Rh6DG492800

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000825
Physical Location & Seq
Forward (+)
65214646 .. 65217097
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG492800.1

Sequence Viewer

Length: 795 bp
ATGTCGAGGAGGCAAGCGAATGCGAACCTATCTCGGCGACTGGGAGATGCCGGAGGCGGTTGGCTCTCAGGCTCGTCCTATTCTAAATCAAGGTCTTCTCCCATCTTATATGTCGCTCTCATTGCCGTGGGAGCATTGCTTATTCTTGCCTATTCACACAGTGGCAGAGGTGGAATTGGTAATACTAGAGGGGCTATTAGCAGGGTTCAGGGTGATTACTCATGCACAGCGGAGGTCCAGCGAGCAATTCCTGTTTTGAAGAAAGCATATGGTGACAGCATGCATAAGGTTTTGCACGTTGGCCCTGATACTTGTACAGTGGTTTATAAATTGTGTAGTGAGGAGGAAACTGAAGCCTGGGGTATGGATCCGTACAATGTAGAGGATGCTGATAGAAGTTGCAAAGCTCTTGTGCGCAAAGGCATAGTGCGTGTTGATGATATCAAGTATCCACTTCCATACAGGCCAAAATCATTTTCTGTTGTCATTGTTTCGGATTCTTTGGATTATTTGTCTGAAAGATACCTCAACAAGACCCTTCCGGATCTGGCTAGGGTATCAGCTGATGGTGTTGTAATCTTTACAGGCTCTCCTGGCAAGCAGAGAACTAAAGCAGCTGAGGTCTCCAAATTTGGTAGGGCGGCTAAATTGAGGAACACAGCCTGGTGGACTAAGTTTTTTGGGCAGGTTAGCTTAGAAGAGAACGAAGGTGCTGGCAAGAAGTTTAATATTGCTGCAACAAAGATGTCATTTGTCTCAAATTGTCAAGTTTTCCACCTCAGGTCATTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

264

Amino Acids

28.92

Weight (kDa)

9.58

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
Acc16I TGCGCA 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 676
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 3 cut(s) 57, 230, 641
AclWI GGATC 3 cut(s) 362, 375, 552
AcsI RAATTY 1 cut(s) 629
AcuI CTGAAG 1 cut(s) 372
AdeI CACNNNGTG 1 cut(s) 161
AfaI GTAC 2 cut(s) 316, 374
AgsI TTSAA 2 cut(s) 259, 790
AjnI CCWGG 3 cut(s) 356, 592, 662
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AluBI AGCT 4 cut(s) 407, 563, 617, 693
AluI AGCT 4 cut(s) 407, 563, 617, 693
Alw26I GTCTC 2 cut(s) 628, 760
AlwI GGATC 3 cut(s) 362, 375, 552
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 2 cut(s) 301, 464
ApeKI GCWGC 2 cut(s) 614, 734
ApoI RAATTY 1 cut(s) 629
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 2 cut(s) 235, 302
AsuHPI GGTGA 2 cut(s) 224, 284
AvaII GGWCC 1 cut(s) 235
AxyI CCTNAGG 1 cut(s) 779
BamHI GGATCC 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 618
BbvI GCAGC 2 cut(s) 626, 721
BccI CCATC 2 cut(s) 110, 560
BceAI ACGGC 1 cut(s) 110
BciT130I CCWGG 3 cut(s) 358, 594, 664
BciVI GTATCC 1 cut(s) 459
BcoDI GTCTC 2 cut(s) 628, 760
BfaI CTAG 2 cut(s) 186, 552
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 1 cut(s) 459
BisI GCNGC 3 cut(s) 615, 642, 735
BlsI GCNGC 3 cut(s) 616, 643, 736
Bme1390I CCNGG 3 cut(s) 358, 594, 664
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 235, 302
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 3 cut(s) 358, 594, 664
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 2 cut(s) 37, 376
BmuI ACTGGG 1 cut(s) 50
BpiI GAAGAC 1 cut(s) 87
Bpu10I CCTNAGC 1 cut(s) 618
BsaI GGTCTC 1 cut(s) 628
BsaJI CCNNGG 2 cut(s) 126, 357
BsaWI WCCGGW 1 cut(s) 541
BsaXI ACNNNNNCTCC 2 cut(s) 574, 604
Bse1I ACTGG 1 cut(s) 45
Bse21I CCTNAGG 1 cut(s) 779
Bse3DI GCAATG 2 cut(s) 120, 134
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 3 cut(s) 358, 594, 664
BseDI CCNNGG 2 cut(s) 126, 357
BseGI GGATG 1 cut(s) 391
BseMI GCAATG 2 cut(s) 120, 134
BseMII CTCAG 3 cut(s) 81, 609, 793
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 2 cut(s) 22, 356
BseXI GCAGC 2 cut(s) 626, 721
BshFI GGCC 2 cut(s) 303, 466
BsiSI CCGG 2 cut(s) 51, 542
BsmAI GTCTC 2 cut(s) 628, 760
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 2 cut(s) 303, 466
Bso31I GGTCTC 1 cut(s) 628
Bsp13I TCCGGA 1 cut(s) 541
Bsp1407I TGTACA 1 cut(s) 314
Bsp143I GATC 2 cut(s) 367, 544
BspACI CCGC 3 cut(s) 57, 230, 641
BspANI GGCC 2 cut(s) 303, 466
BspCNI CTCAG 3 cut(s) 80, 610, 792
BspEI TCCGGA 1 cut(s) 541
BspLI GGNNCC 1 cut(s) 369
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 3 cut(s) 362, 375, 552
BspTNI GGTCTC 1 cut(s) 628
BsrDI GCAATG 2 cut(s) 120, 134
BsrGI TGTACA 1 cut(s) 314
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 126, 357
BssMI GATC 2 cut(s) 367, 544
Bst2UI CCWGG 3 cut(s) 358, 594, 664
Bst4CI ACNGT 2 cut(s) 161, 319
Bst6I CTCTTC 1 cut(s) 693
BstAUI TGTACA 1 cut(s) 314
BstC8I GCNNGC 5 cut(s) 15, 243, 281, 599, 715
BstDEI CTNAG 5 cut(s) 67, 618, 672, 694, 779
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 391
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 2 cut(s) 370, 547
BstMAI GTCTC 2 cut(s) 628, 760
BstMBI GATC 2 cut(s) 367, 544
BstMWI GCNNNNNNNGC 3 cut(s) 122, 131, 594
BstNI CCWGG 3 cut(s) 358, 594, 664
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 3 cut(s) 356, 592, 662
BstV1I GCAGC 2 cut(s) 626, 721
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 2 cut(s) 367, 544
BstYI RGATCY 2 cut(s) 367, 544
Bsu36I CCTNAGG 1 cut(s) 779
BsuI GTATCC 1 cut(s) 459
BsuRI GGCC 2 cut(s) 303, 466
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 2 cut(s) 166, 324
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 5 cut(s) 15, 243, 281, 599, 715
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 2 cut(s) 235, 302
Csp6I GTAC 2 cut(s) 315, 373
CviAII CATG 2 cut(s) 222, 280
CviQI GTAC 2 cut(s) 315, 373
DdeI CTNAG 5 cut(s) 67, 618, 672, 694, 779
DpnI GATC 2 cut(s) 369, 546
DpnII GATC 2 cut(s) 367, 544
DraIII CACNNNGTG 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 628
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 372
Eco81I CCTNAGG 1 cut(s) 779
EcoRII CCWGG 3 cut(s) 356, 592, 662
EcoRV GATATC 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 285
FaeI CATG 2 cut(s) 225, 283
FatI CATG 2 cut(s) 221, 279
FauNDI CATATG 1 cut(s) 268
Fnu4HI GCNGC 3 cut(s) 615, 642, 735
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 3 cut(s) 615, 642, 735
FspBI CTAG 2 cut(s) 186, 552
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 1 cut(s) 416
GluI GCNGC 3 cut(s) 615, 642, 735
HaeIII GGCC 2 cut(s) 303, 466
HapII CCGG 2 cut(s) 51, 542
HhaI GCGC 1 cut(s) 417
Hin1II CATG 2 cut(s) 225, 283
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HinfI GANTC 1 cut(s) 497
HpaII CCGG 2 cut(s) 51, 542
HphI GGTGA 2 cut(s) 224, 284
Hpy166II GTNNAC 1 cut(s) 669
Hpy188I TCNGA 2 cut(s) 496, 517
Hpy188III TCNNGA 1 cut(s) 542
Hpy8I GTNNAC 1 cut(s) 669
HpyAV CCTTC 2 cut(s) 548, 701
HpyCH4III ACNGT 2 cut(s) 161, 319
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 5 cut(s) 225, 283, 295, 402, 737
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 131, 594
HpyF3I CTNAG 5 cut(s) 67, 618, 672, 694, 779
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 2 cut(s) 225, 283
HspAI GCGC 1 cut(s) 415
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 2 cut(s) 367, 544
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 2 cut(s) 626, 721
LweI GCATC 2 cut(s) 37, 376
MaeI CTAG 2 cut(s) 186, 552
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 272
MalI GATC 2 cut(s) 369, 546
MboI GATC 2 cut(s) 367, 544
MboII GAAGA 3 cut(s) 87, 271, 710
MfeI CAATTG 1 cut(s) 790
MflI RGATCY 2 cut(s) 367, 544
MluCI AATT 7 cut(s) 174, 246, 329, 629, 647, 760, 790
Mph1103I ATGCAT 1 cut(s) 285
MroI TCCGGA 1 cut(s) 541
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 1 cut(s) 125
MspA1I CMGCKG 3 cut(s) 230, 563, 617
MspI CCGG 2 cut(s) 51, 542
MspR9I CCNGG 3 cut(s) 358, 594, 664
MunI CAATTG 1 cut(s) 790
Mva1269I GAATGC 1 cut(s) 25
MvaI CCWGG 3 cut(s) 358, 594, 664
MwoI GCNNNNNNNGC 3 cut(s) 122, 131, 594
NdeI CATATG 1 cut(s) 268
NdeII GATC 2 cut(s) 367, 544
NlaIII CATG 2 cut(s) 225, 283
NlaIV GGNNCC 1 cut(s) 369
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 272
NsbI TGCGCA 1 cut(s) 416
NsiI ATGCAT 1 cut(s) 285
NspI RCATGY 1 cut(s) 283
PaeI GCATGC 1 cut(s) 283
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 497
PkrI GCNGC 3 cut(s) 616, 643, 736
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 3 cut(s) 356, 592, 662
PspGI CCWGG 3 cut(s) 356, 592, 662
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 2 cut(s) 235, 302
PsuI RGATCY 2 cut(s) 367, 544
PvuII CAGCTG 2 cut(s) 563, 617
RsaI GTAC 2 cut(s) 316, 374
RsaNI GTAC 2 cut(s) 315, 373
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 3 cut(s) 615, 642, 735
Sau3AI GATC 2 cut(s) 367, 544
Sau96I GGNCC 2 cut(s) 235, 302
ScrFI CCNGG 3 cut(s) 358, 594, 664
SfaNI GCATC 2 cut(s) 37, 376
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 125
SphI GCATGC 1 cut(s) 283
Sse9I AATT 7 cut(s) 174, 246, 329, 629, 647, 760, 790
SsiI CCGC 3 cut(s) 57, 230, 641
SspI AATATT 1 cut(s) 730
SspMI CTAG 2 cut(s) 186, 552
StyD4I CCNGG 3 cut(s) 356, 592, 662
TaaI ACNGT 2 cut(s) 161, 319
TaiI ACGT 1 cut(s) 300
TaqI TCGA 1 cut(s) 5
TasI AATT 7 cut(s) 174, 246, 329, 629, 647, 760, 790
TatI WGTACW 1 cut(s) 314
TauI GCSGC 1 cut(s) 644
TfiI GAWTC 1 cut(s) 497
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 2 cut(s) 166, 324
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 2 cut(s) 614, 734
Tsp45I GTSAC 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 2 cut(s) 166, 324
VpaK11BI GGWCC 1 cut(s) 235
XapI RAATTY 1 cut(s) 629
XceI RCATGY 1 cut(s) 283
XspI CTAG 2 cut(s) 186, 552
Zsp2I ATGCAT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.