pycom15g06060

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000825
Physical Location & Seq
Reverse (-)
3705189 .. 3707377
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g06060.5

Sequence Viewer

Length: 801 bp
ATGTCACGGAGGCCGGTGACTCTGAATTTGGACACGTCTCGACGGTTTGGGGACAACGGCGGAGCTCTGTTCGCAGGTTCTTCCTATTCCAAATCCAAGTCTTCTCCGATCTTATTGGTCGCCCTCGTTGCCGTGGGAGCATTGCTTCTTCTTGCGTATTCATACAGCGGCAGAGGTGGAGGTGGCAATCTTAAAGGTGTTGTTAGCAGGGTTGAAGGTGATTACTCATGCACGGTGGAGGTCCAACGAGCAATTCCCGTTTTGAAGAAAGCGTATGGCGACAGCATGCATAAGGTTTTGCACGTTGGGCCCGATACTTGTACTGTGGTTTCCAAATTGCTGAGTGAGGAAGAAACTGAAGCCTGGGGTGTGGAACCCTACGATGTAGAGGATGCTGGTAGAAGTTGTAAAGCTCTTGTGCGCAAAGACATTGTGCGTGTTGTTGATATCAAGTATCCACTTCCGTACAGGCCAAAGTCATTTTCTCTTGCACTTGTTTCGGATGCGTTGGATTATTTGTCTGAAAGATACCTCAACAGAACCCTCCCTGATTTGGCAAGGGTGTCGTCTGATGGTCTTGTGGTCTTTACAGGCTATCCTCGCAAGCAGAGAGCTAAAGCTGCTGAGGTCTCCAAATTTGGGAGGGCGGCCAAATTGAGGAGCACATCCTGGTGGTCTAAGTTTTTTGGTCAGATTAGCTTAGAAGAGAATGATGATGCCGCCAAGAAGTTCAATGAGGCAGCAACAAAGATGTCGTATGTCTCAAACTGCCAAGTTTTCCACCTCAAGTCATTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

267

Amino Acids

29.21

Weight (kDa)

9.38

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 422
Acc36I ACCTGC 1 cut(s) 65
AciI CCGC 4 cut(s) 60, 168, 647, 720
AcoI YGGCCR 1 cut(s) 648
AcsI RAATTY 2 cut(s) 25, 635
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 2 cut(s) 322, 467
AfiI CCNNNNNNNGG 3 cut(s) 553, 639, 657
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 4 cut(s) 215, 265, 733, 796
AjiI CACGTC 1 cut(s) 36
AjnI CCWGG 2 cut(s) 362, 668
AjuI GAANNNNNNNTTGG 4 cut(s) 237, 269, 466, 498
AluBI AGCT 5 cut(s) 65, 413, 614, 620, 699
AluI AGCT 5 cut(s) 65, 413, 614, 620, 699
Alw21I GWGCWC 2 cut(s) 67, 665
Alw26I GTCTC 3 cut(s) 42, 634, 766
AoxI GGCC 4 cut(s) 11, 308, 470, 648
ApaI GGGCCC 1 cut(s) 312
ApeKI GCWGC 2 cut(s) 620, 740
ApoI RAATTY 2 cut(s) 25, 635
ArsI GACNNNNNNTTYG 2 cut(s) 10, 42
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 3 cut(s) 241, 308, 309
AsuHPI GGTGA 2 cut(s) 28, 230
AvaII GGWCC 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 312
BanII GRGCYC 2 cut(s) 67, 312
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 67, 665
BbvCI CCTCAGC 1 cut(s) 624
BbvI GCAGC 2 cut(s) 607, 752
BccI CCATC 1 cut(s) 566
BceAI ACGGC 2 cut(s) 73, 116
BcgI CGANNNNNNTGC 4 cut(s) 480, 514, 546, 580
BciT130I CCWGG 2 cut(s) 364, 670
BciVI GTATCC 1 cut(s) 465
BcoDI GTCTC 3 cut(s) 42, 634, 766
BfuAI ACCTGC 1 cut(s) 65
BfuI GTATCC 1 cut(s) 465
BisI GCNGC 5 cut(s) 169, 621, 648, 720, 741
BlsI GCNGC 5 cut(s) 170, 622, 649, 721, 742
Bme1390I CCNGG 2 cut(s) 364, 670
Bme18I GGWCC 1 cut(s) 241
BmgBI CACGTC 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 241, 308, 309
BmiI GGNNCC 2 cut(s) 310, 375
BmrFI CCNGG 2 cut(s) 364, 670
BmsI GCATC 3 cut(s) 382, 493, 706
BpiI GAAGAC 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 770
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 2 cut(s) 132, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 553, 639, 657
Bse118I RCCGGY 1 cut(s) 13
Bse3DI GCAATG 1 cut(s) 140
BseBI CCWGG 2 cut(s) 364, 670
BseDI CCNNGG 2 cut(s) 132, 363
BseGI GGATG 3 cut(s) 397, 508, 665
BseLI CCNNNNNNNGG 3 cut(s) 553, 639, 657
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 2 cut(s) 332, 615
BseRI GAGGAG 1 cut(s) 673
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 2 cut(s) 607, 752
BshFI GGCC 4 cut(s) 13, 310, 472, 650
BsiHKAI GWGCWC 2 cut(s) 67, 665
BsiSI CCGG 1 cut(s) 14
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 3 cut(s) 553, 639, 657
BsmAI GTCTC 3 cut(s) 42, 634, 766
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 65
BsnI GGCC 4 cut(s) 13, 310, 472, 650
Bso31I GGTCTC 1 cut(s) 634
Bsp120I GGGCCC 1 cut(s) 308
Bsp1286I GDGCHC 3 cut(s) 67, 312, 665
Bsp143I GATC 1 cut(s) 108
BspACI CCGC 4 cut(s) 60, 168, 647, 720
BspANI GGCC 4 cut(s) 13, 310, 472, 650
BspCNI CTCAG 2 cut(s) 333, 616
BspLI GGNNCC 2 cut(s) 310, 375
BspMI ACCTGC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 132, 363
BssMI GATC 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 364, 670
Bst4CI ACNGT 3 cut(s) 45, 235, 325
Bst6I CTCTTC 1 cut(s) 699
BstC8I GCNNGC 2 cut(s) 287, 605
BstDEI CTNAG 4 cut(s) 341, 624, 678, 700
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 3 cut(s) 397, 508, 665
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 1 cut(s) 111
BstMAI GTCTC 3 cut(s) 42, 634, 766
BstMBI GATC 1 cut(s) 108
BstMWI GCNNNNNNNGC 6 cut(s) 71, 128, 137, 307, 600, 620
BstNI CCWGG 2 cut(s) 364, 670
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 2 cut(s) 362, 668
BstSLI GKGCMC 1 cut(s) 312
BstV1I GCAGC 2 cut(s) 607, 752
BstV2I GAAGAC 1 cut(s) 93
BsuI GTATCC 1 cut(s) 465
BsuRI GGCC 4 cut(s) 13, 310, 472, 650
BtgI CCRYGG 1 cut(s) 132
BtrI CACGTC 1 cut(s) 36
BtsCI GGATG 3 cut(s) 397, 508, 665
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 2 cut(s) 287, 605
CfoI GCGC 1 cut(s) 423
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 3 cut(s) 241, 308, 309
Csp6I GTAC 2 cut(s) 321, 466
CviAII CATG 2 cut(s) 228, 286
CviQI GTAC 2 cut(s) 321, 466
DdeI CTNAG 4 cut(s) 341, 624, 678, 700
DpnI GATC 1 cut(s) 110
DpnII GATC 1 cut(s) 108
EaeI YGGCCR 1 cut(s) 648
Eam1104I CTCTTC 1 cut(s) 699
EarI CTCTTC 1 cut(s) 699
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 65
Eco24I GRGCYC 2 cut(s) 67, 312
Eco31I GGTCTC 1 cut(s) 634
Eco32I GATATC 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 362, 668
EcoRV GATATC 1 cut(s) 448
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 2 cut(s) 67, 312
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 2 cut(s) 231, 289
FaiI YATR 6 cut(s) 163, 229, 276, 287, 291, 759
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 227, 285
Fnu4HI GCNGC 5 cut(s) 169, 621, 648, 720, 741
FokI GGATG 3 cut(s) 404, 515, 652
FriOI GRGCYC 2 cut(s) 67, 312
Fsp4HI GCNGC 5 cut(s) 169, 621, 648, 720, 741
FspI TGCGCA 1 cut(s) 422
GlaI GCGC 1 cut(s) 422
GluI GCNGC 5 cut(s) 169, 621, 648, 720, 741
HaeIII GGCC 4 cut(s) 13, 310, 472, 650
HapII CCGG 1 cut(s) 14
HhaI GCGC 1 cut(s) 423
Hin1II CATG 2 cut(s) 231, 289
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HinfI GANTC 1 cut(s) 19
HpaII CCGG 1 cut(s) 14
HphI GGTGA 2 cut(s) 28, 230
Hpy188I TCNGA 6 cut(s) 24, 108, 502, 523, 571, 693
Hpy188III TCNNGA 1 cut(s) 39
Hpy99I CGWCG 1 cut(s) 45
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 3 cut(s) 45, 235, 325
HpyCH4IV ACGT 2 cut(s) 35, 303
HpyCH4V TGCA 4 cut(s) 231, 289, 301, 491
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 128, 137, 307, 600, 620
HpyF3I CTNAG 4 cut(s) 341, 624, 678, 700
HpySE526I ACGT 2 cut(s) 35, 303
Hsp92II CATG 2 cut(s) 231, 289
HspAI GCGC 1 cut(s) 421
Kzo9I GATC 1 cut(s) 108
LmnI GCTCC 3 cut(s) 62, 137, 660
Lsp1109I GCAGC 2 cut(s) 607, 752
LweI GCATC 3 cut(s) 382, 493, 706
MaeII ACGT 2 cut(s) 35, 303
MaeIII GTNAC 2 cut(s) 3, 16
MalI GATC 1 cut(s) 110
MboI GATC 1 cut(s) 108
MboII GAAGA 6 cut(s) 72, 93, 140, 277, 362, 716
MfeI CAATTG 1 cut(s) 796
MhlI GDGCHC 3 cut(s) 67, 312, 665
MluCI AATT 6 cut(s) 25, 252, 335, 635, 653, 796
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 2 cut(s) 268, 489
Mph1103I ATGCAT 1 cut(s) 291
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 670
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 2 cut(s) 364, 670
MunI CAATTG 1 cut(s) 796
MvaI CCWGG 2 cut(s) 364, 670
MwoI GCNNNNNNNGC 6 cut(s) 71, 128, 137, 307, 600, 620
NdeII GATC 1 cut(s) 108
NlaIII CATG 2 cut(s) 231, 289
NlaIV GGNNCC 2 cut(s) 310, 375
NmuCI GTSAC 2 cut(s) 3, 16
NsbI TGCGCA 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 1 cut(s) 289
PaeI GCATGC 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PkrI GCNGC 5 cut(s) 170, 622, 649, 721, 742
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 362, 668
PspGI CCWGG 2 cut(s) 362, 668
PspN4I GGNNCC 2 cut(s) 310, 375
PspOMI GGGCCC 1 cut(s) 308
PspPI GGNCC 3 cut(s) 241, 308, 309
RsaI GTAC 2 cut(s) 322, 467
RsaNI GTAC 2 cut(s) 321, 466
RseI CAYNNNNRTG 1 cut(s) 670
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 5 cut(s) 169, 621, 648, 720, 741
Sau3AI GATC 1 cut(s) 108
Sau96I GGNCC 3 cut(s) 241, 308, 309
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 364, 670
SduI GDGCHC 3 cut(s) 67, 312, 665
SfaNI GCATC 3 cut(s) 382, 493, 706
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 670
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
SphI GCATGC 1 cut(s) 289
Sse9I AATT 6 cut(s) 25, 252, 335, 635, 653, 796
SsiI CCGC 4 cut(s) 60, 168, 647, 720
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 2 cut(s) 362, 668
TaaI ACNGT 3 cut(s) 45, 235, 325
TaiI ACGT 2 cut(s) 38, 306
TaqI TCGA 1 cut(s) 40
TasI AATT 6 cut(s) 25, 252, 335, 635, 653, 796
TatI WGTACW 1 cut(s) 320
TauI GCSGC 3 cut(s) 171, 650, 722
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseFI GTSAC 2 cut(s) 3, 16
TseI GCWGC 2 cut(s) 620, 740
Tsp45I GTSAC 2 cut(s) 3, 16
TspDTI ATGAA 1 cut(s) 150
TspGWI ACGGA 2 cut(s) 22, 453
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 2 cut(s) 25, 635
XceI RCATGY 1 cut(s) 289
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.