RLG00000031939

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000825
Physical Location & Seq
Forward (+)
9681084 .. 9682431
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031939

Sequence Viewer

Length: 489 bp
ATGTTAGATCTCTTGCATTTGCGAAGCACAATTTACTTAATCTACCTGCAGGTTTTCCTGGAAGAACTGTGCCTTTTGAGTCACTTCAAGCACTTGGATGCCATGTTGATGCAGTTTGCCCCAAAGAGAAAAGCTAGCGATGAACATATAGATATAGATACAGGATCTCCACCTAATGAATCTTGTGCTCAACTTCCGTGGCAAACAGATTTAGTAATGGAACTGCAGTTCAGCTCAGTTCTAAATTTATCGATTGAGGAGGCAAGTGAATGCGAACCGTTCTCGGGAGCATTGCTTATTCTTGCCTATTCACACAGTTGCAAAGGTGGACTTGGTAATATTAGAAGGGCTACTAGCAGGGTTCAAGGTGATTACTCATGCACAACGGAGGTCCAGCAAGCAATTCATGTTTTGAAGAAAGCATATGGTGACAGCATGCATAAGGTTTTACATGTTGGCCCTAATACTTATACTGTGCATTTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

163

Amino Acids

18.29

Weight (kDa)

5.79

Isoelectric Point (pI)

61.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 40, 54
AclWI GGATC 1 cut(s) 172
AcsI RAATTY 1 cut(s) 244
AfiI CCNNNNNNNGG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 3 cut(s) 88, 365, 415
AjnI CCWGG 1 cut(s) 57
AloI GAACNNNNNNTCC 2 cut(s) 212, 244
AluBI AGCT 2 cut(s) 134, 234
AluI AGCT 2 cut(s) 134, 234
Alw21I GWGCWC 1 cut(s) 190
AlwI GGATC 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 283
AoxI GGCC 1 cut(s) 457
ApoI RAATTY 1 cut(s) 244
AspS9I GGNCC 2 cut(s) 391, 458
AsuHPI GGTGA 2 cut(s) 380, 440
AsuNHI GCTAGC 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 283
AvaII GGWCC 1 cut(s) 391
Bbv12I GWGCWC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 59
BfaI CTAG 2 cut(s) 135, 354
BfmI CTRYAG 2 cut(s) 47, 224
BfuAI ACCTGC 2 cut(s) 40, 54
BglII AGATCT 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 391
BmeT110I CYCGRG 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 391, 458
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 2 cut(s) 88, 99
BmtI GCTAGC 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 290
BseBI CCWGG 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 251
BseDI CCNNGG 1 cut(s) 197
BseGI GGATG 1 cut(s) 103
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 272
BshFI GGCC 1 cut(s) 459
BshVI ATCGAT 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 190
BsiHKCI CYCGRG 1 cut(s) 283
BslI CCNNNNNNNGG 1 cut(s) 284
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 459
BsoBI CYCGRG 1 cut(s) 283
Bsp1286I GDGCHC 1 cut(s) 190
Bsp143I GATC 2 cut(s) 7, 164
BspANI GGCC 1 cut(s) 459
BspCNI CTCAG 1 cut(s) 248
BspDI ATCGAT 1 cut(s) 251
BspMAI CTGCAG 2 cut(s) 51, 228
BspMI ACCTGC 2 cut(s) 40, 54
BspOI GCTAGC 1 cut(s) 138
BspPI GGATC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 197
BssMI GATC 2 cut(s) 7, 164
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 4 cut(s) 69, 279, 317, 475
BstC8I GCNNGC 3 cut(s) 136, 399, 437
BstDEI CTNAG 1 cut(s) 235
BstDSI CCRYGG 1 cut(s) 197
BstF5I GGATG 1 cut(s) 103
BstKTI GATC 2 cut(s) 10, 167
BstMBI GATC 2 cut(s) 7, 164
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 2 cut(s) 439, 455
BstSCI CCNGG 1 cut(s) 57
BstSFI CTRYAG 2 cut(s) 47, 224
BstX2I RGATCY 2 cut(s) 7, 164
BstYI RGATCY 2 cut(s) 7, 164
Bsu15I ATCGAT 1 cut(s) 251
BsuRI GGCC 1 cut(s) 459
BsuTUI ATCGAT 1 cut(s) 251
BtgI CCRYGG 1 cut(s) 197
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 103
BveI ACCTGC 2 cut(s) 40, 54
Cac8I GCNNGC 3 cut(s) 136, 399, 437
Cfr13I GGNCC 2 cut(s) 391, 458
ClaI ATCGAT 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 179, 214
CviAII CATG 5 cut(s) 103, 378, 407, 436, 452
CviJI RGCY 4 cut(s) 134, 234, 350, 459
CviKI_1 RGCY 4 cut(s) 134, 234, 350, 459
DdeI CTNAG 1 cut(s) 235
DpnI GATC 2 cut(s) 9, 166
DpnII GATC 2 cut(s) 7, 164
Eco47I GGWCC 1 cut(s) 391
Eco88I CYCGRG 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 57
EcoT22I ATGCAT 1 cut(s) 441
FaeI CATG 5 cut(s) 106, 381, 410, 439, 455
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FatI CATG 5 cut(s) 102, 377, 406, 435, 451
FauNDI CATATG 1 cut(s) 424
FokI GGATG 1 cut(s) 110
FspBI CTAG 2 cut(s) 135, 354
HaeIII GGCC 1 cut(s) 459
Hin1II CATG 5 cut(s) 106, 381, 410, 439, 455
HinfI GANTC 2 cut(s) 79, 179
HphI GGTGA 2 cut(s) 380, 440
Hpy166II GTNNAC 1 cut(s) 329
Hpy188III TCNNGA 1 cut(s) 285
Hpy8I GTNNAC 1 cut(s) 329
HpyAV CCTTC 1 cut(s) 339
HpyCH4III ACNGT 4 cut(s) 69, 279, 317, 475
HpyCH4V TGCA 8 cut(s) 16, 49, 112, 226, 321, 381, 439, 478
HpyF3I CTNAG 1 cut(s) 235
Hsp92II CATG 5 cut(s) 106, 381, 410, 439, 455
Kzo9I GATC 2 cut(s) 7, 164
LmnI GCTCC 1 cut(s) 287
LpnPI CCDG 7 cut(s) 35, 44, 59, 71, 147, 343, 407
LweI GCATC 2 cut(s) 88, 99
MaeI CTAG 2 cut(s) 135, 354
MaeIII GTNAC 2 cut(s) 80, 428
MalI GATC 2 cut(s) 9, 166
MboI GATC 2 cut(s) 7, 164
MboII GAAGA 2 cut(s) 74, 427
MflI RGATCY 2 cut(s) 7, 164
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 3 cut(s) 30, 244, 402
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 3 cut(s) 250, 253, 382
Mph1103I ATGCAT 1 cut(s) 441
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 2 cut(s) 96, 107
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 1 cut(s) 59
NdeI CATATG 1 cut(s) 424
NdeII GATC 2 cut(s) 7, 164
NheI GCTAGC 1 cut(s) 134
NlaIII CATG 5 cut(s) 106, 381, 410, 439, 455
NmuCI GTSAC 2 cut(s) 80, 428
NsiI ATGCAT 1 cut(s) 441
NspI RCATGY 2 cut(s) 439, 455
PaeI GCATGC 1 cut(s) 439
PciI ACATGT 1 cut(s) 451
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 179
PfoI TCCNGGA 1 cut(s) 57
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PscI ACATGT 1 cut(s) 451
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspPI GGNCC 2 cut(s) 391, 458
PstI CTGCAG 2 cut(s) 51, 228
PsuI RGATCY 2 cut(s) 7, 164
RseI CAYNNNNRTG 2 cut(s) 96, 107
SaqAI TTAA 1 cut(s) 38
Sau3AI GATC 2 cut(s) 7, 164
Sau96I GGNCC 2 cut(s) 391, 458
SbfI CCTGCAGG 1 cut(s) 51
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 59
SdaI CCTGCAGG 1 cut(s) 51
SduI GDGCHC 1 cut(s) 190
SetI ASST 9 cut(s) 48, 54, 136, 175, 236, 328, 370, 393, 447
SfaNI GCATC 2 cut(s) 88, 99
SfcI CTRYAG 2 cut(s) 47, 224
SinI GGWCC 1 cut(s) 391
SmiMI CAYNNNNRTG 2 cut(s) 96, 107
SphI GCATGC 1 cut(s) 439
Sse8387I CCTGCAGG 1 cut(s) 51
Sse9I AATT 3 cut(s) 30, 244, 402
SspI AATATT 1 cut(s) 340
SspMI CTAG 2 cut(s) 135, 354
StyD4I CCNGG 1 cut(s) 57
TaaI ACNGT 4 cut(s) 69, 279, 317, 475
TaqI TCGA 1 cut(s) 251
TasI AATT 3 cut(s) 30, 244, 402
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TseFI GTSAC 2 cut(s) 80, 428
Tsp45I GTSAC 2 cut(s) 80, 428
TspDTI ATGAA 3 cut(s) 156, 192, 395
TspGWI ACGGA 2 cut(s) 186, 401
VpaK11BI GGWCC 1 cut(s) 391
XapI RAATTY 1 cut(s) 244
XceI RCATGY 2 cut(s) 439, 455
XspI CTAG 2 cut(s) 135, 354
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.