Rw6G003300

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000825
Physical Location & Seq
Forward (+)
5251661 .. 5255167
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003300.1

Sequence Viewer

Length: 786 bp
ATGTCCAGGAGGCCAGTAAATCCTTCTAGGCGCATTGGTGATGGTGGAAGCATCCCATTTGTGGGTGCCGTGCAGGCCAAAGCACGCTCATCACCTGTACTGTCTGTGGCGCTTGTGCTTGTGGGTACAGTTCTTCTTGTCTGCTATGCTTTTAGCGGGTCAGGTGGAGTGAGCAGTAAAGAGGCTGTGATTAAACTTGAAGGTGGTGTTTCTTGTACATATGAAGTTCAGAGAGCAATACCTATACTAAAGAAGGCATATGGTGATAGTATGCATAAGGTATTGCATGTAGGCCCTGAAACGTGTTCAGTGGTATCTAAATTATTAAAAGAGGAGGATACTGAAGCCTGGGGTGTGGAACCATATGACTTGGAAGATGTTGATGCAAATTGCAAGAGTCTTGTGAGCAAAGGCATTGTGCGTGCTGCTGATATAAAGTTTCCTCTGCCATACCGGGCAAAATCATTTTCTCTGGTAATAGTATCAGATGCATTAGATTACTTGTCTCCGAAGTACCTCAACAGAACTCTTCCAGAGTTAGCAAGGGTATCTGCTGATGGCGTTATTATATTCTCTGGTTATCCAGGTCAACAAAGAGCTAAAGTTGCAGAGCTATCCAAATTTGGTCGTCCGGCCAAATTGCGAAGCTCGTCCTGGTGGATAAGATATTTTGTTCAAACAAGCTTAGAAGAGAATGAAGCAGCCTCGAAGAAGTTTGAACAGGCTTCATTAAAGAGATCTTATAAGCCTGAATGTCAAGTTTTCCACCTAAAGTCATACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

261

Amino Acids

28.62

Weight (kDa)

9.32

Isoelectric Point (pI)

48.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AccB1I GGYRCC 1 cut(s) 65
AciI CCGC 1 cut(s) 156
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 363
AfaI GTAC 4 cut(s) 99, 127, 217, 515
AfiI CCNNNNNNNGG 2 cut(s) 61, 62
AflIII ACRYGT 1 cut(s) 302
AgsI TTSAA 3 cut(s) 200, 677, 719
AjnI CCWGG 4 cut(s) 5, 347, 583, 653
AluBI AGCT 4 cut(s) 599, 613, 648, 684
AluI AGCT 4 cut(s) 599, 613, 648, 684
Alw26I GTCTC 1 cut(s) 510
AoxI GGCC 4 cut(s) 11, 75, 292, 633
ApeKI GCWGC 2 cut(s) 425, 701
ApoI RAATTY 1 cut(s) 620
AspLEI GCGC 2 cut(s) 33, 112
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 455
AsuHPI GGTGA 3 cut(s) 50, 84, 275
BanI GGYRCC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 412, 713
BccI CCATC 2 cut(s) 35, 551
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 4 cut(s) 7, 349, 585, 655
BciVI GTATCC 1 cut(s) 331
BcnI CCSGG 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 1 cut(s) 27
BfoI RGCGCY 1 cut(s) 113
BfuI GTATCC 1 cut(s) 331
BglI GCCNNNNNGGC 1 cut(s) 74
BglII AGATCT 1 cut(s) 737
BisI GCNGC 2 cut(s) 426, 702
BlsI GCNGC 2 cut(s) 427, 703
Bme1390I CCNGG 5 cut(s) 7, 349, 455, 585, 655
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 67, 360
BmrFI CCNGG 5 cut(s) 7, 349, 455, 585, 655
BmsI GCATC 3 cut(s) 60, 373, 478
BpuMI CCSGG 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 62
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 4 cut(s) 7, 349, 585, 655
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 1 cut(s) 51
BseLI CCNNNNNNNGG 2 cut(s) 61, 62
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 412, 713
BsgI GTGCAG 1 cut(s) 92
BshFI GGCC 4 cut(s) 13, 77, 294, 635
BshNI GGYRCC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 454, 632
BslI CCNNNNNNNGG 2 cut(s) 61, 62
BsmAI GTCTC 1 cut(s) 510
BsnI GGCC 4 cut(s) 13, 77, 294, 635
Bsp1407I TGTACA 1 cut(s) 215
Bsp143I GATC 1 cut(s) 737
BspACI CCGC 1 cut(s) 156
BspANI GGCC 4 cut(s) 13, 77, 294, 635
BspLI GGNNCC 2 cut(s) 67, 360
BspT107I GGYRCC 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 215
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 1 cut(s) 737
Bst2UI CCWGG 4 cut(s) 7, 349, 585, 655
Bst4CI ACNGT 2 cut(s) 102, 130
Bst6I CTCTTC 2 cut(s) 534, 684
BstAUI TGTACA 1 cut(s) 215
BstC8I GCNNGC 3 cut(s) 75, 85, 423
BstDEI CTNAG 1 cut(s) 685
BstF5I GGATG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 2 cut(s) 33, 112
BstKTI GATC 1 cut(s) 740
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 1 cut(s) 737
BstMWI GCNNNNNNNGC 2 cut(s) 74, 605
BstNI CCWGG 4 cut(s) 7, 349, 585, 655
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 5 cut(s) 5, 347, 453, 583, 653
BstV1I GCAGC 2 cut(s) 412, 713
BstX2I RGATCY 1 cut(s) 737
BstYI RGATCY 1 cut(s) 737
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 4 cut(s) 13, 77, 294, 635
BtsCI GGATG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 3 cut(s) 75, 85, 423
CfoI GCGC 2 cut(s) 33, 112
Cfr13I GGNCC 1 cut(s) 293
Csp6I GTAC 4 cut(s) 98, 126, 216, 514
CviAII CATG 1 cut(s) 287
CviQI GTAC 4 cut(s) 98, 126, 216, 514
DdeI CTNAG 1 cut(s) 685
DpnI GATC 1 cut(s) 739
DpnII GATC 1 cut(s) 737
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 2 cut(s) 534, 684
EarI CTCTTC 2 cut(s) 534, 684
Eco57I CTGAAG 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 4 cut(s) 5, 347, 583, 653
EcoT22I ATGCAT 2 cut(s) 276, 493
FaeI CATG 1 cut(s) 290
FatI CATG 1 cut(s) 286
FauI CCCGC 1 cut(s) 149
FauNDI CATATG 3 cut(s) 220, 259, 364
Fnu4HI GCNGC 2 cut(s) 426, 702
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 2 cut(s) 426, 702
FspBI CTAG 1 cut(s) 27
GlaI GCGC 2 cut(s) 32, 111
GluI GCNGC 2 cut(s) 426, 702
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 4 cut(s) 13, 77, 294, 635
HapII CCGG 2 cut(s) 454, 632
HhaI GCGC 2 cut(s) 33, 112
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 2 cut(s) 31, 110
HinP1I GCGC 2 cut(s) 31, 110
HincII GTYRAC 1 cut(s) 590
HindII GTYRAC 1 cut(s) 590
HindIII AAGCTT 1 cut(s) 682
HinfI GANTC 1 cut(s) 397
HpaII CCGG 2 cut(s) 454, 632
HphI GGTGA 3 cut(s) 50, 84, 275
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 3 cut(s) 231, 487, 510
Hpy188III TCNNGA 1 cut(s) 533
Hpy8I GTNNAC 1 cut(s) 590
HpyAV CCTTC 3 cut(s) 33, 194, 247
HpyCH4III ACNGT 2 cut(s) 102, 130
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 7 cut(s) 73, 274, 286, 386, 393, 491, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 605
HpyF3I CTNAG 1 cut(s) 685
HpySE526I ACGT 1 cut(s) 302
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 2 cut(s) 31, 110
Kzo9I GATC 1 cut(s) 737
Lsp1109I GCAGC 2 cut(s) 412, 713
LweI GCATC 3 cut(s) 60, 373, 478
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 302
MalI GATC 1 cut(s) 739
MboI GATC 1 cut(s) 737
MboII GAAGA 5 cut(s) 125, 386, 521, 701, 721
MflI RGATCY 1 cut(s) 737
MluCI AATT 4 cut(s) 320, 388, 620, 638
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 7 cut(s) 3, 175, 325, 328, 453, 527, 715
Mph1103I ATGCAT 2 cut(s) 276, 493
MseI TTAA 3 cut(s) 192, 326, 731
MspI CCGG 2 cut(s) 454, 632
MspR9I CCNGG 5 cut(s) 7, 349, 455, 585, 655
MvaI CCWGG 4 cut(s) 7, 349, 585, 655
MwoI GCNNNNNNNGC 2 cut(s) 74, 605
NciI CCSGG 1 cut(s) 455
NdeI CATATG 3 cut(s) 220, 259, 364
NdeII GATC 1 cut(s) 737
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 2 cut(s) 67, 360
NsiI ATGCAT 2 cut(s) 276, 493
NspI RCATGY 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 2 cut(s) 427, 703
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
PsiI TTATAA 1 cut(s) 744
Psp6I CCWGG 4 cut(s) 5, 347, 583, 653
PspGI CCWGG 4 cut(s) 5, 347, 583, 653
PspN4I GGNNCC 2 cut(s) 67, 360
PspPI GGNCC 1 cut(s) 293
PsuI RGATCY 1 cut(s) 737
RsaI GTAC 4 cut(s) 99, 127, 217, 515
RsaNI GTAC 4 cut(s) 98, 126, 216, 514
SaqAI TTAA 3 cut(s) 192, 326, 731
SatI GCNGC 2 cut(s) 426, 702
Sau3AI GATC 1 cut(s) 737
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 5 cut(s) 7, 349, 455, 585, 655
SfaNI GCATC 3 cut(s) 60, 373, 478
Sse9I AATT 4 cut(s) 320, 388, 620, 638
SsiI CCGC 1 cut(s) 156
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 5 cut(s) 5, 347, 453, 583, 653
TaaI ACNGT 2 cut(s) 102, 130
TaiI ACGT 1 cut(s) 305
TaqI TCGA 1 cut(s) 707
TasI AATT 4 cut(s) 320, 388, 620, 638
TatI WGTACW 2 cut(s) 97, 215
Tru1I TTAA 3 cut(s) 192, 326, 731
Tru9I TTAA 3 cut(s) 192, 326, 731
TscAI CASTG 1 cut(s) 315
TseI GCWGC 2 cut(s) 425, 701
TspDTI ATGAA 3 cut(s) 237, 711, 717
TspRI CASTG 1 cut(s) 315
XapI RAATTY 1 cut(s) 620
XceI RCATGY 1 cut(s) 290
XspI CTAG 1 cut(s) 27
Zsp2I ATGCAT 2 cut(s) 276, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.