Rh6BG501700

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000825
Physical Location & Seq
Forward (+)
70105987 .. 70109035
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG501700.1

Sequence Viewer

Length: 711 bp
ATGTCGAGGAGGCAAGCGAATGCGAACCTATCTCGGCGACTGGGAGATGCCGGAGGCGGTTGGCTCTCAGGCTCGTCCTATTCTAAATCAAGGTCTTCTCACATCTTATATGTCGCTCTCATTGCCGTGGGAGCATTGCTTATTCTTGCCTATTCACACAGTGGCACAGGTGGAATTGGTAATACTAGAGGGGCTATTAGCAGGGTTCAAGGTGATTACTCATGCACAGCGGAGGTCCAGCGAGCAATTCCTGTTTTGAAGAAAGCATATGGTGACAGCATGCATAAGGTTTTGCACGTTGGCCCTGATACTTGTACAGTGGTTTATAAATTGTGTAGTGAGGAGGAAACTGAAGCCTGGGGTGTGGATCCGTACGATGTAGAGGATGCTGATAGAAGTTGCAAAGCTCTTGTGCGCAAAGGCATAGTGCGTGTTGATGATATCAAGTATCCACTTCCATACAGGCCAAAATCATTTTCTGTTGTCATTGTTTCGGATTCTTTGGATTATTTGTCTGAAAGATACCTCAACAAGACCCTTCCGGATCTGGCTAGGGTATCAGCTGATGGTGTTGTAATCTTTACAGGCAAGATCTTATGTCAACTTGTAAAATCCATTTATCTGTGTTGCTTTCTACATGTGATCTTATATCTGTGTCTCTTATGTGTTATTAGAAAGTCTTTTGTTTGTCGAACCATATTTTCACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

236

Amino Acids

25.94

Weight (kDa)

8.97

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
Acc16I TGCGCA 1 cut(s) 416
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 2 cut(s) 57, 230
AclWI GGATC 3 cut(s) 362, 375, 552
AcuI CTGAAG 1 cut(s) 372
AdeI CACNNNGTG 1 cut(s) 161
AfaI GTAC 2 cut(s) 316, 374
AflIII ACRYGT 1 cut(s) 637
AgsI TTSAA 2 cut(s) 209, 259
AjnI CCWGG 1 cut(s) 356
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AluBI AGCT 2 cut(s) 407, 563
AluI AGCT 2 cut(s) 407, 563
Alw26I GTCTC 1 cut(s) 662
AlwI GGATC 3 cut(s) 362, 375, 552
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 2 cut(s) 301, 464
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 2 cut(s) 235, 302
AsuHPI GGTGA 2 cut(s) 224, 284
AvaII GGWCC 1 cut(s) 235
BamHI GGATCC 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 87
BccI CCATC 1 cut(s) 560
BceAI ACGGC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 358
BciVI GTATCC 1 cut(s) 459
BcoDI GTCTC 1 cut(s) 662
BfaI CTAG 2 cut(s) 186, 552
BfmI CTRYAG 1 cut(s) 707
BfuI GTATCC 1 cut(s) 459
BglII AGATCT 1 cut(s) 591
Bme1390I CCNGG 1 cut(s) 358
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 235, 302
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 1 cut(s) 358
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 2 cut(s) 37, 376
BmuI ACTGGG 1 cut(s) 50
BpiI GAAGAC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 126, 357
BsaWI WCCGGW 1 cut(s) 541
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 2 cut(s) 120, 134
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 2 cut(s) 126, 357
BseGI GGATG 1 cut(s) 391
BseMI GCAATG 2 cut(s) 120, 134
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 2 cut(s) 22, 356
BshFI GGCC 2 cut(s) 303, 466
BsiSI CCGG 2 cut(s) 51, 542
BsiWI CGTACG 1 cut(s) 372
BsmAI GTCTC 1 cut(s) 662
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 2 cut(s) 303, 466
Bsp13I TCCGGA 1 cut(s) 541
Bsp1407I TGTACA 1 cut(s) 314
Bsp143I GATC 4 cut(s) 367, 544, 591, 642
BspACI CCGC 2 cut(s) 57, 230
BspANI GGCC 2 cut(s) 303, 466
BspCNI CTCAG 1 cut(s) 80
BspEI TCCGGA 1 cut(s) 541
BspLI GGNNCC 1 cut(s) 369
BspPI GGATC 3 cut(s) 362, 375, 552
BsrDI GCAATG 2 cut(s) 120, 134
BsrGI TGTACA 1 cut(s) 314
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 126, 357
BssMI GATC 4 cut(s) 367, 544, 591, 642
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 3 cut(s) 161, 319, 708
BstAUI TGTACA 1 cut(s) 314
BstC8I GCNNGC 3 cut(s) 15, 243, 281
BstDEI CTNAG 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 391
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 4 cut(s) 370, 547, 594, 645
BstMAI GTCTC 1 cut(s) 662
BstMBI GATC 4 cut(s) 367, 544, 591, 642
BstMWI GCNNNNNNNGC 2 cut(s) 122, 131
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 2 cut(s) 283, 641
BstSCI CCNGG 1 cut(s) 356
BstSFI CTRYAG 1 cut(s) 707
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 3 cut(s) 367, 544, 591
BstYI RGATCY 3 cut(s) 367, 544, 591
BsuI GTATCC 1 cut(s) 459
BsuRI GGCC 2 cut(s) 303, 466
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 3 cut(s) 166, 324, 704
Cac8I GCNNGC 3 cut(s) 15, 243, 281
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 2 cut(s) 235, 302
Csp6I GTAC 2 cut(s) 315, 373
CviAII CATG 3 cut(s) 222, 280, 638
CviJI RGCY 9 cut(s) 64, 72, 194, 303, 356, 407, 466, 551, 563
CviKI_1 RGCY 9 cut(s) 64, 72, 194, 303, 356, 407, 466, 551, 563
CviQI GTAC 2 cut(s) 315, 373
DdeI CTNAG 1 cut(s) 67
DpnI GATC 4 cut(s) 369, 546, 593, 644
DpnII GATC 4 cut(s) 367, 544, 591, 642
DraIII CACNNNGTG 1 cut(s) 161
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 356
EcoRV GATATC 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 285
FaeI CATG 3 cut(s) 225, 283, 641
FatI CATG 3 cut(s) 221, 279, 637
FauNDI CATATG 1 cut(s) 268
FokI GGATG 1 cut(s) 398
FspBI CTAG 2 cut(s) 186, 552
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 1 cut(s) 416
HaeIII GGCC 2 cut(s) 303, 466
HapII CCGG 2 cut(s) 51, 542
HhaI GCGC 1 cut(s) 417
Hin1II CATG 3 cut(s) 225, 283, 641
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 1 cut(s) 602
HindII GTYRAC 1 cut(s) 602
HinfI GANTC 1 cut(s) 497
HpaII CCGG 2 cut(s) 51, 542
HphI GGTGA 2 cut(s) 224, 284
Hpy166II GTNNAC 1 cut(s) 602
Hpy188I TCNGA 2 cut(s) 496, 517
Hpy188III TCNNGA 1 cut(s) 542
Hpy8I GTNNAC 1 cut(s) 602
HpyAV CCTTC 1 cut(s) 548
HpyCH4III ACNGT 3 cut(s) 161, 319, 708
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 4 cut(s) 225, 283, 295, 402
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 131
HpyF3I CTNAG 1 cut(s) 67
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 3 cut(s) 225, 283, 641
HspAI GCGC 1 cut(s) 415
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 4 cut(s) 367, 544, 591, 642
LmnI GCTCC 1 cut(s) 131
LweI GCATC 2 cut(s) 37, 376
MaeI CTAG 2 cut(s) 186, 552
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 272
MalI GATC 4 cut(s) 369, 546, 593, 644
MboI GATC 4 cut(s) 367, 544, 591, 642
MboII GAAGA 2 cut(s) 87, 271
MflI RGATCY 3 cut(s) 367, 544, 591
MluCI AATT 3 cut(s) 174, 246, 329
MnlI CCTC 8 cut(s) 3, 47, 182, 226, 334, 337, 376, 536
Mph1103I ATGCAT 1 cut(s) 285
MroI TCCGGA 1 cut(s) 541
MslI CAYNNNNRTG 1 cut(s) 125
MspA1I CMGCKG 2 cut(s) 230, 563
MspI CCGG 2 cut(s) 51, 542
MspR9I CCNGG 1 cut(s) 358
Mva1269I GAATGC 1 cut(s) 25
MvaI CCWGG 1 cut(s) 358
MwoI GCNNNNNNNGC 2 cut(s) 122, 131
NdeI CATATG 1 cut(s) 268
NdeII GATC 4 cut(s) 367, 544, 591, 642
NlaIII CATG 3 cut(s) 225, 283, 641
NlaIV GGNNCC 1 cut(s) 369
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 272
NsbI TGCGCA 1 cut(s) 416
NsiI ATGCAT 1 cut(s) 285
NspI RCATGY 2 cut(s) 283, 641
PaeI GCATGC 1 cut(s) 283
PciI ACATGT 1 cut(s) 637
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 497
Pfl23II CGTACG 1 cut(s) 372
PscI ACATGT 1 cut(s) 637
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 356
PspLI CGTACG 1 cut(s) 372
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 2 cut(s) 235, 302
PsuI RGATCY 3 cut(s) 367, 544, 591
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 2 cut(s) 316, 374
RsaNI GTAC 2 cut(s) 315, 373
RseI CAYNNNNRTG 1 cut(s) 125
Sau3AI GATC 4 cut(s) 367, 544, 591, 642
Sau96I GGNCC 2 cut(s) 235, 302
ScrFI CCNGG 1 cut(s) 358
SfaNI GCATC 2 cut(s) 37, 376
SfcI CTRYAG 1 cut(s) 707
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 125
SphI GCATGC 1 cut(s) 283
Sse9I AATT 3 cut(s) 174, 246, 329
SsiI CCGC 2 cut(s) 57, 230
SspMI CTAG 2 cut(s) 186, 552
StyD4I CCNGG 1 cut(s) 356
TaaI ACNGT 3 cut(s) 161, 319, 708
TaiI ACGT 1 cut(s) 300
TaqI TCGA 2 cut(s) 5, 691
TasI AATT 3 cut(s) 174, 246, 329
TatI WGTACW 1 cut(s) 314
TfiI GAWTC 1 cut(s) 497
TscAI CASTG 3 cut(s) 166, 324, 711
TseFI GTSAC 1 cut(s) 272
Tsp45I GTSAC 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 3 cut(s) 166, 324, 711
VpaK11BI GGWCC 1 cut(s) 235
XceI RCATGY 2 cut(s) 283, 641
XspI CTAG 2 cut(s) 186, 552
Zsp2I ATGCAT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.