2 cut(s) 438, 1703
BsuRI GGCC 9 cut(s) 407, 611, 727, 889, 1535, 1836, 1889, 1990, 2217
BtgI CCRYGG 3 cut(s) 402, 795, 1462
BtgZI GCGATG 1 cut(s) 512
BtrI CACGTC 2 cut(s) 102, 1762
BtsCI GGATG 6 cut(s) 392, 784, 1042, 1588, 1592, 2161
BtsI GCAGTG 6 cut(s) 563, 604, 890, 1564, 1825, 2138
BveI ACCTGC 2 cut(s) 256, 827
Cac8I GCNNGC 3 cut(s) 346, 1118, 1699
Cfr10I RCCGGY 1 cut(s) 2217
Cfr13I GGNCC 3 cut(s) 726, 1888, 1989
CseI GACGC 1 cut(s) 974
CsiI ACCWGGT 1 cut(s) 880
Csp6I GTAC 8 cut(s) 412, 584, 684, 812, 1150, 1726, 1781, 1918
CviQI GTAC 8 cut(s) 412, 584, 684, 812, 1150, 1726, 1781, 1918
DraI TTTAAA 1 cut(s) 1872
EaeI YGGCCR 2 cut(s) 405, 887
Eam1104I CTCTTC 3 cut(s) 153, 772, 816
EarI CTCTTC 3 cut(s) 153, 772, 816
Eco130I CCWWGG 4 cut(s) 795, 1275, 1462, 1536
Eco147I AGGCCT 1 cut(s) 1535
Eco24I GRGCYC 3 cut(s) 109, 491, 2100
Eco57I CTGAAG 4 cut(s) 780, 891, 1172, 1329
Eco88I CYCGRG 1 cut(s) 1448
EcoO109I RGGNCCY 1 cut(s) 1989
EcoRII CCWGG 3 cut(s) 880, 1009, 1179
EcoT14I CCWWGG 4 cut(s) 795, 1275, 1462, 1536
EcoT22I ATGCAT 3 cut(s) 346, 1489, 1771
EcoT38I GRGCYC 3 cut(s) 109, 491, 2100
ErhI CCWWGG 4 cut(s) 795, 1275, 1462, 1536
FalI AAGNNNNNCTT 2 cut(s) 1248, 1280
FaqI GGGAC 1 cut(s) 60
FbaI TGATCA 3 cut(s) 628, 855, 1099
Fnu4HI GCNGC 5 cut(s) 95, 1261, 1953, 2176, 2227
FokI GGATG 6 cut(s) 379, 791, 1049, 1579, 1595, 2168
FriOI GRGCYC 3 cut(s) 109, 491, 2100
Fsp4HI GCNGC 5 cut(s) 95, 1261, 1953, 2176, 2227
FspBI CTAG 6 cut(s) 317, 744, 978, 1029, 1122, 1847
GluI GCNGC 5 cut(s) 95, 1261, 1953, 2176, 2227
HaeIII GGCC 9 cut(s) 407, 611, 727, 889, 1535, 1836, 1889, 1990, 2217
HapII CCGG 5 cut(s) 129, 322, 388, 1061, 2218
HgaI GACGC 1 cut(s) 974
Hin1I GRCGYC 1 cut(s) 985
HindIII AAGCTT 3 cut(s) 216, 278, 2236
HpaII CCGG 5 cut(s) 129, 322, 388, 1061, 2218
HphI GGTGA 6 cut(s) 143, 740, 794, 1086, 2036, 2117
Hpy166II GTNNAC 3 cut(s) 722, 1747, 2105
Hpy188III TCNNGA 6 cut(s) 182, 1268, 1334, 1437, 1721, 1800
Hpy8I GTNNAC 3 cut(s) 722, 1747, 2105
Hpy99I CGWCG 2 cut(s) 199, 903
HpyAV CCTTC 9 cut(s) 239, 587, 930, 1035, 1274, 1315, 1391, 1876, 2027
HpyCH4III ACNGT 7 cut(s) 403, 416, 429, 694, 1553, 1605, 2191
HpyCH4IV ACGT 5 cut(s) 49, 101, 153, 1761, 2107
HpyF10VI GCNNNNNNNGC 8 cut(s) 833, 982, 1082, 1655, 1842, 1982, 2223, 2235
HpySE526I ACGT 5 cut(s) 49, 101, 153, 1761, 2107
Hsp92I GRCGYC 1 cut(s) 985
Ksp22I TGATCA 3 cut(s) 628, 855, 1099
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 4 cut(s) 652, 1723, 2019, 2223
Lsp1109I GCAGC 4 cut(s) 81, 1247, 1964, 2187
LweI GCATC 3 cut(s) 535, 1261, 2017
MabI ACCWGGT 1 cut(s) 880
MaeI CTAG 6 cut(s) 317, 744, 978, 1029, 1122, 1847
MaeII ACGT 5 cut(s) 49, 101, 153, 1761, 2107
MaeIII GTNAC 5 cut(s) 131, 503, 599, 746, 909
MbiI CCGCTC 1 cut(s) 2229
MfeI CAATTG 3 cut(s) 637, 971, 1053
MflI RGATCY 4 cut(s) 271, 945, 1477, 1900
MhlI GDGCHC 5 cut(s) 109, 491, 1564, 1660, 2100
MlsI TGGCCA 2 cut(s) 407, 889
MluNI TGGCCA 2 cut(s) 407, 889
MlyI GAGTC 6 cut(s) 71, 128, 218, 500, 758, 2153
MmeI TCCRAC 1 cut(s) 1475
Mox20I TGGCCA 2 cut(s) 407, 889
Mph1103I ATGCAT 3 cut(s) 346, 1489, 1771
MscI TGGCCA 2 cut(s) 407, 889
MseI TTAA 9 cut(s) 282, 477, 1290, 1298, 1358, 1587, 1871, 2040, 2130
MslI CAYNNNNRTG 3 cut(s) 401, 1581, 2091
Msp20I TGGCCA 2 cut(s) 407, 889
MspI CCGG 5 cut(s) 129, 322, 388, 1061, 2218
MspR9I CCNGG 5 cut(s) 129, 323, 882, 1011, 1181
MunI CAATTG 3 cut(s) 637, 971, 1053
MvaI CCWGG 3 cut(s) 882, 1011, 1181
MwoI GCNNNNNNNGC 8 cut(s) 833, 982, 1082, 1655, 1842, 1982, 2223, 2235
NciI CCSGG 2 cut(s) 129, 323
NcoI CCATGG 2 cut(s) 795, 1462
NlaIV GGNNCC 4 cut(s) 1659, 1719, 1991, 2225
NmuCI GTSAC 4 cut(s) 131, 503, 599, 746
NsiI ATGCAT 3 cut(s) 346, 1489, 1771
NspI RCATGY 3 cut(s) 348, 1686, 1976
NspV TTCGAA 3 cut(s) 578, 678, 2111
OliI CACNNNNGTG 1 cut(s) 401
PaeI GCATGC 1 cut(s) 348
PaeR7I CTCGAG 1 cut(s) 1448
PaqCI CACCTGC 1 cut(s) 256
PceI AGGCCT 1 cut(s) 1535
PciSI GCTCTTC 1 cut(s) 153
PfeI GAWTC 4 cut(s) 313, 1456, 2075, 2113
PflFI GACNNNGTC 1 cut(s) 1925
PfoI TCCNGGA 1 cut(s) 321
PkrI GCNGC 5 cut(s) 96, 1262, 1954, 2177, 2228
PleI GAGTC 6 cut(s) 71, 128, 218, 500, 758, 2153
PpsI GAGTC 6 cut(s) 71, 128, 218, 500, 758, 2153
PsiI TTATAA 2 cut(s) 339, 1254
Psp6I CCWGG 3 cut(s) 880, 1009, 1179
PspGI CCWGG 3 cut(s) 880, 1009, 1179
PspN4I GGNNCC 4 cut(s) 1659, 1719, 1991, 2225
PspPI GGNCC 3 cut(s) 726, 1888, 1989
PspXI VCTCGAGB 1 cut(s) 1448
PstI CTGCAG 1 cut(s) 2121
PsuI RGATCY 4 cut(s) 271, 945, 1477, 1900
PsyI GACNNNGTC 1 cut(s) 1925
RsaI GTAC 8 cut(s) 413, 585, 685, 813, 1151, 1727, 1782, 1919
RsaNI GTAC 8 cut(s) 412, 584, 684, 812, 1150, 1726, 1781, 1918
RseI CAYNNNNRTG 3 cut(s) 401, 1581, 2091
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 9 cut(s) 282, 477, 1290, 1298, 1358, 1587, 1871, 2040, 2130
SatI GCNGC 5 cut(s) 95, 1261, 1953, 2176, 2227
Sau96I GGNCC 3 cut(s) 726, 1888, 1989
ScaI AGTACT 4 cut(s) 413, 585, 1151, 1727
SchI GAGTC 6 cut(s) 71, 128, 218, 500, 758, 2153
ScrFI CCNGG 5 cut(s) 129, 323, 882, 1011, 1181
SduI GDGCHC 5 cut(s) 109, 491, 1564, 1660, 2100
SexAI ACCWGGT 1 cut(s) 880
SfaNI GCATC 3 cut(s) 535, 1261, 2017
SfcI CTRYAG 2 cut(s) 801, 2117
Sfr274I CTCGAG 1 cut(s) 1448
SfuI TTCGAA 3 cut(s) 578, 678, 2111
SlaI CTCGAG 1 cut(s) 1448
SmiMI CAYNNNNRTG 3 cut(s) 401, 1581, 2091
SmlI CTYRAG 3 cut(s) 275, 510, 1448
SmoI CTYRAG 3 cut(s) 275, 510, 1448
SphI GCATGC 1 cut(s) 348
SseBI AGGCCT 1 cut(s) 1535
SsiI CCGC 2 cut(s) 1236, 2227
SspMI CTAG 6 cut(s) 317, 744, 978, 1029, 1122, 1847
StuI AGGCCT 1 cut(s) 1535
StyD4I CCNGG 5 cut(s) 127, 321, 880, 1009, 1179
StyI CCWWGG 4 cut(s) 795, 1275, 1462, 1536
TaaI ACNGT 7 cut(s) 403, 416, 429, 694, 1553, 1605, 2191
TaiI ACGT 5 cut(s) 52, 104, 156, 1764, 2110
TaqI TCGA 5 cut(s) 181, 578, 678, 1449, 2111
TatI WGTACW 7 cut(s) 411, 583, 683, 811, 1149, 1725, 1780
TauI GCSGC 1 cut(s) 2229
TfiI GAWTC 4 cut(s) 313, 1456, 2075, 2113
Tru1I TTAA 9 cut(s) 282, 477, 1290, 1298, 1358, 1587, 1871, 2040, 2130
Tru9I TTAA 9 cut(s) 282, 477, 1290, 1298, 1358, 1587, 1871, 2040, 2130
TseFI GTSAC 4 cut(s) 131, 503, 599, 746
TseI GCWGC 4 cut(s) 94, 1260, 1952, 2175
Tsp45I GTSAC 4 cut(s) 131, 503, 599, 746
TspDTI ATGAA 3 cut(s) 183, 258, 606
Tth111I GACNNNGTC 1 cut(s) 1925
XapI RAATTY 2 cut(s) 285, 350
XceI RCATGY 3 cut(s) 348, 1686, 1976
XhoI CTCGAG 1 cut(s) 1448
XspI CTAG 6 cut(s) 317, 744, 978, 1029, 1122, 1847
ZrmI AGTACT 4 cut(s) 413, 585, 1151, 1727
Zsp2I ATGCAT 3 cut(s) 346, 1489, 1771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.