pycom05g10670

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000825
Physical Location & Seq
Reverse (-)
14116096 .. 14118339
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g10670.5

Sequence Viewer

Length: 786 bp
ATGTCGAGGAGGCCAGTAAATCCTTCCAGACGCATTGGTGATGGTGGAAGTATCCCATTTGTGGGTGTGGTGCAATCCAAAGCACGCTCATCACCTCTGCTGTCCATAGGACTTGTGCTTGTGGGTGCAGTTCTTCTCGTCTTCTACGCTTTTAGCGGGTCAGGTGGCCAGAGCACTAAAGAGGCTGTAATTAAACTTGAAGGTGGTTTTTCGTGTACATTTGAAGTTCAAAGAGTAATACCTATACTGAAGAAGGCATATGGTGATAGCATGCGTAAGGTGTTGCACGTGGGCCCTGAAACCTGCTCAGTGGTATCTAAATTACTAAAAGAAGAGGATACTGAAGCATGGGGTGTGGAACCATATGACTTGGAAGATGCTGACGCAAGTTGCAAGAGTCTTGTTAGCAAAGGCATTGTGCGGGCGGCTGATATAAAGTTTCCACTGCCATACCGGGCAAAATCTTTTTCTCTCGTAATAACATCGGATGCATTAGATTACTTGTCTCCAAAGTACCTCAACAGAACTCTTCCAGAGTTGGCGCGGGTATCTGCTGATGGTGTTGTTATCTTTACTGGTTATCCAGGTCAGCATAGAGCTAATGTTGCAGAGCTATCCAAATTTGGCCGTCCAGCGAAATTGCGAAGCTCATCTTGGTGGATAAGGTACTTTGTTCAAACAAGACTAGAAGAGAACGAAGTGGCCTCAAAGAAGTTTGAACAGGCTGCACTAAAGAAATCTTACACACCTACCTGCCAAGTTTTCCACCTCAAGTCATACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

262

Amino Acids

28.75

Weight (kDa)

9.48

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 311, 761
AccII CGCG 1 cut(s) 544
AciI CCGC 4 cut(s) 156, 421, 425, 544
AcoI YGGCCR 2 cut(s) 166, 625
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 2 cut(s) 269, 363
AcvI CACGTG 1 cut(s) 289
AfaI GTAC 3 cut(s) 217, 515, 668
AfiI CCNNNNNNNGG 2 cut(s) 61, 62
AgsI TTSAA 5 cut(s) 200, 224, 230, 677, 719
AjnI CCWGG 1 cut(s) 583
AjuI GAANNNNNNNTTGG 2 cut(s) 637, 669
AluBI AGCT 3 cut(s) 599, 613, 648
AluI AGCT 3 cut(s) 599, 613, 648
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 510
AoxI GGCC 5 cut(s) 11, 166, 292, 625, 702
ApaI GGGCCC 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 725
ApoI RAATTY 1 cut(s) 620
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 2 cut(s) 292, 293
AsuC2I CCSGG 1 cut(s) 455
AsuHPI GGTGA 3 cut(s) 50, 84, 275
BaeGI GKGCMC 1 cut(s) 296
BalI TGGCCA 1 cut(s) 168
BanII GRGCYC 1 cut(s) 296
BbrPI CACGTG 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 712
BccI CCATC 2 cut(s) 35, 551
BceAI ACGGC 1 cut(s) 612
BciT130I CCWGG 1 cut(s) 585
BciVI GTATCC 2 cut(s) 62, 331
BcnI CCSGG 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 1 cut(s) 686
BfuAI ACCTGC 2 cut(s) 311, 761
BfuI GTATCC 2 cut(s) 62, 331
BisI GCNGC 2 cut(s) 426, 726
BlsI GCNGC 2 cut(s) 427, 727
Bme1390I CCNGG 2 cut(s) 455, 585
BmgT120I GGNCC 2 cut(s) 292, 293
BmiI GGNNCC 2 cut(s) 294, 360
BmrFI CCNGG 2 cut(s) 455, 585
BmsI GCATC 2 cut(s) 367, 478
BpiI GAAGAC 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 1 cut(s) 455
BsaAI YACGTR 1 cut(s) 289
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 62
Bse1I ACTGG 2 cut(s) 14, 580
BseBI CCWGG 1 cut(s) 585
BseGI GGATG 1 cut(s) 493
BseLI CCNNNNNNNGG 2 cut(s) 61, 62
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 14, 580
BseRI GAGGAG 1 cut(s) 22
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 1 cut(s) 712
BsgI GTGCAG 2 cut(s) 147, 711
Bsh1236I CGCG 1 cut(s) 544
BshFI GGCC 5 cut(s) 13, 168, 294, 627, 704
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 1 cut(s) 454
BslI CCNNNNNNNGG 2 cut(s) 61, 62
BsmAI GTCTC 1 cut(s) 510
BsnI GGCC 5 cut(s) 13, 168, 294, 627, 704
Bsp120I GGGCCC 1 cut(s) 292
Bsp1286I GDGCHC 2 cut(s) 176, 296
Bsp1407I TGTACA 1 cut(s) 215
BspACI CCGC 4 cut(s) 156, 421, 425, 544
BspANI GGCC 5 cut(s) 13, 168, 294, 627, 704
BspCNI CTCAG 1 cut(s) 320
BspFNI CGCG 1 cut(s) 544
BspLI GGNNCC 2 cut(s) 294, 360
BspMI ACCTGC 2 cut(s) 311, 761
BsrGI TGTACA 1 cut(s) 215
BsrI ACTGG 2 cut(s) 14, 580
Bst2UI CCWGG 1 cut(s) 585
Bst6I CTCTTC 3 cut(s) 327, 534, 684
BstAUI TGTACA 1 cut(s) 215
BstBAI YACGTR 1 cut(s) 289
BstC8I GCNNGC 3 cut(s) 85, 272, 423
BstDEI CTNAG 1 cut(s) 307
BstF5I GGATG 1 cut(s) 493
BstFNI CGCG 1 cut(s) 544
BstHHI GCGC 1 cut(s) 544
BstMAI GTCTC 1 cut(s) 510
BstMWI GCNNNNNNNGC 1 cut(s) 605
BstNI CCWGG 1 cut(s) 585
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 2 cut(s) 453, 583
BstSLI GKGCMC 1 cut(s) 296
BstUI CGCG 1 cut(s) 544
BstV1I GCAGC 1 cut(s) 712
BstV2I GAAGAC 1 cut(s) 133
BsuI GTATCC 2 cut(s) 62, 331
BsuRI GGCC 5 cut(s) 13, 168, 294, 627, 704
BtsCI GGATG 1 cut(s) 493
BtsI GCAGTG 1 cut(s) 443
BtsIMutI CAGTG 2 cut(s) 315, 443
BveI ACCTGC 2 cut(s) 311, 761
Cac8I GCNNGC 3 cut(s) 85, 272, 423
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 2 cut(s) 292, 293
CseI GACGC 2 cut(s) 39, 392
Csp6I GTAC 3 cut(s) 216, 514, 667
CviAII CATG 2 cut(s) 271, 348
CviQI GTAC 3 cut(s) 216, 514, 667
DdeI CTNAG 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 166, 625
Eam1104I CTCTTC 3 cut(s) 327, 534, 684
EarI CTCTTC 3 cut(s) 327, 534, 684
Eco24I GRGCYC 1 cut(s) 296
Eco57I CTGAAG 2 cut(s) 269, 363
Eco72I CACGTG 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 583
EcoT22I ATGCAT 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 296
FaeI CATG 2 cut(s) 274, 351
FalI AAGNNNNNCTT 2 cut(s) 637, 669
FatI CATG 2 cut(s) 270, 347
FauI CCCGC 3 cut(s) 149, 414, 537
FauNDI CATATG 2 cut(s) 259, 364
Fnu4HI GCNGC 2 cut(s) 426, 726
FokI GGATG 1 cut(s) 500
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 426, 726
FspBI CTAG 1 cut(s) 686
GlaI GCGC 1 cut(s) 543
GluI GCNGC 2 cut(s) 426, 726
HaeIII GGCC 5 cut(s) 13, 168, 294, 627, 704
HapII CCGG 1 cut(s) 454
HgaI GACGC 2 cut(s) 39, 392
HhaI GCGC 1 cut(s) 544
Hin1II CATG 2 cut(s) 274, 351
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HinfI GANTC 1 cut(s) 397
HpaII CCGG 1 cut(s) 454
HphI GGTGA 3 cut(s) 50, 84, 275
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 1 cut(s) 487
Hpy188III TCNNGA 2 cut(s) 27, 533
Hpy8I GTNNAC 1 cut(s) 216
HpyAV CCTTC 3 cut(s) 33, 194, 247
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 7 cut(s) 73, 128, 286, 393, 491, 608, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 605
HpyF3I CTNAG 1 cut(s) 307
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 2 cut(s) 274, 351
HspAI GCGC 1 cut(s) 542
Lsp1109I GCAGC 1 cut(s) 712
LweI GCATC 2 cut(s) 367, 478
MaeI CTAG 1 cut(s) 686
MaeII ACGT 1 cut(s) 288
MboII GAAGA 7 cut(s) 125, 133, 262, 344, 386, 521, 701
MhlI GDGCHC 2 cut(s) 176, 296
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 4 cut(s) 189, 320, 620, 638
MluNI TGGCCA 1 cut(s) 168
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 7 cut(s) 3, 105, 175, 328, 527, 715, 779
Mox20I TGGCCA 1 cut(s) 168
Mph1103I ATGCAT 1 cut(s) 493
MscI TGGCCA 1 cut(s) 168
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 655
Msp20I TGGCCA 1 cut(s) 168
MspI CCGG 1 cut(s) 454
MspR9I CCNGG 2 cut(s) 455, 585
MvaI CCWGG 1 cut(s) 585
MvnI CGCG 1 cut(s) 544
MwoI GCNNNNNNNGC 1 cut(s) 605
NciI CCSGG 1 cut(s) 455
NdeI CATATG 2 cut(s) 259, 364
NlaIII CATG 2 cut(s) 274, 351
NlaIV GGNNCC 2 cut(s) 294, 360
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 1 cut(s) 274
PaeI GCATGC 1 cut(s) 274
PkrI GCNGC 2 cut(s) 427, 727
PleI GAGTC 1 cut(s) 405
PmaCI CACGTG 1 cut(s) 289
PmlI CACGTG 1 cut(s) 289
PpsI GAGTC 1 cut(s) 405
Ppu21I YACGTR 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 583
PspCI CACGTG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 583
PspN4I GGNNCC 2 cut(s) 294, 360
PspOMI GGGCCC 1 cut(s) 292
PspPI GGNCC 2 cut(s) 292, 293
RsaI GTAC 3 cut(s) 217, 515, 668
RsaNI GTAC 3 cut(s) 216, 514, 667
RseI CAYNNNNRTG 1 cut(s) 655
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 426, 726
Sau96I GGNCC 2 cut(s) 292, 293
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 2 cut(s) 455, 585
SduI GDGCHC 2 cut(s) 176, 296
SfaNI GCATC 2 cut(s) 367, 478
SmiMI CAYNNNNRTG 1 cut(s) 655
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SphI GCATGC 1 cut(s) 274
Sse9I AATT 4 cut(s) 189, 320, 620, 638
SsiI CCGC 4 cut(s) 156, 421, 425, 544
SspMI CTAG 1 cut(s) 686
StyD4I CCNGG 2 cut(s) 453, 583
TaiI ACGT 1 cut(s) 291
TaqI TCGA 1 cut(s) 5
TasI AATT 4 cut(s) 189, 320, 620, 638
TatI WGTACW 1 cut(s) 215
TauI GCSGC 1 cut(s) 428
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 2 cut(s) 315, 450
TseI GCWGC 1 cut(s) 725
TspRI CASTG 2 cut(s) 315, 450
XapI RAATTY 1 cut(s) 620
XceI RCATGY 1 cut(s) 274
XspI CTAG 1 cut(s) 686
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.