Rw6G042820

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000825
Physical Location & Seq
Forward (+)
66210673 .. 66217123
6451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G042820.1

Sequence Viewer

Length: 612 bp
ATGGGAGCATTGCTTATTCTTGCCTATTCACACAGTGGCAAAGGTGGACTTGGTAATACTAGAGGGGCTATTAGCAGGGTTCAAGGTGATTACTCATGCACAGCGGAGGTCCAGCGAGCAATTCCTTTTTTGAAGAAAGCATATGGTGACAGCATGCATAAGGTTTTGCACGTTGGCCCTGATACTTGTACAGTGGTTTCTAAATTGATGCTGATAGAAGTTGCAAAGCTCTTGCCTGATAGAAGTTGCAAAGCTCTTGTGCGCAAAGCCACTTCCATACAGGCCAAAATTATTTTTGTTGTCATTGTTTTGGATTCTTTGGATTATTTGTCTGAAAGATACCTCAACAAGACCCTTCTGGATCTGGTTAGGGTATCAGCTGATGGTGTTGTAATCTTTACAGGCTCTCCTGGAAAGCAGAGAGCTAAAGCAGCTGAGGTCTCCAAATTTGGTAGGGCGGCTAAATTGAGGAACACAGCCTGGTGGACTAAGTTTTTTTGGAAGGTTAGCTTAGAAGAGAACGAAGGTGCTAGCAAGAAGTTTAATATTGCTGCAACAAAGATGTCATTTGTCTCAAATTGTCAAGTTTTCCACCTCAGGTCATTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

203

Amino Acids

22.35

Weight (kDa)

9.88

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 263
AciI CCGC 2 cut(s) 104, 458
AclWI GGATC 1 cut(s) 369
AcsI RAATTY 1 cut(s) 446
AdeI CACNNNGTG 1 cut(s) 35
AfaI GTAC 1 cut(s) 190
AgsI TTSAA 3 cut(s) 83, 133, 607
AjnI CCWGG 2 cut(s) 409, 479
AluBI AGCT 6 cut(s) 229, 254, 380, 425, 434, 510
AluI AGCT 6 cut(s) 229, 254, 380, 425, 434, 510
Alw26I GTCTC 2 cut(s) 445, 577
AlwI GGATC 1 cut(s) 369
AoxI GGCC 2 cut(s) 175, 282
ApeKI GCWGC 2 cut(s) 431, 551
ApoI RAATTY 1 cut(s) 446
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 2 cut(s) 109, 176
AsuHPI GGTGA 2 cut(s) 98, 158
AsuNHI GCTAGC 1 cut(s) 530
AvaII GGWCC 1 cut(s) 109
AxyI CCTNAGG 1 cut(s) 596
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 2 cut(s) 443, 538
BccI CCATC 1 cut(s) 377
BciT130I CCWGG 2 cut(s) 411, 481
BcoDI GTCTC 2 cut(s) 445, 577
BfaI CTAG 2 cut(s) 60, 531
BisI GCNGC 3 cut(s) 432, 459, 552
BlsI GCNGC 3 cut(s) 433, 460, 553
Bme1390I CCNGG 2 cut(s) 411, 481
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 109, 176
BmrFI CCNGG 2 cut(s) 411, 481
BmsI GCATC 1 cut(s) 198
BmtI GCTAGC 1 cut(s) 534
Bpu10I CCTNAGC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 445
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bse21I CCTNAGG 1 cut(s) 596
Bse3DI GCAATG 1 cut(s) 8
BseBI CCWGG 2 cut(s) 411, 481
BseMI GCAATG 1 cut(s) 8
BseMII CTCAG 2 cut(s) 426, 610
BseXI GCAGC 2 cut(s) 443, 538
BshFI GGCC 2 cut(s) 177, 284
BsmAI GTCTC 2 cut(s) 445, 577
BsnI GGCC 2 cut(s) 177, 284
Bso31I GGTCTC 1 cut(s) 445
Bsp1407I TGTACA 1 cut(s) 188
Bsp143I GATC 1 cut(s) 361
BspACI CCGC 2 cut(s) 104, 458
BspANI GGCC 2 cut(s) 177, 284
BspCNI CTCAG 2 cut(s) 427, 609
BspOI GCTAGC 1 cut(s) 534
BspPI GGATC 1 cut(s) 369
BspTNI GGTCTC 1 cut(s) 445
BsrDI GCAATG 1 cut(s) 8
BsrGI TGTACA 1 cut(s) 188
BssMI GATC 1 cut(s) 361
Bst2UI CCWGG 2 cut(s) 411, 481
Bst4CI ACNGT 2 cut(s) 35, 193
Bst6I CTCTTC 1 cut(s) 510
BstAUI TGTACA 1 cut(s) 188
BstC8I GCNNGC 3 cut(s) 117, 155, 532
BstDEI CTNAG 4 cut(s) 435, 489, 511, 596
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 1 cut(s) 364
BstMAI GTCTC 2 cut(s) 445, 577
BstMBI GATC 1 cut(s) 361
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNI CCWGG 2 cut(s) 411, 481
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 2 cut(s) 409, 479
BstV1I GCAGC 2 cut(s) 443, 538
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 2 cut(s) 177, 284
BtsIMutI CAGTG 2 cut(s) 40, 198
Cac8I GCNNGC 3 cut(s) 117, 155, 532
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 2 cut(s) 109, 176
Csp6I GTAC 1 cut(s) 189
CviAII CATG 2 cut(s) 96, 154
CviQI GTAC 1 cut(s) 189
DdeI CTNAG 4 cut(s) 435, 489, 511, 596
DpnI GATC 1 cut(s) 363
DpnII GATC 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 109
Eco81I CCTNAGG 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 409, 479
EcoT22I ATGCAT 1 cut(s) 159
FaeI CATG 2 cut(s) 99, 157
FaiI YATR 6 cut(s) 97, 142, 144, 155, 159, 278
FalI AAGNNNNNCTT 4 cut(s) 33, 65, 494, 526
FatI CATG 2 cut(s) 95, 153
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 3 cut(s) 432, 459, 552
Fsp4HI GCNGC 3 cut(s) 432, 459, 552
FspBI CTAG 2 cut(s) 60, 531
FspI TGCGCA 1 cut(s) 263
GlaI GCGC 1 cut(s) 263
GluI GCNGC 3 cut(s) 432, 459, 552
HaeIII GGCC 2 cut(s) 177, 284
HhaI GCGC 1 cut(s) 264
Hin1II CATG 2 cut(s) 99, 157
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HinfI GANTC 1 cut(s) 314
HphI GGTGA 2 cut(s) 98, 158
Hpy166II GTNNAC 2 cut(s) 47, 486
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 2 cut(s) 47, 486
HpyAV CCTTC 3 cut(s) 365, 496, 518
HpyCH4III ACNGT 2 cut(s) 35, 193
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 6 cut(s) 99, 157, 169, 224, 249, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 4 cut(s) 435, 489, 511, 596
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 2 cut(s) 99, 157
HspAI GCGC 1 cut(s) 262
Kzo9I GATC 1 cut(s) 361
LmnI GCTCC 1 cut(s) 5
Lsp1109I GCAGC 2 cut(s) 443, 538
LweI GCATC 1 cut(s) 198
MaeI CTAG 2 cut(s) 60, 531
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 146
MalI GATC 1 cut(s) 363
MboI GATC 1 cut(s) 361
MboII GAAGA 2 cut(s) 145, 527
MfeI CAATTG 1 cut(s) 607
MflI RGATCY 1 cut(s) 361
MluCI AATT 7 cut(s) 120, 203, 288, 446, 464, 577, 607
MnlI CCTC 6 cut(s) 56, 100, 353, 430, 462, 605
Mph1103I ATGCAT 1 cut(s) 159
MseI TTAA 1 cut(s) 543
MspA1I CMGCKG 3 cut(s) 104, 380, 434
MspR9I CCNGG 2 cut(s) 411, 481
MunI CAATTG 1 cut(s) 607
MvaI CCWGG 2 cut(s) 411, 481
MwoI GCNNNNNNNGC 1 cut(s) 431
NdeI CATATG 1 cut(s) 142
NdeII GATC 1 cut(s) 361
NheI GCTAGC 1 cut(s) 530
NlaIII CATG 2 cut(s) 99, 157
NmuCI GTSAC 1 cut(s) 146
NsbI TGCGCA 1 cut(s) 263
NsiI ATGCAT 1 cut(s) 159
NspI RCATGY 1 cut(s) 157
PaeI GCATGC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 409
PkrI GCNGC 3 cut(s) 433, 460, 553
Psp6I CCWGG 2 cut(s) 409, 479
PspGI CCWGG 2 cut(s) 409, 479
PspPI GGNCC 2 cut(s) 109, 176
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 2 cut(s) 380, 434
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 3 cut(s) 432, 459, 552
Sau3AI GATC 1 cut(s) 361
Sau96I GGNCC 2 cut(s) 109, 176
ScrFI CCNGG 2 cut(s) 411, 481
SfaNI GCATC 1 cut(s) 198
SinI GGWCC 1 cut(s) 109
SphI GCATGC 1 cut(s) 157
Sse9I AATT 7 cut(s) 120, 203, 288, 446, 464, 577, 607
SsiI CCGC 2 cut(s) 104, 458
SspI AATATT 1 cut(s) 547
SspMI CTAG 2 cut(s) 60, 531
StyD4I CCNGG 2 cut(s) 409, 479
TaaI ACNGT 2 cut(s) 35, 193
TaiI ACGT 1 cut(s) 174
TasI AATT 7 cut(s) 120, 203, 288, 446, 464, 577, 607
TatI WGTACW 1 cut(s) 188
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 2 cut(s) 40, 198
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 2 cut(s) 431, 551
Tsp45I GTSAC 1 cut(s) 146
TspRI CASTG 2 cut(s) 40, 198
VpaK11BI GGWCC 1 cut(s) 109
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 157
XspI CTAG 2 cut(s) 60, 531
Zsp2I ATGCAT 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.