Rorug05G0524000

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000825
Physical Location & Seq
Reverse (-)
71005238 .. 71012198
6961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0524000.1

Sequence Viewer

Length: 1536 bp
ATGGCCTCTTCACTGCGACTCCCGCTCTCGCTTTGGCTTCCAAAACACCCAAACCAGAGCTTCCATTTTTCAAACCCAAAAATACCCTCAAGATTGAACCTTATTTACTGCTGCTCCTCCTCCGCTGCCACTGCTCAACAAACCTTAACCCACACTGAAAAATGGCAATCCGTTCGAAAGAAGAAAGTGGTTCTCCGGGTCGGGTATGTGGGCACCGATTACCGAGGTCTGCAAATGCAAAGAGATGACCCTTCAATATCTACTATTGAAAAGGAACTGGAAACTGCCATATTCAAGGCCGGTGGTATTCGCGAGAGTAACTTCGGGAACCTGAACAAGATTGCGTGGGCCAGAAGCAGCAGAACCGATAAGGGAGTTCACTCCTTGGCGACAATGATATCATTGAAAATGGAAATCCCTGAAAATGCGTGGGACGGAGACCCGTATGGCATTGCTCTTGTGAATCACATCAACTGTTACCTTCCTCACAATATCAAAATTTTTAGTGTTTTACCTGCACAGAGGAGCTTTGATCCCAGAAAGGAGTGTAATCTCCGTATGTATTCATACCTCCTTCCTGCTGATGTTATTGGAATCAAAAGTCACTTTAGCACAGCTGAAATTGATGATCACATTGCAGACTTCAACGATATACTAAATGCTTTCGAGGGTGAACATCCTTTTCATAACTATACAGTACGATCTAAATACAGGAAAAAACTTCCTGGGAAGAAATCACCTATATACTGCAATATGTCAAAAACAGAAAGATCAACTGTTGAGGCATCAGCCTCCGAGCTTGAGGGAAGTGACGATGAAGATGAGTTCGAGGGAGAAAATACAACAGAATATGAGGTACTGAACCAAAATTCTTTCCTGTCCAGCAATGGGAATGAAAACTTACTGAAAGCATGCAAGAAGCAGGAAGATGTTCTGAAGGAGAAAAGTACTGATTCAGTTATCCGTGCAAAATGGTTATACGAACCTGATGAGAGCGATAGAATCGGTGCTTCTCATTTCAGAAAGATATTTCGCTGTTCTTGTGGAAAGCTTGAAAAATCGCTGGCACATGAGTATGTTGAGCTCTCCATATGGGGAGAGTCTTTCATGTTACATCAAATTCGCAAAATGGTTGGAACTGCTGTGGCCATAAAGCGCAAGTTACTCCCAAGAGATATTCTGACATTATCACTGGTCAAGTTCTCACGGATAATCCTACCCCTTGCTCCATCTGAAGTTTTGATCTTAAGAGGAAACAGATTTTCAATTAGAGAAAGACCTGGAAATGTAACAAGGCCTGAAATGATTGCAATGGTTGAATCAGAAGAAATTATCAAACAGGTTGATGAGTTCTTCACATCTGTAATGTTGCCTCAAGTTGCAAAGTTCTTAGAACCTTCAGGATATCCTTGGCAAGAATGGATTGAAAACTTGGATAGAAACACAGGTATTCCAGATATAGAATTGGATGAAGTTAGAAATGCTTGGAAAGTGTGGAAGGAAAGGTTTGAGAGTGATGCATCAATCCTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

511

Amino Acids

58.48

Weight (kDa)

7.2

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AccB1I GGYRCC 1 cut(s) 212
AccBSI CCGCTC 1 cut(s) 25
AccII CGCG 1 cut(s) 312
AciI CCGC 2 cut(s) 23, 123
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 1 cut(s) 1146
AcsI RAATTY 3 cut(s) 498, 868, 1119
AcuI CTGAAG 3 cut(s) 956, 1254, 1383
AfaI GTAC 3 cut(s) 699, 858, 949
AfiI CCNNNNNNNGG 1 cut(s) 888
AflII CTTAAG 1 cut(s) 1246
AjnI CCWGG 2 cut(s) 724, 1279
AluBI AGCT 6 cut(s) 60, 528, 617, 799, 1051, 1084
AluI AGCT 6 cut(s) 60, 528, 617, 799, 1051, 1084
Alw21I GWGCWC 1 cut(s) 1086
Alw26I GTCTC 1 cut(s) 432
AlwI GGATC 1 cut(s) 527
AoxI GGCC 5 cut(s) 3, 297, 348, 1146, 1295
ApeKI GCWGC 3 cut(s) 111, 125, 357
ApoI RAATTY 3 cut(s) 498, 868, 1119
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 1 cut(s) 1158
AspS9I GGNCC 1 cut(s) 348
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 683, 729
AsuII TTCGAA 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 215
BalI TGGCCA 1 cut(s) 1148
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 1086
Bbv12I GWGCWC 1 cut(s) 1086
BbvI GCAGC 3 cut(s) 98, 112, 369
BccI CCATC 1 cut(s) 1237
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 2 cut(s) 726, 1281
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 432
BfrI CTTAAG 1 cut(s) 1246
BfuAI ACCTGC 1 cut(s) 523
BisI GCNGC 3 cut(s) 112, 126, 358
BlsI GCNGC 3 cut(s) 113, 127, 359
BmcAI AGTACT 1 cut(s) 949
Bme1390I CCNGG 3 cut(s) 197, 726, 1281
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 2 cut(s) 214, 329
BmrFI CCNGG 3 cut(s) 197, 726, 1281
BmsI GCATC 3 cut(s) 794, 1507, 1529
Bpu14I TTCGAA 1 cut(s) 175
BpuEI CTTGAG 3 cut(s) 73, 821, 1359
BpuMI CCSGG 1 cut(s) 197
BsaI GGTCTC 1 cut(s) 432
BsaJI CCNNGG 4 cut(s) 223, 384, 725, 1409
BsaXI ACNNNNNCTCC 3 cut(s) 33, 98, 128
Bsc4I CCNNNNNNNGG 1 cut(s) 888
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 2 cut(s) 282, 1197
Bse3DI GCAATG 4 cut(s) 450, 633, 892, 1317
BseBI CCWGG 2 cut(s) 726, 1281
BseDI CCNNGG 4 cut(s) 223, 384, 725, 1409
BseGI GGATG 2 cut(s) 676, 1474
BseLI CCNNNNNNNGG 1 cut(s) 888
BseMI GCAATG 4 cut(s) 450, 633, 892, 1317
BseNI ACTGG 2 cut(s) 282, 1197
BseRI GAGGAG 3 cut(s) 106, 109, 538
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 3 cut(s) 98, 112, 369
BsgI GTGCAG 1 cut(s) 501
Bsh1236I CGCG 1 cut(s) 312
BshFI GGCC 5 cut(s) 5, 299, 350, 1148, 1297
BshNI GGYRCC 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 1086
BsiSI CCGG 2 cut(s) 196, 300
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 888
BsmAI GTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 446
BsnI GGCC 5 cut(s) 5, 299, 350, 1148, 1297
Bso31I GGTCTC 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 175
Bsp1286I GDGCHC 2 cut(s) 215, 1086
Bsp143I GATC 5 cut(s) 532, 628, 701, 770, 1242
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 2 cut(s) 23, 123
BspANI GGCC 5 cut(s) 5, 299, 350, 1148, 1297
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 214, 329
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 1 cut(s) 527
BspT104I TTCGAA 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 212
BspTI CTTAAG 1 cut(s) 1246
BspTNI GGTCTC 1 cut(s) 432
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 4 cut(s) 450, 633, 892, 1317
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 2 cut(s) 282, 1197
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 4 cut(s) 223, 384, 725, 1409
BssMI GATC 5 cut(s) 532, 628, 701, 770, 1242
BssT1I CCWWGG 2 cut(s) 384, 1409
Bst2UI CCWGG 2 cut(s) 726, 1281
Bst4CI ACNGT 3 cut(s) 476, 697, 778
Bst6I CTCTTC 1 cut(s) 13
BstAFI CTTAAG 1 cut(s) 1246
BstBI TTCGAA 1 cut(s) 175
BstC8I GCNNGC 2 cut(s) 913, 1065
BstDEI CTNAG 1 cut(s) 1390
BstF5I GGATG 2 cut(s) 676, 1474
BstFNI CGCG 1 cut(s) 312
BstHHI GCGC 1 cut(s) 1158
BstKTI GATC 5 cut(s) 535, 631, 704, 773, 1245
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 5 cut(s) 532, 628, 701, 770, 1242
BstMWI GCNNNNNNNGC 2 cut(s) 22, 131
BstNI CCWGG 2 cut(s) 726, 1281
BstNSI RCATGY 1 cut(s) 915
BstSCI CCNGG 3 cut(s) 195, 724, 1279
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 3 cut(s) 98, 112, 369
BsuRI GGCC 5 cut(s) 5, 299, 350, 1148, 1297
BtsCI GGATG 2 cut(s) 676, 1474
BtsI GCAGTG 2 cut(s) 11, 129
BtsIMutI CAGTG 4 cut(s) 11, 129, 153, 1190
BtuMI TCGCGA 1 cut(s) 312
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 2 cut(s) 913, 1065
CfoI GCGC 1 cut(s) 1158
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 348
Csp6I GTAC 3 cut(s) 698, 857, 948
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 3 cut(s) 912, 1070, 1108
CviQI GTAC 3 cut(s) 698, 857, 948
DdeI CTNAG 1 cut(s) 1390
DpnI GATC 5 cut(s) 534, 630, 703, 772, 1244
DpnII GATC 5 cut(s) 532, 628, 701, 770, 1242
EaeI YGGCCR 1 cut(s) 1146
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 1084
Eco130I CCWWGG 2 cut(s) 384, 1409
Eco147I AGGCCT 1 cut(s) 1297
Eco24I GRGCYC 1 cut(s) 1086
Eco31I GGTCTC 1 cut(s) 432
Eco32I GATATC 2 cut(s) 399, 1406
Eco53kI GAGCTC 1 cut(s) 1084
Eco57I CTGAAG 3 cut(s) 956, 1254, 1383
EcoICRI GAGCTC 1 cut(s) 1084
EcoRII CCWGG 2 cut(s) 724, 1279
EcoRV GATATC 2 cut(s) 399, 1406
EcoT14I CCWWGG 2 cut(s) 384, 1409
EcoT22I ATGCAT 1 cut(s) 1522
EcoT38I GRGCYC 1 cut(s) 1086
ErhI CCWWGG 2 cut(s) 384, 1409
FaeI CATG 3 cut(s) 915, 1073, 1111
FaqI GGGAC 1 cut(s) 446
FatI CATG 3 cut(s) 911, 1069, 1107
FauI CCCGC 1 cut(s) 30
FauNDI CATATG 1 cut(s) 1091
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 3 cut(s) 112, 126, 358
FokI GGATG 2 cut(s) 663, 1481
FriOI GRGCYC 1 cut(s) 1086
Fsp4HI GCNGC 3 cut(s) 112, 126, 358
GlaI GCGC 1 cut(s) 1157
GluI GCNGC 3 cut(s) 112, 126, 358
HaeIII GGCC 5 cut(s) 5, 299, 350, 1148, 1297
HapII CCGG 2 cut(s) 196, 300
HhaI GCGC 1 cut(s) 1158
Hin1II CATG 3 cut(s) 915, 1073, 1111
Hin6I GCGC 1 cut(s) 1156
HinP1I GCGC 1 cut(s) 1156
HindIII AAGCTT 1 cut(s) 1049
HinfI GANTC 7 cut(s) 18, 463, 594, 953, 1002, 1100, 1319
HpaII CCGG 2 cut(s) 196, 300
HphI GGTGA 2 cut(s) 683, 729
Hpy166II GTNNAC 2 cut(s) 379, 674
Hpy188I TCNGA 6 cut(s) 796, 936, 1022, 1182, 1234, 1324
Hpy188III TCNNGA 5 cut(s) 90, 311, 325, 1401, 1454
Hpy8I GTNNAC 2 cut(s) 379, 674
HpyAV CCTTC 6 cut(s) 261, 491, 584, 931, 1407, 1492
HpyCH4III ACNGT 3 cut(s) 476, 697, 778
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 131
HpyF3I CTNAG 1 cut(s) 1390
Hsp92II CATG 3 cut(s) 915, 1073, 1111
HspAI GCGC 1 cut(s) 1156
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 5 cut(s) 532, 628, 701, 770, 1242
LmnI GCTCC 3 cut(s) 119, 525, 1231
Lsp1109I GCAGC 3 cut(s) 98, 112, 369
LweI GCATC 3 cut(s) 794, 1507, 1529
MaeIII GTNAC 7 cut(s) 317, 476, 602, 809, 1110, 1161, 1288
MalI GATC 5 cut(s) 534, 630, 703, 772, 1244
MbiI CCGCTC 1 cut(s) 25
MboI GATC 5 cut(s) 532, 628, 701, 770, 1242
MboII GAAGA 6 cut(s) 193, 742, 830, 938, 1337, 1345
MhlI GDGCHC 2 cut(s) 215, 1086
MlsI TGGCCA 1 cut(s) 1148
MluCI AATT 7 cut(s) 498, 621, 868, 1119, 1266, 1329, 1463
MluNI TGGCCA 1 cut(s) 1148
MlyI GAGTC 2 cut(s) 12, 1109
MmeI TCCRAC 1 cut(s) 1114
Mox20I TGGCCA 1 cut(s) 1148
Mph1103I ATGCAT 1 cut(s) 1522
MroXI GAANNNNTTC 1 cut(s) 930
MscI TGGCCA 1 cut(s) 1148
MseI TTAA 2 cut(s) 146, 1247
MslI CAYNNNNRTG 1 cut(s) 1074
Msp20I TGGCCA 1 cut(s) 1148
MspA1I CMGCKG 2 cut(s) 125, 617
MspCI CTTAAG 1 cut(s) 1246
MspI CCGG 2 cut(s) 196, 300
MspR9I CCNGG 3 cut(s) 197, 726, 1281
MvaI CCWGG 2 cut(s) 726, 1281
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 2 cut(s) 22, 131
NciI CCSGG 1 cut(s) 197
NdeI CATATG 1 cut(s) 1091
NdeII GATC 5 cut(s) 532, 628, 701, 770, 1242
NlaIII CATG 3 cut(s) 915, 1073, 1111
NlaIV GGNNCC 2 cut(s) 214, 329
NmuCI GTSAC 2 cut(s) 602, 809
NruI TCGCGA 1 cut(s) 312
NsiI ATGCAT 1 cut(s) 1522
NspI RCATGY 1 cut(s) 915
NspV TTCGAA 1 cut(s) 175
PaeI GCATGC 1 cut(s) 915
PceI AGGCCT 1 cut(s) 1297
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 5 cut(s) 463, 594, 953, 1002, 1319
PkrI GCNGC 3 cut(s) 113, 127, 359
PleI GAGTC 2 cut(s) 12, 1108
PpsI GAGTC 2 cut(s) 12, 1108
Psp124BI GAGCTC 1 cut(s) 1086
Psp6I CCWGG 2 cut(s) 724, 1279
PspGI CCWGG 2 cut(s) 724, 1279
PspN4I GGNNCC 2 cut(s) 214, 329
PspPI GGNCC 1 cut(s) 348
PsrI GAACNNNNNNTAC 2 cut(s) 89, 121
PvuII CAGCTG 1 cut(s) 617
RruI TCGCGA 1 cut(s) 312
RsaI GTAC 3 cut(s) 699, 858, 949
RsaNI GTAC 3 cut(s) 698, 857, 948
RseI CAYNNNNRTG 1 cut(s) 1074
SacI GAGCTC 1 cut(s) 1086
SaqAI TTAA 2 cut(s) 146, 1247
SatI GCNGC 3 cut(s) 112, 126, 358
Sau3AI GATC 5 cut(s) 532, 628, 701, 770, 1242
Sau96I GGNCC 1 cut(s) 348
ScaI AGTACT 1 cut(s) 949
SchI GAGTC 2 cut(s) 12, 1109
ScrFI CCNGG 3 cut(s) 197, 726, 1281
SduI GDGCHC 2 cut(s) 215, 1086
SfaNI GCATC 3 cut(s) 794, 1507, 1529
SfuI TTCGAA 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 1074
SmlI CTYRAG 4 cut(s) 88, 800, 1246, 1374
SmoI CTYRAG 4 cut(s) 88, 800, 1246, 1374
SphI GCATGC 1 cut(s) 915
Sse9I AATT 7 cut(s) 498, 621, 868, 1119, 1266, 1329, 1463
SseBI AGGCCT 1 cut(s) 1297
SsiI CCGC 2 cut(s) 23, 123
SstI GAGCTC 1 cut(s) 1086
StuI AGGCCT 1 cut(s) 1297
StyD4I CCNGG 3 cut(s) 195, 724, 1279
StyI CCWWGG 2 cut(s) 384, 1409
TaaI ACNGT 3 cut(s) 476, 697, 778
TaqI TCGA 3 cut(s) 175, 666, 828
TasI AATT 7 cut(s) 498, 621, 868, 1119, 1266, 1329, 1463
TatI WGTACW 1 cut(s) 947
TfiI GAWTC 5 cut(s) 463, 594, 953, 1002, 1319
Tru1I TTAA 2 cut(s) 146, 1247
Tru9I TTAA 2 cut(s) 146, 1247
TscAI CASTG 4 cut(s) 18, 136, 160, 1197
TseFI GTSAC 2 cut(s) 602, 809
TseI GCWGC 3 cut(s) 111, 125, 357
Tsp45I GTSAC 2 cut(s) 602, 809
TspDTI ATGAA 6 cut(s) 555, 674, 831, 909, 1096, 1485
TspGWI ACGGA 5 cut(s) 160, 450, 545, 953, 1222
TspRI CASTG 4 cut(s) 18, 136, 160, 1197
Vha464I CTTAAG 1 cut(s) 1246
XapI RAATTY 3 cut(s) 498, 868, 1119
XceI RCATGY 1 cut(s) 915
XmnI GAANNNNTTC 1 cut(s) 930
ZrmI AGTACT 1 cut(s) 949
Zsp2I ATGCAT 1 cut(s) 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.