Prupe.8G155700_v2.0.a1

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000825
Physical Location & Seq
Reverse (-)
16689246 .. 16692119
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G155700.1

Sequence Viewer

Length: 786 bp
ATGTCAAGGAGGCCAGTAAATCCTGCTAGACGCATTGGCGACGGTGGAAGCATCCCATTTGTGGGTGTTGTGCAGTCCAAAGCACGCTCATCGCCACTACTGTCTATAGGGCTTGTGCTTGTGGGTGCAATTCTTCTTGTCTGCTATGCTTTCAGTGGGTCAGGTGGACGGAGCAGTAAAGAGGCTGTGATTAAACTTGAAGGTGGTGCTTCGTGCACATTCGAAGTTCAGAGAGCAATACCCATACTAAAGAAAGCATATGGTGATAGCATGAAGAAAGTGTTGCATGTGGGCCCTGACACCTGTTCAGTGGTATCTAAATTATTAAAAGAGGAGGATACGGAAGCCTGGGGTGTGGAACCATTTGACTTAGAAGATGCTGATGCAAATTGCAAGAGTCTCGTGAGCAAAGGCATTGTGCGTGCTGCTGATATAAAGTTTTCTCTGCCTTATCGGCCAAAATCATTTTCTCTCGTAATAGCATCAGATGCGTTAGATTACTTGTCTCCAAAGTACCTCAACAAAACTCTTCCAGAGCTGGCGAGGGTATCTGCTGATGGTGTTGTTATATTTACTGGTTATCCAGGTCAGCATAAAGCTAAAGTTGCAGAGCTATCCAAATTTGGCCGTCCAGCCAAATTGCGAAGCTCGTCCTGGTGGATAAGGTACTTTGTTCAAACTAGCTTAGAAGAGAACGAAGTAGCCTCAAAGAAGTTTGAACAGGCTGCACTAAAGAAATCTTACACGCCTGCTTGCCAAGTTTTCCACCTCAAGTCATACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

262

Amino Acids

28.43

Weight (kDa)

9.45

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 455, 625
AcsI RAATTY 1 cut(s) 620
AfaI GTAC 2 cut(s) 515, 668
AfiI CCNNNNNNNGG 2 cut(s) 61, 62
AgsI TTSAA 3 cut(s) 200, 677, 719
AjnI CCWGG 3 cut(s) 347, 583, 653
AjuI GAANNNNNNNTTGG 2 cut(s) 451, 483
AluBI AGCT 5 cut(s) 538, 599, 613, 648, 684
AluI AGCT 5 cut(s) 538, 599, 613, 648, 684
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 2 cut(s) 404, 510
Alw44I GTGCAC 1 cut(s) 214
AoxI GGCC 4 cut(s) 11, 292, 455, 625
ApaI GGGCCC 1 cut(s) 296
ApaLI GTGCAC 1 cut(s) 214
ApeKI GCWGC 2 cut(s) 425, 725
ApoI RAATTY 1 cut(s) 620
AspS9I GGNCC 2 cut(s) 292, 293
AsuHPI GGTGA 1 cut(s) 275
AsuII TTCGAA 1 cut(s) 222
BaeGI GKGCMC 2 cut(s) 218, 296
BanII GRGCYC 1 cut(s) 296
BauI CACGAG 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 2 cut(s) 412, 712
BccI CCATC 1 cut(s) 551
BceAI ACGGC 1 cut(s) 612
BcgI CGANNNNNNTGC 4 cut(s) 72, 106, 382, 416
BciT130I CCWGG 3 cut(s) 349, 585, 655
BciVI GTATCC 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 404, 510
BfaI CTAG 2 cut(s) 27, 681
BfmI CTRYAG 1 cut(s) 105
BfuI GTATCC 1 cut(s) 331
BglI GCCNNNNNGGC 1 cut(s) 454
BisI GCNGC 2 cut(s) 426, 726
BlsI GCNGC 2 cut(s) 427, 727
Bme1390I CCNGG 3 cut(s) 349, 585, 655
BmgT120I GGNCC 2 cut(s) 292, 293
BmiI GGNNCC 2 cut(s) 294, 360
BmrFI CCNGG 3 cut(s) 349, 585, 655
BmsI GCATC 5 cut(s) 60, 367, 373, 478, 491
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 62
Bse1I ACTGG 2 cut(s) 14, 580
BseBI CCWGG 3 cut(s) 349, 585, 655
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 1 cut(s) 51
BseLI CCNNNNNNNGG 2 cut(s) 61, 62
BseNI ACTGG 2 cut(s) 14, 580
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 2 cut(s) 218, 296
BseXI GCAGC 2 cut(s) 412, 712
BsgI GTGCAG 2 cut(s) 92, 711
BshFI GGCC 4 cut(s) 13, 294, 457, 627
BsiHKAI GWGCWC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 61, 62
BsmAI GTCTC 2 cut(s) 404, 510
BsnI GGCC 4 cut(s) 13, 294, 457, 627
Bsp119I TTCGAA 1 cut(s) 222
Bsp120I GGGCCC 1 cut(s) 292
Bsp1286I GDGCHC 2 cut(s) 218, 296
BspANI GGCC 4 cut(s) 13, 294, 457, 627
BspLI GGNNCC 2 cut(s) 294, 360
BspT104I TTCGAA 1 cut(s) 222
BsrI ACTGG 2 cut(s) 14, 580
BssECI CCNNGG 1 cut(s) 348
BssSI CACGAG 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 401
Bst2UI CCWGG 3 cut(s) 349, 585, 655
Bst4CI ACNGT 2 cut(s) 44, 102
Bst6I CTCTTC 2 cut(s) 534, 684
BstAPI GCANNNNNTGC 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 5 cut(s) 85, 423, 540, 750, 754
BstDEI CTNAG 2 cut(s) 370, 685
BstF5I GGATG 1 cut(s) 51
BstMAI GTCTC 2 cut(s) 404, 510
BstMWI GCNNNNNNNGC 3 cut(s) 454, 488, 605
BstNI CCWGG 3 cut(s) 349, 585, 655
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 347, 583, 653
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 2 cut(s) 218, 296
BstV1I GCAGC 2 cut(s) 412, 712
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 4 cut(s) 13, 294, 457, 627
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 1 cut(s) 51
BtsIMutI CAGTG 2 cut(s) 160, 315
Cac8I GCNNGC 5 cut(s) 85, 423, 540, 750, 754
Cfr13I GGNCC 2 cut(s) 292, 293
CseI GACGC 1 cut(s) 39
Csp6I GTAC 2 cut(s) 514, 667
CviAII CATG 2 cut(s) 271, 287
CviQI GTAC 2 cut(s) 514, 667
DdeI CTNAG 2 cut(s) 370, 685
EaeI YGGCCR 2 cut(s) 455, 625
Eam1104I CTCTTC 2 cut(s) 534, 684
EarI CTCTTC 2 cut(s) 534, 684
Eco24I GRGCYC 1 cut(s) 296
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 3 cut(s) 347, 583, 653
EcoT38I GRGCYC 1 cut(s) 296
FaeI CATG 2 cut(s) 274, 290
FatI CATG 2 cut(s) 270, 286
FauNDI CATATG 1 cut(s) 259
Fnu4HI GCNGC 2 cut(s) 426, 726
FokI GGATG 1 cut(s) 38
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 426, 726
FspBI CTAG 2 cut(s) 27, 681
GluI GCNGC 2 cut(s) 426, 726
HaeIII GGCC 4 cut(s) 13, 294, 457, 627
HgaI GACGC 1 cut(s) 39
Hin1II CATG 2 cut(s) 274, 290
HinfI GANTC 1 cut(s) 397
HphI GGTGA 1 cut(s) 275
Hpy166II GTNNAC 2 cut(s) 167, 216
Hpy188I TCNGA 2 cut(s) 231, 487
Hpy188III TCNNGA 2 cut(s) 403, 533
Hpy8I GTNNAC 2 cut(s) 167, 216
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 194
HpyCH4III ACNGT 2 cut(s) 44, 102
HpyCH4V TGCA 8 cut(s) 73, 128, 216, 286, 386, 393, 608, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 454, 488, 605
HpyF3I CTNAG 2 cut(s) 370, 685
Hsp92II CATG 2 cut(s) 274, 290
LmnI GCTCC 1 cut(s) 171
Lsp1109I GCAGC 2 cut(s) 412, 712
LweI GCATC 5 cut(s) 60, 367, 373, 478, 491
MaeI CTAG 2 cut(s) 27, 681
MboII GAAGA 5 cut(s) 125, 286, 386, 521, 701
MhlI GDGCHC 2 cut(s) 218, 296
MluCI AATT 5 cut(s) 129, 320, 388, 620, 638
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 8 cut(s) 3, 175, 325, 328, 527, 537, 715, 779
MseI TTAA 3 cut(s) 192, 326, 784
MspR9I CCNGG 3 cut(s) 349, 585, 655
MvaI CCWGG 3 cut(s) 349, 585, 655
MwoI GCNNNNNNNGC 3 cut(s) 454, 488, 605
NdeI CATATG 1 cut(s) 259
NlaIII CATG 2 cut(s) 274, 290
NlaIV GGNNCC 2 cut(s) 294, 360
NspI RCATGY 1 cut(s) 290
NspV TTCGAA 1 cut(s) 222
PkrI GCNGC 2 cut(s) 427, 727
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
Psp6I CCWGG 3 cut(s) 347, 583, 653
PspGI CCWGG 3 cut(s) 347, 583, 653
PspN4I GGNNCC 2 cut(s) 294, 360
PspOMI GGGCCC 1 cut(s) 292
PspPI GGNCC 2 cut(s) 292, 293
RsaI GTAC 2 cut(s) 515, 668
RsaNI GTAC 2 cut(s) 514, 667
SaqAI TTAA 3 cut(s) 192, 326, 784
SatI GCNGC 2 cut(s) 426, 726
Sau96I GGNCC 2 cut(s) 292, 293
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 3 cut(s) 349, 585, 655
SduI GDGCHC 2 cut(s) 218, 296
SfaNI GCATC 5 cut(s) 60, 367, 373, 478, 491
SfcI CTRYAG 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 5 cut(s) 129, 320, 388, 620, 638
SspMI CTAG 2 cut(s) 27, 681
StyD4I CCNGG 3 cut(s) 347, 583, 653
TaaI ACNGT 2 cut(s) 44, 102
TaqI TCGA 1 cut(s) 222
TasI AATT 5 cut(s) 129, 320, 388, 620, 638
Tru1I TTAA 3 cut(s) 192, 326, 784
Tru9I TTAA 3 cut(s) 192, 326, 784
TscAI CASTG 2 cut(s) 160, 315
TseI GCWGC 2 cut(s) 425, 725
TspDTI ATGAA 1 cut(s) 287
TspGWI ACGGA 2 cut(s) 184, 356
TspRI CASTG 2 cut(s) 160, 315
VneI GTGCAC 1 cut(s) 214
XapI RAATTY 1 cut(s) 620
XceI RCATGY 1 cut(s) 290
XspI CTAG 2 cut(s) 27, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.