RLG00000019872

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000825
Physical Location & Seq
Reverse (-)
57807263 .. 57809100
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019872

Sequence Viewer

Length: 681 bp
ATGAGCCTTGCCTTCACTGGATCTTCGTTTGCCTCTGCGAGTGAGTCAACCTCTGTCTTCGCTGCTTCATCTTCTACGGCCGAACTGGAGCCCCGATCTCCGACGTCGAGGAGTGCTATACCAAAATCGGACCGAATTGGATTGCTTCGATTTCAGAAATGGCTCTCGCTGTTACGTCTTCTTCGTCTTGTACTGCAATCTCTGCTCGAACCTTCTCATCTGACCTCAAAGGTTTTCCTGGAAGAACTGTGCCTTTTGAGTCACTTCAAGCACTTGGATGCCATGTTGATGCAGTTTGCCCCAAAGAGAAAAGCTAGCGATGAACATATAGATATAGATAGAGGATCTCCACCTAATGAATCTTGTGCTCAACTTCCGTGGCAAACAGATTTAGTAATGGAACTGCAGTTCAGCTCAGTTCTAAATTTATCGATTGAGGAGGCAAGTGAATGCGAACCGTTCTCGGCGATTGGGGAGCATTGCTTATTCTTGCCTATTCACACAGTTGCAAAGGTGGACTTGGTAATATTAGAAGGGCTACTAGCAGGGTTCAAGGCAAGTGATTACTCATGCACAACGGAGGTCCAGCAAGCAATTCATGTTTTGAAGAAAGCATATGGTGACAGCATGCATAAGGTTTTACATGTTGGCCCTAATACTTATACTGTGCATTTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

227

Amino Acids

25.07

Weight (kDa)

5.91

Isoelectric Point (pI)

56.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 107
AclWI GGATC 2 cut(s) 28, 352
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 1 cut(s) 424
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 1 cut(s) 192
AflIII ACRYGT 1 cut(s) 643
AgsI TTSAA 3 cut(s) 268, 553, 607
AjnI CCWGG 1 cut(s) 237
AloI GAACNNNNNNTCC 2 cut(s) 392, 424
AluBI AGCT 2 cut(s) 314, 414
AluI AGCT 2 cut(s) 314, 414
Alw21I GWGCWC 1 cut(s) 370
AlwI GGATC 2 cut(s) 28, 352
AoxI GGCC 2 cut(s) 78, 649
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 1 cut(s) 424
AspS9I GGNCC 3 cut(s) 130, 583, 650
AsuHPI GGTGA 1 cut(s) 632
AsuNHI GCTAGC 1 cut(s) 314
AvaII GGWCC 2 cut(s) 130, 583
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 49, 170
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 1 cut(s) 49
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 239
BfaI CTAG 2 cut(s) 315, 542
BfmI CTRYAG 1 cut(s) 404
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 2 cut(s) 130, 583
BmgT120I GGNCC 3 cut(s) 130, 583, 650
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 2 cut(s) 268, 279
BmtI GCTAGC 1 cut(s) 318
BpiI GAAGAC 2 cut(s) 49, 170
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 1 cut(s) 107
Bsa29I ATCGAT 1 cut(s) 431
BsaHI GRCGYC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 377
Bse1I ACTGG 2 cut(s) 22, 90
Bse3DI GCAATG 1 cut(s) 478
BseBI CCWGG 1 cut(s) 239
BseCI ATCGAT 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 377
BseGI GGATG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 2 cut(s) 22, 90
BseRI GAGGAG 2 cut(s) 124, 452
BseX3I CGGCCG 1 cut(s) 78
BseXI GCAGC 1 cut(s) 49
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 2 cut(s) 80, 651
BshVI ATCGAT 1 cut(s) 431
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 2 cut(s) 80, 651
Bsp1286I GDGCHC 2 cut(s) 93, 370
Bsp143I GATC 3 cut(s) 20, 95, 344
BspANI GGCC 2 cut(s) 80, 651
BspCNI CTCAG 1 cut(s) 428
BspDI ATCGAT 1 cut(s) 431
BspLI GGNNCC 1 cut(s) 90
BspMAI CTGCAG 1 cut(s) 408
BspOI GCTAGC 1 cut(s) 318
BspPI GGATC 2 cut(s) 28, 352
BsrDI GCAATG 1 cut(s) 478
BsrI ACTGG 2 cut(s) 22, 90
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 3 cut(s) 20, 95, 344
BssNI GRCGYC 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 4 cut(s) 249, 459, 505, 667
BstACI GRCGYC 1 cut(s) 104
BstAPI GCANNNNNTGC 1 cut(s) 202
BstC8I GCNNGC 3 cut(s) 316, 591, 629
BstDEI CTNAG 1 cut(s) 415
BstDSI CCRYGG 1 cut(s) 377
BstF5I GGATG 1 cut(s) 283
BstKTI GATC 3 cut(s) 23, 98, 347
BstMBI GATC 3 cut(s) 20, 95, 344
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 2 cut(s) 631, 647
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 2 cut(s) 49, 170
BstX2I RGATCY 2 cut(s) 20, 344
BstYI RGATCY 2 cut(s) 20, 344
BstZI CGGCCG 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 431
BsuRI GGCC 2 cut(s) 80, 651
BsuTUI ATCGAT 1 cut(s) 431
BtgI CCRYGG 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 15
Cac8I GCNNGC 3 cut(s) 316, 591, 629
Cfr13I GGNCC 3 cut(s) 130, 583, 650
ClaI ATCGAT 1 cut(s) 431
CpoI CGGWCCG 1 cut(s) 130
Csp6I GTAC 1 cut(s) 191
CspCI CAANNNNNGTGG 2 cut(s) 359, 394
CspI CGGWCCG 1 cut(s) 130
CviAII CATG 5 cut(s) 283, 570, 599, 628, 644
CviJI RGCY 8 cut(s) 6, 80, 91, 163, 314, 414, 538, 651
CviKI_1 RGCY 8 cut(s) 6, 80, 91, 163, 314, 414, 538, 651
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 1 cut(s) 415
DpnI GATC 3 cut(s) 22, 97, 346
DpnII GATC 3 cut(s) 20, 95, 344
EaeI YGGCCR 1 cut(s) 78
EagI CGGCCG 1 cut(s) 78
EclXI CGGCCG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 130, 583
Eco52I CGGCCG 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 237
EcoT22I ATGCAT 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 5 cut(s) 286, 573, 602, 631, 647
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FatI CATG 5 cut(s) 282, 569, 598, 627, 643
FauNDI CATATG 1 cut(s) 616
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 2 cut(s) 315, 542
GluI GCNGC 1 cut(s) 63
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 2 cut(s) 80, 651
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 5 cut(s) 286, 573, 602, 631, 647
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 3 cut(s) 44, 259, 359
HphI GGTGA 1 cut(s) 632
Hpy166II GTNNAC 2 cut(s) 48, 517
Hpy188I TCNGA 4 cut(s) 102, 130, 156, 222
Hpy8I GTNNAC 2 cut(s) 48, 517
Hpy99I CGWCG 2 cut(s) 106, 109
HpyAV CCTTC 3 cut(s) 22, 222, 527
HpyCH4III ACNGT 4 cut(s) 249, 459, 505, 667
HpyCH4IV ACGT 2 cut(s) 104, 175
HpyCH4V TGCA 7 cut(s) 196, 292, 406, 509, 573, 631, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 1 cut(s) 415
HpySE526I ACGT 2 cut(s) 104, 175
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 5 cut(s) 286, 573, 602, 631, 647
Kzo9I GATC 3 cut(s) 20, 95, 344
LmnI GCTCC 2 cut(s) 88, 475
LpnPI CCDG 6 cut(s) 3, 71, 224, 251, 531, 599
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 2 cut(s) 268, 279
MaeI CTAG 2 cut(s) 315, 542
MaeII ACGT 2 cut(s) 104, 175
MaeIII GTNAC 3 cut(s) 171, 260, 620
MalI GATC 3 cut(s) 22, 97, 346
MboI GATC 3 cut(s) 20, 95, 344
MboII GAAGA 7 cut(s) 15, 49, 63, 170, 173, 254, 619
MflI RGATCY 2 cut(s) 20, 344
MhlI GDGCHC 2 cut(s) 93, 370
MluCI AATT 3 cut(s) 135, 424, 594
MlyI GAGTC 2 cut(s) 53, 268
MmeI TCCRAC 1 cut(s) 125
MnlI CCTC 8 cut(s) 43, 61, 102, 235, 335, 430, 433, 574
Mph1103I ATGCAT 1 cut(s) 633
MslI CAYNNNNRTG 2 cut(s) 276, 287
MspR9I CCNGG 1 cut(s) 239
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 202
NdeI CATATG 1 cut(s) 616
NdeII GATC 3 cut(s) 20, 95, 344
NheI GCTAGC 1 cut(s) 314
NlaIII CATG 5 cut(s) 286, 573, 602, 631, 647
NlaIV GGNNCC 1 cut(s) 90
NmeAIII GCCGAG 1 cut(s) 443
NmuCI GTSAC 2 cut(s) 260, 620
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 2 cut(s) 631, 647
PaeI GCATGC 1 cut(s) 631
PciI ACATGT 1 cut(s) 643
PcsI WCGNNNNNNNCGW 1 cut(s) 181
PctI GAATGC 1 cut(s) 455
PfeI GAWTC 1 cut(s) 359
PfoI TCCNGGA 1 cut(s) 237
PkrI GCNGC 1 cut(s) 64
PleI GAGTC 2 cut(s) 52, 267
PpsI GAGTC 2 cut(s) 52, 267
PscI ACATGT 1 cut(s) 643
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 3 cut(s) 130, 583, 650
PstI CTGCAG 1 cut(s) 408
PsuI RGATCY 2 cut(s) 20, 344
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 2 cut(s) 276, 287
Rsr2I CGGWCCG 1 cut(s) 130
RsrII CGGWCCG 1 cut(s) 130
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 3 cut(s) 20, 95, 344
Sau96I GGNCC 3 cut(s) 130, 583, 650
SchI GAGTC 2 cut(s) 53, 268
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 2 cut(s) 93, 370
SfaNI GCATC 2 cut(s) 268, 279
SfcI CTRYAG 1 cut(s) 404
SinI GGWCC 2 cut(s) 130, 583
SmiMI CAYNNNNRTG 2 cut(s) 276, 287
SphI GCATGC 1 cut(s) 631
Sse9I AATT 3 cut(s) 135, 424, 594
SspI AATATT 1 cut(s) 528
SspMI CTAG 2 cut(s) 315, 542
StyD4I CCNGG 1 cut(s) 237
TaaI ACNGT 4 cut(s) 249, 459, 505, 667
TaiI ACGT 2 cut(s) 107, 178
TaqI TCGA 4 cut(s) 107, 148, 207, 431
TaqII GACCGA 1 cut(s) 147
TasI AATT 3 cut(s) 135, 424, 594
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 1 cut(s) 359
TscAI CASTG 1 cut(s) 22
TseFI GTSAC 2 cut(s) 260, 620
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 2 cut(s) 260, 620
TspDTI ATGAA 4 cut(s) 57, 336, 372, 587
TspGWI ACGGA 2 cut(s) 366, 593
TspRI CASTG 1 cut(s) 22
VpaK11BI GGWCC 2 cut(s) 130, 583
XapI RAATTY 1 cut(s) 424
XceI RCATGY 2 cut(s) 631, 647
XspI CTAG 2 cut(s) 315, 542
ZraI GACGTC 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.